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18 results on '"Menéndez Méndez, Aida"'

1. Opposing effects of the purinergic P2X7 receptor on seizures in neurons and microglia in male mice

Author

Alves, Mariana, Gil, Beatriz, Villegas-Salmerón, Javier, Salari, Valentina, Martins-Ferreira, Ricardo, Arribas Blázquez, Marina, Menéndez Méndez, Aida, Da Rosa Gerbatin, Rogerio, Smith, Jonathon, de Diego-Garcia, Laura, Conte, Giorgia, Sierra-Marquez, Juan, Merino Serrais, Paula, Mitra, Meghma, Fernandez Martin, Ana, Wang, Yitao, Kesavan, Jaideep, Melia, Ciara, Parras, Alberto, Beamer, Edward, Zimmer, Béla, Heiland, Mona, Cavanagh, Brenton, Parcianello Cipolat, Rafael, Morgan, James, Teng, Xinchen, Prehn, Jochen H.M., Fabene, Paolo F., Bertini, Giuseppe, Artalejo, Antonio R., Ballestar, Esteban, Nicke, Annette, Olivos-Oré, Luis A., Connolly, Niamh M.C., Henshall, David C., and Engel, Tobias

Published
2024
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4. The P2X7 receptor contributes to seizures and inflammation‐driven long‐lasting brain hyperexcitability following hypoxia in neonatal mice

Author

Smith, Jonathon, primary, Menéndez Méndez, Aida, additional, Alves, Mariana, additional, Parras, Alberto, additional, Conte, Giorgia, additional, Bhattacharya, Anindya, additional, Ceusters, Marc, additional, Nicke, Annette, additional, Henshall, David C., additional, Jimenez‐Mateos, Eva M., additional, and Engel, Tobias, additional

Published
2023
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5. Increased uptake of the P2X7 receptor radiotracer18 F‐JNJ‐64413739 in the brain and peripheral organs according to the severity of status epilepticus in male mice

Author

Morgan, James, primary, Moreno, Oscar, additional, Alves, Mariana, additional, Baz, Zuriñe, additional, Menéndez Méndez, Aida, additional, Leister, Hanna, additional, Melia, Ciara, additional, Smith, Jonathon, additional, Visekruna, Alexander, additional, Nicke, Annette, additional, Bhattacharya, Anindya, additional, Ceusters, Marc, additional, Henshall, David C., additional, Gómez‐Vallejo, Vanessa, additional, Llop, Jordi, additional, and Engel, Tobias, additional

Published
2022
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7. Increased expression of the ATP‐gated P2X7 receptor reduces responsiveness to anti‐convulsants during status epilepticus in mice

Author

Beamer, Edward, primary, Morgan, James, additional, Alves, Mariana, additional, Menéndez Méndez, Aida, additional, Morris, Gareth, additional, Zimmer, Béla, additional, Conte, Giorgia, additional, de Diego‐Garcia, Laura, additional, Alarcón‐Vila, Cristina, additional, Yiu Ng, Nico Ka, additional, Madden, Stephen, additional, Calzaferri, Francesco, additional, de los Ríos, Cristóbal, additional, García, Antonio G., additional, Hamacher, Michael, additional, Dinkel, Klaus, additional, Pelegrín, Pablo, additional, Henshall, David C., additional, Nicke, Annette, additional, and Engel, Tobias, additional

Published
2022
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8. Increased uptake of the P2X7 receptor radiotracer 18F‐JNJ‐64413739 in the brain and peripheral organs according to the severity of status epilepticus in male mice.

