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30 results on '"Mao, Shihui"'

9. m6A‐dependent upregulation of DDX21 by super‐enhancer‐driven IGF2BP2 and IGF2BP3 facilitates progression of acute myeloid leukaemia.

Author

Zhao, Yanchun, Zhou, Yutong, Qian, Yu, Wei, Wenwen, Lin, Xiangjie, Mao, Shihui, Sun, Jie, and Jin, Jie

Subjects
ACUTE myeloid leukemia, MYELOID differentiation factor 88, GENE expression, RNA methylation, CELL cycle, INHIBITION of cellular proliferation, SUBSTANCE abuse relapse
Abstract

Background: Acute myeloid leukaemia (AML) is a haematological malignancy with unfavourable prognosis. Despite the effectiveness of chemotherapy and targeted therapy, relapse or drug resistance remains a major threat to AML patients. N6‐methyladenosine (m6A) RNA methylation and super‐enhancers (SEs) are extensively involved in the leukaemogenesis of AML. However, the potential relationship between m6A and SEs in AML has not been elaborated. Methods: Chromatin immunoprecipitation (ChIP) sequencing data from Gene Expression Omnibus (GEO) cohort were analysed to search SE‐related genes. The mechanisms of m6 A‐binding proteins IGF2BP2 and IGF2BP3 on DDX21 were explored via methylated RNA immunoprecipitation (MeRIP) assays, RNA immunoprecipitation (RIP) assays and luciferase reporter assays. Then we elucidated the roles of DDX21 in AML through functional assays in vitro and in vivo. Finally, co‐immunoprecipitation (Co‐IP) assays, RNA sequencing and ChIP assays were performed to investigate the downstream mechanisms of DDX21. Results: We identified two SE‐associated transcripts IGF2BP2 and IGF2BP3 in AML. High enrichment of H3K27ac, H3K4me1 and BRD4 was observed in IGF2BP2 and IGF2BP3, whose expression were driven by SE machinery. Then IGF2BP2 and IGF2BP3 enhanced the stability of DDX21 mRNA in an m6A‐dependent manner. DDX21 was highly expressed in AML patients, which indicated a poor survival. Functionally, knockdown of DDX21 inhibited cell proliferation, promoted cell apoptosis and led to cell cycle arrest. Mechanistically, DDX21 recruited transcription factor YBX1 to cooperatively trigger ULK1 expression. Moreover, silencing of ULK1 could reverse the promoting effects of DDX21 overexpression in AML cells. Conclusions: Dysregulation of SE‐IGF2BP2/IGF2BP3‐DDX21 axis facilitated the progression of AML. Our findings provide new insights into the link between SEs and m6A modification, elucidate the regulatory mechanisms of IGF2BP2 and IGF2BP3 on DDX21, and reveal the underlying roles of DDX21 in AML. [ABSTRACT FROM AUTHOR]

Published
2024
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10. circSLC25A13 acts as a ceRNA to regulate AML progression via miR‐616‐3p/ADCY2 axis

Author

Wei, Wenwen, primary, Pan, Jiajia, additional, Wang, Jinghan, additional, Mao, Shihui, additional, Qian, Yu, additional, Lin, Xiangjie, additional, Ling, Qing, additional, Ye, Wenle, additional, Zhou, Yutong, additional, Zhao, Yanchun, additional, Huang, Jiansong, additional, Huang, Xin, additional, Ma, Zhixin, additional, Wang, Huanping, additional, Li, Chenying, additional, Sun, Jie, additional, and Jin, Jie, additional

Published
2023
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13. Glutamate dehydrogenase 1: A novel metabolic target in inhibiting acute myeloid leukaemia progression

Author

Ma, Zhixin, primary, Ye, Wenle, additional, Wang, Jinghan, additional, Huang, Xin, additional, Huang, Jiansong, additional, Li, Xia, additional, Hu, Chao, additional, Li, Chenying, additional, Zhou, Yile, additional, Lin, Xiangjie, additional, Wei, Wenwen, additional, Qian, Yu, additional, Zhou, Yutong, additional, Mao, Shihui, additional, Yin, Xiufeng, additional, Zhu, Bo, additional, and Jin, Jie, additional

Published
2023
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14. ACC010 , a novel BRD4 inhibitor, synergized with homoharringtonine in acute myeloid leukemia with FLT3‐ ITD

