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4 results on '"Meijuan Miao"'

1. MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia

Author

Hong Zhao, Fei Liu, Ruichun Jia, Huiying Chang, Haixia Li, Meijuan Miao, Hui Wang, and Zhiping Yang

Subjects
Chronic myelogenous leukemia, IRS1, IRS2, miR-570, Medicine
Abstract

Objective(s): Chronic myelogenous leukemia (CML) is a chronic myeloproliferative disorder characterized by the accumulation of myeloid cells with a chromosomal translocation known as the Philadelphia chromosome. In this study, we investigated the roles of miR-570 in CML development. Materials and Methods: Expression of miR-570 in CML samples and cell lines was determined by qRT-PCR. Glucose uptake and ATP concentration detection assays were used to analyze cell glucose metabolism. MTT and western blot assays were performed for cell proliferation and apoptosis, respectively. The targets of miR-570 were predicted by bioinformatics and confirmed using luciferase activity, qRT-PCR and western blot assays. Results: The expression levels of miR-570 were significantly reduced in CML clinical samples and cells. Overexpression of miR-570 inhibited cell proliferation, promoted apoptosis, and suppressed glucose metabolism in CML cells. Insulin receptor substrates (IRS) 1 and IRS2 were identified as direct targets of miR-570. IRS1 or IRS2 were knocked down in K562 cells.Loss of IRS1/2 expression led to suppressed cell proliferation, elevated apoptosis, and decreased glucose metabolism in CML cells, which is consistent with their roles as miR-570 targets. Conclusion: MiR-570 directly targeted IRS1 and IRS2 in CML, suppressing cell proliferation and glucose metabolism. MiR-570 may provide a strategy for CML therapy.

Published
2017
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3. Sodium Selenite Improves The Therapeutic Effect Of BMSCs Via Promoting The Proliferation And Differentiation, Thereby Promoting The Hematopoietic Factors

Author

Meijuan Miao, Lei Zhang, Hui Wang, Xi Chen, Botao Tang, Daoyuan Liu, Guangyi Sui, Lixin Yan, Qi Zhang, and Dongmei Yan

Subjects
0301 basic medicine, proliferation, coagulation factors, OncoTargets and Therapy, sodium selenite, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, medicine, Pharmacology (medical), CD90, clotting time, Original Research, biology, Cell growth, Chemistry, CD44, Mesenchymal stem cell, hemic and immune systems, differentiation, Cell biology, RUNX2, Haematopoiesis, 030104 developmental biology, medicine.anatomical_structure, Oncology, Adipogenesis, 030220 oncology & carcinogenesis, biology.protein, Bone marrow, BMSCs
Abstract

