23 results on '"María Teresa Verde"'
Search Results
2. A comparative study of eight serological methods shows that spike protein-based ELISAs are the most accurate tests for serodiagnosing SARS-CoV-2 infections in cats and dogs
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Carlos Diezma-Díaz, Gema Álvarez-García, Javier Regidor-Cerrillo, Guadalupe Miró, Sergio Villanueva-Saz, María Dolores Pérez, María Teresa Verde, Patricia Galán-Malo, Alejandro Brun, Sandra Moreno, Rocío Checa, Ana Montoya, Wesley C. Van Voorhis, and Luis Miguel Ortega-Mora
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SARS-CoV-2 virus ,cat ,dog ,serological tests ,RBD fragment ,nucleocapsid protein ,Veterinary medicine ,SF600-1100 - Abstract
IntroductionCoronavirus disease 2019 (COVID-19) is an infectious zoonotic disease caused by SARS-CoV-2. Monitoring the infection in pets is recommended for human disease surveillance, prevention, and control since the virus can spread from people to animals during close contact. Several diagnostic tests have been adapted from humans to animals, but limited data on the validation process are available.MethodsHerein, the first comparative study of six “in house” and two commercial serological tests developed to monitor SARS-CoV-2 infection in pets was performed with a well-coded panel of sera (61 cat sera and 74 dog sera) with a conservative criterion (viral seroneutralisation and/or RT–qPCR results) as a reference. Four “in house” tests based on either the RBD fragment of the spike protein (RBD-S) or the N-terminal fragment of the nucleoprotein (N) were developed for the first time. The analytical specificity (ASp) of those tests that showed the best diagnostic performance was assessed. The validation included the analysis of a panel of sera obtained pre-pandemic from cats and dogs infected with other coronaviruses to determine the analytical Sp (17 cat sera and 41 dog sera).Results and discussionELISAS based on the S protein are recommended in serosurveillance studies for cats (RBD-S SALUVET ELISA, ELISA COVID UNIZAR and INgezim® COVID 19 S VET) and dogs (INgezim® COVID 19 S VET and RBD-S SALUVET ELISA). These tests showed higher diagnostic sensitivity (Se) and DSp in cats (>90%) than in dogs. When sera obtained prior to the pandemic and from animals infected with other coronaviruses were analyzed by RBD-S and N SALUVET ELISAs and INgezim® COVID 19 S VET, a few cross reactors or no cross reactions were detected when dog and cat sera were analyzed by tests based on the S protein, respectively. In contrast, the number of cross reactions increased when the test was based on the N protein. Thus, the use of tests based on the N protein was discarded for serodiagnosis purposes. The results obtained revealed the most accurate serological tests for each species. Further studies should attempt to improve the diagnostic performance of serological tests developed for dogs.
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- 2023
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3. Anaemia in Sheep Caused by Babesia and Theileria Haemoparasites
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Sergio Villanueva-Saz, Marta Borobia, Antonio Fernández, Calasanz Jiménez, Andrés Yzuel, María Teresa Verde, María Ángeles Ramo, Luis Figueras, and Héctor Ruíz
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Babesia ,lamb ,Theileria ,sheep ,anaemia ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Piroplasmoses in sheep are caused by vector-borne apicomplexan protozoa, Babesia and Theileria. Different species are responsible for the disease; some species are more pathogenic than others and have a worldwide distribution. In this sense, these causative agents can cause anaemia in flocks. In general, these vector-borne diseases infect small ruminants and cause host-mediated pathology. In the case of Babesia species, a combination of different mechanisms is involved: red blood cell lysis due to intracellular parasite multiplication, activation of biogenic amines and the coagulation system with the possibility of disseminated intravascular coagulation. By contrast, less information is available on the different immunopathogenic mechanisms involved in the development of anaemia in sheep with theileriosis. However, the mechanisms of pathogenic action in theileriosis are similar to those studied in babesiosis. Diagnosis is based on compatible clinical signs, laboratory findings, specific diagnostic tests and the presence of the tick vector. Some of these tests detect the causative agent itself, such as direct identification by light microscopy and molecular analysis. In contrast, other tests detect the sheep’s immune response to the organism by serology. Both diseases pose a significant diagnostic challenge for veterinary practitioners around the world. This review presents the most frequent clinical signs, pathogenesis and clinicopathological findings, diagnosis.
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- 2022
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4. Copper Poisoning, a Deadly Hazard for Sheep
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Marta Borobia, Sergio Villanueva-Saz, Marta Ruiz de Arcaute, Antonio Fernández, María Teresa Verde, José María González, Teresa Navarro, Alfredo A. Benito, José Luis Arnal, Marcelo De las Heras, and Aurora Ortín
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chronic copper poisoning ,acute copper poisoning ,copper toxicity ,hepatic copper accumulation ,sheep ,lamb ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Copper (Cu) is an essential microelement for animals. However, sheep are particularly susceptible to Cu intoxication, a deadly disease reported worldwide. The risk of developing this poisoning is higher in vulnerable breeds and in intensively managed lambs or milk sheep. Two types of Cu intoxication can occur depending on the chronic or acute exposure to Cu. In chronic Cu poisoning (CCP), the most common form, Cu is accumulated in the liver during a subclinical period. A low intake of Cu antagonists (molybdenum, sulphur, iron, or zinc) favours Cu accumulation. The sudden release of Cu into the blood causes acute haemolysis with anaemia, haemoglobinuria, jaundice and death within 1–2 days. Acute Cu poisoning is related to the accidental administration or ingestion of toxic amounts of Cu. Acute oral exposure to Cu causes severe gastroenteritis, shock and death. Collapse and death occur shortly after parenteral administration. The diagnosis is based on history, clinical, gross pathological, histological and toxicological findings. Treatment of sheep with severe clinical signs often has poor success but is very effective during the Cu accumulation phase. Different therapies, based on either chelating agents or Cu antagonists, have been used to treat and prevent CCP.
