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This study presents a simulation of an organic Rankine cycle (ORC) for the utilization of combustion gas produced in small landfills in Antioquia, Colombia, with a focus on the municipality of Angostura. This municipality has been chosen as the focus of this study due to its growing population and industrial and tourism development, bringing with them the need for sustainable waste and energy management solutions. The proposed ORC system includes two evaporators, two turbines, a condenser, a pump, and a generator, similar to successful systems reported in the literature. A sensitivity analysis was performed to investigate the impact of pressure, temperature, and mass flow on the system's net power output and thermodynamic efficiency. The results showed that the system reached a net power output of 64.33   kW with an overall power plant efficiency of 13.03 % and an investment cost of 192,340   USD based on a reference cost of 2990   USD / kW . The study also found that the system's use in Angostura is economically feasible, with a net present value of 31,208   USD over a 10-year investment study. The sensitivity analysis revealed that temperature and pressure have direct effects on the system's performance and economic viability. The municipality's geomorphological characteristics were found to reduce the risk of groundwater contamination, while surface waters may still be vulnerable to contamination from leachates. Overall, this study highlights the feasibility and potential benefits of utilizing an ORC system, in which the combustion heat from methane gas produced in small landfills in Antioquia, Colombia, is harnessed for energy production. [ABSTRACT FROM AUTHOR]

Reovirus is known to have an anticancer effect in both the preclinical and clinical assays. Current evidence suggests that the reovirus-mediated impact on tumor growth depends on the activation of specific antitumor immune responses. A feasible explanation for the oncolytic effects and immune system activation is through the expression of the fusogenic reovirus protein. In this work, we evaluated the in vivo antitumor effects of the expression of fusogenic protein p10 of avian reovirus (ARV-p10). We used chitosan nanoparticles (CH-NPs) as a vehicle for the ARV-p10 DNA in murine B16 melanoma models both in vitro and in vivo. We confirmed that ARV-p10 delivery through a chitosan-based formulation (ARV-p10 CH-NPs) was capable of inducing cell fusion in cultured melanoma cells, showing a mild cytotoxic effect. Interestingly, intratumor injection of ARV-p10 CH-NPs delayed tumor growth, without changing lymphoid populations in the tumor tissue and spleen. The injection of chitosan nanoparticles (CH-NPs) also delayed tumor growth, suggesting the nanoparticle itself would attack tumor cells. In conclusion, we proved that in vitro ARV-p10 protein expression using CH-NPs in murine melanoma cells induces a cytotoxic effect associated with its cell fusion. Further studies are necessary for establishing a protocol for efficient in vivo DNA delivery of fusion proteins to produce an antitumoral effect. [ABSTRACT FROM AUTHOR]

Reovirus is known to have an anticancer effect in both the preclinical and clinical assays. Current evidence suggests that the reovirus-mediated impact on tumor growth depends on the activation of specific antitumor immune responses. A feasible explanation for the oncolytic effects and immune system activation is through the expression of the fusogenic reovirus protein. In this work, we evaluated the in vivo antitumor effects of the expression of fusogenic protein p10 of avian reovirus (ARV-p10). We used chitosan nanoparticles (CH-NPs) as a vehicle for the ARV-p10 DNA in murine B16 melanoma models both in vitro and in vivo. We confirmed that ARV-p10 delivery through a chitosan-based formulation (ARV-p10 CH-NPs) was capable of inducing cell fusion in cultured melanoma cells, showing a mild cytotoxic effect. Interestingly, intratumor injection of ARV-p10 CH-NPs delayed tumor growth, without changing lymphoid populations in the tumor tissue and spleen. The injection of chitosan nanoparticles (CH-NPs) also delayed tumor growth, suggesting the nanoparticle itself would attack tumor cells. In conclusion, we proved that in vitro ARV-p10 protein expression using CH-NPs in murine melanoma cells induces a cytotoxic effect associated with its cell fusion. Further studies are necessary for establishing a protocol for efficient in vivo DNA delivery of fusion proteins to produce an antitumoral effect. [ABSTRACT FROM AUTHOR]