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The Ivrea-Verbano Zone (IVZ) is the westernmost sector of the Southern Alps. It is constituted by granulite-to-amphibolite-facies continental crust representing the basement of the Adria plate. The IVZ contains many orogenic mantle peridotites. The largest mantle bodies are aligned along the Insubric Line at the lowest stratigraphic units, in contact with mafic-ultramafic crustal intrusives. Mantle bodies in the central and southern sectors of IVZ are spinel lherzolites with spinel dunites and variable amount of clinopyroxenite, websterite and subordinate anhydrous/hydrous gabbroic bodies (e.g. the Baldissero, Balmuccia, Premosello peridotites). Conversely, modally-metasomatised spinel harzburgites with large dunite bodies and phlogopite-and-amphibole-bearing websterites (e.g. the Finero peridotite) crop out in the northern IVZ.The constant association of the IVZ mantle peridotites with High-T shear zones suggests that none of them was emplaced into the crust by mantle diapirism. Alternative hypotheses involve emplacement at the crustal level at the onset of the Mesozoic extensional regime or tectonic addition to accretionary wedges of Paleozoic subduction zones. Recent gravimetric and seismic data converge in indicating that high-density rocks are very close to the surface near the Insubric Line, thus supporting the possibility that the largest mantle peridotites may be a direct expression of the underlying subcontinental mantle.This contribution focuses on new field, petrographic, geochemical and geochronological data, to address some relevant issues, such as the nature of the spinel lherzolite (refractory residue vs. refertilised mantle), the origin of pyroxenites and gabbros, the relationships with the associated crustal intrusives and the record of Mesozoic tectono-magmatic events.The final goal is to provide new insights into the geodynamic evolution of the mantle bodies and the mantle-crust systems at the Laurasia-Gondwana margin, defining in particular how the mantle heterogeneity acquired during Paleozoic may have governed the rifting process of the Adria margin in Jurassic times.

Dyke swarms intruding the mantle–continental crust transition of the Adria plate as documented by the Ivrea-Verbano Zone (IVZ, Southern Alps) represent a unique opportunity to investigate the evolution of mantle melts from Late Paleozoic to Mesozoic in the post-collisional Variscan realm. Thus, we present new petrological and geochemical data of dyke swarms cropping out in the Finero Phlogopite Peridotite mantle unit. Dykes are from a few cm to >1 m thick and cut at a high angle the mantle foliation.The dyke swarms are composed of cumulus phlogopite-bearing amphibole peridotite, hornblendite, diorite and anorthosite. Many dykes are composite, showing variable proportions of melanocratic and leucocratic layers. Volatiles overpressure during the late magmatic stage is testified by plastic flow and development of a porphyroclastic structure by deformation of early cumulates and by the widespread segregation of a fine-grained mica matrix. The dyke swarms show mineralogical and geochemical features varying between two end-member series.A dyke series is characterized by Al-rich pargasite (Al2O3 up to 18 wt.%) and phlogopite, associated with apatite, calcite, sulphides and sometimes sapphirine. The amphiboles show i) large LILE and LREE contents, ii) negative Nb, Ta, Zr and Hf anomaly and iii) isotopic oxygen composition heavier than the mantle interval, which support the occurrence of recycled continental crust components in the parent melts and impart an overall “orogenic” affinity.The second series mainly consists of Al-poorer pargasite, phlogopite and albite (An 8-10), associated with apatite, monazite, ilmenite, zircon, Nb-rich oxides and carbonates. Mineral compositions and assemblages indicate that the parent melts were strongly enriched in Fe, Na, H2O, P and C. Amphiboles are still enriched in LILE and LREE, but show extreme enrichments in Nb, Ta, Zr and Hf. As a whole, the petrochemical features point to an “anorogenic” alkaline affinity. Zircons from the “anorogenic” dykes are mostly anhedral, with homogenous internal structure or sector zoning. The strongly positive εHft (average of +10) of zircons and the Sr isotopic composition of amphiboles (0.7042) point to a derivation of such “anorogenic” melts from mildly enriched mantle sources. Concordant 206Pb/238U zircon ages for “anorogenic” dykes vary from 221 ± 9 Ma to 192 ± 8 Ma. Some dykes show both “orogenic” and “anorogenic” affinities, thus recording different pulses of mantle melts and metasomatic overprinting. As a whole, the dyke swarms show a transition from “orogenic” to “anorogenic” affinity indicating re-opening of dykes’ conduits for the melt ascending, pointing to a progressive change of the mantle sources of the Mesozoic magmatism of the Southern Alps.

