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1. Fungal Planet description sheets : 951-1041

Author

Crous, P. W., Wingfield, M. J., Lombard, L., Roets, F., Swart, W. J., Alvarado, P., Carnegie, A. J., Moreno, G., Luangsa-ard, J., Thangavel, R., Alexandrova, A. V., Baseia, I. G., Bellanger, J. -M, Bessette, A. E., Bessette, A. R., De la Pena-Lastra, S., Garcia, D., Gene, J., Pham, T. H. G., Heykoop, M., Malysheva, E., Malysheva, V., Martin, M. P., Morozova, O. V., Noisripoom, W., Overton, B. E., Rea, A. E., Sewall, B. J., Smith, M. E., Smyth, C. W., Tasanathai, K., Visagie, C. M., Adamcik, S., Alves, A., Andrade, J. P., Aninat, M. J., Araujo, R. V. B., Bordallo, J. J., Boufleur, T., Baroncelli, R., Barreto, R. W., Bolin, J., Cabero, J., Cabon, M., Cafa, G., Caffot, M. L. H., Cai, L., Carlavilla, J. R., Chavez, R., de Castro, R. R. L., Delgat, L., Deschuyteneer, D., Dios, M. M., Dominguez, L. S., Evans, H. C., Eyssartier, G., Ferreira, B. W., Figueiredo, C. N., Liu, F., Fournier, J., Galli-Terasawa, L. V., Gil-Duran, C., Glienke, C., Goncalves, M. F. M., Gryta, H., Guarro, J., Himaman, W., Hywel-Jones, N., Iturrieta-Gonzalez, I., Ivanushkina, N. E., Jargeat, P., Khalid, A. N., Khan, J., Kiran, M., Kiss, L., Kochkina, G. A., Kolarik, M., Kubatova, A., Lodge, D. J., Loizides, M., Luque, D., Manjon, J. L., Marbach, P. A. S., Massola, N. S., Jr., Mata, M., Miller, A. N., Mongkolsamrit, S., Moreau, P. -A, Morte, A., Mujic, A., Navarro-Rodenas, A., Nemeth, M. Z., Nobrega, T. F., Novakova, A., Olariaga, I., Ozerskaya, S. M., Palma, M. A., Petters-Vandresen, D. A. L., Piontelli, E., Popov, E. S., Rodriguez, A., Requejo, O., Rodrigues, A. C. M., Rong, I. H., Roux, J., Seifert, K. A., Silva, B. D. B., Sklenar, F., Smith, J. A., Sousa, J. O., Souza, H. G., De Souza, J. T., Svec, K., Tanchaud, P., Tanney, J. B., Terasawa, F., Thanakitpipattana, D., Torres-Garcia, D., Vaca, I., Vaghefi, N., van Iperen, A. L., Vasilenko, O. V., Verbeken, A., Yilmaz, N., Zamora, Juan Carlos, Zapata, M., Jurjevic, Z., Groenewald, J. Z., Crous, P. W., Wingfield, M. J., Lombard, L., Roets, F., Swart, W. J., Alvarado, P., Carnegie, A. J., Moreno, G., Luangsa-ard, J., Thangavel, R., Alexandrova, A. V., Baseia, I. G., Bellanger, J. -M, Bessette, A. E., Bessette, A. R., De la Pena-Lastra, S., Garcia, D., Gene, J., Pham, T. H. G., Heykoop, M., Malysheva, E., Malysheva, V., Martin, M. P., Morozova, O. V., Noisripoom, W., Overton, B. E., Rea, A. E., Sewall, B. J., Smith, M. E., Smyth, C. W., Tasanathai, K., Visagie, C. M., Adamcik, S., Alves, A., Andrade, J. P., Aninat, M. J., Araujo, R. V. B., Bordallo, J. J., Boufleur, T., Baroncelli, R., Barreto, R. W., Bolin, J., Cabero, J., Cabon, M., Cafa, G., Caffot, M. L. H., Cai, L., Carlavilla, J. R., Chavez, R., de Castro, R. R. L., Delgat, L., Deschuyteneer, D., Dios, M. M., Dominguez, L. S., Evans, H. C., Eyssartier, G., Ferreira, B. W., Figueiredo, C. N., Liu, F., Fournier, J., Galli-Terasawa, L. V., Gil-Duran, C., Glienke, C., Goncalves, M. F. M., Gryta, H., Guarro, J., Himaman, W., Hywel-Jones, N., Iturrieta-Gonzalez, I., Ivanushkina, N. E., Jargeat, P., Khalid, A. N., Khan, J., Kiran, M., Kiss, L., Kochkina, G. A., Kolarik, M., Kubatova, A., Lodge, D. J., Loizides, M., Luque, D., Manjon, J. L., Marbach, P. A. S., Massola, N. S., Jr., Mata, M., Miller, A. N., Mongkolsamrit, S., Moreau, P. -A, Morte, A., Mujic, A., Navarro-Rodenas, A., Nemeth, M. Z., Nobrega, T. F., Novakova, A., Olariaga, I., Ozerskaya, S. M., Palma, M. A., Petters-Vandresen, D. A. L., Piontelli, E., Popov, E. S., Rodriguez, A., Requejo, O., Rodrigues, A. C. M., Rong, I. H., Roux, J., Seifert, K. A., Silva, B. D. B., Sklenar, F., Smith, J. A., Sousa, J. O., Souza, H. G., De Souza, J. T., Svec, K., Tanchaud, P., Tanney, J. B., Terasawa, F., Thanakitpipattana, D., Torres-Garcia, D., Vaca, I., Vaghefi, N., van Iperen, A. L., Vasilenko, O. V., Verbeken, A., Yilmaz, N., Zamora, Juan Carlos, Zapata, M., Jurjevic, Z., and Groenewald, J. Z.

