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34. A scalable model for EPA and fatty acid production by Phaeodactylum tricornutum

Author

Gu, Wenjia, Kavanagh, John M., and McClure, Dale D.

Subjects
modelling, Histology, photobioreactor, microalgae, Biomedical Engineering, phaeodactylum tricornutum, Bioengineering, EPA, fatty acid, Biotechnology
Abstract

Data availability statement: The data underpinning this publication can be accessed from Brunel University London's data repository, Brunelfigshare here under a CCBY licence: https://doi.org/10.17633/rd.brunel.21197263.v1. Copyright © 2022 Gu, Kavanagh and McClure.. Large-scale photoautotrophic production of microalgae has the potential to provide a sustainable supply of omega-3 fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) for human and animal nutrition. This study presents a kinetic model for the EPA-producing microalga Phaeodactylum tricornutum in photoautotrophic conditions, with light and nitrogen being the growth limiting factors. The model was developed using a dataset obtained from bench-scale (5 L) cultures and was successfully validated against pilot-scale (50 L) cultures. This model is the first to predict the biomass and total fatty acid accumulation along with the EPA concentrations in the biomass and total fatty acid fraction for microalgae. The model was used to develop an optimized repeated-batch strategy; implementation of this led to increases in the biomass and EPA productivities of 50 and 20% respectively. This clearly indicates the potential of the model to be used as a tool in the design, optimization and scale-up of microalgal systems for EPA production. Australian Government Research Training Program Scholarship

Published
2022

37. Understanding gradients in industrial bioreactors

Author

Nadal-Rey, Gisela, McClure, Dale D., Kavanagh, John M., Cornelissen, Sjef, Fletcher, David F., Gernaey, Krist V., Nadal-Rey, Gisela, McClure, Dale D., Kavanagh, John M., Cornelissen, Sjef, Fletcher, David F., and Gernaey, Krist V.

Abstract

Gradients in industrial bioreactors have attracted substantial research attention since exposure to fluctuating environmental conditions has been shown to lead to changes in the metabolome, transcriptome as well as population heterogeneity in industrially relevant microorganisms. Such changes have also been found to impact key process parameters like the yield on substrate and the productivity. Hence, understanding gradients is important from both the academic and industrial perspectives. In this review the causes of gradients are outlined, along with their impact on microbial physiology. Quantifying the impact of gradients requires a detailed understanding of both fluid flow inside industrial equipment and microbial physiology. This review critically examines approaches used to investigate gradients including large-scale experimental work, computational methods and scale-down approaches. Avenues for future work have been highlighted, particularly the need for further coordinated development of both in silico and experimental tools which can be used to further the current understanding of gradients in industrial equipment.

Published
2021

44. Models of the Gut for Analyzing the Impact of Food and Drugs

Author

Fois, Chiara Anna Maria, primary, Le, Thi Yen Loan, additional, Schindeler, Aaron, additional, Naficy, Sina, additional, McClure, Dale David, additional, Read, Mark Norman, additional, Valtchev, Peter, additional, Khademhosseini, Ali, additional, and Dehghani, Fariba, additional

Published
2019
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48. A potential biotechnological process for the sustainable production of vitamin K1.

Author

Tarento, Thomas D. C., McClure, Dale D., Talbot, Andrea M., Regtop, Hubert L., Biffin, John R., Valtchev, Peter, Dehghani, Fariba, and Kavanagh, John M.

Subjects
*VITAMIN K, *ARTERIAL calcification, *CARBON dioxide, *ISOPENTENOIDS, *BIOSYNTHESIS
Abstract

