The methanol extract of the bark of Entada rheedii has been fractionated to afford petroleum ether, dichloromethane, ethyl acetate and aqueous soluble fraction through modified Kupchan partitioning protocol and those fractions has been subjected to repeated-chromatographic separation by Sephadex size exclusion chromatography and purification processes to isolate the secondary metabolites. A total of seven compounds have been isolated from the bark of E. rheedii, which were identified as epicatechin (1), liquiritigenin (2), glabridin (3), 4′-O-methylglabridin (4), isoliquiritigenin (5), hispaglabridin A (6) and shinflavanone (7). This is the first report of isolation of these compounds from E. rheedii.