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In multiple tissues, stem cell proliferation driving tissue regeneration is dependent on Wnt/β-catenin signaling, but the identity of the niche cells providing the Wnt proteins is not resolved. Here, we show that telocytes, unique interstitial cells that harbor long cytoplasmic extensions, are obligatory Wnt-producing niche cells in the hair follicle. Employing two independent mouse models we reveal a continuous network of interconnected telocytes that envelopes stem cells and their progeny along the path of differentiation. Telocytes compartmentalize in space and time the production of mRNA molecules that encode important signaling factors, for localized phase-dependent signaling. Ablation of either dermal telocytes, or the Wnt signals emanating from them, abrogates hair follicle regeneration, demonstrating that telocytes constitute a critical component of the hair follicle stem cell niche. We propose that telocyte production of Wnt is a universal theme in adult stem cell biology and tissue regeneration.

Northern Italy is an area particularly suitable for the cultivation of different crops. It used to be characterized by a high agrobiodiversity. However, it is estimated that over the last decades, this area experienced a genetic erosion of more than 90%, resulting in the loss of many traditional plant genetic resources (PGR). Nevertheless, recent surveys found an unexpectedly high agrobiodiversity which remains. The presented study aims to provide a complete overview of plant agrobiodiversity (in terms of crop landraces, old cultivars and neglected crops) in northern Italy using a mixed approach of literature research and ethnobotanical field surveys. In addition, this paper aims to investigate on farm and ex situ conservation activities performed in the study area. 810 entities were found, of which 26 were identified as neglected crops, 377 as landraces (plus 124 doubtful landraces) and 99 as old cultivars (plus 184 doubtful old cultivars). 248 entities were here recorded for the first time through field surveys. It clearly emerges that by using an ethnobotanical approach, several PGR can still be found in the study area especially in small farms and home gardens. We describe hotspots of crop diversity and areas in which there is still a lack of knowledge of local agrobiodiversity. Only 43% of the PGR recorded are conserved long-term in seed banks, underlying the fact that great efforts are needed to ensure the ex situ conservation of PGR in northern Italy. Careful interventions are needed to enhance the on-farm conservation status of the crop diversity reported here by enhancing its demand and its accessibility.

The sword lily Gladiolus palustris Gaudin is protected on European level and listed in Annexes II and IV of the EC Habitat Directive 92/43/EEC. It grows in nutrient-poor, calcareous meadows in central and eastern Europe. Tree encroachment in montane meadows of the European Alps as a result of recent land use changes and the abandonment of traditional farming practices threaten the survival of this species. Conservation-driven mowing is considered a feasible conservation measure for maintaining high species diversity in abandoned semi-natural grasslands. To assess the effects of ten years of biennial mowing on a grassland community in the Dolomiti Bellunesi National Park, Italy (Site of Community Importance, Natura 2000 network), ten 25 m2 plots were established whereby four plots were placed in the mowed area, four in the non-mowed area and two in a small non-mowed patch of grassland inside the mowed area. In each plot the following variables were recorded, total percentage of plant cover, percentage cover of woody species, percentage cover of herbaceous species, percentage cover and number of flowering ramets of G. palustris and a complete list of species and their percentage abundance. Mowed plots showed a higher species richness than non-mowed plots. The number of G. palustris flowering ramets and percentage cover increased manifold in mowed plots compared to non-mowed plots. The resumption of mowing for conservation purposes undertaken by the managing authority halted the process of tree encroachment and avoided a drastic change in plant composition. Periodic mowing (every second or third year) was demonstrated to be a cost-effective conservation measure in non-productive grasslands to keep grasses at bay in favour of forbs of high conversation value.

Knowledge of germination and propagation requirements are crucial for the use of plant genetic resources. Here, we compare different treatments to improve the germination of Bunias erucago (Cruciferae), a Mediterranean vegetable herb of growing commercial interest. Its cultivation is limited by the poor germination shown by the seeds which are enclosed in indehiscent silicles. To improve the germination, we investigated four treatments: (1) pre-soaking silicles in water for 48 hours; (2) scarification of silicles with sandpaper; (3) seed extraction; and (4) seed exposure to gibberellic acid (GA3). The same treatments (except the GA3 treatment) were also used to investigate seedling emergence under glasshouse conditions. In the absence of treatments and/or soaking in water, there was very low final germination (< 10%), while scarification and seed extraction increased the germination to ∼40% and > 90%, respectively. Scarification was the most effective treatment in a horticultural context, since the brittleness of seeds makes their extraction too delicate and time-consuming. The seedling emergence results confirmed those of seed germination in the laboratory, underlining the effectiveness of the treatments for plant cultivation. Seed germination performance varied among wild populations, underlining the importance of provenance when using wild plants as new crops.

Chronic liver injury, as observed in non-alcoholic steatohepatitis (NASH), progressive fibrosis, and cirrhosis, remains poorly treatable. Steatohepatitis causes hepatocyte loss in part by a direct lipotoxic insult, which is amplified by derangements in the non-parenchymal cellular (NPC) interactive network wherein hepatocytes reside, including, hepatic stellate cells, liver sinusoidal endothelial cells and liver macrophages. To create an in vitro culture model encompassing all these cells, that allows studying liver steatosis, inflammation and fibrosis caused by NASH, we here developed a fully defined hydrogel microenvironment, termed hepatocyte maturation (HepMat) gel, that supports maturation and maintenance of pluripotent stem cell (PSC) derived hepatocyte- and NPC-like cells for at least one month. The HepMat-based co-culture system modeled key molecular and functional features of TGFβ-induced liver fibrosis and fatty-acid induced inflammation and fibrosis better than monocultures of its constituent cell populations. The novel co-culture system should open new avenues for studying mechanisms underlying liver steatosis, inflammation and fibrosis as well as for assessing drugs counteracting these effects. ispartof: BIOMATERIALS vol:276 ispartof: location:Netherlands status: published