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- 2022
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5. Comparative Study of the Use of Doxycycline and Oxytetracycline to Treat Anaplasmosis in Fattening Lambs
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Delia Lacasta, Héctor Ruiz, Aurora Ortín, Sergio Villanueva-Saz, Agustín Estrada-Peña, José María González, Juan José Ramos, Luis Miguel Ferrer, Alfredo Ángel Benito, Raquel Labanda, Carlos Malo, María Teresa Verde, Antonio Fernández, and Marta Ruiz de Arcaute
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anaplasmosis ,lambs ,Anaplasma ovis ,jaundice ,carcass condemnation ,oxytetracycline ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Lamb icteric carcasses condemnation due to Anaplasma ovis is causing relevant economic losses. A comparative study was developed on the effects of different antibiotics to treat ovine anaplasmosis in fattening lambs. A total of 100 A. ovis naturally infected lambs were selected and randomly divided into four groups of 25 lambs: Group ID, treated with injectable doxycycline; Group OD, oral doxycycline; Group O, injectable oxytetracycline; and Group C, untreated animals for the control group. Clinical, haematological, and molecular analyses were performed before the treatment and 12 and 45 days after the beginning of the treatments, and carcass condemnation was followed after slaughter. The A. ovis bacterial load was high before the treatments in the four groups and decreased significantly 45 days after treatment in the ID and O Groups (p < 0.001). The parameters that were related to haemolysis showed similar results. At the abattoir, 15 out of the 47 examined carcasses were condemned; 7 of C Group, 6 of OD Group, 2 of O Group, and 0 of ID Group. It can be concluded that injectable doxycycline and oxytetracycline significantly reduce A. ovis bacterial load in blood and carcass condemnation at the abattoir. Further studies are needed in order to confirm these encouraging findings.
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- 2022
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6. Comparison of a qualitative immunochromatographic test with two quantitative serological assays for the detection of antibodies to Leishmania infantum in dogs
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Sergio Villanueva-Saz, Asier Basurco, Víctor Martín, Antonio Fernández, Araceli Loste, and María Teresa Verde
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Diagnostic techniques and procedures ,Immunoglobulins ,Canine leishmaniosis ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Canine leishmaniosis is a disease caused by Leishmania infantum, a vector-borne parasite. Due to the zoonotic potential of canine leishmaniosis, infected dogs must be identified. Serological assays are the most common methods for the detection of L. infantum infection in dogs used in veterinary practice. The aim of the study was to assess the performance of a rapid immunochromatographic test (FASTest LEISH®, MEGACOR Diagnostik) for the detection of specific antibodies to that of the L. infantum in dog sera. The results were simultaneously compared using a commercial brand of indirect immunofluorescence antibody test and an in-house enzyme-linked immunosorbent assay as references. Between the two reference tests, 232 serum samples out of 244, produced concordant results while 12 exhibited discordant results. Of the 232 concordant samples, 121 were classified as L. infantum seropositive, and 111 samples were previously classified as L. infantum seronegative by a combination of the reference assays. All samples that were seropositive by the reference tests were also positive according to the rapid test, and only one sample that was seronegative according to the two reference assays was positive according to the rapid test. Compared with the reference tests, the rapid test sensitivity was 100%, specificity was 99.1%, accuracy was 99.6%, Cohen’s kappa coefficient was 0.99, and the area under receiver operating characteristic curve was 0.995. The FASTest LEISH® is a rapid, qualitative in-clinic test with high sensitivity and specificity.
