1. Production, Purification, and Properties of an Extracellular Laccase from Rigidoporus lignosus
- Author
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MariaTeresa Cambria, Santa Ragusa, Antonio Cambria, and Enrico Rizzarelli
- Subjects
Laccase ,chemistry.chemical_classification ,Sequence Homology, Amino Acid ,Chemistry ,Basidiomycota ,Molecular Sequence Data ,Culture Media ,Fungal Proteins ,Kinetics ,Enzyme ,Biochemistry ,Sequence Analysis, Protein ,Extracellular ,Substrate specificity ,Electrophoresis, Polyacrylamide Gel ,Amino Acid Sequence ,Isoelectric Focusing ,Rigidoporus lignosus ,Oxidoreductases ,Copper ,Biotechnology - Abstract
Laccase from Rigidoporus lignosus, a white-rot basidiomycete, has been isolated from culture filtrates. The enzyme was purified to homogeneity and some of its structural and kinetic parameters have been determined. The effects of pH, temperature, and organic solvents on the activity and stability of the enzyme, under different conditions, were also assayed. The results we have obtained, including the rather broad substrate specificity of enzyme, combined with their relatively easy production and purification, suggest that laccase may be efficiently employed in a variety of biotechnology applications.
- Published
- 2000
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