92 results on '"Marc S. Wold"'
Search Results
2. Gain-of-Function Mutations in RPA1 Cause a Syndrome with Short Telomeres and Somatic Genetic Rescue
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Masayoshi Honda, Melchior Lauten, Marcus B. Valentine, Patrick Revy, Stéphane Coulon, Richa Sharma, Charnise Goodings, Caroline Kannengiesser, Fabian Beier, Melanie Boerries, Shondra M. Pruett-Miller, Sophie L Granger, Miriam Erlacher, Maria Spies, Axel Künstner, Megan A. Cooper, Jill A. Rosenfeld, Vincent Géli, Carole Saintomé, Victor B Pastor, Charlotte M. Niemeyer, Dmitri Churikov, Marcin W. Wlodarski, Sophia Polychronopoulou, Hauke Busch, Ti-Cheng Chang, Sandrine Hirschi, Louis Sanchez, Charikleia Kelaidi, Sushree S. Sahoo, Marc S. Wold, Alfonso G Fernandez, Sarah K. Nicholas, Indian Institute of Technology Delhi (IIT Delhi), Uppsala University, Freiburg Institute for Advanced Studies-LifeNet, Albert-Ludwigs-Universität Freiburg, St Jude Children's Research Hospital, University of Iowa [Iowa City], Sorbonne Université (SU), Universität zu Lübeck = University of Lübeck [Lübeck], Universitätsklinikum RWTH Aachen - University Hospital Aachen [Aachen, Germany] (UKA), Rheinisch-Westfälische Technische Hochschule Aachen University (RWTH), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), CHU Strasbourg, Université de Strasbourg (UNISTRA), German Cancer Consortium [Heidelberg] (DKTK), University Hospital Schleswig-Holstein-Campus Luebeck, 'Aghia Sophia' Children's Hospital, University of Freiburg [Freiburg], Washington University in Saint Louis (WUSTL), Baylor College of Medicine (BCM), Baylor University, AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Muséum national d'Histoire naturelle (MNHN), German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Structure et Instabilité des Génomes (STRING), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University Medical Center of Schleswig–Holstein = Universitätsklinikum Schleswig-Holstein (UKSH), Kiel University, Molecular Genetics of Breast Cancer, German Cancer Research Center (DKFZ), Washington University School of Medicine in St. Louis, Physiopathologie et Epidémiologie des Maladies Respiratoires (PHERE (UMR_S_1152 / U1152)), Sorbonne Université - UFR Sciences de la vie (UFR 927 ), Universität zu Lübeck [Lübeck], RWTH Aachen University, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)
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Adult ,Male ,Heterozygote ,Adolescent ,Somatic cell ,[SDV]Life Sciences [q-bio] ,Immunology ,[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,Biology ,medicine.disease_cause ,Biochemistry ,Germline ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Replication Protein A ,medicine ,Missense mutation ,Humans ,Child ,Gene ,Telomere Shortening ,030304 developmental biology ,Genetics ,0303 health sciences ,Mutation ,Infant, Newborn ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology ,Cell Differentiation ,Cell Biology ,Hematology ,DNA-binding domain ,Bone Marrow Failure Disorders ,Middle Aged ,Telomere ,3. Good health ,[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics ,[SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology ,Gain of Function Mutation ,Myelodysplastic Syndromes ,Female ,Blood Commentary ,Stem cell ,030215 immunology - Abstract
Human telomere biology disorders (TBD)/short telomere syndromes (STS) are heterogeneous disorders caused by inherited loss-of-function mutations in telomere-associated genes. Here, we identify 3 germline heterozygous missense variants in the RPA1 gene in 4 unrelated probands presenting with short telomeres and varying clinical features of TBD/STS, including bone marrow failure, myelodysplastic syndrome, T- and B-cell lymphopenia, pulmonary fibrosis, or skin manifestations. All variants cluster to DNA-binding domain A of RPA1 protein. RPA1 is a single-strand DNA-binding protein required for DNA replication and repair and involved in telomere maintenance. We showed that RPA1E240K and RPA1V227A proteins exhibit increased binding to single-strand and telomeric DNA, implying a gain in DNA-binding function, whereas RPA1T270A has binding properties similar to wild-type protein. To study the mutational effect in a cellular system, CRISPR/Cas9 was used to knock-in the RPA1E240K mutation into healthy inducible pluripotent stem cells. This resulted in severe telomere shortening and impaired hematopoietic differentiation. Furthermore, in patients with RPA1E240K, we discovered somatic genetic rescue in hematopoietic cells due to an acquired truncating cis RPA1 mutation or a uniparental isodisomy 17p with loss of mutant allele, coinciding with stabilized blood counts. Using single-cell sequencing, the 2 somatic genetic rescue events were proven to be independently acquired in hematopoietic stem cells. In summary, we describe the first human disease caused by germline RPA1 variants in individuals with TBD/STS.
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- 2021
3. A minimal threshold of FANCJ helicase activity is required for its response to replication stress or double-strand break repair
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Sanjay Kumar Bharti, Lynda Bradley, Robert M. Brosh, Joshua A. Sommers, Keir C. Neuman, Irfan Khan, Sanket Awate, Marina A. Bellani, Kazuo Shin-ya, Graeme A. King, Koji Kobayashi, Yuliang Wu, Dana Branzei, Marc S. Wold, Takuye Abe, Yeonee Seol, Hiroyuki Kitao, and Venkatasubramanian Vidhyasagar
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0301 basic medicine ,Aphidicolin ,DNA Replication ,DNA Repair ,DNA damage ,DNA repair ,Mutation, Missense ,DNA, Single-Stranded ,Cell Line ,Recombinases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Stress, Physiological ,Replication Protein A ,Genetics ,Animals ,DNA Breaks, Double-Stranded ,Replication protein A ,Oxazoles ,Adenosine Triphosphatases ,biology ,Nucleic Acid Enzymes ,DNA replication ,DNA Helicases ,Helicase ,Processivity ,Fanconi Anemia Complementation Group Proteins ,Cell biology ,G-Quadruplexes ,030104 developmental biology ,Fanconi Anemia ,chemistry ,030220 oncology & carcinogenesis ,Checkpoint Kinase 1 ,biology.protein ,DNA Polymerase Inhibitor ,Rad51 Recombinase ,Cisplatin ,Chickens ,RNA Helicases - Abstract
Fanconi Anemia (FA) is characterized by bone marrow failure, congenital abnormalities, and cancer. Of over 20 FA-linked genes, FANCJ uniquely encodes a DNA helicase and mutations are also associated with breast and ovarian cancer. fancj−/− cells are sensitive to DNA interstrand cross-linking (ICL) and replication fork stalling drugs. We delineated the molecular defects of two FA patient-derived FANCJ helicase domain mutations. FANCJ-R707C was compromised in dimerization and helicase processivity, whereas DNA unwinding by FANCJ-H396D was barely detectable. DNA binding and ATP hydrolysis was defective for both FANCJ-R707C and FANCJ-H396D, the latter showing greater reduction. Expression of FANCJ-R707C or FANCJ-H396D in fancj−/− cells failed to rescue cisplatin or mitomycin sensitivity. Live-cell imaging demonstrated a significantly compromised recruitment of FANCJ-R707C to laser-induced DNA damage. However, FANCJ-R707C expressed in fancj-/- cells conferred resistance to the DNA polymerase inhibitor aphidicolin, G-quadruplex ligand telomestatin, or DNA strand-breaker bleomycin, whereas FANCJ-H396D failed. Thus, a minimal threshold of FANCJ catalytic activity is required to overcome replication stress induced by aphidicolin or telomestatin, or to repair bleomycin-induced DNA breakage. These findings have implications for therapeutic strategies relying on DNA cross-link sensitivity or heightened replication stress characteristic of cancer cells.
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- 2018
4. RPA1 Gain of Function Causes Human Short Telomere Syndrome with Revertant Somatic Mosaicism
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Melchior Lauten, Victor B Pastor, Miriam Erlacher, Masayoshi Honda, Richa Sharma, Marc S. Wold, Melanie Börries, Sushree S. Sahoo, Charnise Goodings-Harris, Charlotte M. Niemeyer, Marcin W. Wlodarski, Maria Spies, Hauke Busch, and Fabian Beier
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Genetics ,Telomerase ,Mutation ,Immunology ,Mutant ,Cell Biology ,Hematology ,Biology ,medicine.disease ,medicine.disease_cause ,Biochemistry ,Germline ,Telomere ,medicine ,Replication protein A ,Dyskeratosis congenita ,Exome sequencing - Abstract
Dyskeratosis congenita (DC) is a short telomere syndrome with bone marrow failure (BMF), mucocutaneous fragility and predisposition to malignancy due to inherited mutations in telomere associated genes. The genetic cause remains unknown in 10-20% of DC patients. Here, we describe a new DC candidate gene, RPA1, which encodes the largest subunit of the Replication Protein A (RPA) complex, a single stranded DNA (ssDNA) binding protein essential for DNA replication, damage repair and telomere maintenance. The patient presented at the age of 5 years with pancytopenia and hypocellular marrow with morphology resembling refractory cytopenia of childhood, mucocutaneous triad and a congenital retinal anomaly. Further workup revealed telomeres in blood below 1st percentile, normal female karyotype and a negative chromosomal breakage test. After excluding known genetic causes for DC, family whole exome sequencing identified a de novoRPA1 c.718G>A (p.E240K) mutation with a predicted pathogenic CADD score of 23 and absent in >140,000 gnomAD population controls. The mutation had 50% allelic frequency in fibroblasts in comparison to a reduced mutation burden of 27% in bone marrow (BM) cells. Using bulk and single cell sequencing of BM, we identified 2 somatic compensatory events that likely explain patient's stable hematologic phenotype over a period of 20 years: i) an acquired RPA1 p.E579X mutation (in cis with p.E240K) was found at 10% frequency, which resulted in RNA degradation of the mutated allele as confirmed by RNA sequencing; ii) a founder clone with uniparental isodisomy on chromosome 17p encompassing RPA1 locus causing loss of germline E240K mutation. Analysis of serial BM samples using SNP arrays and deep sequencing demonstrated UPD17p clonal expansion concurrent with a decline of germline p.E240K and increase of acquired p.E579X mutation. This is consistent with a toxic, likely gain-of-function (GOF) effect of germline p.E240K mutation leading to acquired loss-of-function (LOF) escape mechanisms. RPA1 is essential to all DNA metabolism transactions that encounter ssDNA, including binding to telomeric 3' overhangs during late S-phase to unfold G-quadruplexes and facilitate telomerase activity. Several RPA1 genetic variants impart telomere shortening and an S-phase defect in yeast and human cell lines. However, the molecular mechanisms are not well understood and thus far, RPA1 gene has not been linked to human disease. In an iPSC-based disease model with homozygous RPA1 p.E240K knock-in, we discovered severe telomere shortening in RPA1 mutant iPSCs and iPSC derived hematopoietic progenitors (HPs) compared to WT counterparts. Furthermore, decreased erythroid differentiation capacity was noted in RPA1-mutant HPs. Because the germline p.E240K mutation is located in DNA binding domain A of RPA1, we performed electromobility shift assay to assess the DNA binding affinity of mutant RPA1 protein. The p.E240K heterotrimeric RPA complex shows increased ssDNA binding compared to wild type RPA. In summary, we describe the first human disease manifesting as short telomere syndrome secondary to a de novoRPA1 mutation with likely GOF effect, which forces the development of multiple revertant mosaic events, similar to the newly described SAMD9/SAMD9L disorders. Our studies also have implications for the mechanistic understanding of the role of RPA1 in telomere maintenance and hematopoiesis. Disclosures Niemeyer: Celgene: Consultancy; Novartis: Consultancy.
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- 2020
5. RPA Interacts with HIRA and Regulates H3.3 Deposition at Gene Regulatory Elements in Mammalian Cells
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Hui Zhou, Zhiquan Wang, Mats Ljungman, Tamas Ordog, Marc S. Wold, Honglian Zhang, Zhiguo Zhang, Haiyun Gan, and Jeong Heon Lee
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0301 basic medicine ,Transcription, Genetic ,Cell Cycle Proteins ,Transfection ,complex mixtures ,Article ,Histones ,03 medical and health sciences ,Transcription (biology) ,Replication Protein A ,Humans ,Histone Chaperones ,Protein Interaction Domains and Motifs ,Promoter Regions, Genetic ,Enhancer ,Molecular Biology ,Replication protein A ,Gene ,Regulation of gene expression ,Binding Sites ,biology ,G1 Phase ,Promoter ,DNA ,Cell Biology ,Molecular biology ,Chromatin ,DNA-Binding Proteins ,Enhancer Elements, Genetic ,HEK293 Cells ,030104 developmental biology ,Histone ,biology.protein ,RNA Interference ,HeLa Cells ,Protein Binding ,Transcription Factors - Abstract
The histone chaperone HIRA is involved in depositing histone variant H3.3 into distinct genic regions, including promoters, enhancers, and gene bodies. However, how HIRA deposits H3.3 to these regions remains elusive. Through a short hairpin RNA (shRNA) screening, we identified single-stranded DNA binding protein replication protein A (RPA) as a regulator of the deposition of newly synthesized H3.3 into chromatin. We show that RPA physically interacts with HIRA to form RPA-HIRA-H3.3 complexes, and it co-localizes with HIRA and H3.3 at gene promoters and enhancers. Depletion of RPA1, the largest subunit of the RPA complex, dramatically reduces both HIRA association with chromatin and the deposition of newly synthesized H3.3 at promoters and enhancers and leads to altered transcription at gene promoters. These results support a model whereby RPA, best known for its role in DNA replication and repair, recruits HIRA to promoters and enhancers and regulates deposition of newly synthesized H3.3 to these regulatory elements for gene regulation.
