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Background and Aim: Breast cancer is the most frequent malignancy in women. The consumption of phytochemical components from plants may play an essential role in preventing and treating this cancer. This study aimed to investigate the anti-cancer activity of an ethanolic extract of red okra pods (EEROP) in rats (Rattus norvegicus) induced by N-methyl-N-nitrosourea (MNU). Materials and Methods: The experimental animals were divided into six groups (n=5/group), namely, KN (normal control, without any treatment), K– (negative control, exposed to MNU without EEROP), K+ (positive control, exposed to MNU and Methotrexate), and the treatment Groups P1, P2, and P3 (exposed to MNU and EEROP at doses of 50, 100, and 200 mg/kg body weight [BW], respectively). Intraperitoneal delivery of MNU and EEROP oral administration was carried out for 8 weeks. After the end of treatment, the parameters of cytokines, CD4+ and CD8+ T cells, and mammary gland histology were measured. Results: The results showed that EEROP at doses of 100 and 200 mg/kg BW significantly downregulated interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, IL-17, IL-10, and tumor growth factor-β (p

Background and Aim: Water pollution caused by industrial waste and human activities has disrupted the reproductive health of aquatic organisms. This study aimed to analyze the effects of water pollution caused by polystyrene particles (PP) on the steroid (estradiol and testosterone) levels and histopathology of male tilapia gonads. In addition, we also analyzed the potential of supplementary feeding to remove and neutralize oxidants. Materials and Methods: Thirty-six tilapia fishes were taken for the study and were divided into 12 groups (n=3), including a control group (fed with commercial pellets only) and groups fed with a mixture of commercial-probiotic pellets (200 mL/kg, 1×108 colony-forming unit [CFU]/mL) and commercial vitamin C pellets (100 mg/kg), respectively. The PP concentrations used for this study were 0, 0.1, 1, and 10 mg/L, and the treatment time was 2 weeks. The testosterone and estradiol concentrations were analyzed by enzyme-linked immunosorbent assay and histopathological analysis of the gonads. Results: Laboratory analysis performed using tilapia fishes showed that exposure to a PP concentration of

Background and Aim: Industrial waste, such as heavy metals, is a major source of water pollution; at high levels, such pollution can reduce river water quality to the extent that it becomes unsuitable for aquaculture of freshwater fish. This study aimed to focus on the effects of copper (Cu) exposure in Nile tilapia (Oreochromis niloticus) and specifically the ability of Lactobacillus-based probiotics supplementation in fish feed to ameliorate damage to gonads and negative effects on red blood cells (RBCs), whole blood cells (WBCs), hematocrit (HCT) levels, hemoglobin (HGB) levels, and malondialdehyde (MDA) levels following such exposure. Materials and Methods: Thirty-two Nile tilapia fish were divided into eight groups: A negative control (without probiotics or Cu), a positive control (with probiotics but without Cu), three treatments with probiotics in feed, and one of three Cu concentrations (0.75, 1.50, or 3.00 mg/L), and three treatments with these three Cu concentrations but without probiotics in feed. The probiotics concentration in feed was 25 mL/kg (1×108 CFU/mL). Feeding was for 15 days, after which the hematological parameters, gonadal (testis) structure, and MDA levels of fish were analyzed. Results: Exposure to Cu significantly (p