The primary objective of this review is to propose an approach for the biosynthesis of phylloquinone (vitamin K1) based upon its known sources, its role in photosynthesis and its biosynthetic pathway. The chemistry, health benefits, market, and industrial production of vitamin K are also summarized. Vitamin K compounds (K vitamers) are required for the normal function of at least 15 proteins involved in diverse physiological processes such as coagulation, tissue mineralization, inflammation, and neuroprotection. Vitamin K is essential for the prevention of Vitamin K Deficiency Bleeding (VKDB), especially in neonates. Increased vitamin K intake may also reduce the severity and/or risk of bone fracture, arterial calcification, inflammatory diseases, and cognitive decline. Consumers are increasingly favoring natural food and therapeutic products. However, the bulk of vitamin K products employed for both human and animal use are chemically synthesized. Biosynthesis of the menaquinones (vitamin K2) has been extensively researched. However, published research on the biotechnological production of phylloquinone is restricted to a handful of available articles and patents. We have found that microalgae are more suitable than plant cell cultures for the biosynthesis of phylloquinone. Many algae are richer in vitamin K1 than terrestrial plants, and algal cells are easier to manipulate. Vitamin K1 can be efficiently recovered from the biomass using supercritical carbon dioxide extraction. [ABSTRACT FROM AUTHOR]

Published
2019
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49. A potential biotechnological process for the sustainable production of vitamin K1.

Author

Tarento, Thomas D. C., McClure, Dale D., Talbot, Andrea M., Regtop, Hubert L., Biffin, John R., Valtchev, Peter, Dehghani, Fariba, and Kavanagh, John M.

Subjects
VITAMIN K, ARTERIAL calcification, CARBON dioxide, ISOPENTENOIDS, BIOSYNTHESIS
Abstract

The primary objective of this review is to propose an approach for the biosynthesis of phylloquinone (vitamin K1) based upon its known sources, its role in photosynthesis and its biosynthetic pathway. The chemistry, health benefits, market, and industrial production of vitamin K are also summarized. Vitamin K compounds (K vitamers) are required for the normal function of at least 15 proteins involved in diverse physiological processes such as coagulation, tissue mineralization, inflammation, and neuroprotection. Vitamin K is essential for the prevention of Vitamin K Deficiency Bleeding (VKDB), especially in neonates. Increased vitamin K intake may also reduce the severity and/or risk of bone fracture, arterial calcification, inflammatory diseases, and cognitive decline. Consumers are increasingly favoring natural food and therapeutic products. However, the bulk of vitamin K products employed for both human and animal use are chemically synthesized. Biosynthesis of the menaquinones (vitamin K2) has been extensively researched. However, published research on the biotechnological production of phylloquinone is restricted to a handful of available articles and patents. We have found that microalgae are more suitable than plant cell cultures for the biosynthesis of phylloquinone. Many algae are richer in vitamin K1 than terrestrial plants, and algal cells are easier to manipulate. Vitamin K1 can be efficiently recovered from the biomass using supercritical carbon dioxide extraction. [ABSTRACT FROM AUTHOR]

Published
2019
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50. Modelling Bubble Column Bioreactors Using Computational Fluid Dynamics

Author

McClure, Dale David

Abstract

Bubble columns are widely used in the bio-processing industry to perform large scale, aerobic fermentations. For this reason, there is a clear interest in optimising both the design and operation of such reactors. One cost-effective approach is the development of a Computational Fluid Dynamics (CFD) model of the process; the major advantage of this methodology being that it provides detailed information about the flow patterns within the column, knowledge which is difficult to experimentally obtain at an industrial scale. Such data are of particular value as it has been conjectured that poor distribution of nutrients leads to a reduction in the process yield. Hence, the aim of this work was the development of a CFD model capable of accurately describing flow in bubble columns operated in the industrially relevant heterogeneous flow regime (i.e. at superficial velocities greater than 0.1 m/s). In order to develop and validate such a model, it was necessary to obtain experimental data for both an air/water system as well as an air/fermentation media system, the latter being a topic rarely examined in the literature. Hence, a comprehensive experimental program was undertaken at both the bench-top (using a column 0.19 m in diameter and 1 m in height) and pilot-scales (using a column 0.39 m in diameter and 2 m in height). A comprehensive experimental dataset consisting of measurements of the mixing time, overall hold-up, bubble size distribution, as well as profiles of the local hold-up, liquid velocity and gas velocity was generated. Both experimental configurations were modelled using CFD; with the model predictions being in satisfactory agreement with the experimental data at both scales. The development of a predictive model capable of accurately describing the complex mixing patterns in bubble columns (both with and without the presence of surfactants) operating in the heterogeneous flow regime is seen as a key step in the design and optimisation of such systems.

Published
2014

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