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Madagascar is amongst the “hottest” biodiversity hotspots with extreme levels of diversity and endemism. Throughout the last decades, there has been substantial progress in documenting the Malagasy invertebrate fauna but no study has ever focused on pseudoscorpions (Arachnida: Pseudoscorpiones) in the arachnid fauna. Here we review the Malagasy fauna of Hercules pseudoscorpions (family Feaellidae), which are common in soil habitats of arid biomes across Madagascar. Using morphology and molecular data, we recover three reciprocally monophyletic clades that correspond to three new genera in well-defined biogeographical regions and identify twelve new species: Antsiarananaellagen. nov. for Antsirananaella lorenzorumsp. nov., Antsiarananaella leniaesp. nov., Antsiarananaella faulstichisp. nov. and Antsiarananaella marlaesp. nov.; Mahajanganellagen. nov. for Mahajanganella fridakahloaesp. nov., Mahajanganella heraclissp. nov. and Mahajanganella schwarzeneggerisp. nov.; Toliaranellagen. nov. for Toliaranella fisherisp. nov., Toliaranella griswoldisp. nov., Toliaranella mahnertisp. nov., Toliaranella meridionalissp. nov. and Toliaranella pumilasp. nov. Local endemism in this fauna is high and most species have small distributions, ranging from 20 km to 350 km linearly. Genetic distances between populations are also high, suggesting restricted dispersal or selection against dispersal in this fauna. Species’ ranges seem to be delimited by geological barriers including volcanic fields (Ambre-Bobaomby in the north of Madagascar), mountain ranges (foothills of the Central Highland Plateau), and rivers (Manankolana, Mandrare, Manombo and Onilahy Rivers and their anabranches), but mainly by different biome habitats. Overall, Madagascar emerges as a global “hotspot” of feaellid radiation and these animals may be used in future studies to test biogeographical hypotheses across xeric biomes on this island.

Previously described from only fragments of exoskeleton and juvenile specimens, the cave spider genus Troglodiplura (Araneae: Anamidae), endemic to the Nullarbor Plain, is the only troglomorphic member of the infraorder Mygalomorphae recorded from Australia. We investigated the distribution of Troglodiplura in South Australia, collecting and observing the first (intact) mature specimens, widening the number of caves it has been recorded in, and documenting threats to conservation. Phylogenetic analyses support the placement of Troglodiplura as an independent lineage within the subfamily Anaminae (the ‘Troglodiplura group’) and provide unequivocal evidence that populations from apparently isolated cave systems are conspecifics of T. beirutpakbarai Harvey & Rix, 2020, with extremely low or negligible inter-population mitochondrial divergences. This is intriguing evidence for recent or contemporary subterranean dispersal of these large, troglomorphic spiders. Observations of adults and juvenile spiders taken in the natural cave environment, and supported by observations in captivity, revealed the use of crevices within caves as shelters, but no evidence of silk use for burrow construction, contrasting with the typical burrowing behaviours seen in other Anamidae. We identify a range of threats posed to the species and to the fragile cave ecosystem, and provide recommendations for further research to better define the distribution of vulnerable taxa within caves and identify actions needed to protect them.

Australia is in the midst of an extinction crisis, having already lost 10% of terrestrial mammal fauna since European settlement and with hundreds of other species at high risk of extinction. The decline of the nation's biota is a result of an array of threatening processes; however, a comprehensive taxon‐specific understanding of threats and their relative impacts remains undocumented nationally. Using expert consultation, we compile the first complete, validated, and consistent taxon‐specific threat and impact dataset for all nationally listed threatened taxa in Australia. We confined our analysis to 1,795 terrestrial and aquatic taxa listed as threatened (Vulnerable, Endangered, or Critically Endangered) under Australian Commonwealth law. We engaged taxonomic experts to generate taxon‐specific threat and threat impact information to consistently apply the IUCN Threat Classification Scheme and Threat Impact Scoring System, as well as eight broad‐level threats and 51 subcategory threats, for all 1,795 threatened terrestrial and aquatic threatened taxa. This compilation produced 4,877 unique taxon–threat–impact combinations with the most frequently listed threats being Habitat loss, fragmentation, and degradation (n = 1,210 taxa), and Invasive species and disease (n = 966 taxa). Yet when only high‐impact threats or medium‐impact threats are considered, Invasive species and disease become the most prevalent threats. This dataset provides critical information for conservation action planning, national legislation and policy, and prioritizing investments in threatened species management and recovery., Australia is in the midst of an extinction crisis as a result of an array of threatening processes; however, a comprehensive taxon‐specific understanding of threats and their relative impacts remains undocumented nationally. Using expert consultation, we compile the first complete, validated, and consistent taxon‐specific threat and impact dataset for all 1,796 nationally listed threatened taxa in Australia. This compilation produced 4,877 unique taxon–threat combinations with the most frequently listed threats being Habitat loss, fragmentation, and degradation (n = 1,210 taxa), and Invasive species and disease (n = 966 taxa).

Long-branch attraction is a systematic artifact that results in erroneous groupings of fast-evolving taxa. The combination of short, deep internodes in tandem with long-branch attraction artifacts has produced empirically intractable parts of the Tree of Life. One such group is the arthropod subphylum Chelicerata, whose backbone phylogeny has remained unstable despite improvements in phylogenetic methods and genome-scale data sets. Pseudoscorpion placement is particularly variable across data sets and analytical frameworks, with this group either clustering with other long-branch orders or with Arachnopulmonata (scorpions and tetrapulmonates). To surmount long-branch attraction, we investigated the effect of taxonomic sampling via sequential deletion of basally branching pseudoscorpion superfamilies, as well as varying gene occupancy thresholds in supermatrices. We show that concatenated supermatrices and coalescent-based summary species tree approaches support a sister group relationship of pseudoscorpions and scorpions, when more of the basally branching taxa are sampled. Matrix completeness had demonstrably less influence on tree topology. As an external arbiter of phylogenetic placement, we leveraged the recent discovery of an ancient genome duplication in the common ancestor of Arachnopulmonata as a litmus test for competing hypotheses of pseudoscorpion relationships. We generated a high-quality developmental transcriptome and the first genome for pseudoscorpions to assess the incidence of arachnopulmonate-specific duplications (e.g., homeobox genes and miRNAs). Our results support the inclusion of pseudoscorpions in Arachnopulmonata (new definition), as the sister group of scorpions. Panscorpiones (new name) is proposed for the clade uniting Scorpiones and Pseudoscorpiones.

