379 results on '"Marek L. Kowalski"'
Search Results
2. Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation
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Aleksandra Wardzyńska, Małgorzata Pawełczyk, Joanna Rywaniak, Joanna Makowska, Joanna Jamroz-Brzeska, and Marek L. Kowalski
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Asthma ,Basic immunology ,Elderly ,Innate immunity ,miRNA ,Systemic inflammation ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background The course of asthma may differ between elderly asthmatics (EA) and non-elderly asthmatics (nEA), which may be partially associated with an age-dependent aberrant immune response. The aim of the study was to determine the influence of serum miRNA expression on asthma characteristics and systemic inflammation markers in EA and nEA. Methods Control and severity of asthma, pulmonary function and FeNO were assessed in 28 EA and 31 nEA patients. The control group included 59 elderly and non-elderly healthy individuals. The expression of selected miRNAs in serum was measured with rt-PCR, and proinflammatory cytokine activity was assayed by ELISA or flow cytometry. Results No difference in serum miRNA expression was observed between the asthmatics and healthy controls. EA demonstrated lower expression of miRNA-106a and miRNA-126a than nEA (p = 0.003 and p = 0.02) and EC had lower expression of miRNA-146a, -126a, -106a and 19b than nEC (p = 0.001, p = 0.003, p = 0.005 and p
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- 2021
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3. Angioedema. Interdisciplinary diagnostic and therapeutic recommendations of the Polish Dermatological Society (PTD) and Polish Society of Allergology (PTA)
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Roman Nowicki, Elżbieta Grubska-Suchanek, Grzegorz Porębski, Marek L. Kowalski, Karina Jahnz-Różyk, Tomasz Matuszewski, Lidia Rudnicka, Marek Kulus, Wioletta Barańska-Rybak, Rafał Czajkowski, Zbigniew Doniec, Cezary Kowalewski, Beata Kręcisz, Magdalena Lange, Joanna Narbutt, Małgorzata Olszewska, Małgorzata Sokołowska-Wojdyło, Aneta Szczerkowska-Dobosz, Radosław Śpiewak, Magdalena Trzeciak, and Aleksandra Wilkowska
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angioedema ,icatibant ,ecallantide ,rituximab ,berinert ,ruconest ,firazyr ,landelumab ,Medicine ,Dermatology ,RL1-803 - Abstract
Angioedema is a non-inflammatory edema of the subcutaneous tissue and/or mucosal membranes. It commonly coexists with urticaria and is considered a deep form of urticaria. Less commonly, it occurs as an isolated disease in one of two basic forms: acquired angioedema and hereditary angioedema. Four types of acquired angioedema and seven types of hereditary angioedema were difinied. The treatment of angioedema depends on its form and etiological factors, with hereditary angioedema posing a most significant challenge.
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- 2020
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4. Associations of ficolins and mannose-binding lectin with acute myeloid leukaemia in adults
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Anna Sokołowska, Anna S. Świerzko, Gabriela Gajek, Aleksandra Gołos, Mateusz Michalski, Mateusz Nowicki, Agnieszka Szala-Poździej, Anna Wolska-Washer, Olga Brzezińska, Agnieszka Wierzbowska, Krzysztof Jamroziak, Marek L. Kowalski, Steffen Thiel, Misao Matsushita, Jens C. Jensenius, and Maciej Cedzyński
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Medicine ,Science - Abstract
Abstract We investigated clinical associations of ficolins and mannose-binding lectin (MBL) in 157 patients suffering from acute myeloid leukaemia (AML). Concentrations of ficolin-1, ficolin-2, ficolin-3 and MBL (before chemotherapy) in serum were determined as were selected polymorphisms of the corresponding genes (FCN1, FCN2, FCN3 and MBL2). The control group (C) consisted of 267 healthy unrelated individuals. Median level of ficolin-1 in patients was lower (p 7 days) fever (p = 0.026). Genotyping indicated an association of G/G homozygosity (corresponding to FCN1 gene − 542 G > A polymorphism) with malignancy [p = 0.004, OR = 2.95, 95% CI (1.41–6.16)]. Based on ROC analysis, ficolin-1, -2 and -3 may be considered candidate supplementary biomarkers of AML. Their high potential to differentiate between patients from non-malignant controls but also from persons suffering from other haematological cancers (multiple myeloma and lymphoma) was demonstrated.
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- 2020
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5. Higher efficacy of rupatadine 20 mg and 10 mg versus placebo in patients with perennial allergic rhinitis: a pooled responder analysis
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Antonio Valero, Iñaki Izquierdo, Marek L. Kowalski, Glenis K. Scadding, Jean Bousquet, and Joaquim Mullol
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PAF antagonist ,Perennial allergic rhinitis ,Responder analysis ,Rupatadine ,Second-generation H1-antihistamines ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background The clinical efficacy of rupatadine in terms of responders has not been previously explored in perennial allergic rhinitis (PAR). Methods This pooled analysis included data from 6 randomised, double-blind, placebo-controlled trials conducted in PAR patients treated with rupatadine 10 mg or 20 mg, or placebo. Participants were aged ≥ 18 years, with diagnosis of PAR and a Total 4 Nasal Symptom Score (T4NSS) ≥ 5. We evaluated the T4NSS and Total 5 Symptom Score (T5SS) for 28 days of treatment, the responder proportion (50% and 75% response), and the time to response. Results Efficacy data from 1486 patients were analysed: 585 received placebo, 682 rupatadine 10 mg, and 219 rupatadine 20 mg. Compared with placebo, rupatadine promoted greater symptom improvements and higher responder proportions (50% and 75% response) for T4NSS and T5SS over 28 days. Symptom improvements and responder proportions were higher in the rupatadine 20 mg group vs the 10 mg group. The time to response was shorter in the rupatadine 20 mg group vs the 10 mg group for T4NSS (16 and 9 days for the 50% and 75% responses, respectively) and for T5SS (13 and 8 days for the 50% and 75% responses, respectively). Conclusions Rupatadine was efficacious in reducing allergic rhinitis symptoms, showing high responder proportions. The faster and stronger effect of rupatadine 20 mg may suggest its use in patients with severe PAR or not responding to the standard dose.
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- 2020
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6. The profile of respiratory pathogens in induced sputum of elderly and non-elderly asthmatics
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Aleksandra Wardzyńska, Małgorzata Pawełczyk, Anna Głobińska, Joanna S. Makowska, and Marek L. Kowalski
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asthma ,elderly ,pathogens ,induced sputum ,Medicine - Published
- 2020
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7. Regulated on Activation, Normal T cell Expressed and Secreted (RANTES) drives the resolution of allergic asthma
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Nina Li, Hoomann Mirzakhani, Alexander Kiefer, Julia Koelle, Tytti Vuorinen, Manfred Rauh, Zuqin Yang, Susanne Krammer, Paraskevi Xepapadaki, Anna Lewandowska-Polak, Heikki Lukkarinen, Nan Zhang, Barbara Stanic, Theodor Zimmermann, Marek L. Kowalski, Tuomas Jartti, Claus Bachert, Mübeccel Akdis, Nikolaos G. Papadopoulos, Benjamin A. Raby, Scott T. Weiss, and Susetta Finotto
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Biological sciences ,Immunology ,Respiratory medicine ,Science - Abstract
Summary: RANTES is implicated in allergic asthma and in T cell-dependent clearance of infection. RANTES receptor family comprises CCR1, CCR3, and CCR5, which are G-protein-coupled receptors consisting of seven transmembrane helices. Infections with respiratory viruses like Rhinovirus cause induction of RANTES production by epithelial cells. Here, we studied the role of RANTES in the peripheral blood mononuclear cells in cohorts of children with and without asthma and validated and extended this study to the airways of adults with and without asthma. We further translated these studies to a murine model of asthma induced by house dust mite allergen in wild-type RANTES and CCR5-deficient mice. Here we show an unpredicted therapeutic role of RANTES in the resolution of allergen-induced asthma by orchestrating the transition of effector GATA-3+CD4+ T cells into immune-regulatory-type T cells and inflammatory eosinophils into resident eosinophils as well as increased IL-10 production in the lung.
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- 2021
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8. Erratum to 'IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper' [World Allergy Organ J 13/2 (2020) 100080]
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Ignacio J. Ansotegui, Giovanni Melioli, Giorgio Walter Canonica, Luis Caraballo, Elisa Villa, Motohiro Ebisawa, Giovanni Passalacqua, Eleonora Savi, Didier Ebo, R.Maximiliano Gómez, Olga Luengo Sánchez, John J. Oppenheimer, Erika Jensen-Jarolim, David A. Fischer, Tari Haahtela, Martti Antila, Jean J. Bousquet, Victoria Cardona, Wen Chin Chiang, Pascal M. Demoly, Lawrence M. DuBuske, Marta Ferrer Puga, Roy Gerth van Wijk, Sandra Nora González Díaz, Alexei Gonzalez-Estrada, Edgardo Jares, Ayse Füsun Kalpaklioğlu, Luciana Kase Tanno, Marek L. Kowalski, Dennis K. Ledford, Olga Patricia Monge Ortega, Mário Morais-Almeida, Oliver Pfaar, Lars K. Poulsen, Ruby Pawankar, Harald E. Renz, Antonino G. Romano, Nelson A. Rosário Filho, Lanny Rosenwasser, Mario A. Sánchez Borges, Enrico Scala, Gian-Enrico Senna, Juan Carlos Sisul, Mimi L.K. Tang, Bernard Yu-Hor Thong, Rudolf Valenta, Robert A. Wood, and Torsten Zuberbier
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Immunologic diseases. Allergy ,RC581-607 - Published
- 2021
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9. Estimating the Risk of Severe Peanut Allergy Using Clinical Background and IgE Sensitization Profiles
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Mareen R. Datema, Sarah A. Lyons, Montserrat Fernández-Rivas, Barbara Ballmer-Weber, André C. Knulst, Riccardo Asero, Laura Barreales, Simona Belohlavkova, Frédéric de Blay, Michael Clausen, Ruta Dubakiene, Cristina Fernández-Perez, Philipp Fritsche, David Gislason, Karin Hoffmann-Sommergruber, Monika Jedrzejczak-Czechowicz, Laurian Jongejan, Marek L. Kowalski, Tanya Z. Kralimarkova, Jonas Lidholm, Nikolaos G. Papadopoulos, Todor A. Popov, Nayade del Prado, Ashok Purohit, Isabel Reig, Suranjith L. Seneviratne, Athanassios Sinaniotis, Emilia Vassilopoulou, Serge A. Versteeg, Stefan Vieths, Paco M. J. Welsing, E. N. Clare Mills, Thuy-My Le, Aeilko H. Zwinderman, and Ronald van Ree
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EuroPrevall ,iFAAM ,peanut allergy ,severity ,prediction ,clinical background ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background: It is not well-understood why symptom severity varies between patients with peanut allergy (PA).Objective: To gain insight into the clinical profile of subjects with mild-to-moderate and severe PA, and investigate individual and collective predictive accuracy of clinical background and IgE to peanut extract and components for PA severity.Methods: Data on demographics, patient history and sensitization at extract and component level of 393 patients with probable PA (symptoms ≤ 2 h + IgE sensitization) from 12 EuroPrevall centers were analyzed. Univariable and penalized multivariable regression analyses were used to evaluate risk factors and biomarkers for severity.Results: Female sex, age at onset of PA, symptoms elicited by skin contact with peanut, family atopy, atopic dermatitis, house dust mite and latex allergy were independently associated with severe PA; birch pollen allergy with mild-to-moderate PA. The cross-validated AUC of all clinical background determinants combined (0.74) was significantly larger than the AUC of tests for sensitization to extract (0.63) or peanut components (0.54–0.64). Although larger skin prick test wheal size, and higher IgE to peanut extract, Ara h 1 and Ara h 2/6, were associated with severe PA, and higher IgE to Ara h 8 with mild-to-moderate PA, addition of these measurements of sensitization to the clinical background model did not significantly improve the AUC.Conclusions: Models combining clinical characteristics and IgE sensitization patterns can help establish the risk of severe reactions for peanut allergic patients, but clinical background determinants are most valuable for predicting severity of probable PA in an individual patient.