Northern Italy is an area particularly suitable for the cultivation of different crops. It used to be characterized by a high agrobiodiversity. However, it is estimated that over the last decades, this area experienced a genetic erosion of more than 95%, resulting in the loss of many traditional plant genetic resources (PGR). Nevertheless, recent surveys found an unexpectedly high agrobiodiversity which remains. The presented study aims to provide a complete overview of plant agrobiodiversity (in terms of crop landraces, old cultivars and neglected crops) in northern Italy using a mixed approach of literature research and ethnobotanical field surveys. This paper is a fundamental step towards putting in place successful on farm and ex situ conservation measures in the study area. 811 entities were found, of which 27 were identified as neglected crops, 377 as landraces (plus 124 doubtful landraces) and 99 as old cultivars (plus 184 doubtful old cultivars). 248 entities were here recorded for the first time through field surveys. It clearly emerges that by using an ethnobotanical approach, several PGR can still be found in the study area especially in small farms and home gardens. We describe hotspots of crop diversity and areas in which there is still a lack of knowledge of local agrobiodiversity. Only 43% of the PGR recorded are conserved long-term in seed banks, underlying the fact that great efforts are needed to ensure the ex situ conservation of PGR in northern Italy. Careful interventions are needed to enhance the on-farm conservation status of the crop diversity reported here by enhancing its demand and its accessibility.

Crop wild relatives are fundamental genetic resources for crop improvement. Wheat wild relatives often produce heteromorphic seeds that differ in morphological and physiological traits. Several Aegilops and Triticum species possess, within the same spikelet, a dimorphic seed pair, with one seed being larger than the other. A comprehensive analysis is needed to understand which traits are involved in seed dimorphism and if these aspects of variation in dimorphic pairs are functionally related. To this end, dispersal units of Triticum urartu and five Aegilops species were X-rayed and the different seed morphs weighed. Germination tests were carried out on seeds, both dehulled and left in their dispersal units. Controlled ageing tests were performed to detect differences in seed longevity among seed morphs, and the antioxidant profile was assessed in terms of antioxidant compounds equipment and expression of selected antioxidant genes. We used PCA to group seed morphs sharing similar patterns of germination traits, longevity estimates and antioxidant profile. Different seed morphs differed significantly in terms of mass, final germination, germination timing, longevity estimates and antioxidant profile in most of the tested species. Small seeds germinated slower, had lower germination when left in their dispersal units, a higher antioxidant potential and were longer-lived than large seeds. The antioxidant gene expression varied between morphs, with different patterns across species but not clearly reflecting the phenotypic observations. The results highlight different trait trade-offs in dimorphic seeds of Aegilops and T. urartu, affecting their germination phenology and longevity, thereby resulting in recruitment niche differentiation.

Background: tarragon ( Artemisia dracunculus L.) is a perennial herb or subshrub belonging to the Asteraceae family. It is widely used in culinary recipes from several areas of the world for its aromatic properties. This study analyses the traditional use and the cultivation of this crop in the village of Sappada/Plodn , a German linguistic island in north-eastern Italy. Here, the species, vernacularly known as “ perschtromm ”, is employed to flavour a sour soft cheese, called “ saurnshotte ”. Methods: this paper verifies the taxonomic identity of perschtromm and studies its traditional use in Sappada/Plodn. The gathered informations concerned tarragon propagation, cultivation, plant material processing and preparation of saurnshotte . The survey is based on ethnobotanical interviews integrated with literature research. Results: tarragon is still cultivated by a dozen inhabitants, but its use on domestic scale for cheese production is almost disappeared. Nevertheless, the recent revival of this tradition is bringing the production of saurnshotte from a domestic scale to a commercial one finding a place in the Sappada economy that is mostly touristic based. Discussion: tarragon is not a common ingredient in northern Italy culinary traditions and It is interesting to note that the cultivation of this crop is limited to Sappada/Plodn, not occurring in neighbouring villages. This fact supports the connection between Sappada/Plodn with Tyrolean and Carinthian regions, where Sappada inhabitants are supposed to come from. Conclusions: the survey definitely verified the taxonomic identity of perschtromm with tarragon, sustaining the identification with herbarium vouchers. The current cultivation and processing of tarragon for saurnshotte production is still a cultural heritage of the linguistic island, even if threatened by the low number of depositaries of folk knowledge and the local commercialization of fake-typical product tarragon based.

Horse liver alcohol dehydrogenase was reacted with glyoxal at different pH values ranging from 6.0 to 9.0. At pH 9.0 the enzyme undergoes a rapid activation over the first minutes of reaction, followed by a decline of activity, which reaches 10% of that of the native enzyme. Chemical analysis of the inactivated enzyme after sodium borohydride reduction shows that 11 argi-ine and 11 lysine residues per mole are modified. At pH 7.7 the enzyme activity increases during the first hour of the reaction with glyoxal and then decreases slowly. Chemical analysis shows that 4 arginine and 3 lysine residues per mole are modified in the enzyme at the maximum of activation. At pH 7.0 the enzyme undergoes a 4-fold activation. Chemical analysis shows that in this activated enzyme 3 lysine and no arginine residues per mole have been modified. Steady-state kinetic analysis suggests that the activated enzyme is not subjected to substrate inhibition and that its Michaelis constant for ethanol is three times larger than that of the native enzyme. The possible role of arginine and lysine residues in the catalytic function of liver alcohol dehydrogenase is discussed.