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- 2017
6. A slipped-CAG DNA-binding small molecule induces trinucleotide-repeat contractions in vivo
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Takahiro Otabe, Terence Gall-Duncan, Stella Lanni, Scott Davidson, John Huddleston, Christopher E. Pearson, Hana Tanaka, Jinxing Li, Lisa-Monique Edward, Marc S. Wold, Jean-Yves Masson, Adam Shlien, Masanori P. Takahashi, Kazuhiko Nakatani, Evan E. Eichler, Marietta Y.W.T. Lee, Marie-Christine Caron, Karen Chiang, Jennifer Luo, Xiaoxiao Wang, Hideki Hayakawa, Niraj Joshi, Mehdi Layeghifard, Gagan B. Panigrahi, Mauro Santibanez-Koref, Hideki Mochizuki, Akihiro Sakata, Katherine M. Munson, Richard Gallon, Asako Murata, Masayuki Nakamori, and Tanya Prasolava
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DNA Replication ,Male ,Transcription, Genetic ,Mutant ,Mice, Transgenic ,Biology ,Protein aggregation ,Quinolones ,Medium spiny neuron ,DNA Mismatch Repair ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Ribonucleases ,Transcription (biology) ,Genetics ,Animals ,Humans ,Allele ,Naphthyridines ,030304 developmental biology ,0303 health sciences ,Huntingtin Protein ,DNA replication ,DNA ,TATA-Box Binding Protein ,Corpus Striatum ,Cell biology ,Disease Models, Animal ,Huntington Disease ,chemistry ,Mutation ,Microsatellite Instability ,Trinucleotide repeat expansion ,Trinucleotide Repeat Expansion ,030217 neurology & neurosurgery - Abstract
In many repeat diseases, such as Huntington's disease (HD), ongoing repeat expansions in affected tissues contribute to disease onset, progression and severity. Inducing contractions of expanded repeats by exogenous agents is not yet possible. Traditional approaches would target proteins driving repeat mutations. Here we report a compound, naphthyridine-azaquinolone (NA), that specifically binds slipped-CAG DNA intermediates of expansion mutations, a previously unsuspected target. NA efficiently induces repeat contractions in HD patient cells as well as en masse contractions in medium spiny neurons of HD mouse striatum. Contractions are specific for the expanded allele, independently of DNA replication, require transcription across the coding CTG strand and arise by blocking repair of CAG slip-outs. NA-induced contractions depend on active expansions driven by MutSβ. NA injections in HD mouse striatum reduce mutant HTT protein aggregates, a biomarker of HD pathogenesis and severity. Repeat-structure-specific DNA ligands are a novel avenue to contract expanded repeats.
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- 2018
7. Dynamics and Selective Remodeling of the DNA Binding Domains of RPA
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Maria Spies, Colleen C. Caldwell, Marc S. Wold, Elliot I Corless, Emma A. Tillison, Nina Jocic, Edwin Antony, Nilisha Pokhrel, Joseph Tibbs, and S. M. Ali Tabei
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0303 health sciences ,Chemistry ,DNA damage ,genetic processes ,Dynamics (mechanics) ,RAD52 ,Context (language use) ,DNA-binding domain ,complex mixtures ,3. Good health ,enzymes and coenzymes (carbohydrates) ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Biophysics ,Replication protein A ,030217 neurology & neurosurgery ,Recombination ,DNA ,030304 developmental biology - Abstract
Replication protein A (RPA) coordinates important DNA metabolic events by stabilizing single-strand DNA (ssDNA) intermediates, activating the DNA damage response, and handing off ssDNA to appropriate downstream players. Six DNA binding domains (DBDs) in RPA promote high affinity binding to ssDNA, but also allow RPA displacement by lower affinity proteins. We have made fluorescent versions of RPA and visualized the conformational dynamics of individual DBDs in the context of the full-length protein. We show that both DBD-A and DBD-D rapidly bind to and dissociate from ssDNA, while RPA as a whole remains bound to ssDNA. The recombination mediator protein Rad52 selectively modulates the dynamics of DBD-D. This demonstrates how RPA interacting proteins, with lower ssDNA binding affinity, can access the occluded ssDNA and remodel individual DBDs to replace RPA.One Sentence SummaryThe choreography of binding and rearrangement of the individual domains of RPA during homologous recombination is revealed.
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- 2018
8. Dynamics and selective remodeling of the DNA-binding domains of RPA
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S. M. Ali Tabei, Marc S. Wold, Emma A. Tillison, Colleen C. Caldwell, Joseph Tibbs, Nina Jocic, Nilisha Pokhrel, Maria Spies, Edwin Antony, and Elliot I Corless
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RAD52 ,Saccharomyces cerevisiae ,genetic processes ,DNA, Single-Stranded ,Context (language use) ,Plasma protein binding ,environment and public health ,complex mixtures ,Catechin ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Structural Biology ,Replication Protein A ,Humans ,Molecular Biology ,Replication protein A ,030304 developmental biology ,0303 health sciences ,biology ,DNA-binding domain ,biology.organism_classification ,3. Good health ,Rad52 DNA Repair and Recombination Protein ,enzymes and coenzymes (carbohydrates) ,chemistry ,Biophysics ,health occupations ,030217 neurology & neurosurgery ,DNA ,Recombination ,Protein Binding - Abstract
Replication protein A (RPA) coordinates important DNA metabolic events by stabilizing single-strand DNA (ssDNA) intermediates, activating the DNA damage response and handing off ssDNA to appropriate downstream players. Six DNA binding domains (DBDs) in RPA promote high affinity binding to ssDNA yet also allow RPA displacement by lower affinity proteins. We generated fluorescent versions of Saccharomyces cerevisiae RPA and visualized the conformational dynamics of individual DBDs in the context of the full-length protein. We show that both DBD-A and DBD-D rapidly bind to and dissociate from ssDNA while RPA remains bound to ssDNA. The recombination mediator protein Rad52 selectively modulates the dynamics of DBD-D. These findings reveal how RPA interacting proteins with lower ssDNA binding affinities can access the occluded ssDNA and remodel individual DBDs to replace RPA., One Sentence Summary: The choreography of binding and rearrangement of the individual domains of RPA during homologous recombination is revealed.
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- 2018
9. Single-Molecule Analysis of Replication Protein A-DNA Interactions
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Fletcher E, Bain, Laura A, Fischer, Ran, Chen, and Marc S, Wold
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DNA-Binding Proteins ,Microscopy, Fluorescence ,Staining and Labeling ,Replication Protein A ,Image Processing, Computer-Assisted ,Video Recording ,DNA, Single-Stranded ,Recombinational DNA Repair ,Recombinant Proteins ,Single Molecule Imaging ,Fluorescent Dyes ,Protein Binding - Abstract
Replication protein A (RPA) is a highly conserved, eukaryotic ssDNA-binding protein essential for genome stability. RPA interacts with ssDNA and with protein partners to coordinate DNA replication, repair, and recombination. Single-molecule analysis of RPA-DNA interactions is leading to a better understanding of the molecular interactions and dynamics responsible for RPA function in cells. Here, we first describe how to express, purify, and label RPA. We then describe how to prepare materials and carry out single-molecule experiments examining RPA-DNA interactions using total internal reflection fluorescence microscopy (TIRFM). Finally, the last section describes how to analyze TIRFM data. This chapter will focus on human RPA. However, these methods can be applied to RPA homologs from other species.
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- 2018
10. Single-Molecule Analysis of Replication Protein A–DNA Interactions
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Marc S. Wold, Laura A Fischer, Fletcher E. Bain, and Ran Chen
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0301 basic medicine ,Total internal reflection fluorescence microscope ,030102 biochemistry & molecular biology ,Chemistry ,DNA replication ,Computational biology ,complex mixtures ,enzymes and coenzymes (carbohydrates) ,03 medical and health sciences ,030104 developmental biology ,Biotinylation ,Homologous chromosome ,Molecule ,Replication protein A ,Recombination ,Function (biology) - Abstract
Replication protein A (RPA) is a highly conserved, eukaryotic ssDNA-binding protein essential for genome stability. RPA interacts with ssDNA and with protein partners to coordinate DNA replication, repair, and recombination. Single-molecule analysis of RPA-DNA interactions is leading to a better understanding of the molecular interactions and dynamics responsible for RPA function in cells. Here, we first describe how to express, purify, and label RPA. We then describe how to prepare materials and carry out single-molecule experiments examining RPA-DNA interactions using total internal reflection fluorescence microscopy (TIRFM). Finally, the last section describes how to analyze TIRFM data. This chapter will focus on human RPA. However, these methods can be applied to RPA homologs from other species.
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- 2018
11. Stress-induced acidification may contribute to formation of unusual structures in C9orf72-repeats
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Robert B. Macgregor, Mila Mirceta, Marc S. Wold, Christopher E. Pearson, Rashid Abu-Ghazalah, and Bita Zamiri
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0301 basic medicine ,Circular dichroism ,Biophysics ,Context (language use) ,G-quadruplex ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Cytosine ,0302 clinical medicine ,Stress, Physiological ,Humans ,Molecular Biology ,Gel electrophoresis ,DNA Repeat Expansion ,C9orf72 Protein ,Mutagenesis ,RNA ,Hydrogen-Ion Concentration ,G-Quadruplexes ,030104 developmental biology ,chemistry ,Nucleic Acid Conformation ,Acids ,030217 neurology & neurosurgery ,DNA - Abstract
Background Expansion of the C9orf72 hexanucleotide repeat (GGGGCC)n·(GGCCCC)n is the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Both strands of the C9orf72 repeat have been shown to form unusual DNA and RNA structures that are thought to be involved in mutagenesis and/or pathogenesis. We previously showed that the C-rich DNA strands from the C9orf72 repeat can form four-stranded quadruplexes at neutral pH. The cytosine residues become protonated under slightly acidic pH (pH 4.5–6.2), facilitating the formation of intercalated i-motif structures. Methods Using CD spectroscopy, UV melting, and gel electrophoresis, we demonstrate a pH-induced structural transition of the C-rich DNA strand of the C9orf72 repeat at pHs reported to exist in living cells under stress, including during neurodegeneration and cancer. Results We show that the repeats with lengths of 4, 6, and 8 units, form intercalated quadruplex i-motifs at low pH (pH Conclusions In the proper sequence context, i-motif structures can form at pH values found in some cells in vivo. General significance DNA conformational plasticity exists over broad range of solution conditions.
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- 2017
12. Repair-specific Functions of Replication Protein A
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Koonyee Lam, Cathy S. Hass, and Marc S. Wold
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DNA Replication ,DNA Repair ,DNA repair ,Mutation, Missense ,DNA, Single-Stranded ,Eukaryotic DNA replication ,Saccharomyces cerevisiae ,DNA and Chromosomes ,Biology ,complex mixtures ,Biochemistry ,DNA polymerase delta ,S Phase ,Mice ,Replication factor C ,Control of chromosome duplication ,Replication Protein A ,Animals ,Humans ,Molecular Biology ,Replication protein A ,DNA replication ,Cell Cycle Checkpoints ,Cell Biology ,Molecular biology ,Protein Structure, Tertiary ,Cell biology ,enzymes and coenzymes (carbohydrates) ,Drosophila melanogaster ,Amino Acid Substitution ,Origin recognition complex ,HeLa Cells - Abstract
Replication protein A (RPA), the major eukaryotic single-strand DNA (ssDNA)-binding protein, is essential for replication, repair, recombination, and checkpoint activation. Defects in RPA-associated cellular activities lead to genomic instability, a major factor in the pathogenesis of cancer and other diseases. ssDNA binding activity is primarily mediated by two domains in the 70-kDa subunit of the RPA complex. These ssDNA interactions are mediated by a combination of polar residues and four conserved aromatic residues. Mutation of the aromatic residues causes a modest decrease in binding to long (30-nucleotide) ssDNA fragments but results in checkpoint activation and cell cycle arrest in cells. We have used a combination of biochemical analysis and knockdown replacement studies in cells to determine the contribution of these aromatic residues to RPA function. Cells containing the aromatic residue mutants were able to progress normally through S-phase but were defective in DNA repair. Biochemical characterization revealed that mutation of the aromatic residues severely decreased binding to short ssDNA fragments less than 20 nucleotides long. These data indicate that altered binding of RPA to short ssDNA intermediates causes a defect in DNA repair but not in DNA replication. These studies show that cells require different RPA functions in DNA replication and DNA repair.
