1. Determining the Accuracy and Reliability of Indirect Calorimeters Utilizing the Methanol Combustion Technique
- Author
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Sarah T. Henes, Ronald E. Dechert, George E. Mitri, Pim Gubbels, Åsa Tornberg, Stephen Garland, Eric Ravussin, Edward L. Melanson, Jamie A. Cooper, Sepideh Kaviani, Lara R. Dugas, Marco Akkermans, Paul F.M. Schoffelen, Dale A. Schoeller, RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health, and Humane Biologie
- Subjects
DAILY ENERGY-EXPENDITURE ,0301 basic medicine ,Hot Temperature ,HIGH-FAT DIET ,Medicine (miscellaneous) ,Combustion ,chemistry.chemical_compound ,0302 clinical medicine ,ANALYSIS SYSTEMS ,Materials Testing ,energy metabolism ,Statistics ,Medicine ,Respiratory exchange ratio ,Reliability (statistics) ,indirect calorimetry ,Nutrition and Dietetics ,accuracy ,MIXING CHAMBER ,WEIGHT-GAIN ,Europe ,Regression Analysis ,Oxidation-Reduction ,Coefficient of variation ,EXERCISE ,Article ,VALIDATION ,03 medical and health sciences ,Oxygen Consumption ,Linear regression ,Calibration ,Humans ,Generalizability theory ,VALIDITY ,RESTING METABOLIC-RATE ,methanol ,reliability ,030109 nutrition & dietetics ,Pulmonary Gas Exchange ,metabolic cart ,business.industry ,Reproducibility of Results ,Calorimetry, Indirect ,Humidity ,030229 sport sciences ,GAS-EXCHANGE MEASUREMENTS ,United States ,chemistry ,Solvents ,Methanol ,business - Abstract
BackgroundSeveral indirect calorimetry (IC) instruments are commercially available, but comparative validity and reliability data are lacking. Existing data are limited by inconsistencies in protocols, subject characteristics, or single-instrument validation comparisons. The aim of this study was to compare accuracy and reliability of metabolic carts using methanol combustion as the cross-laboratory criterion.MethodsEight 20-minute methanol burn trials were completed on 12 metabolic carts. Respiratory exchange ratio (RER) and percent O-2 and CO2 recovery were calculated.ResultsFor accuracy, 1 Omnical, Cosmed Quark CPET (Cosmed), and both Parvos (Parvo Medics trueOne 2400) measured all 3 variables within 2% of the true value; both DeltaTracs and the Vmax Encore System (Vmax) showed similar accuracy in measuring 1 or 2, but not all, variables. For reliability, 8 instruments were shown to be reliable, with the 2 Omnicals ranking best (coefficient of variation [CV] ConclusionsOmnical, Parvo, Cosmed, and DeltaTrac had greater accuracy and reliability. The small number of instruments tested and expected differences in gas calibration variability limits the generalizability of conclusions. Finally, humidity and temperature could be modified in the laboratory to optimize IC conditions.
- Published
- 2018