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30 results on '"Melle, Giovanni"'

1. Development of a Novel Platform for in vitro Electrophysiological Recording

Author

Melle, Giovanni

Subjects
Physics - Biological Physics
Abstract

This PhD thesis introduces the concept of meta-electrodes coupled with laser optoporation for high quality intracellular signals from hiPSCs-CM. These signals can be recorded on high-density commercial CMOS-MEAs from 3Brain characterized by thousands of electrode covered by a thin film of porous Platinum without any rework of the devices, 3D nanostructures or circuitry for electroporation. Subsequently, I attempted to translate these unique features of low invasiveness and reliability to other commercial MEA platforms, in order to develop a new tool for cardiac electrophysiological accurate recordings.

Published
2022

2. Hyperbolic Metamaterial Nanoparticles for Efficient Hyperthermia in the II and III Near-Infrared Windows

Author

Zhao, Yingqi, Iarossi, Marzia, Maccaferri, Nicolò, Deleye, Lieselot, Melle, Giovanni, Huang, Jian-An, Tantussi, Francesco, Isoniemi, Tommi, and De Angelis, Francesco

Subjects
Physics - Applied Physics, Physics - Biological Physics
Abstract

The use of gold nanoparticles for hyperthermia therapy in near infrared (NIR) spectral regions has catalysed substantial research efforts due to the potential impact in clinical therapy applications. However, the photoscattering effect scaling with the square of the nanoparticle volume leads to a low absorption efficiency, which has hindered the utility of gold nanoparticles in NIR II regions above 1000 nm. Here, we conquer this limit by introducing hyperbolic metamaterial nanoparticles that are made of multi-layered gold/dielectric nanodisks and exhibit >70% absorption efficiency in the NIR II and III regions. Their high light-to-heat conversion is demonstrated by a much larger temperature increase than that of gold nanodisks with the same amount of gold. Efficient in vitro hyperthermia of living cells with negligible cytotoxicity shows the potential of our approach for next-generation bio-medical applications.

Published
2020
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4. Selective intracellular delivery and intracellular recordings combined on MEA biosensors

Author

Cerea, Andrea, Caprettini, Valeria, Bruno, Giulia, Lovato, Laura, Melle, Giovanni, Tantussi, Francesco, Capozza, Rosario, Moia, Fabio, Dipalo, Michele, and De Angelis, Francesco

Subjects
Physics - Applied Physics
Abstract

Biological studies on in vitro cell cultures are of fundamental importance to investigate cells response to external stimuli, such as new drugs for treatment of specific pathologies, or to study communication between electrogenic cells. Although three-dimensional (3D) nanostructures brought tremendous improvements on biosensors used for various biological in vitro studies, including drug delivery and electrical recording, there is still a lack of multifunctional capabilities that could help gaining deeper insights in several bio-related research fields. In this work, the electrical recording of large cell ensembles and the intracellular delivery of few selected cells are combined on the same device by integrating microfluidics channels on the bottom of a multi-electrode array decorated with 3D hollow nanostructures. The novel platform allows to record intracellular-like action potentials from large ensembles of cardiomyocytes derived from human Induced Pluripotent Stem Cells (hiPSC) and from the HL-1 line, while different molecules are selectively delivered into single/few targeted cells. The proposed approach shows high potential for enabling new comprehensive studies that can relate drug effects to network level cell communication processes., Comment: 14 pages, 3 figures

Published
2018

5. Controlled Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes

Author

Huang, Jian-An, Caprettini, Valeria, Zhao, Yingqi, Melle, Giovanni, Maccaferri, Nicolò, Ardini, Matteo, Tantussi, Francesco, Dipalo, Michele, and De Angelis, Francesco

Subjects
Physics - Applied Physics, Physics - Biological Physics, Physics - Optics
Abstract

We present an electrophoretic platform based on 3D hollow nanoelectrodes capable of controlling and quantifying the intracellular delivery of single nanoparticles in single selected cells by surface-enhanced Raman spectroscopy (SERS). The gold-coated hollow nanoelectrode has a sub-femtoliter inner volume that allows the confinement and enhancement of electromagnetic fields upon laser illumination to distinguish the SERS signals of a single nanoparticle flowing through the nanoelectrode. The tight wrapping of cell membranes around the nanoelectrodes enables effective membrane electroporation such that single gold nanorods are delivered into a living cell with a delivery rate subject to the applied bias voltage. The capability of the 3D hollow nanoelectrodes to porate cells and reveal single emitters from the background under live flow is promising for the analysis of both intracellular delivery and sampling., Comment: 8 pages and 5 figures

Published
2018
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9. Hyperbolic metamaterial nanoparticles random array for thermoplasmonics in the II and III near-infrared windows