The pseudoscorpion (Arachnida: Pseudoscorpiones) fauna of mainland Ecuador, excluding the Galápagos Islands, is poorly known, with only 41 described species in 9 families. The family Syarinidae has a pantropical distribution and presently comprises ca 120 species in 17 valid genera that are found in leaf litter and subterranean habitats, mostly in tropical and subtropical climates. Four syarinid species have been recorded from Ecuador, including the Galápagos, in two widespread genera, Ideobisium and Ideoblothrus, but field collections suggest that these pseudoscorpions are common and abundant in all forest systems across the country. Here, we review field collections of syarinids from mainland Ecuador and describe five new species in these genera: Ideobisium kichwa sp. nov. (Napo Province, Colonso Chalupas Natural Reserve), I. sonqo sp. nov. (Napo Province, Colonso Chalupas Natural Reserve), I. susanae sp. nov. (Napo Province, Jatun Sacha Natural Reserve), Ideoblothrus nadineae sp. nov. (Napo Province, Colonso Chalupas Natural Reserve) and I. safinai sp. nov. (Pichincha Province, Otongachi Natural Reserve) based on morphology. These species seem to have narrow distributions and we therefore restrict the ranges of two species previously recorded from Ecuador (Ideobisium crassimanum Balzan, 1892 and Ideoblothrus costaricensis (Beier, 1931)) to their countries of origin, which is Costa Rica and Venezuela, respectively.

Deciphering the evolutionary relationships of Chelicerata (arachnids, horseshoe crabs, and allied taxa) has proven notoriously difficult, due to their ancient rapid radiation and the incidence of elevated evolutionary rates in several lineages. Although conflicting hypotheses prevail in morphological and molecular data sets alike, the monophyly of Arachnida is nearly universally accepted, despite historical lack of support in molecular data sets. Some phylotranscriptomic analyses have recovered arachnid monophyly, but these did not sample all living orders, whereas analyses including all orders have failed to recover Arachnida. To understand this conflict, we assembled a data set of 506 high-quality genomes and transcriptomes, sampling all living orders of Chelicerata with high occupancy and rigorous approaches to orthology inference. Our analyses consistently recovered the nested placement of horseshoe crabs within a paraphyletic Arachnida. This result was insensitive to variation in evolutionary rates of genes, complexity of the substitution models, and alternative algorithmic approaches to species tree inference. Investigation of sources of systematic bias showed that genes and sites that recover arachnid monophyly are enriched in noise and exhibit low information content. To test the impact of morphological data, we generated a 514-taxon morphological data matrix of extant and fossil Chelicerata, analyzed in tandem with the molecular matrix. Combined analyses recovered the clade Merostomata (the marine orders Xiphosura, Eurypterida, and Chasmataspidida), but merostomates appeared nested within Arachnida. Our results suggest that morphological convergence resulting from adaptations to life in terrestrial habitats has driven the historical perception of arachnid monophyly, paralleling the history of numerous other invertebrate terrestrial groups.

The name “millipede” translates to a thousand feet (from mille “thousand” and pes “foot”). However, no millipede has ever been described with more than 750 legs. We discovered a new record-setting species of millipede with 1,306 legs, Eumillipes persephone, from Western Australia. This diminutive animal (0.95 mm wide, 95.7 mm long) has 330 segments, a cone-shaped head with enormous antennae, and a beak for feeding. A distant relative of the previous record holder, Illacme plenipes from California, it belongs to a different order, the Polyzoniida. Discovered 60 m below ground in a drill hole created for mineral exploration, E. persephone possesses troglomorphic features; it lacks eyes and pigmentation, and it has a greatly elongated body—features that stand in stark contrast to its closest surface-dwelling relatives in Australia and all other members of its order. Using phylogenomics, we found that super-elongation (> 180 segments) evolved repeatedly in the millipede class Diplopoda. The striking morphological similarity between E. persephone and I. plenipes is a result of convergent evolution, probably for locomotion in similar soil habitats. Discovered in the resource-rich Goldfields-Esperance region and threatened by encroaching surface mining, documentation of this species and conservation of its habitat are of critical importance.

The modification of male pedipalps into secondary sexual intromittent organs is one of the hallmark characteristics of spiders, yet understanding the development and evolution of male genitalia across the order remains a challenging prospect. The embolus – the sclerite bearing the efferent spermatic duct or spermophor, and used to deliver sperm directly to the female genitalia during copulation – has always been considered the single unambiguously homologous palpal sclerite shared by all spider species, fundamental to the bauplan of the order and to the evolution and functional morphology of spider reproductive systems. Indeed, after two centuries of comparative research on spider reproduction, the presence of a single spermophor and embolus on each of a male spider’s two pedipalps remains a central tenet of evolutionary arachnology. Our findings challenge this premise, and reveal a remarkable twin intromittent organ sperm transfer system in a lineage of Australian palpimanoid spiders, characterized by a bifurcate spermophor and the presence of two efferent ducts leading to a pair of embolic sclerites on each pedipalp. This is the first time such a remarkable conformation has been observed in any group of arachnids with direct sperm transfer, complicating our understanding of palpal sclerite homologies, and challenging ideas about the evolution of spider genitalia.

The south-western division of Australia is the only biodiversity hotspot in Australia and is well-known for extreme levels of local endemism. Climate change has been identified as a key threat for flora and fauna, but very few data are presently available to evaluate its impact on invertebrate fauna. Here, we derive a molecular phylogeography for pseudoscorpions of the genus Pseudotyrannochthonius that in the south-west are restricted to regions with the highest rainfall. A dated molecular phylogeny derived from six gene fragments is used for biogeographic reconstruction analyses, spatial mapping, environmental niche-modelling, and to infer putative species. Phylogenetic analyses uncover nine clades with mostly allopatric distributions and often small linear ranges between 0.5 and 130 km. Molecular dating suggests that the origins of contemporary diversity fall into a period of warm/humid Palaeogene climates, but splits in the phylogeny coincide with major environmental shifts, such as significant global cooling during the Middle Miocene. By testing several models of historical biogeography available for the south-west, we determine that Pseudotyrannochthonius is an ancient relict lineage that principally follows a model of allopatric speciation in mesic zone refugia, although there are derivations from this model in that some species are older and distribution patterns more complex than expected. Ecological niche models indicate that drier and warmer future climates will lead to range contraction towards refugia of highest rainfall, probably mimicking past variations that have generated high diversity in these areas. Their conservation management will be crucial for preserving the unique biodiversity heritage of the south-west.

