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Nanoparticles have a promising future in biomedical applications and knowing whether they affect ex vivo vascular reactivity is a necessary step before their use in patients. In this study, we have evaluated the vascular effect of cerium dioxide nanoparticles (CeONPs) on the human saphenous vein in response to relaxing and contractile agonists. In addition, we have measured the protein expression of key enzymes related to vascular homeostasis and oxidative stress. We found that CeONPs increased expression of both SOD isoforms, and the consequent reduction of superoxide anion would enhance the bioavailability of NO explaining the increased vascular sensitivity to sodium nitroprusside in the presence of CeONPs. The NOX4 reduction induced by CeONPs may lead to lower HO synthesis associated with vasodilation through potassium channels explaining the lower vasodilation to bradykinin. In addition, we showed for the first time, that CeONPs increase the expression of ACE2 in human saphenous vein, and it may be the cause of the reduced contraction to angiotensin II. Moreover, we ruled out that CeONPs have effect on the protein expression of eNOS, sGC, BKca channels and angiotensin II receptors or modify the vascular response to noradrenaline, endothelin-1 and TXA analogue. In conclusion, CeONPs show antioxidant properties, and together with their vascular effect, they could be postulated as adjuvants for the treatment of cardiovascular diseases., This work was supported by Carlos III Health Institute (PI22/00424) and the European Regional Development Fund (ERDF ‘‘A way to build Europe’’ grant PI19/00838), by the Ministry of Health of the Valencian Regional Government (PROMETEO/2019/027), and by Universitat de València (UV-INV-AE-1544052).

[EN] During the 2020-2021 academic year, we designed some multimedia material to explain respiratory auscultation adapted to the first year of the Physiotherapy Degree. To assess both the subjective and objective impacts, that this material could have on our students, we collected their opinion by means of a survey and analysed the grade by means of a miniexam. The results of the surveys showed that the grade given to the multimedia material by the students was high. On the other hand, the results of the mini-exam showed that the percentage of correct answers was higher than those of errors or blank answers. Therefore, we conclude that the multimedia material has had a positive impact on self-learning. In addition, we analysed the results by groups, observing differences between them, probably due to the profile of the students, and we asked them how the material could have been improved. In conclusion, as teachers, we advocate the elaboration of these multimedia materials to support teaching, although we consider that they cannot be a substitute for classroom teaching, especially if they include a practical procedure with an important sensory part, such as respiratory auscultation., [ES] Durante el curso 2020-2021 diseñamos un material multimedia para explicar la auscultación respiratoria adaptado al nivel de primer curso de Grado de Fisioterapia. Para valorar el impacto, tanto subjetivo como objetivo, que este material podía tener en nuestro estudiantado, recogimos su opinión mediante una encuesta y analizamos la calificación mediante un mini-examen. El resultado de las encuestas mostró que la nota que el estudiantado le otorgaba al material multimedia era de notable alto. Por otra parte, los resultados del mini-examen reflejaban más porcentajes de aciertos que de errores o respuestas en blanco. Por todo ello, concluimos que el material multimedia ha tenido una repercusión positiva en el autoaprendizaje. Además, analizamos los resultados por grupos observando diferencias entre ellos, probablemente debidas al perfil del alumnado y les preguntamos cómo se podría haber mejorado el material. En conclusión, como docentes, somos partidarios de crear estos materiales multimedia como apoyo a la docencia, aunque consideramos que no pueden ser sustitutos de la presencialidad, especialmente si en ellos hay un procedimiento práctico, con una carga sensorial elevada, como es la auscultación respiratoria., PID 2020-2021: UV-SFPIE_PID20-1351987. Universitat de València. PID 2021-2022: UV-SFPIE_PID-1639310. Universitat de València.

The increase of vascular arginase activity during aging causes endothelial dysfunction. This enzyme competes with the endothelial nitric oxide synthase (eNOS) for L-arginine substrate. Our hypothesis is that glucose 6-P dehydrogenase (G6PD) overexpression could improve the endothelial function modulating the arginase pathway in aorta from mice. For this study, three groups of male mice were used: young wild type (WT) (6–9 months), old WT (21–22 months) and old G6PD-Tg (21–22 months) mice. Vascular reactivity results showed a reduced acetylcholine-dependent relaxation in the old WT but not old G6PD-Tg group. Endothelial dysfunction was reverted by nor-NOHA, an arginase inhibitor. Mice overexpressing G6PD underexpressed arginase II and also displayed a lower activity of this enzyme. Moreover, histological analyses demonstrated that age causes a thickness of aortic walls, but this did not occur in G6PD-Tg mice. We conclude that the overexpressing G6PD mouse is a model to improve vascular health via the arginase pathway.

Levamisole, a veterinary anthelmintic drug, is one of the most widely used and dangerous cocaine adulterants. Like cocaine, levamisole acutely blocks noradrenaline reuptake but with much less potency, although its vascular effects are not well known. In this study, we evaluated the vascular effects of levamisole and cocaine in rabbit aortic rings used for isometric recording of tension in organ baths and protein expression by western blot. Our results indicated that levamisole (10