1. Genome-wide Inference of Somatic Translocation Events During Potato Dihaploid Production
- Author
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Gina M. Pham, Guilherme T. Braz, Megan Conway, Emily Crisovan, John P. Hamilton, F. Parker E. Laimbeer, Norma Manrique-Carpintero, Linsey Newton, David S. Douches, Jiming Jiang, Richard E. Veilleux, and C. Robin Buell
- Subjects
Plant culture ,SB1-1110 ,Genetics ,QH426-470 - Abstract
Potato ( L.) breeders often use dihaploids, which are 2× progeny derived from 4× autotetraploid parents. Dihaploids can be used in diploid crosses to introduce new genetic material into breeding germplasm that can be integrated into tetraploid breeding through the use of unreduced gametes in 4× by 2× crosses. Dihaploid potatoes are usually produced via pollination by haploid inducer lines known as in vitro pollinators (IVP). In vitro pollinator chromosomes are selectively degraded from initially full hybrid embryos, resulting in 2× seed. During this process, somatic translocation of IVP DNA may occur. In this study, a genome-wide approach was used to identify such events and other chromosome-scale abnormalities in a population of 95 dihaploids derived from a cross between potato cultivar Superior and the haploid inducing line IVP101. Most Superior dihaploids showed translocation rates of
- Published
- 2019
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