Acariform mites are an ancient and megadiverse lineage that may have experienced a complex pattern of invasions into terrestrial and aquatic habitats. These among-realm transitions may relate to periods of turmoil in Earth's history or be simply results of uneven biodiversity patterns across habitats. Here, we inferred a dated, representative acariform phylogeny (five genes, 9,200 bp aligned, 367 terminals belonging to 150 ingroup plus 15 outgroup families, 23 fossil calibration points) which was used to infer transitions between marine/freshwater/terrestrial habitats. We detected four unambiguous transitions from terrestrial to freshwater habitats (Hydrozetes, Naiadacarus, Fusohericia, Afronothrus, Homocaligus); one from freshwater to marine (Pontarachnidae), and four from marine to brackish or freshwater transitions (all among Halacaridae: Acarothrix; Halacarellus petiti; Copidognathus sp.; clade Limnohalacarus + Soldanellonyx + Porohalacarus + Porolohmannella). One transition to the sea was inferred ambiguously with respect to the ancestor being either terrestrial or freshwater (Hyadesiidae), and another must be most carefully examined by adding potential related taxa (Selenoribatidae + Fortuyniidae). Finally, we inferred a single, remarkable transition from aquatic to terrestrial habitats involving early evolution of the large and ecologically diverse lineage: the ancestor of the Halacaridae + Parasitengona clade was probably freshwater given our dataset, thus making terrestrial Parasitengona secondarily terrestrial. Overall, our results suggested a strong asymmetry in environmental transitions: the majority occurred from terrestrial to aquatic habitats. This asymmetry is probably linked to mites' biological properties and uneven biodiversity patterns across habitats rather than Earth's geological history. Since the land holds more acariform diversity than water habitats, a shift from the former is more likely than from the latter. We inferred the following relationships: alicid endeostigmatid + eriophyoid (Alycidae, (Nanorchestidae, (Nematalycidae, Eriophyoidea))) being sister group to the remaining Acariformes: (proteonematalycid Endeostigmata, alicorhagiid Endeostigmata, Trombidiformes, Oribatida (including Astigmata)). Trombidiform relationships had several novel rearrangements: (i) traditional Eupodina lacked support for the inclusion of Bdelloidea; (ii) Teneriffidae, traditionally placed among Anystina, was consistently recovered in a clade including Heterostigmata in Eleutherengona; (iii) several lineages, such as Adamystidae, Paratydeidae, Caeculidae and Erythracaridae, were recovered in a large clade along other Anystina and Eleutherengona, suggesting single origins of several fundamental character states, such as the reduction of the cheliceral fixed digit and development of the palpal thumb-claw complex.

Deciphering the evolutionary relationships of Chelicerata (arachnids, horseshoe crabs, and allied taxa) has proven notoriously difficult, due to their ancient rapid radiation and the incidence of elevated evolutionary rates in several lineages. While conflicting hypotheses prevail in morphological and molecular datasets alike, the monophyly of Arachnida is nearly universally accepted. Though a small number of phylotranscriptomic analyses have recovered arachnid monophyly, these did not sample all living chelicerate orders. We generated a dataset of 506 high-quality genomes and transcriptomes, sampling all living orders of Chelicerata with high occupancy and rigorous approaches to orthology inference. Our analyses consistently recovered the nested placement of horseshoe crabs within a paraphyletic Arachnida. This result was insensitive to variation in evolutionary rates of genes, complexity of the substitution models, and alternatives algorithmic approaches to species tree inference. Investigation of systematic bias showed that genes and sites that recover arachnid monophyly are enriched in noise and exhibit low information content. To test the effect of morphological data, we generated a 514-taxon morphological data matrix of extant and fossil Chelicerata, analyzed in tandem with the molecular matrix. Combined analyses recovered the clade Merostomata (the marine orders Xiphosura, Eurypterida, and Chasmataspidida), but nested within Arachnida. Our results suggest that morphological convergence resulting from adaptations to life in terrestrial habitats has driven the historical perception of arachnid monophyly, paralleling the history of numerous other invertebrate terrestrial groups.

A new genus of pseudoscorpions of the family Chernetidae, Austinochernes, is described for two new Australian species, A. andrewaustini (type species) from South Australia and Victoria, and A. zigzag from Tasmania. They are most similar to species of the Neotropical genus PseudopilanusBeier, 1957 in the possession of a spine-like seta on the prolateral face of the chelal hand at the base of the fingers, but differ by the presence of acuminate setae on the male pedipalpal trochanter. The suspected relationship between Austinochernes and Pseudopilanus suggests that their distribution is the result of Gondwanan vicariance.

The Journal of arachnology 48(3), 311 - 321 (2021). doi:10.1636/JoA-S-20-017, Pseudoscorpions are amongst the oldest terrestrial lineages but there is a major gap in the fossil record between the oldest fossils from the Devonian (ca. 385 million years ago) and rich fossil communities in amber that mostly originate from the Eocene of Europe. Burmese/Myanmar amber (or Burmite) from the middle Cretaceous preserves a diverse community of pseudoscorpions but these remain poorly documented, despite their exceptional preservation and potential to offer unique insights into evolutionary history. Here we describe a new genus and species of pseudoscorpion with a unique morphology of the chelicerae, Prionochthonius burmiticus gen. et sp. nov., from Burmese amber. Although some key characters remain obscure, the fossil can be confidently attributed to the basal pseudoscorpion family Chthoniidae, but it cannot be assigned to any extant or other fossil genus. Based on trichobothria patterns, it is most similar to extant genera that are found only in the Southern Hemisphere; findings that are in line with previous studies suggesting Gondwanan origins for at least some of the Burmese amber invertebrates. The fossil provides further evidence for a diverse community of litter- and soil-dwelling pseudoscorpions in the Late Cretaceous that comprises many extinct genera but represents many of the modern families. It also suggests that the diversification of chthoniid pseudoscorpions occurred well before the middle Cretaceous, and that the principle gestalt of its members has changed relatively little over time., Published by AAS, New York, NY

Scorpions constitute a charismatic lineage of arthropods and comprise more than 2500 described species. Found throughout various tropical and temperate habitats, these predatory arachnids have a long evolutionary history, with a fossil record that began in the Silurian. While all scorpions are venomous, the asymmetrically diverse family Buthidae harbors nearly half the diversity of extant scorpions, and all but one of the 58 species that are medically significant to humans. However, the lack of a densely sampled scorpion phylogeny has hindered broader inferences of the diversification dynamics of scorpion toxins. To redress this gap, we assembled a phylogenomic data set of 100 scorpion venom gland transcriptomes and genomes, emphasizing the sampling of highly toxic buthid genera. To infer divergence times of venom gene families, we applied a phylogenomic node dating approach for the species tree in tandem with phylostratigraphic bracketing to estimate the minimum ages of mammal-specific toxins. Our analyses establish a robustly supported phylogeny of scorpions, particularly with regard to relationships between medically significant taxa. Analysis of venom gene families shows that mammal-active sodium channel toxins (NaTx) have independently evolved in five lineages within Buthidae. Temporal windows of mammal-targeting toxin origins are correlated with the basal diversification of major scorpion mammal predators such as shrews, bats, and rodents. These results suggest an evolutionary model of relatively recent diversification of buthid NaTx homologs in response to the diversification of scorpion predators. [Adaptation; arachnids; phylogenomic dating; phylostratigraphy; venom.]

