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This study evaluated in vitro the effects of calcium gluconate (CaGlu), sodium fluoride (NaF), sodium hexametaphosphate (HMP), and NaF/TMP added to a 35% hydrogen peroxide (HEnamel discs/bovine dentin (n = 150) were divided according to the bleaching gel: 35% HAll bleaching gels showed significant color changes after treatment (p 0.001). ΔE and ΔWIIt is possible to conclude that the addition of NaF/HMP to the in-office bleaching agent did not interfere with the bleaching efficacy and reduced enamel demineralization and HThe association of NaF/HMP to the bleaching gel can be used as a novel approach for minimizing the adverse effects of H

Objectives To evaluate in vitro the effects of sodium fluoride (F) and nano-sized sodium trimetaphosphate (TMPnano) added to a 35% hydrogen peroxide (H2O2) bleaching gel on the color alteration, enamel mechanical and morphological properties, and H2O2 transamelodentinal diffusion. Materials and Methods Bovine enamel/dentin discs (n = 180) were divided according to the bleaching gel: 35% H2O2 (HP); 35% H2O2 + 0.1% F (HP/F); 35% H2O2 + 1% TMPnano (HP/TMPnano); 35% H2O2 + 0.1% F + 1% TMPnano (HP/F/TMPnano) and 35% H2O2 + 2% calcium gluconate (HP/Ca). The gels were applied 3 times by 40 min; once each 7-day. Color alteration (ΔE and ΔE00), whitening index (ΔWID), surface (SH) and cross-sectional hardness (ΔKHN), surface roughness (Ra), and transamelodentinal diffusion were determined. Enamel surfaces were evaluated by Scanning Electron Microscopy (SEM) and X-ray Dispersive Energy (EDX). Data were submitted to ANOVA, followed by the Student-Newman-Keuls test (p 2O2 diffusion were lower for HP/F/TMPnano; the HP and HP/Ca groups presented the highest values (p 2O2 diffusion, and morphological changes. Clinical Relevance: Whitening gels containing F/TMPnano can be used as a new strategy to increase safety and maintain clinical performance.

Temporary prosthesis protects the oral tissues, in addition to providing aesthetic look and masticatory function until a definitive prosthesis is manufactured.To evaluate the effect of glaze and 0.12% chlorhexidine (CHX) on the physical and mechanical properties of bis-acryl, and to evaluate the antimicrobial efficacy of CHX.Eighty specimens of bis-acryl resin were made. Over 40 of them the glaze was applied. One specimen with and 1 specimen without glaze were placed in niches of an appliance manufactured for each patient. Each of the 20 volunteers received 2 devices. Initially, the volunteers used one device and treated it with sucrose for 7 days (control), and later they used the other device and treated it with sucrose and CHX for 7 days (test). Color, microhardness, roughness, surface energy, and insoluble extracellular polysaccharides (EPS) tests were performed. All results were submitted to the Tukey's test, with the exception of the EPS results, which were submitted to the Student's t test.The ΔE00 of the unglazed control group was significantly higher than that of the unglazed test group. In all groups, a significant decrease in microhardness occurred over time. At both times, the glaze significantly increased the microhardness of the specimens (in all the glazed groups). At the final time, the test glaze group showed significantly higher microhardness compared with the control glaze group. Roughness in the groups without glaze increased significantly with CHX treatment over time. At both times, the glaze generated a significant reduction in roughness in the control and test groups. There was a significant reduction in surface energy over time in all groups. In most comparisons, the glazed groups showed significantly higher surface energy values compared with the unglazed control group. At the final time point, the unglazed test group showed a significantly higher surface energy value than the unglazed control group; and the glazed test group showed a significantly higher surface energy value compared with the glazed control group. The resins that received CHX had a significantly lower amount of biofilm.Color values were clinically acceptable in all tested groups. At both time points, the roughness values were clinically acceptable only in the glazed groups. Glaze increased the microhardness of the specimens. Microhardness and surface energy were reduced over time in all groups. Chlorhexidine can help prevent microhardness degradation. Glaze and CHX can increase surface energy. Chlorhexidine reduced the amount of bacterial biofilm.

Made available in DSpace on 2022-04-29T08:33:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Purpose: To evaluate the type and the amount of fluoridated dentifrice applied on children’s toothbrushes by parents/guardians according to descriptions typically recommended by scientific societies, as well as to assess the influence of demographic and socioeconomic variables on dentifrice use. Methods: Parents/guardians of children (0–7 years old; n = 306; convenience sample) attending vaccination centres from Araçatuba (Brazil), answered to a structured questionnaire comprising items related to interviewees’ education, child’s age, gender, brushing habits and use of fluoridated dentifrice. The amount of toothpaste used by children during toothbrushing was estimated using a portable scale. Similarly, the interviewees were requested to apply dentifrices on toothbrushes according to eight descriptions, ranging from “smear” to “all bristles”, following a random sequence. Data were submitted to Mann–Whitney’s, Kruskal–Wallis’ and Friedman’s tests, and Spearman’s correlation coefficient (p < 0.05). Results: The type of toothpaste and the amount of product used at home were not affected by the respondents’ educational level or family income. However, child’s age was significantly correlated with the amount of toothpaste placed on the toothbrush (r = 0.324, p < 0.001). Also, the amount of toothpaste placed on the toothbrush increased according to what would be expected from the descriptions, although wide variations were observed within each description, with large interquartile and overall ranges. Conclusion: The amount and the type of dentifrice used by children were influenced by their age, while parents/caregivers’ interpretation on verbal instructions regarding appropriate dentifrice quantities varied widely. This reinforces the need for educative measures on the appropriate use by dentifrices by children. Department of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP), Rua José Bonifácio 1193 Graduate Program in Dentistry (GPD Master’s Degree) University of Western São Paulo (UNOESTE) Department of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University (UNESP), Rua José Bonifácio 1193

A precoce intervenção do cirurgião dentista em casos de impacção dentária, diminui as chances de um tratamento mais complexo posteriormente, e aumentam a estética e função da cavidade oral. O trabalho tem como finalidade, apresentar a ulectomia como procedimento cirúrgico de escolha, em um caso clínico com impacção dentária por fibrose gengival. Paciente do gênero feminino, 8 anos de idade, compareceu a clínica de Odontopediatria da Faculdade Morgana Potrich – FAMP, tendo como queixa principal a ausência dos dentes da frente. Realizado o exame intra-oral constatamos que se tratava da ausência dos dentes 11 e 21 e através do exame radiográfico, foi confirmado que os dentes estavam formados 2/3 da raiz completa, sendo o suficiente para erupcionarem e ocuparem seus lugares na arcada dentária, no entanto, somente a força eruptiva não era suficiente para romper a barreira fibrótica. O plano de tratamento estabelecido foi a exérese do tecido fibroso na região incisal dos dois dentes, possibilitando passagem para se posicionarem em oclusão. Desta forma, o tratamento evitou maiores transtornos e proporcionou maior qualidade de vida a paciente, devolvendo a função e estética.

A prática de alimentação, além de fatores secundários, recebe destaque na etiologia da Cárie na Primeira Infância (CPI). Essa doença é definida como a presença de uma ou mais superfícies dentárias cariadas, perdidas ou obturadas em crianças com idade inferior a 6 anos, tendo o presente estudo como objetivo avaliar as práticas alimentares na infância, bem como fatores secundários, e sua relação com a cárie dentária de pacientes atendidos na Clínica de Odontopediatria I e II da Universidade de Ribeirão Preto. Inicialmente, este estudo foi submetido ao Comitê de Ética em Pesquisa da UNAERP, o qual foi aprovado, e posteriormente, foi realizada a coleta de dados. Para a avalição, foi aplicado um questionário estruturado abordando alguns itens sobre hábitos da criança e seu responsável: Alimentação; Escovação; Erupção dentária; Dieta; Avaliação comportamental; Presença de lesões cariosas; entre outros. Após os dados serem tabulados e analisados de forma descritiva, foi possível evidenciar que das 34 crianças participantes do estudo, 97% realizaram amamentação natural (peito). A alimentação industrializada iniciou-se em 53% dos participantes entre 1 e 2 anos; 59% escovavam os dentes 3x/dia ou mais, sendo que 35% não recebiam auxílio. 97% utilizavam pasta de dente fluoretada. A maioria (65%) relatou não usar o fio dental; 53% tiveram o nascimento do primeiro dente antes dos 6 meses de idade e 38% entre 6 meses e 1 ano. 59% consomem doces 1x/dia, sendo que 79% nunca foram ao nutricionista. Ao exame clínico, 24% apresentaram lesão de mancha branca (75% ativa). É possível concluir que hábitos nocivos à saúde bucal de crianças pré-escolares podem resultar em consequências negativas para os elementos dentários.

