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1. Lösliche Biofilmfaktoren von Staphylococcus aureus und Staphylococcus epidermidis beeinflussen osteogene Prozesse in primären humanen Osteoblasten patientenabhängig

Author

Tübel, J, Jegen, M, Maier, E, Marthen, C, Obermeier, A, von Eisenhart-Rothe, R, and Burgkart, R

Subjects
ddc: 610, Medicine and health
Abstract

Fragestellung: Hauptverursacher für implantatassoziierte Infektionen sind die Biofilmformer Staphylococcus aureus (SA) und Staphylococcus epidermidis (SE). Die unterschiedliche Pathogenität der Biofilme wird im Wesentlichen durch die keimspezifische Sekretion zahlreicher löslicher Faktoren [zum vollständigen Text gelangen Sie über die oben angegebene URL]

Published
2021
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2. Einfluss von biofilm-konditioniertem Kulturmedium von Staphylococcus aureus und Staphylococcus epidermidis auf inflammatorische Prozesse in primären humanen Osteoblasten

Author

Tübel, J, Jegen, M, Maier, E, Marthen, C, Obermeier, A, Ostermeier, M, von Eisenhart-Rothe, R, and Burgkart, R

Subjects
Inflammation, ddc: 610, Osteoblasten, Implantat-assoziierte Infektionen, 610 Medical sciences, Medicine, S. aureus, S. epidermidis
Abstract

Fragestellung: Implantat-assoziierte Infektionen sind gekennzeichnet durch eine überwiegende Besiedelung von Staphylococcus aureus (SA) (33%) und Staphylococcus epidermidis (SE) (31%). Beide Biofilmbildner überziehen die Implantatoberfläche mit Biofilm, der dann aufgrund der[zum vollständigen Text gelangen Sie über die oben angegebene URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2019)

Published
2019
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4. LUPUS VULGARIS DENGAN LESI DISEMINATA

Author

Niken Ernaningtyas, Nana N. Jayadi, Nurdjannah J. Niode, and Marthen C. P. Wongkar

Abstract

Lupus vulgaris (LV) is a chronic progressive form of paucibacillary cutaneous tuberculosis. Lesion is usually solitary in the form of nodes or erythematous plaques with an apple-jelly sign on diascopy. Disseminated LV is a rare form of cutanoeus tuberculosis with multiple lesions in several body areas. We reported a male of 40 years old with a suppurative wound on the left neck and reddish nodules on the face, neck, trunk, and limbs along with fever, night sweats, weight loss, and history of previous TB infections. There were multiple erythematous nodules and painful suppurating ulcers with enlargement of several lymph nodes. Apple-jelly sign appeared on diascopy. The FNAB showed specific granulamatous inflammation for TB with lymphocytes, epitheloid macrophages, and multinucleated giant cells. The histopathological finding showed tubercles surrounded by macrophages and lymphocytes. Anti-tuberculosis drugs category I were given for 6 months, ofloxacin, and open wound care compressed with NaCl 0.9%. In the third month of observation, there was significant improvement. Conclusion: This case was diagnosed as lupus vulgaris based on the history of lymphadenitis TB and scrofuloderma, lesions in several body area with positive diascopy test, the FNAB as well as the histopathologic result supporting the diagnosis of tuberculosis, and there was significant improvement after treatment with antiTB drugs.Keywords: lupus vulgaris, diseminata, tuberculosis, ofloksasinAbstrak: Lupus vulgaris (LV) merupakan tuberkulosis (TB) kutis pausibasiler kronis dan progresif. Lesi biasanya soliter, berupa nodus atau plak eritematosa dengan gambaran apple-jelly pada diaskopi. Lupus vulgaris diseminata merupakan bentuk TB kutis yang jarang ditemukan dengan lesi multipel pada beberapa area tubuh secara bersamaan. Kami melaporkan seorang laki-laki, 40 tahun, dengan luka bernanah pada leher kiri dan benjolan-benjolan kemerahan pada wajah, leher, badan, dan tungkai disertai demam, keringat malam, penurunan berat badan dan riwayat infeksi tuberkulosis sebelumnya. Pada pemeriksaan fisik tampak nodus eritematosa multipel disertai ulkus bernanah dan pembesaran beberapa kelenjar getah bening. Gambaran apple-jelly tampak pada diaskopi. Pemeriksaan FNAB menunjukkan gambaran radang granulomatik spesifik TB dengan adanya sel-sel radang limfosit, kelompok makrofag epiteloid, dan sel-sel datia Langhans. Pemeriksaan histopatologis memberikan gambaran tuberkel yang dikelilingi oleh makrofag dan limfosit. Terapi diberikan berupa OAT kategori I selama 6 bulan, ofloksasin, dan kompres terbuka dengan NaCL 0,9%. Pada bulan ketiga tampak perbaikan signifikan. Simpulan: Pada kasus ini, diagnosis lupus vulgaris ditegakkan berdasarkan adanya riwayat limfadenitis TB dan skrofuloderma, lesi di beberapa area tubuh sekaligus dengan pemeriksaan diaskopi positif, gambaran FNAB dan histopatologis menunjang diagnosis TB, dan pengobatan dengan OAT memberikan perbaikan bermakna.Kata kunci: lupus vulgaris, diseminata, OAT, ofloksasin