Scorpions constitute a charismatic lineage of arthropods and comprise more than 2,500 described species. Found throughout various tropical and temperate habitats, these predatory arachnids have a long evolutionary history, with a fossil record that began in the Silurian. While all scorpions are venomous, the asymmetrically diverse family Buthidae harbors nearly half the diversity of extant scorpions, and all but one of the 58 species that are medically significant to humans. Many aspects of scorpion evolutionary history are unclear, such as the relationships of the most toxic genera and their constituent venom peptides. Furthermore, the diversification age of toxins that act specifically on mammalian ion channels have never been inferred. To redress these gaps, we assembled a large-scale phylogenomic dataset of 100 scorpion venom transcriptomes and/or genomes, emphasizing the sampling of highly toxic buthid genera. To infer divergence times of venom gene families, we applied a phylogenomic node dating approach for the species tree in tandem with phylostratigraphic bracketing to estimate minimum ages of mammal-specific toxins. Our analyses establish a robustly supported phylogeny of scorpions, particularly with regard to relationships between medically significant taxa. Analysis of venom gene families shows that mammal-specific sodium channel toxins have independently evolved in five lineages within Buthidae. The temporal windows of mammal-specific toxin origins are contiguous with the basal diversification of major scorpion mammal predators such as carnivores, shrews, bats and rodents. These results suggest an evolutionary arms race model comprised of co-diversification of mammalian predators and NaTx homologs in buthid venom.

Genus-group names for animals established after 1930 must, according to the International Code of Zoological Nomenclature, be accompanied by the designation of a valid type species. We have found several genus-group names of Arachnida described after 1930 that were not validly formed and, while the type species were subsequently designated in mainstream taxonomic literature, including taxonomic revisions and catalogues, others were found to have been designated in the Zoological Record. The following names are determined to have a change of authorship: GlobochthoniusFinnegan, 1932 (not Beier, 1931), HeoblothrusBrowning, 1965 (not Beier, 1963), HesperochthoniusBrowning, 1970 (not Muchmore, 1968), TetrafeaellaMurthy & Ananthakrishnan, 1977 (not Beier, 1955) and ToxochernesFinnegan, 1934 (not Beier, 1932) (Pseudoscorpiones), RhinokarschiaHarvey, 2003 (not Birula, 1935) (Solifugae) and GinosigmaWhittick, 1937 (not Speijer, 1933) (Uropygi). Argentochernes (Pseudoscorpiones), Arenotherus, and Daesiella (Solifugae) are deemed to be nomina nuda. In addition, the type species of the following genus-group names are found to be different from conventional treatments: Chthonius (Globochthonius) histricusBeier, 1931 for Globochthonius; Chthonius (Hesperochthonius) oregonicusMuchmore, 1968 for Hesperochthonius; Feaella indica Chamberlin, 1931 for Tetrafeaella; and Allochernes (Toxochernes) similis Beier, 1932 for Toxochernes. The change in type species for Toxochernes results in its synonymy with Chernes (new synonymy), rather than with Allochernes.

The schizomid fauna of mainland Australia currently comprises 60 species within seven named genera, of which five are endemic to the continent: Attenuizomus Harvey, 2000, Brignolizomus Harvey, 2000, Draculoides Harvey, 1992, Julattenius Harvey, 1992, Notozomus Harvey, 2000. Most Australian schizomids have been described from eastern and northern Australia, but there is also a significant subterranean fauna that has been found in hypogean habitats in the semi-arid Pilbara region of Western Australia. The vast majority of these species can be assigned to the genus Draculoides and this study is the first in a proposed series to revise this highly diverse genus. We treat the species found in the western Pilbara region, which includes 13 new species and 13 previously named species, using morphological characters and multi-locus sequence data. We also incorporate a molecular “mini-barcode” approach for COI, 12S and ITS2 to diagnose the new species. The new species are named: Draculoides akashae Abrams and Harvey, n. sp., D. belalugosii Abrams and Harvey, n. sp., D. carmillae Abrams and Harvey, n. sp., D. christopherleei Abrams and Harvey, n. sp., D. claudiae Abrams and Harvey, n. sp., D. immortalis Abrams and Harvey, n. sp., D. karenbassettae Abrams and Harvey, n. sp., D. mckechnieorum Abrams and Harvey, n. sp., D. minae Abrams and Harvey, n. sp., D. noctigrassator Abrams and Harvey, n. sp., D. nosferatu Abrams and Harvey, n. sp., D. piscivultus Abrams and Harvey, n. sp. and D. warramboo Abrams and Harvey, n. sp. We also provide the first descriptions of males of D. anachoretus (Harvey, Berry, Edward and Humphreys, 2008) and D. gnophicola (Harvey, Berry, Edward and Humphreys, 2008). All of the new species are subterranean-dwelling, short-range endemic species that occur in regions subject to mining activities, rendering them of high conservation significance.

The D’Aguilar Range of subtropical south-eastern Queensland (Australia), harbours an upland rainforest biota characterised by high levels of endemic diversity. Following recent phylogenetic and biogeographic research into the open-holed trapdoor spiders of the genus Namea Raven, 1984 (family Anamidae), remarkable levels of sympatry for a single genus of mygalomorph spiders were recorded from the D’Aguilar Range. It is now known that eight different species in the genus can be found in the D’Aguilar uplands, with five apparently endemic to rainforest habitats. In this paper we present a phylogenetic and taxonomic synopsis of the remarkable anamid fauna of the D’Aguilar Range: a key to the eight species is provided, and four new species of Namea are described (N. gloriosa sp. nov., N. gowardae sp. nov., N. nebo sp. nov. and N. nigritarsus sp. nov.). In shining a spotlight on the mygalomorph spiders of this region, we highlight the D’Aguilar Range as a hotspot of subtropical rainforest diversity, and an area of considerable conservation value.

The open-holed trapdoor spider genus Aname L. Koch is widely distributed across mainland Australia and Tasmania, and currently includes 34 named species. Many species are poorly known, and their descriptions based on female type material only render their identification difficult, as the best taxonomic characters are usually found on the adult male pedipalp and first leg. To better understand the diversity of Aname in Western Australia, we present descriptions of 11 new species primarily distributed in arid ecosystems: A. baileyorum sp. nov., A. frostorum sp. nov., A. grothi sp. nov., A. lorica sp. nov., A. mcalpinei sp. nov., A. munyardae sp. nov., A. nitidimarina sp. nov., A. sinuata sp. nov., A. vernonorum sp. nov., A. watsoni sp. nov. and A. whitei sp. nov. All species are described from adult males but we were only able to confidently associate females of A. lorica, A. sinuata, A. watsoni and A. whitei. DNA sequence data were obtained for six species and were used to test morphological species hypotheses and where possible to match females and juveniles with males. Haplotype networks for A. lorica, A. sinuata and A. whitei, demonstrate that there is significant genetic structure within these species, corresponding to geographically isolated populations. We also provide the first sequence data for specimens of Aname mainaeRaven, 2000 collected from near the type locality in South Australia.