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- 2019
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7. Evaluation of the performance of three serological tests for diagnosis of Leishmania infantum infection in dogs using latent class analysis
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Asier Basurco, Alda Natale, Katia Capello, Antonio Fernández, María Teresa Verde, Ana González, Andrés Yzuel, Jacobo Giner, and Sergio Villanueva-Saz
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Bayesian analysis ,canine leishmaniasis ,diagnostic techniques and procedures ,gold standard ,immunoglobulins ,Animal culture ,SF1-1100 - Abstract
Abstract Canine leishmaniasis (CanL) is a disease caused by Leishmania infantum. Serological methods are the most common diagnostic techniques used for the diagnosis of the CanL. The objective of our study was to estimate the sensitivity and specificity of one in-house ELISA kit (ELISA UNIZAR) and three commercially available serological tests (MEGACOR Diagnostik GmbH) including an immunochromatographic rapid test (FASTest LEISH®), an immunofluorescent antibody test (MegaFLUO LEISH®) and an enzyme-linked immunosorbent assay (MegaELISA LEISH®), using latent class models in a Bayesian analysis. Two hundred fifteen serum samples were included. The highest sensitivity was achieved for FASTest LEISH® (99.38%), ELISA UNIZAR (99.37%), MegaFLUO LEISH® (99.36%) followed by MegaELISA LEISH® (98.49%). The best specificity was obtained by FASTest LEISH® (98.43%), followed by ELISA UNIZAR (97.50%), whilst MegaFLUO LEISH® and MegaELISA LEISH® obtained the lower specificity (91.94% and 91.93%, respectively). The results of present study indicate that the immunochromatographic rapid test evaluated FASTest LEISH® show similar levels of sensitivity and specificity to the quantitative commercial tests. Among quantitative serological tests, sensitivity and specificity were similar considering ELISA or IFAT techniques.
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- 2020
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8. Effect of oral administration of Propionibacterium acnes on growth performance, DTH response and anti-OVA titers in goat kids
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Luis Miguel Ferrer, Antonio Fernández, Araceli Loste, Aurora Ortín, Delia Lacasta, Juan José Ramos, María Teresa Verde, and Tomás Conde
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Propionibacterium acnes ,OVA ,immunostimulant ,goat kids ,immunitary response ,Veterinary medicine ,SF600-1100 - Abstract
Immunostimulants are susbstances that stimuli the response of effector cells to activate the immune response such as antigen uptake, cytokine release or antibody response. These substances can increase resistence to infection by different types of microorganisms, reducing dependence of antibiotics used in livestock animals. Recent reports have demonstrated the positive effect of Propionibacterium acnes (P. acnes) to control animal diseases. In this study, we evaluated the effect of the non-specific immunostimulant P. acnes on immunological functions and growth performance in goat kids. Twenty five goat kids served as control group (A) and another 25 animals received P. acnes being the experimental group (B). Kids were challenged with ovalbumin (OVA) to assess humoral immunity. To assess in vivo cell immunity, delayed type hypersensitivity (DTH) test with phytohemagglutinin (PHA) was used, clinical signs and body weight were recorded each week until 9 weeks of age when the experiment ended. Blood samples were obtained to analyze serum proteins fractions and anti-OVA specific antibodies. No clinical signs of disease and no differences (p>0.05) on body weight between groups were recorded (7.32±0.81 kg in group A, 7.13±0.65 kg in group B). Goat kids from group B had more total protein (59.8±5g/l) and albumin levels (32.8±3.3g/l) than goat kids from group A (56.6±5.7 g/l, 29.6±3.9 g/l respectively) (p
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- 2013
9. Evaluation of an immunochromatographic serologic test to detect the presence of anti‐ Toxoplasma gondii antibodies in cats
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Sergio Villanueva‐Saz, Mariví Martínez, Jacobo Giner, María Dolores Pérez, Ana Pilar Tobajas, Andrés Yzuel, María Teresa Verde, Delia Lacasta, Antonio Fernández, Diana Marteles, and Héctor Ruíz
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General Veterinary - Published
- 2023
10. Assessing cortisol concentration in different matrices: predictive potential and relationship with production levels, lactation stage and parity in dairy buffaloes
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Alessio Cotticelli, Giovanna Bifulco, Isabella Pividori, Roberta Matera, Maria Teresa Verde, Matteo Santinello, Alberto Prandi, and Tanja Peric
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italian mediterranean buffalo ,radioimmunoassay ,days in milk ,cortisol concentration ,mature equivalent milk yield ,Animal culture ,SF1-1100 - Abstract
Cortisol is the primary biomarker associated with the hypothalamic–pituitary–adrenal (HPA) axis. This research aimed at assessing the predictive potential and the relationship of cortisol concentrations in four media (blood, milk, whey and hair) with parity, lactation stage and productive classes. Multiparous (n = 30) and primiparous (n = 38) Italian Mediterranean buffaloes were used and assigned to four productive classes (based on percentiles of mature equivalent milk yield (EMY) and mature equivalent energy corrected milk (eECM)), and cortisol concentrations were measured using a in house radioimmunoassay (RIA) method. Parity did not show a significant effect on cortisol concentrations of the four media. The catabolic stage of lactation (up to 90 d in milk (DIM)) was characterised by higher cortisol concentrations compared to the second anabolic stage (beyond 150 DIM) in milk formulations. The plasmatic concentrations of cortisol were higher at the catabolic and the first anabolic stage (91–150 DIM) compared to the second anabolic (p = 0.022 and p = 0.009, respectively). Buffaloes beyond 150 DIM differed from those below 90 DIM (p
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- 2024
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11. Comparison of circulating CD4+, CD8+ lymphocytes and cytokine profiles between dogs with atopic dermatitis and healthy dogs
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María Teresa Verde, Sergio Villanueva-Saz, Araceli Loste, Diana Marteles, Desirée Pereboom, Tomás Conde, and Antonio Fernández
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General Veterinary - Abstract
Introduction Canine atopic dermatitis (cAD) is an inflammatory skin disease characterized by impaired immune function. Changes in the proportions of CD4+ and CD8+ lymphocytes and the serum concentrations of cytokines in the pathogenesis of cAD have been described. Objectives To assess whether the changes in the ratio of CD4+ and CD8+ lymphocytes in the peripheral blood of atopic dogs at the time of diagnosis are related to the severity of the disease. Furthermore, we determined whether the changes in the serum concentrations of the cytokines IL-31, IL-34, IL-10, IFN-γ, and TNF-α were different between atopic and control dogs. Procedures Fifty-six client-owned dogs with atopic dermatitis and 53 healthy control dogs were used. The percentages of CD4+ and CD8+ lymphocytes were determined by imaging flow cytometry. The index of CADESI-03 was calculated. Serum cytokine levels were analyzed using ELISA. Results Atopic dogs showed a higher percentage of CD8+ lymphocytes, a lower CD4+/CD8+ ratio than healthy dogs, and a positive correlation with CADESI-03. Atopic dogs also showed higher serum IL-31 and IL-34 levels and lower IL-10 levels. A moderate positive correlation was found between serum IL-31 and CADESI-03. Conclusions The CD4+/CD8+ ratio may be a sensitive parameter that positively correlates with the severity of cAD, and elevated serum levels of IL-31 and IL-34 may facilitate diagnosis of the disease.