Published
2015

5. Evaluierung der Temperaturverteilung an einem Miniaturinkubator für das Tissue Engineering

Author

Föhr, P, Quixtner, S, Tübel, J, Kuntz, L, Marthen, C, von Eisenhart-Rothe, R, and Burgkart, R

Subjects
ddc: 610, Tissue Engineering, Zellkultur, Osteoblasten, Temperaturverteilung, 610 Medical sciences, Medicine, Bioreaktor, Inkubator
Abstract

Fragestellung: Für die 3D-Gewebezüchtung sind oftmals Eigenkonstruktionen nötig, um neue Ansätze evaluieren zur können. Neben der Verwendung geeigneter Werkstoffe oder der Sicherstellung der Sterilität ist auch ein homogener, nicht schwankender Temperaturverlauf von entscheidendem[zum vollständigen Text gelangen Sie über die oben angegebene URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2015)

Published
2015
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6. Estradiol beeinflusst den Methylierungsstatus des ER alpha Promotors und die Expression knochenspezifischer Gene in Osteosarkomzellen SAOS-2

Author

Tübel, J, Kuntz, L, Marthen, C, Wiest, I, von Eisenhart-Rothe, R, Jeschke, U, and Burgkart, R

Subjects
Epigenetik, ddc: 610, Estradiol, Knochenstoffwechsel, Estrogenrezeptor alpha, Osteosarkom, 610 Medical sciences, Medicine
Abstract

Fragestellung: Die Bedeutung des Steroidhormons Estradiol (E2) auf den Knochenstoffwechsel und Knochentumore ist bekannt. Durch Bindung des Liganden Estradiol an den Estrogenrezeptor alpha (ER α) wird dieser aktiviert, bindet an das "estrogen response element" (ERE) und steuert als[zum vollständigen Text gelangen Sie über die oben angegebene URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2015)

Published
2015
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9. Pilotstudie zur Evaluierung des Einflusses des Kulturmodells – 3D vs. 2D – auf die osteogene Differenzierung von primären humanen Osteoblasten

Author

Kuntz, L, Tübel, J, Marthen, C, Hilz, F, von Eisenhart-Rothe, R, and Burgkart, R

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Fragestellung: Trotz der hohen Regenerationsfähigkeit von Knochen werden bei kritischen Knochendefekten häufig Knochentransplantationen benötigt. Knochen Tissue Engineering hat das Potenzial, zu maßgeblichen Verbesserungen in der Entwicklung neuer Knochentransplantate beizutragen,[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2014)

Published
2014
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10. Beeinflusst die Menopause die Stimulierbarkeit des Vitamin D Rezeptors in primären humanen Osteoblasten?

Author

Tübel, J, Marthen, C, Kuntz, L, Hilz, F, von Eisenhart-Rothe, R, and Burgkart, R

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Fragestellung: Krankheiten des Knochens, wie z. B. die Osteoporose gewinnen durch den ständigen Anstieg der Lebenserwartung immer mehr an Bedeutung. Der Einfluss der Hormone auf den Knochenstoffwechsel ist seit langer Zeit bekannt. So steuert z.B. Estradiol (E2) u.a. die Proliferation und Differenzierung[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2014)

Published
2014
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11. Das Expressionsprofil von Adhäsionsproteinen primärer humaner Osteoblasten nach 20-wöchiger Langzeitkultur in 2D versus 3D Kulturmodellen

Author

Tübel, J, Marthen, C, Saldamli, B, Dorfner, P, Tron, A, von Eisenhart-Rothe, R, and Burgkart, R

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Fragestellung: Adhäsionsproteine mit ihren unterschiedlichen Funktionen, wie z. B. Fokal- oder Zell/Zell-Adhäsion spielen im gesamten Bereich des Tissue Engineering und der damit verbundenen dreidimensionalen Zellkultur eine ebenso wichtige Rolle wie bei der erfolgreichen ossären[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2012)