Ulectomia é um procedimento cirúrgico que consiste na remoção dos tecidos que interrompem a erupção dentária. O presente estudo tem como proposição relatar um caso clínico de ulectomia em paciente pediátrico na região do dente 22. Paciente de 8 anos, do gênero masculino, compareceu a clínica de Odontopediatria da Universidade de Ribeirão Preto (UNAERP) para tratamento odontológico. Ao exame clínico intra-bucal, observou-se aumento volumétrico na região vestibular do dente 22 que ainda não havia erupcionado. A mãe relatou que o filho não apresentou nenhum tipo de trauma naquela região. Após exame radiográfico, observou-se a presença do dente 22, o qual se encontrava em posição intra-óssea. Assim, foi indicado como forma de tratamento a ulectomia por tratar-se de um procedimento simples e minimamente invasivo. Devido à criança não apresentar bom comportamento diante do exame clínico e radiográfico, optou-se executar inicialmente, sessões de condicionamento para que a seguir, fosse efetuado o procedimento cirúrgico. Após três semanas, foi realizada a cirurgia. Inicialmente foi realizada uma incisão elíptica, onde foi possível a visualização da região incisal do dente 22. Foi realizado o acompanhamento clínico após 7, 15, 45 e 180 dias, sendo possível observar a erupção do dente 22. Nos casos de impactações dentárias, há a necessidade de estabelecer a relação com seu fator etiológico para um correto planejamento. Conclui-se que o tratamento realizado proporcionou melhora estética e fonética para o paciente, assim como influenciou no convívio social do mesmo.

O objetivo do presente estudo foi relatar um caso clínico de subluxação, seguida de trauma recorrente, bem como complicações clínicas, radiográficas e plano de tratamento. Paciente do gênero feminino, 4 anos e 5 meses de idade, compareceu à clínica de Odontopediatria da Faculdade de Odontologia de Araçatuba (FOA/UNESP), Brasil, cuja mãe relatava o aparecimento de “bolinha” na região superior do dente 51 e 61. Durante anamnese, a mãe relatou que a criança havia caído há 12 meses e batido o dente 51 apresentando sangramento, leve mobilidade e fratura corono-esmalte, e que segundo a odontopediatra que atendeu a criança no momento do trauma, se tratava de subluxação, e há 2 meses houve trauma recorrente. Ao exame clínico observou-se alteração na coloração do dente 51 associado à fratura corono-esmalte, fístula e mobilidade. Já no dente 61, presença de fístula e mobilidade. A análise radiográfica mostrou reabsorção radicular extensa do dente 51 e 61, ambos com rompimento da cripta óssea do germe dos dentes permanentes 11 e 21, associado a lesão periapical, denotando necrose pulpar. O plano de tratamento instituído foi a exodontia dos dentes 51 e 61, seguido pelo acompanhamento clínico e radiográfico. Embora a subluxação não seja caracterizada como um trauma severo, é importante informar aos pais/responsáveis pela criança sobre ao acompanhamento longitudinal, uma vez que no futuro consequências mais severas podem ocorrer, e dessa forma quando diagnosticada precocemente, a alteração, é passível de tratamento efetivo e satisfatório, levando à preservação do dente até o momento de sua esfoliação.

Talon cusp is a rare dental anomaly that occurs more frequently in maxillary incisors and is defined as a projection in the area of the cincture of anterior teeth that extends from the cemento-enamel junction or the region of the cincture, following towards the incisal face. An eight-year-old female patient was referred to the University of Ribeirão Preto Pediatric Dentistry Clinic (UNAERP) with suspicion of a supernumerary tooth on the palatal surface of tooth 12. During clinical and radiographic examination she found the presence of a conical-shaped enamel projection that presented a communication with the tooth 12 cincture and was thus classified as a talon cusp. Initially, prophylaxis and removal of the accumulated bacterial plaque were performed, verification of premature contact with paper for the joint and wear with a spherical diamond tip. FluroShield sealant was then applied following an adhesive protocol indicated by the manufacturer. After the necessary occlusal adjustments were made. 30 days later, the patient returned for clinical follow-up, reconversion and repolishing of the sealant. The talon cusp was diagnosed as Type I because it is morphologically well delineated and protrudes prominently from the palatal surface and extends at least halfway from the cemento-enamel junction to the incisal edge. It is recommended to seal the deep grooves to prevent caries. We can conclude that a rigorous clinical and radiographic examination leading to an early and accurate diagnosis is of extreme importance for the adoption of an adequate treatment.

The aim of this study was to report a clinical case of a natural tooth found in a 1-day-old newborn, its clinical implications and proper management. The female patient was referred by a pediatrician who contacted the University of Ribeirão Preto Pediatric Dentistry Clinic (UNAERP, Ribeirão Preto, Brazil), informing that the patient with 40 hours of life, still in the maternity ward, had a foreign body in the oral cavity since birth, which was bothering the mother at the time of breastfeeding. At clinical examination, the presence of a Christmas tooth with grade 3 mobility was observed. After one month of life, it was possible to perform a radiographic examination, which resulted in the definitive diagnosis of the Christmas tooth, and the proposed treatment was the surgical removal of the dental element and subsequent follow-up. Prior to surgical removal, the biofilm present on the coronary surface was aseptically collected for microbiological analysis consisting of isolating the bacteria in different culture media and identifying them by sequencing 16S rRNA. The extracted tooth was scanned in high-resolution microtomography, reconstructed, and qualitatively analyzed. Microbiological analyses showed the presence of two bacterial species Streptococcus salivarius and Staphylococcus hominis, both species considered saprophytes, being part of the oral microbiota. The knowledge of this anomaly and the training of dentists to perform an accurate diagnosis, as well as the importance of multidisciplinary work, are of extreme relevance.

This study aimed to evaluate the surface (γs) and interaction (ΔGiwi) free energy and calcium (Ca2+) and phosphate (PO43−) adsorption to dental enamel treated with sodium trimetaphosphate (TMP) or calcium glycerophosphate (CaGP) that had or had not been exposed to CaPO4-containing solutions. Bovine enamel blocks (n = 192; 24 blocks/group) were treated (2 mL/block; 2 min) with TMP (0%, 1%, 3%, and 9%) and CaGP (0, 0.25, 0.5, and 1%) or exposed to a CaPO4-containing solution. The adsorption of these compounds by enamel was assessed before and after treatment. γs and ΔGiwi and their apolar (γsLW and ΔGiwiLW) and polar (γsAB and ΔGiwiAB) components and acid-base interactions (γs+/γs−) were determined by the contact angles. The data were subjected to ANOVA, followed by the Student-Newman-Keuls test (p < 0.05). The adsorption of TMP was dose dependent (p < 0.001), and it reduced γs and γsAB and increased ΔGiwiAB (ΔGiwi > 0) and γs− when compared with the group without TMP (p < 0.001). The immersion in CaPO4-containing solution increased γs and γsAB and reduced ΔGiwiAB (ΔGiwi > 0) and γs− (p < 0.001). There was a correlation between the adsorption of TMP and Ca2+ (r = 0.916; p < 0.001) and PO43− (r = 0.899; p < 0.001). The adsorption of CaGP on the enamel was dose dependent (p < 0.001), reducing γs, ΔGiwiAB (ΔGiwi < 0), γsLW, and γs− when compared to the group without CaGP (p < 0.001). When exposed to the CaPO4-containing solution, there was an increase in ΔGiwiAB (ΔGiwi > 0), γsLW, and γs− and a decrease in γsAB (p < 0.001) without adsorption of Ca2+ by enamel. It may be concluded that TMP and CaGP were adsorbed onto the enamel, producing hydrophilic and hydrophobic surfaces, respectively. TMP produces electron donor sites that induce Ca2+ adsorption, while CaGP releases Ca2+ into the medium.

