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394 results on '"Meeley, Robert"'

4. Maize SBP-box transcription factors unbranched2 and unbranched3 affect yield traits by regulating the rate of lateral primordia initiation

Author

Chuck, George S, Brown, Patrick J, Meeley, Robert, and Hake, Sarah

Subjects
Genetics, 2.1 Biological and endogenous factors, Aetiology, Meristem, Mutation, Organogenesis, Plant, Plant Proteins, Quantitative Trait Loci, Transcription Factors, Zea mays, QTL, inflorescence, maize, meristem, yield
Abstract

The separation of male and female flowers in maize provides the potential for independent regulation of traits that affect crop productivity. For example, tassel branch number controls pollen abundance and length of shedding time, whereas ear row number directly affects kernel yield. Mutations in duplicate SBP-box transcription factor genes unbranched2 (ub2) and ub3 affect both of these yield traits. Double mutants display a decrease in tassel branch number and an increase in ear row number, both of which are enhanced by loss of a related gene called tasselsheath4 (tsh4). Furthermore, triple mutants have more tillers and leaves-phenotypes seen in Corngrass1 mutants that result from widespread repression of SBP-box genes. Immunolocalization of UB2 and UB3 proteins revealed accumulation throughout the meristem but absence from the central domain of the meristem where cells regenerate. Thus, ub2, ub3, and tsh4 function as redundant factors that limit the rate of cell differentiation to the lateral domains of meristems. When these genes are mutated, cells are allocated to lateral primordia at a higher rate, causing a net loss of cells from the central domain and premature termination of the inflorescence. The ub3 locus is tightly linked to quantitative trait loci (QTL) for ear row number and tassel branch number in both the nested association mapping (NAM) and intermated B73 by Mo17 (IBM) populations of maize recombinant inbreds, indicating that this gene may be agronomically important. Analysis of ear and tassel QTL across biparental families suggests that multiple mutations in ub3 independently regulate male and female inflorescence development.

Published
2014

5. Parallel tuning of semi‐dwarfism via differential splicing of Brachytic1 in commercial maize and smallholder sorghum

Author

Jiao, Shuping, primary, Mamidi, Sujan, additional, Chamberlin, Mark A., additional, Beatty, Mary, additional, Thatcher, Shawn, additional, Simcox, Kevin D., additional, Maina, Fanna, additional, Wang‐Nan, Hu, additional, Johal, Gurmukh S., additional, Heetland, Lynn, additional, Marla, Sandeep R., additional, Meeley, Robert B., additional, Schmutz, Jeremy, additional, Morris, Geoffrey P., additional, and Multani, Dilbag S., additional

Published
2023
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20. Genetic Perturbation of the Maize Methylome

Author

Li, Qing, Eichten, Steven R., Hermanson, Peter J., Zaunbrecher, Virginia M., Song, Jawon, Wendt, Jennifer, Rosenbaum, Heidi, Madzima, Thelma F., Sloan, Amy E., Huang, Ji, Burgess, Daniel L., Richmond, Todd A., McGinnis, Karen M., Meeley, Robert B., Danilevskaya, Olga N., Vaughn, Matthew W., Kaeppler, Shawn M., Jeddeloh, Jeffrey A., and Springer, Nathan M.

Published
2014

48. A Mutator transposon insertion is associated with ectopic expression of a tandemly repeated multicopy Myb gene pericarp color1 of maize

Author

Robbins, Michael L., Sekhon, Rajandeep S., Meeley, Robert, and Chopra, Surinder

Subjects
Gene expression -- Research, Transposons -- Properties, Allelomorphism -- Identification and classification, Corn -- Genetic aspects, Biological sciences
Abstract

The molecular basis of tissue-specific pigmentation of maize carrying a tandemly repeated multicopy allele of pericarp color1 (p1) was examined using Mutator (Mu) transposon-mediated mutagenesis. The P1-wrallele conditions a white or colorless pericarp and a red cob glumes phenotype. However, a Mu-insertion allele, designated as P1-wr-mum6, displayed an altered phenotype that was first noted as occasional red stripes on pericarp tissue. This gain-of-pericarp-pigmentation phenotype was heritable, yielding families that displayed variable penetrance and expressivity. In one fully penetrant family, deep red pericarp pigmentation was observed. Several reports on Mu suppressible alleles have shown that Mu transposons can affect gene expression by mechanisms that depend on transposase activity. Conversely, the P1-wr-mum6 phenotype is not affected by transposase activity. The increased pigmentation was associated with elevated mRNA expression of P1-wr-mum6 copy (or copies) that was uninterrupted by the transposons. Genomic bisulfite sequencing analysis showed that the elevated expression was associated with hypomethylation of a floral-specific enhancer that is ~4.7 kb upstream of the Mu1 insertion site and may be proximal to an adjacent repeated copy. We propose that the Mu1 insertion interferes with the DNA methylation and related chromatin packaging of P1-wr, thereby inducing expression from gene copy (or copies) that is otherwise suppressed.

Published
2008

49. Functional analysis of maize RAD51 in meiosis and double-strand break repair

Author

Li, Jin, Harper, Lisa C., Golubovskaya, Inna, Wang, C. Rachel, Weber, David, Meeley, Robert B., McElver, John, Bowen, Ben, Cande, W. Zacheus, and Schnable, Patrick S.

Subjects
Corn -- Genetic aspects, Corn -- Physiological aspects, Meiosis -- Evaluation, Genetic recombination -- Evaluation, Amino acids -- Physiological aspects, Amino acids -- Genetic aspects, Biological sciences
Abstract

In Saccharomyces cerevisiae, Rad51p plays a central role in homologous recombination and the repair of double-strand breaks (DSBs). Double mutants of the two Zea mays L. (maize) rad51 homologs are viable and develop well under normal conditions, but are male sterile and have substantially reduced seed set. Light microscopic analyses of male meiosis in these plants reveal reduced homologous pairing, synapsis of nonhomologous chromosomes, reduced bivalents at diakinesis, numerous chromosome breaks at anaphase I, and that >33% of quartets carry cells that either lack an organized nucleolus or have two nucleoli. This indicates that RAD51 is required for efficient chromosome pairing and its absence results in nonhomologous pairing and synapsis. These phenotypes differ from those of an Arabidopsis rad51 mutant that exhibits completely disrupted chromosome pairing and synapsis during meiosis. Unexpectedly, surviving female gametes produced by maize rad51 double mutants are euploid and exhibit near-normal rates of meiotic crossovers. The finding that maize rad51 double mutant embryos are extremely susceptible to radiation-induced DSBs demonstrates a conserved role for RAD51 in the repair of mitotic DSBs in plants, vertebrates, and yeast.

Published
2007

50. The maize premature senesence2 encodes for PHYTOCHROME‐DEPENDENT LATE‐FLOWERING and its expression modulation improves agronomic traits under abiotic stresses

Author

Gupta, Rajeev, primary, Jiao, Shuping, additional, Zhao, Suling, additional, Meeley, Robert B., additional, Williams, Robert W., additional, Taramino, Graziana, additional, Feng, Dongsheng, additional, Li, Guofu, additional, Liu, Juan, additional, Allen, Stephen M., additional, Simcox, Kevin D., additional, and Multani, Dilbag S., additional

Published
2020
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