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348 results on '"Melchers, Willem J.G."'

5. Clinical performance of high-risk HPV testing on self-samples versus clinician samples in routine primary HPV screening in the Netherlands: An observational study

Author

Inturrisi, Federica, Aitken, Clare A., Melchers, Willem J.G., van den Brule, Adriaan J.C., Molijn, Anco, Hinrichs, John W.J., Niesters, Hubert G.M., Siebers, Albert G., Schuurman, Rob, Heideman, Daniëlle A.M., de Kok, Inge M.C.M., Bekkers, Ruud L.M., van Kemenade, Folkert J., and Berkhof, Johannes

Published
2021
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7. The one health problem of azole resistance in Aspergillus fumigatus: current insights and future research agenda

Author

Verweij, Paul E., Lucas, John A., Arendrup, Maiken C., Bowyer, Paul, Brinkmann, Arjen J.F., Denning, David W., Dyer, Paul S., Fisher, Matthew C., Geenen, Petra L., Gisi, Ulrich, Hermann, Dietrich, Hoogendijk, Andre, Kiers, Eric, Lagrou, Katrien, Melchers, Willem J.G., Rhodes, Johanna, Rietveld, Anton G., Schoustra, Sijmen E., Stenzel, Klaus, Zwaan, Bas J., and Fraaije, Bart A.

Published
2020
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12. Paradoxal Trends in Azole-Resistant Aspergillus fumigatus in a National Multicenter Surveillance Program, the Netherlands, 2013-2018

Author

Lestrade, Pieter P.A., BuiI, Jochem B., van der Beek, Martha T., Kujper, Ed.J., van Djk, Karin, Kampinga, Greetje A., Rijnders, Bart J.A., Vonk, Alieke G., de Greeff, Sabine C., Schoffelen, Annelot F., van Dissel, Jaap, Meis, Jacques F., Melchers, Willem J.G., and Verwej, Paul E.

Subjects
Ibrutinib, Posaconazole, Health
Abstract

Aspergillus fumigatus is a saprobic mold that thrives on decaying plant material. The fungus is thermotolerant and exhibits optimum growth at 37[degrees]C. A. fumigatus has evolved into an major cause [...]

Published
2020
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13. High quality of SARS-CoV-2 molecular diagnostics in a diverse laboratory landscape through supported benchmark testing and External Quality Assessment

Author

Sluimer, John, van den Akker, Willem M.R., Goderski, Gabriel, Swart, Arno, van der Veer, Bas, Cremer, Jeroen, Chung, Ngoc Hoa, Molenkamp, Richard, Voermans, Jolanda, Guldemeester, Judith, van der Eijk, Annemiek, de Jong, Menno D., Mithoe, Glen, Hermans, Mirjam H.A., de Beer, Jessica L., Wessels, Els, von Wintersdorff, Christian, Pas, Suzan, Verweij, Jaco J., Melchers, Willem J.G., van de Bovenkamp, Jeroen H.B., Vahidnia, Ali, Gard, Lilli, Schuurman, Rob, Wintermans, Bas, Leversteijn-van Hall, Maurine, Smits, Paul, de Groot, Theun, Deiman, Birgit A.L.M., Bart, Aldert, van der Reijden, Wil, Svraka-Latifovic, Sanela, van der Zanden, Adri G.M., Thijsen, Steven, Schubbert, Rainer, Dreesens, Lisa L., van Duijn, Gert, Ong, David S.Y., Oostra, Monique, Bruisten, Sylvia, van Trijp, Marijke, Pettersson, Annika, van Burgel, Nathalie D., Oudbier, Joke, van der Linden, Michael, Rossen, John, Smit, Pieter, Thai, Khoa, Eggink, Dirk, Meijer, Adam, Sluimer, John, van den Akker, Willem M.R., Goderski, Gabriel, Swart, Arno, van der Veer, Bas, Cremer, Jeroen, Chung, Ngoc Hoa, Molenkamp, Richard, Voermans, Jolanda, Guldemeester, Judith, van der Eijk, Annemiek, de Jong, Menno D., Mithoe, Glen, Hermans, Mirjam H.A., de Beer, Jessica L., Wessels, Els, von Wintersdorff, Christian, Pas, Suzan, Verweij, Jaco J., Melchers, Willem J.G., van de Bovenkamp, Jeroen H.B., Vahidnia, Ali, Gard, Lilli, Schuurman, Rob, Wintermans, Bas, Leversteijn-van Hall, Maurine, Smits, Paul, de Groot, Theun, Deiman, Birgit A.L.M., Bart, Aldert, van der Reijden, Wil, Svraka-Latifovic, Sanela, van der Zanden, Adri G.M., Thijsen, Steven, Schubbert, Rainer, Dreesens, Lisa L., van Duijn, Gert, Ong, David S.Y., Oostra, Monique, Bruisten, Sylvia, van Trijp, Marijke, Pettersson, Annika, van Burgel, Nathalie D., Oudbier, Joke, van der Linden, Michael, Rossen, John, Smit, Pieter, Thai, Khoa, Eggink, Dirk, and Meijer, Adam