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- 2012
13. Molecular Cooperation between the Werner Syndrome Protein and Replication Protein A in Relation to Replication Fork Blockage
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Marc S. Wold, David K. Orren, Guo Min Li, Enerlyn M. Lozada, and Amrita Machwe
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DNA Replication ,Genome instability ,Premature aging ,Aging ,congenital, hereditary, and neonatal diseases and abnormalities ,Werner Syndrome Helicase ,DNA Repair ,Carcinogenesis ,RecQ helicase ,DNA Recombination ,DNA and Chromosomes ,Biology ,DNA Helicase ,complex mixtures ,Biochemistry ,Genomic Instability ,03 medical and health sciences ,Minichromosome maintenance ,Replication Protein A ,medicine ,Humans ,education ,Molecular Biology ,Replication protein A ,030304 developmental biology ,Werner syndrome ,Adenosine Triphosphatases ,0303 health sciences ,education.field_of_study ,RecQ Helicases ,030302 biochemistry & molecular biology ,DNA Helicases ,DNA replication ,nutritional and metabolic diseases ,Cell Biology ,medicine.disease ,Molecular biology ,Cell biology ,enzymes and coenzymes (carbohydrates) ,Exodeoxyribonucleases ,Werner Syndrome ,DNA Damage ,Protein Binding - Abstract
The premature aging and cancer-prone disease Werner syndrome is caused by loss of function of the RecQ helicase family member Werner syndrome protein (WRN). At the cellular level, loss of WRN results in replication abnormalities and chromosomal aberrations, indicating that WRN plays a role in maintenance of genome stability. Consistent with this notion, WRN possesses annealing, exonuclease, and ATPase-dependent helicase activity on DNA substrates, with particularly high affinity for and activity on replication and recombination structures. After certain DNA-damaging treatments, WRN is recruited to sites of blocked replication and co-localizes with the human single-stranded DNA-binding protein replication protein A (RPA). In this study we examined the physical and functional interaction between WRN and RPA specifically in relation to replication fork blockage. Co-immunoprecipitation experiments demonstrated that damaging treatments that block DNA replication substantially increased association between WRN and RPA in vivo, and a direct interaction between purified WRN and RPA was confirmed. Furthermore, we examined the combined action of RPA (unmodified and hyperphosphorylation mimetic) and WRN on model replication fork and gapped duplex substrates designed to bind RPA. Even with RPA bound stoichiometrically to this gap, WRN efficiently catalyzed regression of the fork substrate. Further analysis showed that RPA could be displaced from both substrates by WRN. RPA displacement by WRN was independent of its ATPase- and helicase-dependent remodeling of the fork. Taken together, our results suggest that, upon replication blockage, WRN and RPA functionally interact and cooperate to help properly resolve replication forks and maintain genome stability.
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- 2011
14. Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling
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Jun Hyuk Choi, Marc S. Wold, Michael G. Kemp, Aaron C. Mason, Aziz Sancar, and Laura A. Lindsey-Boltz
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Multidisciplinary ,DNA damage ,DNA replication ,G2-M DNA damage checkpoint ,Biology ,complex mixtures ,Cell biology ,enzymes and coenzymes (carbohydrates) ,chemistry.chemical_compound ,Biochemistry ,chemistry ,CHEK1 ,biological phenomena, cell phenomena, and immunity ,Signal transduction ,Replication protein A ,DNA-PKcs ,DNA - Abstract
ATR kinase is a critical upstream regulator of the checkpoint response to various forms of DNA damage. Previous studies have shown that ATR is recruited via its binding partner ATR-interacting protein (ATRIP) to replication protein A (RPA)-covered single-stranded DNA (RPA-ssDNA) generated at sites of DNA damage where ATR is then activated by TopBP1 to phosphorylate downstream targets including the Chk1 signal transducing kinase. However, this critical feature of the human ATR-initiated DNA damage checkpoint signaling has not been demonstrated in a defined system. Here we describe an in vitro checkpoint system in which RPA-ssDNA and TopBP1 are essential for phosphorylation of Chk1 by the purified ATR-ATRIP complex. Checkpoint defective RPA mutants fail to activate ATR kinase in this system, supporting the conclusion that this system is a faithful representation of the in vivo reaction. Interestingly, we find that an alternative form of RPA (aRPA), which does not support DNA replication, can substitute for the checkpoint function of RPA in vitro, thus revealing a potential role for aRPA in the activation of ATR kinase. We also find that TopBP1 is recruited to RPA-ssDNA in a manner dependent on ATRIP and that the N terminus of TopBP1 is required for efficient recruitment and activation of ATR kinase.
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- 2010
15. A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression
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Troy D. Humphreys, Marc S. Wold, and Stuart J. Haring
- Subjects
DNA re-replication ,DNA Replication ,DNA Repair ,DNA repair ,Molecular Sequence Data ,Eukaryotic DNA replication ,Apoptosis ,Genome Integrity, Repair and Replication ,DNA replication factor CDT1 ,03 medical and health sciences ,0302 clinical medicine ,Replication factor C ,Control of chromosome duplication ,Replication Protein A ,Genetics ,Humans ,Amino Acid Sequence ,Replication protein A ,030304 developmental biology ,0303 health sciences ,biology ,Sequence Homology, Amino Acid ,Cell Cycle ,Genomics ,Molecular biology ,Cell biology ,DNA-Binding Proteins ,Protein Subunits ,Phenotype ,030220 oncology & carcinogenesis ,biology.protein ,Origin recognition complex ,HeLa Cells - Abstract
Replication Protein A (RPA) is a single-stranded DNA-binding protein essential for DNA replication, repair, recombination and cell-cycle regulation. A human homolog of the RPA2 subunit, called RPA4, was previously identified and shown to be expressed in colon mucosal and placental cells; however, the function of RPA4 was not determined. To examine the function of RPA4 in human cells, we carried out knockdown and replacement studies to determine whether RPA4 can substitute for RPA2 in the cell. Unlike RPA2, exogenous RPA4 expression did not support chromosomal DNA replication and lead to cell-cycle arrest in G2/M. In addition, RPA4 localized to sites of DNA repair and reduced gamma-H2AX caused by RPA2 depletion. These studies suggest that RPA4 cannot support cell proliferation but can support processes that maintain the genomic integrity of the cell.
- Published
- 2009
16. Cellular Functions of Human RPA1
- Author
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Aaron C. Mason, Stuart J. Haring, Sara K. Binz, and Marc S. Wold
- Subjects
HMG-box ,DNA repair ,DNA replication ,Eukaryotic DNA replication ,Cell Biology ,Biology ,complex mixtures ,Biochemistry ,Molecular biology ,DNA polymerase delta ,Cell biology ,enzymes and coenzymes (carbohydrates) ,Control of chromosome duplication ,Origin recognition complex ,Molecular Biology ,Replication protein A - Abstract
In eukaryotes, the single strand DNA (ssDNA)-binding protein, replication protein A (RPA), is essential for DNA replication, repair, and recombination. RPA is composed of the following three subunits: RPA1, RPA2, and RPA3. The RPA1 subunit contains four structurally related domains and is responsible for high affinity ssDNA binding. This study uses a depletion/replacement strategy in human cells to reveal the contributions of each domain to RPA cellular functions. Mutations that substantially decrease ssDNA binding activity do not necessarily disrupt cellular RPA function. Conversely, mutations that only slightly affect ssDNA binding can dramatically affect cellular function. The N terminus of RPA1 is not necessary for DNA replication in the cell; however, this region is important for the cellular response to DNA damage. Highly conserved aromatic residues in the high affinity ssDNA-binding domains are essential for DNA repair and cell cycle progression. Our findings suggest that as long as a threshold of RPA-ssDNA binding activity is met, DNA replication can occur and that an RPA activity separate from ssDNA binding is essential for function in DNA repair.
- Published
- 2008
17. Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
- Author
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David J. Marion, Marc S. Wold, Timothy J. Parnell, Emily J. Kuhn-Parnell, Pamela K. Geyer, Brian L. Gilmore, and Cecilia Helou
- Subjects
Chromatin Immunoprecipitation ,Molecular Sequence Data ,Repressor ,Investigations ,Eye ,DNA-binding protein ,Bacterial Proteins ,Silencer Elements, Transcriptional ,Genetics ,Animals ,Drosophila Proteins ,Transgenes ,Enhancer ,Alleles ,Sequence Deletion ,Zinc finger ,Binding Sites ,Base Sequence ,biology ,Pigmentation ,Serine Endopeptidases ,Zinc Fingers ,DNA ,biology.organism_classification ,DNA-Binding Proteins ,Repressor Proteins ,Drosophila melanogaster ,Enhancer Elements, Genetic ,Insulator Elements ,Chromatin Loop ,Chromatin immunoprecipitation ,Drosophila Protein - Abstract
Insulators define interactions between transcriptional control elements in eukaryotic genomes. The gypsy insulator found in the gypsy retrovirus binds the zinc-finger Suppressor of Hairy-wing [Su(Hw)] protein that associates with hundreds of non-gypsy regions throughout the Drosophila genome. Models of insulator function predict that the gypsy insulator forms chromatin loop domains through interactions with endogenous Su(Hw) insulators (SIs) to limit the action of transcriptional control elements. Here we study SI 62D and show that interactions occur between two SI 62D elements, but not between SI 62D and the gypsy insulator, limiting the scope of genomic gypsy insulator interactions. Enhancer blocking by SI 62D requires fewer Su(Hw)-binding sites than needed for gypsy insulator function, with these target regions having distinct zinc-finger requirements for in vivo Su(Hw) association. These observations led to an investigation of the role of the Su(Hw) zinc-finger domain in insulator function. Using a combination of in vitro and in vivo studies, we find that this domain makes sequence-dependent and -independent contributions to in vivo chromosome association, but is not essential for enhancer or silencer blocking. These studies extend our understanding of the properties of Su(Hw) and the endogenous genomic regions to which this protein localizes.
- Published
- 2008
18. Structure of the Full-length Human RPA14/32 Complex Gives Insights into the Mechanism of DNA Binding and Complex Formation
- Author
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Marc S. Wold, Edward H. Snell, Jeff E. Habel, Gloria E. O. Borgstahl, Venkataramen Kabaleeswaran, and Xiaoyi Deng
- Subjects
Models, Molecular ,Helix bundle ,Binding Sites ,Binding protein ,Molecular Sequence Data ,DNA replication ,DNA, Single-Stranded ,Plasma protein binding ,Biology ,Crystallography, X-Ray ,Dioxanes ,Protein Subunits ,Crystallography ,Protein structure ,Structural Biology ,Replication Protein A ,Humans ,Protein quaternary structure ,Amino Acid Sequence ,Binding site ,Protein Structure, Quaternary ,Molecular Biology ,Replication protein A ,Protein Binding - Abstract
Replication protein A (RPA) is the ubiquitous, eukaryotic single-stranded DNA (ssDNA) binding protein and is essential for DNA replication, recombination, and repair. Here, crystal structures of the soluble RPA heterodimer, composed of the RPA14 and RPA32 subunits, have been determined for the full-length protein in multiple crystal forms. In all crystals, the electron density for the N-terminal (residues 1-42) and C-terminal (residues 175-270) regions of RPA32 is weak and of poor quality indicating that these regions are disordered and/or assume multiple positions in the crystals. Hence, the RPA32 N terminus, that is hyperphosphorylated in a cell-cycle-dependent manner and in response to DNA damaging agents, appears to be inherently disordered in the unphosphorylated state. The C-terminal, winged helix-loop-helix, protein-protein interaction domain adopts several conformations perhaps to facilitate its interaction with various proteins. Although the ordered regions of RPA14/32 resemble the previously solved protease-resistant core crystal structure, the quaternary structures between the heterodimers are quite different. Thus, the four-helix bundle quaternary assembly noted in the original core structure is unlikely to be related to the quaternary structure of the intact heterotrimer. An organic ligand binding site between subunits RPA14 and RPA32 was identified to bind dioxane. Comparison of the ssDNA binding surfaces of RPA70 with RPA14/32 showed that the lower affinity of RPA14/32 can be attributed to a shallower binding crevice with reduced positive electrostatic charge.
