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35 results on '"Melendez, Matias E."'

13. Colorimetric Detection of SARS-CoV-2 Using Plasmonic Biosensors and Smartphones

Author

Materón, Elsa M., primary, Gómez, Faustino R., additional, Almeida, Mariana B., additional, Shimizu, Flavio M., additional, Wong, Ademar, additional, Teodoro, Kelcilene B. R., additional, Silva, Filipe S. R., additional, Lima, Manoel J. A., additional, Angelim, Monara Kaelle S. C., additional, Melendez, Matias E., additional, Porras, Nelson, additional, Vieira, Pedro M., additional, Correa, Daniel S., additional, Carrilho, Emanuel, additional, Oliveira, Osvaldo N., additional, Azevedo, Ricardo B., additional, and Goncalves, Débora, additional

Published
2022
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14. Low-cost bacterial nanocellulose-based interdigitated biosensor to detect the p53 cancer biomarker

Author

Bondancia, Thalita J., primary, Soares, Andrey Coatrini, additional, Popolin-Neto, Mário, additional, Gomes, Nathalia O., additional, Raymundo-Pereira, Paulo A., additional, Barud, Hernane S., additional, Machado, Sergio A.S., additional, Ribeiro, Sidney J.L., additional, Melendez, Matias E., additional, Carvalho, André L., additional, Reis, Rui M., additional, Paulovich, Fernando V., additional, and Oliveira, Osvaldo N., additional

Published
2022
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18. Whole‐exome sequencing of non‐ BRCA1/BRCA2 mutation carrier cases at high‐risk for hereditary breast/ovarian cancer

Author

Felicio, Paula S., primary, Grasel, Rebeca S., additional, Campacci, Natalia, additional, Paula, Andre E., additional, Galvão, Henrique C. R., additional, Torrezan, Giovana T., additional, Sabato, Cristina S., additional, Fernandes, Gabriela C., additional, Souza, Cristiano P., additional, Michelli, Rodrigo D., additional, Andrade, Carlos E., additional, Barros, Bruna Durães De Figueiredo, additional, Matsushita, Marcus M., additional, Revil, Timothée, additional, Ragoussis, Jiannis, additional, Couch, Fergus J., additional, Hart, Steven N., additional, Reis, Rui M., additional, Melendez, Matias E., additional, Tonin, Patricia N., additional, Carraro, Dirce M., additional, and Palmero, Edenir I., additional

Published
2020
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19. Influence of the Molecular Orientation and Ionization of Self-Assembled Monolayers in Biosensors: Application to Genosensors of Prostate Cancer Antigen 3

Author

Raymundo-Pereira, Paulo A., primary, de Oliveira Pedro, Rafael, additional, Carr, Olivia, additional, Melendez, Matias E., additional, Gobbi, Angelo L., additional, Helena de Oliveira Piazzetta, Maria, additional, Carvalho, André L., additional, Reis, Rui M., additional, Miranda, Paulo B., additional, and Oliveira, Osvaldo N., additional

Published
2020
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20. Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9

Author

Ibáñez-Redín, Gisela, primary, Materon, Elsa M., additional, Furuta, Roberto H. M., additional, Wilson, Deivy, additional, do Nascimento, Gustavo Freitas, additional, Melendez, Matias E., additional, Carvalho, André L., additional, Reis, Rui Manuel, additional, Oliveira, Osvaldo N., additional, and Gonçalves, Débora, additional

Published
2020
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22. Whole‐exome sequencing of non‐BRCA1/BRCA2 mutation carrier cases at high‐risk for hereditary breast/ovarian cancer.

Author

Felicio, Paula S., Grasel, Rebeca S., Campacci, Natalia, Paula, Andre E., Galvão, Henrique C. R., Torrezan, Giovana T., Sabato, Cristina S., Fernandes, Gabriela C., Souza, Cristiano P., Michelli, Rodrigo D., Andrade, Carlos E., Barros, Bruna Durães De Figueiredo, Matsushita, Marcus M., Revil, Timothée, Ragoussis, Jiannis, Couch, Fergus J., Hart, Steven N., Reis, Rui M., Melendez, Matias E., and Tonin, Patricia N.

