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17 results on '"Mancino, M. G."'

1. Italian practical clinical guidelines on cholangiocarcinoma: Part II, treatment

Author

Alvaro, D., Hassan, C., Cardinale, V., Carpino, G., Fabris, L., Gringeri, E., Granata, V., Mutignani, M., Morement, H., Giuliante, F., Guglielmi, A., Ridola, L., Tonini, G., Marzioni, M., Grazi, G., Guido, M., Di Giulio, E., Pantano, F., Venere, R., Bragazzi, M. C., Biancanello, F., Faccioli, J., Giannetti, A., Cintolo, M., Di Giunta, M., Gambato, M., Lasagni, A., Izzo, F., Avallone, A., Banales, J., Rossi, M., Catalano, C., Laghi, A., D'Amati, G., and Mancino, M. G.

Subjects
surgery, distal cholangiocarcinoma, intrahepatic cholangiocarcinoma, liver transplantation, locoregional treatments, perihilar cholangiocarcinoma, target therapies
Published
2020

2. Interobserver agreement in contrast harmonic endoscopic ultrasound

Author

FUSAROLI, PIETRO, KYPRAIOS, DIMITRIOS, CALETTI, GIANCARLO, Mancino M. G., Spada A., Benini M. C., Bianchi M., Bocus P., De Angelis C., De Luca L., Fabbri C., Grillo A., Marzioni M., Reggio D., Togliani T., Zanarini S., Fusaroli P., Kypraios D., Mancino M.G., Spada A., Benini M.C., Bianchi M., Bocus P., De Angelis C., De Luca L., Fabbri C., Grillo A., Marzioni M., Reggio D., Togliani T., Zanarini S., and Caletti G.

Subjects
Adult, Aged, 80 and over, Male, Observer Variation, Sulfur Hexafluoride, Contrast Media, Middle Aged, Endosonography, ENDOSCOPIC ULTRASONOGRAPHY, Pancreatic Neoplasms, Young Adult, Humans, Female, Clinical Competence, Pancreatic Cyst, Phospholipids, Aged
Abstract

Background and Aim: Contrast harmonic endoscopic ultrasound (CH-EUS) was recently introduced to clinical practice; its reproducibility among endosonographers is unknown. Our aim was to assess the interobserver agreement (IA) in CH-EUS. Methods: Fifteen endosonographers (eight experienced and seven non-experienced) from 11 Italian EUS centers evaluated 80 video-cases (40 solid pancreatic lesions, 20 pancreatic cystic lesions and 20 submucosal lesions) of CH-EUS, according to the degree of enhancement, the pattern of distribution and the washout of the contrast agent. IA within each group and between the two groups of observers was assessed with the Fleiss kappa statistic. Results: Overall IA was moderate for the uptake and fair for the pattern of distribution and the washout. In solid pancreatic lesions, IA was moderate for the uptake and fair for the pattern and the washout. In cystic pancreatic lesions, IA was uniformly moderate for the assessment of uptake, slight for the pattern and fair for the washout. In submucosal tumors, IA was substantial for the uptake, slight for the pattern and fair for the washout. Non-experienced endosonographers demonstrated, in most cases, comparable IA with the experienced ones. Conclusions: Interobserver agreement among endosonographers for CH EUS was satisfactory. In particular, overall IA varied from slight to substantial, being fair in the majority of cases. Inherent structural features of the lesions, as well as technical differences between the variables assessed, could have accounted for the fluctuation of the results. Outcomes of IA were reproducible between experienced and non-experienced endosonographers.

Published
2012

3. Contrast harmonic enhanced EUS: technique and clinical outcome

Author

FUSAROLI, PIETRO, Mancino M. G., CALETTI, GIANCARLO, Fusaroli P., Mancino M.G., and Caletti G.