Dongmei Yan,1,* Botao Tang,2,* Lixin Yan,3 Lei Zhang,1 Meijuan Miao,1 Xi Chen,4 Guangyi Sui,5 Qi Zhang,1 Daoyuan Liu,1 Hui Wang1 1Department of Blood Transfusion, The Second Affiliated Hospital of Harbin Medical University, Harbin, People’s Republic of China; 2Department of Cardiology, Heilongjiang Red Cross Hospital, Harbin, People’s Republic of China; 3Department of Laboratory Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, People’s Republic of China; 4Department of Hematology, The Second Affiliated Hospital of Harbin Medical University, Harbin, People’s Republic of China; 5Ethics Committee, The Tumor Hospital Affiliated to Harbin Medical University, Harbin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hui WangDepartment of Blood Transfusion, The Second Affiliated Hospital, Harbin Medical University, Xuefu Road No. 246, Nangang District, Harbin, Heilongjiang Province, People’s Republic of ChinaTel +86-451-86605134Email wanghui@hrbmu.edu.cnPurpose: Sodium selenite (Na2SeO3) has been known to restore the antioxidant capacity of bone marrow mesenchymal stem cells (BMSCs), reduce the production of reactive oxygen species (ROS) in the cells, and promote cell proliferation and inhibit cell apoptosis. However, it is still not clear whether selenium can mediate the differentiation and inhibit the induced hemagglutination of BMSCs. In this study, we attempted to explore the effect of Na2SeO3 on these aspects of BMSCs.Methods: We evaluated the fate of the MSCs isolated from the bone marrow of mice by studying their differentiation and proliferation after treatment with Na2SeO3. We also simultaneously evaluated the coagulation reaction induced by Na2SeO3-treated BMSCs in vitro.Results: While the mice-derived BMSCs expressed CD44, CD73, CD90, and CD105, they did not express CD45. The morphology of the derived cells was homogeneously elongated. These results showed that the isolated cells are indeed BMSCs. We found that 0.1 μM and 1 μM of Na2SeO3 promoted the proliferation and apoptosis of BMSCs, respectively. This showed that Na2SeO3 can be toxic and exert certain side effects on the BMSCs. The results of the osteogenic and adipogenic assay showed that 0.1 μM Na2SeO3 could significantly promote the osteogenic and adipogenic differentiation of BMSCs by upregulating the lipid factors (LPL and PPRAG) and osteogenic factors, RUNX2, COL1, and BGP, in a concentration-dependent manner. Coagulation experiments in animals (mice and rats) revealed that Na2SeO3 can reduce the coagulation time of BMSCs in a concentration-dependent manner, which is related to the high expression of hematopoietic factors (SDF-1α, GM-CSF, IL-7, IL-8, IL-11, and SCF).Conclusion: Na2SeO3 promotes the proliferation and differentiation as well as reduces the coagulation time of BMSCs, and this effect might enhance the therapeutic effect of BMSCs.Keywords: sodium selenite, BMSCs, proliferation, differentiation, coagulation factors, clotting time

Published
2019

4. Protective effects of Schisandrin B on cigarette smoke-induced airway injury in mice through Nrf2 pathway

Author

Fei Liu, Hui Wang, Hong Zhao, Yanhong Liu, Zhiping Yang, Meijuan Miao, Haogang Zhang, Qiushi Wang, and Ruichun Jia

Subjects
0301 basic medicine, Male, Schisandra chinensis, NF-E2-Related Factor 2, Immunology, Anti-Inflammatory Agents, Inflammation, Pharmacology, Lignans, Cigarette Smoking, 03 medical and health sciences, chemistry.chemical_compound, Cyclooctanes, Mice, Western blot, medicine, Immunology and Allergy, Animals, Polycyclic Compounds, Lung, Cells, Cultured, Schisandra, medicine.diagnostic_test, biology, business.industry, NF-kappa B, NF-κB, Glutathione, Pneumonia, respiratory system, biology.organism_classification, Mice, Inbred C57BL, 030104 developmental biology, Bronchoalveolar lavage, medicine.anatomical_structure, chemistry, Cytokines, medicine.symptom, Signal transduction, business, Signal Transduction
Abstract

Schisandrin B (SchB), a dibenzocyclooctadiene derivative isolated from Schisandra chinensis , has been reported to have anti-inflammatory effects. However, the protective effects of SchB on cigarette smoke (CS)-induced lung inflammation remain unclear. This study was to investigate the effects of SchB on CS-induced lung inflammation in mice. The mice were exposed to CS to develop lung inflammation. SchB was given 1 h before CS exposure daily for five consecutive days. The levels of inflammatory mediators TNF-α, IL-1β, and IL-6 in bronchoalveolar lavage fluid (BALF) were measured in this study. SOD, GSH, MPO and MDA contents were also detected. Furthermore, the expression of Nrf-2 and NF-κB were detected by western blot analysis. Histopathological analyses showed that SchB had protective effects against CS-induced lung inflammation. The levels of inflammatory mediators TNF-α, IL-1β, and IL-6 in BALF were also inhibited by SchB. CS-induced MPO activity and MDA content were inhibited by SchB. The levels of SOD and GSH were up-regulated by SchB. SchB significantly inhibited CS-induced NF-κB activation and up-regulated the expression of Nrf2 and HO-1. In conclusion, these data suggest that SchB protects against CS-induced lung inflammation by activating Nrf2 and inhibiting NF-κB signaling pathway.

Published
2017

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