Abstract

Novel species of fungi described in this study include those from various countries as follows: Antarctica,Apenidiella antarctica from permafrost, Cladosporium fildesense from an unidentified marine sponge. Argentina,Geastrum wrightii on humus in mixed forest. Australia, Golovinomyces glandulariae on Glandularia aristigera,Neoanungitea eucalyptorum on leaves of Eucalyptus grandis, Teratosphaeria corymbiicola on leaves of Corymbiaficifolia, Xylaria eucalypti on leaves of Eucalyptus radiata. Brazil, Bovista psammophila on soil, Fusarium awaxy onrotten stalks of Zea mays, Geastrum lanuginosum on leaf litter covered soil, Hermetothecium mikaniae-micranthae(incl. Hermetothecium gen. nov.) on Mikania micrantha, Penicillium reconvexovelosoi in soil, Stagonosporopsis vannacciifrom pod of Glycine max. British Virgin Isles, Lactifluus guanensis on soil. Canada, Sorocybe oblongisporaon resin of Picea rubens. Chile, Colletotrichum roseum on leaves of Lapageria rosea. China, Setophoma cavernafrom carbonatite in Karst cave. Colombia, Lareunionomyces eucalypticola on leaves of Eucalyptus grandis. CostaRica, Psathyrella pivae on wood. Cyprus, Clavulina iris on calcareous substrate. France, Chromosera ambiguaand Clavulina iris var. occidentalis on soil. French West Indies, Helminthosphaeria hispidissima on dead wood.Guatemala, Talaromyces guatemalensis in soil. Malaysia, Neotracylla pini (incl. Tracyllales ord. nov. and Neotracyllagen. nov.) and Vermiculariopsiella pini on needles of Pinus tecunumanii. New Zealand, Neoconiothyriumviticola on stems of Vitis vinifera, Parafenestella pittospori on Pittosporum tenuifolium, Pilidium novae-zelandiaeon Phoenix sp. Pakistan, Russula quercus-floribundae on forest floor. Portugal, Trichoderma aestuarinum fromsaline water. Russia, Pluteus liliputianus on fallen branch of deciduous tree, Pluteus spurius on decaying deciduous wood or soil. South Africa, Alloconiothyrium encephalarti, Phyllosticta encephalarticola and Neothyrostromaencephalarti (

Published
2019
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2. Fungal Planet description sheets: 951-1041