Author

Morgan, James, Moreno, Oscar, Alves, Mariana, Baz, Zuriñe, Menéndez Méndez, Aida, Leister, Hanna, Melia, Ciara, Smith, Jonathon, Visekruna, Alexander, Nicke, Annette, Bhattacharya, Anindya, Ceusters, Marc, Henshall, David C., Gómez‐Vallejo, Vanessa, Llop, Jordi, and Engel, Tobias

Subjects
STATUS epilepticus, RADIOACTIVE tracers, TEMPORAL lobe epilepsy, POSITRON emission tomography, MAGNETIC resonance imaging
Abstract

Objective: The P2X7 receptor (P2X7R) is an important contributor to neuroinflammation, responding to extracellularly released adenosine triphosphate. Expression of the P2X7R is increased in the brain in experimental and human epilepsy, and genetic or pharmacologic targeting of the receptor can reduce seizure frequency and severity in preclinical models. Experimentally induced seizures also increase levels of the P2X7R in blood. Here, we tested 18F‐JNJ‐64413739, a positron emission tomography (PET) P2X7R antagonist, as a potential noninvasive biomarker of seizure‐damage and epileptogenesis. Methods: Status epilepticus was induced via an intra‐amygdala microinjection of kainic acid. Static PET studies (30 min duration, initiated 30 min after tracer administration) were conducted 48 h after status epilepticus via an intravenous injection of 18F‐JNJ‐64413739. PET images were coregistered with a brain magnetic resonance imaging atlas, tracer uptake was determined in the different brain regions and peripheral organs, and values were correlated to seizure severity during status epilepticus. 18F‐JNJ‐64413739 was also applied to ex vivo human brain slices obtained following surgical resection for intractable temporal lobe epilepsy. Results: P2X7R radiotracer uptake correlated strongly with seizure severity during status epilepticus in brain structures including the cerebellum and ipsi‐ and contralateral cortex, hippocampus, striatum, and thalamus. In addition, a correlation between radiotracer uptake and seizure severity was also evident in peripheral organs such as the heart and the liver. Finally, P2X7R radiotracer uptake was found elevated in brain sections from patients with temporal lobe epilepsy when compared to control. Significance: Taken together, our data suggest that P2X7R‐based PET imaging may help to identify seizure‐induced neuropathology and temporal lobe epilepsy patients with increased P2X7R levels possibly benefitting from P2X7R‐based treatments. [ABSTRACT FROM AUTHOR]

Published
2023
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11. Novel Point-of-Care Diagnostic Method for Neonatal Encephalopathy Using Purine Nucleosides

Author

Beamer, Edward, primary, O’Dea, Mary Isabel, additional, Garvey, Aisling A., additional, Smith, Jonathon, additional, Menéndez-Méndez, Aida, additional, Kelly, Lynne, additional, Pavel, Andreea, additional, Quinlan, Sean, additional, Alves, Mariana, additional, Jimenez-Mateos, Eva M., additional, Tian, Faming, additional, Dempsey, Eugene, additional, Dale, Nicholas, additional, Murray, Deirdre M., additional, Boylan, Geraldine B., additional, Molloy, Eleanor J., additional, and Engel, Tobias, additional

Published
2021
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12. Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells

Author

Menéndez-Méndez, Aida, Paniagua-Herranz, Lucía, Olivos Ore, Luis Alcides, Gómez Villafuertes, María Rosa, Rodríguez Artalejo, Antonio, Pérez Sen, Raquel, García Delicado, Esmerilda, Ortega De La O, Felipe, Menéndez-Méndez, Aida, Paniagua-Herranz, Lucía, Olivos Ore, Luis Alcides, Gómez Villafuertes, María Rosa, Rodríguez Artalejo, Antonio, Pérez Sen, Raquel, García Delicado, Esmerilda, and Ortega De La O, Felipe

Abstract

Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings. For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b)., Government of Madrid (PR65/19-22453), Spanish Ministerio de Ciencia, Innovacioín y Universidades MCIU, (PID2019-109155RB-I00, BFU2015-70067REDC)., Sección Deptal. de Farmacología y Toxicología (Veterinaria), Fac. de Veterinaria, TRUE, pub

