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24 results on '"Maroto, Aroa S."'

10. Erratum to: KLK1 and ZG16B proteins and arginine–proline metabolism identified as novel targets to monitor atherosclerosis, acute coronary syndrome and recovery

Author

Martin-Lorenzo, Marta, Zubiri, Irene, Maroto, Aroa S., Gonzalez-Calero, Laura, Posada-Ayala, Maria, de la Cuesta, Fernando, Mourino-Alvarez, Laura, Lopez-Almodovar, Luis F., Calvo-Bonacho, Eva, Ruilope, Luis M., Padial, Luis R., Barderas, Maria G., Vivanco, Fernando, and Alvarez-Llamas, Gloria

Published
2016
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12. Corrigendum to “Novel liquid chromatography–mass spectrometry method for sensitive determination of the mustard allergen Sin a 1 in food” [Food Chem. 183 (2015) 58–63]

Author

Posada-Ayala, Maria, primary, Alvarez-Llamas, Gloria, additional, Maroto, Aroa S., additional, Maes, Xavier, additional, Muñoz-Garcia, Esther, additional, Villalba, Mayte, additional, Rodríguez, Rosalía, additional, Perez-Gordo, Marina, additional, Vivanco, Fernando, additional, Pastor-Vargas, Carlos, additional, and Cuesta-Herranz, Javier, additional

Published
2016
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14. Cytoskeleton deregulation and impairment in amino acids and energy metabolism in early atherosclerosis at aortic tissue with reflection in plasma

Author

Martin-Lorenzo, Marta, primary, Gonzalez-Calero, Laura, additional, Maroto, Aroa S., additional, Martinez, Paula J., additional, Zubiri, Irene, additional, de la Cuesta, Fernando, additional, Mourino-Alvarez, Laura, additional, Barderas, Maria G., additional, Heredero, Angeles, additional, Aldamiz-Echevarría, Gonzalo, additional, Vivanco, Fernando, additional, and Alvarez-Llamas, Gloria, additional

Published
2016
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16. Urinary alpha-1 antitrypsin and CD59 glycoprotein predict albuminuria development in hypertensive patients under chronic renin-angiotensin system suppression

Author

Gonzalez-Calero, Laura, primary, Martin-Lorenzo, Marta, additional, de la Cuesta, Fernando, additional, Maroto, Aroa S., additional, Baldan-Martin, Montserrat, additional, Ruiz-Hurtado, Gema, additional, Pulido-Olmo, Helena, additional, Segura, Julian, additional, Barderas, Maria G., additional, Ruilope, Luis M., additional, Vivanco, Fernando, additional, and Alvarez-Llamas, Gloria, additional

Published
2016
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17. KLK1 and ZG16B proteins and arginine–proline metabolism identified as novel targets to monitor atherosclerosis, acute coronary syndrome and recovery

Author

Martin-Lorenzo, Marta, primary, Zubiri, Irene, additional, Maroto, Aroa S., additional, Gonzalez-Calero, Laura, additional, Posada-Ayala, Maria, additional, de la Cuesta, Fernando, additional, Mourino-Alvarez, Laura, additional, Lopez-Almodovar, Luis F., additional, Calvo-Bonacho, Eva, additional, Ruilope, Luis M., additional, Padial, Luis R., additional, Barderas, Maria G., additional, Vivanco, Fernando, additional, and Alvarez-Llamas, Gloria, additional

Published
2014
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22. Laser microdissection and saturation labeling DIGE method for the analysis of human arteries.

Author

de la Cuesta F, Alvarez-Llamas G, Maroto AS, Barderas MG, and Vivanco F

Subjects
Apoptosis, Arteries physiopathology, Blood Coagulation, Coronary Artery Disease metabolism, Coronary Artery Disease pathology, Coronary Artery Disease physiopathology, Extracellular Matrix metabolism, Heat-Shock Response, Hemorrhage metabolism, Hemorrhage pathology, Humans, Staining and Labeling, Tandem Mass Spectrometry, Tunica Intima metabolism, Tunica Intima pathology, Tunica Intima physiopathology, Tunica Media metabolism, Tunica Media pathology, Tunica Media physiopathology, Arteries metabolism, Arteries pathology, Laser Capture Microdissection methods, Two-Dimensional Difference Gel Electrophoresis methods
Abstract

Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.

Published
2013
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23. Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS.

Author

de la Cuesta F, Barderas MG, Calvo E, Zubiri I, Maroto AS, Lopez JA, Vivanco F, and Alvarez-Llamas G

Subjects
Breast blood supply, Chromatography, Liquid, Coronary Vessels metabolism, Electrophoresis, Gel, Two-Dimensional, Humans, Peptide Fragments isolation & purification, Peptide Fragments metabolism, Proteolysis, Arteries cytology, Arteries metabolism, Nanotechnology, Proteomics methods, Tandem Mass Spectrometry
Abstract

Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.

Published
2013
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24. An optimum method designed for 2-D DIGE analysis of human arterial intima and media layers isolated by laser microdissection.

Author

de la Cuesta F, Alvarez-Llamas G, Maroto AS, Donado A, Juarez-Tosina R, Rodriguez-Padial L, Pinto AG, Barderas MG, and Vivanco F

Abstract

The formation and progression of atherosclerotic lesions involve complex mechanisms which are still not fully understood. A variety of cell types from the distinct arterial layers are implicated in the whole process from lipid accumulation within the vascular wall to plaque development and final rupture. In the present work, we employ the combination of laser microdissection and pressure catapulting and 2-D DIGE saturation labeling to investigate the human intima and media sub-proteomes isolated from atherosclerotic (coronary and aorta) or non-atherosclerotic vessels (preatherosclerotic coronary arteries). Laser microdissection and pressure catapulting allows the specific isolation of regions of interest. In turn, DIGE saturation labeling overcomes the limitation of extensive microdissection times to recover the protein amount required to perform comparative 2-DE, particularly when dealing with tissue regions rich in myofilament proteins, which result in low protein recovery. The compatibility and optimum performance of both techniques were investigated in detail, paying special attention to tissue staining and protein solubilization. Since scarce amount of protein obtained from microdissected tissue made it impossible to directly perform protein identification from 2-DE spots by MS, we performed in-solution digestion followed by LC-MS/MS analysis of total protein extracts from intima and media in order to get an overall picture of protein composition. Proteins so identified confirm the nature of the isolated regions. Finally, similar spot resolution on 2-D DIGE gels was obtained for the different human artery types (coronary, aorta) and studied layers (intima, media), setting the basis for future clinical comparative studies., (Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2009
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