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14 results on '"Mbambo, Gillian"'

2. An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples.

Author

Adams, Matthew, Joshi, Sudhaunshu N, Mbambo, Gillian, Mu, Amy Z, Roemmich, Shay M, Shrestha, Biraj, Strauss, Kathy A, Johnson, Nicole Eddington, Oo, Khine Zaw, Hlaing, Tin Maung, Han, Zay Yar, Han, Kay Thwe, Thura, Si, Richards, Adam K, Huang, Fang, Nyunt, Myaing M, and Plowe, Christopher V

Subjects
Blood, Humans, Plasmodium falciparum, Plasmodium vivax, Malaria, Falciparum, Malaria, Vivax, DNA, Protozoan, DNA, Ribosomal, RNA, Protozoan, RNA, Ribosomal, 18S, Sensitivity and Specificity, Reverse Transcriptase Polymerase Chain Reaction, Myanmar, Asymptomatic Infections, Malaria, Malaria elimination, RT-PCR, Limits of detection, Nucleic acid, Falciparum, Vivax, DNA, Protozoan, Ribosomal, RNA, 18S, Tropical Medicine, Microbiology, Medical Microbiology, Public Health and Health Services
Abstract

BackgroundHighly sensitive, scalable diagnostic methods are needed to guide malaria elimination interventions. While traditional microscopy and rapid diagnostic tests (RDTs) are suitable for the diagnosis of symptomatic malaria infection, more sensitive tests are needed to screen for low-density, asymptomatic infections that are targeted by interventions aiming to eliminate the entire reservoir of malaria infection in humans.MethodsA reverse transcription polymerase chain reaction (RT- PCR) was developed for multiplexed detection of the 18S ribosomal RNA gene and ribosomal RNA of Plasmodium falciparum and Plasmodium vivax. Simulated field samples stored for 14 days with sample preservation buffer were used to assess the analytical sensitivity and specificity. Additionally, 1750 field samples from Southeastern Myanmar were tested both by RDT and ultrasensitive RT-PCR.ResultsLimits of detection (LoD) were determined under simulated field conditions. When 0.3 mL blood samples were stored for 14 days at 28 °C and 80% humidity, the LoD was less than 16 parasites/mL for P. falciparum and 19.7 copies/µL for P. vivax (using a plasmid surrogate), about 10,000-fold lower than RDTs. Of the 1739 samples successfully evaluated by both ultrasensitive RT-PCR and RDT, only two were RDT positive while 24 were positive for P. falciparum, 108 were positive for P. vivax, and 127 were positive for either P. vivax and/or P. falciparum using ultrasensitive RT-PCR.ConclusionsThis ultrasensitive RT-PCR method is a robust, field-tested screening method that is vastly more sensitive than RDTs. Further optimization may result in a truly scalable tool suitable for widespread surveillance of low-level asymptomatic P. falciparum and P. vivax parasitaemia.

Published
2015

3. Immunogenomic profile at baseline predicts host susceptibility to clinical malaria

Author

Mbambo, Gillian, primary, Dwivedi, Ankit, additional, Ifeonu, Olukemi O., additional, Munro, James B., additional, Shrestha, Biraj, additional, Bromley, Robin E., additional, Hodges, Theresa, additional, Adkins, Ricky S., additional, Kouriba, Bourema, additional, Diarra, Issa, additional, Niangaly, Amadou, additional, Kone, Abdoulaye K., additional, Coulibaly, Drissa, additional, Traore, Karim, additional, Dolo, Amagana, additional, Thera, Mahamadou A., additional, Laurens, Matthew B., additional, Doumbo, Ogobara K., additional, Plowe, Christopher V., additional, Berry, Andrea A., additional, Travassos, Mark, additional, Lyke, Kirsten E., additional, and Silva, Joana C., additional

Published
2023
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5. Prevalence of Clinical and Subclinical Plasmodium falciparum and Plasmodium vivax Malaria in Two Remote Rural Communities on the Myanmar–China Border

Author

Huang, Fang, Takala-Harrison, Shannon, Liu, Hui, Xu, Jian-Wei, Yang, Heng-Lin, Adams, Matthew, Shrestha, Biraj, Mbambo, Gillian, Rybock, Demian, Zhou, Shui-Sen, Xia, Zhi-Gui, Zhou, Xiao-Nong, Plowe, Christopher V., and Nyunt, Myaing M.