- Published
- 2007
19. Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres
- Author
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Marc S. Wold, Per Eystein Lønning, Stig Ove Bøe, Rolf Bjerkvig, Åsne Jul-Larsen, Amra Grudic, and Stuart J. Haring
- Subjects
Telomerase ,Cell cycle checkpoint ,Cell Cycle ,DNA, Single-Stranded ,Telomere ,Cell cycle ,Biology ,Molecular biology ,chemistry.chemical_compound ,chemistry ,Medisinske Fag: 700 [VDP] ,RNA interference ,Cell Line, Tumor ,Neoplasms ,Replication Protein A ,Genetics ,Humans ,RNA Interference ,Molecular Biology ,Replication protein A ,Metaphase ,DNA ,Cell Line, Transformed - Abstract
The activation of a telomere maintenance mechanism is required for cancer development in humans. While most tumors achieve this by expressing the enzyme telomerase, a fraction (5–15%) employs a recombination-based mechanism termed alternative lengthening of telomeres (ALT). Here we show that loss of the single-stranded DNA-binding protein replication protein A (RPA) in human ALT cells, but not in telomerase-positive cells, causes increased exposure of single-stranded G-rich telomeric DNA, cell cycle arrest in G2/M phase, accumulation of single-stranded telomeric DNA within ALT-associated PML bodies (APBs), and formation of telomeric aggregates at the ends of metaphase chromosomes. This study demonstrates differences between ALT cells and telomerase-positive cells in the requirement for RPA in telomere processing and implicates the ALT mechanism in tumor cells as a possible therapeutic target. publishedVersion
- Published
- 2007
20. Replication Protein A Directs Loading of the DNA Damage Checkpoint Clamp to 5′-DNA Junctions
- Author
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Sara K. Binz, Jerzy Majka, Marc S. Wold, and Peter M. J. Burgers
- Subjects
Saccharomyces cerevisiae Proteins ,DNA polymerase ,DNA polymerase II ,Cell Cycle Proteins ,Eukaryotic DNA replication ,Biochemistry ,DNA polymerase delta ,Substrate Specificity ,Proliferating Cell Nuclear Antigen ,Replication Protein A ,Replication Protein C ,Molecular Biology ,Replication protein A ,Adenosine Triphosphatases ,DNA clamp ,biology ,DNA Helicases ,Intracellular Signaling Peptides and Proteins ,DNA replication ,Nuclear Proteins ,Cell Biology ,Endonucleases ,Molecular biology ,Cell biology ,Proliferating cell nuclear antigen ,DNA-Binding Proteins ,DNA Repair Enzymes ,biology.protein ,DNA Damage - Abstract
The heterotrimeric checkpoint clamp comprises the Saccharomyces cerevisiae Rad17, Mec3, and Ddc1 subunits (Rad17/3/1, the 9-1-1 complex in humans). This DNA damage response factor is loaded onto DNA by the Rad24-RFC (replication factor C-like complex with Rad24) clamp loader and ATP. Although Rad24-RFC alone does not bind to naked partial double-stranded DNA, coating of the single strand with single-stranded DNA-binding protein RPA (replication protein A) causes binding of Rad24-RFC via interactions with RPA. However, RPA-mediated binding is abrogated when the DNA is coated with RPA containing a rpa1-K45E (rfa1-t11) mutation. These properties allowed us to determine the role of RPA in clamp-loading specificity. The Rad17/3/1 clamp is loaded with comparable efficiency onto naked primer/template DNA with either a 3'-junction or a 5'-junction. Remarkably, when the DNA was coated with RPA, loading of Rad17/3/1 at 3'-junctions was completely inhibited, thereby providing specificity to loading at 5'-junctions. However, Rad17/3/1 loaded at 5'-junctions can slide across double-stranded DNA to nearby 3'-junctions and thereby affect the activity of proteins that act at 3'-termini. These studies show a unique specificity of the checkpoint loader for 5'-junctions of RPA-coated DNA. The implications of this specificity for checkpoint function are discussed.
- Published
- 2006
21. Identification of Genomic Sites That Bind the Drosophila Suppressor of Hairy-wing Insulator Protein
- Author
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Timothy J. Parnell, Marc S. Wold, Pamela K. Geyer, Cecilia Helou, Brian L. Gilmore, and Emily J. Kuhn
- Subjects
Chromatin Immunoprecipitation ,Transcription, Genetic ,Genome, Insect ,Biology ,DNA-binding protein ,Genome ,Transcriptional regulation ,Animals ,Drosophila Proteins ,Molecular Biology ,Gene ,Genetics ,Regulation of gene expression ,Binding Sites ,Articles ,Cell Biology ,biology.organism_classification ,DNA-Binding Proteins ,Repressor Proteins ,Drosophila melanogaster ,Enhancer Elements, Genetic ,Insulator Elements ,Chromatin immunoprecipitation ,Drosophila Protein ,Protein Binding - Abstract
Eukaryotic genomes are divided into independent transcriptional domains by DNA elements known as insulators. The gypsy insulator, a 350-bp element isolated from the Drosophila gypsy retrovirus, contains twelve degenerate binding sites for the Suppressor of Hairy-wing [Su(Hw)] protein. Su(Hw) associates with over 500 non-gypsy genomic sites, the functions of which are largely unknown. Using a bioinformatics approach, we identified 37 putative Su(Hw) insulators (pSIs) that represent regions containing clustered matches to the gypsy insulator Su(Hw) consensus binding sequence. The majority of these pSIs contain fewer than four Su(Hw) binding sites, with only seven showing in vivo Su(Hw) association, as demonstrated by chromatin immunoprecipitation. To understand the properties of the pSIs, these elements were tested for enhancer-blocking capabilities using a transgene assay system. In a complementary set of experiments, effects of the pSIs on transcriptional regulation of genes at the natural genomic location were determined. Our data suggest that pSIs have complex genomic functions and, in some cases, establish insulators. These studies provide the first direct evidence that the Su(Hw) protein contributes to the regulation of gene expression in the Drosophila genome through the establishment of endogenous insulators.
- Published
- 2006
22. Replication Protein A Interactions with DNA: Differential Binding of the Core Domains and Analysis of the DNA Interaction Surface
- Author
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Iwona M Wyka, Kajari Dhar, Sara K. Binz, and Marc S. Wold
- Subjects
Surface Properties ,Protein subunit ,DNA Mutational Analysis ,Genetic Vectors ,DNA, Single-Stranded ,Biology ,complex mixtures ,Biochemistry ,DNA-binding protein ,chemistry.chemical_compound ,Protein structure ,Replication Protein A ,Humans ,Point Mutation ,Replication protein A ,Conserved Sequence ,Point mutation ,Binding protein ,DNA-binding domain ,Molecular biology ,Protein Structure, Tertiary ,DNA-Binding Proteins ,Protein Subunits ,enzymes and coenzymes (carbohydrates) ,Models, Chemical ,chemistry ,Mutagenesis, Site-Directed ,Biophysics ,DNA ,Protein Binding - Abstract
Human replication protein A (RPA) is a heterotrimeric (70, 32, and 14 kDa subunits), eukaryotic single-stranded DNA (ssDNA) binding protein required for DNA recombination, repair, and replication. The three subunits of human RPA are composed of six conserved DNA binding domains (DBDs). Deletion and mutational studies have identified a high-affinity DNA binding core in the central region of the 70 kDa subunit, composed of DBDs A and B. To define the roles of each DBD in DNA binding, monomeric and tandem DBD A and B domain chimeras were created and characterized. Individually, DBDs A and B have a very low intrinsic affinity for ssDNA. In contrast, tandem DBDs (AA, AB, BA, and BB) bind ssDNA with moderate to high affinity. The AA chimera had a much higher affinity for ssDNA than did the other tandem DBDs, demonstrating that DBD A has a higher intrinsic affinity for ssDNA than DBD B. The RPA-DNA interface is similar in both DBD A and DBD B. Mutational analysis was carried out to probe the relative contributions of the two domains to DNA binding. Mutation of polar residues in either core DBD resulted in a significant decrease in the affinity of the RPA complex for ssDNA. RPA complexes with pairs of mutated polar residues had lower affinities than those with single mutations. The decrease in affinity observed when polar mutations were combined suggests that multiple polar interactions contribute to the affinity of the RPA core for DNA. These results indicate that RPA-ssDNA interactions are the result of binding of multiple nonequivalent domains. Our data are consistent with a sequential binding model for RPA, in which DBD A is responsible for positioning and initial binding of the RPA complex while DBD A together with DBD B direct stable, high-affinity binding to ssDNA.
- Published
- 2003
23. The Phosphorylation Domain of the 32-kDa Subunit of Replication Protein A (RPA) Modulates RPA-DNA Interactions
- Author
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David F. Lowry, Ye Lao, Sara K. Binz, and Marc S. Wold
- Subjects
DNA damage ,Protein subunit ,Mutagenesis ,DNA replication ,Cell Biology ,Biology ,complex mixtures ,Biochemistry ,Molecular biology ,enzymes and coenzymes (carbohydrates) ,chemistry.chemical_compound ,Protein structure ,chemistry ,Biophysics ,Phosphorylation ,Molecular Biology ,Replication protein A ,DNA - Abstract
Replication protein A (RPA) is a heterotrimeric (subunits of 70, 32, and 14 kDa) single-stranded DNA-binding protein that is required for DNA replication, recombination, and repair. The 40-residue N-terminal domain of the 32-kDa subunit of RPA (RPA32) becomes phosphorylated during S-phase and after DNA damage. Recently it has been shown that phosphorylation or the addition of negative charges to this N-terminal phosphorylation domain modulates RPA-protein interactions and increases cell sensitivity to DNA damage. We found that addition of multiple negative charges to the N-terminal phosphorylation domain also caused a significant decrease in the ability of a mutant form of RPA to destabilize double-stranded (ds) DNA. Kinetic studies suggested that the addition of negative charges to the N-terminal phosphorylation domain caused defects in both complex formation (nucleation) and subsequent destabilization of dsDNA by RPA. We conclude that the N-terminal phosphorylation domain modulates RPA interactions with dsDNA. Similar changes in DNA interactions were observed with a mutant form of RPA in which the N-terminal domain of the 70-kDa subunit was deleted. This suggested a functional link between the N-terminal domains of the 70- and 32-kDa subunits of RPA. NMR experiments provided evidence for a direct interaction between the N-terminal domain of the 70-kDa subunit and the negatively charged N-terminal phosphorylation domain of RPA32. These findings suggest that phosphorylation causes a conformational change in the RPA complex that regulates RPA function.
- Published
- 2003
24. Chemical shift changes provide evidence for overlapping single-stranded DNA- and XPA-binding sites on the 70 kDa subunit of human replication protein A
- Author
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Marc S. Wold, Michael A. Kennedy, Cheryl H. Arrowsmith, Garry W. Buchko, Gary W. Daughdrill, Maria Victoria Botuyan, and David F. Lowry
- Subjects
Models, Molecular ,endocrine system ,Protein subunit ,DNA, Single-Stranded ,Plasma protein binding ,Biology ,chemistry.chemical_compound ,Replication Protein A ,Genetics ,Humans ,Binding site ,Nuclear Magnetic Resonance, Biomolecular ,Replication protein A ,Ternary complex ,Binding Sites ,Binding protein ,Articles ,Peptide Fragments ,Xeroderma Pigmentosum Group A Protein ,DNA-Binding Proteins ,Protein Subunits ,enzymes and coenzymes (carbohydrates) ,Biochemistry ,chemistry ,Biophysics ,DNA ,Protein Binding ,Nucleotide excision repair - Abstract
Replication protein A (RPA) is a heterotrimeric single-stranded DNA- (ssDNA) binding protein that can form a complex with the xeroderma pigmentosum group A protein (XPA). This complex can preferentially recognize UV-damaged DNA over undamaged DNA and has been implicated in the stabilization of open complex formation during nucleotide excision repair. In this report, nuclear magnetic resonance (NMR) spectroscopy was used to investigate the interaction between a fragment of the 70 kDa subunit of human RPA, residues 1-326 (hRPA70(1-326)), and a fragment of the human XPA protein, residues 98-219 (XPA-MBD). Intensity changes were observed for amide resonances in the (1)H-(15)N correlation spectrum of uniformly (15)N-labeled hRPA70(1-326) after the addition of unlabeled XPA-MBD. The intensity changes observed were restricted to an ssDNA-binding domain that is between residues 183 and 296 of the hRPA70(1-326) fragment. The hRPA70(1-326) residues with the largest resonance intensity reductions were mapped onto the structure of the ssDNA-binding domain to identify the binding surface with XPA-MBD. The XPA-MBD-binding surface showed significant overlap with an ssDNA-binding surface that was previously identified using NMR spectroscopy and X-ray crystallography. Overlapping XPA-MBD- and ssDNA-binding sites on hRPA70(1-326) suggests that a competitive binding mechanism mediates the formation of the RPA-XPA complex. To determine whether a ternary complex could form between hRPA70(1-326), XPA-MBD and ssDNA, a (1)H-(15)N correlation spectrum was acquired for uniformly (15)N-labeled hRPA70(1-326) after the simultaneous addition of unlabeled XPA-MBD and ssDNA. In this experiment, the same chemical shift perturbations were observed for hRPA70(1-326) in the presence of XPA-MBD and ssDNA as was previously observed in the presence of ssDNA alone. The ability of ssDNA to compete with XPA-MBD for an overlapping binding site on hRPA70(1-326) suggests that any complex formation between RPA and XPA that involves the interaction between XPA-MBD and hRPA70(1-326) may be modulated by ssDNA.