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- 2021
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10. Next-generation ARIA care pathways for rhinitis and asthma: a model for multimorbid chronic diseases
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J. Jean Bousquet, Holger J. Schünemann, Alkis Togias, Marina Erhola, Peter W. Hellings, Torsten Zuberbier, Ioana Agache, Ignacio J. Ansotegui, Josep M. Anto, Claus Bachert, Sven Becker, Martin Bedolla-Barajas, Michael Bewick, Sinthia Bosnic-Anticevich, Isabelle Bosse, Louis P. Boulet, Jean Marc Bourrez, Guy Brusselle, Niels Chavannes, Elisio Costa, Alvaro A. Cruz, Wienczyslawa Czarlewski, Wytske J. Fokkens, Joao A. Fonseca, Mina Gaga, Tari Haahtela, Maddalena Illario, Ludger Klimek, Piotr Kuna, Violeta Kvedariene, L. T. T. Le, Desiree Larenas-Linnemann, Daniel Laune, Olga M. Lourenço, Enrica Menditto, Joaquin Mullol, Yashitaka Okamoto, Nikos Papadopoulos, Nhân Pham-Thi, Robert Picard, Hilary Pinnock, Nicolas Roche, Regina E. Roller-Wirnsberger, Christine Rolland, Boleslaw Samolinski, Aziz Sheikh, Sanna Toppila-Salmi, Ioanna Tsiligianni, Arunas Valiulis, Erkka Valovirta, Tuula Vasankari, Maria-Teresa Ventura, Samantha Walker, Sian Williams, Cezmi A. Akdis, Isabella Annesi-Maesano, Sylvie Arnavielhe, Xavier Basagana, Eric Bateman, Anna Bedbrook, K. S. Bennoor, Samuel Benveniste, Karl C. Bergmann, Slawomir Bialek, Nils Billo, Carsten Bindslev-Jensen, Leif Bjermer, Hubert Blain, Mateo Bonini, Philippe Bonniaud, Jacques Bouchard, Vitalis Briedis, Christofer E. Brightling, Jan Brozek, Roland Buhl, Roland Buonaiuto, Giorgo W. Canonica, Victoria Cardona, Ana M. Carriazo, Warner Carr, Christine Cartier, Thomas Casale, Lorenzo Cecchi, Alfonso M. Cepeda Sarabia, Eka Chkhartishvili, Derek K. Chu, Cemal Cingi, Elaine Colgan, Jaime Correia de Sousa, Anne Lise Courbis, Adnan Custovic, Biljana Cvetkosvki, Gennaro D’Amato, Jane da Silva, Carina Dantas, Dejand Dokic, Yves Dauvilliers, Antoni Dedeu, Giulia De Feo, Philippe Devillier, Stefania Di Capua, Marc Dykewickz, Ruta Dubakiene, Motohiro Ebisawa, Yaya El-Gamal, Esben Eller, Regina Emuzyte, John Farrell, Antjie Fink-Wagner, Alessandro Fiocchi, Jean F. Fontaine, Bilun Gemicioğlu, Peter Schmid-Grendelmeir, Amiran Gamkrelidze, Judith Garcia-Aymerich, Maximiliano Gomez, Sandra González Diaz, Maia Gotua, Nick A. Guldemond, Maria-Antonieta Guzmán, Jawad Hajjam, John O’B Hourihane, Marc Humbert, Guido Iaccarino, Despo Ierodiakonou, Juan C. Ivancevich, Guy Joos, Ki-Suck Jung, Marek Jutel, Igor Kaidashev, Omer Kalayci, Przemyslaw Kardas, Thomas Keil, Mussa Khaitov, Nikolai Khaltaev, Jorg Kleine-Tebbe, Marek L. Kowalski, Vicky Kritikos, Inger Kull, Lisa Leonardini, Philip Lieberman, Brian Lipworth, Karin C. Lodrup Carlsen, Claudia C. Loureiro, Renaud Louis, Alpana Mair, Gert Marien, Bassam Mahboub, Joao Malva, Patrick Manning, Esteban De Manuel Keenoy, Gailen D. Marshall, Mohamed R. Masjedi, Jorge F. Maspero, Eve Mathieu-Dupas, Poalo M. Matricardi, Eric Melén, Elisabete Melo-Gomes, Eli O. Meltzer, Jacques Mercier, Neven Miculinic, Florin Mihaltan, Branislava Milenkovic, Giuliana Moda, Maria-Dolores Mogica-Martinez, Yousser Mohammad, Steve Montefort, Ricardo Monti, Mario Morais-Almeida, Ralf Mösges, Lars Münter, Antonella Muraro, Ruth Murray, Robert Naclerio, Luigi Napoli, Leila Namazova-Baranova, Hugo Neffen, Kristoff Nekam, Angelo Neou, Enrico Novellino, Dieudonné Nyembue, Robin O’Hehir, Ken Ohta, Kimi Okubo, Gabrielle Onorato, Solange Ouedraogo, Isabella Pali-Schöll, Susanna Palkonen, Peter Panzner, Hae-Sim Park, Jean-Louis Pépin, Ana-Maria Pereira, Oliver Pfaar, Ema Paulino, Jim Phillips, Davor Plavec, Ted A. Popov, Fabienne Portejoie, David Price, Emmanuel P. Prokopakis, Benoit Pugin, Filip Raciborski, Rojin Rajabian-Söderlund, Sietze Reitsma, Xavier Rodo, Antonino Romano, Nelson Rosario, Menahenm Rottem, Dermot Ryan, Johanna Salimäki, Mario M. Sanchez-Borges, Juan-Carlos Sisul, Dirceu Solé, David Somekh, Talant Sooronbaev, Milan Sova, Otto Spranger, Cristina Stellato, Rafael Stelmach, Charlotte Suppli Ulrik, Michel Thibaudon, Teresa To, Ana Todo-Bom, Peter V. Tomazic, Antonio A. Valero, Rudolph Valenta, Marylin Valentin-Rostan, Rianne van der Kleij, Olivier Vandenplas, Giorgio Vezzani, Frédéric Viart, Giovanni Viegi, Dana Wallace, Martin Wagenmann, De Y. Wang, Susan Waserman, Magnus Wickman, Dennis M. Williams, Gary Wong, Piotr Wroczynski, Panayiotis K. Yiallouros, Arzu Yorgancioglu, Osman M. Yusuf, Heahter J. Zar, Stéphane Zeng, Mario Zernotti, Luo Zhang, Nan S. Zhong, Mihaela Zidarn, the ARIA Study Group, and the MASK Study Group
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Health care transformation ,Care pathways ,Rhinitis ,ARIA ,MASK ,POLLAR ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background In all societies, the burden and cost of allergic and chronic respiratory diseases are increasing rapidly. Most economies are struggling to deliver modern health care effectively. There is a need to support the transformation of the health care system into integrated care with organizational health literacy. Main body As an example for chronic disease care, MASK (Mobile Airways Sentinel NetworK), a new project of the ARIA (Allergic Rhinitis and its Impact on Asthma) initiative, and POLLAR (Impact of Air POLLution on Asthma and Rhinitis, EIT Health), in collaboration with professional and patient organizations in the field of allergy and airway diseases, are proposing real-life ICPs centred around the patient with rhinitis, and using mHealth to monitor environmental exposure. Three aspects of care pathways are being developed: (i) Patient participation, health literacy and self-care through technology-assisted “patient activation”, (ii) Implementation of care pathways by pharmacists and (iii) Next-generation guidelines assessing the recommendations of GRADE guidelines in rhinitis and asthma using real-world evidence (RWE) obtained through mobile technology. The EU and global political agendas are of great importance in supporting the digital transformation of health and care, and MASK has been recognized by DG Santé as a Good Practice in the field of digitally-enabled, integrated, person-centred care. Conclusion In 20 years, ARIA has considerably evolved from the first multimorbidity guideline in respiratory diseases to the digital transformation of health and care with a strong political involvement.
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- 2019
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11. IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
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Ignacio J. Ansotegui, MD, PhD, Giovanni Melioli, MD, Giorgio Walter Canonica, MD, Luis Caraballo, MD, PhD, Elisa Villa, MD, PhD, Motohiro Ebisawa, MD, PhD, Giovanni Passalacqua, MD, Eleonora Savi, MD, Didier Ebo, MD, PhD, R. Maximiliano Gómez, MD, PhD, Olga Luengo Sánchez, MD, PhD, John J. Oppenheimer, MD, Erika Jensen-Jarolim, MD, David A. Fischer, MD, Tari Haahtela, MD, Martti Antila, MD, Jean J. Bousquet, MD, PhD, Victoria Cardona, MD, PhD, Wen Chin Chiang, MBBS, Pascal M. Demoly, MD, PhD, Lawrence M. DuBuske, MD, Marta Ferrer Puga, MD, Roy Gerth van Wijk, MD, PhD, Sandra Nora González Díaz, MD, PhD, Alexei Gonzalez-Estrada, MD, Edgardo Jares, MD, Ayse Füsun Kalpaklioğlu, MD, Luciana Kase Tanno, MD, PhD, Marek L. Kowalski, MD, PhD, Dennis K. Ledford, MD, Olga Patricia Monge Ortega, MD, Mário Morais Almeida, MD, Oliver Pfaar, MD, PhD, Lars K. Poulsen, PhD, Ruby Pawankar, MD, PhD, Harald E. Renz, MD, PhD, Antonino G. Romano, MD, PhD, Nelson A. Rosário Filho, MD, PhD, Lanny Rosenwasser, MD, Mario A. Sánchez Borges, MD, Enrico Scala, MD, Gian-Enrico Senna, MD, Juan Carlos Sisul, MD, Mimi L.K. Tang, MBBS, PHD, Bernard Yu-Hor Thong, MD, Rudolf Valenta, MD, Robert A. Wood, MD, and Torsten Zuberbier, MD, PhD
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Currently, testing for immunoglobulin E (IgE) sensitization is the cornerstone of diagnostic evaluation in suspected allergic conditions. This review provides a thorough and updated critical appraisal of the most frequently used diagnostic tests, both in vivo and in vitro. It discusses skin tests, challenges, and serological and cellular in vitro tests, and provides an overview of indications, advantages and disadvantages of each in conditions such as respiratory, food, venom, drug, and occupational allergy. Skin prick testing remains the first line approach in most instances; the added value of serum specific IgE to whole allergen extracts or components, as well as the role of basophil activation tests, is evaluated. Unproven, non-validated, diagnostic tests are also discussed. Throughout the review, the reader must bear in mind the relevance of differentiating between sensitization and allergy; the latter entails not only allergic sensitization, but also clinically relevant symptoms triggered by the culprit allergen. Keywords: IgE, Allergy, In vitro tests, Skin tests, Diagnostic strategies
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- 2020
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12. Associations of Ficolins With Hematological Malignancies in Patients Receiving High-Dose Chemotherapy and Autologous Hematopoietic Stem Cell Transplantations
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Anna S. Świerzko, Mateusz Michalski, Anna Sokołowska, Mateusz Nowicki, Agnieszka Szala-Poździej, Łukasz Eppa, Iwona Mitrus, Anna Szmigielska-Kapłon, Małgorzata Sobczyk-Kruszelnicka, Katarzyna Michalak, Aleksandra Gołos, Agnieszka Wierzbowska, Sebastian Giebel, Krzysztof Jamroziak, Marek L. Kowalski, Olga Brzezińska, Steffen Thiel, Misao Matsushita, Jens C. Jensenius, Gabriela Gajek, and Maciej Cedzyński
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complement ,FCN1 ,FCN2 ,FCN3 ,ficolin ,hematopoietic stem cell transplantation (HSCT) ,Immunologic diseases. Allergy ,RC581-607 - Abstract
A prospective study of 312 patients [194 with multiple myeloma (MM) and 118 with lymphomas (LYMPH)] receiving high-dose chemotherapy and autologous hematopoietic stem cell transplantation (auto-HSCT) was conducted. Ficolins are innate immune defense factors, able to distinguish between “self” “abnormal self,” and “non-self” and contribute to the elimination of the last two by direct opsonization and/or initiation of complement activation via the lectin pathway. Concentrations of ficolin-1, ficolin-2, and ficolin-3 in serially taken serum samples were determined as were the polymorphisms of the corresponding (FCN1, FCN2, and FCN3) genes. Serum samples were collected before conditioning chemotherapy, before HSCT, and once weekly post-HSCT (four to five samples in total); some patients were also sampled at 1 and/or 3 months post-transplantation. The control group (C) consisted of 267 healthy unrelated individuals. Median ficolin-1 and ficolin-2 (but not ficolin-3) levels in MM patients' sera taken before chemotherapy were lower (and correspondingly frequencies of the lowest concentrations were higher) compared with controls. That appeared to be associated with the malignant disease itself rather than with post-HSCT complications (febrile neutropenia, infections accompanied, or not with bacteremia). Higher frequencies of the FCN1 genotype G/A-C/C-G/G (corresponding to polymorphisms at positions −542, −144, and +6658, respectively) and FCN2 gene heterozygosity for the −857 C>A polymorphism were found among patients diagnosed with MM compared with the C group. Furthermore, FCN2 G/G homozygosity (−557 A>G) was found more frequently and heterozygosity G/T at +6424 less frequently among LYMPH patients than among the healthy subjects. Heterozygosity for +1637delC mutation of the FCN3 gene was more common among patients diagnosed with lymphomas who experienced hospital infections. Although no evidence for an association of low ficolin-1 or ficolin-2 with infections during neutropenia following chemotherapy before HSCT was found, we observed a possible protective effect of ficolins during follow-up.