O freio lingual é uma estrutura anatômica que desenvolve importante papel no ato da sucção, fala e alimentação. A anquiloglossia constitui uma anomalia congênita sendo caracterizada por alteração no freio da língua que resulta em limitações dos movimentos dessa estrutura, podendo ser identificada em diferentes faixas etárias. O presente trabalho tem como proposição relatar um caso clínico de frenectomia lingual em paciente pediátrico, bem como, a conduta clínica e cirúrgica para o presente caso. Paciente, GMF, do gênero masculino, 6 anos de idade, compareceu a Clínica de Odontopediatria da Faculdade de Odontologia de Araçatuba, FOA/UNESP, Brasil, para avaliação do freio lingual, o qual segundo a mãe o incomodava para falar e se alimentar de forma correta. O paciente foi submetido ao exame clínico, sendo verificada a presença de baixa inserção do freio lingual e dificuldade de pronúncia de palavras. Diante das características clínicas, o diagnóstico foi de anquiloglossia, e o plano de tratamento instituído foi a frenectomia lingual. O procedimento cirúrgico contou com as seguintes etapas: anestesia tópica, associada à anestesia terminal infiltrativa e secção do freio lingual com a utilização de tesoura de ponta reta. Após a realização do procedimento cirúrgico pode-se observar melhor mobilidade da língua do paciente. Conclui-se que a frenectomia mostrou-se eficiente para melhorar a postura e mobilidade da língua, funções orais e da comunicação. Descritores: Odontopediatria; Freio Lingual; Criança. Referências Corrêa M, Alvarez J, Corrêa F, Azevedo de G, Bonini V, Alves F. Anquiloglosia y amamantamiento: Revisión y reporte de caso. Rev estomatol Hered. 2008;18(2):121-27. Kotlow LA. Ankyloglossia (tongue-tie): a diagnostic and treatment quandary. Quintessence Int. 1999;30(4):259-62. Brito SF, Marchesan IQ, Bosco CM, Carrilho ACA, Rehder MI. Frênulo lingual: classificação e conduta segundo ótica fonoaudiológica, odontológica e otorrinolaringológica. Rev CEFAC. 2008;10(3):343-51. Braga LAS, Pantuzzo CA, Motta AR. Prevalência de alterações no frênulo lingual e suas implicações na fala de escolares. Rev CEFAC. 2009;11(Suppl 3):378-90. Guedes PAC. Odontopediatria. 7. ed. São Paulo: Santos; 2003. Borsatto MC, Torres CP, Assed S. Cirurgia em Odontopediatria. In: Sada S. Odontopediatria: bases científicas para a prática clínica/SadaAssed. São Paulo: Artes Médicas; 2005;239-89. Martinelli R, Marchesan I, Berretin FG. Estudo longitudinal das características anatômicas do frênulo lingual comparado com afirmações da literatura. Rev CEFAC. 2014;16:1202-07. Fujinaga C, Chaves J, Karkon I, Klossowski D, Silva F, Rodrigues A. Frênulo lingual e aleitamento materno: Estudo Descritivo. Audiol Commun Res. 2017;22:e1762. Puricelli E, Ponzoni D. Aspectos da cirurgia e traumatologia bucomaxi-lofacial em odontopediatria. In: Toledo OA. Odontopediatria: fundamentos para a prática clínica. Rio de Janeiro: MedBook; 2012;329-48. Ballard JL, Auer CE, Khoury JC. Ankyloglossia: assessment, incidence, and effect of frenuloplasty on the breastfeeding dyad. Pediatrics. 2002;110(5):e63. Segal LM, Stephenson R, Dawes M, Feldman P. Prevalence, diagnosis, and treatment of ankyloglossia: methodologic review. Can Fam Physician. 2007;53(6):1027-33. Baldani MH, Lopes CML, Scheidt WA. Prevalence of oral alterations in infants seen at the public pediatric dental clinics from Ponta Grossa - PR, Brazil. Pesqui. Odontol. Bras. 2001;15(4):302-7. Marchesan I, Rehder M, Oliveira L, Lanboglia R, Araujo T, Costa M, Martinelli R. Incidência de alterações de frênulo da língua em uma população de crianças de 1ª a 3ª série de ensino fundamental. 16° Congresso Brasileiro de Fonoaudiologia., Campos do Jordão-SP. 2008. Martinelli V, Martinelli R, Marchesan I, Berretin FG, Souza S. Elaboração e desenvolvimento de um website sobre o teste da linguinha. Rev CEFAC. 2017;19(2):260-64. Friggi MNP, Orsi RM, Chelotti A. Técnica cirúrgica pediátrica – frenectomia lingual. JBP j bras odontopediatr odontol bebê. 1998;1(3):101-15. Marquezan IQ, Martinelli RLC, Gusmão RJ. Frênulo lingual: modificações após frenectomia. J Soc Bras Fonoaudiol 2012;24(4):409-12. Veyssiere A, Kun-Darbois JD, Paulus C, Chatellier A, Caillot A, Bénateau H. Diagnostic et prise en charge de l'ankyloglossie chez le jeune enfant [Diagnosis and management of ankyloglossia in young children]. Rev Stomatol Chir Maxillofac Chir Orale. 2015;116(4):215-20. Almeida RR, Garib DG, Almeida-Pedrin RR, Almeida MR, Pinzan A, Junqueira MHZ. Diastemas interincisivos centrais superiores: quando e como intervir? R Dental Press Ortodon Ortop Facial. 2004;9(3):137-56. Almeida LF. Freio labial superior: aspectos gerais e tratamentos [monografia]. Cuiabá: Curso de Especialização em Periodontia, Centro de Aperfeiçoamento e Especialização Sinodonto (caes - Mt); 2006. 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Made available in DSpace on 2020-12-12T02:42:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Objective: This study evaluated the effects of calcium gluconate (CaGlu), sodium fluoride (NaF), sodium trimetaphosphate (TMP), and NaF/TMP added to a 35% hydrogen peroxide (HP) bleaching gel for the reduction in enamel demineralization in vitro, with and without the use of a fluoridated dentifrice. Design: Enamel blocks (n = 100) were obtained from bovine incisors (n = 200) after flattening and subjected to initial surface hardness (SH) analysis. The blocks were divided according to the bleaching gel (35% HP; 35% HP + 0.05% NaF; 35% HP + 0.25% TMP; 35% HP + 0.05% NaF + 0.25% TMP; 35% HP + 2% CaGlu) and were treated with ether non-fluoridated or fluoridated (1100 ppm) dentifrice. The bleaching gels were applied thrice (40 min/session) at the intervals of 7 days between each application. After 21 days, the final SH for the calculation of the percentage of SH loss (%SH) and cross-sectional hardness for the evaluation of the integrated hardness area (IH) were determined. Results: Bleaching containing HP + NaF + TMP presented lowest %SH (p < 0.001), regardless of the dentifrice used. HP + NaF + TMP bleaching gel led to lower subsurface enamel mineral loss (IH) compared to the other groups (p < 0.001), and these did not differ from each other (p > 0.05). Daily use of fluoride dentifrice led to higher IH values (p < 0.001), regardless of the bleaching gels. Conclusion: The addition of NaF/TMP to a 35% HP bleaching gel remarkably reduced the mineral loss compared to the cases of the other bleaching gels, regardless of dentifrice. Clinical relevance: The association of TMP/NaF can be used as a strategy for reducing mineral loss during the bleaching procedure, even without the daily use of fluoride dentifrice. School of Dentistry Department of Preventive and Restorative Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193 Department of Dentistry University of Ribeirão Preto (UNAERP), Ribeirão Preto, Avenida Costábile Romano, 2201 School of Dentistry Department of Preventive and Restorative Dentistry São Paulo State University (UNESP), Rua José Bonifácio, 1193

Made available in DSpace on 2020-12-12T02:46:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 The present study evaluated fluoride (F) concentrations in saliva of toddlers after brushing with dentifrices containing different F concentrations, applied in different quantities, and estimated F intake from toothbrushing. The study comprised a double-blind, crossover protocol, in which toddlers (n = 18, 2-3 years old) were randomly assigned into six groups, according to possible combinations of dentifrices (0/550/1,100 ppm F, as NaF) and amounts (rice grain, pea size, and transverse technique). Volunteers used a F-free dentifrice during 1 week. On the 7th day, saliva samples were collected before (baseline), and at 5/15/30/60 min after toothbrushing. All dentifrice expectorated after brushing was collected. F concentrations (saliva and expectorate) were determined with an ion-specific electrode. Data were submitted to ANOVA or Kruskal-Wallis test, followed by Fisher's LSD or Student-Newman-Keuls' tests (p