Author

Universitat Rovira i Virgili, Crous, P. W.; Wingfield, M. J.; Lombard, L.; Roets, F.; Swart, W. J.; Alvarado, P.; Carnegie, A. J.; Moreno, G.; Luangsa-ard, J.; Thangavel, R.; Alexandrova, A. V.; Baseia, I. G.; Bellanger, J. -M.; Bessette, A. E.; Bessette, A. R.; De la Pena-Lastra, S.; Garcia, D.; Gene, J.; Pham, T. H. G.; Heykoop, M.; Malysheva, E.; Malysheva, V.; Martin, M. P.; Morozova, O. V.; Noisripoom, W.; Overton, B. E.; Rea, A. E.; Sewall, B. J.; Smith, M. E.; Smyth, C. W.; Tasanathai, K.; Visagie, C. M.; Adamcik, S.; Alves, A.; Andrade, J. P.; Aninat, M. J.; Araujo, R. V. B.; Bordallo, J. J.; Boufleur, T.; Baroncelli, R.; Barreto, R. W.; Bolin, J.; Cabero, J.; Cabon, M.; Cafa, G.; Caffot, M. L. H.; Cai, L.; Carlavilla, J. R.; Chavez, R.; de Castro, R. R. L.; Delgat, L.; Deschuyteneer, D.; Dios, M. M.; Dominguez, L. S.; Evans, H. C.; Eyssartier, G.; Ferreira, B. W.; Figueiredo, C. N.; Liu, F.; Fournier, J.; Galli-Terasawa, L. V.; Gil-Duran, C.; Glienke, C.; Goncalves, M. F. M.; Gryta, H.; Guarro, J.; Himaman, W.; Hywel-Jones, N.; Iturrieta-Gonzalez, I.; Ivanushkina, N. E.; Jargeat, P.; Khalid, A. N.; Khan, J.; Kiran, M.; Kiss, L.; Kochkina, G. A.; Kolarik, M.; Kubatova, A.; Lodge, D. J.; Loizides, M.; Luque, D.; Manjon, J. L.; Marbach, P. A. S.; Massola, N. S., Jr.; Mata, M.; Miller, A. N.; Mongkolsamrit, S.; Moreau, P. -A.; Morte, A.; Mujic, A.; Navarro-Rodenas, A.; Nemeth, M. Z.; Nobrega, T. F.; Novakova, A.; Olariaga, I.; Ozerskaya, S. M.; Palma, M. A.; Petters-Vandresen, D. A. L.; Piontelli, E.; Popov, E. S.; Rodriguez, A.; Requejo, O.; Rodrigues, A. C. M.; Rong, I. H.; Roux, J.; Seifert, K. A.; Silva, B. D. B.; Sklenar, F.; Smith, J. A.; Sousa, J. O.; Souza, H. G.; De Souza, J. T.; Svec, K.; Tanchaud, P.; Tanney, J. B.; Terasawa, F.; Thanakitpipattana, D.; Torres-Garcia, D.; Vaca, I.; Va, Universitat Rovira i Virgili, and Crous, P. W.; Wingfield, M. J.; Lombard, L.; Roets, F.; Swart, W. J.; Alvarado, P.; Carnegie, A. J.; Moreno, G.; Luangsa-ard, J.; Thangavel, R.; Alexandrova, A. V.; Baseia, I. G.; Bellanger, J. -M.; Bessette, A. E.; Bessette, A. R.; De la Pena-Lastra, S.; Garcia, D.; Gene, J.; Pham, T. H. G.; Heykoop, M.; Malysheva, E.; Malysheva, V.; Martin, M. P.; Morozova, O. V.; Noisripoom, W.; Overton, B. E.; Rea, A. E.; Sewall, B. J.; Smith, M. E.; Smyth, C. W.; Tasanathai, K.; Visagie, C. M.; Adamcik, S.; Alves, A.; Andrade, J. P.; Aninat, M. J.; Araujo, R. V. B.; Bordallo, J. J.; Boufleur, T.; Baroncelli, R.; Barreto, R. W.; Bolin, J.; Cabero, J.; Cabon, M.; Cafa, G.; Caffot, M. L. H.; Cai, L.; Carlavilla, J. R.; Chavez, R.; de Castro, R. R. L.; Delgat, L.; Deschuyteneer, D.; Dios, M. M.; Dominguez, L. S.; Evans, H. C.; Eyssartier, G.; Ferreira, B. W.; Figueiredo, C. N.; Liu, F.; Fournier, J.; Galli-Terasawa, L. V.; Gil-Duran, C.; Glienke, C.; Goncalves, M. F. M.; Gryta, H.; Guarro, J.; Himaman, W.; Hywel-Jones, N.; Iturrieta-Gonzalez, I.; Ivanushkina, N. E.; Jargeat, P.; Khalid, A. N.; Khan, J.; Kiran, M.; Kiss, L.; Kochkina, G. A.; Kolarik, M.; Kubatova, A.; Lodge, D. J.; Loizides, M.; Luque, D.; Manjon, J. L.; Marbach, P. A. S.; Massola, N. S., Jr.; Mata, M.; Miller, A. N.; Mongkolsamrit, S.; Moreau, P. -A.; Morte, A.; Mujic, A.; Navarro-Rodenas, A.; Nemeth, M. Z.; Nobrega, T. F.; Novakova, A.; Olariaga, I.; Ozerskaya, S. M.; Palma, M. A.; Petters-Vandresen, D. A. L.; Piontelli, E.; Popov, E. S.; Rodriguez, A.; Requejo, O.; Rodrigues, A. C. M.; Rong, I. H.; Roux, J.; Seifert, K. A.; Silva, B. D. B.; Sklenar, F.; Smith, J. A.; Sousa, J. O.; Souza, H. G.; De Souza, J. T.; Svec, K.; Tanchaud, P.; Tanney, J. B.; Terasawa, F.; Thanakitpipattana, D.; Torres-Garcia, D.; Vaca, I.; Va