- Published
- 2003
25. Analysis of the Human Replication Protein A:Rad52 Complex: Evidence for Crosstalk Between RPA32, RPA70, Rad52 and DNA
- Author
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James K Wahl, Gloria E. O. Borgstahl, Marc S. Wold, Kajari Dhar, and Doba Jackson
- Subjects
DNA Replication ,DNA Repair ,Light ,HMG-box ,Macromolecular Substances ,DNA repair ,Molecular Sequence Data ,genetic processes ,RAD52 ,RAD51 ,DNA, Single-Stranded ,Electrophoretic Mobility Shift Assay ,Enzyme-Linked Immunosorbent Assay ,Simian virus 40 ,Biology ,Binding, Competitive ,complex mixtures ,Structural Biology ,Replication Protein A ,Humans ,Scattering, Radiation ,Amino Acid Sequence ,Binding site ,Molecular Biology ,Replication protein A ,Genetics ,Osmolar Concentration ,fungi ,DNA replication ,Surface Plasmon Resonance ,Precipitin Tests ,Protein Structure, Tertiary ,Rad52 DNA Repair and Recombination Protein ,Cell biology ,DNA-Binding Proteins ,enzymes and coenzymes (carbohydrates) ,Mutation ,Rad51 Recombinase ,Homologous recombination ,DNA Damage ,Protein Binding - Abstract
The eukaryotic single-stranded DNA-binding protein, replication protein A (RPA), is essential for DNA replication, and plays important roles in DNA repair and DNA recombination. Rad52 and RPA, along with other members of the Rad52 epistasis group of genes, repair double-stranded DNA breaks (DSBs). Two repair pathways involve RPA and Rad52, homologous recombination and single-strand annealing. Two binding sites for Rad52 have been identified on RPA. They include the previously identified C-terminal domain (CTD) of RPA32 (residues 224-271) and the newly identified domain containing residues 169-326 of RPA70. A region on Rad52, which includes residues 218-303, binds RPA70 as well as RPA32. The N-terminal region of RPA32 does not appear to play a role in the formation of the RPA:Rad52 complex. It appears that the RPA32CTD can substitute for RPA70 in binding Rad52. Sequence homology between RPA32 and RPA70 was used to identify a putative Rad52-binding site on RPA70 that is located near DNA-binding domains A and B. Rad52 binding to RPA increases ssDNA affinity significantly. Mutations in DBD-D on RPA32 show that this domain is primarily responsible for the ssDNA binding enhancement. RPA binding to Rad52 inhibits the higher-order self-association of Rad52 rings. Implications for these results for the "hand-off" mechanism between protein-protein partners, including Rad51, in homologous recombination and single-strand annealing are discussed.
- Published
- 2002
26. Replication protein A: single-stranded DNA's first responder: dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair
- Author
-
Ran Chen and Marc S. Wold
- Subjects
DNA Replication ,Models, Molecular ,HMG-box ,DNA Repair ,DNA repair ,DNA, Single-Stranded ,Biology ,medicine.disease_cause ,complex mixtures ,Binding, Competitive ,General Biochemistry, Genetics and Molecular Biology ,Article ,chemistry.chemical_compound ,Gene Expression Regulation, Fungal ,Replication Protein A ,medicine ,Ustilago ,DNA, Fungal ,Replication protein A ,Single-strand DNA-binding protein ,Recombination, Genetic ,Mutation ,DNA synthesis ,DNA replication ,Molecular biology ,Cell biology ,Protein Structure, Tertiary ,enzymes and coenzymes (carbohydrates) ,chemistry ,DNA ,Protein Binding ,Signal Transduction - Abstract
Replication protein A (RPA), the major single-stranded DNA-binding protein in eukaryotic cells, is required for processing of single-stranded DNA (ssDNA) intermediates found in replication, repair, and recombination. Recent studies have shown that RPA binding to ssDNA is highly dynamic and that more than high-affinity binding is needed for function. Analysis of DNA binding mutants identified forms of RPA with reduced affinity for ssDNA that are fully active, and other mutants with higher affinity that are inactive. Single molecule studies showed that while RPA binds ssDNA with high affinity, the RPA complex can rapidly diffuse along ssDNA and be displaced by other proteins that act on ssDNA. Finally, dynamic DNA binding allows RPA to prevent error-prone repair of double-stranded breaks and promote error-free repair. Together, these findings suggest a new paradigm where RPA acts as a first responder at sites with ssDNA, thereby actively coordinating DNA repair and DNA synthesis.
- Published
- 2014
27. Diffusion of human Replication Protein A along single stranded DNA
- Author
-
Binh Nguyen, Roberto Galletto, Joshua E. Sokoloski, Elliot L. Elson, Timothy M. Lohman, and Marc S. Wold
- Subjects
DNA Replication ,DNA Repair ,DNA repair ,viruses ,genetic processes ,DNA, Single-Stranded ,Plasma protein binding ,Nucleic Acid Denaturation ,environment and public health ,Article ,Nucleic acid secondary structure ,chemistry.chemical_compound ,Structural Biology ,Replication Protein A ,Humans ,Molecular Biology ,Replication protein A ,Fluorescent Dyes ,Gene Rearrangement ,Recombination, Genetic ,DNA replication ,Gene rearrangement ,Carbocyanines ,Single-molecule experiment ,Molecular biology ,enzymes and coenzymes (carbohydrates) ,chemistry ,Biophysics ,health occupations ,DNA ,Protein Binding - Abstract
Replication protein A (RPA) is a eukaryotic single-stranded DNA (ssDNA) binding protein that plays critical roles in most aspects of genome maintenance, including replication, recombination and repair. RPA binds ssDNA with high affinity, destabilizes DNA secondary structure and facilitates binding of other proteins to ssDNA. However, RPA must be removed from or redistributed along ssDNA during these processes. To probe the dynamics of RPA-DNA interactions, we combined ensemble and single-molecule fluorescence approaches to examine human RPA (hRPA) diffusion along ssDNA and find that an hRPA heterotrimer can diffuse rapidly along ssDNA. Diffusion of hRPA is functional in that it provides the mechanism by which hRPA can transiently disrupt DNA hairpins by diffusing in from ssDNA regions adjacent to the DNA hairpin. hRPA diffusion was also monitored by the fluctuations in fluorescence intensity of a Cy3 fluorophore attached to the end of ssDNA. Using a novel method to calibrate the Cy3 fluorescence intensity as a function of hRPA position on the ssDNA, we estimate a one-dimensional diffusion coefficient of hRPA on ssDNA of D1~5000nt(2) s(-1) at 37°C. Diffusion of hRPA while bound to ssDNA enables it to be readily repositioned to allow other proteins access to ssDNA.
- Published
- 2014
28. Replication protein A modulates its interface with the primed DNA template during RNA-DNA primer elongation in replicating SV40 chromosomes
- Author
-
Gilad Mass, Gabriel Kaufmann, Olga I. Lavrik, Marc S. Wold, and Tamar Nethanel
- Subjects
DNA Replication ,Protein subunit ,Oligonucleotides ,Genome, Viral ,Photoaffinity Labels ,Simian virus 40 ,Biology ,Binding, Competitive ,Article ,chemistry.chemical_compound ,Replication Protein A ,Genetics ,Animals ,Humans ,Replication protein A ,DNA Primers ,Okazaki fragments ,Oligonucleotide ,DNA replication ,RNA ,Templates, Genetic ,Molecular biology ,Cell biology ,DNA-Binding Proteins ,Protein Subunits ,Cross-Linking Reagents ,chemistry ,DNA, Viral ,Primer (molecular biology) ,DNA ,Protein Binding - Abstract
The eukaryal single-stranded DNA binding protein replication protein A (RPA) binds short oligonucleotides with high affinity but exhibits low cooperativity in binding longer templates, opposite to prokaryal counterparts. This discrepancy could reflect the smaller size of the replicative template portion availed to RPA. According to current models, this portion accommodates an RNA–DNA primer (RDP) of
- Published
- 2001
29. Purification and Characterization of ATM from Human Placenta
- Author
-
Pauline Douglas, Ruiqiong Ye, Yoichi Taya, Doug W. Chan, Martin F. Lavin, Wesley D. Block, Marc S. Wold, Jennifer L. Pelley, Aaron A. Goodarzi, Seong-Cheol Son, Kum Kum Khanna, and Susan P. Lees-Miller
- Subjects
Serine/threonine-specific protein kinase ,Cell Biology ,Serine threonine protein kinase ,Biology ,medicine.disease ,environment and public health ,Biochemistry ,MAP2K7 ,enzymes and coenzymes (carbohydrates) ,Ataxia-telangiectasia ,medicine ,Protein phosphorylation ,Protein kinase A ,Molecular Biology ,Ataxia telangiectasia and Rad3 related ,DNA-PKcs - Abstract
ATM is mutated in the human genetic disorder ataxia telangiectasia, which is characterized by ataxia, immune defects, and cancer predisposition. Cells that lack ATM exhibit delayed up-regulation of p53 in response to ionizing radiation. Serine 15 of p53 is phosphorylated in vivo in response to ionizing radiation, and antibodies to ATM immunoprecipitate a protein kinase activity that, in the presence of manganese, phosphorylates p53 at serine 15. Immunoprecipitates of ATM also phosphorylate PHAS-I in a manganese-dependent manner. Here we have purified ATM from human cells using nine chromatographic steps. Highly purified ATM phosphorylated PHAS-I, the 32-kDa subunit of RPA, serine 15 of p53, and Chk2 in vitro. The majority of the ATM phosphorylation sites in Chk2 were located in the amino-terminal 57 amino acids. In each case, phosphorylation was strictly dependent on manganese. ATM protein kinase activity was inhibited by wortmannin with an IC(50) of approximately 100 nM. Phosphorylation of RPA, but not p53, Chk2, or PHAS-I, was stimulated by DNA. The related protein, DNA-dependent protein kinase catalytic subunit, also phosphorylated PHAS-I, RPA, and Chk2 in the presence of manganese, suggesting that the requirement for manganese is a characteristic of this class of enzyme.
- Published
- 2000
30. Analysis of the capacity of extracts from normal human young and senescent fibroblasts to support DNA synthesis in vitro
- Author
-
W.R. Pendergrass, Matthew D. Gray, P. Luo, Marc S. Wold, and Thomas H. Norwood
- Subjects
biology ,DNA synthesis ,Chemistry ,DNA polymerase ,DNA polymerase II ,DNA replication ,Eukaryotic DNA replication ,Cell Biology ,Biochemistry ,Molecular biology ,law.invention ,Proliferating cell nuclear antigen ,law ,biology.protein ,Recombinant DNA ,Molecular Biology ,Replication protein A - Abstract
Cytoplasmic extracts from early-passage (young), late-passage (senescent) normal human fibroblast (HF) cultures and immortalized human cell lines (HeLa, HT-1080, and MANCA) were analyzed for their ability to support semiconservative DNA synthesis in an in vitro SV40-ori DNA replication system. Unsupplemented extracts from the three permanent cell lines were demonstrated to be active in this system; whereas young HF extracts were observed to be minimally active, and no activity could be detected in the senescent HF extracts. The activity of these extracts was compared after supplementation with three recombinant human replication factors: (1) the catalytic subunit of DNA polymerase alpha (DNA pol-alpha-cat), (2) the three subunits of replication protein A (RPA), and (3) DNA topoisomerase I (Topo I). The addition of all three recombinant proteins is required for optimum activity in the young and senescent HF extracts; the order of the level of activity is: transformed > young HF > senescent HF. Young HF extracts supplemented with RPA alone are able to support significant replicative activity but not senescent extracts which require both RPA and DNA pol-alpha-cat for any detectable activity. The necessary requirement for these factors is confirmed by the failure of unsupplemented young and senescent extracts to activate MANCA extracts that have been immunodepleted of DNA pol-alpha-cat or RPA. Immunocytochemical studies revealed that RPA, DNA pol-alpha, PCNA, and topo I levels are higher in the immortal cell types used in these studies. In the HF cells, levels of DNA pol-alpha-cat and PCNA are higher (per mg protein) in the low-passage than in the senescent cells. By contrast, RPA levels, as determined by immunocytochemical or Western blot studies, were observed to be similar in both young and senescent cell nuclei. Taken together, these results indicate that the low to undetectable activity of young HF extracts in this system is due mainly to reduced intracellular levels of RPA, while the senescent HF extracts are relatively deficient in DNA polymerase alpha and probably some other essential replication factors, as well as RPA. Moreover, the retention of RPA in the senescent HF nuclei contributes to the low level of this factor in the cytoplasmic extracts from these cells.