Author

Qian, Yu, primary, Zhang, Xiang, additional, Mao, Shihui, additional, Wei, Wenwen, additional, Lin, Xiangjie, additional, Ling, Qing, additional, Ye, Wenle, additional, Li, Fenglin, additional, Pan, Jiajia, additional, Zhou, Yutong, additional, Zhao, Yanchun, additional, Huang, Xin, additional, Huang, Jiansong, additional, Tong, Hongyan, additional, Sun, Jie, additional, and Jin, Jie, additional

Published
2023
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15. Dihydropyrimidinase‐like 2 can serve as a novel therapeutic target and prognostic biomarker in acute myeloid leukemia

Author

Li, Fenglin, primary, Ling, Qing, additional, Lian, Jiaying, additional, Chen, Ying, additional, Hu, Chao, additional, Yang, Min, additional, Zhang, Xiang, additional, Li, Chenying, additional, Mao, Shihui, additional, Ye, Wenle, additional, Li, Xia, additional, Lin, Xiangjie, additional, Wei, Wenwen, additional, Huang, Xin, additional, Pan, Jiajia, additional, Qian, Yu, additional, Wang, Jinghan, additional, Lu, Ying, additional, and Jin, Jie, additional

Published
2023
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18. Additional file 2 of Homoharringtonine is synergistically lethal with BCL-2 inhibitor APG-2575 in acute myeloid leukemia

Author

Wei, Wenwen, Huang, Shujuan, Ling, Qing, Mao, Shihui, Qian, Yu, Ye, Wenle, Li, Fenglin, Pan, Jiajia, Lin, Xiangjie, Huang, Jiansong, Huang, Xin, Zhai, Yifan, Sun, Jie, and Jin, Jie

Abstract

Additional file 2: Table S1. IC50 values of APG-2575 and ABT-199 against AML cell lines. Table S2. The CI and q values of AML cell lines. Table S3. The combination index values of AML primary patients. Table S4. The evaluation indicators of tumor bearing mice in MV4-11 model. Table S5. The evaluation indicators of tumor bearing mice in OCI-AML3 model.

Published
2022
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20. Global, regional, and national burdens of leukemia from 1990 to 2017: a systematic analysis of the global burden of disease 2017 study

Author

Lin, Xiangjie, primary, Wang, Jinghan, additional, Huang, Xin, additional, Wang, Huafeng, additional, Li, Fenglin, additional, Ye, Wenle, additional, Huang, Shujuan, additional, Pan, Jiajia, additional, Ling, Qing, additional, Wei, Wenwen, additional, Mao, Shihui, additional, Qian, Yu, additional, Jin, Jie, additional, and Huang, Jiansong, additional

Published
2021
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21. Inhibition of CPT1a as a Prognostic Marker can Synergistically Enhance the Antileukemic Activity of ABT199

Author

mao, shihui, primary, Ling, Qing, additional, Pan, Jiajia, additional, Li, Fenglin, additional, Huang, Shujuan, additional, Ye, Wenle, additional, Wei, Wenwen, additional, Lin, Xiangjie, additional, Qian, Yu, additional, Wang, Yungui, additional, Huang, Xin, additional, Huang, Jiansong, additional, Wang, Jinghan, additional, and Jin, Jie, additional

Published
2021
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23. Ibrutinib Suppresses Early Megakaryopoiesis but Enhances Proplatelet Formation

Author

Huang, Jiansong, primary, Huang, Shujuan, primary, Ma, Zhixin, additional, Lin, Xiangjie, additional, Li, Xia, additional, Huang, Xin, additional, Wang, Jinghan, additional, Ye, Wenle, additional, Li, Yang, additional, He, Daqiang, additional, Yang, Min, additional, Pan, Jiajia, additional, Ling, Qing, additional, Li, Fenglin, additional, Mao, Shihui, additional, Wang, Huafeng, additional, Wang, Yungui, additional, and Jin, Jie, additional

Published
2020
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25. MAP4K1 Functions as a Tumor Promotor and Drug Mediator for AML via Modulation of DNA Damage/Repair System and MAPK Pathway

