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Aim: To examine the decreased of metastatic associated protein-1 (MTA-1), complex of cyclin-dependent kinase-2 (CDK2)--cyclin E, and nuclear factor-kappa beta (NF-ĸB) expression as a molecular target therapy of oral Burkitt's lymphoma (Raji) cells mediated by oligonucleotides KIP-1 sense (KIP-1 S) and SKP-2 antisense (SKP-2 AS). Materials and Methods: In the study, the pure laboratory experimental with posttest only control group design was confirmed. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazolium bromide] assay was performed to evaluate the suppression of cell growth. The chemotactic migration activity was carried out by Boyden chamber assay. Activation of MTA-1, NF-ĸB, cyclin E, CDK2, SKP-2, KIP-1, and α-tubulin was investigated by Western blot analysis. Apoptosis cells were analyzed by caspase-3 and caspase-9. Results: The growth inhibition and chemotactic migration activity of KIP-1 S and SKP-2 AS cells were significantly inhibited when compared with the scrambled control (SC) cells. Interestingly, Raji-KIP-1 S has potentially greater cell growth and migrated chemotactic suppression than SKP-2 AS. Induction of cell apoptosis was confirmed in KIP-1 S and SKP-2 AS proofed by increasing the activation of caspase-3 and caspase-9. Decreased level of MTA-1, NF-ĸB, cyclin E, CDK2, SKP-2, and increased level of KIP-1 protein were detected in KIP-1 S- and SKP-2 AS-treated cells. Conclusion: KIP-1 S and SKP-2 AS have strong antitumor activity on the oral Burkitt's lymphoma cells through downregulation of MTA-1, CDK2--cyclin E complex, and NF-kB expression. However, KIP-1 S had a stronger antitumor activity than SKP-2 AS targeting these molecules could represent a promising new therapeutic approach for this type of tumor. [ABSTRACT FROM AUTHOR]

Burkitt's lymphoma (BL) is an uncommon type of Non-Hodgkin lymphoma (NHL). It is a highly aggressive type of B-cell lymphoma and the most common childhood cancer. Treatment for this cancer is still limited. However, a new strategy for refractory tumor, gene therapy is watched with keen interest. The aim of study was to investigate the expression of non-metastatic 23 (nm-23) and metastatic associated protein-1 (MTA-1) of an oral malignant Burkitt's lymphoma induced by oligonucleotide p27 sense (p27 S). The true experimental study with post-test only control group design was confirmed in this study. For detection of nm-23, MTA-1, p27Kip-1 and a- tubulin protein expressions were carried-out by Western blotting analysis. Induction of apoptosis was analyzed by double staining using acridine orange-ethidium bromide (AO-EB). Results revealed the expression of nm-23 and p27Kip-1 protein was markedly increased in cell transfected with p27 sense. Contrally, down regulation of MTA-1 protein was detected in these cell transfectant. Apoptosis induction was significantly elevated in Raji-p27 S compared with that of p27 AS. In conclusion, oligonucleotide p27 S has a strong antitumor activity in oral malignant Burkitt's lymphoma through increasing the apoptosis activity, expression of nm-23 and p27Kip-1 protein induction, and suppressing the expression of MTA-1 protein targeting this molecule could represent a promising new therapeutic approach for this type of cancer. [ABSTRACT FROM AUTHOR]