Made available in DSpace on 2020-12-12T02:34:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 This in vitro study evaluated the protective effect of fluoride varnishes containing sodium trimetaphosphate (TMP) against dentin erosion and abrasion. Specimens of coronal dentin were divided into: Placebo, 2.5% NaF, 5% NaF, 2.5% NaF + 5% TMP, and 5% NaF + 5% TMP groups (n =24/group). After single application of the varnishes, the samples were immersed in citric acid (0.05 mol/L, pH = 3.2, 5 min) followed or not by brushing, and the dentin wear was assessed after 5 days. Varnishes containing fluoride + TMP led to the lowest wear. TMP varnishes showed a superior effect against dentin erosive wear. Department of Pediatric Dentistry and Public Health School of Dentistry Sao Paulo State University (UNESP), Rua Jose Bonifacio 1193 Centro de Ensino Superior Rezende and Potrich FAMA-Faculdade Mineirense Mineiros Department of Pediatric Dentistry and Public Health School of Dentistry Sao Paulo State University (UNESP), Rua Jose Bonifacio 1193

Made available in DSpace on 2019-10-06T15:39:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-05-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Objective: To evaluate the antimicrobial/antibiofilm and mechanical properties, and the effect on enamel demineralization of a resin-modified GIC (RMGIC) containing CHX and nano-sized sodium trimetaphosphate (TMP). Methods: RMGIC was associated with CHX (1.25 or 2.5%) and/or TMP (7 or 14%). Antimicrobial and antibiofilm activity were assessed using agar diffusion test and evaluation of biofilm metabolism, respectively. In addition, fluoride (F) and TMP releases as well as the diametral tensile (DTS) and compressive (CS) strength were determined. The percentage of mineral loss (%SH), integrated loss of subsurface hardness (ΔKHN) and enamel F concentrations were also evaluated. Results: RMGICs containing CHX associated or not with TMP presented higher inhibition zones and effect on S. mutans biofilm. A reduction on CS was observed only for RMGIC + 2.5%CHX and on DTS for RMGIC + 2.5%CHX + 14%TMP. The highest F and TMP releases and lowest %SH and ΔKHN values were detected for RMGIC + 1.25%CHX + 14%TMP and RMGIC + 2.5%CHX + 14%TMP. Higher enamel F concentrations were observed for TMP groups. Conclusion: 1.25%CHX and 14%TMP increased antimicrobial/antibiofilm action and the ability to prevent enamel demineralization, with minimal effect on the mechanical properties of RMGIC. Clinical significance: RMGIC containing CHX and TMP is an alternative material for patients at high risk for dental caries and can be indicated for low-stress regions or provisional restorations. Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic São Paulo State University (UNESP) School of Dentistry Department of Chemistry Federal University of São Carlos Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of Dentistry Department of Surgery and Integrated Clinic São Paulo State University (UNESP) School of Dentistry

The study evaluated the effect of solutions containing fluoride (F) and/or sodium trimetaphosphate (TMP) and F/TMP on the inhibition of MMP-2 and MMP-9, and on dentin remineralization in vitro.Bovine root dentin blocks were prepared, and caries-like lesions were induced in two thirds of the surface. Blocks were then randomly divided into 13 groups/solutions (n = 10): Placebo; 0.3 %, 1 % and 3 % NaOH-hydrolyzed TMP; 0.3 %, 1 % and 3 % TMP; 250, 500 and 1100 ppm F; 250 ppm F + 0.3 % TMP; 500 ppm F + 1 % TMP and 1100 ppm F + 3 % TMP. One third of each specimen was treated with the respective solutions in pH-cycling. The mineral concentration (gF/TMP association led to less mineral loss in the deeper region of the lesion and reduced the depth of lesions when compared to its counterpart without TMP (p 0.001). 3 % TMP (hydrolyzed or not), 500 ppm F and 1100 ppm F completely inhibited MMP-2 activity, while for MMP-9 such effects were only achieved by treatment with 1100 ppm F + 3 % TMP.Treatment with 1100 ppm F + 3 % TMP fully inhibits the gelatinolytic activity of MMPs-2 and - 9 and shows greater remineralizing capacity in artificial caries lesions in dentin. However, hydrolyzing TMP does not improve its anti-proteolytic activity and its remineralizing capacity.

The aim of this study were to produce a multifunctional nanocomposite combining silver nanoaparticles (Ag), sodium trimetaphosphate (TMP) and fluoride (F), to investigate its effect on dental enamel demineralization and on biofilms of Streptococcus mutans and Candida albicans.Bovine enamel blocks were submitted to five pH cycles and treated 2x/day with 100 ppm F, 225 ppm F, 100 ppm F + 0.2%TMP or 100 ppm F + 0.2%TMP+10% Ag (100F/TMP/Ag). Next, surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), enamel fluoride (F) and calcium (Ca) concentration were determined. Biofilms from single and dual species of S. mutans and C. albicans were treated with 100F/TMP/Ag, Ag or chlorhexidine gluconate for 24 h. The antibiofilm effect was evaluated by colony-forming unit counting and Scanning Electron Microscopy.The nanocomposite reduced 43.0% of %SH and was similar with samples treated with 225F, 100F/TMP and 100/TMP/Ag. The attribute of F and/or TMP in reducing ΔKHN in 5-20 μm was not affected by the addiction of Ag (110F = 225F = 100F/TMP = 100F/TMP/AgNegative Control). Further, 100F/TMP/Ag strongly reduced viable cells of S. mutans in dual biofilms (∼5 logThe 100F/TMP/F promoted a protective effect against enamel demineralization and was able to significantly inhibit the growth of biofilms of S. mutans and C. albicans.The focus on prevention and non-invasive dental treatment is the most effective and least costly way to improve the population's oral health conditions. We present a nanocomposite for a multiple approach in prevention of caries.

Made available in DSpace on 2022-04-29T08:30:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01 Objective: To evaluate the effect of low-fluoride (F−) toothpaste and sodium trimetaphosphate (TMP) associated with xylitol and erythritol (XE) on enamel demineralization and biofilm composition. Methods: This crossover double-blind in situ study consisted of five phases (seven days each), in which 14 volunteers wore oral appliances containing four enamel bovine blocks. The cariogenic challenge was performed by exposure to a 30% sucrose solution (6x/day). The toothpaste treatments (3x/day) were as follows: placebo (no F−/TMP/XE); 200 ppm F− (NaF) (200F); 1,100 ppm F− (1100F); 16% Xylitol and 4% Erythritol (XE); and 200 ppm F−, 0.2% TMP, 16% xylitol, and 4% erythritol (200F-TMP-XE). Percentage of surface hardness loss (%SH) and integrated loss of subsurface hardness (ΔKHN), and calcium (Ca2+), phosphate (PO43−), and F− on enamel and biofilm were determined; as well as insoluble extracellular polysaccharide (EPS). Results: XE and 1100F groups showed no significant difference for %SH and ΔKHN values (p = 0.220 and p = 0.886), and the 200F-TMP-XE group had the lowest mineral loss (p < 0.001). Ca2+ and PO43− in the enamel showed the highest values (p < 0.001) for the 200F-TMP-XE group. Higher values of F− in the enamel and biofilm were observed for the 1100F group (p < 0.001). There was no difference for Ca2+ (p = 1.00) and EPS (p =0.918) values between XE and 200-TMP-XE groups in the biofilm, but their values were higher and lower than the 1100F (p = 0.002 and p = 0.029), respectively. Conclusions: 200F-TMP-XE promoted a greater protective effect against enamel demineralization and significantly affected the composition of biofilm formed in situ compared to 1100F toothpaste. Clinical Significance: Low-F− toothpaste containing TMP and polyols can be considered an effective and safe measure to improve the oral health of individuals, especially patients with high caries activity. State University of Londrina School of Dentistry Department of Oral Medicine and Children's Dentistry, Rodovia Celso Garcia Cid, PR 445 Km 380 São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry Rua, José Bonifácio 1193 Northwestern University Feinberg School of Medicine Ann and Robert Lurie Children's Hospital, 420 E Superior St São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry Rua, José Bonifácio 1193