Author(s): Chamberland, L; McHugh, A; Kechejian, S; Binford, GJ; Bond, JE; Coddington, J; Dolman, G; Hamilton, CA; Harvey, MS; Kuntner, M; Agnarsson, I | Abstract: Aim: We explore the evolutionary history of the ogre-faced spiders (Deinopis) from their Early Cretaceous origins to present day. Specifically, we investigate how vicariance and dispersal have shaped distribution patterns of this lineage. Within the Caribbean, we test the role of GAARlandia, a hypothesized land bridge that connected South America to the Greater Antilles during the Eocene–Oligocene transition (~35–33nMa), in the biogeography of Deinopis. Taxon: Araneae: Deinopidae: Deinopis. Location: Caribbean islands, with additional global exemplars. Methods: Combining standard Sanger sequence data with an Anchored Hybrid Enrichment (AHE) phylogenomic dataset, we use Bayesian inference to estimate the phylogenetic relationships of Deinopis. “BioGeoBEARS” is used to test the GAARlandia hypothesis, and to pinpoint major dispersal events in the biogeographic history of Deinopis. Results: The phylogeny supports the nesting of a Caribbean clade within a continental grade. Model comparisons indicate GAARlandia as the best fitting model, and the biogeographic analyses reflect the geologic history within the Caribbean. Ancient and recent overwater dispersal events are also indicated within this lineage. There is also an ancient 113nMa split into Old and New World clades. Main Conclusions: The Deinopis phylogeny corresponds well with geography. This is reflected in the support for the GAARlandia land bridge hypothesis and the phylogenetic relationships within and among Caribbean islands mirroring nuances of Caribbean geologic history. Overwater dispersal also plays an important role in the biogeographic history of this lineage as implicated in the colonization of the volcanic and sedimentary Lesser Antilles and in a “reverse” colonization of North America. The spider family Deinopidae is an ancient lineage with origins dating back to Gondwana. While overwater dispersal has clearly played a role in the biogeography of the genus, the Deinopis phylogeny bears a strong signature of ancient geological events.

‘Slow science’ approaches to understanding the ecology, natural history and demography of species have declined over recent decades, despite the critical importance of these studies to conservation biology. With the progression of the Anthropocene, populations of invertebrates are under increasing pressure across the globe, yet few long-term datasets exist to track potential changes or declines. Here, we present a newly developed ‘slow science’ study system, to understand the demography, biology and molecular ecology of a potentially threatened species of giant idiopid trapdoor spider from inland eastern Australia. This previously undescribed species in the tribe Euoplini, here newly described as Euoplos grandis Wilson & Rix sp. nov., has a highly fragmented distribution in the southern Brigalow Belt bioregion of south-eastern Queensland, in a landscape largely cleared for cropped agriculture. The conservation significance of Idiopidae has long been recognised, and these spiders remain a flagship group for terrestrial invertebrate conservation in Australia. By studying growth rates, life spans, recruitment, natural history, fitness, gene flow, dispersal and other aspects of population and individual health, we aim gradually to uncover the population dynamics of a discrete natural population. In this paper, we summarise longitudinal data for 69 individual trapdoor spiders following an initial 18 months of study, and highlight preliminary demographic trends, biological observations and avenues for future genetic research. Ultimately, the aim of this study is to provide a baseline dataset for the conservation of Australian Idiopidae, and a guiding case study for similar taxa elsewhere in Australia.

Despite progress in recent decades, the conservation management of insects and allied invertebrates in Australia is challenging and remains a formidable task against a background of poor taxonomic and biological knowledge, limited resources (funds and scientific expertise) and a relatively low level of community engagement, education and awareness. In this review, we propose a new, strategic national approach for the conservation of insects and allied invertebrates in Australia to complement and build on existing actions and increase awareness with the general public and government. A review of all species listed under relevant State and Territory Acts, national legislation (EPBC Act) and on international lists (IUCN Red List) indicated that of the 285 species currently listed under these conservation schedules, 10 (3%) are considered extinct, 204 (72%) threatened (Critically Endangered, Endangered or Vulnerable) and 71 (25%) are classified as other (Threatened, Near Threatened, Rare or Least Concern). Comparison of the geographic ranges of listed species in relation to bioregions (IBRA regions) shows a striking discordance in spatial representation across the Australian landscape, reflecting an ad hoc approach to threatened species conservation and the concentration of invertebrate biologists in urban centres of temperate coastal Australia. There is a positive relationship between the number of threatened species and extent of protection according to the National Reserve System within each IBRA region, exemplifying the anomaly in spatial representativeness of listed species. To overcome these shortfalls, we propose a novel educational, regional approach based on selecting, for each of the 89 IBRA regions, a relatively small set of ‘flagship taxa’ (threatened species and/or ‘iconic’ species of high scientific/social value), which are then promoted and/or nominated for listing by the scientific community. Such species could be adopted by local community groups whereby a community‐based regional approach would ensure spatial representativeness of insect conservation across the entire Australian continent. This novel approach may ultimately provide a better strategy for the conservation management of habitats and threatened ecological communities, reducing extinction risk of threatened species and addressing key threatening processes. Members of the Australian entomological community are strongly encouraged to nominate candidate taxa as flagship species for wider promotion and/or listing nationally under the EPBC Act.

The scorpion family Bothriuridae occupies a subset of landmasses formerly constituting East and West temperate Gondwana, but its relationship to other scorpion families is in question. Whereas morphological data have strongly supported a sister group relationship of Bothriuridae and the superfamily Scorpionoidea, a recent phylogenomic analysis recovered a basal placement of bothriurids within Iurida, albeit sampling only a single exemplar. Here we reexamined the phylogenetic placement of the family Bothriuridae, sampling six bothriurid exemplars representing both East and West Gondwana, using transcriptomic data. Our results demonstrate that the sister group relationship of Bothriuridae to the clade (“Chactoidea” + Scorpionoidea) is supported by the inclusion of additional bothriurid taxa, and that this placement is insensitive to matrix completeness or partitioning by evolutionary rate. We also estimated divergence times within the order Scorpiones using multiple fossil calibrations, to infer whether the family Bothriuridae is sufficiently old to be characterized as a true Gondwanan lineage. We show that scorpions underwent ancient diversification between the Devonian and early Carboniferous. The age interval of the bothriurids sampled (a derived group that excludes exemplars from South Africa) spans the timing of breakup of temperate Gondwana.

