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Jeruk Kalamansi (Citrofortunella microcarpa) merupakan komoditi khas Provinsi Bengkulu yang sekarang banyak dimanfaatkan untuk berbagai pengobatan, makanan dan kosmetika. Minyak atsiri dari limbah Kulit Jeruk Kalamanansi memiliki kandungan senyawa kimia diantaranya yaitu phenyl ethyl alcohol, geraniol, eugenol, dan beberapa senyawa lainnya yang berpotensi memiliki aktivitas antibakteri. Aktivitas antibakteri minyak atsiri kulit Jeruk Kalamansi dapat berpotensi sebagai antibakteri alami pada berbagai macam sediaan kosmetika salah satunya adalah deodorant spray. Tujuan penelitian ini adalah mendapatkan formula deodorant spray mengandung minyak atsiri kulit Jeruk Kalamansi yang memiliki aktivitas antibakteri terhadap bakteri penyebab bau badan yaitu Staphylococcus epidermidis. Dalam penelitian ini dilakukan formulasi deodorant spray dengan 3 formula F1, F2, F3 mengandung minyak atsiri 15%, 20% dan 25% serta F0 basis yang tidak mengandung minyak atsiri dan dibandingkan dengan F4 yaitu deodorant spray merek X yang beredar di pasaran. Pada setiap formula dilakukan uji aktivitas aktibakteri terhadap bakteri Staphylococcus epidermidis pada cawan petri menggunakan metode difusi cakram. Daerah diameter hambat pada masing-masing formula dihitung dan dibandingkan dengan menggunakan metode kualitatif dan kuantitatif. Hasil uji aktivitas antibakteri memperlihatkan bahwa F1 memiliki aktivitas antibakteri paling kuat.

Objective: Medroxyprogesterone Acetate (MPA) using a transdermal drug delivery system for contraception by passive diffusion is limited by the skin barrier properties. Penetration enhancers such as olive oil (fatty acid permeation enhancer) and DMSO (chemical enhancer) can be used. The objective of this study was to overcome MPA penetration problem by using olive oil and DMSO. Methods: An in vitro penetration study using the Franz diffusion cells was performed. The first penetration study used MPA in olive oil (O) and MPA in coconut oil (C) with the concentration 100 μg/ml to each sample and MPA suspension as a control with the same concentration. The second study used MPA in olive oil with the concentration 200.0 μg/ml (A), MPA in olive oil with 0.5% DMSO with the concentration 200.0 μg/ml (B), and MPA in olive oil with 1% DMSO with the concentration 200 μg/ml (C). Results: MPA penetration test for olive oil+0.5% DMSO had flux value 4.24±0.074 μg/cm2. hr and it was not significantly different (t-test, P>0.05) with olive oil+1% DMSO. While the MPA penetration test in only Olive oil had flux value 0.90±0.0087 μg/cm2. hr. Conclusion: This research concluded that olive oil and 0.5% DMSO could improve the penetration of MPA into skin membrane by 4.5 times more than olive oil alone.

Objective: The aim of this study was to develop transferosome vesicles for the transdermal drug delivery of lynestrenol.Methods: The lynestrenol transferosome vesicle was made by encapsulating the drug in a variation of phosphatidylcholine and Tween 80 by the thinlayerhydration method. The resulting transferosome vesicles were modified with a time variation of 30, 60, 90, and 120 min, and sonication variationswere paused and not paused. Particle size evaluation, polydispersity (PDI), and entrapment efficiency (%EE) were carried out on the variation ofsonication time.Results: The evaluation results showed that sonication without pauses showed better %EE and particle size than sonication with pauses andincreasing concentration of Tween 80 (edge activator). The %EE increased, and particle size decreased with increasing sonication time; PDI of vesicleswas heterogeneous with increasing sonication time. The %EE in formulas F1 and F2 after 120 min was 73.06% and 76.06% (paused) and 80.40% and82.97% (without paused). The particle size of formula F1 and F2 after 120 min 575.4 nm and 471.6 nm (paused) and 524.1 nm and 434.7 nm (withoutpaused). The PDI formulas of F1 and F2 after 120 min were 0.69 and 0.763 (paused) and 0.84 and 0.59 (without paused).Conclusion: Based on the results of the transferosome vesicle characteristics, it was shown that the optimal vesicle composition for packaginglynestrenol was vesicles that were composed of phosphatidylcholine and Tween 80 without pauses and could potentially be used as a transdermaldrug delivery system.

The determination of pseudoephedrine hydrochloride and triprolidine hydro-chloride in influenza syrup medicine has been performed using TLC densitometric method. Pseudoephedrine hydrochloride and triprolidine hydrochloride were extracted using chloroform at pH 12 from the syrup, and separated using HPTLC silica Kie-selguhr glass plates 60 F 254, 20x10 cm2 as stationary phase, and a mixture of methanol, ammonia and chloroform (40:2:30) as mobile phase. The plates were ana-lyzed using Camag TLC Scanner 3 with UV-detector at 257 nm for pseudoephe-drine hydrochloride and at 290 nm for triprolidine hydrochloride. The results showed that the linearity, limit of detection, and limit of quantitation of the method for pseudoephedrine hydrochloride were 0.9999, 0.0064 µg, and 0.2124 µg respectively; while for triprolidine hydrochloride were 0.9999, 0.0076 µg, and 0.0254 µg respec-tively. The coefficient of variance (CV) of repeatability for the two substances were less than 2.0%; and the recovery values for pseudoepherine hydrochloride and triprolidine hydrochloride were 99.98 + 1.05% and 99.73 + 1,54% respectively. The result showed that the samples analysed contained pseudoephedrine hydrochloride 94.36% of the labeled ammount, and triprolidine hydrochloride 94.44% of the la-beled ammount. Key words : pseudoephedrine hydrochloride, triprolidine hydrochloride, TLC densitometric method.