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Simple, convenient and universal method of the different types of organosulfur trifluorides synthesis, with common formula RSF3 where R = dialkylamino group, aromatic or heterocyclic radical, was elaborated. The method consists in the reaction of thiosulfurous acid diamides, diaryldisulfides or diheteryldisulfides with chlorine in the presence of potassium fluoride excess. [ABSTRACT FROM AUTHOR]

Arabian show horses are well known for their exceptional beauty and elegance. The breed type, body conformation and movement are assessed during horse shows by licensed judges. The 5 judging categories are type, head and neck, body and topline, legs and movement, which are scored on a 20 point scale. It can be hypothesized that the scores in different categories are related to each other, and that the score for the most subjective type category depends on the scores for conformation categories. We analyzed 762 sets of average scores obtained by 583 unique horses at the World Championships. Correlation analysis and general linear models were used to explore the relationships between the scores in each of the 5 categories. Despite the 20 point scale, only scores from 14.5 to 20 were observed. The correlations between the total score, and the scores for each category within the whole sample varied from r = 0.413 for legs to r = 0.907 for type. Regression analysis of the scores for type showed a strong, significant (P < .001) effect of the scores for the head and neck. The effect of scores for body and topline as well as for movement was negligible, while significant; the score for legs did not affect the score for type. The correlation values between score categories varied between sex and age classes. The estimated levels of correlations were different than expected, based on the known relationships between the phenotypic traits., (Copyright © 2023 Elsevier Inc. All rights reserved.)

The aim of the paper was to analyze impacts of seven years of implementation of the Nagoya Protocol on the livestock sector based on available sources of information and literature. Interim National Reports on implementation of the Nagoya Protocol provided by countries and other information available at the ABS Clearing House managed by the Secretariat of the Convention on Biological Diversity, especially Internationally Recognized Certificates of Compliance (IRCC), were analyzed. Moreover, trends in geneflow of breeding products in selected countries, based on the national reports provided to the UN COMATRADE database, have been compared. Analysis from these sources showed limited impact of the implementation of the Nagoya Protocol for livestock breeding and conservation, as out of 2370 IRCC issued by 31 May 2021, only 573 were granted for animal genetic/biological resources including 90 with livestock as the subject matter. Only one IRCC was granted to a foreign user; all other IRCC were issued for domestic users. The intent was to use livestock genetic/biological resources as bioresources for innovation, which should lead to establishment of the Intellectual Property Rights (IPR) with benefits to be shared through the National Competent Authority or for research purposes.

The domestication of the horse began about 5500 years ago in the Eurasian steppes. In the following millennia horses spread across the ancient world, and their role in transportation and warfare affected every ancient culture. Ownership of horses became an indicator of wealth and social status. The importance of horses led to a growing interest in their breeding and management. Many phenotypic traits, such as height, behavior, and speed potential, have been proven to be a subject of selection; however, the details of ancient breeding practices remain mostly unknown. From the fourth millennium BP, through the Iron Age, many literature sources thoroughly describe horse training systems, as well as various aspects of husbandry, many of which are still in use today. The striking resemblance of ancient and modern equine practices leaves us wondering how much was accomplished through four thousand years of horse breeding.

Some sheep breeds are naturally prolific, and they are very informative for the studies of reproductive genetics and physiology. Major genes increasing litter size (LS) and ovulation rate (OR) were suspected in the French Grivette and the Polish Olkuska sheep populations, respectively. To identify genetic variants responsible for the highly prolific phenotype in these two breeds, genome-wide association studies (GWAS) followed by complementary genetic and functional analyses were performed. Highly prolific ewes (cases) and normal prolific ewes (controls) from each breed were genotyped using the Illumina OvineSNP50 Genotyping Beadchip. In both populations, an X chromosome region, close to the BMP15 gene, harbored clusters of markers with suggestive evidence of association at significance levels between 1E(-05) and 1E(-07). The BMP15 candidate gene was then sequenced, and two novel non-conservative mutations called FecX(Gr) and FecX(O) were identified in the Grivette and Olkuska breeds, respectively. The two mutations were associated with the highly prolific phenotype (p FecX (Gr) = 5.98E(-06) and p FecX(O) = 2.55E(-08)). Homozygous ewes for the mutated allele showed a significantly increased prolificacy (FecX(Gr)/FecX(Gr), LS = 2.50 ± 0.65 versus FecX(+)/FecX(Gr), LS = 1.93 ± 0.42, p<1E(-03) and FecX(O)/FecX(O), OR = 3.28 ± 0.85 versus FecX(+)/FecX(O), OR = 2.02 ± 0.47, p<1E(-03)). Both mutations are located in very well conserved motifs of the protein and altered the BMP15 signaling activity in vitro using a BMP-responsive luciferase test in COV434 granulosa cells. Thus, we have identified two novel mutations in the BMP15 gene associated with increased LS and OR. Notably, homozygous FecX(Gr)/FecX(Gr) Grivette and homozygous FecX(O)/FecX(O) Olkuska ewes are hyperprolific in striking contrast with the sterility exhibited by all other known homozygous BMP15 mutations. Our results bring new insights into the key role played by the BMP15 protein in ovarian function and could contribute to a better understanding of the pathogenesis of women's fertility disorders., Competing Interests: The authors have declared that no competing interests exist.

Recent discoveries that high prolificacy in sheep carrying the Booroola gene (FecB) is the result of a mutation in the BMPIB receptor and high prolificacy in Inverdale sheep (FecX(I)) is the result of a mutation in the BMP15 oocyte-derived growth factor gene have allowed direct marker tests to be developed for FecB and FecX(I). These tests were carried out in seven strains of sheep (Javanese, Thoka, Woodlands, Olkuska, Lacaune, Belclare, and Cambridge) in which inheritance patterns have suggested the presence of major genes affecting prolificacy and in the prolific Garole sheep of India, which have been proposed as the ancestor of Australian Booroola Merinos. The FecB mutation was found in the Garole and Javanese sheep but not in Thoka, Woodlands, Olkuska, Lacaune, Belclare, and Cambridge sheep. None of the sheep tested had the FecX(I) mutation. These findings present strong evidence to support historical records that the Booroola gene was introduced into Australian flocks from Garole (Bengal) sheep in the late 18th century. It is unknown whether Javanese Thin-tailed sheep acquired the Booroola gene directly from Garole sheep from India or via Merinos from Australia. The DNA mutation test for FecB will enable breeding plans to be developed that allow the most effective use of this gene in Garole and Javanese Thin-tailed sheep and their crosses.

Echocardiographic examination of the phase structure of the cardiac cycle was studied in 41 patients with hypertensive disease (stage II) and 37 patients with the hypertensive variant of chronic glomerulonephritis. The authors revealed a certain characterization of blood filling of the left cardiac ventricle at different phases of diastole in these patients. Thus, in patients with hypertensive disease diastolic relaxation of the left ventricle is compensated by an increase of the volume of blood filling during the phase of slow filling and systole phase of the left atrium while in patients with chronic glomerulonephritis compensation occurs mainly due to increased contribution of the blood filling at the systole phase of the left atrium.

A study was made of peroxidation of lipids of erythrocytic biomembranes in 42 patients with chronic glomerulonephritis: 23 patients with the urinary syndrome and 19 with the nephrotic syndrome. Activation of free radical lipid oxidation was shown in both groups of patients; it was especially marked in the group with the nephrotic syndrome. Three degrees of the activity of a renal process were defined on the basis of difference in the concentration of secondary peroxidation products in concrete patients. Correlation between lipid peroxidation indices and expression of proteinuria and leukocyturia was established.