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- 2020
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13. Risk and safety requirements for diagnostic and therapeutic procedures in allergology: World Allergy Organization Statement
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Marek L. Kowalski, Ignacio Ansotegui, Werner Aberer, Mona Al-Ahmad, Mubeccel Akdis, Barbara K. Ballmer-Weber, Kirsten Beyer, Miguel Blanca, Simon Brown, Chaweewan Bunnag, Arnaldo Capriles Hulett, Mariana Castells, Hiok Hee Chng, Frederic De Blay, Motohiro Ebisawa, Stanley Fineman, David B. K. Golden, Tari Haahtela, Michael Kaliner, Connie Katelaris, Bee Wah Lee, Joanna Makowska, Ulrich Muller, Joaquim Mullol, John Oppenheimer, Hae-Sim Park, James Parkerson, Giovanni Passalacqua, Ruby Pawankar, Harald Renz, Franziska Rueff, Mario Sanchez-Borges, Joaquin Sastre, Glenis Scadding, Scott Sicherer, Pongsakorn Tantilipikorn, James Tracy, Vera van Kempen, Barbara Bohle, G Walter Canonica, Luis Caraballo, Maximiliano Gomez, Komei Ito, Erika Jensen-Jarolim, Mark Larche, Giovanni Melioli, Lars K. Poulsen, Rudolf Valenta, and Torsten Zuberbier
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract One of the major concerns in the practice of allergy is related to the safety of procedures for the diagnosis and treatment of allergic disease. Management (diagnosis and treatment) of hypersensitivity disorders involves often intentional exposure to potentially allergenic substances (during skin testing), deliberate induction in the office of allergic symptoms to offending compounds (provocation tests) or intentional application of potentially dangerous substances (allergy vaccine) to sensitized patients. These situations may be associated with a significant risk of unwanted, excessive or even dangerous reactions, which in many instances cannot be completely avoided. However, adverse reactions can be minimized or even avoided if a physician is fully aware of potential risk and is prepared to appropriately handle the situation. Information on the risk of diagnostic and therapeutic procedures in allergic diseases has been accumulated in the medical literature for decades; however, except for allergen specific immunotherapy, it has never been presented in a systematic fashion. Up to now no single document addressed the risk of the most commonly used medical procedures in the allergy office nor attempted to present general requirements necessary to assure the safety of these procedures. Following review of available literature a group of allergy experts within the World Allergy Organization (WAO), representing various continents and areas of allergy expertise, presents this report on risk associated with diagnostic and therapeutic procedures in allergology and proposes a consensus on safety requirements for performing procedures in allergy offices. Optimal safety measures including appropriate location, type and required time of supervision, availability of safety equipment, access to specialized emergency services, etc. for various procedures have been recommended. This document should be useful for allergists with already established practices and experience as well as to other specialists taking care of patients with allergies.
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- 2016
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14. Decrease of IL-5 Production by Naive T Cells Cocultured with IL-18-Producing BCG-Pulsed Dendritic Cells from Patients Allergic to House Dust Mite
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Magdalena Kowalewicz-Kulbat, Piotr Szpakowski, Krzysztof T. Krawczyk, Marek L. Kowalski, Slawomir Kosinski, Franck Biet, Wieslawa Rudnicka, and Camille Locht
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BCG ,rBCG-hIL-18 ,dendritic cells ,Th1/Th2 cells ,asthma ,Der p 1 ,Medicine - Abstract
The only currently available anti-tuberculosis vaccine, Bacillus Calmette–Guérin (BCG), has been reported to also protect against unrelated diseases, including inflammatory diseases such as allergic asthma. Recombinant BCG strains that produce IL-18 have been shown to enhance Th1 responses over non-recombinant BCG and to reduce IL-5 production and bronchoalveolar eosinophilia in mice. However, their ability to decrease the immune polarization of human Th2 cells is not known. Here, we show that BCG and recombinant BCG producing human IL-18 (rBCG-hIL-18) induced the maturation of Der p 1-stimulated monocyte-derived dendritic cells (MD-DCs) from healthy controls and from patients allergic to house dust mites. After incubation with mycobacteria and Der p 1, MD-DCs produced significantly more IL-23 and IP-10 but had no effect on IL-12p70 or IL-10 production compared to Der p 1-pulsed MD-DCs in the absence of mycobacteria. In the presence of Der p 1, BCG- and rBCG-hIL-18-pulsed MD-DCs cocultured with naive, but not with memory T cells from allergic patients, resulted in a decrease in IL-5 production compared to non-pulsed MD-DCs cultured in the presence of Der p 1. BCG, and especially rBCG-hIL-18, may thus be potential therapeutic tools to reduce exacerbated Th2 responses in patients with allergic asthma.
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- 2021
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15. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns
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Joanna Matysiak, Eliza Matuszewska, Marek L. Kowalski, Sławomir W. Kosiński, Ewa Smorawska-Sabanty, and Jan Matysiak
- Subjects
Hymenoptera venom allergy ,MALDI-TOF MS ,protein–peptide profiling ,proteomics ,venom immunotherapy ,Medicine - Abstract
Venom immunotherapy (VIT) is administered to allergic patients to reduce the risk of dangerous systemic reactions following an insect sting. To better understand the mechanism of this treatment and its impact on the human organism, we analysed serum proteomic patterns obtained at five time-points from Hymenoptera-venom-allergic patients undergoing VIT. For statistical analyses, patients were additionally divided into two groups (high responders and low responders) according to serum sIgG4 levels. VIT was found to be associated with changes in seven proteins: the fibrinogen alpha chain, complement C4-A, complement C3, filamin-B, kininogen-1, myosin-9 and inter-alpha-trypsin inhibitor heavy chain H1. The number of discriminative m/z (mass-to-charge ratio) features increased up to the 90th day of VIT, which may be associated with the development of immunity after the administration of increased venom doses. It may also suggest that during VIT, there may occur processes involved not only in protein synthesis but also in protein degradation (caused by proteolytic venom components). The results are consistent with measured serum sIgG4 levels, which increased from 2.04 mgA/I at baseline to 7.25 mgA/I at 90 days. Moreover, the major proteomic changes were detected separately in the high responder group. This may suggest that changes in protein–peptide profiles reflect the actual response to VIT.
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- 2021
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16. Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01—The Significance of the New Molecular Background
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Magdalena Stasiak, Bogusław Tymoniuk, Renata Michalak, Bartłomiej Stasiak, Marek L. Kowalski, and Andrzej Lewiński
- Subjects
hla-b*35 ,hla-b*18:01 ,hla-drb1*01 ,hla-c*04:01 ,subacute thyroiditis ,Medicine - Abstract
Subacute thyroiditis (SAT) is a thyroid inflammatory disease whose pathogenesis is still not completely defined. Previous viral infection is considered to be a triggering factor in genetically predisposed individuals. In about 70% of patients, susceptibility to SAT is associated with the HLA-B*35 allele. The correlation between SAT and other human leukocyte antigens (HLA) has not yet been unequivocally demonstrated and the genetic background is still unknown in about 30% of patients. The purpose of our study was to perform HLA genotyping using a next-generation sequencing method, to find out whether alleles other than HLA-B*35 are correlated with SAT morbidity. HLA-A, -B, -C, -DQB1, -DRB1 were genotyped using a next-generation sequencing method in 1083 subjects, including 60 SAT patients and 1023 healthy controls. Among 60 patients diagnosed with SAT, 81.7% of subjects were identified as having allele HLA-B*35, 23.3% had HLA-B*18:01, 28.3% had HLA-DRB1*01 and 75.5% had HLA-C*04:01. These alleles occurred in the control group at frequencies of 10.2%, 7.2%, 12.9% and 12.5%, respectively. The differences were statistically significant, with p < 0.05. In addition to its previously described relationship with HLA-B*35, genetic susceptibility to SAT was associated with the presence of HLA-B*18:01, DRB1*01 and C*04:01. The alleles HLA-B*18:01 and DRB1*01 were independent SAT risk factors. The assessment of these four alleles allows the confirmation of genetic predisposition in almost all patients with SAT.
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- 2020
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17. Evolution of Airway Inflammation in Preschoolers with Asthma—Results of a Two-Year Longitudinal Study
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Paraskevi Xepapadaki, Paraskevi Korovessi, Claus Bachert, Susetta Finotto, Tuomas Jartti, John Lakoumentas, Marek L. Kowalski, Anna Lewandowska-Polak, Heikki Lukkarinen, Nan Zhang, Theodor Zimmermann, and Nikolaos G. Papadopoulos
- Subjects
asthma ,predicta ,preschool ,feno ,spirometry ,Medicine - Abstract
Fractional exhaled nitric oxide (FeNO) is a non-invasive marker for eosinophilic airway inflammation and has been used for monitoring asthma. Here, we assess the characteristics of FeNO from preschool to school age, in parallel with asthma activity. A total of 167 asthmatic children and 66 healthy, age-matched controls were included in the 2-year prospective PreDicta study evaluating wheeze/asthma persistence in preschool-aged children. Information on asthma/rhinitis activity, infections and atopy was recorded at baseline. Follow-up visits were performed at 6-month intervals, as well as upon exacerbation/cold and 4−6 weeks later in the asthmatic group. We obtained 539 FeNO measurements from asthmatics and 42 from controls. At baseline, FeNO values did not differ between the two groups (median: 3.0 ppb vs. 2.0 ppb, respectively). FeNO values at 6, 12, 18 and 24 months (4.0, CI: 0.0−8.6; 6.0, CI: 2.8−12.0; 8.0, CI: 4.0−14.0; 8.5, CI: 4.4−14.5 ppb, respectively) increased with age (correlation p ≤ 0.001) and atopy (p = 0.03). FeNO was non-significantly increased from baseline to the symptomatic visit, while it decreased after convalescence (p = 0.007). Markers of disease activity, such as wheezing episodes and days with asthma were associated with increased FeNO values during the study (p < 0.05 for all). Age, atopy and disease activity were found to be important FeNO determinants in preschool children. Longitudinal and individualized FeNO assessment may be valuable in monitoring asthmatic children with recurrent wheezing or mild asthma.