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- 2022
12. Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both
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María Teresa Verde, Antonio Rezusta, Carmen Aspiroz, Montserrat Pérez, Yolanda Gilaberte, P. Robres, Rosa Bolea, Vanesa Pérez-Laguna, and Bernardino Moreno
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Medicine (General) ,medicine.medical_specialty ,photoinactivation ,medicine.medical_treatment ,Scars ,wound healing ,Mupirocin ,Photodynamic therapy ,Skin infection ,medicine.disease_cause ,chemistry.chemical_compound ,R5-920 ,Antibiotic resistance ,Medicine ,S aureus ,superficial wound infection ,Original Research ,mupirocin ,integumentary system ,business.industry ,General Medicine ,SKH-1 mice ,medicine.disease ,Antimicrobial ,Dermatology ,chemistry ,Staphylococcus aureus ,antimicrobial ,lipids (amino acids, peptides, and proteins) ,medicine.symptom ,business ,Wound healing - Abstract
Background: Antibiotic resistance and impaired wound healing are major concerns in S. aureus superficial skin infections, and new therapies are needed. Antimicrobial photodynamic therapy (aPDT) is a new therapeutic approach for infections, but it also improves healing in many wound models.Objective: To compare the antimicrobial activity and the effects on wound healing of aPDT based on Methylene Blue (MB-aPDT) with mupirocin treatment, either alone or in combination, in superficial skin wounds of S. aureus-infected mice. Additionally, to evaluate the clinical, microbiological, and cosmetic effects on wound healing.Materials and Methods: A superficial skin infection model of S. aureus was established in SKH-1 mice. Infected wounds were treated with MB-aPDT, MB-aPDT with a daily topical mupirocin or only with mupirocin. No treatment was carried out in control animals. Daily clinical and microbiological examinations were performed until complete clinical wound healing. Histopathological studies and statistical analysis were performed at the end of the study.Results: MB-aPDT treatment induced the best wound healing compared to mupirocin alone or to mupirocin plus MB-aPDT. Superficial contraction at 24 h and a greater reduction in size at 48 h, quicker detachment of the crust, less scaling, and absence of scars were observed. Histopathological studies correlated with clinical and gross findings. By contrast, mupirocin showed the highest logaritmic reduction of S. aureus.Conclusions: MB-aPDT and mupirocin treatments are effective in a murine superficial skin infection model of S. aureus. One session of MB-aPDT was the best option for clinical wound healing and cosmetic results. The addition of mupirocin to MB-aPDT treatment improved antimicrobial activity; however, it did not enhance wound healing. No synergistic antibacterial effects were detected.
