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5. Metabolic profiles in drought-tolerant wheat with enhanced abscisic acid sensitivity.

Author

Weng, Yuanjie, Mega, Ryosuke, Abe, Fumitaka, Tsujimoto, Hisashi, and Okamoto, Masanori

Subjects
*BRANCHED chain amino acids, *ABSCISIC acid, *DROUGHTS, *PRODUCTION losses, *ARID regions, *WHEAT, *WHEAT breeding
Abstract

Global warming has led to the expansion of arid lands and more frequent droughts, which are the largest cause of global food production losses. In our previous study, we developed TaPYLox wheat overexpressing the plant hormone abscisic acid (ABA) receptor, which is important for the drought stress response in plants. TaPYLox showed resistance to drought stress and acquired water-saving traits that enable efficient grain production with less water use. In this study, we used TaPYLox to identify ABA-dependent and -independent metabolites in response to drought stress. We compared the variation of metabolites in wheat under well-watered, ABA treatment, and drought stress conditions using the ABA-sensitive TaPYLox line and control lines. The results showed that tagatose and L-serine were ABA-dependently regulated metabolites, because their stress-induced accumulation was increased by ABA treatment in TaPYLox. In contrast, L-valine, L-leucine, and DL-isoleucine, which are classified as branched chain amino acids, were not increased by ABA treatment in TaPYLox, suggesting that they are metabolites regulated in an ABA-independent manner. Interestingly, the accumulation of L-valine, L-leucine, and DL-isoleucine was suppressed in drought-tolerant TaPYLox under drought stress, suggesting that drought-tolerant wheat might be low in these amino acids. 3-dehydroshikimic acid and α-ketoglutaric acid were decreased by drought stress in an ABA-independent manner. In this study, we have succeeded in identifying metabolites that are regulated by drought stress in an ABA-dependent and -independent manner. The findings of this study should be useful for future breeding of drought-tolerant wheat. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Aegilops tauschii Introgressions Improve Physio-Biochemical Traits and Metabolite Plasticity in Bread Wheat under Drought Stress

Author

Itam, Michael, Abdelrahman, Mostafa, Yamasaki, Yuji, Mega, Ryosuke, Gorafi, Yasir, Akashi, Kinya, Tsujimoto, Hisashi, Itam, Michael, Abdelrahman, Mostafa, Yamasaki, Yuji, Mega, Ryosuke, Gorafi, Yasir, Akashi, Kinya, and Tsujimoto, Hisashi

Abstract

With the aim of providing genetic materials for breeding drought-resistant wheat varieties, the physiological and metabolic plasticity of three drought-resistant wheat multiple synthetic derivative lines (MSDLs) and their backcross parent “Norin 61” (N61) were evaluated in response to drought stress. The results indicated that the D-genome introgressions from Aegilops tauschii into the MDSLs improved their drought-adaptive traits. Specifically, MNH5 and MSD345 showed higher photosynthesis rates and triose phosphate utilization than N61 under control conditions, resulting in greater accumulation of glucose and sucrose in the shoots. However, under drought stress, MNH5 and MSD345 had higher intrinsic water use efficiency than MSD53 and N61. The total antioxidant capacity and superoxide dismutase activity increased in all three MSDLs, whereas no significant changes were found in N61 in response to drought stress. Metabolome analysis identified six common drought-induced metabolites in all of the investigated genotypes. However, four metabolites (adenine, gamma aminobutyric acid, histidine, and putrescine) each specifically accumulated in an MSDL in response to drought stress, suggesting that these metabolites are important for MSDL drought resistance. In conclusion, MNH5 and MSD345 showed better adaptive responses to drought stress than MSD53 and N61, suggesting that these two MSDLs could be explored for breeding drought-resistant wheat lines.

Published
2022

19. Aegilops tauschii Introgressions Improve Physio-Biochemical Traits and Metabolite Plasticity in Bread Wheat under Drought Stress

Author

Itam, Michael, Abdelrahman, Mostafa, Yamasaki, Yuji, Mega, Ryosuke, Gorafi, Yasir, Akashi, Kinya, Tsujimoto, Hisashi, Itam, Michael, Abdelrahman, Mostafa, Yamasaki, Yuji, Mega, Ryosuke, Gorafi, Yasir, Akashi, Kinya, and Tsujimoto, Hisashi

Abstract

With the aim of providing genetic materials for breeding drought-resistant wheat varieties, the physiological and metabolic plasticity of three drought-resistant wheat multiple synthetic derivative lines (MSDLs) and their backcross parent “Norin 61” (N61) were evaluated in response to drought stress. The results indicated that the D-genome introgressions from Aegilops tauschii into the MDSLs improved their drought-adaptive traits. Specifically, MNH5 and MSD345 showed higher photosynthesis rates and triose phosphate utilization than N61 under control conditions, resulting in greater accumulation of glucose and sucrose in the shoots. However, under drought stress, MNH5 and MSD345 had higher intrinsic water use efficiency than MSD53 and N61. The total antioxidant capacity and superoxide dismutase activity increased in all three MSDLs, whereas no significant changes were found in N61 in response to drought stress. Metabolome analysis identified six common drought-induced metabolites in all of the investigated genotypes. However, four metabolites (adenine, gamma aminobutyric acid, histidine, and putrescine) each specifically accumulated in an MSDL in response to drought stress, suggesting that these metabolites are important for MSDL drought resistance. In conclusion, MNH5 and MSD345 showed better adaptive responses to drought stress than MSD53 and N61, suggesting that these two MSDLs could be explored for breeding drought-resistant wheat lines., identifier:https://doi.org/10.3390/agronomy10101588

Published
2020

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