1. Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells
- Author
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Anouk Mentink, Marloes Levers, Frédéric Mourcin, Nick R.M. Beijer, Karin Tarte, Frits Hulshof, Dimitrios Stamatialis, Bernke J. Papenburg, Jan de Boer, Aliaksei Vasilevich, Anne E. Carpenter, Yiping Zhao, Clemens van Blitterswijk, Shantanu Singh, Biointerface Science, RS: MERLN - Cell Biology - Inspired Tissue Engineering (CBITE), CBITE, CTR, RS: MERLN - Complex Tissue Regeneration (CTR), Laboratory for Cell Biology-Inspired Tissue Engineering [Maastricht, Netherlands], Maastricht University [Maastricht]-MERLN Institute for Technology-Inspired Regenerative Medicine [Maastricht, Netherlands], Microenvironnement et cancer (MiCa), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Equipe Labelisée Ligue Contre le Cancer [Rennes], Etablissement français du sang [Rennes] (EFS Bretagne)-Université de Rennes, Department of Biomaterials Science and Technology [Maastricht, Netherlands], University of Twente [Netherlands]-MIRA Institute for Biomedical Technology and Technical Medicine [Maastricht, Netherlands], Materiomics BV [Maastricht, Netherlands], Imaging Platform [Cambridge, MA, USA], Broad Institute of MIT and Harvard (BROAD INSTITUTE), Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston]-Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston], Department of Complex Tissue Regeneration [Maastricht, Netherlands], R35 GM122547, GM, NIGMS NIH HHS, United States, Biomaterials Science and Technology, Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Etablissement français du sang [Rennes] (EFS Bretagne)-Université de /Rennes, University of Twente-MIRA Institute for Biomedical Technology and Technical Medicine [Maastricht, Netherlands], and Jonchère, Laurent
- Subjects
0301 basic medicine ,Histology ,Stromal cell ,ICAM-1 ,lcsh:Biotechnology ,Biomedical Engineering ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Bioengineering ,Cell morphology ,Flow cytometry ,03 medical and health sciences ,Mechanobiology ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Downregulation and upregulation ,Reticular cell ,lcsh:TP248.13-248.65 ,medicine ,Lymph node ,Original Research ,medicine.diagnostic_test ,Chemistry ,Bioengineering and Biotechnology ,mechanobiology ,surface topography ,lymph node ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,fibroblastic reticular cells ,Biotechnology - Abstract
International audience; Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.
- Published
- 2018
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