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- 2021
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13. Comparison of a qualitative immunochromatographic test with two quantitative serological assays for the detection of antibodies to Leishmania infantum in dogs
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Víctor S. Martín, A. Loste, Sergio Villanueva-Saz, Asier Basurco, Antonio Fernández, and María Teresa Verde
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Canine leishmaniosis ,040301 veterinary sciences ,Immunochromatographic test ,Antibodies, Protozoan ,Immunoglobulins ,Enzyme-Linked Immunosorbent Assay ,Test sensitivity ,Brief Communication ,Sensitivity and Specificity ,Serology ,0403 veterinary science ,Dogs ,parasitic diseases ,Diagnostic techniques and procedures ,Animals ,Medicine ,Dog Diseases ,Leishmania infantum ,Fluorescent Antibody Technique, Indirect ,Immunoassay ,lcsh:Veterinary medicine ,General Veterinary ,Receiver operating characteristic ,biology ,business.industry ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,biology.organism_classification ,Serum samples ,040201 dairy & animal science ,Virology ,Specific antibody ,biology.protein ,Leishmaniasis, Visceral ,lcsh:SF600-1100 ,Antibody ,business - Abstract
Canine leishmaniosis is a disease caused by Leishmania infantum, a vector-borne parasite. Due to the zoonotic potential of canine leishmaniosis, infected dogs must be identified. Serological assays are the most common methods for the detection of L. infantum infection in dogs used in veterinary practice. The aim of the study was to assess the performance of a rapid immunochromatographic test (FASTest LEISH®, MEGACOR Diagnostik) for the detection of specific antibodies to that of the L. infantum in dog sera. The results were simultaneously compared using a commercial brand of indirect immunofluorescence antibody test and an in-house enzyme-linked immunosorbent assay as references. Between the two reference tests, 232 serum samples out of 244, produced concordant results while 12 exhibited discordant results. Of the 232 concordant samples, 121 were classified as L. infantum seropositive, and 111 samples were previously classified as L. infantum seronegative by a combination of the reference assays. All samples that were seropositive by the reference tests were also positive according to the rapid test, and only one sample that was seronegative according to the two reference assays was positive according to the rapid test. Compared with the reference tests, the rapid test sensitivity was 100%, specificity was 99.1%, accuracy was 99.6%, Cohen’s kappa coefficient was 0.99, and the area under receiver operating characteristic curve was 0.995. The FASTest LEISH® is a rapid, qualitative in-clinic test with high sensitivity and specificity. Electronic supplementary material The online version of this article (10.1186/s13028-019-0473-1) contains supplementary material, which is available to authorized users.
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- 2019
14. Antibodies to Leishmania infantum in peritoneal effusion from a dog with atypical signs of leishmaniosis: treatment and follow‐up
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María Teresa Verde, Cristina Peréz Sala, Antonio Fernández, Sergio Villanueva-Saz, and Andrés Yzuel Estevez
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Pathology ,medicine.medical_specialty ,040301 veterinary sciences ,biology.animal_breed ,French bulldog ,Peritoneal Effusion ,0403 veterinary science ,parasitic diseases ,Medicine ,General Veterinary ,biology ,medicine.diagnostic_test ,business.industry ,Peritoneal fluid ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Abdominal distension ,Jaundice ,biology.organism_classification ,medicine.disease ,040201 dairy & animal science ,Pancytopenia ,Abdominal ultrasonography ,medicine.symptom ,Leishmania infantum ,business - Abstract
A three-year-old entire male French bulldog was brought in with the following clinical signs: apathy, vomiting and abdominal distension. Physical examination showed dehydration, tachypnoea and presence of jaundice. Abdominal radiology revealed the presence of an important loss of details in the abdominal structures compatible with peritoneal effusion, confirmed by abdominal ultrasonography. Findings detected by ultrasonography consisted of splenomegaly with alteration of the echotexture, hepatomegaly with presence of nodules and irregular areas in the liver capsule. An ultrasound-guided sample of the peritoneal fluid was taken for laboratory analysis. Abnormal clinicopathological results included pancytopenia, hyperglobulinaemia, hypoalbuminaemia, and elevation of the hepatic parameters and total bilirubin. High antibodies to Leishmania infantum were detected by ELISA in serum and peritoneal fluid samples. However, PCR for L infantum showed a negative result from the peritoneal fluid sample. This is the first reported case of canine leishmaniosis with the specific detection of L infantum antibodies in peritoneal effusion.
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- 2020
15. Incorporación del urianálisis como técnica laboratorial en el abordaje diagnóstico de casos clinicos (ABP) para la adquisición de competencias profesionales
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María Teresa Verde Arribas, Cristina Albisu, and Laura Navarro Combalía
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Innovación educativa ,Aprendizaje basado en problemas ,Veterinary Medicine ,Last courses ,Laboratorio ,Problem-based learning ,Skills ,Competencias ,Urinalysis ,Últimos cursos ,Urianálisis ,Laboratory ,Clinical cases ,Educación superior ,Enseñanza superior ,Tecnologías y educación ,Veterinaria ,Casos clínicos - Abstract
[EN] Problem-based learning (PBL) is one of the most used methodologies in the teaching of medical disciplines such as veterinary medicine, since it allows applying the knowledge acquired in a real context. In addition, urinalysis is a laboratory technique essential in the veterinary clinical practice for the diagnostic approach of clinical cases. Therefore, we set out to carry out an experience with two objectives: 1) to promote the acquisition of skills in the laboratory, especially in urinalysis, and 2) to promote the acquisition of competencies by integrating the results of urinalysis in the diagnostic approach of a real case. The experience was carried out with students of the last courses of the veterinary degree at the Veterinary Hospital of Zaragoza. The students assessed various aspects of the proposed objectives through a survey. 47 students participated, valuing the experience as very satisfactory. The majority perceived to have increased their skills in the laboratory by performing the urinalysis technique, and considered as very useful the integration of urinalysis results in the diagnostic approach of the cases, considering in general, that the knowledge acquired could be very useful in their professional future., [ES] El aprendizaje basado en problemas (ABP) es una de las metodologías más empleadas en la docencia de disciplinas biomédicas como la medicina interna veterinaria, ya que permite afianzar el aprendizaje de una forma muy sólida aplicando los conocimientos previos adquiridos en materias más básicas a un contexto real. Permite a los estudiantes poner en valor el conocimiento adquirido previamente y da sentido a los planteamientos académicos, que, de otra forma, no acaban de ser entendidos por los propios estudiantes. Por otro lado, el urianálisis es una técnica laboratorial imprescindible en la práctica clínica veterinaria para el abordaje diagnóstico de todos los casos clínicos relacionados con enfermedades del sistema urinario, pero que, además, coplementa la valoración de la situación global de cualquier proceso orgánico. Por ello, nos propusimos realizar una experiencia con dos objetivos: 1) fomentar la adquisición de habilidades en el laboratorio, especialmente en el urianálisis y 2) fomentar la adquisición de competencias integrando los resultados del urianálisis en el abordaje diagnóstico de un caso real. La experiencia se llevó a cabo con alumnos/as de últimos cursos del grado de Veterinaria en el Hospital Veterinario de la Universidad de Zaragoza. El alumnado evaluó, mediante una encuesta, distintos aspectos sobre los objetivos planteados. 47 alumnos/as participaron valorando la experiencia como muy satisfactoria. La mayoría percibió haber aumentado sus destrezas en el laboratorio mediante la realización de la técnica de urianálisis, y valoró como muy útil la integración de los resultados del urianálisis en el abordaje diagnóstico de los casos, considerando en general, que los conocimientos adquiridos podrían serles muy útiles en su futuro profesional y poniendo mucho mas en valor los conocimientos que poseían de la materia relacionada de cursos precedentes, lo que se traduce en un incremento de su autoestima al hacerse conscientes de la proyección real que pueden tener los conocimientos adquiridos.