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- 2020
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18. The Role of Complement Activating Collectins and Associated Serine Proteases in Patients With Hematological Malignancies, Receiving High-Dose Chemotherapy, and Autologous Hematopoietic Stem Cell Transplantations (Auto-HSCT)
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Anna S. Świerzko, Mateusz Michalski, Anna Sokołowska, Mateusz Nowicki, Łukasz Eppa, Agnieszka Szala-Poździej, Iwona Mitrus, Anna Szmigielska-Kapłon, Małgorzata Sobczyk-Kruszelnicka, Katarzyna Michalak, Aleksandra Gołos, Agnieszka Wierzbowska, Sebastian Giebel, Krzysztof Jamroziak, Marek L. Kowalski, Olga Brzezińska, Steffen Thiel, Jens C. Jensenius, Katarzyna Kasperkiewicz, and Maciej Cedzyński
- Subjects
CL-LK ,collectin ,complement ,hematopoietic stem cell transplantation (HSCT) ,mannose-binding lectin (MBL) ,MASP ,Immunologic diseases. Allergy ,RC581-607 - Abstract
We conducted a prospective study of 312 patients (194 with multiple myeloma, 118 with lymphomas) receiving high-dose conditioning chemotherapy and autologous hematopoietic stem cell transplantation (auto-HSCT). Polymorphisms of MBL2 and MASP2 genes were investigated and serial measurements of serum concentrations of mannose-binding lectin (MBL), CL-LK collectin and MASP-2 as well as activities of MBL-MASP-1 and MBL-MASP-2 complex were made. Serum samples were taken before conditioning chemotherapy, before HSCT and once weekly after (totally 4-5 samples); in minority of subjects also 1 and/or 3 months post transplantation. The results were compared with data from 267 healthy controls and analyzed in relation to clinical data to explore possible associations with cancer and with chemotherapy-induced medical complications. We found a higher frequency of MBL deficiency-associated genotypes (LXA/O or O/O) among multiple myeloma patients compared with controls. It was however not associated with hospital infections or post-HSCT recovery of leukocytes, but seemed to be associated with the most severe infections during follow-up. Paradoxically, high MBL serum levels were a risk factor for prolonged fever and some infections. The first possible association of MBL2 gene 3′-untranslated region polymorphism with cancer (lymphoma) in Caucasians was noted. Heterozygosity for MASP2 gene +359 A>G mutation was relatively frequent in lymphoma patients who experienced bacteremia during hospital stay. The median concentration of CL-LK was higher in myeloma patients compared with healthy subjects. Chemotherapy induced marked increases in serum MBL and MASP-2 concentrations, prolonged for several weeks and relatively slighter decline in CL-LK level within 1 week. Conflicting findings on the influence of MBL on infections following chemotherapy of myeloma and lymphoma have been reported. Here we found no evidence for an association between MBL deficiency and infection during the short period of neutropenia following conditioning treatment before HSCT. However, we noted a possible protective effect of MBL during follow-up, and suspected that to be fully effective when able to act in combination with phagocytic cells after their recovery.
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- 2018
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19. Changes in the concentration of pollen over an 11-year period in a Polish urban environment
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Barbara Majkowska-Wojciechowska, Zofia Balwierz, and Marek L. Kowalski
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allergenic pollen ,pollen concentration ,Alnus ,Corylus ,Betula ,Poaceae ,Ambrosia ,Artemisia ,Agriculture (General) ,S1-972 - Abstract
Recent studies suggest that climate change can influence plant reproductive systems and have an impact on the increase in allergenic pollen in atmospheric air; highly allergenic pollen may intensify the allergic response in people. The aim of our study was to evaluate the seasonal dynamic concentration of the most allergenic pollen taxa, i.e., the following trees: Alnus, Corylus, Betula, and herbaceous plants: grasses (Poaceae), Artemisia, and Ambrosia, in the long-term period of 2003–2013 in the city of Lodz, Poland. Weekly airborne pollen concentrations were evaluated with a volumetric Lansoni pollen trap. The beginning and the end of the season were calculated by the 98% method. The birch (Betula) pollen was at the highest level and accounted for 79%, followed by alder (Alnus) – 19%, and hazel (Corylus) – 2%. Among the herbaceous taxa, grasses (Poaceae) pollen dominated – 79%, followed by mugwort (Artemisia) – 18%, and ragweed (Ambrosia) – 3%. Our findings indicate a lack of qualitative and quantitative change in the pollen produced over the 11-year period.
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- 2016
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20. Toll-Like Receptor Agonists Modulate Wound Regeneration in Airway Epithelial Cells
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Anna Lewandowska-Polak, Małgorzata Brauncajs, Marzanna Jarzębska, Małgorzata Pawełczyk, Marcin Kurowski, Maciej Chałubiński, Joanna Makowska, and Marek L. Kowalski
- Subjects
airway epithelium ,wound repair ,TLRs ,poly(I:C) ,LPS ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Background: Impaired regeneration of airway epithelium may lead to persistence of inflammation and remodelling. Regeneration of injured epithelium is a complex phenomenon and the role of toll-like receptors (TLRs) in the stimulation of respiratory virus products in this process has not been established. Objective: This study was undertaken to test the hypothesis that the wound repair process in airway epithelium is modulated by microbial products via toll-like receptors. Methods: Injured and not-injured bronchial epithelial cells (ECs) (BEAS-2B line) were incubated with the TLR agonists poly(I:C), lipopolisacharide (LPS), allergen Der p1, and supernatants from virus-infected epithelial cells, either alone or in combination with TLR inhibitors. Regeneration and immune response in injured and not-injured cells were studied. Results: Addition of either poly(I:C) or LPS to ECs induced a marked inhibition of wound repair. Supernatants from RV1b-infected cells also decreased regeneration. Preincubation of injured and not-injured ECs with TLR inhibitors decreased LPS and poly(I:C)-induced repair inhibition. TGF-β and RANTES mRNA expression was higher in injured ECs and IFN-α, IFN-β, IL-8, and VEGF mRNA expression was lower in damaged epithelium as compared to not-injured. Stimulation with poly(I:C) increased IFN-α and IFN-β mRNA expression in injured cells, and LPS stimulation decreased interferons mRNA expression both in not-injured and injured ECs. Conclusion: Regeneration of the airway epithelium is modulated by microbial products via toll-like receptors.
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- 2018
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21. RANTES and Chemotactic Activity in Synovial Fluids From Patients With Rheumatoid Arthritis and Osteoarthritis
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Joanna Stanczyk, Marek L. Kowalski, Janina Grzegorczyk, Barbara Szkudlinska, Marzanna Jarzebska, Marek Marciniak, and Marek Synder
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Pathology ,RB1-214 - Abstract
A massive accumulation of inflammatory cells in synovial tissues is a major pathological feature of rheumatoid arthritis (RA). Neutrophiles dominate synovial fluid while rheumatoid synovium is infiltrated with mononuclear cells. Mechanisms regulating influx of particular subpopulations of leukocytes into articular cavity and synovium compartment are not completely defined. An increasing amount of data supports a crucial role of a C-C chemokine RANTES in the RA pathogenesis. Our objective is to evaluate chemotactic activity for neutrophils (NCA), lymphocytes (LCA), and monocytes (MoCA) in SFs obtained from patients with RA and osteoarthritis (OA). We also aimed to characterise the relation between chemotactic activity, RANTES, and percentage distribution of leukocytes in SF. SFs from 11 patients with RA and 6 with OA were included in the study. Modified microchamber Boyden method was employed to assess chemotactic activity. Cytological and biochemical analysis of SF was performed. RANTES was measured with ELISA. Rheumatoid SFs were rich in cells with predominance of neutrophiles while osteoarthritic fluids were lymphocytic. RA SFs were also characterised by increased lactoferrin level. Both NCA and LCA were higher in SF from patients with RA (62±12 and 24±6 cells/HPF, resp) as compared to patients with OA (23±6; P
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- 2005
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22. Tumour necrosis factor-α polymorphism as one of the complex inherited factors in pemphigus
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Jolanta Dorota Torzecka, Joanna Narbutt, Anna Sysa-jedrzejowska, Maciej Borowiec, Anetta Ptasinska, Grzegorz Woszczek, and Marek L. Kowalski
- Subjects
Pathology ,RB1-214 - Abstract
The aim of our study was to analyse a significance of tumour necrosis factor (TNF)-α promoter gene polymorphisms in relation to the HLA-DR locus in genetic predisposition to pemphigus. TNF-α gene polymorphisms in position -238 and -308 were identified using a modified polymerase chain reaction-restriction fragment length polymorphism method in 53 patients with pemphigus (38 with pemphigus vulgaris, 15 with pemphigus foliaceus) and 87 healthy controls. The HLA-DRB1 locus was typed using the polymerase chain reaction SSO method in all the patients and 152 population controls.
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- 2003
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23. Increased apoptosis of peripheral blood mononuclear cells in patients with perennial allergic asthma/rhinitis: relation to serum markers of apoptosis
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Janina Grzegorczyk, Marek L. Kowalski, Anna Pilat, and Jolanta Iwaszkiewicz
- Subjects
Pathology ,RB1-214 - Abstract
Background: The goal of our study was to examine spontaneous and stimulated apoptosis of peripheral blood MNC from allergic patients, sensitized to Der p I antigen as compared to cells from non-atopic subjects. Furthermore we aimed to investigate which populations of mononuclear cells (lymphocytes, monocytes) undergo the apoptosis and to determine relations between apoptosis and serum levels of sFas/APO-1, ICE/caspase-1 or TNF-α.
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- 2002
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24. <scp>miRNA</scp> expression in serum and <scp>PBMCs</scp> isolated from middle‐aged and elderly patients during asthma exacerbation
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Aleksandra Wardzyńska, Małgorzata Pawełczyk, Joanna Rywaniak, Joanna S. Makowska, Marek L. Kowalski, and Maciej Chałubiński
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Microbiology (medical) ,Immunology and Allergy ,General Medicine ,Pathology and Forensic Medicine - Published
- 2023
25. Allergy clinic patients’ drug hypersensitivity
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Karolina Frachowicz-Guerreiro, Aleksandra Wardzyńska, and Marek L. Kowalski
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Drug Hypersensitivity ,Pulmonary and Respiratory Medicine ,Anti-Inflammatory Agents, Non-Steroidal ,Immunology ,Humans ,Immunology and Allergy ,General Medicine ,beta-Lactams ,Ambulatory Care Facilities ,Anti-Bacterial Agents ,Retrospective Studies ,Skin Tests - Abstract
Background: Drug hypersensitivity reaction (DHR) is a common reason for an allergology consultation, during which it is not only necessary to gather a thorough medical history, but also to propose and perform diagnostic tests. Objectives: The aim of the study was to retrospectively assess the patients with a profile of preliminary drug hypersensitivity diagnosis, the usefulness of NSAID hypersensitivity classification in outpatient practice, and to analyze the results of skin, provocation, and drug tolerance tests performed in Immunology and Allergy Clinic patients. Methods: Around 501 medical records of patients referred to the academic allergy outpatient clinic from 2011 to 2019, and had a preliminary drug hypersensitivity diagnosis were analyzed. The diagnostic and drug tolerance tests results carried out in 269 patients of the Clinic from 2009 to 2019 were then evaluated. Results: Among the patients referred due to suspected drug hypersensitivity, the majority (n=338, 67.5%) were believed to be hypersensitive to NSAIDs and antibiotics (n=272, 54.3%). In patients with hypersensitivity to NSAIDs, the mixed pattern was the most prevalent (n=73, 21.6%), followed by NECD (n=64, 18.9%) and NIUA (n=55, 16.3%). The second most common drug causing DHR were the antibiotics, mainly β-lactams (n=160, 58.8%), followed by macrolides (n=35, 12.9%). In hypersensitivity caused due to β-lactams, the delayed form was predominant (n=24, 15%) with manifested skin symptoms (n=74, 46.3%). Non-steroidal anti-inflammatory drugs (n=21, 42.9%), followed by antibiotics (n=11, 22.5%) were the commonest causes of anaphylaxis, as reported by 49 patients. Conclusion: The study shows that a majority of patients with suspected drug hypersensitivity can be classified under the hypersensitivity umbrella based on their medical history, which is the basis for further diagnostic process.