Abstract

Novel species of fungi described in this study include those from various countries as follows: Antarctica,Apenidiella antarctica from permafrost, Cladosporium fildesense from an unidentified marine sponge. Argentina,Geastrum wrightii on humus in mixed forest. Australia, Golovinomyces glandulariae on Glandularia aristigera,Neoanungitea eucalyptorum on leaves of Eucalyptus grandis, Teratosphaeria corymbiicola on leaves of Corymbiaficifolia, Xylaria eucalypti on leaves of Eucalyptus radiata. Brazil, Bovista psammophila on soil, Fusarium awaxy onrotten stalks of Zea mays, Geastrum lanuginosum on leaf litter covered soil, Hermetothecium mikaniae-micranthae(incl. Hermetothecium gen. nov.) on Mikania micrantha, Penicillium reconvexovelosoi in soil, Stagonosporopsis vannacciifrom pod of Glycine max. British Virgin Isles, Lactifluus guanensis on soil. Canada, Sorocybe oblongisporaon resin of Picea rubens. Chile, Colletotrichum roseum on leaves of Lapageria rosea. China, Setophoma cavernafrom carbonatite in Karst cave. Colombia, Lareunionomyces eucalypticola on leaves of Eucalyptus grandis. CostaRica, Psathyrella pivae on wood. Cyprus, Clavulina iris on calcareous substrate. France, Chromosera ambiguaand Clavulina iris var. occidentalis on soil. French West Indies, Helminthosphaeria hispidissima on dead wood.Guatemala, Talaromyces guatemalensis in soil. Malaysia, Neotracylla pini (incl. Tracyllales ord. nov. and Neotracyllagen. nov.) and Vermiculariopsiella pini on needles of Pinus tecunumanii. New Zealand, Neoconiothyriumviticola on stems of Vitis vinifera, Parafenestella pittospori on Pittosporum tenuifolium, Pilidium novae-zelandiaeon Phoenix sp. Pakistan, Russula quercus-floribundae on forest floor. Portugal, Trichoderma aestuarinum fromsaline water. Russia, Pluteus liliputi

Published
2019

3. Fungal Planet description sheets: 951-1041.

Author

Crous PW, Wingfield MJ, Lombard L, Roets F, Swart WJ, Alvarado P, Carnegie AJ, Moreno G, Luangsaard J, Thangavel R, Alexandrova AV, Baseia IG, Bellanger JM, Bessette AE, Bessette AR, De la Peña-Lastra S, García D, Gené J, Pham THG, Heykoop M, Malysheva E, Malysheva V, Martín MP, Morozova OV, Noisripoom W, Overton BE, Rea AE, Sewall BJ, Smith ME, Smyth CW, Tasanathai K, Visagie CM, Adamčík S, Alves A, Andrade JP, Aninat MJ, Araújo RVB, Bordallo JJ, Boufleur T, Baroncelli R, Barreto RW, Bolin J, Cabero J, Caboň M, Cafà G, Caffot MLH, Cai L, Carlavilla JR, Chávez R, de Castro RRL, Delgat L, Deschuyteneer D, Dios MM, Domínguez LS, Evans HC, Eyssartier G, Ferreira BW, Figueiredo CN, Liu F, Fournier J, Galli-Terasawa LV, Gil-Durán C, Glienke C, Gonçalves MFM, Gryta H, Guarro J, Himaman W, Hywel-Jones N, Iturrieta-González I, Ivanushkina NE, Jargeat P, Khalid AN, Khan J, Kiran M, Kiss L, Kochkina GA, Kolařík M, Kubátová A, Lodge DJ, Loizides M, Luque D, Manjón JL, Marbach PAS, Massola NS Jr, Mata M, Miller AN, Mongkolsamrit S, Moreau PA, Morte A, Mujic A, Navarro-Ródenas A, Németh MZ, Nóbrega TF, Nováková A, Olariaga I, Ozerskaya SM, Palma MA, Petters-Vandresen DAL, Piontelli E, Popov ES, Rodríguez A, Requejo Ó, Rodrigues ACM, Rong IH, Roux J, Seifert KA, Silva BDB, Sklenář F, Smith JA, Sousa JO, Souza HG, De Souza JT, Švec K, Tanchaud P, Tanney JB, Terasawa F, Thanakitpipattana D, Torres-Garcia D, Vaca I, Vaghefi N, van Iperen AL, Vasilenko OV, Verbeken A, Yilmaz N, Zamora JC, Zapata M, Jurjević Ž, and Groenewald JZ