Abstract

The current study aimed to identify new breast and/or ovarian cancer predisposition genes. For that, whole‐exome sequencing (WES) was performed in the germline DNA of 52 non‐BRCA1/BRCA2/TP53 mutation carrier women at high‐risk for hereditary breast and ovarian cancer (HBOC). All variants were classified using information from population and disease specific databases, in silico prediction tools and the American College of Medical Genetics and Genomics (ACMG) criteria. Loss of heterozygosity (LOH) of tumor samples and segregation analyses were performed whenever possible. The variants identified were investigated in a second, independent cohort of 17 BC cases. Pathogenic/Likely Pathogenic variants were identified in known cancer genes such as CHEK2, MUTYH, PMS2, and RAD51C. Rare and potentially pathogenic variants were identified in DNA repair genes (FAN1, POLQ, and RAD54L) and other cancer‐related genes such as DROSHA and SLC34A2. Interestingly, the variant c.149T>G in the FAN1 gene was identified in two unrelated families, and exhibited LOH in the tumor tissue of one of them. In conclusion, this is the largest Brazilian WES study involving families at high‐risk for HBOC which has brought novel insights into the role of potentially new genetic risk factors for hereditary breast and ovarian cancer. [ABSTRACT FROM AUTHOR]

Published
2021
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23. Determination of p53 biomarker using an electrochemical immunoassay based on layer-by-layer films with NiFe2O4 nanoparticles.

Author

Ibáñez-Redín, Gisela, Joshi, Nirav, do Nascimento, Gustavo Freitas, Wilson, Deivy, Melendez, Matias E., Carvalho, André L., Reis, Rui Manuel, Gonçalves, Débora, and Oliveira, Osvaldo N. Jr

Abstract

A disposable electrochemical immunosensors is presented suitable to detect cancer biomarker p53 using screen-printed carbon electrodes modified with a layer-by-layer (LbL) matrix of carboxylated NiFe2O4 nanoparticles and polyethyleneimine, onto which anti-p53 antibodies were adsorbed. Under optimized conditions, the immunosensors exhibited high surface coverage and high concentration of immobilized antibodies, which allowed for detection of p53 in a wide dynamic range from 1.0 to 10 × 103 pg mL−1, with a limit of detection of 5.0 fg mL−1 at a working potential of 100 mV vs. Ag/AgCl. The immunosensors also exhibited good selectivity with negligible interference upon incubation in complex matrices containing high concentrations of proteins (i.e., fetal bovine serum and cell lysate). The immunosensor performance is among the best reported in the literature for determination of p53, with the additional advantage of being disposable and operating with low-volume solutions. Graphical abstract [ABSTRACT FROM AUTHOR]

Published
2020
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24. Low-Cost and Rapid-Production Microfluidic Electrochemical Double-Layer Capacitors for Fast and Sensitive Breast Cancer Diagnosis

Author

de Oliveira, Ricardo A. G., primary, Nicoliche, Caroline Y. N., additional, Pasqualeti, Anielli M., additional, Shimizu, Flavio M., additional, Ribeiro, Iris R., additional, Melendez, Matias E., additional, Carvalho, André L., additional, Gobbi, Angelo L., additional, Faria, Ronaldo C., additional, and Lima, Renato S., additional

Published
2018
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25. Hexane partition from Annona crassiflora Mart. promotes cytotoxity and apoptosis on human cervical cancer cell lines

Author

Silva, Viviane A. O., primary, Alves, Ana Laura V., additional, Rosa, Marcela N., additional, Silva, Larissa R. V., additional, Melendez, Matias E., additional, Cury, Fernanda P., additional, Gomes, Izabela N. F., additional, Tansini, Aline, additional, Longato, Giovanna B., additional, Martinho, Olga, additional, Oliveira, Bruno G., additional, Pinto, Fernanda E., additional, Romão, Wanderson, additional, Ribeiro, Rosy I. M. A., additional, and Reis, Rui M., additional