- Published
- 2022
26. MicroRNA expression profile in peripheral blood mononuclear cells of asthmatic patients and healthy individuals: The effect of age and ex vivo rhinovirus exposure
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Maciej Chałubiński, Marek L. Kowalski, Joanna Rywaniak, Małgorzata Pawełczyk, Joanna Makowska, Joanna Jamroz-Brzeska, and Aleksandra Wardzyńska
- Subjects
Picornaviridae Infections ,Rhinovirus ,business.industry ,Immunology ,Disease ,Immunosenescence ,medicine.disease_cause ,Peripheral blood mononuclear cell ,Asthma ,Proinflammatory cytokine ,MicroRNAs ,Immune system ,Ageing ,Leukocytes, Mononuclear ,Humans ,Immunology and Allergy ,Medicine ,business ,Ex vivo - Abstract
Ageing of the immune system (or 'immunosenescence') is associated with higher mortality due to infectious diseases and reduced response to vaccines. Dysregulation of the cellular response may be associated with an increased incidence of neoplasms, while an elevated level of proinflammatory cytokines, produced mostly by innate cells, contributes to age-related inflammatory diseases including osteoporosis, type 2 diabetes, atherosclerosis or Alzheimer's disease [1].
- Published
- 2021
27. Human rhinovirus 16 induces antiviral and inflammatory response in the human vascular endothelium
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Adrian Gajewski, Aleksandra Likońska, Maciej Chałubiński, Marek L. Kowalski, Aleksandra Szulc, Mateusz Gawrysiak, and Małgorzata Pawełczyk
- Subjects
0301 basic medicine ,Microbiology (medical) ,Rhinovirus ,Endothelium ,viruses ,Inflammation ,medicine.disease_cause ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Human Umbilical Vein Endothelial Cells ,medicine ,Humans ,Immunology and Allergy ,Receptors, Immunologic ,Receptor ,Chemokine CCL5 ,Picornaviridae Infections ,Chemistry ,virus diseases ,MDA5 ,Interferon-beta ,General Medicine ,Protein kinase R ,Toll-Like Receptor 3 ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,TLR3 ,Immunology ,DEAD Box Protein 58 ,Respiratory epithelium ,Endothelium, Vascular ,medicine.symptom - Abstract
The effect of rhinovirus on airway epithelium is very well described. However, its influence on the vascular endothelium is unknown. The current study assesses the effect of rhinovirus HRV16 on the antiviral and inflammatory response in the human vascular endothelial cells (ECs). HRV16 increased IFN-β, RANTES, and IP-10 mRNA expression and protein release. HRV16 copy number in ECs reached maximal value 10 h after incubation. Increase in virus copies was accompanied by the enhancement of Toll- and RIG-I-like receptors: TLR3, RIG-I, and MDA5. Additionally, HRV16 increased OAS-1 and PKR mRNA expression, enzymes responsible for virus degradation and inhibition of replication. ICAM-1 blockade decreased HRV16 copy number in ECs and inhibited IFN-β, RANTES, IP-10, OAS1, PKR, TLR3, RIG-I, and MDA5 mRNA expression increase upon subsequent induction with HRV16. The vascular endothelium may be infected by human rhinovirus and generate antiviral and inflammatory innate response. Results of the study indicate the possible involvement of the vascular endothelium in the immunopathology of rhinoviral airway infections.
- Published
- 2020
28. Human rhinovirus HRV16 impairs barrier functions and regeneration of human lung vascular endothelium
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Sylwia Michlewska, Adrian Gajewski, Aleksandra Likońska, Marek L. Kowalski, Robert Szewczyk, Maciej Chałubiński, Magdalena Chmiela, and Mateusz Gawrysiak
- Subjects
Lung ,Rhinovirus ,Endothelium ,business.industry ,Regeneration (biology) ,Immunology ,medicine.disease_cause ,Human lung ,Vascular endothelium ,medicine.anatomical_structure ,medicine ,Cancer research ,Humans ,Regeneration ,Immunology and Allergy ,Endothelium, Vascular ,business - Published
- 2020
29. Angioedema. Interdisciplinary diagnostic and therapeutic recommendations of the Polish Dermatological Society (PTD) and Polish Society of Allergology (PTA)
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Beata Krecisz, Cezary Kowalewski, Marek L. Kowalski, Grzegorz Porebski, Magdalena Lange, Elżbieta Grubska-Suchanek, Lidia Rudnicka, Aneta Szczerkowska-Dobosz, Aleksandra Wilkowska, Radosław Śpiewak, Zbigniew Doniec, Małgorzata Sokołowska-Wojdyło, Rafał Czajkowski, Tomasz Matuszewski, Roman Nowicki, Malgorzata Olszewska, Karina Jahnz-Różyk, Wioletta Barańska-Rybak, Marek Kulus, Magdalena Trzeciak, and Joanna Narbutt
- Subjects
Allergy ,medicine.medical_specialty ,berinert ,landelumab ,Acquired angioedema ,Lanadelumab ,Dermatology ,Berinert ,ecallantide ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,Ecallantide ,Special Article ,0302 clinical medicine ,rituximab ,Icatibant ,immune system diseases ,firazyr ,medicine ,Immunology and Allergy ,cardiovascular diseases ,skin and connective tissue diseases ,Internal medicine ,General Environmental Science ,Angioedema ,business.industry ,angioedema ,icatibant ,food and beverages ,medicine.disease ,RC31-1245 ,chemistry ,RL1-803 ,Hereditary angioedema ,Etiology ,General Earth and Planetary Sciences ,Medicine ,Rituximab ,Ruconest ,Allergists ,medicine.symptom ,business ,Firazyr ,medicine.drug ,ruconest - Abstract
Angioedema is a non-inflammatory oedema of the subcutaneous tissue and/or mucosal membranes. It most commonly coexists with urticaria wheals and is considered to be a deep form of urticaria. Less commonly, it occurs in isolation and can take two basic forms: acquired angioedema and hereditary angioedema. Currently, there are 4 defined types of acquired angioedema and 7 types of hereditary angioedema. Treatment of angioedema depends on its form and etiological factors. Especially the genetic form, i.e. hereditary angioedema, is a considerable challenge for medical specialists, particularly dermatologists and allergists.
- Published
- 2020
30. Physical exercise, immune response, and susceptibility to infections-current knowledge and growing research areas
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Marcin Kurowski, Sven Seys, Matteo Bonini, Stefano Del Giacco, Luis Delgado, Zuzana Diamant, Marek L. Kowalski, André Moreira, Maia Rukhadze, and Mariana Couto
- Subjects
Inflammation ,Immunity, Cellular ,SARS-CoV-2 ,Immunology ,Immunology and Allergy ,COVID-19 ,Humans ,Exercise ,Respiratory Tract Infections - Abstract
This review presents state-of-the-art knowledge and identifies knowledge gaps for future research in the area of exercise-associated modifications of infection susceptibility. Regular moderate-intensity exercise is believed to have beneficial effects on immune health through lowering inflammation intensity and reducing susceptibility to respiratory infections. However, strenuous exercise, as performed by professional athletes, may promote infection: in about half of athletes presenting respiratory symptoms, no causative pathogen can be identified. Acute bouts of exercise enhance the release of pro-inflammatory mediators, which may induce infection-like respiratory symptoms. Relatively few studies have assessed the influence of regularly repeated exercise on the immune response and systemic inflammation compared to the effects of acute exercise. Additionally, ambient and environmental conditions may modify the systemic inflammatory response and infection susceptibility, particularly in outdoor athletes. Both acute and chronic regular exercise influence humoral and cellular immune response mechanisms, resulting in decreased specific and non-specific response in competitive athletes. The most promising areas of further research in exercise immunology include detailed immunological characterization of infection-prone and infection-resistant athletes, examining the efficacy of nutritional and pharmaceutical interventions as countermeasures to infection symptoms, and determining the influence of various exercise loads on susceptibility to infections with respiratory viruses, including SARS-CoV-2. By establishing a uniform definition of an "elite athlete," it will be possible to make a comparable and straightforward interpretation of data from different studies and settings.
- Published
- 2022
31. IL-33 prevents the enhancement of AP-N, DPP4, and ACE2 expression induced by rhinovirus HRV16 in the human lung endothelium-potential implications for coronaviral airway infections
- Author
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Izabela Gulbas, Adrian Gajewski, Mateusz Gawrysiak, Robert Szewczyk, Aleksandra Likońska, Sylwia Michlewska, Marek L. Kowalski, and Maciej Chałubiński
- Subjects
Rhinovirus ,Dipeptidyl Peptidase 4 ,Immunology ,Immunology and Allergy ,Humans ,Angiotensin-Converting Enzyme 2 ,Endothelium ,Interleukin-33 ,Lung - Published
- 2022
32. Cutaneous Hypersensitivity Reactions to Analgesics and Nonsteroidal Anti-Inflammatory Drugs
- Author
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Marek L. Kowalski and Aleksandra Wardzynska
- Published
- 2022
33. Predicting food allergy: The value of patient history reinforced
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Ronald van Ree, Paraskevi Xepapadaki, L. Barreales, Peter Burney, Marek L. Kowalski, Paco M J Welsing, Monika Jedrzejczak-Czechowicz, Tihomir B. Mustakov, Michael Clausen, Todor A. Popov, E. N. Clare Mills, André C. Knulst, Nikolaos G. Papadopoulos, Serge A. Versteeg, Tanya Kralimarkova, Ruta Dubakiene, James Potts, Barbara Ballmer-Weber, Cristina Fernández-Pérez, Harmieke van Os-Medendorp, Ischa Kummeling, Christian Bieli, Montserrat Fernandez-Rivas, Sarah A. Lyons, Thuy-My Le, University of Zurich, Lyons, Sarah A, Experimental Immunology, APH - Global Health, APH - Personalized Medicine, Ear, Nose and Throat, and AII - Inflammatory diseases
- Subjects
Adult ,Male ,food sensitization ,0301 basic medicine ,medicine.medical_specialty ,Allergy ,Demographics ,Immunology ,Population ,610 Medicine & health ,Plant foods ,Food Allergy and Gastrointestinal Disease ,03 medical and health sciences ,0302 clinical medicine ,Food allergy ,Internal medicine ,Prevalence ,Humans ,Immunology and Allergy ,Medicine ,Medical history ,Child ,education ,Asthma ,food allergy ,2403 Immunology ,education.field_of_study ,business.industry ,Reproducibility of Results ,10177 Dermatology Clinic ,prediction ,Allergens ,medicine.disease ,Comorbidity ,Europe ,030104 developmental biology ,030228 respiratory system ,patient history ,10036 Medical Clinic ,2723 Immunology and Allergy ,Original Article ,ORIGINAL ARTICLES ,business ,Food Hypersensitivity - Abstract
Background EAACI guidelines emphasize the importance of patient history in diagnosing food allergy (FA) and the need for studies investigating its value using standardized allergy‐focused questionnaires. Objective To determine the contribution of reaction characteristics, allergic comorbidities and demographics to prediction of FA in individuals experiencing food‐related adverse reactions. Methods Adult and school‐age participants in the standardized EuroPrevall population surveys, with self‐reported FA, were included. Penalized multivariable regression was used to assess the association of patient history determinants with “probable” FA, defined as a food‐specific case history supported by relevant IgE sensitization. Results In adults (N = 844), reproducibility of reaction (OR 1.35 [95% CI 1.29‐1.41]), oral allergy symptoms (OAS) (4.46 [4.19‐4.75]), allergic rhinitis (AR) comorbidity (2.82 [2.68‐2.95]), asthma comorbidity (1.38 [1.30‐1.46]) and male sex (1.50 [1.41‐1.59]) were positively associated with probable FA. Gastrointestinal symptoms (0.88 [0.85‐0.91]) made probable FA less likely. The AUC of a model combining all selected predictors was 0.85 after cross‐validation. In children (N = 670), OAS (2.26 [2.09‐2.44]) and AR comorbidity (1.47 [CI 1.39‐1.55]) contributed most to prediction of probable FA, with a combined cross‐validation‐based AUC of 0.73. When focusing on plant foods, the dominant source of FA in adults, the pediatric model also included gastrointestinal symptoms (inverse association), and the AUC increased to 0.81. Conclusions In both adults and school‐age children from the general population, reporting of OAS and of AR comorbidity appear to be the strongest predictors of probable FA. Patient history particularly allows for good discrimination between presence and absence of probable plant FA., In support of expert opinion, data collected in a standardized manner all across Europe reveal that information available from patient history can accurately predict IgE sensitization corresponding to a food‐specific case history (probable FA). OAS and AR comorbidity are strongly associated with presence of FA and GI symptoms with absence of FA (particularly plant FA) in adults and children. Abbreviations: AA, allergic asthma; AR, allergic rhinitis; FA, food allergy; GI, gastrointestinal; OAS, oral allergy symptoms