Subjects
ENDOSCOPIC ULTRASONOGRAPHY
Abstract

The use of ultrasound contrast agents is widespread in transabdominal ultrasound bringing a relevant clinical impact. Endoscopic ultrasound is very accurate but some limitations exist. For this reason, image enhancement with ultrasound contrast agents is expected to bring significant results also to endoscopic ultrasound. Contrast enhanced endoscopic ultrasound is performed at a high technical level with a dedicated harmonic echo; for this purpose radial and linear echoendoscopes can be used with a dedicated software. This new technique detects strong echosignals from microbubbles in vessels with very slow flow, without artifacts. In clinical experiences, the finding of a hypoenhancing mass with inhomogeneous pattern is a sensitive and accurate parameter to identify patients affected from adenocarcinoma. On the other hand, the finding of a hyperenhanced mass tends to rule out adenocarcinoma and is more suggestive of neuroendocrine tumor. Contrast harmonic enhanced endoscopic ultrasound can be used to select the lesions and/or areas within the lesion to puncture with fine needle aspiration in order to maximize the diagnostic yield.

Published
2011

10. Cholangiocyte proliferation

Author

Alvaro, Domenico, Alpini, G., Onori, Paolo, Franchitto, Antonio, Mancino, M. G., DRUDI METALLI, V., Barbaro, B., Gigliozzi, A., Attili, Adolfo Francesco, and Gaudio, Eugenio

Published
2003

13. Emerging from gastroesophageal reflux (Emerge): An Italian survey - II the viewpoint of the patient

Author

Bianchetti, M., Peralta, S., Nicita, R., Aragona, S. E., Ciprandi, G., Arrigoni, A., Artuso, D., Astegiano, M., Azzinnari, C., Battaglia, E., Belcari, C., Bendia, E., Benedicenti, P., Brandimarte, G., Buoncompagni, I., Cabras, M., Camilleri, S., Capece, G., Caronna, S., Cassieri, C., Castaldo, F., Catalano, T., Citarella, C., D’amore, F., D’alia, G., D’arpa, F., Dattola, A., Bortoli, N., Medici, A., Di Marzo, S., Di Mitri, R., Di Napoli, A., D’onofrio, V., Dughera, L., Elisei, W., Errico, G., Familiari, L., Familiari, P., Frasca, R., Frunzio, A., Gatti, M., Genova, S., Grosso, S., Gullotta, R., Iannuzziello, D. R., Indennitate, G., Leonardi, G., Luigiano, C., Macchiarella, B., Maisto, T., Mancino, M. G., Mancino, A., Manes, G., Marin, R., Mastinu, G. R., Moi, A., Montalbano, L. M., Monterosso, N., Morabito Loprete, A., Morlando, L., Ogliari, C., Paiano Primaldo, N., Paliani, G., Palieri, A. P., Pardocchi, D., Pati, A., Pedretti, G., Pisani, A., Plomaritis, P., Privitera, A. C., Pumpo, R., Quatraro, F., Raimondo, D., Rivellini, G., Rizzo Giovanni, L., Romano, M., Salerno, R., Edoardo Savarino, Scarpulla, G., Sinagra, E., Soncini, M., Tammaro, G., Trovato, C., Vassallo, R., Vinti, M., and Virgilio, C. M. P.

14. Correlation between the reflux finding score and the gerd impact scale in patients with gastroesophageal reflux disease