Published
2018
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26. Identification of hereditary cancer in the general population: development and validation of a screening questionnaire for obtaining the family history of cancer

Author

Campacci, Natalia, primary, de Lima, Juliana O., additional, Carvalho, André L., additional, Michelli, Rodrigo D., additional, Haikel, Rafael, additional, Mauad, Edmundo, additional, Viana, Danilo V., additional, Melendez, Matias E., additional, Vazquez, Fabiana de L., additional, Zanardo, Cleyton, additional, Reis, Rui M., additional, Rossi, Benedito M., additional, and Palmero, Edenir I., additional

Published
2017
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27. Immunosensor for Pancreatic Cancer Based on Electrospun Nanofibers Coated with Carbon Nanotubes or Gold Nanoparticles

Author

Soares, Juliana C., primary, Iwaki, Leonardo E. O., additional, Soares, Andrey C., additional, Rodrigues, Valquiria C., additional, Melendez, Matias E., additional, Fregnani, José Humberto T. G., additional, Reis, Rui M., additional, Carvalho, Andre L., additional, Corrêa, Daniel S., additional, and Oliveira, Osvaldo N., additional

Published
2017
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29. Influence of the Molecular Orientation and Ionization of Self-Assembled Monolayers in Biosensors: Application to Genosensors of Prostate Cancer Antigen 3

Author

Raymundo-Pereira, Paulo A., de Oliveira Pedro, Rafael, Carr, Olivia, Melendez, Matias E., Gobbi, Angelo L., Helena de Oliveira Piazzetta, Maria, Carvalho, André L., Reis, Rui M., Miranda, Paulo B., and Oliveira, Osvaldo N.

Abstract

Electrochemical sensing depends on the immobilization of biorecognition elements and charge transfer to the electrode, which can be tuned and sometimes controlled using self-assembled monolayers (SAMs) as matrices. In this study, we show that an efficient immobilization of an ssDNA probe to detect the prostate cancer antigen 3 biomarker is achieved even for less organized SAMs provided that the terminal groups were ionized. These conclusions were reached by comparing the biosensors made with 11-mercaptoundecanoic acid (11-MUA) prepared in ethanol and thioglycolic acid (mercaptoacetic acid: MAA) prepared in ethanolic and aqueous solvents. The highest sensitivity with electrochemical impedance spectroscopy was observed for sensors assembled over MAA monolayers on gold prepared in ethanol and water (at pH 12.5), with limits of detection of 1.2 × 10–9and 1.7 × 10–9mol L–1, respectively. The biosensors made on MAA films prepared in water at pH 7.0 and 2.5 were not efficient because they are mostly terminated by protonated carboxylic acid groups, according to sum-frequency generation vibrational and polarization modulation-infrared reflection-absorption (PM-IRRAS) spectroscopies, which prevent an adequate attachment of the biomarker. Well-organized SAMs of 11-MUA also showed good detection performance, but the limit of detection was not as low, 2.1 × 10–9mol L–1, probably due to its long chains that decrease the charge transfer. Therefore, the matrix fabrication method can control the molecular assembly of biosensors and hence their performance.

Published
2021
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30. Low-cost bacterial nanocellulose-based interdigitated biosensor to detect the p53 cancer biomarker

Author

Bondancia, Thalita J., Soares, Andrey Coatrini, Popolin-Neto, Mário, Gomes, Nathalia O., Raymundo-Pereira, Paulo A., Barud, Hernane S., Machado, Sergio A.S., Ribeiro, Sidney J.L., Melendez, Matias E., Carvalho, André L., Reis, Rui M., Paulovich, Fernando V., and Oliveira, Osvaldo N.