Author

Zhao, Yingqi, Iarossi, Marzia, Maccaferri, Nicolò, Deleye, Lieselot, Melle, Giovanni, Huang, Jian-An, Iachetta, Giuseppina, D'Amora, Marta, Tantussi, Francesco, Isoniemi, Tommi, De Angelis, Francesco, Zhao, Yingqi, Iarossi, Marzia, Maccaferri, Nicolò, Deleye, Lieselot, Melle, Giovanni, Huang, Jian-An, Iachetta, Giuseppina, D'Amora, Marta, Tantussi, Francesco, Isoniemi, Tommi, and De Angelis, Francesco

Abstract

Plasmonic nanostructures capable of converting light to heat have found wide applications, thus giving rise to the field of thermoplasmonics. Among them, the use of gold-based plasmonic structures in near-infrared (NIR) spectral regions has catalyzed substantial research efforts due to the potential impact in clinical therapy applications. However, the photon scattering effect scaling with the square of the nanoparticle volume leads to high scattering and then low absorption efficiency. This limit has hindered the exploitation of gold nanoparticles, especially in NIR II regions above 1000 nm. Here, we make a step forward for overcoming this limitation by introducing hyperbolic metamaterial nanoparticles that are made of multi-layered gold/dielectric nanodisks and exhibit >70% absorption efficiency in the NIR II and III regions. Their high light-to-heat conversion is demonstrated by a much larger temperature increase than that of gold nanodisks with the same amount of gold. Efficient in vitro hyperthermia of living cells with negligible cytotoxicity shows the potential of our platform for versatile bio-medical applications.

Published
2023
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10. Hyperbolic metamaterial nanoparticles random array for thermoplasmonics in the II and III near-infrared windows

Author

Zhao, Yingqi, primary, Iarossi, Marzia, additional, Maccaferri, Nicolò, additional, Deleye, Lieselot, additional, Melle, Giovanni, additional, Huang, Jian-An, additional, Iachetta, Giuseppina, additional, D'Amora, Marta, additional, Tantussi, Francesco, additional, Isoniemi, Tommi, additional, and De Angelis, Francesco, additional

Published
2023
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16. Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes

Author

Dipalo, Michele, primary, Rastogi, Sahil K., additional, Matino, Laura, additional, Garg, Raghav, additional, Bliley, Jacqueline, additional, Iachetta, Giuseppina, additional, Melle, Giovanni, additional, Shrestha, Ramesh, additional, Shen, Sheng, additional, Santoro, Francesca, additional, Feinberg, Adam W., additional, Barbaglia, Andrea, additional, Cohen-Karni, Tzahi, additional, and De Angelis, Francesco, additional

Published
2021
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17. Cellular Electrophysiological Recording: Mirroring Action Potentials: Label‐Free, Accurate, and Noninvasive Electrophysiological Recordings of Human‐Derived Cardiomyocytes (Adv. Mater. 7/2021)

Author

Barbaglia, Andrea, primary, Dipalo, Michele, additional, Melle, Giovanni, additional, Iachetta, Giuseppina, additional, Deleye, Lieselot, additional, Hubarevich, Aliaksandr, additional, Toma, Andrea, additional, Tantussi, Francesco, additional, and De Angelis, Francesco, additional

Published
2021
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20. Intracellular Recording of Human Cardiac Action Potentials on Market-Available Multielectrode Array Platforms

Author

Melle, Giovanni, Bruno, Giulia, Maccaferri, Nicolò, Iachetta, Giuseppina, Colistra, Nicolò, Barbaglia, Andrea, Dipalo, Michele, De Angelis, Francesco, Melle, Giovanni, Bruno, Giulia, Maccaferri, Nicolò, Iachetta, Giuseppina, Colistra, Nicolò, Barbaglia, Andrea, Dipalo, Michele, and De Angelis, Francesco

Abstract

High quality attenuated intracellular action potentials from large cell networks can be recorded on multi-electrode arrays by means of 3D vertical nanopillars using electrical pulses. However, most of the techniques require complex 3D nanostructures that prevent the straightforward translation into marketable products and the wide adoption in the scientific community. Moreover, 3D nanostructures are often delicate objects that cannot sustain several harsh use/cleaning cycles. On the contrary, laser optoacoustic poration allows the recording of action potentials on planar nanoporous electrodes made of noble metals. However, these constraints of the electrode material and morphology may also hinder the full exploitation of this methodology. Here, we show that optoacoustic poration is also very effective for porating cells on a large family of MEA electrode configurations, including robust electrodes made of nanoporous titanium nitride or disordered fractal-like gold nanostructures. This enables the recording of high quality cardiac action potentials in combination with optoacoustic poration, providing thus attenuated intracellular recordings on various already commercial devices used by a significant part of the research and industrial communities.