Author

Bianchetti, M., Peralta, S., Nicita, R., Salvatore Emanuele Aragona, Ciprandi, G., Arrigoni, A., Artuso, D., Astegiano, M., Azzinnari, C., Battaglia, E., Belcari, C., Bendia, E., Benedicenti, P., Brandimarte, G., Buoncompagni, I., Cabras, M., Camilleri, S., Capece, G., Caronna, S., Cassieri, C., Castaldo, F., Catalano, T., Citarella, C., D’amore, F., D’alia, G., D’arpa, F., Dattola, A., Bortoli, N., Medici, A., Di Marzo, S., Di Mitri, R., Di Napoli, A., D Onofrio, V., Dughera, L., Elisei, W., Errico, G., Familiari, L., Familiari, P., Frasca, R., Frunzio, A., Gatti, M., Genova, S., Grosso, S., Gullotta, R., Iannuzziello, D. R., Indennitate, G., Leonardi, G., Luigiano, C., Macchiarella, B., Maisto, T., Mancino, M. G., Mancino, A., Manes, G., Marin, R., Mastinu, G. R., Moi, A., Montalbano, L. M., Monterosso, N., Morabito Loprete, A., Morlando, L., Ogliari, C., Paiano Primaldo, N., Paliani, G., Palieri, A. P., Pardocchi, D., Pati, A., Pedretti, G., Pisani, A., Plomaritis, P., Privitera, A. C., Pumpo, R., Quatraro, F., Raimondo, D., Rivellini, G., Rizzo Giovanni, L., Romano, M., Salerno, R., Savarino, E., Scarpulla, G., Sinagra, E., Soncini, M., Tammaro, G., Trovato, C., Vassallo, R., Vinti, M., and Virgilio, C. M. P.

15. Emerging from gastroesophageal reflux (Emerge): An Italian survey - I the viewpoint of the gastroenterologist

Author

Bianchetti, M., Peralta, S., Nicita, R., Salvatore Emanuele Aragona, Ciprandi, G., Arrigoni, A., Artuso, D., Astegiano, M., Azzinnari, C., Battaglia, E., Belcari, C., Bendia, E., Benedicenti, P., Brandimarte, G., Buoncompagni, I., Cabras, M., Camilleri, S., Capece, G., Caronna, S., Cassieri, C., Castaldo, F., Catalano, T., Citarella, C., D’amore, F., D’alia, G., D’arpa, F., Dattola, A., Bortoli, N., Medici, A., Di Marzo, S., Di Mitri, R., Di Napoli, A., D’onofrio, V., Dughera, L., Elisei, W., Errico, G., Familiari, L., Familiari, P., Frasca, R., Frunzio, A., Gatti, M., Genova, S., Grosso, S., Gullotta, R., Iannuzziello, D. R., Indennitate, G., Leonardi, G., Luigiano, C., Macchiarella, B., Maisto, T., Mancino, M. G., Mancino, A., Manes, G., Marin, R., Mastinu, G. R., Moi, A., Montalbano, L. M., Monterosso, N., Morabito Loprete, A., Morlando, L., Ogliari, C., Paiano Primaldo, N., Paliani, G., Palieri, A. P., Pardocchi, D., Pati, A., Pedretti, G., Pisani, A., Plomaritis, P., Privitera, A. C., Pumpo, R., Quatraro, F., Raimondo, D., Rivellini, G., Rizzo Giovanni, L., Romano, M., Salerno, R., Savarino, E., Scarpulla, G., Sinagra, E., Soncini, M., Tammaro, G., Trovato, C., Vassallo, R., Vinti, M., and Virgilio, C. M. P.

16. Estrogens stimulate the proliferation of human cholangiocarcinoma by inducing the expression and secretion of vascular endothelial growth factor.

Author

Mancino A, Mancino MG, Glaser SS, Alpini G, Bolognese A, Izzo L, Francis H, Onori P, Franchitto A, Ginanni-Corradini S, Gaudio E, and Alvaro D

Subjects
Aged, Bile Duct Neoplasms pathology, Bile Ducts, Intrahepatic drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Cholangiocarcinoma pathology, Female, Humans, Male, Receptors, Vascular Endothelial Growth Factor drug effects, Receptors, Vascular Endothelial Growth Factor metabolism, Vascular Endothelial Growth Factor A metabolism, Bile Duct Neoplasms physiopathology, Bile Ducts, Intrahepatic physiopathology, Cholangiocarcinoma physiopathology, Estradiol pharmacology, Estrogens pharmacology, Vascular Endothelial Growth Factor A drug effects
Abstract

