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16 results on '"McLinden, Robert"'

1. Magnitude and Breadth of the Neutralizing Antibody Response in the RV144 and Vax003 HIV-1 Vaccine Efficacy Trials

Author

Montefiori, David C., Karnasuta, Chitraporn, Huang, Ying, Ahmed, Hasan, Gilbert, Peter, de Souza, Mark S., McLinden, Robert, Tovanabutra, Sodsai, Laurence-Chenine, Agnes, Sanders-Buell, Eric, Moody, M. Anthony, Bonsignori, Mattia, Ochsenbauer, Christina, Kappes, John, Tang, Haili, Greene, Kelli, Gao, Hongmei, LaBranche, Celia C., Andrews, Charla, Polonis, Victoria R., Rerks-Ngarm, Supachai, Pitisuttithum, Punnee, Nitayaphan, Sorachai, Kaewkungwal, Jaranit, Self, Steve G., Berman, Phillip W., Francis, Donald, Sinangil, Faruk, Lee, Carter, Tartaglia, Jim, Robb, Merlin L., Haynes, Barton F., Michael, Nelson L., and Kim, Jerome H.

Published
2012
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3. Inhibition of HIV replication by sense and antisense Rev response elements in HIV-based retroviral vectors

Author

Kim, Jerome H., McLinden, Robert J., Mosca, Joseph D., Vahey, Maryanne T., Greene, Warner C., and Redfield, Robert R.

Subjects
HIV (Viruses) -- Reproduction, HIV infection -- Research, Health
Abstract

An antisense version of an HIV RNA sequence might be a promising treatment for HIV infection. The RNA sequence is called the Rev response element, or RRE, because the viral Rev protein binds to it. Binding of Rev to RRE is crucial for HIV replication. Researchers showed that both sense and antisense versions of RRE inhibited viral reproduction in an HIV-infected cell line.

Published
1996

4. Transcriptional effects of superinfection in HIV chronically infected T cells: studies in dually infected clones

Author

Kim, Jerome H., McLinden, Robert J., Mosca, Joseph D., Burke, Donald S., Boswell, R. Neal, Birx, Deborah L., and Redfield, Robert R.

Subjects
HIV (Viruses), T cells, Health
Abstract

Some strains of HIV appear to inhibit other strains when both are present in T cells. The infection of a cell by one strain when the cell is already infected with another is called superinfection. Normally, HIV-infected cells resist superinfection, but several researchers have successfully superinfected cell cultures. One group has found that the RF strain of HIV can superinfect cells infected with the LAI strain. When this happens, the RF strain inhibits the LAI strain, possibly because it has a more active Tat protein and because of its integration site on the host DNA.

Published
1996

5. Novel Strategy To Adapt Simian-Human Immunodeficiency Virus E1 Carrying env from an RV144 Volunteer to Rhesus Macaques: Coreceptor Switch and Final Recovery of a Pathogenic Virus with Exclusive R5 Tropism

Author

Scinto, Hanna B., primary, Gupta, Sandeep, additional, Thorat, Swati, additional, Mukhtar, Muhammad M., additional, Griffiths, Anthony, additional, Delgado, Jennifer, additional, Plake, Elizabeth, additional, Vyas, Hemant K., additional, Strickland, Amanda, additional, Byrareddy, Siddappa N., additional, Montefiori, David C., additional, LaBranche, Celia, additional, Pal, Ranajit, additional, Treece, Jim, additional, Orndorff, Sharon, additional, Ferrari, Maria Grazia, additional, Weiss, Deborah, additional, Chenine, Agnes-Laurence, additional, McLinden, Robert, additional, Michael, Nelson, additional, Kim, Jerome H., additional, Robb, Merlin L., additional, Rerks-Ngarm, Supachai, additional, Pitisuttithum, Punnee, additional, Nitayaphan, Sorachai, additional, and Ruprecht, Ruth M., additional

Published
2018
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6. Neutralization Sensitivity of a Novel HIV-1 CRF01_AE Panel of Infectious Molecular Clones