The measurement of residual stresses in exhumed rocks yields valuable information about metamorphic temperature and pressure, deformation and rheology, and stress state. However, the state of elastic strain and stress at the surface of a sample does not necessarily correspond to the state well below the surface. When a sample under elastic strain is cut, polished, or otherwise prepared for analysis, a part of the constraining rock is removed, allowing for the partial relaxation of the elastic strain. To be able to work with residual elastic strain and stress with analytical methods that probe the upper few microns of a sample, the process of strain relaxation must be well understood.For this work we used high-angular resolution EBSD to analyse stressed quartz inclusions in natural garnet from a range of settings, and in several samples grown in piston-cylinder experiments that were previously analysed with Raman spectroscopy for inclusion pressures. The experimental samples are not expected to have undergone plastic deformation in the garnet during cooling, as the majority of the pressure within the inclusion built up during decompression at room temperature. Additionally, the inclusion pressures in buried inclusions matches what is expected for the experimental conditions, suggesting no plastic yielding. Thus, in these samples we can isolate elastic strain from potential plastic deformation. One of the experimental samples was analysed with TEM to test this expectation.Forescatter images reveal topographical effects resembling quartz and adjacent garnet “extruding” out of the sample. Furthermore, rotations of the quartz lattice and the garnet lattice immediately around the quartz inclusion are observed. The rotation axis of the misorientation generally lies in the plane of the sample surface. TEM analysis revealed a number of dislocations in experimental garnet where these were not expected. However, a significant degree of bending of a wedge of garnet between the original sample surface and a quartz inclusion is also observed.The dislocations observed with TEM do not fit with the model of the experiments. Also, the formation of dislocations before sample preparation does not explain the dependence of the rotation axis on the surface orientation. A likely scenario for the deformation measured with EBSD is that the partial relaxation of elastic strains in stressed quartz inclusions in garnet as result of sample preparation induced local distortion of the inclusion and host. Additionally, the persistence of topographical features related to this relaxation despite several steps of polishing suggests that relaxation is not instantaneous but occurs over time.

Whey is the main byproduct of the dairy industry and contains sugars (lactose) and proteins (especially serum proteins and, at lesser extent, residual caseins), which can be valorized by the fermentative action of yeasts. In the present study, we characterized the spoilage yeast population inhabiting natural whey starter (NWS), the undefined starter culture of thermophilic lactic acid bacteria used in Parmigiano Reggiano (PR) cheesemaking, and evaluated thermotolerance, mating type, and the aptitude to produce ethanol and bioactive peptides from whey lactose and proteins, respectively, in a selected pool of strains. PCR-RFLP assay of ribosomal ITS regions and phylogenetic analysis of 26S rDNA D1/D2 domains showed that PR NWS yeast population consists of the well-documented Kluyveromyces marxianus, as well as of other species (Saccharomyces cerevisiae, Wickerhamiella pararugosa, and Torulaspora delbrueckii), with multiple biotypes scored within each species as demonstrated by (GTG)5-based MSP-PCR. Haploid and diploid K. marxianus strains were identified through MAT genotyping, while thermotolerance assay allowed the selection of strains suitable to grow up to 48 °C. In whey fermentation trials, one thermotolerant strain was suitable to release ethanol with a fermentation efficiency of 86.5%, while another candidate was able to produce the highest amounts of both ethanol and bioactive peptides with potentially anti-hypertensive function. The present work demonstrated that PR NWS is a reservoir of ethanol and bioactive peptides producer yeasts, which can be exploited to valorize whey, in agreement with the principles of circularity and sustainability.