Made available in DSpace on 2021-06-25T10:34:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01 Objective: To evaluate the effect of treatment with fluoridated toothpaste supplemented with a combination of sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®) on the demineralization of dental enamel. Methods: Bovine enamel blocks selected by initial surface hardness (SHi) were randomly allocated into six groups (n = 12), according to the test toothpastes: (1) without F-TMP-MI Paste Plus® (Placebo); (2) 1100 ppm F (1100F); (3) MI Paste Plus®; (4) 1100F + MI Paste Plus® (1100F-MI Paste Plus), (5) 1100F + 3 % TMP (1100F-TMP); and (6) 1100F-TMP + MI Paste Plus® (1100F-TMP-MI Paste Plus). Blocks were treated two times per day with slurries of toothpaste (1 min), and groups 4 and 6 received an application of MI Paste Plus (3 min). Next, the samples were subjected to five pH cycles (demineralizing/remineralizing solutions) at 37 °C, to produce subsurface enamel lesions.Thereafter, the blocks were maintained for 2 days in fresh remineralizing solution. After pH cycling, the following were obtained: percentage of surface hardness loss (%SH); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM); scanning electron microscopy (SEM); and fluoride (F), calcium (Ca), and phosphorus (P) in the enamel. The data were subjected to ANOVA (1-criterion), followed by the Student–Newman–Keuls test (p < 0.001). Results: The 1100F-TMP-MI Paste Plus group showed better results for SHR, ΔKHN, and PLM (p < 0.001). The F concentration was similar among all groups (p > 0.001). The 1100F-TMP-MI Paste Plus group showed the highest concentration of Ca and P in the enamel (p < 0.001). Conclusion: The application of 1100F-TMP-MI Paste Plus promoted a higher inhibitory effect against enamel demineralization. Clinical significance: The combination of treatments with F, TMP, and MI Paste Plus® can be an effective alternative to improve the oral health of individuals, especially those with high activity of dental caries and at high risk for its development. Department of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry Araçatuba, Rua José Bonifácio 1193 Department of Materials Engineering Federal University of São Carlos - UFSCar Feinberg School of Medicine Ann and Robert Lurie Children’s Hospital Northwestern University School of Dentistry University of Ribeirão Preto—UNAERP Department of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry Araçatuba, Rua José Bonifácio 1193

Made available in DSpace on 2021-06-25T10:18:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 ASCRS Research Foundation Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) This study evaluated the effects of calcium gluconate (CaGlu), sodium fluoride (NaF), sodium trimetaphosphate (TMP), and NaF/TMP when added to a 35% hydrogen peroxide (H2O2) bleaching gel on the color change, enamel hardness, penetration and cytotoxicity trans-amelodentinal. Bovine enamel/dentin disks (n = 288) were divided according to the bleaching gel: 35% H2O2; 35% H2O2 + 0.05% NaF; 35% H2O2 + 0.25% TMP; 35% H2O2 + 0.05% NaF + 0.25% TMP; 35% H2O2 + 0.1% NaF + 1% TMP and 35% H2O2 + 2% CaGlu. The bleaching gels were applied thrice (40 min/session) at 7-day intervals between each application. Then, the color change, percentage of surface hardness loss (%SH), cross-sectional hardness (ΔKHN), trans-amelodentinal penetration of H2O2, cell viability and morphology (MDPC-23 odontoblast-like cells), alkaline phosphatase activity (ALP) and deposition of mineralization nodules were determined. The data were submitted to ANOVA, followed by the Student-Newman-Keuls test (p < 0.05). All bleaching gels showed significant color changes after treatment (p < 0.001). Mineral loss (%SH and ΔKHN) and H2O2 penetration were lower for 35% H2O2/0.1% NaF/1% TMP; 35% H2O2/2% CaGlu, meanwhile, showed higher values, compared to the other groups (p < 0.001). Cell viability was around 9%, except for the bleaching gel containing 35% H2O2/0.1% NaF/1% TMP with 12.8% (p < 0.05). ALP was higher for groups containing TMP compared to other whitening gels (p < 0.05). The formation of mineralization nodules was greater for gels containing NaF/TMP or CaGlu (p < 0.05). The alterations of cell morphology were intense for all bleaching gels. It was concluded that the addition of NaF/TMP in-office bleaching did not interfere in bleaching efficacy, reduced enamel demineralization, H2O2 penetration and cytotoxicity. São Paulo State University (UNESP) School of Dentistry Department of Preventive and Restorative Dentistry School of Dentistry University of Ribeirão Preto - UNAERP, Ribeirão Preto São Paulo State University (UNESP) School of Dentistry Department of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry Department of Preventive and Restorative Dentistry São Paulo State University (UNESP) School of Dentistry Department of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry Department of Dental Materials and Prosthodontics FAPESP: 2018/08769–5 CAPES: 88881.068437/2014–01

Objetivo: O objetivo deste estudo foi compreender a percepção materna sobre a qualidade de vida de crianças, portadoras de cárie na primeira infância (CPI). Material e Método: O objeto de estudo foram seis mães de crianças em tratamento na Clínica de Odontologia da Universidade de Ribeirão Preto. Realizou-se entrevista semi-estruturada gravada. Os dados foram analisados segundo a técnica de Análise de Conteúdo proposta por Bardin e utilizando-se os conceitos teóricos oriundos de Bronfembrenner para avaliar aspectos da CPI que interferem no pleno desenvolvimento da pessoa desde a infância. Depreendeu-se dos dados, 03 categorias empíricas: 1ª - “o dente natural é tudo”; 2ª - “ Procura o dentista quando tem dor” e 3ª - “A mãe tem responsabilidade no aparecimento da cárie porque não limpa a boca da criança”. Resultados: Os resultados demonstraram que a influência dos aspectos sócio-econômicos e educacionais das mães tornam as crianças mais vulneráveis à CPI. As mães compreendem a importância dos dentes na saúde bucal, porém, não atribuem a presença da CPI a predispor as crianças ao desenvolvimento de problemas sistêmicos e afetar o desenvolvimento biopsicossocial. As mães apontaram falhas no atendimento das crianças e de acesso ao serviço público, assim como, o despreparo dos profissionais do serviço público e privado. Conclusão: Conclui-se que que existe necessidade de ampliação de medidas socioeducativas tanto para mães quanto crianças, assim como estratégias que visem formar e ampliar o conhecimento coletivo em saúde bucal e melhoria da Política Nacional da Saúde Bucal.Descritores: Comportamento Materno; Qualidade de Vida, Cárie Dentária; Saúde da Criança.ReferênciasCalcagnile F, Pietrunti D, Pranno N, Di Giorgio G, Ottolenghi L, Vozza I. Oral health knowledge in pre-school children: A survey among parents in central Italy. J Clin Exp Dent. 2019:11:e327-33.Chase I, Berkowitz RJ, Proskin HM, Weinstein P BR. Clinical outcomes for Early Childhood Caries (ECC): the influence of health locus of control. Eur J Paediatr Dent. 2004;2:76-80.Brasil Ministério da Saúde. Projeto SB Brasil 2003 Condições de saúde bucal da população brasileira2002-2003.http://cfo.org.br/wp-content/uploads /2009/10/04_0347_M.pdf. Published 2004. Accessed October 4, 2009.Brasil Ministério da Saúde. SB Brasil 2010: Pesquisa Nacional de Saúde Bucal. http://bvsms.saude.gov.br/bvs/publicacoes/pesquisa_nacional_saude_bucal.pdf. Published 2012. Accessed October 4, 2009.Guedes-Pinto AC. Odontopediatria. 9th ed. (Santos, ed.); 2016.Macedo LZ, Ammari MM. Cárie da primeira infância: conhecer para prevenir. Rev Rede Cuid em Saúde.2014;8:1-14.Minayo MC de S. O Desafio Do Conhecimento: Pesquisa Qualitativa Em Saúde. 1st ed. (Hucitec, ed.); 2012.Bardin L. Análise de Conteúdo. 70th ed. (France PU de, ed.). Lisboa; 1977.Alves PB. A ecologia do desenvolvimento humano: experimentos naturais e planejados. Psicol Reflexão e Crítica. 1997;10:369-73.Bronfenbrenner, U., Morris PA. The ecology of developmental processes. In: Inc. JW S, ed. Handbook of Child Psychology: Theoretical Models of Human Development. v. 1. New York; 1998:993-1028.Jiang M, Wong MCM, Chu CH, Dai L, Lo ECM. Effects of restoring SDF-treated and untreated dentine caries lesions on parental satisfaction and oral health related quality of life of preschool children. J Dent. 2019;88:103171.Vollú AL, da Costa M da EPR, Maia LC, Fonseca-Gonçalves A. Evaluation of Oral Health-Related Quality of Life to Assess Dental Treatment in Preschool Children with Early Childhood Caries: A Preliminary Study. J Clin Pediatr Dent.2018;42:37-44.Lai SHF, Wong MLW, Wong HM, McGrath CPJ, Yiu CKY. Factors influencing the oral health‐related quality of life among children with severe early childhood caries in Hong Kong.Int J Dent Hyg.2019;17:350-58.Abanto J, Carvalho TS, Mendes FM, Wanderley MT, Bönecker M, Raggio DP. Impact of oral diseases and disorders on oral health-related quality of life of preschool children. Community Dent Oral Epidemiol. 2011;39:105-14.American Academy of Pediatric Dentistry. Guidelines for Policy on Social Determinants of Children’s Oral Health and Health Disparities. Am Acad Pediatr Dent. 40:23-26.BaniHani A, Deery C, Toumba J, Munyombwe T, Duggal M. The impact of dental caries and its treatment by conventional or biological approaches on the oral health-related quality of life of children and carers. Int J Paediatr Dent. 2018;28:266-76.Knorst JK, Menegazzo GR, Emmanuelli B, Mendes FM, Ardenghi TM. Effect of neighborhood and individual social capital in early childhood on oral health-related quality of life: a 7-year cohort study. Qual Life Res.2019;28:1773-82.Marshman Z, Knapp R. Child oral health-related quality of life following treatment under dental general anaesthetic (DGA). Evid Based Dent. 2019;20:46-7.Phantumvanit P, Makino Y, Ogawa H, et al. WHO Global Consultation on Public Health Intervention against Early Childhood Caries. Community Dent Oral Epidemiol.2018;46:280-87.Zaror C, Atala-Acevedo C, Espinoza-Espinoza G, et al. Cross-cultural adaptation and psychometric evaluation of the early childhood oral health impact scale (ECOHIS) in chilean population. Health Qual Life Outcomes. 2018;16:232.Kidd E, Fejerskov O. Changing concepts in cariology: forty years on. Dent Update. 2013;40:277-86.Ferreira JMS, Silva FS, Aragão AKR, Duarte RC, Menezes VA. Conhecimento de pais sobre saúde bucal na primeira infância. Pediatr Mod.2010;46:224-30.Novaes TF, Pontes LRA, Freitas JG, et al. Responsiveness of the Early Childhood Oral Health Impact Scale (ECOHIS) is related to dental treatment complexity. Health Qual Life Outcomes.2017;15:182.Mansoori S, Mehta A, Ansari MI. Factors associated with Oral Health Related Quality of Life of children with severe -Early Childhood Caries. J Oral Biol Craniofacial Res.2019;9:222-225.Batliner T, Fehringer KA, Tiwari T, et al. Motivational interviewing with American Indian mothers to prevent early childhood caries: study design and methodology of a randomized control trial. Trials. 2014;15:125.