- Published
- 1999
31. Replication Protein A Interactions with DNA. 1. Functions of the DNA-Binding and Zinc-Finger Domains of the 70-kDa Subunit
- Author
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Chang Geun Lee, André P. Walther, Marc S. Wold, Ye Lao, and Xavier V. Gomes
- Subjects
HMG-box ,DNA, Single-Stranded ,Simian virus 40 ,complex mixtures ,Biochemistry ,Replication factor C ,SeqA protein domain ,Replication Protein A ,Humans ,Antigens, Viral, Tumor ,Replication protein A ,LIM domain ,Xeroderma Pigmentosum ,Chemistry ,Ter protein ,Zinc Fingers ,DNA ,DNA-binding domain ,Peptide Fragments ,Xeroderma Pigmentosum Group A Protein ,Cell biology ,DNA-Binding Proteins ,enzymes and coenzymes (carbohydrates) ,Mutagenesis, Site-Directed ,Origin recognition complex ,Protein Binding - Abstract
Human replication protein A (RPA) is a multiple subunit single-stranded DNA-binding protein that is required for multiple processes in cellular DNA metabolism. This complex, composed of subunits of 70, 32, and 14 kDa, binds to single-stranded DNA (ssDNA) with high affinity and participates in multiple protein-protein interactions. The 70-kDa subunit of RPA is known to be composed of multiple domains: an N-terminal domain that participates in protein interactions, a central DNA-binding domain (composed of two copies of a ssDNA-binding motif), a putative (C-X2-C-X13-C-X2-C) zinc finger, and a C-terminal intersubunit interaction domain. A series of mutant forms of RPA were used to elucidate the roles of these domains in RPA function. The central DNA-binding domain was necessary and sufficient for interactions with ssDNA; however, adjacent sequences, including the zinc-finger domain and part of the N-terminal domain, were needed for optimal ssDNA-binding activity. The role of aromatic residues in RPA-DNA interactions was examined. Mutation of any one of the four aromatic residues shown to interact with ssDNA had minimal effects on RPA activity, indicating that individually these residues are not critical for RPA activity. Mutation of the zinc-finger domain altered the structure of the RPA complex, reduced ssDNA-binding activity, and eliminated activity in DNA replication.
- Published
- 1999
32. [Untitled]
- Author
-
Zita A. Sibenaller, Marc S. Wold, Jamboor K. Vishwanatha, Yungping Chiang, and Angie Rizzino
- Subjects
DNA synthesis ,Cell growth ,Clinical Biochemistry ,DNA replication ,Tyrosine phosphorylation ,Cell Biology ,General Medicine ,Transfection ,Biology ,Molecular biology ,Cell biology ,chemistry.chemical_compound ,chemistry ,Annexin ,Molecular Biology ,Annexin A2 ,Annexin A1 - Abstract
The protein-tyrosine kinase substrate annexin II is a growth regulated gene whose expression is increased in several human cancers. While the precise function of this protein is not understood, annexin II is proposed to be involved in multiple physiological activities, including DNA synthesis and cell proliferation. Targeted disruption of the annexin II gene affects calcium signaling, tyrosine phosphorylation and apoptosis, indicating the important physiological role of this protein. We used a transient co-transfection assay to regulate annexin II expression in human HeLa, 293 and 293T cells, and measured the effects of annexin II down regulation on DNA synthesis and proliferation. Transfection of cells with an antisense annexin II vector results in inhibition of cell division and proliferation, with concomitant reduction in annexin II message and protein levels. Cellular DNA synthesis is significantly reduced in antisense transfected cells. Replication extracts made from antisense transfected cells have significantly reduced efficiency to support SV40 in vitro DNA replication, while the extracts made from sense transfected cells are fully capable of replication. Our results indicate an important role of annexin II in cellular DNA synthesis and cell proliferation.
- Published
- 1999
33. [Untitled]
- Author
-
Marc S. Wold, Andrew S. Lipton, Nancy G. Isern, Xavier V. Gomes, Doris M. Jacobs, Gary W. Daughdrill, and David F. Lowry
- Subjects
biology ,HMG-box ,Chemistry ,DNA polymerase ,DNA repair ,DNA replication ,Biochemistry ,Molecular biology ,Cell biology ,chemistry.chemical_compound ,SeqA protein domain ,biology.protein ,B3 domain ,Replication protein A ,Spectroscopy ,DNA - Abstract
Human Replication Protein A (hsRPA) is required for multiple cellular processes in DNA metabolism including DNA repair, replication and recombination. It binds single-stranded DNA with high affinity and interacts specifically with multiple proteins. hsRPA forms a heterotrimeric complex composed of 70-, 32- and 14-kDa subunits (henceforth RPA70, RPA32, and RPA14). The N-terminal 168 residues of RPA70 form a structurally distinct domain that stimulates DNA polymerase α activity, interacts with several transcriptional activators including tumor suppressor p53, and during the cell cycle it signals escape from the DNA damage induced G2/M checkpoint. We have solved the global fold of the fragment corresponding to this domain (RPA70Δ169) and we find residues 8–108 of the N-terminal domain are structured. The remaining C-terminal residues are unstructured and may form a flexible linker to the DNA-binding domain of RPA70. The globular region forms a five-stranded anti-parallel β-barrel. The ends of the barrel are capped by short helices. Two loops on one side of the barrel form a large basic cleft which is a likely site for binding the acidic motifs of transcriptional activators. Many lethal or conditional lethal yeast point mutants map to this cleft, whereas no mutations with severe phenotype have been found in the linker region.
- Published
- 1999
34. Interaction of human Rad51 recombination protein with single-stranded DNA binding protein, RPA
- Author
-
Thomas Haaf, Marc S. Wold, Efim I. Golub, Ravindra C. Gupta, and Charles M. Radding
- Subjects
Immunoprecipitation ,Protein subunit ,RAD51 ,DNA, Single-Stranded ,Biology ,Peptide Mapping ,complex mixtures ,DNA-binding protein ,Mice ,chemistry.chemical_compound ,Replication Protein A ,Heterotrimeric G protein ,Genetics ,medicine ,Animals ,Humans ,Replication protein A ,Cells, Cultured ,Recombination, Genetic ,Fibroblasts ,Precipitin Tests ,Molecular biology ,Peptide Fragments ,Rats ,DNA-Binding Proteins ,Molecular Weight ,enzymes and coenzymes (carbohydrates) ,Cell nucleus ,medicine.anatomical_structure ,chemistry ,Gamma Rays ,Rad51 Recombinase ,DNA ,Research Article - Abstract
Replication protein A (RPA), a heterotrimeric single-stranded DNA binding protein, is required for recombination, and stimulates homologous pairing and DNA strand exchange promoted in vitro by human recombination protein HsRad51. Co-immunoprecipitation revealed that purified RPA interacts physically with HsRad51, as well as with HsDmc1, the homolog that is expressed specifically in meiosis. The interaction with HsRad51 was mediated by the 70 kDa subunit of RPA, and according to experiments with deletion mutants, this interaction required amino acid residues 169-326. In exponentially growing mammalian cells, 22% of nuclei showed foci of RPA protein and 1-2% showed foci of Rad51. After gamma-irradiation, the percentage of cells with RPA foci increased to approximately 50%, and those with Rad51 foci to 30%. All of the cells with foci of Rad51 had foci of RPA, and in those cells the two proteins co-localized in a high fraction of foci. The interactions of human RPA with Rad51, replication proteins and DNA are suited to the linking of recombination to replication.
- Published
- 1998
35. The 32- and 14-Kilodalton Subunits of Replication Protein A Are Responsible for Species-Specific Interactions with Single-Stranded DNA
- Author
-
Zita A. Sibenaller, Brenda R. Sorensen, and Marc S. Wold
- Subjects
enzymes and coenzymes (carbohydrates) ,Replication factor C ,Biochemistry ,Chemistry ,Protein subunit ,Ter protein ,DNA replication ,Origin recognition complex ,Cooperativity ,complex mixtures ,Replication protein A ,DNA polymerase delta - Abstract
Replication protein A (RPA) is a multisubunit single-stranded DNA-binding (ssDNA) protein that is required for cellular DNA metabolism. RPA homologues have been identified in all eukaryotes examined. All homologues are heterotrimeric complexes with subunits of approximately 70, approximately 32, and approximately 14 kDa. While RPA homologues are evolutionarily conserved, they are not functionally equivalent. To gain a better understanding of the functional differences between RPA homologues, we analyzed the DNA-binding parameters of RPA from human cells and the budding yeast Saccharomyces cerevisiae (hRPA and scRPA, respectively). Both yeast and human RPA bind ssDNA with high affinity and low cooperativity. However, scRPA has a larger occluded binding site (45 nucleotides versus 34 nucleotides) and a higher affinity for oligothymidine than hRPA. Mutant forms of hRPA and scRPA containing the high-affinity DNA-binding domain from the 70-kDa subunit had nearly identical DNA binding properties. In contrast, subcomplexes of the 32- and 14-kDa subunits from both yeast and human RPA had weak ssDNA binding activity. However, the binding constants for the yeast and human subcomplexes were 3 and greater than 6 orders of magnitude lower than those for the RPA heterotrimer, respectively. We conclude that differences in the activity of the 32- and 14-kDa subunits of RPA are responsible for variations in the ssDNA-binding properties of scRPA and hRPA. These data also indicate that hRPA and scRPA have different modes of binding to ssDNA, which may contribute to the functional disparities between the two proteins.
- Published
- 1998
36. Role of Protein−Protein Interactions in the Function of Replication Protein A (RPA): RPA Modulates the Activity of DNA Polymerase α by Multiple Mechanisms
- Author
-
C. J. Ingles, Marc S. Wold, Zhigang He, Lao Y, and Braun Ka
- Subjects
DNA Replication ,Antigens, Polyomavirus Transforming ,DNA polymerase II ,DNA, Single-Stranded ,complex mixtures ,Biochemistry ,DNA polymerase delta ,Fluorescence ,DNA replication factor CDT1 ,Replication factor C ,SeqA protein domain ,Replication Protein A ,Humans ,Replication protein A ,biology ,Chemistry ,DNA replication ,Herpes Simplex Virus Protein Vmw65 ,DNA Polymerase II ,Cell biology ,DNA-Binding Proteins ,Enzyme Activation ,enzymes and coenzymes (carbohydrates) ,Mutation ,biology.protein ,Origin recognition complex ,Electrophoresis, Polyacrylamide Gel ,Protein Binding - Abstract
Replication Protein A (RPA) from human cells is a stable complex of 70-, 32-, and 14-kDa subunits that is required for multiple processes in DNA metabolism. RPA binds with high affinity to single-stranded DNA and interacts with multiple proteins, including proteins required for the initiation of SV40 DNA replication, DNA polymerase alpha and SV40 large T antigen. We have used a series of mutant derivatives of RPA to map the regions of RPA required for specific protein-protein interactions and have examined the roles of these interactions in DNA replication. T antigen, DNA polymerase alpha and the activation domain of VP16 all have overlapping sites of interaction in the N-terminal half (residues 1-327) of the 70-kDa subunit of RPA. In addition, the interaction site for DNA polymerase alpha is composed of two functionally distinct regions, one (residues 1- approximately 170) which stimulates polymerase activity and a second (residues approximately 170-327) which increases polymerase processivity. In the latter, both the direct protein-protein interaction and ssDNA-binding activities of RPA were needed for RPA to modulate polymerase processivity. We also found that SV40 T antigen inhibited the ability of RPA to increase processivity of DNA polymerase alpha, suggesting that this activity of RPA may be important for elongation but not during the initiation of DNA replication. DNA polymerase alpha, but not T antigen also interacted with the 32- and/or 14-kDa subunits of RPA, but these interactions did not seem to effect polymerase activity.
- Published
- 1997
37. REPLICATION PROTEIN A: A Heterotrimeric, Single-Stranded DNA-Binding Protein Required for Eukaryotic DNA Metabolism
- Author
-
Marc S. Wold
- Subjects
DNA Replication ,DNA Repair ,biology ,Molecular Sequence Data ,DNA replication ,Eukaryotic DNA replication ,DNA ,complex mixtures ,Biochemistry ,Cell biology ,Single-stranded binding protein ,Replication protein A2 ,DNA-Binding Proteins ,DNA replication factor CDT1 ,enzymes and coenzymes (carbohydrates) ,Replication factor C ,Replication Protein A ,biology.protein ,Humans ,Origin recognition complex ,Protein Processing, Post-Translational ,Replication protein A ,DNA Damage ,Protein Binding - Abstract
Replication protein A [RPA; also known as replication factor A (RFA) and human single-stranded DNA-binding protein] is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism, including DNA replication, DNA repair, and recombination. RPA homologues have been identified in all eukaryotic organisms examined and are all abundant heterotrimeric proteins composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. In cells, RPA is phosphorylated by DNA-dependent protein kinase when RPA is bound to single-stranded DNA (during S phase and after DNA damage). Phosphorylation of RPA may play a role in coordinating DNA metabolism in the cell. RPA may also have a role in modulating gene expression.