Abstract

Novel species of fungi described in this study include those from various countries as follows: Antarctica , Apenidiella antarctica from permafrost, Cladosporium fildesense from an unidentified marine sponge. Argentina , Geastrum wrightii on humus in mixed forest. Australia , Golovinomyces glandulariae on Glandularia aristigera, Neoanungitea eucalyptorum on leaves of Eucalyptus grandis, Teratosphaeria corymbiicola on leaves of Corymbia ficifolia, Xylaria eucalypti on leaves of Eucalyptus radiata. Brazil , Bovista psammophila on soil, Fusarium awaxy on rotten stalks of Zea mays, Geastrum lanuginosum on leaf litter covered soil, Hermetothecium mikaniae-micranthae (incl. Hermetothecium gen. nov.) on Mikania micrantha, Penicillium reconvexovelosoi in soil, Stagonosporopsis vannaccii from pod of Glycine max. British Virgin Isles , Lactifluus guanensis on soil. Canada , Sorocybe oblongispora on resin of Picea rubens. Chile , Colletotrichum roseum on leaves of Lapageria rosea. China , Setophoma caverna from carbonatite in Karst cave. Colombia , Lareunionomyces eucalypticola on leaves of Eucalyptus grandis. Costa Rica , Psathyrella pivae on wood. Cyprus , Clavulina iris on calcareous substrate. France , Chromosera ambigua and Clavulina iris var. occidentalis on soil. French West Indies , Helminthosphaeria hispidissima on dead wood. Guatemala , Talaromyces guatemalensis in soil. Malaysia , Neotracylla pini (incl. Tracyllales ord. nov. and Neotracylla gen. nov.) and Vermiculariopsiella pini on needles of Pinus tecunumanii. New Zealand , Neoconiothyrium viticola on stems of Vitis vinifera, Parafenestella pittospori on Pittosporum tenuifolium, Pilidium novae-zelandiae on Phoenix sp. Pakistan , Russula quercus-floribundae on forest floor. Portugal , Trichoderma aestuarinum from saline water. Russia , Pluteus liliputianus on fallen branch of deciduous tree, Pluteus spurius on decaying deciduous wood or soil. South Africa , Alloconiothyrium encephalarti, Phyllosticta encephalarticola and Neothyrostroma encephalarti (incl. Neothyrostroma gen. nov.) on leaves of Encephalartos sp., Chalara eucalypticola on leaf spots of Eucalyptus grandis × urophylla, Clypeosphaeria oleae on leaves of Olea capensis, Cylindrocladiella postalofficium on leaf litter of Sideroxylon inerme , Cylindromonium eugeniicola (incl. Cylindromonium gen. nov.) on leaf litter of Eugenia capensis , Cyphellophora goniomatis on leaves of Gonioma kamassi , Nothodactylaria nephrolepidis (incl. Nothodactylaria gen. nov. and Nothodactylariaceae fam. nov.) on leaves of Nephrolepis exaltata , Falcocladium eucalypti and Gyrothrix eucalypti on leaves of Eucalyptus sp., Gyrothrix oleae on leaves of Olea capensis