Made available in DSpace on 2020-12-12T01:36:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 This study evaluated the effect on enamel demineralization of 10% hydrogen peroxide (H2O2) gels containing different concentrations of sodium trimetaphosphate (TMP) and sodium fluoride (NaF) combined with the daily use of fluoridated or placebo dentifrice. Bovine enamel blocks were selected by surface hardness (n = 72) and randomly assigned to one of the following experimental treatments: 10% H2O2; 10% H2O2 + 3% TMP + 0.1% NaF; and 10% H2O2 + 0.3% TMP + 0.05% NaF, each with or without fluoridated dentifrice. H2O2-based gels were applied for 30 min d−1 followed by treatment with dentifrice (1 min). Enamels blocks were stored in artificial saliva at 37°C between sessions during the 14 days of experiment. Percentage of surface hardness loss (%SH) was calculated, and the blocks were cut into halves to analyze cross-sectional hardness (ΔKHN). Polarized light microscopy images were obtained of the longitudinal sections of the samples. Enamel treated with fluoridated dentifrice presented lower hardness loss than those treated with placebo dentifrice (%SH and ΔKHN). Use of TMP- and NaF-based gels, regardless of concentration, led to the lowest %SH values. Specimens treated with 10% H2O2 gel had the highest %SH and ΔKHN values. Gels with 10% H2O2 + 3% TMP + 0.1% NaF showed the lowest ΔKHN values. Microscopy images clearly showed that the addition of TMP and NaF to the H2O2-based gels was effective in reducing the loss of hardness, and the fluoridated dentifrice helped minimize it in all treatments. School of Dentistry São Paulo State University (Unesp) School of Dentistry São Paulo State University (Unesp)

Made available in DSpace on 2021-06-25T11:13:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T15:24:13Z : No. of bitstreams: 1 S1806-83242021000100801.pdf: 231265 bytes, checksum: 68c597a4bcc0963aef1e4bd904ce1684 (MD5) The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected according to initial surface hardness (SH), and divided into two groups: a) double combinations: S. mutans with Bifidobacterium or Lactobacillus, and b) triple combinations: S. mutans and S. sobrinus with Bifidobacterium or Lactobacillus species. The blocks were exposed to the bacterial associations for 7 days. Subsequently, quantity of EPS from biofilms and caries lesion depth were determined by means of colorimetric and cross-sectional enamel hardness (ΔKHN) analysis. The data were submitted to one-way analysis of variance, followed by the Bonferroni test (p < 0.05). S. mutans with B. animalis or B. dentium produced a higher quantity of EPS; S. mutans + B. animalis led to the highest ∆KHN. S. mutans + S. sobrinus + B. longum induced greater EPS and ∆KHN values. In conclusion, associations of B. animalis and B. longum with streptococci promoted EPS production and caries lesion progression. Universidade Estadual Paulista - Unesp School of Dentistry Department of Preventive and Restorative Dentistry Universidade Estadual Paulista - Unesp School of Dentistry Department of Preventive and Restorative Dentistry

Made available in DSpace on 2019-10-06T15:58:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01 Objective: To evaluate the effect of a fluoride toothpaste containing nano-sized sodium trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. Design: This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing four enamel bovine blocks. The cariogenic challenge was performed by 30% sucrose solution (6x/day). The toothpaste treatments (3x/day) were as follows: no F/TMP/TMPnano (Placebo), 1100 ppm F (1100F), 1100F plus 3% micrometric or nano-sized TMP (1100F/TMP; 1100F/TMPnano). Percentage of surface hardness loss (%SH), and integrated loss of subsurface hardness (ΔKHN), as well as enamel calcium (Ca), phosphorus (P), and fluoride (F) were determined. Moreover, biofilm formed on the blocks were analyzed for F, Ca, P, and insoluble extracellular polysaccharide (EPS) concentrations. Data were analyzed using one-way ANOVA, repeated measures followed by Fisher LSD test (p < 0.001). Results: 1100F/TMPnano promoted the lowest %SH and ΔKHN among all groups (p < 0.001). Regarding the F concentrations in the enamel and in the biofilm, there were no significant differences between 1100 F and 1100 F/TMPnano, but significantly increased enamel Ca concentrations (p < 0.001). 1100F/TMPnano showed lower values of EPS concentration when compared with 1100F (∼80%) (p < 0.001). Conclusion: 1100F/TMPnano promoted a greater protective effect against enamel demineralization and significantly affected the composition of biofilm formed in situ when compared to 1100F toothpaste. São Paulo State University (Unesp) School of Dentistry Araçatuba Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193 LIEC-Department of Chemistry Federal University of São Carlos (UFSCar) São Paulo State University (Unesp) School of Dentistry Araçatuba Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193

Made available in DSpace on 2019-10-06T15:20:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Objective: To evaluate the remineralizing potential of a conventional toothpaste (1,100 ppm F) supplemented with nano-sized sodium hexametaphosphate (HMPnano) in artificial caries lesions in situ. Design: This double-blinded crossed study was performed in 4 phases of 3 days each. Twelve subjects used palatal appliances containing 4 bovine enamel blocks with artificial caries lesions. Volunteers were randomly assigned into the following treatment groups: no F/HMP/HMPnano (Placebo); 1,100 ppm F (1100F); 1100F plus 0.5% micrometric HMP (1100F/HMP) and 1100F plus 0.5% nano-sized HMP (1100F/HMPnano). Volunteers were instructed to brush their natural teeth with the palatal appliances in the mouth for 1 min (3 times/day), so that blocks were treated with natural slurries of toothpastes. After each phase, surface hardness post-remineralization (SH2), integrated recovery of subsurface hardness (ΔIHR), integrated mineral recovery (ΔIMR) and enamel F concentration were determined. Data were submitted to analysis of variance and Student-Newman-Keuls' test (p < 0.001). Results: Enamel surface became 42% harder when treated with 1100F/HMPnano in comparison with 1100F (p < 0.001). Treatment with 1100F/HMP and 1100F/HMPnano promoted an increase of ∼23 and ∼87%, respectively, in ΔIHR when compared to 1100F (p < 0.001). In addition, ΔIMR for the 1100F/HMPnano was ∼75 and ∼33% higher when compared to 1100F and 1100F/HMP respectively (p < 0.001). Enamel F uptake was similar among all groups except for the placebo (p < 0.001). Conclusion: The addition of 0.5% HMPnano to a conventional fluoride toothpaste was able to promote an additional remineralizing effect of artificial caries lesions. Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of Dentistry LIEC-Department of Chemistry Federal University of São Carlos (UFSCar) Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP) School of Dentistry