- Published
- 2019
16. Treatment and follow-up of a domestic ferret (Mustela putorius furo) with clinical leishmaniosis caused by Leishmania infantum
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Caterina Fani, María Teresa Verde, M. Magdalena Alcover, Roser Fisa, Antonio Fernández, Jacobo Giner, Sergio Villanueva-Saz, Andrés Yzuel, Cristina Riera, Asier Basurco, and Michele Trotta
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0301 basic medicine ,Allopurinol ,Meglumine antimoniate ,030231 tropical medicine ,Physiology ,Urine ,Serology ,03 medical and health sciences ,0302 clinical medicine ,Leishmaniosi ,parasitic diseases ,Crystalluria ,medicine ,Animals ,Leishmania infantum ,Leishmaniasis ,Communicable diseases in animals ,Meglumine Antimoniate ,Antiparasitic Agents ,General Veterinary ,biology ,Ferrets ,Parasitologia veterinària ,030108 mycology & parasitology ,biology.organism_classification ,Trypanocidal Agents ,Cryptosporidium parvum ,Mustela putorius ,Leishmaniasis, Visceral ,Drug Therapy, Combination ,Female ,Veterinary parasitology ,Parasitology ,medicine.symptom ,Malalties infeccioses en els animals ,medicine.drug - Abstract
Leishmania infantum infection including treatment and follow up in domestic animals other than dogs and cats has not been described at this moment. This article describes the anti-Leishmania treatment and follow-up of a ferret (Mustela putorius furo) with leishmaniosis. A combined therapeutic protocol established for the patient, not yet approved for ferrets, was a combination of meglumine antimoniate plus allopurinol. A follow-up was established monthly during the first year in order to monitor the health condition of the patient. Six months after commencing allopurinol therapy, xanthine crystalluria was observed in urine sediment with no other urine alterations detected by urine analysis. The ferret worsened progressively with diarrhoea and weight loss after cohabiting with another ferret diagnosed with cryptosporidiosis. Cryptosporidium parvum was isolated in faecal samples from the patient detected by three different methods including Ziehl-Neelsen staining, a qualitative test to detection of C. parvum antigens and finally a specific molecular analysis to characterize the species. To the best of the authors´ knowledge, this is the first report providing information about anti-Leishmania protocol therapy used and follow-up in a domestic ferret with clinical leishmaniosis. Veterinarians practicing in endemic areas should be aware of this infection in ferrets at risk and their susceptibility especially when immunosuppressive conditions are present.