- Published
- 2020
34. Toward personalization of asthma treatment according to trigger factors
- Author
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Luis Caraballo, Susanne Greber-Platzer, W. Pohl, Bulent Enis Sekerel, Valérie Siroux, Elena S. Fedenko, Natalia Ilina, Paolo Maria Matricardi, Harald Renz, Petra Pazderova, Zhanat Ispayeva, Elopy Sibanda, Kian Fan Chung, Nikolaos G. Papadopoulos, Hugo Van Bever, Marianne van Hage, Alexander Emelyanov, S. G. Makarova, Roxana S. Bumbacea, Carmen Panaitescu, Erika von Mutius, Faith H. A. Osier, Sebastian L. Johnston, Jin Lyu Sun, Gary W.K. Wong, R S Fassakhov, Ludmila P. Sizyakina, Kristina Borochova, Jiu-Yao Wang, Evgeny Beltyukov, Zhongshan Gao, Kari C. Nadeau, Antonina Karsonova, Marco Idzko, Snezhana Bychkovskaya, A.N. Pampura, Katarzyna Niespodziana, Peter Errhalt, Tatiana Baranovskaya, Dmitry Kudlay, M. Gotua, Natalia Astafyeva, Thomas Schlederer, Marek L. Kowalski, Rezeda Fayzullina, Musa Khaitov, Tetiana Umanets, Ksenja Riabova, Leyla Namazova-Baranova, Pedro Giavina-Bianchi, Ruby Pawankar, Mohamed-Ridha Barbouche, Zsolt Szépfalusi, Sergii Zaikov, Hae-Sim Park, Guillermo Horacio Docena, Irina Evsegneeva, Mihaela Zidarn, Michael Levin, Adnan Custovic, Jean Bousquet, Elena Kovzel, Paul M. O'Byrne, Daria Fomina, Oleksandr Nazarenko, Elena Borzova, Thomas Eiwegger, Olga Naumova, Gunilla Hedlin, Omer Kalayci, Rudolf Valenta, Vanitha Sampath, Angelika Berger, Margarita Vasileva, Alexander Karaulov, Graduate School, Medical Microbiology and Infection Prevention, AII - Inflammatory diseases, Salvy-Córdoba, Nathalie, Medizinische Universität Wien = Medical University of Vienna, Saratov State Medical University, Partenaires INRAE, Belarusian Medical Academy of Post-Graduate Education [Minsk] (BelMAPGE), Laboratoire d'immunologie clinique [Institut Pasteur de Tunis], Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), South Ural State Medical University, Russian Medical Academy for Continuous Medical Education [Moscow], Pirogov Russian National Research Medical University, Sechenov First Moscow State Medical University, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Contre les MAladies Chroniques pour un VIeillissement Actif en Languedoc-Roussillon (MACVIA-LR), Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Nîmes (CHU Nîmes)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-European Innovation Partnership on Active and Healthy Ageing Reference Site (EIP on AHA), Commission Européenne-Commission Européenne-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Humboldt University Of Berlin, Berlin Institute of Health (BIH), University of Medicine and Pharmacy 'Carol Davila' Bucharest (UMPCD), Krasnoyarsk State Medical University (KrasSMU), Universidad de Cartagena [Cartagena de Indias], National Heart and Lung Institute [London] (NHLI), Imperial College London-Royal Brompton and Harefield NHS Foundation Trust, Universidad Nacional de la Plata [Argentine] (UNLP), The Hospital for sick children [Toronto] (SickKids), North-Western State Medical University [St Petersburg, Russia], Krems University Hospital, Kazan Federal University (KFU), Bashkir State University (BASHEDU), NRC Institute of immunology FMBA, Moscow Russian federation, Zhejiang Gongshang University [Hangzhou] (ZJSU), Universidade de São Paulo = University of São Paulo (USP), Tbilisi State University, Astrid Lindgren Children's Hospital, Karolinska University Hospital [Stockholm], Karolinska Institutet [Stockholm], Al-Farabi Kazakh National University [Almaty] (KazNU), Faculty of Medicine [Hacettepe University], Hacettepe University = Hacettepe Üniversitesi, Nazarbayev University [Kazakhstan], Medical University of Łódź (MUL), University of Cape Town, Dmitry Rogachev National Research Center of Pediatric Hematology, Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Sean N. Parker Center for Allergy and Asthma Research [Stanford], Stanford Medicine, Stanford University-Stanford University, Pirogov Russian National Reasearch Medical University Moscow, National Academy of Medical Sciences of Ukraine, Shupyk National Medical Academy of Postgraduate Education [Kiev] (SNMAPE), Michael DeGroote School of Medicine [Hamilton, ON, Canada], Faculty of Health Sciences [Hamilton, ON, Canada], McMaster University [Hamilton, Ontario]-McMaster University [Hamilton, Ontario], KEMRI-Wellcome Trust Research Programme (KWTRP), Pius Branzeu Clinical Emergency Hospital (OncoGen), University of Manchester [Manchester], National and Kapodistrian University of Athens (NKUA), Ajou University, Nippon Medical School [Tokyo, Japon], Hietzing Hospital, Karl Landsteiner Institute for Dermatological Research, Landesklinikum St. Poelten, University of Giessen and Marburg Lung Center [Gießen, Germany] (UGMLC), German Center for Lung Research, University of Zimbawe [Harare] (UZ), University of Zimbawe, National University of Science and Technology [Bulawayo], Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Southern Federal University [Rostov-on-Don] (SFEDU), Peking Union Medical College Hospital [Beijing] (PUMCH), Chinese Academy of Medical Sciences and Peking Union Medical College, National University of Singapore (NUS), Center of Allergology and Clinical Immunology, Dr von Hauner Children's Hospital [Munich, Germany], Ludwig-Maximilians-Universität München (LMU), Helmholtz Centre Munich, German Centre for Lung Research, National Cheng Kung University Hospital [Tainan], The Chinese University of Hong Kong [Hong Kong], Respiratory and Allergic Diseases [Golnik, Slovenia], University Clinic of Respiratory and Allergic Diseases Golnik, University of Ljubljana, and Karl Landsteiner University of Health Sciences - Karl Landsteiner Privatuniversität für Gesundheitswissenschaften [Krems an der Donau, Austria]
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0301 basic medicine ,Allergy ,MESH: Asthma ,Rhinovirus ,Asthma treatment ,CHILDREN ,EPITOPE ,Disease ,Microarray ,medicine.disease_cause ,sopenje ,alergeni ,Personalization ,Atopy ,0302 clinical medicine ,immune system diseases ,astma ,Immunology and Allergy ,MESH: Animals ,Precision Medicine ,RISK ,MESH: Rhinovirus ,respiratorni znaki in simptomi ,analiza mikromrež ,Allergen ,alergija in imunologija ,SENSITIZATION ,personalizirana medicina ,Wheeze ,rhinovirus ,allergy and immunology ,INFECTIONS ,1107 Immunology ,wheeze ,ATOPY ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,medicine.symptom ,Life Sciences & Biomedicine ,Bioquímica ,medicine.medical_specialty ,MESH: Allergens ,[SDV.IMM] Life Sciences [q-bio]/Immunology ,precision medicine ,Immunology ,udc:616-097 ,MESH: Precision Medicine ,03 medical and health sciences ,[SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics ,rinovirus ,medicine ,Animals ,Humans ,allergens ,IMMUNOTHERAPY ,Intensive care medicine ,Asthma ,[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics ,MESH: Humans ,Science & Technology ,business.industry ,Allergens ,asthma ,respiratory ,medicine.disease ,respiratory tract diseases ,signs and symptoms ,030104 developmental biology ,ANTIBODY ,030228 respiratory system ,Ciencias Médicas ,MESH: Biomarkers ,microarray analysis ,business ,Biomarkers ,RESPONSES - Abstract
Asthma is a severe and chronic disabling disease affecting more than 300 million people worldwide. Although in the past few drugs for the treatment of asthma were available, new treatment options are currently emerging, which appear to be highly effective in certain subgroups of patients. Accordingly, there is a need for biomarkers that allow selection of patients for refined and personalized treatment strategies. Recently, serological chip tests based on microarrayed allergen molecules and peptides derived from the most common rhinovirus strains have been developed, which may discriminate 2 of the most common forms of asthma, that is, allergen- and virus-triggered asthma. In this perspective, we argue that classification of patients with asthma according to these common trigger factors may open new possibilities for personalized management of asthma., La lista completa de autores que integran el documento puede consultarse en el archivo., Facultad de Ciencias Exactas
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- 2020
35. Associations of ficolins and mannose-binding lectin with acute myeloid leukaemia in adults
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Agnieszka Szala-Poździej, Gabriela Gajek, Agnieszka Wierzbowska, Marek L. Kowalski, Jens C. Jensenius, Anna Sokolowska, Misao Matsushita, Maciej Cedzynski, Steffen Thiel, Mateusz Nowicki, Olga Brzezińska, Aleksandra Gołos, Anna Wolska-Washer, Anna S. Świerzko, Mateusz Michalski, and Krzysztof Jamroziak
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Adult ,Male ,0301 basic medicine ,Genotype ,medicine.medical_treatment ,Science ,Immunology ,Malignancy ,Mannose-Binding Lectin ,Article ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,Polymorphism (computer science) ,Lectins ,Biomarkers, Tumor ,medicine ,Humans ,Genetic Predisposition to Disease ,Genotyping ,Alleles ,Multiple myeloma ,Aged ,Mannan-binding lectin ,Aged, 80 and over ,Chemotherapy ,Polymorphism, Genetic ,Multidisciplinary ,biology ,business.industry ,Lectin ,Complement Pathway, Mannose-Binding Lectin ,Middle Aged ,medicine.disease ,Lymphoma ,Leukemia, Myeloid, Acute ,030104 developmental biology ,Risk factors ,Mannose-Binding Protein-Associated Serine Proteases ,030220 oncology & carcinogenesis ,biology.protein ,Medicine ,Female ,business ,Biomarkers - Abstract
We investigated clinical associations of ficolins and mannose-binding lectin (MBL) in 157 patients suffering from acute myeloid leukaemia (AML). Concentrations of ficolin-1, ficolin-2, ficolin-3 and MBL (before chemotherapy) in serum were determined as were selected polymorphisms of the corresponding genes (FCN1, FCN2, FCN3 and MBL2). The control group (C) consisted of 267 healthy unrelated individuals. Median level of ficolin-1 in patients was lower (p p p p = 0.0016, respectively) compared with controls. These findings were generally associated with AML itself, however the highest MBL levels predicted higher risk of severe hospital infections (accompanied with bacteremia and/or fungaemia) (p = 0.012) while the lowest ficolin-1 concentrations tended to be associated with prolonged (> 7 days) fever (p = 0.026). Genotyping indicated an association of G/G homozygosity (corresponding to FCN1 gene − 542 G > A polymorphism) with malignancy [p = 0.004, OR = 2.95, 95% CI (1.41–6.16)]. Based on ROC analysis, ficolin-1, -2 and -3 may be considered candidate supplementary biomarkers of AML. Their high potential to differentiate between patients from non-malignant controls but also from persons suffering from other haematological cancers (multiple myeloma and lymphoma) was demonstrated.