Abstract

A two-step strategy combining assisted benchmark testing (entry controls) and External Quality Assessments (EQAs) with blinded simulated clinical specimens to enhance and maintain the quality of nucleic acid amplification testing was developed. This strategy was successfully applied to 71 diagnostic laboratories in The Netherlands when upscaling the national diagnostic capacity during the SARS-CoV-2 pandemic. The availability of benchmark testing in combination with advice for improvement substantially enhanced the quality of the laboratory testing procedures for SARS-CoV-2 detection. The three subsequent EQA rounds demonstrated high quality testing with regard to specificity (99.6% correctly identified) and sensitivity (93.3% correctly identified). Even with the implementation of novel assays, changing workflows using diverse equipment and a high degree of assay heterogeneity, the overall high quality was maintained using this two-step strategy. We show that in contrast to the limited value of Cq value for absolute proxies of viral load, these Cq values can, in combination with metadata on strategies and techniques, provide valuable information for laboratories to improve their procedures. In conclusion, our two-step strategy (preparation phase followed by a series of EQAs) is a rapid and flexible system capable of scaling, improving, and maintaining high quality diagnostics even in a rapidly evolving (e.g. pandemic) situation.

Published
2024

19. Defining hrHPV genotypes in cervical intraepithelial neoplasia by laser capture microdissection supports reflex triage of self-samples using HPV16/18 and FAM19A4/miR124-2 methylation

Author

Leeman, Annemiek, Ebisch, Renée M.F., Kasius, Annemieke, Bosgraaf, Remko P., Jenkins, David, van de Sandt, Miekel M., de Strooper, Lise M.A., Heideman, Daniëlle A.M., Snijders, Peter J.F., Massuger, Leon F.A.G., Bekkers, Ruud L.M., Meijer, Chris J.L.M., van Kemenade, Folkert J., Quint, Wim G.V., and Melchers, Willem J.G.

Published
2018
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22. Environmental Hotspots for Azole Resistance Selection of Aspergillus fumigatus, the Netherlands

Author

Schoustra, Sijmen E., Debets, Alfons J.M., Rijs, Antonius J.M.M., Zhang, Jianhua, Snelders, Eveline, Leendertse, Peter C., Melchers, Willem J.G., Rietveld, Anton G., Zwaan, Bas J., and Verweij, Paul E.

Subjects
Posaconazole -- Health aspects, Health
Abstract

A spergillusfumigatus is a saprophytic mold whose natural habitat is decaying plant material (1). This fungus can tolerate high temperatures (>50 [degrees]C) that can occur in heaps of decaying plant [...]

Published
2019
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23. Mycology

Author

Buil, Jochem B., Zoll, Jan, Verweij, Paul E., Melchers, Willem J.G., Bergmans, A.M.C., van Pelt-Verkuil, E., editor, van Leeuwen, W.B., editor, and te Witt, R., editor

Published
2017
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27. Detection of High-grade Prostate Cancer Using a Urinary Molecular Biomarker–Based Risk Score

Author

Van Neste, Leander, Hendriks, Rianne J., Dijkstra, Siebren, Trooskens, Geert, Cornel, Erik B., Jannink, Sander A., de Jong, Hans, Hessels, Daphne, Smit, Frank P., Melchers, Willem J.G., Leyten, Gisèle H.J.M., de Reijke, Theo M., Vergunst, Henk, Kil, Paul, Knipscheer, Ben C., Hulsbergen-van de Kaa, Christina A., Mulders, Peter F.A., van Oort, Inge M., Van Criekinge, Wim, and Schalken, Jack A.

Published
2016
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28. Validation of the FAM19A4/mir124-2 DNA methylation test for both lavage- and brush-based self-samples to detect cervical (pre)cancer in HPV-positive women

Author

De Strooper, Lise M.A., Verhoef, Viola M.J., Berkhof, Johannes, Hesselink, Albertus T., de Bruin, Helena M.E., van Kemenade, Folkert J., Bosgraaf, Remko P., Bekkers, Ruud L.M., Massuger, Leon F.A.G., Melchers, Willem J.G., Steenbergen, Renske D.M., Snijders, Peter J.F., Meijer, Chris J.L.M., and Heideman, Daniëlle A.M.