Published
2012
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14. Untersuchung der Expression von Progesteronrezeptor A und B und Estrogenrezeptor α und β in humanen Osteosarkomzellen

Author

Tübel, J, Saldamli, B, Marthen, C, Burgkart, R, Jeschke, U, and Gradinger, R

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Fragestellung: Die Steroidhormone Estradiol (E) und Progesteron (P) sind bekannt für ihre Wirkungen auf den Knochenstoffwechsel. Diese Hormone können die Zielzellen durch Bindung und Aktivierung an den Estrogenrezeptor (ER) bzw. Progesteronrezeptor (PR) regulieren. ER und PR gehören [for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie; 74. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 96. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 51. Tagung des Berufsverbandes der Fachärzte für Orthopädie

Published
2010
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20. Patient-specific effects of soluble factors from Staphylococcus aureus and Staphylococcus epidermidis biofilms on osteogenic differentiation of primary human osteoblasts.

Author

Tübel J, Maier E, Jegen M, Marthen C, Obermeier A, Haug AT, Schneider J, and Burgkart R

Subjects
Adult, Aged, Biofilms drug effects, Cell Differentiation drug effects, Cells, Cultured, Culture Media, Conditioned metabolism, Culture Media, Conditioned pharmacology, Female, Gene Expression drug effects, Humans, Male, Middle Aged, Osteoblasts cytology, Osteoblasts metabolism, Osteogenesis physiology, Species Specificity, Staphylococcal Infections genetics, Staphylococcus aureus metabolism, Staphylococcus aureus pathogenicity, Staphylococcus epidermidis metabolism, Staphylococcus epidermidis pathogenicity, Virulence, Biofilms growth & development, Cell Differentiation physiology, Osteoblasts microbiology, Staphylococcal Infections microbiology, Staphylococcus aureus physiology, Staphylococcus epidermidis physiology
Abstract

Due to the frequency of biofilm-forming Staphylococcus aureus and Staphylococcus epidermidis in orthopedics, it is crucial to understand the interaction between the soluble factors produced by prokaryotes and their effects on eukaryotes. Our knowledge concerning the effect of soluble biofilm factors (SBF) and their virulence potential on osteogenic differentiation is limited to few studies, particularly when there is no direct contact between prokaryotic and eukaryotic cells. SBF were produced by incubating biofilm from S. aureus and S. epidermidis in osteogenic media. Osteoblasts of seven donors were included in this study. Our results demonstrate that the detrimental effects of these pathogens do not require direct contact between prokaryotic and eukaryotic cells. SBF produced by S. aureus and S. epidermidis affect the metabolic activity of osteoblasts. However, the effect of SBF derived from S. aureus seems to be more pronounced compared to that of S. epidermidis. The influence of SBF of S. aureus and S. epidermidis on gene expression of COL1A1, ALPL, BGLAP, SPP1, RUNX2 is bacteria-, patient-, concentration-, and incubation time dependent. Mineralization was monitored by staining the calcium and phosphate deposition and revealed that the SBF of S. epidermidis markedly inhibits calcium deposition; however, S. aureus shows a less inhibitory effect. Therefore, these new findings support the hypotheses that soluble biofilm factors affect the osteogenic processes substantially, particularly when there is no direct interaction between bacteria and osteoblast., (© 2021. The Author(s).)

Published
2021
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21. Methylation of the ER-alpha Promoter Is Influenced by its Ligand Estrogen in Osteosarcoma Cells SAOS-2 In Vitro.

Author

Tübel J, Kuntz L, Marthen C, Schmitt A, Wiest I, VON Eisenhart-Rothe R, Jeschke U, and Burgkart R

Subjects
Cell Line, Tumor, Estrogen Receptor alpha analysis, Humans, Ligands, RNA, Messenger analysis, Real-Time Polymerase Chain Reaction, Bone Neoplasms genetics, DNA Methylation drug effects, Estrogen Receptor alpha genetics, Estrogens pharmacology, Osteosarcoma genetics, Promoter Regions, Genetic
Abstract