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- 2020
36. Position statement of expert panel of the Polish Allergology Society on the management of patients with bronchial asthma and allergic diseases during SARS-Cov-2 pandemics
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Marek L. Kowalski, Jerzy Kruszewski, Maciej Chałubiński, Magdalena Czarnecka-Operacz, Marek Niedoszytko, Anna Bręborowicz, Marek Kulus, Roman Nowicki, Zbigniew Bartuzi, Barbara Rogala, Marcin Moniuszko, and Marita Nittner-Marszalska
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Position statement ,Allergen immunotherapy ,medicine.medical_specialty ,Allergy ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Disease ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,030228 respiratory system ,Pandemic ,medicine ,General Earth and Planetary Sciences ,030212 general & internal medicine ,Allergists ,Intensive care medicine ,business ,General Environmental Science ,Asthma - Abstract
The document prepared by the expert panel of allergy/immunology specialists representing Polish Allergy Society intends to provide allergists with recommendations with respect to proper management of allergic patients during SARS-CoV-2 pandemics Allergist should aim at reducing patient’s social interactions by replacing face-to-face visits with communication via telephone or internet In parallel allergist is responsible for assuring that the patient is receiving proper care of underlying allergic disease The experts panel provides eighteen recommendations for management of patients with asthma and allergic diseases during pandemics, indicating that in majority of patients asthma treatment (including inhaled corticosteroids) and allergy treatment should be continued Specific recommendations for treatment with allergen immunotherapy and with biologics are discussed in details
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- 2020
37. The profile of respiratory pathogens in induced sputum of elderly and non-elderly asthmatics
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Joanna Makowska, Aleksandra Wardzyńska, Anna Głobińska, Marek L. Kowalski, and Małgorzata Pawełczyk
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Atypical bacteria ,Immunology ,lcsh:Medicine ,medicine.disease_cause ,elderly ,03 medical and health sciences ,FEV1/FVC ratio ,0302 clinical medicine ,medicine ,Immunology and Allergy ,Eosinophilia ,Respiratory system ,Asthma ,business.industry ,lcsh:R ,pathogens ,asthma ,medicine.disease ,induced sputum ,Chlamydophila pneumoniae ,Exhaled nitric oxide ,Sputum ,Clinical Immunology ,medicine.symptom ,business ,030215 immunology - Abstract
Introduction Respiratory pathogens are thought to be involved in the pathogenesis and exacerbations of asthma at all ages; however, little is known about the airway microbiome in the elderly. Aim of the study To identify respiratory pathogens in the induced sputum (IS) of elderly asthmatics, and to determine the association between pathogens and the markers of asthma activity. Material and methods Twenty-nine subjects with stable asthma, 15 above 65 years of age and 14 aged 30-49 years, underwent clinical evaluation, fractional exhaled nitric oxide measurement, and sputum induction. Pathogens were detected by multiplex reverse transcription polymerase chain reaction. The periostin concentration of IS supernatants was measured by enzyme-linked immunosorbent assay. Serum eosinophil cationic protein and total IgE levels were measured by ImmunoCAP. Results Elderly patients, as compared to non-elderly, had significantly higher eosinophilia in IS, although other markers of eosinophilic inflammation were comparable. Half of the subjects were positive for Haemophilus influenzae. Chlamydophila pneumoniae was found in two subjects. Respiratory viruses were detected in more than 70% of patients. The detection rates and profiles of atypical bacteria and respiratory viruses were similar in both groups. Only in the elderly asthmatics was influenza A positivity associated with lower predicted FVC%, RSV A positivity connected with decreased tIgE concentration, and RSV B positivity related to a lower percentage of lymphocytes in IS. Conclusions Despite the existence of differences in some clinical and inflammatory characteristics of asthma between elderly and non-elderly asthmatics, the pathogen detection rates in the IS from the two groups are similar.
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- 2020
38. Allergic Rhinitis
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Marek L. Kowalski and Marek L. Kowalski
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- 2012
39. IL-33 may augment the effect of rhinovirus HRV16 on the inflammatory activity of human lung vascular endothelium – possible implication for asthma exacerbations
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Robert Szewczyk, Marek L. Kowalski, Maciej Chałubiński, Adrian Gajewski, and Mateusz Gawrysiak
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Interleukin 33 ,Vascular endothelium ,Asthma exacerbations ,medicine.anatomical_structure ,business.industry ,Immunology ,medicine ,Rhinovirus ,Augment ,medicine.disease_cause ,business ,Human lung - Published
- 2021
40. Asthma in elite athletes – a non-Type 2 disease
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Marcin Kurowski, Sergio Bonini, Pekka Malmberg, Katja Radon, Sofia Vakali, Søren Malte Rasmussen, Trine Stensrud, Luís Delgado, Nikos Papadoupolous, Erik Sören Halvard Hansen, Marek L. Kowalski, Tari Haahtela, Mikko Voutilainen, André Moreira, Franchek Drobnik, Christina Gratziou, Matteo Bonini, Vibeke Backer, and Jean Bousquet
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medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Elite athletes ,Disease ,business ,medicine.disease ,Asthma - Published
- 2021
41. Interferon-dependant and immune cell-based mechanisms of antiviral resistance of the human lung vascular endothelium infected with rhinovirus HRV16
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Aleksandra Likońska, Mateusz Gawrysiak, Adrian Gajewski, Mateusz Kobierecki, Michał Szymański, Maciej Chałubiński, Izabela Gulbas, Marek L. Kowalski, Robert Szewczyk, and Sylwia Michlewska
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business.industry ,Antiviral resistance ,medicine.disease_cause ,Human lung ,Vascular endothelium ,medicine.anatomical_structure ,Immune system ,Interferon ,Immunology ,medicine ,Rhinovirus ,business ,Cell based ,medicine.drug - Published
- 2021
42. Human rhinovirus causes increase in angiogenetic activity of lung endothelial cells – potential involvement in enhanced vasculature in asthmatics
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Adrian Gajewski, Robert Szewczyk, Maciej Chałubiński, Kinga Klimczak, Mateusz Gawrysiak, Izabela Gulbas, Aleksandra Likońska, and Marek L. Kowalski
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Lung ,medicine.anatomical_structure ,business.industry ,Immunology ,medicine ,Rhinovirus ,business ,medicine.disease_cause - Published
- 2021
43. The effect of IL-33 and IL-25 on the rhinovirus-induced susceptibility of the lung vascular endothelium to SARS-CoV-2, MERS and 229E coronaviral infections
- Author
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Mateusz Gawrysiak, Robert Szewczyk, Maciej Chałubiński, Izabela Gulbas, Marek L. Kowalski, and Adrian Gajewski
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Vascular endothelium ,Interleukin 33 ,Lung ,medicine.anatomical_structure ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Immunology ,Medicine ,Rhinovirus ,business ,medicine.disease_cause - Published
- 2021
44. Physical exercise, immune response and susceptibility to infections -- current knowledge and growing research areas
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Matteo Bonini, André Moreira, Zuzana Diamant, Stefano Del Giacco, Sven Seys, Maia Rukhadze, Luís Delgado, Marek L. Kowalski, Marcin Kurowski, and Mariana Couto
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medicine.medical_specialty ,biology ,business.industry ,Research areas ,Athletes ,Strenuous exercise ,Psychological intervention ,Inflammation ,Physical exercise ,Systemic inflammation ,biology.organism_classification ,Immune system ,medicine ,medicine.symptom ,business ,Intensive care medicine - Abstract
This review presents state-of-the-art knowledge and identifies knowledge gaps for future research in the area of exercise-associated modifications of infection susceptibility. Regular moderate-intensity exercise is believed to have beneficial effects on immune health through lowering inflammation intensity and reducing susceptibility to respiratory infections. Infection-promoting consequences are attributed to strenuous exercise as performed by professional athletes. In about half of the athletes presenting respiratory symptoms, no causative pathogen can be identified. Acute bouts of exercise enhance release of proinflammatory mediators thus probably leading to appearance of infection-like respiratory symptoms. Studies assessing influence of regularly repeated exercise on immune response and systemic inflammation are far less numerous than those regarding acute exercise effects. This identifies another knowledge gap requiring further assessment both in recreational and in professional athletes Additionally, ambient and environmental conditions modify systemic inflammatory response and infection susceptibility in particular in outdoor athletes. Both acute and chronic regular exercise influence humoral and cellular immune response mechanisms resulting in decreased specific and non-specific response in competitive athletes. Most promising areas of further research in exercise immunology include: detailed immunological characterization of infection-prone and infection-resistant athletes; efficacy of nutritional and pharmaceutical interventions as countermeasures to infections’ symptoms; and influence of various exercise loads on susceptibility to infections with respiratory viruses, including SARS-CoV-2. Establishing uniform definition of “elite athlete’ shall hopefully allow for comparable and straightforward interpretation of data coming from different studies and settings.
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- 2021
45. Prevalence and early-life risk factors of school age allergic multimorbidity - the EuroPrevall-iFAAM birth cohort
- Author
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Sigridur Erla Sigurdardottir, Kristján Jónasson, Montserrat Fernandez Rivas, Paraskevi Xepapadaki, Graham Roberts, Santiago Quirce, Marek L. Kowalski, Marcin Kurowiski, Songül Yürek, Andreas Reich, Kate Grimshaw, Philip Couch, Aline B. Sprikkelman, Odilija Rudzeviciene, Nikolaos G. Papadopoulos, Lies Hulshof, Johanna Bellach, Michael Clausen, Ana Fiandor, Linus Grabenhenrich, Kirsten Beyer, Sigurveig T. Sigurdardottir, Kristin Lilja Bjornsdottir, Ruta Dubakiene, Thomas Keil, Ronald van Ree, Sina Maria Erhard, Clare Mills, Graduate School, General Paediatrics, AII - Inflammatory diseases, Ear, Nose and Throat, Experimental Immunology, APH - Global Health, APH - Personalized Medicine, and Groningen Research Institute for Asthma and COPD (GRIAC)
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Male ,0301 basic medicine ,Allergy ,Pediatrics ,CHILDREN ,Cohort Studies ,0302 clinical medicine ,Pregnancy ,Risk Factors ,Surveys and Questionnaires ,Prevalence ,Immunology and Allergy ,Prospective Studies ,MATERNAL SMOKING ,Child ,education.field_of_study ,Schools ,3. Good health ,SENSITIZATION ,Cohort ,Original Article ,Female ,eczema ,medicine.medical_specialty ,Immunology ,Population ,ECZEMA ,03 medical and health sciences ,children ,medicine ,Humans ,Medical history ,EXPOSURE ,Epidemiology and Genetics ,education ,Asthma ,allergic rhinitis ,business.industry ,Infant, Newborn ,Multimorbidity ,RHINITIS ,Odds ratio ,NATURAL-HISTORY ,asthma ,medicine.disease ,Rhinitis, Allergic ,Comorbidity ,RHINOCONJUNCTIVITIS ,030104 developmental biology ,030228 respiratory system ,allergic multimorbidity ,Observational study ,ORIGINAL ARTICLES ,business ,COMORBIDITY - Abstract
Background Coexistence of childhood asthma, eczema and allergic rhinitis is higher than can be expected by chance, suggesting a common mechanism. Data on allergic multimorbidity from a pan‐European, population‐based birth cohort study have been lacking. This study compares the prevalence and early‐life risk factors of these diseases in European primary school children. Methods In the prospective multicentre observational EuroPrevall‐iFAAM birth cohort study, we used standardized questionnaires on sociodemographics, medical history, parental allergies and lifestyle, and environmental exposures at birth, 12 and 24 months. At primary school age, parents answered ISAAC‐based questions on current asthma, rhinitis and eczema. Allergic multimorbidity was defined as the coexistence of at least two of these. Results From 10,563 children recruited at birth in 8 study centres, we included data from 5,572 children (mean age 8.2 years; 51.8% boys). Prevalence estimates were as follows: asthma, 8.1%; allergic rhinitis, 13.3%; and eczema, 12.0%. Allergic multimorbidity was seen in 7.0% of the whole cohort, ranging from 1.2% (Athens, Greece) to 10.9% (Madrid, Spain). Risk factors for allergic multimorbidity, identified with AICc, included family‐allergy‐score, odds ratio (OR) 1.50 (95% CI 1.32–1.70) per standard deviation; early‐life allergy symptoms, OR 2.72 (2.34–3.16) for each symptom; and caesarean birth, OR 1.35 (1.04–1.76). Female gender, OR 0.72 (0.58–0.90); older siblings, OR 0.79 (0.63–0.99); and day care, OR 0.81 (0.63–1.06) were protective factors. Conclusion Allergic multimorbidity should be regarded as an important chronic childhood disease in Europe. Some of the associated early‐life factors are modifiable and may be considered for prevention strategies., Allergic multimorbidity (coexistence of asthma, eczema and allergic rhinitis) is common among European children at primary school age, with 7% of study participants affected. Protective factors identified in the study include female sex, having older siblings and attending day care. Risk factors include history of allergic diseases in first‐degree family members, early‐age symptoms and caesarean birth.