Published
2016
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30. Aerosol Transmission of Aspergillus fumigates in Cystic Fibrosis Patients in the Netherlands

Author

Engel, Tobias G.P., Erren, Ellen, Driessche, Koen S.J. Vanden, Melchers, Willem J.G., Reijers, Monique H., Merkus, Peter, and Verweij, Paul E.

Subjects
Cystic fibrosis, Molds (Fungi), Bacteria, Fibrosis, Lung diseases, Microorganisms, Aerosols, Health
Abstract

Progressive lung injury in cystic fibrosis (CF) patients can lead to chronic colonization with bacteria and fungi (1,2). Different routes of patient-to-patient transmission have been identified for various microorganisms (3). [...]

Published
2019
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32. Acceptability of risk-based triage in cervical cancer screening:A focus group study

Author

Bas, Sharell, Sijben, Jasmijn, Bischoff, Erik W.M.A., Bekkers, Ruud L.M., de Kok, Inge M.C.M., Melchers, Willem J.G., Siebers, Albert G., van der Waal, Daniëlle, Broeders, Mireille J.M., Bas, Sharell, Sijben, Jasmijn, Bischoff, Erik W.M.A., Bekkers, Ruud L.M., de Kok, Inge M.C.M., Melchers, Willem J.G., Siebers, Albert G., van der Waal, Daniëlle, and Broeders, Mireille J.M.

Abstract

BACKGROUND: Compared to the previous cytology-based program, the introduction of primary high-risk human papillomavirus (hrHPV) based screening in 2017 has led to an increased number of referrals. To counter this, triage of hrHPV-positive women in cervical cancer screening can potentially be optimized by taking sociodemographic and lifestyle risk factors for cervical abnormalities into account. Therefore, it is essential to gain knowledge of the views of women (30-60 years) eligible for cervical cancer screening. OBJECTIVE: The main goal of this qualitative study was to gain insight in the aspects that influence acceptability of risk-based triage in cervical cancer screening. DESIGN: A focus group study in which participants were recruited via four general medical practices, and purposive sampling was used to maximize heterogeneity with regards to age, education level, and cervical cancer screening experiences. APPROACH: The focus group discussions were transcribed verbatim and analyzed using reflexive thematic analysis. PARTICIPANTS: A total of 28 women (average age: 45.2 years) eligible for cervical cancer screening in The Netherlands participated in seven online focus group discussions. Half of the participants was higher educated, and the participants differed in previous cervical cancer screening participation and screening result. KEY RESULTS: In total, 5 main themes and 17 subthemes were identified that determine the acceptability of risk-stratified triage. The main themes are: 1) adequacy of the screening program: an evidence-based program that is able to minimize cancer incidence and reduce unnecessary referrals; 2) personal information (e.g., sensitive topics and stigma); 3) emotional impact: fear and reassurance; 4) communication (e.g., transparency); and 5) autonomy (e.g., prevention). CONCLUSION: The current stud

Published
2023

33. Evaluation of DNA methylation biomarkers ASCL1 and LHX8 on HPV-positive self-collected samples from primary HPV-based screening

Author

Verhoef, Lisanne, Bleeker, Maaike C.G., Polman, Nicole, Steenbergen, Renske D.M., Ebisch, Renée M.F., Melchers, Willem J.G., Bekkers, Ruud L.M., Molijn, Anco C., Quint, Wim G., van Kemenade, Folkert, Meijer, Chris J.L.M., Berkhof, Johannes, Heideman, Daniëlle A.M., Verhoef, Lisanne, Bleeker, Maaike C.G., Polman, Nicole, Steenbergen, Renske D.M., Ebisch, Renée M.F., Melchers, Willem J.G., Bekkers, Ruud L.M., Molijn, Anco C., Quint, Wim G., van Kemenade, Folkert, Meijer, Chris J.L.M., Berkhof, Johannes, and Heideman, Daniëlle A.M.