Aim: To validate an in vitro caries model and to evaluate an experimental mouthwash containing Croton doctoris essential oil. Materials & methods: To validate the experimental model, we used McBain medium and polymicrobial biofilms. The EOM (essential oil mouthwash) was tested using the validated model. Microbial composition (colony-forming unit/ml), acidogenicity, enamel demineralization (percentage of surface enamel hardness loss), cytotoxicity and essential oil composition were evaluated. Results: The model was validated with 0.5% sucrose, duration of 4 days and treatments twice per day. There were statistically significant differences between the EOM, the negative control and chlorhexidine mouthwash in colony-forming unit/ml and percentage of surface enamel hardness loss. Cytotoxicity was similar to that of chlorhexidine mouthwash. A total of 66.11% of the essential oil consists of sesquiterpenes. Conclusion: The experimental mouthwash showed antimicrobial activity against polymicrobial biofilms and reduced enamel demineralization.

Made available in DSpace on 2018-12-11T17:15:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Objective This in situ study evaluated the effect of the association of low-F (4500 μg F/g) gel containing TMP and FT (1100 μg F/g) on enamel demineralization. Methods This crossover and double-blind study consisted of five phases of seven days each. Volunteers (n = 12) wore palatal appliances containing four enamel blocks. The cariogenic challenge was performed with 30% sucrose solution (six times/day). Treatments were: placebo toothpaste (PT, no fluoride/TMP); 1100 μg F/g toothpaste (FT); FT + 4500 μg F/g + 5%TMP gel (FT + TMP gel); FT + 9000 μg F/g gel (FT + 9000 gel) and FT + 12,300 μg F/g (FT + Acid gel). After topical application of treatments for one min, two blocks were removed for analysis of loosely bound fluoride (CaF2), calcium (Ca), phosphorus (P) and firmly bound fluoride (FA) formed in enamel. After the seven-day experimental periods, the percentage of surface hardness loss (%SH), integrated subsurface hardness loss (ΔKHN), CaF2, Ca, P and FA retained were determined. Moreover, the biofilms formed on the blocks were analyzed for F, Ca, P and insoluble extracellular polysaccharide (EPS) concentrations. Results FT + TMP gel promoted the lowest%SH and ΔKHN (p < 0.001). The highest concentration of CaF2 formed was observed for the FT + Acid gel (p < 0.001), followed by FT + 9000 gel > FT + TMP gel > FT > PT. CaF2 retained on the blocks was reduced across all groups (p < 0.001). Similar values were observed for the Ca/P/F and EPS in enamel and biofilm for all fluoride groups. Conclusion The association of FT + TMP gel significantly reduced enamel demineralization in situ. Clinical Significance: The association of treatments may be an alternative for patients with high caries risk. São Paulo State University (Unesp) School of Dentistry Araçatuba Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193 São Paulo State University (Unesp) School of Dentistry Araçatuba Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193

Made available in DSpace on 2018-12-11T17:23:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-11-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Objectives This study evaluated the effect of conventional (CD, 1100 ppm F) and low-fluoride (LFD, 550 ppm F) dentifrices, applied in different quantities, on enamel demineralization, and on fluoride (F) concentrations in the dental biofilm formed in situ. Methods Five combinations of dentifrices and quantities were tested: placebo (P–F-free) applied on all brush bristles; LFD applied by the transversal technique (0.3 g–T1) or on all bristles (0.6 g–T2); and CD applied in a pea-sized amount (0.15 g–T3) or by the transversal technique (0.3 g–T4), in order to produce comparable intensities (F concentration in the dentifrice × amount applied to the brush). Volunteers (n = 13, 20–36 years old) wore palatal devices containing 4 bovine enamel blocks, and performed cariogenic challenges (30% sucrose solution) 6×/day, and brushing 3×/day, following a double-blind, cross-over and randomized protocol. On the 8th day, biofilm was collected 5 and 60 min after brushing. The percentage of surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN) and biofilm F concentrations (solid and fluid phases) were determined. Data were analyzed by repeated-measures ANOVA, Student-Newman-Keuls test, and Pearson's correlation coefficient (p < 0.05). Results Significantly lower ΔKHN was observed for treatments with higher intensity (T2 and T4) in comparison with the lower intensity (T1 and T3). A strong correlation was observed between ΔKHN and F concentrations in total biofilm (r = −0.71) and biofilm fluid (r = −0.72) 5 min after brushing. Conclusions The treatment intensity has a significant influence on the development of caries lesions in situ. Clinical significance: The intensity of treatment (amount of dentifrice × concentration) during brushing seems to be a more relevant parameter of clinical efficacy than simply observing the F concentration of the product. The use of a small amount of CD significantly reduced the protective effects against enamel demineralization. São Paulo State University (Unesp) School of Dentistry Department of Pediatric Dentistry and Public Health University of São Paulo Bauru School of Dentistry Department of Biological Sciences São Paulo State University (Unesp) School of Dentistry Department of Pediatric Dentistry and Public Health FAPESP: 2014/16443-1 CNPq: 458997/2014-5

Introduction: Early Childhood Caries (ECC) is the term used to describe dental caries in children under 6 years of age. Objective: Report the aesthetic and functional rehabilitation treatment of a child with ECC. Case report: A 5-year-old male patient was attended at the to the Pediatric Dentistry Clinic of the Araçatuba Dental School (FOA/UNESP), accompanied by his mother, who reported pain and dental caries as the main complaint. On intraoral clinical examination, caries lesions were found in all upper teeth, except for tooth 55, already restored, and tooth 65, healthy. In the lower arch, caries lesions were observed in teeth 74 and 84. After clinical and radiographic diagnosis, it was decided for the manufacture of steel crown for teeth 74 and 84, extraction of teeth 51, 52, 61 and 64, installation of removable functional space maintenance device and restorations of the other affected teeth with composite resin. Conclusion: In view of the treatment performed, it was possible to recover the aesthetic, phonetic and masticatory functions, contributing to the improvement of the child’s self-esteem as well as satisfaction of family members. Oral health education carried out with the child and his parents was essential for the success of the treatment and the maintenanceof oral health. Introdução: Cárie na Primeira Infância (CPI) é o termo utilizado para descrever a cárie dentária em crianças menores de 6 anos de idade. Objetivo: Relatar o tratamento reabilitador estético e funcional de uma criança com CPI. Relato do caso: Paciente do sexo masculino, 5 anos de idade, compareceu à clínica de Odontopediatria da Faculdade de Odontologia de Araçatuba (FOA/UNESP), acompanhado pela mãe, que relatou como queixa principal dor e presença de cárie. Ao exame clínico intrabucal, constatou-se na arcada superior lesões de cárie em todos os dentes, exceto nos dentes 55, já restaurado, e dente 65, hígido. Na arcada inferior, lesões de cárie foram observadas nos dentes 74 e 84. Após diagnóstico clínico e radiográfico, optou-se por realizar a confecção de coroa de aço para os dentes 74 e 84, exodontia dos dentes 51, 52, 61 e 64, instalação de aparelho mantenedor de espaço funcional removível e restaurações dos demais dentes afetados com compósito resinoso. Conclusão: Foi possível recuperar as funções estéticas, fonéticas e mastigatórias do paciente, colaborando para a melhora da autoestima da criança, o que gerou satisfação dos familiares. A educação em saúde bucal realizada com a criança e os responsáveis foi essencial para o sucesso do tratamento e a manutenção da saúde bucal.

