1. Reliability of human cortical organoid generation
- Author
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Yoon, Se-Jin, Elahi, Lubayna S, Pașca, Anca M, Marton, Rebecca M, Gordon, Aaron, Revah, Omer, Miura, Yuki, Walczak, Elisabeth M, Holdgate, Gwendolyn M, Fan, H Christina, Huguenard, John R, Geschwind, Daniel H, and Pașca, Sergiu P
- Subjects
Biological Sciences ,Neurosciences ,Stem Cell Research - Induced Pluripotent Stem Cell ,Stem Cell Research - Nonembryonic - Human ,Stem Cell Research - Induced Pluripotent Stem Cell - Human ,Stem Cell Research ,Regenerative Medicine ,Stem Cell Research - Embryonic - Human ,Brain Disorders ,Neurological ,Cell Line ,Humans ,Organoids ,Pluripotent Stem Cells ,Prosencephalon ,Reproducibility of Results ,Sequence Analysis ,RNA ,Single-Cell Analysis ,Tissue Engineering ,Technology ,Medical and Health Sciences ,Developmental Biology ,Biological sciences - Abstract
The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.
- Published
- 2019