Author

Chenine, Agnes-Laurence, primary, Merbah, Melanie, additional, Wieczorek, Lindsay, additional, Molnar, Sebastian, additional, Mann, Brendan, additional, Lee, Jenica, additional, O'Sullivan, Anne-Marie, additional, Bose, Meera, additional, Sanders-Buell, Eric, additional, Kijak, Gustavo H., additional, Herrera, Carolina, additional, McLinden, Robert, additional, O'Connell, Robert J., additional, Michael, Nelson L., additional, Robb, Merlin L., additional, Kim, Jerome H., additional, Polonis, Victoria R., additional, and Tovanabutra, Sodsai, additional

Published
2018
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7. Identification of New Regions in HIV-1 gp120 Variable 2 and 3 Loops that Bind to α4β7 Integrin Receptor

Author

Peachman, Kristina K., primary, Karasavvas, Nicos, additional, Chenine, Agnes-Laurence, additional, McLinden, Robert, additional, Rerks-Ngarm, Supachai, additional, Jaranit, Kaewkungwal, additional, Nitayaphan, Sorachai, additional, Pitisuttithum, Punnee, additional, Tovanabutra, Sodsai, additional, Zolla-Pazner, Susan, additional, Michael, Nelson L., additional, Kim, Jerome H., additional, Alving, Carl R., additional, and Rao, Mangala, additional

Published
2015
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8. Impact of HIV-1 Backbone on Neutralization Sensitivity: Neutralization Profiles of Heterologous Envelope Glycoproteins Expressed in Native Subtype C and CRF01_AE Backbone

Author

Chenine, Agnès-Laurence, primary, Wieczorek, Lindsay, additional, Sanders-Buell, Eric, additional, Wesberry, Maggie, additional, Towle, Teresa, additional, Pillis, Devin M., additional, Molnar, Sebastian, additional, McLinden, Robert, additional, Edmonds, Tara, additional, Hirsch, Ivan, additional, O’Connell, Robert, additional, McCutchan, Francine E., additional, Montefiori, David C., additional, Ochsenbauer, Christina, additional, Kappes, John C., additional, Kim, Jerome H., additional, Polonis, Victoria R., additional, and Tovanabutra, Sodsai, additional

Published
2013
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9. Detection of HIV-1 Neutralizing Antibodies in a Human CD4+/CXCR4+/CCR5+ T-Lymphoblastoid Cell Assay System

Author

McLinden, Robert J., primary, LaBranche, Celia C., additional, Chenine, Agnès-Laurence, additional, Polonis, Victoria R., additional, Eller, Michael A., additional, Wieczorek, Lindsay, additional, Ochsenbauer, Christina, additional, Kappes, John C., additional, Perfetto, Stephen, additional, Montefiori, David C., additional, Michael, Nelson L., additional, and Kim, Jerome H., additional

Published
2013
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10. 167 Magnitude and Breadth of the Neutralizing Antibody Response in RV144

Author

Karnasuta, Chitraporn, primary, McLinden, Robert, additional, Souza, Mark de, additional, Karasavva, Nicos, additional, Tovanabutra, Sodsai, additional, Laurence-Chenine, Agnes, additional, Sanders-Buell, Eric, additional, Ochsenbauer, Christina, additional, Kappes, John, additional, Polonis, Victoria, additional, Michael, Nelson, additional, Kim, Jerome, additional, and Montefiori, David, additional

Published
2011
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12. Specific Antibody Responses to Vaccination with Bivalent CM235/SF2 gp120: Detection of Homologous and Heterologous Neutralizing Antibody to Subtype E (CRF01.AE) HIV Type 1

Author

Kim, Jerome H., primary, Pitisuttithum, Punnee, additional, Kamboonruang, Chirasak, additional, Chuenchitra, Thippawan, additional, Mascola, John, additional, Frankel, Sarah S., additional, DeSouza, Mark S., additional, Polonis, Victoria, additional, McLinden, Robert, additional, Sambor, Anna, additional, Brown, Arthur E., additional, Phonrat, Benjaluck, additional, Rungruengthanakit, Kittipong, additional, Duliege, Anne-Marie, additional, Robb, Merlin L., additional, McNeil, John, additional, and Birx, Deborah L., additional

Published
2003
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15. Detection of HIV-1 Neutralizing Antibodies in a Human CD4+/CXCR4+/CCR5+ T-Lymphoblastoid Cell Assay System.

