1. Safety and immunogenicity of myxoma virus as a new viral vector for small ruminants
- Author
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Pierre Russo, Béatrice Pignolet, Séverine Boullier, Eliane Foulon, Stéphane Bertagnoli, Jacqueline Gelfi, Gilles Meyer, Gilles Foucras, Marjorie Bozzetti, Maxence Delverdier, Agence Française de Sécurité Sanitaire des Aliments - AFSSA (FRANCE), Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Inconnu, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
- Subjects
Injections, Intradermal ,Hemorrhagic Disease Virus, Rabbit ,[SDV]Life Sciences [q-bio] ,Genetic Vectors ,Myxoma virus ,Antibodies, Viral ,Virus Replication ,Virus ,Viral vector ,03 medical and health sciences ,0302 clinical medicine ,Species Specificity ,Virology ,Animals ,Poxviridae ,030212 general & internal medicine ,Vector (molecular biology) ,Antigens, Viral ,Cells, Cultured ,ComputingMilieux_MISCELLANEOUS ,Skin ,030304 developmental biology ,Viral Structural Proteins ,Vaccines, Synthetic ,0303 health sciences ,Sheep ,Virulence ,biology ,Immunogenicity ,Vaccination ,Viral Vaccines ,Small ruminant ,Fibroblasts ,biology.organism_classification ,3. Good health ,Médecine vétérinaire et santé animal ,Chordopoxvirinae ,Viral replication ,Leukocytes, Mononuclear ,Rabbits ,Reassortant Viruses - Abstract
Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe and non-replicative vaccine vectors for other species. With the aim of developing new recombinant vaccines for ruminants, we evaluated the safety and the immunogenicity of recombinant MYXV in sheep. In vitro studies indicated that ovine primary fibroblasts were not permissive for MYXV and that infection of ovine peripheral blood mononuclear cells occurred at a low rate. Although non-specific activation significantly improved the susceptibility of lymphocytes, MYXV infection remained abortive. Histological and immunohistochemical examination at the inoculation sites revealed the development of an inflammatory process and allowed the detection of sparse infected cells in the dermis. In addition, inoculated sheep developed an antibody response directed against MYXV and the product of the transgene. Overall, these results provide the first line of evidence on the potential of MYXV as a viral vector for ruminants.
- Published
- 2008
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