The aganippine shield-backed trapdoor spiders of the monophyleticnigrum-group ofIdiosomaAusserers. l.are revised, and 15 new species are described from Western Australia and the Eyre Peninsula of South Australia:I.arenaceumRix & Harvey,sp. n.,I.corrugatumRix & Harvey,sp. n.,I.clypeatumRix & Harvey,sp. n.,I.dandaraganRix & Harvey,sp. n.,I.formosumRix & Harvey,sp. n.,I.gardneriRix & Harvey,sp. n.,I.gutharukaRix & Harvey,sp. n.,I.incomptumRix & Harvey,sp. n.,I.intermediumRix & Harvey,sp. n.,I.jarrahRix & Harvey,sp. n.,I.kopejtkaorumRix & Harvey,sp. n.,I.kwonganRix & Harvey,sp. n.,I.mcclementsorumRix & Harvey,sp. n.,I.mcnamaraiRix & Harvey,sp. n., andI.schoknechtorumRix & Harvey,sp. n.Two previously described species from south-western Western Australia,I.nigrumMain, 1952 andI.sigillatum(O. P.-Cambridge, 1870), are re-illustrated and re-diagnosed, and complementary molecular data for 14 species and seven genes are analysed with Bayesian methods. Members of thenigrum-group are of long-standing conservation significance, andI.nigrumis the only spider in Australia to be afforded threatened species status under both State and Commonwealth legislation. Two other species,I.formosumRix & Harvey,sp. n.andI.kopejtkaorumRix & Harvey,sp. n., are also formally listed as Endangered under Western Australian State legislation. Here we significantly relimitI.nigrumto include only those populations from the central and central-western Wheatbelt bioregion, and further document the known diversity and conservation status of all known species.

Pseudoscorpions have a sparse fossil record although they are among the oldest terrestrial lineages with origins that go back to the Devonian (ca. 385 Ma). Amongst the 25 extant families of pseudoscorpions, only 14 are known from fossils, most of which are preserved in European ambers from the Eocene. Burmese amber from the Cenomanian (mid-Cretaceous) of northern Myanmar is an important source of Mesozoic pseudoscorpion fossils but only six species have been described from this amber to date. In this paper, we establish the first fossil record for the pseudoscorpion family Ideoroncidae Chamberlin, 1930, which is coequally the oldest record of the pseudoscorpion superfamily Neobisioidea. The new genus Proalbiorix is established for the two species P. gracilis sp. nov. and P. compactus sp. nov. which already show all diagnostic features of members belonging to this family to date. Interestingly, Proalbiorix shows morphological features that align the fossils with present-day fauna from the Americas and Africa rather than Asia, which has biogeographical implications. Overall, the description provides another example of relative morphological stasis of pseudoscorpions compared to other arachnid lineages such as spiders, and that all major clades of pseudoscorpions were established long before the Cretaceous.

Pseudoscorpions are an ancient lineage of arachnids but have a scarce fossil record, presumably because these animals are small (2–8 mm in body length) and live in habitats where they do not fossilize easily. This is particularly the case for one of the more basal lineages, the superfamily Chthonioidea, which are common and diverse in leaf litter habitats across the world but have only been described in the fossil record from some specimens in Baltic, Dominican and Chiapas ambers. Here, we describe and illustrate the oldest pseudoscorpion of the superfamily Chthonioidea from the mid-Cretaceous (lowermost Cenomanian, ca. 99 Mya) amber of northern Myanmar and extend the known fossil record of this diverse superfamily from the Eocene into the Cretaceous. Fragments that suggest a diverse pseudoscorpion fauna have been recorded in Myanmar (Burmese) amber but this is only the fourth pseudoscorpion fossil described from this ancient amber type. The single adult female specimen is substantially older than the next oldest fossil chthonioids, which were described from Baltic amber (ca. 44–49 Mya), and displays small but significant differences to other chthonioids, such that we describe a new genus and species, Weygoldtiella plausus gen. et sp. nov. The placement of the trichobothria on the chelal hand suggests it can be attributed to the family Chthoniidae, but important morphological features are obscured in the fossil which obfuscates hypotheses regarding its nearest relatives. The specimen, although imperfectly preserved, has no known Recent relatives in this area or elsewhere in the diverse chthonioid fauna, and may represent an extinct lineage. Overall, this fossil is another example of the diverse arachnid community preserved in Burmese amber that includes lineages that are now extinct here and of considerable biogeographic interest, e.g. ricinuleids, and fauna which is essentially modern, e.g. solifuges.

Two new cave-dwelling species of the feaellid genus Cybella Judson, C. gelanggi sp. nov. and C. weygoldti sp. nov., are described from caves in Malaysia. Like other species of the genus from Vietnam and Cambodia, they possess the unusual morphology of the coxal region in which coxa III is reduced in size, and they lack well-developed plates within the pleural abdominal membrane. The first females of the genus Cybella are described.

Aim We sought to illuminate the history of the arachnid orders Schizomida and Uropygi, neither of which have previously been subjected to global molecular phylogenetic and biogeographical analyses. Location Specimens used in this study were collected in all major tropical and subtropical areas where they are presently found, including the Americas, Africa, Australia and the Indo-Pacific region. Methods From field-collected specimens, we sequenced two nuclear and two mitochondrial markers, combined these with publicly available data, and conducted multi-gene phylogenetic analyses on 240 Schizomida, 24 Uropygi and 12 other arachnid outgroups. Schizomid specimens included one specimen from the small family Protoschizomidae; other schizomid specimens were in Hubbardiidae, subfamily Hubbardiinae, which holds 289 of the order's 305 named species. We inferred ancestral areas using the Dispersal-Extinction-Cladogenesis model of range evolution, and we used fossil calibrations to estimate divergence times. Results We recovered monophyletic Schizomida and Uropygi as each other's sister group, forming the clade Thelyphonida, and terminals from the New World were usually positioned as the earliest diverging lineages. The ancestral area for schizomids reconstructed unambiguously to the region comprised of Mexico, Southern California and Florida (the xeric New World subtropics). Optimal trees suggested a single colonization of the Indo-Pacific in both orders, although this did not receive bootstrap support. Molecular dating gave an Upper Carboniferous origin for each order, and a mid-Cretaceous expansion of Schizomida, including the origin and initial diversification of those in the Indo-Pacific. Main conclusions Ancestral area reconstructions, molecular dating and fossil evidence all support an Upper Carboniferous, tropical Pangean origin for Thelyphonida, Schizomida and perhaps Uropygi. Much of this region became unsuitable habitat for these arachnids during the breakup of Pangea, but they persisted in the area that is now Meso- and South America. From there they then expanded to the Indo-Pacific, where schizomids today display an idiosyncratic combination of microendemism and long-range dispersal.