- Published
- 1997
38. Replication Protein A Is a Component of a Complex That Binds the Human Metallothionein IIA Gene Transcription Start Site
- Author
-
Alan E. Tomkinson, William S. Lane, Edward Seto, Marc S. Wold, and Chih Min Tang
- Subjects
DNA Replication ,Transcription, Genetic ,Molecular Sequence Data ,Response element ,DNA, Recombinant ,DNA, Single-Stranded ,E-box ,RNA polymerase II ,Biochemistry ,Sp3 transcription factor ,Replication Protein A ,Humans ,Amino Acid Sequence ,Enhancer ,Molecular Biology ,Base Sequence ,biology ,General transcription factor ,Promoter ,Cell Biology ,Molecular biology ,DNA-Binding Proteins ,TAF2 ,biology.protein ,Metallothionein ,Protein Binding - Abstract
Previous studies revealed that sequences surrounding the initiation sites in many mammalian and viral gene promoters, called initiator (Inr) elements, may be essential for promoter strength and for determining the actual transcription start sites. DNA sequences in the vicinity of the human metallothionein IIA (hMTIIA) gene transcription start site share homology with some of the previously identified Inr elements. However, in the present study we have found by in vitro transcription assays that the hMTIIA promoter does not contain a typical Inr. Electrophoretic mobility shift assays identified several DNA-protein complexes at the hMTIIA gene transcription start site. A partially purified protein fraction containing replication protein A (RPA) binds to the hMTIIA gene transcription start site and represses transcription from the hMTIIA promoter in vitro. In addition, overexpression of the human 70-kDa RPA-1 protein represses transcription of a reporter gene controlled by the hMTIIA promoter in vivo. These findings suggest that hMTIIA transcription initiation is controlled by a mechanism different from most mammalian and viral promoters and that the previously identified RPA may also be involved in transcription regulation.
- Published
- 1996
39. Phosphorylation of human replication protein A by the DNA-dependent protein kinase is involved in the modulation of DNA replication
- Author
-
Anindya Dutta, Marc S. Wold, Timothy H. Carter, and Leigh A. Henricksen
- Subjects
DNA Replication ,Protein Conformation ,DNA, Single-Stranded ,Eukaryotic DNA replication ,DNA-Activated Protein Kinase ,Simian virus 40 ,Protein Serine-Threonine Kinases ,Biology ,DNA replication factor CDT1 ,Structure-Activity Relationship ,Replication factor C ,Control of chromosome duplication ,Replication Protein A ,Genetics ,Humans ,Phosphorylation ,Replication protein A ,Sequence Deletion ,Ter protein ,DNA replication ,Nuclear Proteins ,DNA-Binding Proteins ,enzymes and coenzymes (carbohydrates) ,Biochemistry ,DNA, Viral ,Mutation ,biology.protein ,Origin recognition complex ,Protein Binding ,Research Article - Abstract
The single-stranded DNA-binding protein, Replication Protein A (RPA), is a heterotrimeric complex with subunits of 70, 32 and 14 kDa involved in DNA metabolism. RPA may be a target for cellular regulation; the 32 kDa subunit (RPA32) is phosphorylated by several cellular kinases including the DNA-dependent protein kinase (DNA-PK). We have purified a mutant hRPA complex lacking amino acids 1-33 of RPA32 (rhRPA x 32delta1-33). This mutant bound ssDNA and supported DNA replication; however, rhRPA x 32delta1-33 was not phosphorylated under replication conditions or directly by DNA-PK. Proteolytic mapping revealed that all the sites phosphorylated by DNA-PK are contained on residues 1-33 of RPA32. When wild-type RPA was treated with DNA-PK and the mixture added to SV40 replication assays, DNA replication was supported. In contrast, when rhRPA x 32delta1-33 was treated with DNA-PK, DNA replication was strongly inhibited. Because untreated rhRPA x 32delta1-33 is fully functional, this suggests that the N-terminus of RPA is needed to overcome inhibitory effects of DNA-PK on other components of the DNA replication system. Thus, phosphorylation of RPA may modulate DNA replication indirectly, through interactions with other proteins whose activity is modulated by phosphorylation.
- Published
- 1996
40. Functional Domains of the 70-Kilodalton Subunit of Human Replication Protein A
- Author
-
Marc S. Wold and Xavier V. Gomes
- Subjects
DNA Replication ,Antigens, Polyomavirus Transforming ,Molecular Sequence Data ,DNA, Single-Stranded ,Replication Origin ,Eukaryotic DNA replication ,Simian virus 40 ,Biochemistry ,Replication factor C ,Control of chromosome duplication ,Minichromosome maintenance ,Replication Protein A ,Humans ,Phosphorylation ,Replication protein A ,Sequence Deletion ,Base Sequence ,Chemistry ,Ter protein ,DNA replication ,Cell biology ,DNA-Binding Proteins ,enzymes and coenzymes (carbohydrates) ,Mutagenesis ,DNA, Viral ,Origin recognition complex - Abstract
Human replication protein A (RPA) is a single-stranded DNA-binding protein that is composed of subunits of 70, 32, and 14 kDa. This heterotrimeric complex is required for multiple processes in DNA metabolism including DNA replication, DNA repair, and recombination. Previous studies have suggested that the 616 amino acid, 70-kDa subunit of RPA (RPA 70) is composed of multiple structural/functional domains. We used a series of N-terminal deletions of RPA70 to define the boundaries of these domains and elucidate their functions. Mutant RPA complexes missing residues 1-168 of RPA70 bound ssDNA with high affinity and supported SV40 replication in vitro. In contrast, deletions extending beyond residue 168 showed a decreased affinity for ssDNA and were inactive in SV40 DNA replication. When residues 1-381 were deleted, the resulting truncated RPA70 was unable to bind ssDNA but still formed a stable complex with the 32- and 14-kDa subunits of RPA. Thus, the C-terminal domain of RPA70 is both necessary and sufficient for RPA complex formation. These data indicate that RPA70 is composed of three functional domains: an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions. For all mutant complexes examined, both phosphorylation of the 32-kDa subunit of RPA and the ability to support T antigen-dependent, origin-dependent DNA unwinding correlated with ssDNA binding activity.
- Published
- 1996
41. Proteolytic Mapping of Human Replication Protein A: Evidence for Multiple Structural Domains and a Conformational Change upon Interaction with Single-Stranded DNA
- Author
-
Xavier V. Gomes, Leigh A. Henricksen, and Marc S. Wold
- Subjects
Base Sequence ,Protein Conformation ,Chemistry ,DNA repair ,Molecular Sequence Data ,DNA replication ,Antibodies, Monoclonal ,DNA, Single-Stranded ,Peptide Mapping ,Biochemistry ,Recombinant Proteins ,DNA-Binding Proteins ,Molecular Weight ,chemistry.chemical_compound ,Protein structure ,SeqA protein domain ,Replication Protein A ,Humans ,Origin recognition complex ,Replication protein A ,DNA ,Sequence Deletion ,Binding domain - Abstract
Replication protein A (RPA) is multisubunit single-stranded DNA-binding protein required for multiple processes in DNA metabolism including DNA replication, DNA repair, and recombination. Human RPA is a stable complex of three subunits of 70, 32, and 14 kDa (RPA70, RPA32, and RPA14, respectively). We examined the structure of both wild-type and mutant forms of human RPA by mapping sites sensitive to proteolytic cleavage. For all three subunits, only a subset of the possible protease cleavage sites was sensitive to digestion. RPA70 was cleaved into multiple fragments of defined lengths. RPA32 was cleaved rapidly to a approximately 28-kDa polypeptide containing the C-terminus that was partially resistant to further digestion. RPA14 was refractory to digestion under the conditions used in these studies. The digestion properties of a complex of RPA32 and RPA14 were similar to those of the native heterotrimeric RPA complex, indicating that the structure of these subunits is similar in both complexes. Epitopes recognized by monoclonal antibodies to RPA70 were mapped, and this information was used to determine the position of individual cleavage events. These studies suggest that RPA70 is composed of at least two structural domains: an approximately 18-kDa N-terminal domain and a approximately 52-kDa C-terminal domain. The N-terminus of RPA70 was not required for single-stranded DNA-binding activity. Multiple changes in the digestion pattern were observed when RPA bound single-stranded DNA: degradation of the approximately 52-kDa domain of RPA70 was inhibited while proteolysis of RPA32 was stimulated. These data indicate that RPA undergoes a conformational change upon binding to single-stranded DNA.
- Published
- 1996
42. Human exonuclease 5 is a novel sliding exonuclease required for genome stability
- Author
-
Marc S. Wold, Rakesh Kumar, Peter M. J. Burgers, Mayank Singh, Tej K. Pandita, and Justin L. Sparks
- Subjects
Genome instability ,Exonuclease ,Exonucleases ,Iron-Sulfur Proteins ,DNA Repair ,DNA repair ,DNA damage ,Ultraviolet Rays ,Molecular Sequence Data ,DNA, Single-Stranded ,Biology ,DNA and Chromosomes ,Biochemistry ,Genomic Instability ,Substrate Specificity ,chemistry.chemical_compound ,Replication Protein A ,Humans ,Amino Acid Sequence ,Molecular Biology ,Replication protein A ,Conserved Sequence ,Chromosome Aberrations ,Sequence Homology, Amino Acid ,Genome, Human ,Mitochondrial genome maintenance ,DNA replication ,Cell Biology ,Molecular biology ,Cell biology ,Cross-Linking Reagents ,chemistry ,biology.protein ,Biocatalysis ,Protein Multimerization ,DNA ,Protein Binding - Abstract
Previously, we characterized Saccharomyces cerevisiae exonuclease 5 (EXO5), which is required for mitochondrial genome maintenance. Here, we identify the human homolog (C1orf176; EXO5) that functions in the repair of nuclear DNA damage. Human EXO5 (hEXO5) contains an iron-sulfur cluster. It is a single-stranded DNA (ssDNA)-specific bidirectional exonuclease with a strong preference for 5′-ends. After loading at an ssDNA end, hEXO5 slides extensively along the ssDNA prior to cutting, hence the designation sliding exonuclease. However, the single-stranded binding protein human replication protein A (hRPA) restricts sliding and enforces a unique, species-specific 5′-directionality onto hEXO5. This specificity is lost with a mutant form of hRPA (hRPA-t11) that fails to interact with hEXO5. hEXO5 localizes to nuclear repair foci in response to DNA damage, and its depletion in human cells leads to an increased sensitivity to DNA-damaging agents, in particular interstrand cross-linking-inducing agents. Depletion of hEXO5 also results in an increase in spontaneous and damage-induced chromosome abnormalities including the frequency of triradial chromosomes, suggesting an additional defect in the resolution of stalled DNA replication forks in hEXO5-depleted cells.
- Published
- 2012
43. Structural Analysis of Human Replication Protein A
- Author
-
Marc S. Wold and Xavier V. Gomes
- Subjects
Protein subunit ,DNA replication ,Cooperativity ,Cell Biology ,Biology ,Biochemistry ,Molecular biology ,Cell biology ,Replication factor C ,Protein structure ,Heterotrimeric G protein ,Origin recognition complex ,Molecular Biology ,Replication protein A - Abstract
Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that is essential for DNA metabolism. Human RPA is composed of subunits of 70, 32, and 14 kDa with intrinsic DNA-binding activity localized to the 616-amino acid, 70-kDa subunit (RPA70). We have made a series of C-terminal deletions to map the functional domains of RPA70. Deletion of the C terminus resulted in polypeptides that were significantly more soluble than RPA70 but were unable to form stable complexes with the other two subunits of RPA. These data suggest that the C-terminal region of RPA70 may be important for complex formation. The DNA-binding domain was localized to a region of RPA70 between residues 1 and 441. A mutant containing residues 1-441 bound oligonucleotides with an intrinsic affinity close to wild-type RPA complex. This mutant also appeared to bind with reduced cooperativity. We conclude that the C terminus of RPA70 and the 32- and 14-kDa subunits are not involved directly with interactions with DNA but may have a role in cooperativity of RPA binding. RPA70 deletion mutants were not able to support DNA replication even in the presence of a complex of the 32- and 14-kDa subunits, suggesting that the heterotrimeric complex is essential for DNA replication. The putative zinc finger in the C terminus of RPA70 is not required for single-stranded DNA-binding activity.