Abstract

Background: Host-cell DNA methylation analysis can be used to triage women with high-risk human papillomavirus (HPV)-positive self-collected cervicovaginal samples, but current data are restricted to under-/never-screened women and referral populations. This study evaluated triage performance in women who were offered primary HPV self-sampling for cervical cancer screening. Methods: Self-collected samples from 593 HPV-positive women who participated in a primary HPV self-sampling trial (IMPROVE study; NTR5078), were tested for the DNA methylation markers ASCL1 and LHX8 using quantitative multiplex methylation-specific PCR (qMSP). The diagnostic performance for CIN3 and cervical cancer (CIN3 +) was evaluated and compared with that of paired HPV-positive clinician-collected cervical samples. Results: Significantly higher methylation levels were found in HPV-positive self-collected samples of women with CIN3 + than control women with no evidence of disease (P values <0.0001). The marker panel ASCL1/LHX8 yielded a sensitivity for CIN3 + detection of 73.3% (63/86; 95% CI 63.9–82.6%), with a corresponding specificity of 61.1% (310/507; 95% CI 56.9–65.4%). The relative sensitivity for detecting CIN3+ was 0.95 (95% CI 0.82–1.10) for self-collection versus clinician-collection, and the relative specificity was 0.82 (95% CI 0.75–0.90). Conclusions: The ASCL1/LHX8 methylation marker panel constitutes a feasible direct triage method for the detection of CIN3 + in HPV-positive women participating in routine screening by self-sampling.

Published
2023

34. Sociodemographic Characteristics and Screening Outcomes of Women Preferring Self-Sampling in the Dutch Cervical Cancer Screening Programme:A Population-Based Study

Author

Aitken, Clare A., Inturrisi, Federica, Kaljouw, Sylvia, Nieboer, Daan, Siebers, Albert G., Melchers, Willem J.G., van den Brule, Adriaan J.C., Molijn, Anco, Hinrichs, John W.J., Niesters, Hubert G.M., van Kemenade, Folkert J., Berkhof, Johannes, de Kok, Inge M.C.M., Aitken, Clare A., Inturrisi, Federica, Kaljouw, Sylvia, Nieboer, Daan, Siebers, Albert G., Melchers, Willem J.G., van den Brule, Adriaan J.C., Molijn, Anco, Hinrichs, John W.J., Niesters, Hubert G.M., van Kemenade, Folkert J., Berkhof, Johannes, and de Kok, Inge M.C.M.

Abstract

BACKGROUND: In the Netherlands, lower high-risk human papillomavirus (hrHPV) positivity but higher cervical intraepithelial neoplasia (CIN) 2+ detection were found in self-collected compared with clinician-collected samples. To investigate the possible reason for these differences, we compared sociodemographic and screening characteristics of women and related these to screening outcomes. METHODS: We extracted data from PALGA on all primary hrHPV screens and associated follow-up tests for 857,866 screened women, invited in 2017 and 2018. We linked these data with sociodemographic data from Statistics Netherlands. Logistic regression was performed for hrHPV positivity and CIN 2+/3+ detection. RESULTS: Out of the 857,866 women, 6.8% chose to use a self-sampling device. A higher proportion of self-sampling users was ages 30 to 35 years, was not previously screened, was living in a one-person household, or was the breadwinner in the household. After adjustment for these factors self-sampling had lower hrHPV positivity (aOR, 0.65; 95% CI, 0.63-0.68)) as compared with clinician-collected sampling, as well as lower odds of CIN 2+ (aOR, 0.76; 95% CI, 0.70-0.82) and CIN 3+ (aOR, 0.86; 95% CI, 0.78-0.95) detection. CONCLUSIONS: It is likely that the observed differences between the two sampling methods are not only related to sociodemographic differences, but related to differences in screening test accuracy and/or background risk. IMPACT: Self-sampling can be used for targeting underscreened women, as a more convenient screening tool. Further investigation is required to evaluate how to implement self-sampling, when it is used as a primary instrument in routine screening. See related commentary by Arbyn et al., p. 159.

Published
2023

35. Supplementary Methods from Identification and Validation of a 3-Gene Methylation Classifier for HPV-Based Cervical Screening on Self-Samples

Author

Verlaat, Wina, primary, Snoek, Barbara C., primary, Heideman, Daniëlle A.M., primary, Wilting, Saskia M., primary, Snijders, Peter J.F., primary, Novianti, Putri W., primary, van Splunter, Annina P., primary, Peeters, Carel F.W., primary, van Trommel, Nienke E., primary, Massuger, Leon F.A.G., primary, Bekkers, Ruud L.M., primary, Melchers, Willem J.G., primary, van Kemenade, Folkert J., primary, Berkhof, Johannes, primary, van de Wiel, Mark A., primary, Meijer, Chris J.L.M., primary, and Steenbergen, Renske D.M., primary

Published
2023
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36. Supplementary Figures and Tables from Identification and Validation of a 3-Gene Methylation Classifier for HPV-Based Cervical Screening on Self-Samples