Author

Ling, Qing, primary, Li, Fenglin, additional, Zhang, Xiang, additional, Mao, Shihui, additional, Lin, Xiangjie, additional, Pan, Jiajia, additional, Ye, Wenle, additional, Wei, Wenwen, additional, Qian, Yu, additional, Hu, Chao, additional, Huang, Xin, additional, Wang, Jinghan, additional, Wang, Huafeng, additional, Huang, Jiansong, additional, Wang, Yungui, additional, and Jin, Jie, additional

Published
2020
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27. A Novel Role for Malate Dehydrogenase 1 in Acute Myeloid Leukemia Progression

Author

Huang, Shujuan, primary, Li, Fenglin, additional, Li, Xia, additional, Pan, Jiajia, additional, Mao, Liping, additional, Zhang, Yi, additional, Xu, Yu, additional, Yang, Min, additional, Qian, Jiejing, additional, Yu, Wenjuan, additional, Ling, Qing, additional, Ye, Wenle, additional, Mao, Shihui, additional, Huang, Xin, additional, Wang, Yungui, additional, Li, Ling, additional, Wang, Jinghan, additional, and Jin, Jie, additional

Published
2019
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29. High Expression of BCAT1promotes Acute Myeloid Leukemia Progression and Sensitize to PARP Inhibitor

Author

Jin, Jie, Jin, Jie, Pan, Jiajia, Wang, Yungui, Huang, Shujuan, Mao, Shihui, Ling, Qing, Wang, Jinghan, and Li, Chenying

Abstract

Acute myeloid leukemia (AML) is a highly heterogeneous disease with poor clinical prognosis, especially to cytogenetically normal AML(CN-AML)patients, which belong to moderate prognosis group. The clinical outcomes of them are not consistent. Therefore, further exploration of novel biomarkers and development of new anti-tumor drugs is urgently needed. Recently, accumulating evidence have emerged and demonstrated that branched-chain amino acids (BCAA) are essential for tumor growth and proliferation. Branched-chain aminotransferase 1(BCAT1), a BCAA metabolic enzyme, which correlates with cancer aggression, not only that, it has been reported that overexpression of BCAT1in leukemia cells decreased intracellular αKG levels, showed the characteristics of stem cells and displayed a phenotype like cases carrying IDH mutations. By limiting intracellular αKG, BCAT1high expression AML cells prone to cause an increase in DNA damage probably via suppressing homologous recombination.

Published
2019
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30. LRP8 inhibits bladder cancer cell ferroptosis by activating the Wnt/β-catenin-SCD1 positive feedback loop.

Author

Zhao Y, Shi G, Huang X, Zhang Z, Liao K, Xiong H, Feng Z, Mao S, and Zhang X

Abstract

Background: Advanced bladder cancer (bc) patients often have poor prognoses due to issues such as recurrence and drug resistance. The discovery of ferroptosis has opened new avenues for bc treatment; however, the specific regulatory mechanisms remain to be explored. This study aimed to investigate the mechanisms influencing ferroptosis in bc cells, with a particular focus on the role of low-density lipoprotein receptor-related protein 8 (LRP8)., Methods: We utilized reverse transcription-quantitative polymerase chain reaction and western blot to assess the expression of LRP8 in bc cells, activation of the Wnt/β-catenin signaling pathway, and the expression of genes related to fatty acid synthesis. We measured changes in ferroptosis levels by evaluating mitochondrial membrane potential, Fe2+, malondialdehyde, and reactive oxygen species levels. A xenograft mouse model was employed to validate the impact of LRP8 on bc progression., Results: Cell experiments demonstrated a significant upregulation of LRP8 expression in bc cells. Knockdown of LRP8 induced ferroptosis in bc cells, a process directly triggered by the inhibition of the Wnt/β-catenin signaling pathway. Activation of the Wnt/β-catenin signaling pathway mediated by LRP8 upregulated the expression of stearoyl-CoA desaturase 1 (SCD1), subsequently leading to the suppression of ferroptosis. In vivo experiments indicated that LRP8 knockdown significantly impaired bc growth, accompanied by inhibition of the Wnt/β-catenin-SCD1 axis., Conclusion: LRP8 mediates the synthesis of monounsaturated fatty acids through the Wnt/β-catenin-SCD1 positive feedback loop, thereby inhibiting ferroptosis in bc cells. These findings provide a promising target for the regulation of ferroptosis in bc cells., (© The Author(s) 2025. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)

Published
2025
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