The Ivrea-Verbano Zone (IVZ, westernmost sector of the Southern Alps) represents a unique opportunity to investigate the Paleozoic to Mesozoic geodynamic evolution of the Gondwana and Laurasia boundary from the perspective of the lower continental crust. Only recently, the petrochemical record of Triassic-Jurassic magmatism has been recognized. It mainly affected the northernmost tip, the Finero Complex, where the continental crust was tectonically thinned before opening of Alpine Tethys. However, the Mesozoic magmatism in the Finero Complex is still poorly-constrained. Firstly, its extent is largely unknown, because the mantle and crustal intrusives were already enriched by Paleozoic processes. Secondly, Mesozoic melts migration started when the Finero Complex was still placed at P-T conditions typical of a continental crust-mantle transition (1 GPa): this has promoted the reopening of the geochronological clocks in both Paleozoic and Mesozoic rocks, which usually provides wide time intervals. Lastly, the finding of Mesozoic magmatism as composite veins/pods and metasomatised layers has not allowed an exhaustive reconstruction of the primitive melts geochemistry. To place further constraints on such issue, a new dyke swarm cropping out in the Finero Phlogopite Peridotite mantle unit has been investigated. Dykes usually cut at high angle the mantle foliation and are up to 60 cm thick. They are composed by coarse-grained hornblendite to anorthosite, both phlogopite/biotite-bearing. Many dykes are composite, showing variable proportions of hornblendite and anorthosite. In places, the dyke swam was affected by volatiles overpressure as late magmatic stage, which produced plastic flow and development of a porphyroclastic structure by deformation of the early cumulates, with widespread segregation of a fine-grained mica matrix.Dykes mainly consist of pargasite, phlogopite/biotite, albite (An 8-10), in association with apatite, monazite, ilmenite, zircon, Nb-rich oxides, carbonates. Enrichments in Fe (amphibole and biotite) and Na (plagioclase) suggest segregation from evolved melts, strongly enriched in H2O, P, C. The large LILE and LREE contents in amphiboles, sometimes associated to high Nb, Ta, Zr and Hf concentrations, as well as the mineral assemblage, support an alkaline affinity of the melts. The strongly positive εHft (+10) of zircons and the isotopic Sr composition of amphiboles (0.7042) point to a derivation of the melts from mildly enriched sources, possibly located at the crust-mantle interface.Zircons from anorthosite layers are mostly anhedral fragments. They show homogenous internal structure or sector zoning. Concordant 206Pb/238U zircon ages vary from 221 ± 9 Ma to 192 ± 8 Ma. The results of this study confirm that mantle input to the Southern Alps magmatism was of alkaline affinity from Norian to Sinemurian. A widespread fluids circulation induced by such magmatism at high P-T conditions was likely the main cause of the diffuse geochronological reset towards Mesozoic ages of the northern IVZ.

International audience; A detailed microstructural, geochemical and geochronological study was performed on zircon grains from plagioclase-rich dioritic dykes discordantly intruded within meta-diorites/gabbros forming the External Gabbro unit of the Finero Mafic Complex (Italian Alps). This unit is exposed as part of a near complete crustal section spanning from mantle rocks to upper crustal metasediments (Val Cannobina, Ivrea–Verbano Zone, Italy). The leucocratic dykes consist mainly of plagioclase (An18–24Ab79–82Or0.3–0.7) with subordinate amounts of biotite and spinel defining melanocratic layers. Zircon and corundum are common accessory phases. Both the dykes and the surrounding meta-diorites/gabbros show evidence of ductile deformation under amphibolite-facies conditions.Zircon grains/fragments (up to 2 mm in length) occur as porphyroclasts surrounded by fine-grained plagioclase within the leucocratic domains and may occur within the melanocratic layers completely or partially surrounded by biotite and spinels. Fractures are common within zircon, define networks and have associated displacements occasionally and/or they can be filled by secondary minerals.Cathodoluminescence (CL) images reveal that zircon grains from the leucocratic layers show relicts of primary magmatic (i.e. oscillatory and or sector) zoning generally related with the crystal shapes or crystallographic orientation, whereas those from the melanocratic domains do not. In both cases, zircon shows secondary CL features, i.e. mosaic-like textures, due to deformation. EBSD maps confirmed a profuse mosaic texture, resulting in an internal misorientation of ca. 10°, generally associated with fractures. Locally, zircon shows clear evidence of crystal-plastic deformation at the edges, with a gradual misorientations of up to 12°, suggesting an origin prior fragmentation.Trace elements and U–Pb analyses were carried out by LA-ICP-MS directly on petrographic thin sections. Such in situ investigations revealed a strong correlation between internal zircon structures, chemistry, U–Pb isotope ratios and mylonitic fabric.U–Pb data return highly discordant and variable ages. The 206Pb/238U ages may range from ca. 297 to 198 Ma within the same zircon grain from the leucocratic layers, whereas 206Pb/238U younger than 250 Ma were systematically obtained from zircon within the melanocratic layers. The 206Pb/238U data younger than ca. 240 Ma from zircon grains within the leucocratic layers were obtained from narrow axial stripes observed in CL images and oriented parallel or at low-angle with respect to the foliation planes. These stripes are characterized by an overall HREE, Y, U and Th enrichment possibly reflecting deformation of the grain in presence of interstitial fluid phases. Combining U–Pb data, microstructure and zircon CL features, we suggest a multistage evolution of the dykes whereby the melanocratic layers are the result of a Late Permian metasomatic event promoting modification of the pre-existing mineral assemblage of the (Late Carboniferous–Early Permian?) dykes and deformation and partial resetting of zircon porphyroclasts. This has important implications in the geology of the Ivrea–Verbano Zone because, having the dyke intruded the External Gabbro unit before Triassic (probably during Carboniferous–Permian), the latter cannot be considered a Triassic intrusion. At least part of the External Gabbro unit is Carboniferous–Permian as the other mafic bodies of the Ivrea–Verbano Zone.