Published
2020
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22. Cells adhering to 3D nanoscales: Cell membrane reshaping without stable internalization

Author

Dipalot, Michele, McGuiret, Allister F., Lou, Hsin-Ya, Caprettini, Valeria, Melle, Giovanni, Bruno, Giulia, Lubrano, Claudia, Matino, Laura, De Angelis, Francesco, Cui, Bianxiao, and Santoro, Francesca

Subjects
Electroporation, HEK293 Cells, Surface Properties, Cell Membrane, Cell Adhesion, Humans, Nanotechnology, Myocytes, Cardiac, Cell Shape, Article, Biomechanical Phenomena, Cell Line, Nanostructures
Abstract

The dynamic interface between the cellular membrane and three-dimensional nanostructures determines biological processes and guides the design of novel biomedical devices. Despite recent advancements in the fabrication of artificial bio-interfaces have yielded an enhanced understanding of this interface, there remain open questions on how the cellular membrane reacts and behaves in the presence of sharp objects at the nanoscale. Here, we provide a multifaceted characterization of the cellular membrane’s mechanical stability when closely interacting with high aspect-ratio three-dimensional vertical nanostructures, providing strong evidence that vertical nanostructures spontaneously penetrate the cellular membrane to form a steady intracellular coupling only in rare cases and in specific conditions. The cell membrane is able to conform tightly over the majority of structures with various shapes while maintaining its integrity.

Published
2018

23. Membrane Poration Mechanisms at the Cell–Nanostructure Interface

Author

Dipalo, Michele, primary, Caprettini, Valeria, additional, Bruno, Giulia, additional, Caliendo, Fabio, additional, Garma, Leonardo D., additional, Melle, Giovanni, additional, Dukhinova, Marina, additional, Siciliano, Velia, additional, Santoro, Francesca, additional, and De Angelis, Francesco, additional

Published
2019
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24. On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes

Author

Huang, Jian-An, Caprettini, Valeria, Zhao, Yingqi, Melle, Giovanni, Maccaferri, Nicolò, Deleye, Lieselot, Zambrana-Puyalto, Xavier, Ardini, Matteo, Tantussi, Francesco, Dipalo, Michele, De Angelis, Francesco, Huang, Jian-An, Caprettini, Valeria, Zhao, Yingqi, Melle, Giovanni, Maccaferri, Nicolò, Deleye, Lieselot, Zambrana-Puyalto, Xavier, Ardini, Matteo, Tantussi, Francesco, Dipalo, Michele, and De Angelis, Francesco

Published
2019
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27. Cells Adhering to 3D Vertical Nanostructures: Cell Membrane Reshaping without Stable Internalization

Author

Dipalo, Michele, primary, McGuire, Allister F., additional, Lou, Hsin-Ya, additional, Caprettini, Valeria, additional, Melle, Giovanni, additional, Bruno, Giulia, additional, Lubrano, Claudia, additional, Matino, Laura, additional, Li, Xiao, additional, De Angelis, Francesco, additional, Cui, Bianxiao, additional, and Santoro, Francesca, additional

Published
2018
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30. Longitudinal and Noninvasive Intracellular Recordings of Spontaneous Electrophysiological Activity in Rat Primary Neurons on Planar MEA Electrodes.

Author

Melikov R, Iachetta G, d'Amora M, Melle G, Conti S, Tantussi F, Dipalo M, and De Angelis F

Subjects
Animals, Rats, Cells, Cultured, Electrophysiological Phenomena, Lasers, Neurons physiology, Microelectrodes, Action Potentials physiology
Abstract

Presently, the in vitro recording of intracellular neuronal signals on microelectrode arrays (MEAs) requires complex 3D nanostructures or invasive and approaches such as electroporation. Here, it is shown that laser poration enables intracellular coupling on planar electrodes without damaging neurons or altering their spontaneous electrophysiological activity, allowing the process to be repeated multiple times on the same cells. This capability distinguishes laser-based neuron poration from more invasive methods like electroporation, which typically serve as endpoint measurement for cells. It is demonstrated that planar MEA electrodes, when combined with laser cell optoporation and live cell staining, can record spontaneous intracellular signaling from primary neurons in vitro. This approach allows for the detection of attenuated signals resembling positive monophasic intracellular action potentials. Recordings after laser optoporation also reveal subthreshold signals such as post-synaptic potentials that are essential for assessing neuronal network plasticity and connectivity. Moreover, the noninvasiveness of the process enables repeated intracellular recordings over multiple days from the same cells., (© 2025 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.)

Published
2025
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