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- 2021
46. Physical activity in asthma control and its immune modulatory effect in asthmatic preschoolers
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Barbara Stanic, Ya-dong Gao, Susetta Finotto, Michael Villiger, Cezmi A. Akdis, Claus Bachert, Debbie J Maurer, Mübeccel Akdis, Luo Zhang, Nikolaos G. Papadopoulos, Nan Zhang, Tuomas Jartti, Heikki Lukkarinen, Walter Kistler, Maria Pasioti, Ge Tan, Anna Graser, Chengyao Liu, Marek L. Kowalski, Paraskevi Xepapadaki, University of Zurich, and Akdis, Cezmi A
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medicine.medical_treatment ,Immunology ,Physical activity ,physical activity ,610 Medicine & health ,EXERCISE ,CHILDREN ,Peripheral blood mononuclear cell ,SPORTS ,Proinflammatory cytokine ,ACTIVATION ,chemistry.chemical_compound ,Immune system ,10183 Swiss Institute of Allergy and Asthma Research ,Asthma control ,Medicine and Health Sciences ,Immunology and Allergy ,Humans ,Medicine ,POSITION STATEMENT ,Child ,Exercise ,Asthma ,2403 Immunology ,immune modulation ,business.industry ,Zymosan ,Attendance ,Immunity ,asthma ,medicine.disease ,cytokines ,PreDicta ,ALLERGY ,Cytokine ,chemistry ,2723 Immunology and Allergy ,Leukocytes, Mononuclear ,Cytokines ,business - Abstract
Background: The impact of physical activity on immune response is a hot topic in exercise immunology, but studies involving asthmatic children are scarce. Our aims were to examine whether there were any differences in the level of physical activity and daily TV attendance, to assess its role on asthma control and immune responses to various immune stimulants. Methods: Weekly physical activity and daily television attendance were obtained from questionnaires at inclusion of the PreDicta study. PBMC cultures were stimulated with phytohemagglutinin (PHA), R848, poly I:C, and zymosan. A panel of cytokines was measured and quantified in cell culture supernatants using luminometric multiplex immunofluorescence beads-based assay. Results: Asthmatic preschoolers showed significantly more TV attendance than their healthy peers (58.6% vs. 41.5% 1–3 h daily and only 25.7% vs. 47.2% ≤1 h daily) and poor asthma control was associated with less frequent physical activity (PA) (75% no or occasional activity in uncontrolled vs. 20% in controlled asthma; 25% ≥3 times weekly vs. 62%). Asthmatics with increased PA exhibited elevated cytokine levels in response to polyclonal stimulants, suggesting a readiness of circulating immune cells for type 1, 2, and 17 cytokine release compared to subjects with low PA and high TV attendance. This may also represent a proinflammatory state in high PA asthmatic children. Low physical activity and high TV attendance were associated with a decrease in proinflammatory cytokines. Proinflammatory cytokines were correlating with each other in in vitro immune responses of asthmatic children, but not healthy controls, this correlation was more pronounced in children with sedentary behavior. Conclusion: Asthmatic children show more sedentary behavior than healthy subjects, while poor asthma control is associated with a substantial decrease in physical activity. Our results suggest that asthmatic children may profit from regular exercise, as elevated cytokine levels in stimulated conditions indicate an immune system prepared for responding strongly in case of different types of infections. However, it has to be considered that a hyperinflammatory state in high PA may not be beneficial in asthmatic children.
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- 2021
47. High-resolution allele frequencies for NGS based HLA-A, B, C, DQB1 and DRB1 typing of 23,595 bone marrow donors recruited for the Polish central potential unrelated bone marrow donor registry
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Jarosław Czerwiński, Anna Bogacz, Klaudia Nestorowicz, Bogusław Tymoniuk, Łukasz Kniżewski, Mariusz Gronkowski, Sylwia Wróblewska-Kabba, Michał Góralski, Michał Kolasiński, Alicja Bukowska, Mateusz Chraplak, Karol Jopek, Małgorzata Dudkiewicz, Mateusz Sowiński, Marek L. Kowalski, and Jacek Nowak
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0301 basic medicine ,medicine.medical_treatment ,Immunology ,Human leukocyte antigen ,Hematopoietic stem cell transplantation ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,HLA Antigens ,medicine ,Humans ,Immunology and Allergy ,Registries ,Typing ,Allele ,Allele frequency ,Bone Marrow Transplantation ,business.industry ,Histocompatibility Testing ,Haplotype ,High-Throughput Nucleotide Sequencing ,General Medicine ,Tissue Donors ,HLA-A ,Genetics, Population ,030104 developmental biology ,medicine.anatomical_structure ,Haplotypes ,Poland ,Bone marrow ,business ,030215 immunology - Abstract
Next-generation sequencing (NGS)-based typings of HLA-A, B, C, DQB1 and DRB1 loci were performed from 2018 to 2019 in 23 595 newly recruited or re-typed adult potential bone marrow donors registered in Poltransplant Registry to characterize allele and haplotype frequencies of HLA system for loci important for hematopoietic stem cell transplantation. The donors were recruited for registry and not for any other purpose including controls in a disease association study. The population sample was collected in various regions of Poland including all voivodships. The data regarding the degree of relatedness among individuals in the sample were not collected. Typings were supported by public funds as a part of the Polish National Program for Transplant Medicine Development. HLA frequency data are available in the Allele Frequencies Net Database.
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- 2020
48. Management of ocular allergy
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Jean Luc Fauquert, Dermot Ryan, Diana Silva, Banu Bozkurt, Carmen Rondon, Andrea Leonardi, Daniel Perez Formigo, Luís Delgado, Virginia L. Calder, Pia Allegri, Serge Doan, Marek L. Kowalski, Vibha Sharma, Selçuk Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, and Bozkurt, Banu
- Subjects
medicine.medical_specialty ,Eye Diseases ,Immunology ,MEDLINE ,Primary care ,Disease ,systematic review ,Risk Factors ,Hypersensitivity ,medicine ,Humans ,Immunology and Allergy ,Anti-Asthmatic Agents ,Intensive care medicine ,treatment ,business.industry ,Disease Management ,medicine.disease ,Combined Modality Therapy ,Allergic conjunctivitis ,Ocular allergy ,allergic conjunctivitis ,Calcineurin ,Treatment Outcome ,Systematic review ,ocular allergy ,management ,Disease Susceptibility ,Allergists ,business - Abstract
PubMed: 30887530, The treatment and management of ocular allergy (OA) remain a major concern for different specialties, including allergists, ophthalmologists, primary care physicians, rhinologists, pediatricians, dermatologists, clinical immunologists, and pharmacists. We performed a systematic review of all relevant publications in MEDLINE, Scopus, and Web Science including systematic reviews and meta-analysis. Publications were considered relevant if they addressed treatments, or management strategies of OA. A further wider systematic literature search was performed if no evidence or good quality evidence was found. There are effective drugs for the treatment of OA; however, there is a lack an optimal treatment for the perennial and severe forms. Topical antihistamines, mast cell stabilizers, or double-action drugs are the first choice of treatment. All of them are effective in reducing signs and symptoms of OA. The safety and optimal dosing regimen of the most effective topical anti-inflammatory drugs, corticosteroids, are still a major concern. Topical calcineurin inhibitors may be used in steroid-dependent/resistant cases of severe allergic keratoconjunctivitis. Allergen-specific immunotherapy may be considered in cases of failure of first-line treatments or to modify the natural course of OA disease. Based on the current wealth of publications and on the collective experience, recommendations on management of OA have been proposed. © 2019 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd., Funding information This work was done under the approval of EAACI with a TF budget 2015-18.
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- 2019
49. 2019 ARIA – Care pathways for allergic rhinitis – Poland
- Author
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Agnieszka Lipiec, Marek Niedoszytko, Barbara Piekarska, Peter Hellings, Dana Wallace, Filip Raciborski, Wienczyslawa Czarlewski, Piotr Kuna, Edyta Krzych-Fałta, Maciej Kupczyk, Holger J. Schünemann, Jean Bousquet, Marek L. Kowalski, Oliver Pfaar, Bolesław Samoliński, Anna Bedbrook, Marek Jutel, Ewa Jassem, and Claus Bachert
- Subjects
recommendation ,medicine.medical_specialty ,Allergy ,IMPACT ,CHILDREN ,COST-EFFECTIVENESS ,Medicine and Health Sciences ,medicine ,QUALITY ,integrated care ,General Environmental Science ,Asthma ,EAACI AIT GUIDELINES ,Science & Technology ,allergic rhinitis ,business.industry ,FLUTICASONE FUROATE ,asthma ,medicine.disease ,Integrated care ,URBAN-POPULATION ,SUBLINGUAL IMMUNOTHERAPY ,Family medicine ,General Earth and Planetary Sciences ,AZELASTINE NASAL SPRAY ,business ,Life Sciences & Biomedicine - Abstract
Integrated health care plays a pivotal role in allergic diseases. It identifies key actions (based on guidelines) in the management of allergy patients that are transferred to the local level and implemented in clinical practice. It is particularly important for allergic rhinitis to develop a new generation of guidelines for pharmacotherapy and allergen immunotherapy. Allergen-specific immunotherapy is a parallel therapy that has a preventive (or prophylactic) character and not a method indicated for those who do not respond to pharmacotherapy.
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- 2019
50. Asthma and exercise-induced respiratory disorders in athletes. The position paper of the Polish Society of Allergology and Polish Society of Sports Medicine
- Author
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Zbigniew Bartuzi, Radosław Gawlik, Andrzej Pokrywka, Hubert Krysztofiak, Marcin Kurowski, Jarosław Krzywański, Ziemowit Ziętkowski, Marek L. Kowalski, and Andrzej Bugajski
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lcsh:Internal medicine ,medicine.medical_specialty ,Allergy ,Sports medicine ,diagnosis ,Physical examination ,Dermatology ,Atopy ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Dermatology ,medicine ,Immunology and Allergy ,Medical history ,lcsh:RC31-1245 ,Intensive care medicine ,General Environmental Science ,Asthma ,treatment ,medicine.diagnostic_test ,biology ,Athletes ,business.industry ,exercise-induced bronchoconstriction ,lcsh:RL1-803 ,medicine.disease ,biology.organism_classification ,Physical therapy ,General Earth and Planetary Sciences ,Position paper ,Special Paper ,business ,human activities - Abstract
Exercise-induced respiratory symptoms describe acute airway narrowing that occurs as a result of exercise. It includes exercise-induced bronchoconstriction (EIB) and exercise-induced asthma (EIA) issues. To provide clinicians with practical guidelines, a multidisciplinary panel of stakeholders was convened to review the pathogenesis of EIB/EIA and to develop evidence-based guidelines for the diagnosis and treatment. Recommendations for the diagnosis and treatment of EIB were developed. High-intensity exercise in polluted environment (cold air, humidity, contamination, allergens) may increase the risk of EIB and asthma symptoms in athletes. Diagnostic procedures should include history taking, physical examination, atopy assessment and functional tests of the respiratory system. A strong recommendation was made for regular use of inhaled glucocorticosteroids and avoidance of short-acting β2-agonists as the only treatment. The treatment of asthma in athletes should always take into account current anti-doping regulations. This position paper reflects the currently available evidence.
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- 2019
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