Background/aim: The aggressive fast-growing osteosarcoma is the most common primary malignant bone tumor. The relevance of estrogen as a key player in bone metabolism and bone tumor is well-known. At the molecular level, estrogen activates the estrogen receptor α (ERα) as a natural ligand of this receptor. ERα acts as a transcription factor by binding to the "estrogen response element" (ERE) and regulates the expression of a various number of genes. Epigenetic processes, e.g. the methylation of the "cytosine-phosphatidyl-guanine (CpG) islands" can change the transcription of target genes and subsequently the protein expression. As DNA methylation is generally associated with gene transcription repression, up until now little is known about the ERα methylation in osteosarcoma cells. The aim of the present pilot study was to evaluate the methylation status of ERα in osteosarcoma cells SAOS-2 and MG 63 after stimulation with estrogen., Materials and Methods: SAOS-2 and MG 63 cells were cultured in DMEM. After treatment with 10 nmol estrogen (E2) for 24 h, the expression of ERα was detected by immunocytochemistry (ICC). As controls we used untreated cells. Staining was evaluated semi-quantitatively by the immunoreactive score of Remmele and Stegner (IRS). To determine mRNA gene expression, extracted RNA was transcribed into c-DNA and a quantitative real-time-PCR (qRT-PCR) was carried out. The semi quantitative evaluation of the ERα mRNA was based on the 2(-ΔΔct) method using untreated cells as reference control. One microgram of each extracted genomic DNA sample was converted with bisulfite and a real-time methylation-specific PCR (rt-MSP) was performed., Results: The estrogen-stimulated SAOS-2 cells showed a significant increase of ERα expression. A 7-fold up-regulation of ERα mRNA confirmed the results of immunocytochemistry. Methylation of the ERα promoter was not detected in treated cells. In contrast, we identified methylation of the ERα promoters in untreated cells. The staining of MG 63 cells showed a weak gain of ERα expression in the stimulated cells, as well as a weak increase of the ER-α mRNA (2-fold). Methylation of the ERα promoters was not detectable in either treated or untreated cells., Conclusion: The methylation status of ERα in osteosarcoma cells is affected by estrogen. These findings indicate that epigenetic changes of genomic DNA regulate ERα synthesis. Taken together, our results suggest that SAOS-2 cells can be an interesting model for further investigating ERα synthesis. In addition, the evaluation of ERα methylation in osteosarcoma specimens is in progress., (Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)

Published
2016

22. Expression of osteopontin, a target gene of de-regulated Wnt signaling, predicts survival in colon cancer.

Author

Rohde F, Rimkus C, Friederichs J, Rosenberg R, Marthen C, Doll D, Holzmann B, Siewert JR, and Janssen KP

Subjects
Adenoma chemistry, Adenoma mortality, Aged, Animals, Colon chemistry, Colonic Neoplasms genetics, Female, Gene Expression Regulation, Neoplastic, Genes, APC, Humans, Liver Neoplasms chemistry, Liver Neoplasms mortality, Liver Neoplasms secondary, Male, Mice, Middle Aged, Mutation, Precancerous Conditions chemistry, Precancerous Conditions mortality, Predictive Value of Tests, Prognosis, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Survival Analysis, Up-Regulation, beta Catenin analysis, beta Catenin genetics, Biomarkers, Tumor analysis, Biomarkers, Tumor genetics, Colonic Neoplasms chemistry, Colonic Neoplasms mortality, Osteopontin analysis, Osteopontin genetics, Wnt Proteins genetics
Abstract

Osteopontin (OPN) is a secreted phosphoprotein, which has been reported to be associated with tumor progression in numerous solid tumors. In a previous transcriptome study on colorectal cancer, we identified the gene OPN among the most strongly up-regulated transcripts. OPN has been suggested as a putative target of Wnt signaling, but the molecular mechanism responsible for its aberrant transcription is not fully understood. We analyzed 13 normal colon tissues, 9 adenomas, 120 primary colon tumors, and 10 liver metastases by quantitative reverse-transcription PCR. OPN expression was strongly elevated in primary colon cancer and liver metastasis, but not in pre-cancerous lesions and UICC stage I tumors. Multivariate analysis established OPN expression as an independent prognostic parameter for overall survival. Moreover, high OPN expression identified a subgroup of patients with bad prognosis. Next, we determined immunohistochemically a correlation of OPN expression with aberrant beta-catenin staining, which is indicative of Wnt activation. Elevated expression of OPN was significantly correlated with increased cytoplasmic and nuclear beta-catenin staining. The in vivo role of Wnt signaling for the expression of OPN was tested in genetically defined mouse models with (Apc(1638N)) or without (pvillin-KRAS(V12G)) Wnt activating mutations. Mutation of the tumor suppressor APC was necessary for upregulation of OPN expression in the murine tumors on transcript and on protein levels. Thus, OPN is a transcriptional target of aberrant Wnt signaling, and OPN expression alone predicts survival in human colon cancer., ((c) 2007 Wiley-Liss, Inc.)

Published
2007
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