Search

Your search keyword '"Meininghaus, Mark"' showing total 12 results
Start Over Author "Meininghaus, Mark"
12 results on '"Meininghaus, Mark"'

2. Selective optogenetic control of Gq signaling using human Neuropsin

Author

Wagdi, Ahmed, Malan, Daniela, Sathyanarayanan, Udhayabhaskar, Beauchamp, Janosch S., Vogt, Markus, Zipf, David, Beiert, Thomas, Mansuroglu, Berivan, Dusend, Vanessa, Meininghaus, Mark, Schneider, Linn, Kalthof, Bernd, Wiegert, J. Simon, König, Gabriele M., Kostenis, Evi, Patejdl, Robert, Sasse, Philipp, and Bruegmann, Tobias

Published
2022
Full Text
View/download PDF

3. Single‐Shot Solid‐Phase Synthesis of Full‐Length H2 Relaxin Disulfide Surrogates

Author

Zhao, Rui, primary, Shi, Pan, additional, Cui, Ji‐bin, additional, Shi, Chaowei, additional, Wei, Xiao‐Xiong, additional, Luo, Jie, additional, Xia, Zhemin, additional, Shi, Wei‐Wei, additional, Zhou, Yingxin, additional, Tang, Jiahui, additional, Tian, Changlin, additional, Meininghaus, Mark, additional, Bierer, Donald, additional, Shi, Jing, additional, Li, Yi‐Ming, additional, and Liu, Lei, additional

Published
2023
Full Text
View/download PDF

5. Selective optogenetic control of Gq signaling using human Neuropsin.

Author

Wagdi, Ahmed, Malan, Daniela, Sathyanarayanan, Udhayabhaskar, Beauchamp, Janosch S., Vogt, Markus, Zipf, David, Beiert, Thomas, Mansuroglu, Berivan, Dusend, Vanessa, Meininghaus, Mark, Schneider, Linn, Kalthof, Bernd, Wiegert, J. Simon, König, Gabriele M., Kostenis, Evi, Patejdl, Robert, Sasse, Philipp, and Bruegmann, Tobias

Subjects
CALCIUM channels, TRANSGENIC animals, G proteins, HIGH throughput screening (Drug development), CELLULAR signal transduction, SMALL intestine
Abstract

Gq proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of Gq signaling in vitro and in vivo with high spatio-temporal resolution. Properties and G protein specificity of hOPN5 are characterized by UV light induced IP3 generation, Ca2+ transients and inhibition of GIRK channel activity in HEK cells. In adult hearts from a transgenic animal model, light increases the spontaneous beating rate. In addition, we demonstrate light induced contractions in the small intestine, which are not detectable after pharmacological Gq protein block. All-optical high-throughput screening for TRPC6 inhibitors is more specific and sensitive than conventional pharmacological screening. Thus, we demonstrate specific Gq signaling of hOPN5 and unveil its potential for optogenetic applications. Gq proteins are one of four major classes of G proteins; optogenetic receptors for selective and repetitive activation of Gq proteins with fast kinetics are lacking. Here the authors report UV light-dependent Gq signalling using human Neuropsin (hOPN5) and demonstrate its potential as an optogenetic tool. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

6. Potent and Selective Human Prostaglandin F (FP) Receptor Antagonist (BAY-6672) for the Treatment of Idiopathic Pulmonary Fibrosis (IPF)

Author

Beck, Hartmut, primary, Thaler, Tobias, additional, Meibom, Daniel, additional, Meininghaus, Mark, additional, Jörißen, Hannah, additional, Dietz, Lisa, additional, Terjung, Carsten, additional, Bairlein, Michaela, additional, von Bühler, Clemens-Jeremias, additional, Anlauf, Sonja, additional, Fürstner, Chantal, additional, Stellfeld, Timo, additional, Schneider, Dirk, additional, Gericke, Kersten M., additional, Buyck, Thomas, additional, Lovis, Kai, additional, Münster, Uwe, additional, Anlahr, Johanna, additional, Kersten, Elisabeth, additional, Levilain, Guillaume, additional, Marossek, Virginia, additional, and Kast, Raimund, additional

Published
2020
Full Text
View/download PDF

8. Author Correction: Selective optogenetic control of Gq signaling using human Neuropsin.

Author

Wagdi, Ahmed, Malan, Daniela, Sathyanarayanan, Udhayabhaskar, Beauchamp, Janosch S., Vogt, Markus, Zipf, David, Beiert, Thomas, Mansuroglu, Berivan, Dusend, Vanessa, Meininghaus, Mark, Schneider, Linn, Kalthof, Bernd, Wiegert, J. Simon, König, Gabriele M., Kostenis, Evi, Patejdl, Robert, Sasse, Philipp, and Bruegmann, Tobias

Subjects
TRANSGENIC mice, EXPERIMENTAL medicine, HUMAN beings
Abstract

Correction to: I Nature Communications i https://doi.org/10.1038/s41467-022-29265-w, published online 01 April 2022 The original version of this Article omitted a contributor, Michael Hesse, in the Acknowledgements section. These authors contributed equally: Ahmed Wagdi, Daniela Malan, Udhayabhaskar Sathyanarayanan. [Extracted from the article]

Published
2023
Full Text
View/download PDF

9. Pluripotent Cell-Derived Cardiomyocytes: One Cell Type as a Predictive Tool For the Assessment of Drug Efficacy and Safety on a Variety of Cardiac Targets

Author

Kettenhofen, Ralf F., primary, Tressat, Kristina, additional, Rubenchik, Olga, additional, Stoelzle, Sonja, additional, Yu, Ming, additional, Sonnenhol, Eike, additional, Meininghaus, Mark, additional, Kalthof, Bernd, additional, Bohlen, Heribert, additional, Schwengberg, Silke, additional, and Kolossov, Eugen, additional

Published
2012
Full Text
View/download PDF

12. Requirement of the carboxy-terminal domain of RNA polymerase II for the transcriptional activation of chromosomal c-fosand hsp70A genes

Author

Meininghaus, Mark and Eick, Dirk

Abstract

The carboxy-terminal domain of the large subunit of mouse and human RNA polymerase II contains 52 repeats of a heptapeptide which are the targets for a variety of kinases. We have used an α-amanitin resistant form of the large subunit of pol II to study the role of the carboxy-terminal domain in the expression of chromosomal genes. The large subunit of RNA polymerase II and deletion mutants thereof, which contain only 31 (LSΔ31) and 5 (LSΔ5) repeats, were expressed in 293 cells. Subsequently, the endogenous large subunit of RNA polymerase II was inhibited by α-amanitin and the induction of chromosomal c- fosand hsp70A genes was determined. Cells expressing the large subunit of RNA polymerase II and LSΔ31 were able to transcribe the c- fosand hsp70A genes after treatment with the phorbolester TPA and after heat-shock, respectively. In contrast, cells expressing LSΔ5 failed to induce expression of both genes.

Published
1999
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results