Made available in DSpace on 2019-10-06T16:09:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-09-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: To evaluate the effect of a fluoride toothpaste containing nano-sized sodium hexametaphosphate (HMPnano) on enamel demineralization on the biochemical composition and insoluble extracellular polysaccharide (EPS) in biofilm formed in situ. Methods: This crossover double-blind study consisted of four phases (7 days each), in which 12 volunteers wore intraoral appliances containing four enamel bovine blocks. The cariogenic challenge was performed using 30% sucrose solution (6×/day). Blocks were treated 3×/day with the following toothpastes: no F/HMP/HMPnano (Placebo), conventional fluoride toothpaste, 1100 ppm F (1100F), 1100F + 0.5% micrometric HMP (1100F/HMP), and 1100F + 0.5% nano-sized HMP (1100F/HMPnano). The percentage of surface hardness loss (%SH), integrated loss of subsurface hardness (ΔKHN), and enamel calcium (Ca), phosphorus (P), and fluoride (F) were determined. Moreover, biofilms formed on the blocks were analyzed for F, Ca, P, and insoluble extracellular polysaccharide (EPS) concentrations. Data were analyzed using one-way ANOVA, followed by Student–Newman–Keuls’ test (p < 0.001). Results: 1100F/HMPnano promoted the lowest %SH and ΔKHN among all groups (p < 0.001). The addition of HMPnano to 1100F significantly increased Ca concentrations (p < 0.001). The 1100F/HMPnano promoted lower values of EPS when compared with 1100F (~ 70%) (p < 0.001) and higher values of fluoride and calcium in the biofilms (p < 0.001). Conclusion: 1100F/HMPnano demonstrated a greater protective effect against enamel demineralization and on the composition of biofilm in situ when compared to 1100F toothpaste. Clinical relevance: This toothpaste could be a viable alternative to patients at high risk of caries. School of Dentistry Araçatuba Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP), Rua José Bonifácio 1193 LIEC-Department of Chemistry Federal University of São Carlos (UFSCar) School of Dentistry Araçatuba Department of Pediatric Dentistry and Public Health São Paulo State University (UNESP), Rua José Bonifácio 1193 FAPESP: 2016/03148-7

Made available in DSpace on 2021-06-25T10:46:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Objective: To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with sodium trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design: Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p < 0.001). Results: 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p < 0.001). F concentration was similar between the 1100 F, 1100 F-MI Paste Plus®, and 1100 F-TMP-MI Paste Plus® groups (p > 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p < 0.001). Conclusion: The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions. São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Rua José Bonifácio 1193 Araçatuba Federal University of São Carlos - UFSCar Department of Materials Engineering Northwestern University Feinberg School of Medicine Ann and Robert Lurie Children's Hospital School of Dentistry University of Ribeirão Preto - UNAERP São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Rua José Bonifácio 1193 Araçatuba

Introducción: Los traumatismos en los dientes decimales son comunes de ocurrir, siendo su primer episodio normalmente en los niños cuando están aprendiendo a caminar. Los traumas nuevos o repetidos pueden ocurrir a lo largo del crecimiento y desarrollo del niño, siendo importante su diagnóstico, seguimiento y tratamiento cuando sea necesario. Objetivo: El objetivo del presente estudio fue relatar un caso clínico de fractura corono-esmalte y subluxación en dentición decidua, bien como sus complicaciones clínicas, radiográficas y conducta clínica. Relato de caso: Paciente de sexo femenino, 2 años y 8 meses de edad, compareció a la clínica de Odontopediatría de la Facultad de Odontología de Araçatuba (FOA/UNESP), Brasil, cuya mamá relataba la aparición de una “bolita” en la región superior del diente 51 y fractura corono-esmalte. Durante la anamnesis la mamá relato que la niña se había caído hace un mes y golpeado el diente 51 presentando sangramiento, leve movilidad y fractura corono-esmalte, y que según el odontopediatría que atendió la niña en el momento del trauma le informo que se trataba de un traumatismo tipo subluxación, y que la conducta clínica, debería ser acompañamiento. Al examen clínico se observó alteración en la coloración del diente 51 y presencia de fistula en la región. Al examen radiográfico fue posible observar inicio de reabsorción radicular y lesión periapical denotando necrosis pulpar. El plan de tratamiento instaurado fue endodoncia del diente 51, seguido por acompañamiento clínico y radiográfico del mismo. Después de 8 días, la niña retorno sin la presencia de fistula, mostrando la eficacia del tratamiento instaurado. Conclusión: Se concluye, por tanto que aunque la subluxación pueda traer daños al diente deciduo, cuando es diagnosticada precozmente, la alteración es susceptible de tratamiento efectivo y satisfactorio, llevando a la preservación del diente hasta el momento de su exfoliación.Descriptores: Traumatismos de los Dientes; Diente Primario; Terapéutica.

Made available in DSpace on 2018-12-11T17:36:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objectives: To evaluate in vitro the effect of neutral pH topical gels with reduced fluoride concentration (F), supplemented or not with sodium hexametaphosphate (HMP) on the remineralization of dental enamel, using a pH-cycling model. Materials and methods Bovine enamel blocks with caries-like lesions were randomly treated with five gels (n = 24/group): without F/HMP (Placebo); 4500 ppm F (4500F), 4500F plus 9% HMP (4500F + HMP); 9000 ppm F (9000F); and 12,300 ppm F (Acid gel). After pH-cycling, the percentage of surface hardness recovery (%SHR), integrated loss of subsurface hardness (ΔKHN), and concentrations of loosely- (CaF2) and firmly-bound (FA) fluoride formed and retained in/on enamel were determined. The results were analyzed by ANOVA followed by the Student-Newman-Keuls test (p < 0.001). Results: The 4500F + HMP gel promoted the highest %SHR among all groups; the lowest ΔKHN was achieved by 4500F + HMP and Acid gel, without significant differences between these. The Acid gel group presented the highest CaF2 and FA formed and retained on/in enamel (p < 0.001). Conclusion: Based on the present results, the addition of 9% sodium hexametaphosphate to a gel with reduced fluoride concentration (4500F) was able to significantly enhance the remineralization of artificial carious lesions in vitro when compared to 4500F, reaching protective levels similar to an acidic formulation with ∼3-fold higher fluoride concentration. São Paulo State University (Unesp) School of Dentistry Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193 São Paulo State University (Unesp) School of Dentistry Department of Pediatric Dentistry and Public Health, Rua José Bonifácio 1193 FAPESP: 2014/06676-9

Made available in DSpace on 2018-12-11T17:00:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-01 Objective: The aim of this study was to evaluate the effect of fluoride dentifrices combined with sodium hexametaphosphate (HMP) on enamel demineralization in vitro. Material and methods: Enamel bovine blocks were selected by initial surface hardness (SHi) and then divided into five experimental groups (n = 12): placebo (without fluoride and without HMP); 1100 ppm F (1100F); and 1100F associated with HMP at 0.5 % (1100HMP0.5%), 1 % (1100HMP1%), and 2 % of HMP (1100HMP2%). Blocks were submitted to five pH cycles (demineralizing/remineralizing solutions) at 37 °C. After pH cycling, final surface hardness (SHf), percentage of surface hardness loss (%SH), integrated differential hardness (ΔIH), integrated loss of subsurface hardness (ΔKHN), and enamel firmly bound fluoride (F) were determined. Data were submitted to one-way ANOVA, followed by Student–Newman–Keuls test (p < 0.05). Results: Significant differences were observed among all groups regarding %SH and ΔKHN. 1100HMP1% promoted the lowest mineral loss among all groups (p < 0.001), and led to significantly lower demineralization in the deeper regions of the subsurface lesion when compared with the other HMP-containing toothpastes (p < 0.001). Significantly higher mineral loss was observed for 1100HMP2% when compared to the other fluoridated dentifrices, mainly in the outer part of the lesion (p < 0.001). Enamel F uptake was similar for 1100F and 1100HMP1% but significantly reduced for other HMP concentrations. Conclusion: The supplementation of a 1100-ppm F dentifrice with 1 % HMP promoted a higher inhibitory effect against enamel demineralization when compared to a dentifrice containing the same amount of fluoride in vitro. Clinical relevance: This dentifrice could potentially be indicated to patients at high risk of caries. Department of Pediatric Dentistry and Public Health Araçatuba Dental School University Estadual Paulista (UNESP), Rua José Bonifácio 1193 Department of Pediatric Dentistry and Public Health Araçatuba Dental School University Estadual Paulista (UNESP), Rua José Bonifácio 1193