Author

McLinden, Robert J., LaBranche, Celia C., Chenine, Agnès-Laurence, Polonis, Victoria R., Eller, Michael A., Wieczorek, Lindsay, Ochsenbauer, Christina, Kappes, John C., Perfetto, Stephen, Montefiori, David C., Michael, Nelson L., and Kim, Jerome H.

Subjects
*IMMUNOGLOBULINS, *CD4 antigen, *CXCR4 receptors, *LYMPHOBLASTOID cell lines, *BIOLOGICAL assay, *ANTIVIRAL agents
Abstract

Sensitive assays are needed to meaningfully assess low levels of neutralizing antibodies (NAbs) that may be important for protection against the acquisition of HIV-1 infection in vaccine recipients. The current assay of choice uses a non-lymphoid cell line (TZM-bl) that may lack sensitivity owing to over expression of CD4 and CCR5. We used transfection of a human CD4+/CXCR4+/α4β7+ T-lymphoblastoid cell line (A3.01) with a CMV IE promoter-driven CCR5neo vector to stably express CCR5. The resulting line, designated A3R5, is permissive to a wide range of CCR5-tropic circulating strains of HIV-1, including HIV-1 molecular clones containing a Tat-inducible Renilla luciferase reporter gene and expressing multiple Env subtypes. Flow cytometric analysis found CCR5 surface expression on A3R5 cells to be markedly less than TZM-bl but similar to CD3.8 stimulated PBMC. More importantly, neutralization mediated by a diverse panel of monoclonal antibodies, HIV-1 positive polyclonal sera and sCD4 was consistently greater in A3R5 compared to TZM-bl cells. The A3R5 cell line provides a novel approach to guide the development and qualification of promising new HIV-1 vaccine immunogens. [ABSTRACT FROM AUTHOR]

Published
2013
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16. Neutralization Sensitivity of a Novel HIV-1 CRF01_AE Panel of Infectious Molecular Clones.

Author

Chenine AL, Merbah M, Wieczorek L, Molnar S, Mann B, Lee J, OʼSullivan AM, Bose M, Sanders-Buell E, Kijak GH, Herrera C, McLinden R, OʼConnell RJ, Michael NL, Robb ML, Kim JH, Polonis VR, and Tovanabutra S

Subjects
Cell Line, HIV-1 immunology, HIV-1 pathogenicity, Humans, Antibodies, Neutralizing immunology, HIV Infections virology, HIV-1 genetics
Abstract

Background: HIV-1 CRF01&#95;AE is dominant in Thailand where RV144 vaccine trial was conducted. To study immune correlates of protection in ongoing trials, CRF01&#95;AE-derived reagents are essential. Here, we present a panel of 14 HIV-1 infectious molecular clones (IMCs) identified from different stages of infection and characterization of their neutralization sensitivity using 2 standard assays., Methods: One full-length IMC was constructed using a transmitted-founder virus to express Renilla luciferase (LucR) reporter gene and full-length envelopes (envs) of exogenous HIV-1. A panel of IMCs was generated, expressing envs of viruses from acute (Fiebig stages I/II and I-IV) and chronic (>Fiebig VI) infection. Neutralization assays were performed using TZM-bl or A3R5 cell lines, and sera or monoclonal antibodies (mAbs). Wilcoxon matched-paired test was used to assess neutralization differences between assays and reagents; correlation coefficients were evaluated by linear regression., Results: Neutralization potency observed was significantly higher in the A3R5 assay when testing mAbs and serum pools (P < 0.0001); the stage of infection from which env was derived did not associate with IMC neutralization sensitivity. Neutralization values from A3R5 and TZM-bl assays were strongly correlated when mAbs were tested (R = 0.7, P < 0.0001), but a weaker association was seen with serum pools (R = 0.17, P = 0.03)., Conclusions: This novel panel of CRF01&#95;AE reporter IMC is useful for assessing vaccine-induced neutralizing antibodies in multiple assays, including those using primary cell targets. The significant differences in TZM-bl and A3R5 neutralization sensitivity, as well as the poor association when using polyclonal sera indicates the need for caution in choosing one specific platform.

Published
2018
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