Background and Aim: For years, people have used sodium nitrite as a food preservative. This study determined the effect of okra (Abelmoschus esculentus L.) pod methanol extract (OPME) on mice with hepatotoxicity induced by sodium nitrite. The flavonoid and total phenolic levels, serum biochemistry, and liver histology were examined. Materials and Methods: Green okra pod extraction was performed using ethanol methanol solvent. Thirty adult male BALB/c mice (8-10 weeks, ∼30 g) were divided into six groups: Normal control, negative control (sodium nitrite 50 mg/kg BW exposure), and treatment groups (sodium nitrite exposure and OPME at doses of 50, 100, 200, and 400 mg/kg BW). Subsequently, they were exposed to sodium nitrite and administered multiple doses of OPME for 19 days by gavage. After that, serum was used for biochemical evaluation, and liver histological analysis was performed. All data were statistically analyzed (α=0.05). Results: All doses of OPME reduced the levels of nitric oxide (NO), malondialdehyde (MDA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). In this research, both superoxide dismutase (SOD) and catalase (CAT) levels increased in all OPME-administered treatments . All doses also reduced necrotic cells, proportion of swollen cells, and inflammation in liver histological analysis. The results of this study showed that OPME exerted hepatoprotective effects by lowering MDA, NO, ALT, and AST levels. It also improved SOD and CAT levels and recovered damaged liver tissue to its normal state. The optimal dose of OPME was 50-100 mg/kg BW. Conclusion: OPME has potential as a natural hepatoprotective agent against sodium nitrite exposure.

Background and Aim: For many years, people use natural products from the plant and fungal to improve immune response against microorganism. This study aimed to investigate the immunomodulatory properties of polysaccharides (PS) from Coriolus versicolor in mice infected by intracellular bacteria Neisseria gonorrhoeae. Materials and Methods: Thirty-six female BALB/C mice were divided into six groups: Normal control, negative control, positive control, P1 (PS before infection), P2 (PS after infection), and P3 (PS before and after infection). PS were administrated for 10 days. N. gonorrhoeae was infected twice with 2 weeks gap from the first to second exposure with a dose of 106 cells. 1 week after the end of treatment, level of oxidants, innate immune responses, and adaptive immune responses were measured. Results: This study showed that PS administration could restore the number of leukocytes as normal but could not enhance the number of phagocytes and its activity. PS administration also showed immunosuppression activity by lowering nitric oxide levels in P2 and P3 groups (p0.05). Conclusion: PS enhance adaptive immunity due to the capability of N. gonorrhoeae that able to survive and replicate in phagocytes. Thus, PS from C. versicolor could be potentially be used as a natural immunomodulator against intracellular bacteria.

Mercury is a widespread aquatic pollutant that adversely affects the reproductive system of male fish. Although the effects of mercuric chloride (HgCl2) on the testicular structure, the sperm count and altered sperm morphology of fish have been investigated, the effects on sperm quality and fertilization are unknown. Therefore, the aim of this study was to investigate the effects of HgCl2 exposure in different concentrations (0; 0.5; 1; 2.5; 5 ppm) on sperm parameters and fertility of male Cyprinus carpio. Sperm quality parameters, including mass and individual time of sperm motility (s), and viability (%) were measured using digital inverted microscopy. The fertilization (%) was measured by counting the number of fertilized eggs. Integrity or DNA fragmentation (%) was measured by Acridine orange test (AOT) using fluorescence microscopy. The change of sperm membrane surface was examined using scanning electron microscopy (SEM). The results showed that the in vitro exposure of mercury could significantly decrease some parameters of fish sperm qualities, i.e. decreased the time for mass and individual motility, viability, DNA fragmentation, and fertilization ability (P

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1), 12-17.

Okra pods were widely consumed by Indonesians to maintain health. The aim of this study was at investigating the potential of crude polysaccharides from okra pods on immune response in mice infected with Staphylococcus aureus. Thirty male Balb/C mice were divided into six groups: normal control, negative control, and treatment groups (administration of crude polysaccharides at doses of 25, 50, 75, and 100 mg/kg). Crude polysaccharides were administrated for fourteen days. Furthermore, mice were exposed to S. aureus at the fifteenth day. Two weeks after the end of treatment, the parameters were measured. This study showed that crude polysaccharides at a dose of 75 and 100 mg/kg improved phagocytic activity, spleen index, and splenocytes proliferation. Rising of TNF-α levels was shown in groups treated with crude polysaccharides at doses of 25, 50, and 100 mg/kg. All treatment groups showed a decreasing level of IL-17. Crude okra polysaccharides also showed a slight increase in NK cells activity and IFN-γ level. Thus, crude okra polysaccharides could act as an effective material to enhance immune response including phagocytic activity, spleen index, splenocytes proliferation, and control immune responses through cytokine production.