- Published
- 1995
44. In Vitro Analysis of the Role of Replication Protein A (RPA) and RPA Phosphorylation in ATR-mediated Checkpoint Signaling*
- Author
-
Laura A. Lindsey-Boltz, Marc S. Wold, Joyce T. Reardon, and Aziz Sancar
- Subjects
inorganic chemicals ,Cell cycle checkpoint ,DNA Repair ,DNA repair ,DNA, Single-Stranded ,Cell Cycle Proteins ,Ataxia Telangiectasia Mutated Proteins ,Biology ,DNA and Chromosomes ,Protein Serine-Threonine Kinases ,Biochemistry ,complex mixtures ,Cell Line ,Replication Protein A ,Humans ,Protein phosphorylation ,CHEK1 ,Phosphorylation ,Molecular Biology ,Replication protein A ,Cell-Free System ,Cell Biology ,Cell Cycle Checkpoints ,G2-M DNA damage checkpoint ,Molecular biology ,enzymes and coenzymes (carbohydrates) ,Checkpoint Kinase 1 ,Mutation ,bacteria ,biological phenomena, cell phenomena, and immunity ,Tumor Suppressor Protein p53 ,Ataxia telangiectasia and Rad3 related ,Protein Kinases ,Nucleotide excision repair ,Signal Transduction - Abstract
Replication protein A (RPA) plays essential roles in DNA metabolism, including replication, checkpoint, and repair. Recently, we described an in vitro system in which the phosphorylation of human Chk1 kinase by ATR (ataxia telangiectasia mutated and Rad3-related) is dependent on RPA bound to single-stranded DNA. Here, we report that phosphorylation of other ATR targets, p53 and Rad17, has the same requirements and that RPA is also phosphorylated in this system. At high p53 or Rad17 concentrations, RPA phosphorylation is inhibited and, in this system, RPA with phosphomimetic mutations cannot support ATR kinase function, whereas a non-phosphorylatable RPA mutant exhibits full activity. Phosphorylation of these ATR substrates depends on the recruitment of ATR and the substrates by RPA to the RPA-ssDNA complex. Finally, mutant RPAs lacking checkpoint function exhibit essentially normal activity in nucleotide excision repair, revealing RPA separation of function for checkpoint and excision repair.
- Published
- 2012
45. Detection of Posttranslational Modifications of Replication Protein A
- Author
-
Ran Chen, Marc S. Wold, and Cathy S. Hass
- Subjects
DNA Replication ,biology ,Immunoblotting ,DNA replication ,Fluorescent Antibody Technique ,Eukaryotic DNA replication ,complex mixtures ,Molecular biology ,Article ,Cell biology ,DNA-Binding Proteins ,DNA replication factor CDT1 ,enzymes and coenzymes (carbohydrates) ,Replication factor C ,Control of chromosome duplication ,SeqA protein domain ,Replication Protein A ,biology.protein ,Origin recognition complex ,Molecular Biology ,Protein Processing, Post-Translational ,Replication protein A - Abstract
Replication Protein A (RPA) is a single-strand DNA-binding protein that is found in all eukaryotes. RPA is subjected to multiple post-translational modifications including serine- and threonine-phosphorylation, poly-ADP ribosylation and SUMOylation. These modifications are believed to regulate RPA activity through modulating interactions with DNA and partner proteins. This article describes two methods used to detect post-translational modified RPA: immunofluorescence and immmuoblotting.
- Published
- 2012
46. Interactions of human replication protein A with oligonucleotides
- Author
-
Marc S. Wold, Changsoo Kim, and Brian F. Paulus
- Subjects
Hot Temperature ,Polynucleotide 5'-Hydroxyl-Kinase ,Macromolecular Substances ,Ultraviolet Rays ,Cooperativity ,Sodium Chloride ,complex mixtures ,Biochemistry ,Adenosine Triphosphate ,Replication factor C ,SeqA protein domain ,Replication Protein A ,Centrifugation, Density Gradient ,Humans ,Replication protein A ,Binding Sites ,Oligonucleotide ,Chemistry ,Ter protein ,DNA replication ,DNA ,DNA-Binding Proteins ,enzymes and coenzymes (carbohydrates) ,Cross-Linking Reagents ,Spectrometry, Fluorescence ,Oligodeoxyribonucleotides ,Biophysics ,Origin recognition complex ,HeLa Cells - Abstract
Replication protein A (RPA) is a heterotrimeric, single-stranded DNA binding protein that is essential for eukaryotic DNA replication. In order to gain a better understanding of the interactions between RPA and DNA, we have examined the interactions of human RPA with single-stranded oligonucleotides. Our analysis of RPA.DNA complexes demonstrated that RPA binds as a heterotrimer. Stoichiometric binding reactions monitored by fluorescence quenching indicated that the binding site size of human RPA is 30 nucleotides and that between 20-30 nucleotides of DNA directly interact with RPA. The binding of RPA to DNA of different lengths was systematically examined using deoxythymidine-containing oligonucleotides. We found that the binding affinity of RPA for short oligonucleotides was length dependent. The apparent association constant of RPA varied over 200-fold from approximately 7 x 10(7) M-1 for oligo(dT)10 to approximately 1.5 x 10(10) M-1 for oligo(dT)50. Human RPA binds to oligonucleotides with low cooperativity; the cooperativity parameter (omega) for RPA binding was estimated to be approximately 15.
- Published
- 1994
47. Replication protein A mutants lacking phosphorylation sites for p34cdc2 kinase support DNA replication
- Author
-
Marc S. Wold and Leigh k Henricksen
- Subjects
Protein subunit ,DNA replication ,Eukaryotic DNA replication ,Cell Biology ,Biology ,complex mixtures ,Biochemistry ,enzymes and coenzymes (carbohydrates) ,Control of chromosome duplication ,Mutant protein ,Origin recognition complex ,Phosphorylation ,Molecular Biology ,Replication protein A - Abstract
Replication Protein A (RPA) is a multisubunit, single-stranded DNA-binding protein essential for DNA metabolism in eukaryotic cells. The 32-kDa subunit of RPA is phosphorylated in a cell cycle-dependent manner becoming phosphorylated during S phase. It has been postulated that this phosphorylation may regulate the activities of RPA and that the family of p34cdc2 kinases directly catalyzes the phosphorylation of RPA in the cell. We have mutated the two consensus p34cdc2 sites in the 32-kDa subunit of RPA individually and in combination and purified the mutant protein complexes. Mutant RPA with both consensus p34cdc2 sites converted to alanine was not phosphorylated by purified p34cdc2 kinase. Nevertheless, we found that the properties of these RPA mutants were identical to those of the wild-type protein. The mutated RPA proteins had normal single-stranded DNA binding activity and were completely functional for DNA replication. In addition, the mutants became hyperphosphorylated when incubated under DNA replication conditions. These results demonstrate that phosphorylation by p34cdc2 kinase is not essential for RPA function in DNA replication in vitro. Possible roles of RPA phosphorylation on DNA metabolism are discussed.
- Published
- 1994
48. Recombinant replication protein A: expression, complex formation, and functional characterization
- Author
-
Marc S. Wold, Christopher B. Umbricht, and Leigh A. Henricksen
- Subjects
Protein subunit ,DNA replication ,Cell Biology ,Biology ,complex mixtures ,Biochemistry ,Molecular biology ,law.invention ,enzymes and coenzymes (carbohydrates) ,chemistry.chemical_compound ,Plasmid ,Replication factor C ,chemistry ,law ,Recombinant DNA ,Origin recognition complex ,Molecular Biology ,Replication protein A ,DNA - Abstract
Replication protein A (RPA) is a multisubunit, single-stranded DNA-binding protein that is absolutely required for replication of SV40 DNA. The three cDNAs encoding the subunits of human replication protein A (70, 32, and 14 kDa) have been expressed individually and in combination in Escherichia coli. When subunits were expressed individually, appropriately sized polypeptides were synthesized, but were found to be either insoluble or aggregated with other proteins. We examined the interactions between individual RPA subunits by expressing pairs of subunits and determining if they formed stable complexes. Only the 32- and 14-kDa subunits formed a soluble complex when coexpressed. This complex was purified and characterized. The 32-14 kDa subcomplex did not have any effect on DNA replication and was not phosphorylated efficiently in vitro. We believe that the 32.14-kDa subcomplex may be a precursor in the assembly of the complete RPA complex. Coexpression of all three subunits of RPA resulted in a significant portion of each polypeptide forming a soluble complex. We have purified recombinant RPA complex from E. coli and demonstrated that it has properties similar to those of human RPA. Recombinant human RPA has the same subunit composition and the same activities as the authentic complex from human cells. Recombinant human RPA binds single-stranded DNA and is capable of supporting SV40 DNA replication in vitro. In addition, recombinant RPA became phosphorylated when incubated under replication conditions.
- Published
- 1994
49. Inhibition of homologous recombination by DNA-dependent protein kinase requires kinase activity, is titratable, and is modulated by autophosphorylation
- Author
-
Marc S. Wold, Van Dang, Pauline Douglas, Jessica A. Neal, Susan P. Lees-Miller, and Katheryn Meek
- Subjects
Protein subunit ,Cell ,Molecular Sequence Data ,DNA-Activated Protein Kinase ,Biology ,Cell Line ,Catalytic Domain ,medicine ,Animals ,Humans ,DNA Breaks, Double-Stranded ,Amino Acid Sequence ,Kinase activity ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Autophosphorylation ,Cell Biology ,Articles ,Recombinant Proteins ,Non-homologous end joining ,Enzyme Activation ,enzymes and coenzymes (carbohydrates) ,medicine.anatomical_structure ,Biochemistry ,Mutation ,Biocatalysis ,Homologous recombination ,Sequence Alignment - Abstract
How a cell chooses between nonhomologous end joining (NHEJ) and homologous recombination (HR) to repair a double-strand break (DSB) is a central and largely unanswered question. Although there is evidence of competition between HR and NHEJ, because of the DNA-dependent protein kinase (DNA-PK)'s cellular abundance, it seems that there must be more to the repair pathway choice than direct competition. Both a mutational approach and chemical inhibition were utilized to address how DNA-PK affects HR. We find that DNA-PK's ability to repress HR is both titratable and entirely dependent on its enzymatic activity. Still, although requisite, robust enzymatic activity is not sufficient to inhibit HR. Emerging data (including the data presented here) document the functional complexities of DNA-PK's extensive phosphorylations that likely occur on more than 40 sites. Even more, we show here that certain phosphorylations of the DNA-PK large catalytic subunit (DNA-PKcs) clearly promote HR while inhibiting NHEJ, and we conclude that the phosphorylation status of DNA-PK impacts how a cell chooses to repair a DSB.
- Published
- 2011
50. Functional characterization of a cancer causing mutation in human replication protein A
- Author
-
Lokesh Gakhar, Marc S. Wold, and Cathy S. Hass
- Subjects
DNA Replication ,Cancer Research ,DNA Repair ,DNA repair ,Eukaryotic DNA replication ,Biology ,DNA polymerase delta ,Article ,DNA replication factor CDT1 ,Mice ,Replication factor C ,Replication Protein A ,Animals ,Humans ,Phosphorylation ,Molecular Biology ,Replication protein A ,Cell Cycle ,Molecular biology ,enzymes and coenzymes (carbohydrates) ,Oncology ,Gene Knockdown Techniques ,Mutation ,biology.protein ,Origin recognition complex ,DNA mismatch repair ,DNA Damage ,HeLa Cells - Abstract
Replication protein A (RPA) is the primary ssDNA-binding protein in eukaryotes. RPA is essential for DNA replication, repair, and recombination. Mutation of a conserved leucine residue to proline in the high-affinity DNA binding site of RPA (residue L221 in human RPA) has been shown to have defects in DNA repair and a high rate of chromosomal rearrangements in yeast. The homologous mutation in mice was found to be lethal when homozygous and to cause high rates of cancer when heterozygous. To understand the molecular defect causing these phenotypes, we created the homologous mutation in the human RPA1 gene (L221P) and analyzed its properties in cells and in vitro. RPA1(L221P) does not support cell cycle progression when it is the only form of RPA1 in HeLa cells. This phenotype is caused by defects in DNA replication and repair. No phenotype is observed when cells contain both wild-type and L221P forms of RPA1, indicating that L221P is not dominant. Recombinant L221P polypeptide forms a stable complex with the other subunits of RPA, indicating that the mutation does not destabilize the protein; however, the resulting complex has dramatically reduced ssDNA binding activity and cannot support SV40 DNA replication in vitro. These findings indicate that in mammals, the L221P mutation causes a defect in ssDNA binding and a nonfunctional protein complex. This suggests that haploinsufficiency of RPA causes an increase in the levels of DNA damage and in the incidence of cancer. Mol Cancer Res; 8(7); 1017–26. ©2010 AACR.
- Published
- 2010
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