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265 results on '"Mazzarino M"'

13. Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte

Author

Granata, M., Skarmoutsou, E., Mazzarino, M. C., Libra, M., and D'Amico, F.

Subjects
Keratinocytes, Tissue Fixation, Tissue Embedding, COP9 Signalosome Complex, Biopsy, Intracellular Signaling Peptides and Proteins, Immunohistochemistry, S100 Calcium Binding Protein A7, Cell Line, Case-Control Studies, Humans, Psoriasis, Immunocytochemistry, Keratinocyte, S100 proteins, CRISPR-Cas Systems, Peptide Hydrolases
Abstract

The application of immunohistological methods provides an invaluable contribution in revealing the protein colocalization, which may reflect the occurrence of molecular interaction processes.This chapter describes comprehensive protocols for detection of S100A7/JAB1 colocalization by immunohistochemistry in archival formalin-fixed and paraffin-embedded skin biopsies from healthy and psoriatic subjects. In addition, we provide a protocol for immunocytochemical detection of S100A7/JAB1 colocalization in S100A7 CRISPR-activated human keratinocyte cell line.

Published
2019

19. Doping control container for urine stabilization: a pilot study

Author

Tsivou, M. Giannadaki, E. Hooghe, F. Roels, K. Van Gansbeke, W. Garribba, F. Lyris, E. Deventer, K. Mazzarino, M. Donati, F. Georgakopoulos, D.G. Van Eenoo, P. Georgakopoulos, C.G. de la Torre, X. Botrè, F.

Abstract

Urine collection containers used in the doping control collection procedure do not provide a protective environment for urine, against degradation by microorganisms and proteolytic enzymes. An in-house chemical stabilization mixture was developed to tackle urine degradation problems encountered in human sport samples, in cases of microbial contamination or proteolytic activity. The mixture consists of antimicrobial substances and protease inhibitors for the simultaneous inactivation of a wide range of proteolytic enzymes. It has already been tested in lab-scale, as part of World Anti-Doping Agency's (WADA) funded research project, in terms of efficiency against microbial and proteolytic activity. The present work, funded also by WADA, is a follow-up study on the improvement of chemical stabilization mixture composition, application mode and limitation of interferences, using pilot urine collection containers, spray-coated in their internal surface with the chemical stabilization mixture. Urine in plastic stabilized collection containers have been gone through various incubation cycles to test for stabilization efficiency and analytical matrix interferences by three WADA accredited Laboratories (Athens, Ghent, and Rome). The spray-coated chemical stabilization mixture was tested against microorganism elimination and steroid glucuronide degradation, as well as enzymatic breakdown of proteins, such as intact hCG, recombinant erythropoietin and small peptides (GHRPs, ipamorelin), induced by proteolytic enzymes. Potential analytical interferences, observed in the presence of spray-coated chemical stabilization mixture, were recorded using routine screening procedures. The results of the current study support the application of the spray-coated plastic urine container, in the doping control collection procedure. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

Published
2017

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