- Published
- 2020
17. Validation of a radioimmunoassay method for cortisol in buffalo milk whey. A preparatory step for future sensor technology
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Alessio Cotticelli, Maria Teresa Verde, Roberta Matera, Isabella Pividori, Alberto Prandi, Gianluca Neglia, and Tanja Peric
- Subjects
allostatic load ,stress ,cortisol ,animal welfare ,buffalo milk ,Animal culture ,SF1-1100 - Abstract
One animal-based method to evaluate welfare is the presence of stress. In dairy ruminants, the responses to stressors include the activation of both neuroendocrine and autonomous nervous system that can be evaluated through an endocrine assessment. The present study aimed to validate a radioimmunoassay method for cortisol in buffalo milk. Three formulations (whole and skimmed milk and whey) and three solvents (methanol diethyl ether and dichloromethane) were tested: methanol was characterised by the best extraction efficiency (69.88%), whey cortisol concentrations showed a significant correlation with whole extracted milk and were not affected by fat content variation during the milking session. The RIA used in the present study showed good precision, sensitivity and specificity: the dilutions test indicated the high reproducibility of the results, overlapping of the dilution curve and standard curve highlighted high specificity and the lack of interfering factors by buffalo whey matrix. It is concluded that the present assay suits the cortisol measurement in buffalo milk and the ranges described could be employed in the calibration of a biosensing technologies directly integrated in milking parlour systems.Highlights Buffalo milk whey revealed to be a matrix of great interest because of its high stability in terms of storage, transportation and processing. RIA method suits the cortisol measurement in buffalo milk Ranges described can be employed in the calibration of biosensors for non-invasive assessment of cortisol
- Published
- 2022
- Full Text
- View/download PDF
18. Effects of allergen-specific immunotherapy on peripheral blood regulatory T cells and serum concentrations of cytokines and immunoglobulins in horses with allergic dermatitis
- Author
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Desirée Pereboom, Antonio Fernández, Alvaro Casanova, A. Ortín, Tomás Conde, Sergio Villanueva-Saz, Diana Marteles, and María Teresa Verde
- Subjects
Male ,0301 basic medicine ,medicine.medical_treatment ,T cell ,Immunology ,T-Lymphocytes, Regulatory ,Dermatitis, Atopic ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Immunology and Allergy ,Allergic dermatitis ,Horses ,IL-2 receptor ,Pharmacology ,biology ,business.industry ,Immunotherapy ,Immunoglobulin E ,Serum concentration ,Peripheral blood ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,Desensitization, Immunologic ,Immunoglobulin G ,030220 oncology & carcinogenesis ,biology.protein ,Cytokines ,Female ,Horse Diseases ,Antibody ,business - Abstract
The aim of this study was to assess the effect of allergen-specific immunotherapy (ASIT) on the immunological responses of horses. Blood samples were taken from thirty-two horses with allergic dermatitis treated with ASIT and 10 healthy control horses at 0, 3, 6, 9 and 12 months to investigate the evolution of the percentage of regulatory T cells (Treg) in the peripheral blood and the serum levels of cytokines and immunoglobulins. Clinical improvement was appreciated by the majority of the horses' owners (56.6%). No effect of ASIT on CD4+CD25High Treg cells was found during the one year treatment period. No differences in the percentage of CD4+ T cells were observed between the groups, and no effects of ASIT over time were observed. The percentage of CD25+ T cells was always higher in the ASIT group (17.9 ± 11.3%) than in the control group (7.3 ± 4.4%, p < 0.001). We did not detect any effect of ASIT on the serum levels of TGF-β, IL-10 and IFN-γ or on the serum concentrations of IgA and IgG4. A reduction in the serum levels of total IgE in the horses with allergic dermatitis was observed at the 6th month (p < 0.05), but increased again at the end of the study. The results indicate that immunotherapy was insufficient to induce significant changes that could indicate T cell tolerance, a shift in cytokine production to more protective Th1 cells. More studies are needed with new vaccine compositions and administration protocols to improve the immunological responses of the horses with allergic dermatitis.
- Published
- 2019
19. Effect of oral administration of Propionibacterium acnes on growth performance, DTH response and anti-OVA titers in goat kids
- Author
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Delia Lacasta, Antonio Fernández, María Teresa Verde, Tomás Conde, Juan José Ramos, Luis Miguel Ferrer, A. Ortín, and A. Loste
- Subjects
Goat Diseases ,lcsh:Veterinary medicine ,General Veterinary ,biology ,immunitary response ,medicine.drug_class ,goat kids ,biology.organism_classification ,Blood proteins ,Group A ,Immunostimulant ,Group B ,Propionibacterium acnes ,Immune system ,OVA ,Immunology ,Humoral immunity ,medicine ,lcsh:SF600-1100 ,immunostimulant - Abstract
Immunostimulants are susbstances that stimuli the response of effector cells to activate the immune response such as antigen uptake, cytokine release or antibody response. These substances can increase resistence to infection by different types of microorganisms, reducing dependence of antibiotics used in livestock animals. Recent reports have demonstrated the positive effect of Propionibacterium acnes (P. acnes) to control animal diseases. In this study, we evaluated the effect of the non-specific immunostimulant P. acnes on immunological functions and growth performance in goat kids. Twenty five goat kids served as control group (A) and another 25 animals received P. acnes being the experimental group (B). Kids were challenged with ovalbumin (OVA) to assess humoral immunity. To assess in vivo cell immunity, delayed type hypersensitivity (DTH) test with phytohemagglutinin (PHA) was used, clinical signs and body weight were recorded each week until 9 weeks of age when the experiment ended. Blood samples were obtained to analyze serum proteins fractions and anti-OVA specific antibodies. No clinical signs of disease and no differences (p>0.05) on body weight between groups were recorded (7.32±0.81 kg in group A, 7.13±0.65 kg in group B). Goat kids from group B had more total protein (59.8±5g/l) and albumin levels (32.8±3.3g/l) than goat kids from group A (56.6±5.7 g/l, 29.6±3.9 g/l respectively) (p
- Published
- 2013
20. Influence of lairage time on some welfare and meat quality parameters in pigs
- Author
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María Pilar Zaragoza, María Pilar Pérez, Jorge H. Calvo, G. Chacón, Manuel Gascón, Jorge Palacio, María Teresa Verde, María Pilar Santolaria, Sylvia García-Belenguer, and María del Carmen Aceña
- Subjects
Blood Glucose ,Male ,Waiting time ,Meat ,Time Factors ,Genotype ,Hydrocortisone ,General Veterinary ,Swine ,Longissimus Thoracis ,Large white ,Hydrogen-Ion Concentration ,Biology ,Animal Welfare ,Reflectivity ,Animal science ,Stress, Physiological ,Lactates ,Animals ,Female ,Food Deprivation ,Muscle, Skeletal ,Abattoirs - Abstract
A total of 150 Large White cross Landrace pigs (110-120 kg) of both sexes were used to investigate the effects of three different lairage times (0 h, 3 h, 9 h). Blood samples were collected at exanguination and cortisol, glucose, lactate, muscle enzymes and haematological parameters were determined. Post-mortem measurements of muscle pH were taken at 20 min, 2 h and 24 h from Longissimus thoracis and Semimembranosus. Lairage time showed a significant effect on pH24, internal muscle reflectance using the fibre optic probe (FOP24), red blood cells, neutrophils and lymphocytes, glucose and enzymatic activities. Changes in blood profile and meat quality parameters indicated that three hours of lairage in Spanish commercial conditions may reduce the amount of stress exhibited by pigs at slaughter and better meat quality can be obtained. No lairage or an excessively long lairage period without food may compromise animal welfare and meat quality.