Case summary A 17-year-old neutered male European Shorthair cat was presented owing to an inability to jump and respiratory stridor. The owner did not report any other clinical signs. On physical examination, the main findings were plantigrade stance, broad facial features and inspiratory stridor. Neurological examination revealed posterior paraparesis, hypotonia and right hindlimb muscle atrophy. Laboratory findings were unremarkable and glycaemia was normal. Serum insulin-like growth factor 1 concentration was elevated (>1000 ng/ml). A total body CT scan showed an enlarged pituitary gland, thickening of the nasal turbinates and an L7–S1 right foraminal stenosis. Electrodiagnostic testing confirmed the presence of a neuropathy affecting both sciatic nerves. The cat was treated with gabapentin only and was still alive and euglycaemic 16 months after the diagnosis. Relevance and novel information This case describes for the first time sciatic neuropathy, an occasional complication of acromegaly in people, as a possible clinical presentation in acromegalic cats without concurrent diabetes mellitus.

The aim of this retrospective, cross-sectional, study was to evaluate clinical findings and outcomes for different ultrasonographic patterns of hepatic emphysema in dogs and cats. Dogs and cats with an ultrasonographic diagnosis of hepatic emphysema and a known outcome, from January 2010 to January 2018, were enrolled. The following data were recorded from medical and ultrasonographic records: ultrasonographic patterns of hepatic emphysema (parenchymal, portal venous, biliary), clinical signs, laboratory findings, and outcomes (favorable, poor). A total of 33 dogs and four cats met the inclusion criteria. Among these, 23 cases were classified as hepatic portal venous gas, 10 as parenchymal emphysema, and four as biliary emphysema. Clinical diagnosis categories were as follows: infection/sepsis (9), gastro-intestinal disease (9), iatrogenic (9), trauma (5), and liver neoplasia (5). An increase in serum liver enzymes was significantly associated with parenchymal emphysema (P = .03). Other clinical and laboratory findings were not associated with the type of hepatic emphysema. Hepatic portal venous gas was mostly transient in patients with ultrasonographic follow-up. The overall mortality was 40.5%. A significant difference was found between mortality by portal venous gas (21.7%) and mortality by parenchymal emphysema (90%) (P = .003). In conclusion, the ultrasonographic differentiation of hepatic emphysema between hepatic portal venous gas and parenchymal emphysema may be important for the prognosis of hepatic emphysema. The presence of parenchymal emphysema may be a poor prognostic indicator, while hepatic portal venous gas may be more benign. However, ultrasound findings should be carefully evaluated in the context of clinical findings.

Objective This article describes the sonoelastographic features of the patellar ligament of sound dogs and tests feasibility, reproducibility and repeatability. Methods Clinically healthy medium-to-large breed dogs were enrolled. Sonoelastographic images of the patellar ligaments were obtained in lateral recumbency with the stifle flexed by an experienced operator and by a senior veterinary student. The elasticity colour map included red (soft), green (intermediate) and blue (hard). Tissue elasticity was measured by calculating the percentage of softness with dedicated software. Categorical, qualitative data analysis was performed using a weighted kappa statistic for repeatability and reproducibility. A categorical qualitative assessment was performed based on a grading scale of 1 to 5 (soft, mostly soft, intermediate, mostly hard and hard). Results Fourteen clinically normal dogs were considered. A total of 28 patellar ligaments were examined. Overall, 25 of the patellar ligaments were graded as soft or mostly soft and the remaining 3 as intermediate. Repeatability was 86.2%, with a weighted kappa of 0.64 (good), for the well-trained sonographer and 83.3%, with a weighted kappa of 0.53 (moderate), for the senior student. Reproducibility was 86.2%, with a weighed kappa of 0.65 (good). Clinical Significance Sonoelastography of the canine patellar ligament is a feasible and reproducible technique. Patellar ligaments in clinically normal dogs showed highly elastic biomechanical properties.