Smith, Helen M., Harvey, Mark S., Agnarsson, Ingi, Anderson, Gregory J. (2017): Notes on the Ant-mimic Genus Anatea Berland (Araneae: Theridiidae) and Two New Species from Tropical Australia. Records of the Australian Museum (Rec. Aust. Mus.) 69 (1): 1-13, DOI: 10.3853/j.2201-4349.69.2017.1672, URL: http://dx.doi.org/10.3853/j.2201-4349.69.2017.1672

The pseudoscorpion genus Haplochernes Beier, 1932, is redescribed and restricted to those species of Chernetidae with only four setae on the cheliceral hand and a pair of moderately long, slender spermathecae. This new definition is shared by only two species: the type species H. boncicus (Karsch, 1881) from Japan and possibly Taiwan and H. wuzhiensis Gao and Zhang sp. nov. from Hainan Island, China. Haplochernes madagascariensis Beier, 1932 from Madagascar and H. hagai Morikawa, 1953 from Japan are treated as new synonyms of H. boncicus.

The tropical and subtropical rainforests of Australia’s eastern mesic zone have given rise to a complex and highly diverse biota. Numerous old endemic, niche-conserved groups persist in the montane rainforests south of Cooktown, where concepts of serial allopatric speciation resulting from the formation of xeric interzones have largely driven our biogeographic understanding of the region. Among invertebrate taxa, studies on less vagile arachnid lineages now complement extensive research on vertebrate taxa, and phylogenetic studies on mygalomorph spiders in particular are revealing significant insights about the biogeographic history of the Australian continent since the Eocene. One mygalomorph lineage entirely endemic to Australia’s tropical and subtropical eastern rainforests is the open-holed trapdoor spider genus Namea Raven, 1984 (family Anamidae). We explore, for the first time, the phylogenetic diversity and systematics of this group of spiders, with the aims of understanding patterns of rainforest diversity in Namea, of exploring the relative roles of lineage overlap versus in situ speciation in driving predicted high levels of congeneric sympatry, and of broadly reconciling morphology with evolutionary history. Original and legacy sequences were obtained for three mtDNA and four nuDNA markers from 151 specimens, including 82 specimens of Namea. We recovered a monophyletic genus Namea sister to the genus Teyl Main, 1975, and monophyletic species clades corresponding to 30 morphospecies OTUs, including 22 OTUs nested within three main species-complex lineages. Remarkable levels of sympatry for a single genus of mygalomorph spiders were revealed in rainforest habitats, with upland subtropical rainforests in south-eastern Queensland often home to multiple (up to six) congeners of usually disparate phylogenetic affinity living in direct sympatry or close parapatry, likely the result of simultaneous allopatric speciation in already co-occurring lineages, and more recent dispersal in a minority of taxa. In situ speciation, in contrast, appears to have played a relatively minor role in generating sympatric diversity within rainforest ‘islands’. At the population level, changes in the shape and spination of the male first leg relative to evolutionary history reveal subtle but consistent interspecific morphological shifts in the context of otherwise intraspecific variation, and understanding this morphological variance provides a useful framework for future taxonomic monography. Based on the phylogenetic results, we further provide a detailed taxonomic synopsis of the genus Namea, formally diagnosing three main species-complexes (the brisbanensis-complex, the dahmsi-complex and the jimna-complex), re-illustrating males of all 15 described species, and providing images of live spiders and burrows where available. In doing so, we reveal a huge undescribed diversity of Namea species from tropical and subtropical rainforest habitats, and an old endemic fauna that is beginning to shed light on more complex patterns of rainforest biogeography.

The open-holed trapdoor spiders of the genus Teyl Main, 1975 from the Pilbara bioregion of Western Australia are investigated. A single endemic species from the southern Pilbara, T. heuretes sp. nov., is newly described, representing the northern-most occurrence of the genus in Australia. Legacy molecular data for Australian Nemesiidae, along with newly generated sequences for all described species of Teyl known from Western Australia, are analysed using Maximum Likelihood methods, providing molecular data for T. heuretes and an expanded phylogenetic assessment of the genus.

The goblin spider genus Cinetomorpha Simon is removed from the synonymy of Gamasomorpha Karsch and treated as the senior synonym of Yumates Chamberlin and Lucetia Dumitresco & Georgesco. All 41 species occur only in the New World, and the genus is divided into four species groups: the simplex group, the puberula group, the patquiana group and the itabaiana group. The following species are transferred to Cinetomorpha: C. floridana (Banks), comb. nov., and C. sedata (Gertsch & Mulaik), comb. nov., from Opopaea; C. angela (Chamberlin), comb. nov., and C. nesophila (Chamberlin), comb. nov., from Yumates; and C. patquiana (Birabén) and C. platensis (Birabén), comb. nov., from Gamasomorpha. Gamasomorpha wasmanniae Mello-Leitão and Lucetia distincta Dumitresco & Georgesco are newly synonymized with C. simplex Simon. Two species currently in Gamasomorpha are transferred to Hexapopha Platnick & Berniker: H. brasiliana (Bristowe), comb. nov. and H. m-scripta (Birabén), comb. nov. The following 31 species are newly described: C. adaga Ott & Bonaldo (Brazil, Colombia, Ecuador and Peru); C. atlantica Ott & Brescovit (Brazil); C. baja Ott & Ubick (Mexico); C. bandolera Ott & Harvey (Mexico & USA); C. boraceia Ott & Brescovit (Argentina and Brazil); C. campana Ott & Harvey (Chile); C. central Ott & Brescovit (Brazil); C. chicote Ott & Bonaldo (Brazil, Colombia, Ecuador and Peru); C. concepcion Ott & Harvey (Chile); C. iguazu Ott & Brescovit (Argentina and Brazil); C. itabaiana Ott & Brescovit (Brazil); C. laguna Ott & Ubick (Mexico); C. lavras Ott & Brescovit (Brazil); C. longisetosa Ott & Harvey (Costa Rica and Brazil); C. lorenzo Ott & Harvey (Guatemala); C. loreto Ott & Bonaldo (Peru); C. nayarit Ott & Harvey (Mexico); C. orellana Ott & Bonaldo (Ecuador); C. pauferro Ott & Brescovit (Brazil); C. peluda Ott & Harvey (Chile); C. pinheiral Ott & Brescovit (Brazil); C. pocone Ott & Brescovit (Brazil); C. punctata Ott & Brescovit (Brazil); C. quillota Ott & Harvey (Chile); C. rinconada Ott & Harvey (Chile); C. santamaria Ott & Brescovit (Argentina); C. similis Ott & Brescovit (Brazil); C. sternalis Ott & Bonaldo (Brazil); C. sur Ott & Ubick (Mexico); C. waorani Ott & Bonaldo (Ecuador); and C. zero Ott & Harvey (Mexico to Venezuela).