subsp. macrocarpa , Harzia metrosideri on leaf litter of Metrosideros sp., Hippo pota myces phragmitis (incl. Hippo pota myces gen. nov.) on leaves of Phragmites australis , Lectera philenopterae on Philenoptera violacea , Leptosillia mayteni on leaves of Maytenus heterophylla , Lithohypha aloicola and Neoplatysporoides aloes on leaves of Aloe sp., Millesimomyces rhoicissi (incl. Millesimomyces gen. nov.) on leaves of Rhoicissus digitata , Neodevriesia strelitziicola on leaf litter of Strelitzia nicolai , Neokirramyces syzygii (incl. Neokirramyces gen. nov.) on leaf spots of Syzygium sp., Nothoramichloridium perseae (incl. Nothoramichloridium gen. nov. and Anungitiomycetaceae fam. nov.) on leaves of Persea americana , Paramycosphaerella watsoniae on leaf spots of Watsonia sp., Penicillium cuddlyae from dog food, Podocarpomyces knysnanus (incl. Podocarpomyces gen. nov.) on leaves of Podocarpus falcatus , Pseudocercospora heteropyxidicola on leaf spots of Heteropyxis natalensis , Pseudopenidiella podocarpi, Scolecobasidium podocarpi and Ceramothyrium podocarpicola on leaves of Podocarpus latifolius , Scolecobasidium blechni on leaves of Blechnum capense , Stomiopeltis syzygii on leaves of Syzygium chordatum , Strelitziomyces knysnanus (incl. Strelitziomyces gen. nov.) on leaves of Strelitzia alba , Talaromyces clemensii from rotting wood in goldmine, Verrucocladosporium visseri on Carpobrotus edulis. Spain , Boletopsis mediterraneensis on soil, Calycina cortegadensisi on a living twig of Castanea sativa , Emmonsiellopsis tuberculata in fluvial sediments, Mollisia cortegadensis on dead attached twig of Quercus robur , Psathyrella ovispora on soil, Pseudobeltrania lauri on leaf litter of Laurus azorica , Terfezia dunensis in soil, Tuber lucentum in soil, Venturia submersa on submerged plant debris. Thailand , Cordyceps jakajanicola on cicada nymph, Cordyceps kuiburiensis on spider, Distoseptispora caricis on leaves of Carex sp., Ophiocordyceps khonkaenensis on cicada nymph. USA , Cytosporella juncicola and Davidiellomyces juncicola on culms of Juncus effusus , Monochaetia massachusettsianum from air sample, Neohelicomyces melaleucae and Periconia neobrittanica on leaves of Melaleuca styphelioides × lanceolata , Pseudocamarosporium eucalypti on leaves of Eucalyptus sp . , Pseudogymnoascus lindneri from sediment in a mine, Pseudogymnoascus turneri from sediment in a railroad tunnel, Pulchroboletus sclerotiorum on soil, Zygosporium pseudomasonii on leaf of Serenoa repens. Vietnam , Boletus candidissimus and Veloporphyrellus vulpinus on soil. Morphological and culture characteristics are supported by DNA barcodes., (© 2019 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute.)