The ultrasonographic findings of kidneys, liver and urinary bladder of 288 Persian and 44 Exotic Shorthair clinically normal cats that underwent screening for polycystic kidney disease (PKD) between July 2003 and December 2005 were reviewed. Cats were divided into two groups, one including cats aged

The aim of this study was to describe the ultrasonographic features of grass awns in soft tissue. A 10 MHz linear transducer was used. Ultrasound images from 25 dogs (27 awns) were collected and compared with the results from water bath studies using wild oat seeds (Avena spp.) collected in the field. Wild oat seeds were the most common grass awn found in soft tissue of dogs. Ultrasonographically grass awns appeared as a double/triple spindle-shaped echogenic interface within soft tissue. The same appearance was observed in water bath studies. In four dogs, the grass awn was removed surgically with a clamp introduced into a fistulous tract, using sonographic guidance. The grass awn was not found surgically in only three dogs, suggesting more attention during surgery. Ultrasonography is a useful diagnostic imaging technique to identify grass awns within soft tissue.

SummaryObjectives: To evaluate how the inclination and torsional deformity of the hemipelvis using extra-rotation as a model affect acetabular angle (AA) and dorsal acetabular rim angle (DARA) assessment with computed tomography (CT).Methods: A normal canine hemipelvis positioned in dorsal recumbency was scanned with a 16-slice multidetector CT scanner, with different degrees of torsional deformity, using extra-rotation around the long axis (0–5–10–15°) of the hemipelvis. Each degree of extra-rotation was acquired at 0°, +20° and –20° of gantry tilt, to mimic different pelvic inclinations on its transverse axis. Cranial and central individual acetabular angles (IAA) and central DARA were calculated and correlated with inclination and torsional deformity.Results: A very strong negative correlation was found between cranial and central IAA, pelvic inclination, and torsional deformity. A very strong positive correlation was found between DARA, pelvic inclination, and torsional deformity.Clinical significance: Pelvic inclination and torsional deformity affect acetabular angles assessment with CT. The greater the inclination (ilia far from the tabletop in dorsal recumbency) and torsional deformity of the pelvis, the worse the acetabulum appeared. A standardized scanning protocol for acetabular morphology assessment is needed and it should consider pelvic inclination. The actual relevance of pelvic torsional deformity is not well known and it should be investigated more thoroughly.

The purpose of this paper is to characterize the sonographic and radiographic features of hemipenes in male snakes and to use these features for gender determination. The sonographic and radiographic examination of 17 male and 10 female snakes, whose gender was determined previously by probing, were evaluated by one examiner who was unaware of the gender. Sonographically, hemipenes appeared funnel shaped with an echogenic thin wall and hypo to anechoic contents. Radiographically, hemipenes filled by positive contrast medium appeared as funnel-shaped radiopacities pointing caudally. Snake gender was correctly determined in all snakes using sonography (accuracy = 100%). Radiographic accuracy was 81.4%. Interpretation of radiographic examinations was limited by poor distribution of the contrast medium within the hemipenes.

Autosomal-dominant polycystic kidney disease (AD-PKD) is common in Persians and Persians-related breeds. The aims of this study were to evaluate the sensitivity and specificity of early ultrasound examination and to compare ultrasound and genetic testing for early diagnosis. Sixty-three Persians and seven Exotic Shorthairs were considered. All underwent ultrasonographic and genetic testing (polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) assay) between 2.5 and 3.5 months of age (10–14 weeks). With ultrasound, 41.4% showed renal cysts, while 37.1% were PKD positive by genetic testing and DNA sequencing. Six cats with at least one renal cyst were negative by genetic testing, while only one cat negative at ultrasound resulted positive at genetic test. DNA sequencing of three polycystic cats, negative by genetic test, revealed they were heterozygous for the mutation. Agreement was described by Cohen's kappa that resulted 0.85, considering genetic test and DNA sequencing. Sensitivity and specificity of ultrasound were 96.2% and 91%, respectively. Sensitivity was higher and specificity lower than reported previously. The higher sensitivity could be due to improved technical capabilities of ultrasound machines and transducers. Other causes of PKD could explain the lower specificity. In conclusion, ultrasound resulted in a reliable diagnostic method for feline AD-PKD1 at early age and it should always be used with genetic testing, in order to reach a complete screening programme and eventually to identify other genetic mutations.

The purpose of this study was to evaluate the ultrasonographic features of canine lipomas. A group of 94 dogs with a soft tissue mass was considered. All dogs were examined ultrasonographically and cyto/histologic examination was performed. Twenty-four dogs, whose mass was diagnosed as a lipoma, were selected. Fifty-five lipomas were present on the 24 dogs. Fifty-one lipomas were superficial and located subcutaneously. Four lipomas were deep and located in the paratesticular region. Ultrasonographically, lipomas appeared as oval, well-defined, encapsulated, striated masses.