Background: Estrogens may induce the proliferation of neoplastic cells by activating neo-angiogenesis., Aim: To evaluate the effect of estrogens on the expression of vascular endothelial growth factor (VEGF) and related receptors (VEGF-R) in human cholangiocarcinoma and the role played by VEGF in mediating the proliferative effects of estrogens., Methods: Seven biopsies of intra-hepatic cholangiocarcinoma and the HuH-28 cell lines were investigated. Cell proliferation was measured by both PCNA Western blot and MTS proliferation assay., Results: By immunohistochemistry, biopsies of human cholangiocarcinoma stained positively for VEGF-A and VEGF-C and related receptors. HuH-28 cells expressed VEGF-A, -C, and VEGFR-1, -2, -3 and, their protein level was enhanced by 17beta-estradiol in association with the stimulation of cell proliferation. 17beta-Estradiol-stimulated proliferation of HuH-28 cells was blocked by 70% by VEGF-TRAP, a receptor-based VEGF inhibitor. 17beta-Estradiol induced the secretion of VEGF in the supernatant of HuH-28 cells. The stimulatory effect of 17beta-estradiol on the protein expression of VEGF-A, VEGF-C and VEGFR-1, -2, -3 was blocked by antagonists of ER (Ici182,780) or insulin-like growth factor 1-receptor (alphaIR3)., Conclusions: With the limitations of experiments performed in a cell line, our study indicates that VEGF plays a major role in mediating the proliferative effects of estrogens on human cholangiocarcinoma.

Published
2009
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17. Bile salts regulate proliferation and apoptosis of liver cells by modulating the IGF1 system.

Author

Drudi Metalli V, Mancino MG, Mancino A, Torrice A, Gatto M, Attili AF, Alpini G, and Alvaro D

Subjects
Animals, Bile Ducts cytology, Bile Ducts metabolism, Hepatocytes metabolism, Male, Rats, Rats, Wistar, Receptor, IGF Type 1 metabolism, Receptors, Estrogen metabolism, Apoptosis physiology, Bile Acids and Salts metabolism, Cell Proliferation, Hepatocytes physiology, Insulin-Like Growth Factor I metabolism
Abstract

Background: In different cell types, the insulin-like growth factor 1 and its receptor modulate growth, apoptosis and damage repair in cooperation with estrogen receptors., Aim: To evaluate the involvement of the insulin-like growth factor 1 system and estrogen receptors in bile salts modulation of apoptosis/proliferation of hepatocytes and cholangiocytes. Primary cultures of rat hepatocytes and cholangiocytes were exposed to glycochenodeoxycholate or tauro-CDC in the presence or absence of insulin-like growth factor 1 receptor blocking antibody (alphaIR3), small interfering RNA for insulin-like growth factor 1, 17beta-estradiol or estrogen receptor antagonist (ICI 182,780). Proliferation was evaluated by proliferating cell nuclear antigen Western blot and apoptosis by measuring caspase-3 activity or annexin-V., Results: In hepatocytes, the insulin-like growth factor 1 receptor blocker enhanced glycochenodeoxycholate-induced apoptosis and caused tauro-CDC to promote apoptosis. 17Beta-estradiol or the estrogen receptor antagonist (ICI 182,780) did not influence the apoptotic effect of glycochenodeoxycholate. In cholangiocytes, both glycochenodeoxycholate and tauro-CDC induced proliferation at 100microM, while they induced apoptosis at 1mM with a more pronounced effect of glycochenodeoxycholate. Apoptosis induced by 1mM glycochenodeoxycholate or tauro-CDC in cholangiocytes was enhanced by blocking insulin-like growth factor 1 receptor or by silencing insulin-like growth factor 1. 17Beta-estradiol counteracts glycochenodeoxycholate-induced cholangiocyte apoptosis by enhancing insulin-like growth factor 1 secretion and activating the insulin-like growth factor 1 system., Conclusions: Modulation of the IGF1 system could represent a potential strategy for the management of bile salts-induced liver injury.

Published
2007
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