Published
2019
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4. Phylogeny and variability of Colletotrichum truncatum associated with soybean anthracnose in Brazil.

Author

Rogério F, Ciampi-Guillardi M, Barbieri MC, Bragança CA, Seixas CD, Almeida AM, and Massola NS Jr

Subjects
Bayes Theorem, Brazil, Colletotrichum classification, Colletotrichum cytology, Colletotrichum genetics, DNA, Fungal genetics, DNA, Ribosomal, Genetic Variation, Geography, Phylogeny, Glycine max genetics, Spores, Fungal classification, Spores, Fungal cytology, Spores, Fungal isolation & purification, Colletotrichum isolation & purification, Plant Diseases microbiology, Glycine max microbiology
Abstract

Aims: Fungal diseases are among the main factors limiting high yields of soybean crop. Colletotrichum isolates from soybean plants with anthracnose symptoms were studied from different regions and time periods in Brazil using molecular, morphological and pathogenic analyses., Methods and Results: Bayesian phylogenetic inference of GAPDH, HIS3 and ITS-5.8S rDNA sequences, the morphologies of colony and conidia, and inoculation tests on seeds and seedlings were performed. All isolates clustered only with Colletotrichum truncatum species in three well-separated clusters. Intraspecific genetic diversity revealed 27 distinct haplotypes in 51 fungal isolates; some of which were identical to C. truncatum sequences from other regions around the world, while others were related to alternative hosts. Conidia were falcate, hyaline, unicellular and aseptate, formed in acervuli, with variable dimensions. Despite being pathogenic to seedlings by both inoculation methods, variation was observed in the aggressiveness of the tested isolates, which was not correlated with genetic variation., Conclusion: The identification of C. truncatum in the sampled isolates was evidenced as being the only causal agent of soybean anthracnose in Brazil until 2007, with relevant genetic, morphological and pathogenic variability as well as a broad geographical origin. The wide distribution of the predominant C. truncatum haplotype indicated the existence of a highly efficient mechanism of pathogen dispersal over long distances, reinforcing the role of seeds as the primary source of disease inoculum., Significance and Impact of the Study: The characterization and distribution of Colletotrichum species in soybean-producing regions in Brazil is fundamental for understanding the disease epidemiology and for ensuring effective control strategies against anthracnose., (© 2016 The Society for Applied Microbiology.)

Published
2017
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5. First Report of Anthracnose Caused by Colletotrichum theobromicola on Barbados Cherry (Malpighia emarginata) in Brazil.

Author

Bragança CAD, Junior AFN, Rogério F, and Massola NS Jr

Abstract

Barbados cherry, also called acerola, is a fruit originated from tropical America that is well-known for its high content of vitamin C and nutritional value. Anthracnose is one of the most common diseases on Barbados cherry. In Brazil, this disease is associated with Colletotrichum gloeosporioides sensu lato (2). In 2012, necrotic and sunken spots were observed on Barbados cherry fruit (cv. Rubra) in Sao Paulo State, Brazil, from which a Colletotrichum species was isolated on potato dextrose agar (PDA). The isolate was grown on PDA at 25°C and 12-h photoperiod under fluorescent light. The colony was gray on the upper surface and the reverse part was dark gray. Conidia (n = 50) were cylindrical to subcylindrical, hyaline, and 12 to 15 (avg. 12.7) × 3.8 to 5.9 (avg. 4.3) μm. Conidia length/width ratio was 2 to 3.6. Pathogenicity was confirmed on Barbados cherry fruit. Inoculation was carried out by depositing 40-μl droplets of a conidial suspension (1 × 10 5 conidia ml -1 ) on fruit wounded with a sterilized needle and on non-wounded fruit. Fruit were incubated in a moist chamber at 25°C. First symptoms appeared 3 and 5 days after inoculation on wounded and non-wounded fruit, respectively. No symptoms were observed on control fruit inoculated with water. Six isolates recovered from inoculated fruit showed the same morphological characteristics of the previous isolate. The DNA of the fungus was extracted by a CTAB protocol (1) and the sequences of ITS, GAPDH, ACT, CHS-1, TUB, and CAL genes (4) were generated. Sequences were used in BLAST searches in GenBank and were 100% similar to C. theobromicola, except for GAPDH. The ITS (KC566724) and CAL (KC566437) sequences matched strain ICMP 17099 (JX010285 and JX009588, respectively) with 100% identity. The BTUB (KC566148), GAPDH (KC566578), ACT (KC566870), and CHS-1(KC566292) sequences matched with the strains ICMP 18649 (JX010447, 100% identity), ICMP 17099 (JX009957, 99% identity, 1 pb), ICMP 18567 (JX009457, 100% identity), and ICMP 18613 (JX009771, 100% identity), respectively. The sequences were also compared with authentic culture of C. gloeosporioides (IMI 356878) and the identities were: ITS 99% (JX010148), CAL 91% (JX009729), BTUB 90% (JX010445), GAPDH 83% (GU174561), ACT 93% (JX009494), and CHS-1 98% (JX009747). Based on the multi-gene sequencing, the isolate was identified as C. theobromicola. C. theobromicola was described in 2010 (3) and it is considered as a widely distributed species occurring on different hosts in tropical and subtropical regions (4). This report shows the necessity of the identification of Colletotrichum species from tropical fruits to elucidate the etiology of anthracnose diseases of which C. gloeosporioides sensu lato is considered to be the causal agent. To our knowledge, this is the first report of C. theobromicola on Barbados cherry. References: (1) M. G. Murray and W. F. Thompson. Nucleic Acids Res. 8:4321, 1980. (2) R. Ritzinger et al. Acerola em Foco 13:1, 2007. (3) E. I. Rojas et al. Mycologia 102:1318, 2010. (4) B. S. Weir et al. Stud. Mycol. 73:115, 2012.

Published
2014
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