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2. Correction: Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network.

Author

Sun, Hong, Harrington, Chelsea, Gerloff, Nancy, Mandelbaum, Mark, Jeffries-Miles, Stacey, Apostol, Lea Necitas G., Valencia, Ma. Anne-Lesley D., Shaukat, Shahzad, Angez, Mehar, Sharma, Deepa K., Nalavade, Uma P., Pawar, Shailesh D., Simbu, Elisabeth Pukuta, Andriamamonjy, Seta, Razafindratsimandresy, Richter, and Vega, Everardo

Published
2024
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5. Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network

Author

Sun, Hong, primary, Harrington, Chelsea, additional, Gerloff, Nancy, additional, Mandelbaum, Mark, additional, Jeffries-Miles, Stacey, additional, Apostol, Lea Necitas G., additional, Valencia, Ma. Anne-Lesley D., additional, Shaukat, Shahzad, additional, Angez, Mehar, additional, Sharma, Deepa K., additional, Nalavade, Uma P., additional, Pawar, Shailesh D., additional, Pukuta Simbu, Elisabeth, additional, Andriamamonjy, Seta, additional, Razafindratsimandresy, Richter, additional, and Vega, Everardo, additional

Published
2021
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6. Diagnostic Assay Development for Poliovirus Eradication

Author

Gerloff, Nancy, primary, Sun, Hong, additional, Mandelbaum, Mark, additional, Maher, Chelsea, additional, Nix, W. Allan, additional, Zaidi, Sohail, additional, Shaukat, Shahzad, additional, Seakamela, Lerato, additional, Nalavade, Uma P., additional, Sharma, Deepa K., additional, Oberste, M. Steven, additional, and Vega, Everardo, additional

Published
2018
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8. A Report From Headquarters.

Author

Mandelbaum, Mark

Subjects
PERIODICALS, ASSOCIATIONS, institutions, etc.
Abstract

Provides information on the 'Journal of the Association for Computing Machinery (ACM).' History of the copyediting process of the periodical; List of staff members at the Publications Department at ACM Headquarters; Web usage and downloads of articles.

Published
2003
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15. Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues

Author

Kilpatrick, David R., primary, Yang, Chen-Fu, additional, Ching, Karen, additional, Vincent, Annelet, additional, Iber, Jane, additional, Campagnoli, Ray, additional, Mandelbaum, Mark, additional, De, Lina, additional, Yang, Su-Ju, additional, Nix, Allan, additional, and Kew, Olen M., additional

Published
2009
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19. Diagnostic Assay Development for Poliovirus Eradication

Author

Gerloff, Nancy, Sun, Hong, Mandelbaum, Mark, Maher, Chelsea, Nix, W. Allan, Zaidi, Sohail, Shaukat, Shahzad, Seakamela, Lerato, Nalavade, Uma P., Sharma, Deepa K., Oberste, M. Steven, and Vega, Everardo

Abstract

ABSTRACTWith poliovirus eradication nearing, few pockets of active wild poliovirus (WPV) transmission remain in the world. Intratypic differentiation (ITD) plays a crucial part in laboratory surveillance as the molecular detection method that can identify and distinguish wild and vaccine-like polioviruses isolated from acute flaccid paralysis cases or environmental sources. The need to detect new variants of WPV serotype 1 (WPV1) and the containment of all serotype 2 polioviruses (PV2) in 2015 required changes to the previous version of the method. The ITD version 5.0 is a set of six real-time reverse transcription-PCR (rRT-PCR) assays that serve as accurate diagnostic tools to easily detect and differentiate PV serotypes and genotypes. We describe the creation and properties of quantitation standards, including 16 control RNA transcripts and nine plaque-isolated viruses. All ITD rRT-PCR assays were validated using these standards, and the limits of detection were determined for each assay. We designed and pilot tested two new assays targeting recently circulating WPV1 genotypes and all PV2 viruses. The WPV1 assay had 99.1% specificity and 100% sensitivity, and the PV2 assay had 97.7% specificity and 92% sensitivity. Before proceeding to the next step in the global poliovirus eradication program, we needed to gain a better understanding of the performance of the ITD 5.0 suite of molecular assays and their limits of detection and specificities. The findings and conclusions in this evaluation serve as building blocks for future development work.

Published
2017
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22. ACS321 EDITORIAL POLICY COORDINATION COMMITTEE AND A301 EDITORIAL AFFAIRS, POLICIES, AND PRACTICES COMMITTEE.

Author

Mandelbaum, Mark

Abstract

Information about topics discussed at the American Society of Agronomy's (ASA) ACS321 Editorial Policy Coordination Committee and A301 Editorial Affairs, Policies and Practices Committee meeting held in San Antonio, Texas on November 18, 2011 is presented. Topics include database publishing, manuscript handling and the committee's goal to help the journals published more themed issues. ASA editor-in-chief Warren Dick reviewed the draft which calls for a stipend of 40 dollars per paper submitted.

Published
2012

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