Subjects
Adult, Aged, 80 and over, Male, Rural Population, China, Adolescent, Diagnostic Tests, Routine, Plasmodium falciparum, Infant, Articles, Myanmar, Middle Aged, Young Adult, Cross-Sectional Studies, Child, Preschool, parasitic diseases, Malaria, Vivax, Prevalence, Humans, Female, Malaria, Falciparum, Child, Plasmodium vivax, Asymptomatic Infections, Aged
Abstract

Malaria infections may be symptomatic, leading to treatment, or “asymptomatic,” typically detected through active surveillance, and not leading to treatment. Malaria elimination may require purging both types of infection. Using detection methods with different sensitivities, we conducted a cross-sectional study in two rural communities located along the border between China’s Yunnan Province and Myanmar’s Shan and Kachin States, to estimate the prevalence of asymptomatic and symptomatic malaria. In Mong Pawk, all infections detected were asymptomatic, and the prevalence of Plasmodium falciparum was 0.3%, 4.3%, 4.0%, and 7.8% by light microscopy, rapid diagnostic test (RDT), conventional polymerase chain reaction (cPCR), and multiplexed real-time PCR (RT-PCR), respectively, and Plasmodium vivax prevalence was 0% by all detection methods. In Laiza, of 385 asymptomatic participants, 2.3%, 4.4%, and 12.2% were positive for P. vivax by microscopy, cPCR, and RT-PCR, respectively, and 2.3% were P. falciparum-positive only by RT-PCR. Of 34 symptomatic participants in Laiza, 32.4% were P. vivax-positive by all detection methods. Factors associated with infection included gender (males higher than females, P = 0.014), and young age group (5–17 age group compared with others, P = 0.0024). Although the sensitivity of microscopy was adequate to detect symptomatic infections, it missed the vast majority (86.5%) of asymptomatic infections. Although molecular detection methods had no advantage over standard microscopy or RDT diagnosis for clinically apparent infections, malaria elimination along the Myanmar–China border will likely require highly sensitive surveillance tools to identify asymptomatic infections and guide targeted screen-and-treat interventions.

Published
2017

7. Frequent malaria illness episodes in two Malawian patients on antiretroviral therapy soon after stopping cotrimoxazole preventive therapy

Author

Nyangulu, Wongani J.S., primary, Mwinjiwa, Edson, additional, Divala, Titus H., additional, Mungwira, Randy G., additional, Nyirenda, Osward, additional, Kanjala, Maxwell, additional, Mbambo, Gillian, additional, Mallewa, Jane, additional, Taylor, Terrie E., additional, Laurens, Matthew B., additional, Laufer, Miriam K., additional, and Van Oosterhout, Joep J., additional

Published
2017
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11. MOESM1 of An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples

Author

Adams, Matthew, Sudhaunshu Joshi, Mbambo, Gillian, Mu, Amy, Roemmich, Shay, Biraj Shrestha, Strauss, Kathy, Johnson, Nicole, Khine Oo, Hlaing, Tin, Zay Han, Han, Kay, Thura, Si, Richards, Adam, Huang, Fang, Myaing Nyunt, and Plowe, Christopher

Subjects
3. Good health
Abstract

Additional file 1. Primers, probes, cycling condition.

12. MOESM1 of Prevalence of molecular markers of sulfadoxineâ pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan

Author

Yaqoob, Adnan, Aamer Khattak, Nadeem, Muhammad, Fatima, Huma, Mbambo, Gillian, Amed Ouattara, Adams, Matthew, Zeeshan, Nadia, and Takala-Harrison, Shannon

Subjects
parasitic diseases, 3. Good health
Abstract

Additional file 1. Frequency distribution of mutations conferring resistance to sulfadoxineâ pyrimethamine in Plasmodium falciparum isolates from Pakistan.

13. MOESM1 of An ultrasensitive reverse transcription polymerase chain reaction assay to detect asymptomatic low-density Plasmodium falciparum and Plasmodium vivax infections in small volume blood samples

Author

Adams, Matthew, Sudhaunshu Joshi, Mbambo, Gillian, Mu, Amy, Roemmich, Shay, Biraj Shrestha, Strauss, Kathy, Johnson, Nicole, Khine Oo, Hlaing, Tin, Zay Han, Han, Kay, Thura, Si, Richards, Adam, Huang, Fang, Myaing Nyunt, and Plowe, Christopher

Subjects
3. Good health
Abstract

Additional file 1. Primers, probes, cycling condition.

14. MOESM1 of Prevalence of molecular markers of sulfadoxineâ pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan

Author

Yaqoob, Adnan, Aamer Khattak, Nadeem, Muhammad, Fatima, Huma, Mbambo, Gillian, Amed Ouattara, Adams, Matthew, Zeeshan, Nadia, and Takala-Harrison, Shannon

Subjects
parasitic diseases, 3. Good health
Abstract

Additional file 1. Frequency distribution of mutations conferring resistance to sulfadoxineâ pyrimethamine in Plasmodium falciparum isolates from Pakistan.

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