Abstract

Low-cost sensors to detect cancer biomarkers with high sensitivity and selectivity are essential for early diagnosis. Herein, an immunosensor was developed to detect the cancer biomarker p53 antigen in MCF7 lysates using electrical impedance spectroscopy. Interdigitated electrodes were screen printed on bacterial nanocellulose substrates, then coated with a matrix of layer-by-layer films of chitosan and chondroitin sulfate onto which a layer of anti-p53 antibodies was adsorbed. The immunosensing performance was optimized with a 3-bilayer matrix, with detection of p53 in MCF7 cell lysates at concentrations between 0.01 and 1000 Ucell. mL−1, and detection limit of 0.16 UcellmL−1. The effective buildup of the immunosensor on bacterial nanocellulose was confirmed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and surface energy analysis. In spite of the high sensitivity, full selectivity with distinction of the p53-containing cell lysates and possible interferents required treating the data with a supervised machine learning approach based on decision trees. This allowed the creation of a multidimensional calibration space with 11 dimensions (frequencies used to generate decision tree rules), with which the classification of the p53-containing samples can be explained.

Published
2024
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31. Disposable Microfluidic Immunoarray Device for Sensitive Breast Cancer Biomarker Detection

Author

de Oliveira, Ricardo A. G., Materon, Elsa M., Melendez, Matias E., Carvalho, André L., and Faria, Ronaldo C.

Abstract

Breast cancer is the most common cancer in women worldwide. The detection of biomarkers has played a significant role in the early diagnosis and prognosis of breast cancer. Herein, we describe the construction of a disposable microfluidic immunoarray device (DμID) for the rapid and low-cost detection of CA15-3 (carbohydrate antigen 15-3), a protein biomarker for breast cancer. The DμID was constructed using a simple and rapid prototyping technique and was applied to detect CA15-3 in cancer patients. The DμID construction was based on the use of a double-sided adhesive card with a microfluidic channel and a screen-printed array with 8 electrodes. Both the immunoarray and microfluidic channel were designed using an inexpensive home cutter printer and using low-cost materials. The immunoarray was modified using the layer-by-layer technique aiming at immobilizing the primary antibody. For the biomarker detection, magnetic particles (MPs) modified with polyclonal antibodies and peroxidase enzymes were used as a strategy for capture, separation, and preconcentration of the biomarker, in addition to amplification of the electroanalytical signal. The preconcentration and amplification strategies integrated with the nanostructured immunosensors of the DμID meaningfully contributed toward the detection of CA15-3 with a limit of detection (LoD) of 6 μU mL–1, requiring as low as 2 μL of serum samples for 8 simultaneous detections. The obtained LoD was 1200 times lower compared to those of other immunosensors previously reported in the literature. The DμID was applied for the detection of CA15-3 in real samples of breast cancer patients and was found to present an excellent correlation with the well-established commercial electrochemiluminescence immunoassay. The association of the DμID with nanostructured surfaces and analyte capturing with bioconjugated paramagnetic particles is essentially a promising breakthrough for the low-cost and accurate detection of cancer biomarkers.

Published
2024
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35. Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors.

Author

Melendez ME, Fraefel C, and Epstein AL

Subjects
Animals, Cell Line, Chlorocebus aethiops, Gene Transfer Techniques, Humans, Transfection, Transgenes, Vero Cells, Genetic Vectors, Herpesvirus 1, Human genetics, Molecular Biology methods
Abstract

Amplicons are defective, helper-dependent, herpes simplex virus type 1 (HSV-1)-derived vectors. The main interest of these vectors as gene transfer tools stems from the fact that the amplicon vector genomes do not carry protein-encoding viral sequences. Consequently, they are completely safe for the host and nontoxic for the infected cells. Moreover, the complete absence of virus genes provides space to accommodate very large foreign DNA sequences, up to almost 150-kb, the size of the virus genome. This large transgene capacity can be used to deliver complete gene loci, including introns and exons, as well as long regulatory sequences, conferring tissue-specific expression or stable maintenance of the transgene in proliferating cells. During many years the development of these vectors and their application in gene transfer experiments was hindered by the presence of contaminating toxic helper virus particles in the vector stocks. In recent years, however, two different methodologies have been developed that allow generating amplicon stocks either completely free of helper particles or only faintly contaminated with fully defective helper particles. This chapter describes these two methodologies.

Published
2014
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