Search

Leptospirosis is a zoonotic disease of global importance caused by a pathogenic group of bacteria belonging to the genus Leptospira . Here, we report four draft genome sequences of Leptospira interrogans serovar Pomona isolated on Sardinia (Italy) from four different species of mammals (i.e., dolphin, wild boar, cow, and fox).

Leptospirosis is a widespread zoonosis recognized as a re-emerging infectious disease in a wide variety of animal species, including humans and dogs. No data exist regarding the presence of Leptospira species in the canine population of Sardinia Island. This study reports the first sero-survey for leptospirosis in kennel and owned dogs from six areas of the north of Sardinia. Sera from 1296 dogs were tested by microscopic agglutination test (MAT) specific for nine different serovars that are known to be well widespread in the Mediterranean environment. Moreover, kidney homogenates from rodents collected from the study area were also analyzed by LipL32 real-time PCR and multi-locus sequence type (MLST) on the basis of the analysis of seven concatenated loci. A total of 13% of the examined sera (95%CI: 11–15) tested positive for one or more serovars of Leptospira MAT detected, antibodies for serogroup Icterohaemorrhagiae (57%, 95%CI: 49–65) were the most common, followed by serovars Bratislava (22%, 95%CI: 16–28), Canicola (14%, 95%CI: 9–19), and Grippotyphosa (7%, 95%CI: 3–11). MLST analyses on isolates from rodents identified L. interrogans and L. borgpetersenii genomospecies. Different serovars belonging to pathogenic Leptospira serogroups are circulating in dogs from the island. Moreover, data obtained from rodents, indicated that rodents likely act as reservoir of spirochetes. Further sero-epidemiological studies are needed in order to obtain data from other collection sites in Sardinia and to increase the information on Leptospira species circulating in this area.

Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the reproductive systems of wild boar hunted in two Italian regions: Tuscany and Sardinia. From 231 animals, reproductive system tissue samples (testicles, epididymides, uteri) as well as placentas and fetuses were collected. Bacteriological examination and Real-Time PCR were performed to detect pathogenic Leptospira (lipL32 gene). Leptospires were isolated from the testicles and epididymides of one adult and two subadult wild boar. Four isolates from the two subadult males were identified as Leptospira interrogans serogroup Australis by MLST, whereas Leptospira kirschneri serogroup Grippotyphosa was identified from the adult testicles and epididymis. Using Real-Time PCR, 70 samples were positive: 22 testicles (23.16%) and 22 epididymides (23.16%), 10 uteri (7.35%), 3 placentas (6.66%), and 13 fetuses (28.88%). Amplification of the rrs2 gene identified L. interrogans and L. kirschneri species. The results from this investigation confirmed that wild boar represent a potential source of pathogenic Leptospira spp. Isolation of Leptospira serogroups Australis and Grippotyphosa from the male reproductive system and the positive Real-Time PCR results from both male and female samples could suggest venereal transmission, as already demonstrated in pigs. Furthermore, placentas and fetuses were positive for the lipL32 target, and this finding may be related to a possible vertical transmission of pathogenic Leptospira., Author summary Leptospirosis is an infectious zoonotic disease present worldwide and is caused by bacteria belonging to genus Leptospira. Several domestic and wild animals could serve as reservoir hosts of these bacteria and could transmit them to humans, directly or indirectly. Generally, in asymptomatic carriers the infection involves the urinary system, the kidneys in particular, and leptospires are eliminated via urine. In some animals, Leptospira could also colonize the reproductive system as consequence of a systemic infection or venereal transmission. In this work, the Authors describe, for the first time, the detection of Leptospira from male and female reproductive organs of hunted wild boar via culture-dependent and -independent methods. These findings could suggest a possible vertical transmission in wild boar, but also a possible venereal transmission cycle among wild swine, which could accidentally involve domestic pigs too. Manipulation of these organs by hunters during slaughtering could also expose them to Leptospira infection. Obtained data add information on Leptospira epidemiology and highlight the importance of wildlife monitoring programs.

The pathogenic Leptospira species are very widespread in nature, persisting in the renal tubules of many domestic and wild animal reservoirs. We report the isolation of Leptospira interrogans serovar Pomona in a bottlenose dolphin (Tursiops truncatus) stranded along the coast of Sardinia, Italy, in 2016.