- Published
- 2002
21. Cutaneous adverse reaction to furosemide treatment: new clinical findings
- Author
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Pablo Gómez, Ochoa, María Teresa Verde, Arribas, Javier Miana, Mena, and Manuel Gascón, Pérez
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Heart Failure ,Male ,Dogs ,integumentary system ,Furosemide ,Pruritus ,Animals ,Alopecia ,Scientific ,Dog Diseases ,skin and connective tissue diseases ,Diuretics - Abstract
A poodle was admitted for investigation of pruritus and a lesional pattern of erythema and alopecia located in the dorsolumbar area. After differential diagnosis ruling out several processes a rare side effect to furosemide, not yet described in canine medicine was confirmed as the possible causative agent.
- Published
- 2006
22. Variations of clinical biochemical parameters of laying hens and broiler chickens fed aflatoxin-containing feed
- Author
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Antonio Fernández, Juan José Ramos, J. Gomez, María Teresa Verde, G. Chavez, D. F. Luco, and Manuel Gascón
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chemistry.chemical_compound ,Aflatoxin ,Animal science ,General Immunology and Microbiology ,Food Animals ,Triglyceride ,chemistry ,Calcium concentration ,Broiler ,Animal Science and Zoology ,Fatty infiltration ,Biology - Abstract
Two groups of 32 laying hens (Hyssex Brown) and two groups of 32 23-day-old (Hybro) broiler chickens were fed 2.5 and 5 parts/10(6) of aflatoxin in their diet for 4, 8, 16 and 32 days; 16 hens and 32 chicks were maintained as control groups (0 parts/10(6)). After the intoxication period, a clearance period was established of 1, 2, 4 and 8 days. Relative weights of liver and kidneys significantly increased in intoxicated hens, but not in broiler chickens. Histological lesions in both types of bird consisted of hepatic cell vacuolation with fatty infiltration. There was a significant decrease (P0.001) in egg production in the 5 parts/10(6) group, which started to recover during the clearance period. No morbidity or mortality due to the aflatoxicosis were observed in either type of bird. In intoxicated laying hens, cholesterol levels were not significantly (P0.05) different from control values, but triglyceride levels decreased (P0.001) in both intoxicated groups. The effect of aflatoxin on calcium and phosphorus levels was important, because on the 4th day their values decreased significantly. Aspartate aminotransferase (AST) serum levels remained normal, whereas alanino aminotransferase (ALT) activity decreased in both intoxicated groups. The activity of serum lactic dehydrogenase (LDH) and gammaglutamil transferase (GGT) increased significantly. In intoxicated broiler chickens, aflatoxins did not alter (P0.05) the biochemical parameters studied, except that the serum calcium concentration was lower in the 5 parts/10(6) group. These data indicated that in intoxicated laying hens, a severe clinical biochemical alteration was produced, and that this together with the hepatic lesions observed in hens and broilers may aid disease diagnosis.
- Published
- 1994
23. Cutaneous adverse reaction to furosemide treatment: new clinical findings.
- Author
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Ochoa PG, Arribas MT, Mena JM, and Pérez MG
- Subjects
- Alopecia chemically induced, Alopecia etiology, Alopecia veterinary, Animals, Diuretics therapeutic use, Dog Diseases chemically induced, Dogs, Furosemide therapeutic use, Heart Failure drug therapy, Heart Failure veterinary, Male, Pruritus chemically induced, Pruritus etiology, Diuretics adverse effects, Dog Diseases etiology, Furosemide adverse effects, Pruritus veterinary
- Abstract
A poodle was admitted for investigation of pruritus and a lesional pattern of erythema and alopecia located in the dorsolumbar area. After differential diagnosis ruling out several processes a rare side effect to furosemide, not yet described in canine medicine was confirmed as the possible causative agent.
- Published
- 2006
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