Author

Verlaat, Wina, primary, Snoek, Barbara C., primary, Heideman, Daniëlle A.M., primary, Wilting, Saskia M., primary, Snijders, Peter J.F., primary, Novianti, Putri W., primary, van Splunter, Annina P., primary, Peeters, Carel F.W., primary, van Trommel, Nienke E., primary, Massuger, Leon F.A.G., primary, Bekkers, Ruud L.M., primary, Melchers, Willem J.G., primary, van Kemenade, Folkert J., primary, Berkhof, Johannes, primary, van de Wiel, Mark A., primary, Meijer, Chris J.L.M., primary, and Steenbergen, Renske D.M., primary

Published
2023
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43. Trends in Azole Resistance in Aspergillus fumigatus, the Netherlands, 1994-2016

Author

Buil, Jochem B., Snelders, Eveline, Denardi, Laura Bedin, Melchers, Willem J.G., and Verweij, Paul E.

Subjects
Aspergillus -- Health aspects, Microbial drug resistance -- Testing, Azoles -- Testing, Medical centers, Universities and colleges, Health
Abstract

Azole resistance is increasingly reported in Aspergillus fumigatus and is now found all around the world (1). Most studies have investigated the presence of resistance in environmental or clinical samples [...]

Published
2019
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45. Sociodemographic Characteristics and Screening Outcomes of Women Preferring Self-Sampling in the Dutch Cervical Cancer Screening Programme: A Population-Based Study

Author

Aitken, Clare A., primary, Inturrisi, Federica, additional, Kaljouw, Sylvia, additional, Nieboer, Daan, additional, Siebers, Albert G., additional, Melchers, Willem J.G., additional, van den Brule, Adriaan J.C., additional, Molijn, Anco, additional, Hinrichs, John W.J., additional, Niesters, Hubert G.M., additional, van Kemenade, Folkert J., additional, Berkhof, Johannes, additional, and de Kok, Inge M.C.M., additional

Published
2022
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49. Performance of DNA methylation analysis of ASCL1, LHX8, ST6GALNAC5, GHSR, ZIC1 and SST for the triage of HPV-positive women:Results from a Dutch primary HPV-based screening cohort

Author

Verhoef, Lisanne, Bleeker, Maaike C.G., Polman, Nicole, Steenbergen, Renske D.M., Meijer, Chris J.L.M., Melchers, Willem J.G., Bekkers, Ruud L., Molijn, Anco C., Quint, Wim G., van Kemenade, Folkert J., Berkhof, Johannes, Heideman, Daniëlle A.M., Pathology, AII - Cancer immunology, CCA - Cancer Treatment and quality of life, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers, General practice, Epidemiology and Data Science, and APH - Methodology

Subjects
SDG 3 - Good Health and Well-being
Abstract

Methylation of host-cell deoxyribonucleic acid (DNA) has been proposed as a promising biomarker for triage of high-risk (hr) human papillomavirus (HPV) positive women at screening. Our study aims to validate recently identified host-cell DNA methylation markers for triage in an hrHPV-positive cohort derived from primary HPV-based cervical screening in The Netherlands. Methylation markers ASCL1, LHX8, ST6GALNAC5, GHSR, ZIC1 and SST were evaluated relative to the ACTB reference gene by multiplex quantitative methylation-specific PCR (qMSP) in clinician-collected cervical samples (n = 715) from hrHPV-positive women (age 29-60 years), who were enrolled in the Dutch IMPROVE screening trial (NTR5078). Primary clinical end-point was cervical intraepithelial neoplasia grade 3 (CIN3) or cancer (CIN3+). The single-marker and bi-marker methylation classifiers developed for CIN3 detection in a previous series of hrHPV-positive clinician-collected cervical samples were applied. The diagnostic accuracy was visualised using receiver operating characteristic (ROC) curves and assessed through area under the ROC curve (AUC). The performance of the methylation markers to detect CIN3+ was determined using the predefined threshold calibrated at 70% clinical specificity. Individual methylation makers showed good performance for CIN3+ detection, with highest AUC for ASCL1 (0.844) and LHX8 (0.830). Combined as a bi-marker panel with predefined threshold, ASCL1/LHX8 yielded a CIN3+ sensitivity of 76.9% (70/91; 95% CI 68.3-85.6%) at a specificity of 74.5% (465/624; 95% CI 71.1-77.9%). In conclusion, our study shows that the individual host-cell DNA methylation classifiers and the bi-marker panel ASCL1/LHX8 have clinical utility for the detection of CIN3+ in hrHPV-positive women invited for routine screening.

Published
2022
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