Published
2021

13. Circulating P2X7 receptor signaling components as diagnostic biomarkers for temporal lobe epilepsy

Author

Conte, Giorgia, Menéndez-Méndez, Aida, Bauer, Sebastian, El-Naggar, Hany, Alves, Mariana, Nicke, Annette, Delanty, Norman, Rosenow, Felix, Henshall, David C., Engel, Tobias, Conte, Giorgia, Menéndez-Méndez, Aida, Bauer, Sebastian, El-Naggar, Hany, Alves, Mariana, Nicke, Annette, Delanty, Norman, Rosenow, Felix, Henshall, David C., and Engel, Tobias

Abstract

Circulating molecules have potential as biomarkers to support the diagnosis of epilepsy and to assist with differential diagnosis, for example, in conditions resembling epilepsy, such as in psychogenic non-epileptic seizures (PNES). The P2X7 receptor (P2X7R) is an important regulator of inflammation and mounting evidence supports its activation in the brain during epilepsy. Whether the P2X7R or P2X7R-dependent signaling molecules can be used as biomarkers of epilepsy has not been reported. P2X7R levels were analyzed by quantitative ELISA using plasma samples from controls and patients with temporal lobe epilepsy (TLE) or PNES. Moreover, blood cell P2X7R expression and P2X7R-dependent cytokine signature was measured following status epilepticus in P2X7R-EGFP reporter, wildtype, and P2X7R-knockout mice. P2X7R plasma levels were higher in TLE patients when compared with controls and patients with PNES. Plasma levels of the broad inflammatory marker protein C-Reactive protein (CRP) were similar between the three groups. Using P2X7R-EGFP reporter mice, we identified monocytes as the main blood cell type expressing P2X7R after experimentally evoked seizures. Finally, cytokine array analysis in P2X7R-deficient mice identified KC/GRO as a potential P2X7R-dependent plasma biomarker following status epilepticus and during epilepsy. Our data suggest that P2X7R signaling components may be a promising subclass of circulating biomarkers to support the diagnosis of epilepsy.

Published
2021

17. Specific Temporal Distribution and Subcellular Localization of a Functional Vesicular Nucleotide Transporter (VNUT) in Cerebellar Granule Neurons

Author

Menéndez-Méndez, Aida, Díaz-Hernández, Juan I, Ortega, Felipe, Gualix, Javier, Gómez-Villafuertes, Rosa, and Miras-Portugal, María T

Subjects
cerebellum, VGLUT1, ATP exocytosis, VNUT, GCPs, granule cells
Abstract

Adenosine triphosphate (ATP) is an important extracellular neurotransmitter that participates in several critical processes like cell differentiation, neuroprotection or axon guidance. Prior to its exocytosis, ATP must be stored in secretory vesicles, a process that is mediated by the Vesicular Nucleotide Transporter (VNUT). This transporter has been identified as the product of the SLC17A9 gene and it is prominently expressed in discrete brain areas, including the cerebellum. The main population of cerebellar neurons, the glutamatergic granule neurons, depends on purinergic signaling to trigger neuroprotective responses. However, while nucleotide receptors like P2X7 and P2Y13 are known to be involved in neuroprotection, the mechanisms that regulate ATP release in relation to such events are less clearly understood. In this work, we demonstrate that cerebellar granule cells express a functional VNUT that is involved in the regulation of ATP exocytosis. Numerous vesicles loaded with this nucleotide can be detected in these granule cells and are staining by the fluorescent ATP-marker, quinacrine. High potassium stimulation reduces quinacrine fluorescence in granule cells, indicating they release ATP via calcium dependent exocytosis. Specific subcellular markers were used to assess the localization of VNUT in granule cells, and the transporter was detected in both the axonal and somatodendritic compartments, most predominantly in the latter. However, co-localization with the specific lysosomal marker LAMP-1 indicated that VNUT can also be found in non-synaptic vesicles, such as lysosomes. Interestingly, the weak co-localization between VNUT and VGLUT1 suggests that the ATP and glutamate vesicle pools are segregated, as also observed in the cerebellar cortex. During post-natal cerebellar development, VNUT is found in granule cell precursors, co-localizing with markers of immature cells like doublecortin, suggesting that this transporter may be implicated in the initial stages of granule cell development.

Published
2017

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