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Background: Dysbiosis of the gut microbiota has been demonstrated in neurodevelopmental disorders but the underlying mechanisms that may explain these associations are poorly understood. Gut secretory immunoglobulin A (SIgA) binds pathogenic microbes, preventing mucosal penetration. Gut microbes also influence SIgA production and its binding characteristics through short-chain fatty acid (SCFA) metabolites, allowing them to regulate the immune response. Serum IgA deficiency has been noted in children with autism spectrum disorders (ASD). In this study, we aimed to determine whether SIgA level in infancy is associated with gut microbiota taxonomy and metabolites, and neurodevelopmental outcomes in preschool children. Methods: For a subsample of 178 children from the Canadian CHILD Cohort Study, gut microbiota of fecal samples collected at 3–4 months and 12 months was profiled using 16S rRNA sequencing. Gut bacterial metabolites levels and SIgA level were measured by nuclear magnetic resonance (NMR) based metabolomics and SIgA enzyme-linked immunosorbent assay at 3–4 months, respectively. Bayley-III Scale of Infant Development was assessed at 12 and 24 months. We evaluated direct relationships in multiple linear regression models and putative causal relationships in statistical mediation models. Results: Propionate and butyrate levels at 3–4 months were associated with decreased Bayley cognitive score at 24 months (p-values: 0.01 and 0.02, respectively) in adjusted multiple linear regression models, but when we investigated an indirect relationship mediated by decreased SIgA level at 3–4 months, it did not reach statistical significance (p-values: 0.18 and 0.20, respectively). Lactate level at 3–4 months was associated with increased Bayley cognitive score at 24 months in adjusted multiple linear regression models (p-value: 0.01), but the statistical model mediated by increased SIgA level at 3–4 months did not reach statistical significance neither (p-value: 0.20). Conclusions: Our study contributes to growing evidence that neurodevelopment is influenced by the infant gut microbiota and that it might involve SIgA level, but larger studies are required. more...

Alluvial plain landscapes are some of the most agriculturally productive lands in the world but often have modified stream ecosystems due to cultivation history. This context requires consideration when establishing water quality management goals. We analyzed state water quality databases to demonstrate that Mississippi Alluvial Plain (MAP) ecoregion streams have elevated specific conductivity (SC) and nutrients and lower macroinvertebrate local and regional taxa pools compared to streams in other ecoregions, potentially reducing the efficacy of traditional biomonitoring approaches within the region. To overcome these challenges, we used threshold indicator taxa analysis (TITAN) to compare macroinvertebrate assemblage responses to water quality gradients among ecoregions in Mississippi. We identified individual taxa and assemblage-level responses to increasing water quality degradation in MAP streams. Observed responses occurred at higher concentrations for SC, total organic carbon (TOC) and total phosphorus (TP), but not total nitrogen (TN) relative to other ecoregions. These responses appeared to be driven by a large proportion of indicator taxa considered tolerant or unresponsive in other ecoregions, responding negatively to increasing water quality stressors in MAP streams. Our observed assemblage-level stressor responses to WQ gradients in MAP streams demonstrate shifting tolerance in highly altered ecosystems may require adjustments to recovery expectations but also provide useful measures for monitoring improvements in regional water quality. For example, our observed macroinvertebrate assemblage response to increasing TP identified a management goal similar to guidance based on distributional analysis of water quality data within the MAP ecoregion (0.11 vs 0.128 mg L−1) and thus provide some biological basis for previously identified nutrient goals for the region. Our approach can guide and monitor success of nutrient reduction efforts in MAP watersheds and other alluvial plain agroecosystems where reference conditions do not exist, and local and regional taxa pools are less diverse and may not support full recovery of ecological assemblages. While our results are promising, they should also be compared with more sensitive and less habitat-limited biological assemblages (e.g., algae or bacteria) to better understand complex ecological responses to best management practices designed to increase sustainability of high production agricultural regions. more...

Abstract Unilateral pulmonary vein atresia (UPVA) is a rare congenital vascular malformation with obliteration of the pulmonary vein. We present a case series of three siblings with variable presentation of UPVA. We suggest a dominant genetic cause based on different paternity. Identifying genetic etiology would contribute to early diagnosis and screening. more...

BackgroundComprehensive multidisciplinary assessment of neurodevelopmental outcomes of high-risk neonates may have significant challenges in low- and middle-income countries, in addition to socio-cultural barriers. We aimed to compare the time to diagnosis of neurodevelopmental impairment (NDI) and cerebral palsy (CP) in preterm neonates ( more...

Hospital and community healthcare providers have expressed concerns around the continuity and quality of care for infants with neonatal abstinence syndrome (NAS) during hospitalization and transition home. This qualitative study explored the experiences of hospital and community-based healthcare providers and identified themes related to the management of NAS for mothers and infants. Healthcare providers that cared for women with substance use disorders and/or cared for newborns with NAS in a large urban setting in Canada met inclusion criteria for this study and were interviewed in groups or as individuals. Interview transcripts were reviewed iteratively using inductive thematic analysis to identify an overarching theme linked with primary themes. In total, 45 healthcare providers were interviewed. Qualitative analysis of their experiences derived the overarching theme of hope with five primary themes being: mother/infant, mental health, system, judgement, and knowledge. The study identified gaps in NAS care including fear, stigma, and language. This research demonstrates that programs and interventions that work with mothers and newborns with NAS must foster hope in mothers, families, and in the extended care provider team and improve communication between hospital and community networks. more...