The species of the millipede genus Antichiropus Attems, 1911 found in the Pilbara region of Western Australia are reviewed, and 33 new species are described. The new species are: A. anguinus Car, n. sp., A. antius Car, n. sp., A. apricus Car, n. sp., A. cirratus Car, n. sp., A. confragus Car, n. sp., A. cristatus Car, n. sp., A. cucumeraceous Car, n. sp., A. cunicularis Car, n. sp, A. echinus Car, n. sp., A. filiolus Car, n. sp., A. forcipatus Car, n. sp., A. georginae Car, n. sp., A. gibbus Car, n. sp., A. hystricosus Car, n. sp., A. julianneae Car, n. sp., A. literulus Car, n. sp., A. lucyae Car, n. sp., A. nicholasi Car, n. sp., A. nimbus Car, n. sp., A. patriciae Car, n. sp., A. pendiculus Car, n. sp., A. picus Car, n. sp., A. procerus Car, n. sp., A. quaestionis Car, n. sp., A. rupinus Car, n. sp., A. salutus Car, n. sp., A. servulus Car, n. sp., A. simmonsi Car, n. sp., A. sloanae Car, n. sp., A. spathion Car, n. sp., A. uvulus Car, n. sp., A. verutus Car, n. sp. and A. vindicatus Car, n. sp.. The number of described Antichiropus species now stands at 72. Two species (A. julianneae Car, n. sp. and A. pendiculus Car, n. sp.) lack one diagnostic feature of the genus, namely a solenomere process, but are included here because they conform to the genus definition in all other characters. We also obtained sequence data from four mitochondrial genes (cytochrome c oxidase subunit 1 [COI], cytochrome c oxidase subunit 3 [COIII], cytochrome B [CytB], and 12S rRNA [12S]), and one nuclear gene (28S rRNA [28S]) for 19 species. Three main clades were recovered: one in the northern Pilbara, one in the southern Pilbara, and one just outside the south-western margin of the Pilbara.

Subterranean environments contain a diverse and unique obligate fauna: either aquatic living in the groundwater or terrestrial living in voids above the water table. In the arid region of the western part of the Australian continent, a particularly rich subterranean fauna coincides with a concentration of natural resource extraction operations. Since the inclusion of subterranean fauna in assessments of environmental impact in the mid-1990s, taxonomic research in Australia on this group of mainly invertebrates has grown exponentially. However, remaining knowledge gaps continue to frustrate both environmental regulators and development proponents due to high uncertainty in the decision-making process. In early 2017, the Western Australian Biodiversity Science Institute was tasked with leading the development of a research program to improve on the current state of knowledge of subterranean fauna. To balance the diverse environmental, economic and social needs of a range of stakeholders, transdisciplinary principles were applied to program development. A clear consensus on five broad focus areas to progress include: (1) data consolidation; (2) resilience to disturbance; (3) survey and sampling protocols; (4) abiotic and biotic habitat requirements; and (5) species delineation. In the context of these focus areas; we describe the research program development, reviewing the status of knowledge within each focus area, and the research initiatives to close the gaps in knowledge. We argue that, by adopting a transdisciplinary approach, the likelihood of success of the research program, as measured by the effective translation and adoption of research findings, will be maximized. This review is timely given the ever-increasing demand on groundwater systems for water extraction worldwide. A holistic understanding of the influence of anthropogenic activities on these ecosystems, and the functional role of organisms within them, will help to ensure that their health is not compromised.

The formation and spread of the Australian arid zone during the Neogene was a profoundly transformative event in the biogeographic history of Australia, resulting in extinction or range contraction in lineages adapted to mesic habitats, as well as diversification and range expansion in arid-adapted taxa (most of which evolved from mesic ancestors). However, the geographic origins of the arid zone biota are still relatively poorly understood, especially among highly diverse invertebrate lineages, many of which are themselves poorly documented at the species level. Spiny trapdoor spiders (Idiopidae: Arbanitinae) are one such lineage, having mesic ‘on-the-continent’ Gondwanan origins, while also having experienced major arid zone radiations in select clades. In this study, we present new orthologous nuclear markers for the phylogenetic inference of mygalomorph spiders, and use them to infer the phylogeny of Australasian Idiopidae with a 12-gene parallel tagged amplicon next-generation sequencing approach. We use these data to test the mode and timing of diversification of arid-adapted idiopid lineages across mainland Australia, and employ a continent-wide sampling of the fauna’s phylogenetic and geographic diversity to facilitate ancestral area inference. We further explore the evolution of phenotypic and behavioural characters associated with both arid and mesic environments, and test an ‘out of south-western Australia’ hypothesis for the origin of arid zone clades. Three lineages of Idiopidae are shown to have diversified in the arid zone during the Miocene, one (genus Euoplos) exclusively in Western Australia. Arid zone Blakistonia likely had their origins in South Australia, whereas in the most widespread genus Aganippe, a more complex scenario is evident, with likely range expansion from southern Western Australia to southern South Australia, from where the bulk of the arid zone fauna then originated. In Aganippe, remarkable adaptations to phragmotic burrow-plugging in transitional arid zone taxa have evolved twice independently in Western Australia, while in Misgolas and Cataxia, burrow door-building behaviours have likely been independently lost at least three times in the eastern Australian mesic zone. We also show that the presence of idiopids in New Zealand (Cantuaria) is likely to be the result of recent dispersal from Australia, rather than ancient continental vicariance. By providing the first comprehensive, continental synopsis of arid zone biogeography in an Australian arachnid lineage, we show that the diversification of arbanitine Idiopidae was intimately associated with climate shifts during the Neogene, resulting in multiple Mio-Pliocene radiations.

Michael G Rix, Joel A Huey, Barbara Y Main, Julianne M Waldock, Sophie E Harrison, Sarah Comer, Andrew D Austin and Mark S Harvey

A review of the pseudoscorpion family Ideoroncidae in the Asian region reveals three genera: Dhanus Chamberlin, 1930, Shravana Chamberlin, 1930 and a new genus. Dhanus includes four species, D. sumatranus (Redikorzev, 1922), D. hashimi sp. nov. and D. tioman sp. nov. from Malaysia, and D. lunaris sp. nov. from Cambodia. Dhanus doveri Bristowe, 1952 is newly synonymized with D. sumatranus, and the type locality of D. sumatranus is confirmed as the Dark Cave, in the Batu Cave system, near Kuala Lumpur. Shravana is confirmed as a senior synonym of Nhatrangia Redikorzev, 1938 and includes 13 species: S. laminata (With, 1906) and S. schwendingeri sp. nov., from Thailand, S. charas sp. nov. and S. withi sp. nov. from Malaysia, S. dawydoffi (Redikorzev, 1938), comb. nov. (transferred from Nhatrangia) from Cambodia, Laos, Vietnam and the Spratly Islands, S. indica (Murthy & Ananthakrishnan, 1977), comb. nov. (transferred from Dhanus) from India, S. ceylonensis (Mahnert, 1984), comb. nov. (transferred fro...