21 results on '"Meng, Ronggui"'
Search Results
2. Predictive value of modified MRI-based split scar sign (mrSSS) score for pathological complete response after neoadjuvant chemoradiotherapy for patients with rectal cancer
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Yuan, Yuan, Zheng, Kuo, Zhou, Lu, Chen, Fangying, Zhang, Shaoting, Lu, Haidi, Lu, Jianping, Shao, Chengwei, Meng, Ronggui, Zhang, Wei, Gao, Xianhua, and Shen, Fu
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- 2023
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3. A novel 9-gene signature for the prediction of postoperative recurrence in stage II/III colorectal cancer
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Xin, Cheng, primary, Lai, Yi, additional, Ji, Liqiang, additional, Wang, Ye, additional, Li, Shihao, additional, Hao, Liqiang, additional, Zhang, Wei, additional, Meng, Ronggui, additional, Xu, Jun, additional, Hong, Yonggang, additional, and Lou, Zheng, additional
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- 2023
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4. Multicenter analysis of risk factors for anastomotic leakage after middle and low rectal cancer resection without diverting stoma: a retrospective study of 319 consecutive patients
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Zhang, Wei, Lou, Zheng, Liu, Qizhi, Meng, Ronggui, Gong, Haifeng, Hao, Liqiang, Liu, Peng, Sun, Ge, Ma, Jun, and Zhang, Wei
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- 2017
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5. Trends of sphincter-preserving surgeries for low lying rectal cancer: A 20-year experience in China
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Zheng, Kuo, primary, Hu, Qingqing, additional, Yu, Guanyu, additional, Zhou, Leqi, additional, Yao, Yuting, additional, Zhou, Yuan, additional, Wang, Hao, additional, Hao, Liqiang, additional, Yu, Enda, additional, Lou, Zheng, additional, Zhang, Yongjing, additional, Qiu, Hong, additional, Meng, Ronggui, additional, and Zhang, Wei, additional
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- 2022
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6. Comparison of Functional and Oncological Outcome of Conformal Sphincter Preservation Operation, Low Anterior Resection and Abdominoperineal Resection in Very Low Rectal Cancer; A Retrospective Comparative Cohort Study with Propensity Score Matching
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Sun, Ge, primary, Lou, Zheng, additional, Zheng, Kuo, additional, Chen, Yuntao, additional, Zhang, Hang, additional, Wen, Rongbo, additional, Gao, Xianhua, additional, Meng, Ronggui, additional, Gong, Haifeng, additional, Bai, Chenguang, additional, Furnée, Edgar J.B., additional, and Zhang, Wei, additional
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- 2022
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7. Trend of sphincter-preserving surgeries for resectable low rectal cancer: A 20-year experience in China.
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HU, Qingqing, primary, Zheng, Kuo, additional, Yao, Yuting, additional, Zhou, Yuan, additional, Meng, Ronggui, additional, Wang, Hao, additional, Hao, Liqiang, additional, Zhang, Yongjing, additional, Qiu, Hong, additional, and Zhang, Wei, additional
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- 2022
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8. Influence of neoadjuvant chemoradiotherapy on the anal sphincter: ultrastructural damage may be critical
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Zhu, Xiaoming, Lou, Zheng, Gong, Haifeng, Meng, Ronggui, Hao, Liqiang, and Zhang, Wei
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- 2016
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9. The Classification and Surgical Treatments in Adult Hirschsprung’s Disease: A Retrospective Study
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Ma, Shengzhe, primary, Yu, Yue, additional, Pan, Anfu, additional, Gong, Haifeng, additional, Lou, Zheng, additional, Liu, Lianjie, additional, Hao, Liqiang, additional, Meng, Ronggui, additional, Sui, Jinke, additional, and Zhang, Wei, additional
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- 2022
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10. Lower rate of colonoscopic perforation: 110,785 patients of colonoscopy performed by colorectal surgeons in a large teaching hospital in China
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Shi, Xiaohui, Shan, Yongqi, Yu, Enda, Fu, Chuangang, Meng, Ronggui, Zhang, Wei, Wang, Hantao, Liu, Lianjie, Hao, Liqiang, Wang, Hao, Lin, Miao, Xu, Honglian, Xu, Xiaodong, Gong, Haifeng, Lou, Zheng, He, Haiyan, Xing, Junjie, Gao, Xianhua, and Cai, Beili
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- 2014
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11. 适形切除保肛术与经括约肌间切除术治疗低位直肠癌的临床疗效
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Sun, Ge, Zang, Yiwen, Ding, Haibo, Chen, Yuntao, Gong, Haifeng, Lou, Zheng, Hao, Liqiang, Meng, Ronggui, Chen, Zongyou, Xiang, Jianbin, and Zhang, Wei
- Subjects
Oncological safety ,Rectal neoplasms ,Intersphinc-teric resection ,Anal function ,Conformal sphincter preservation operation - Abstract
Objective: To investigate clinical efficacy of conformal sphincter preservation operation (CSPO) versus intersphincteric resection (ISR) in the treatment of low rectal cancer. Methods: The retrospective cohort study was conducted. The clinicopathological data of 183 patients with low rectal cancer who were admitted to two medical centers (117 in the Changhai Hospital of Naval Medical University and 66 in the Huashan Hospital of Fudan University) from August 2011 to April 2020 were collected. There were 110 males and 73 females, aged (57±11)years. Of 183 patients, 117 cases undergoing CSPO were allocated into CSPO group, and 66 cases undergoing ISR were allocated into ISR group, respectively. Observation indicators: (1) surgical situations of patients with low rectal cancer in the two groups; (2) postoperative complications of patients with low rectal cancer in the two groups; (3) follow-up; (4) influencing factors for prognosis of patients with low rectal cancer; (5) influencing factors for satisfaction with the anal function of patients with low rectal cancer. Follow-up was conducted using outpatient examination, questionnaire and telephone interview to determine local recurrence, distal metastasis, survival, stomal closure, satisfaction with the anal function of patients. Measurement data with normal distribution were represented as Mean±SD, and comparison between groups was analyzed using the t test. Measurement data with skewed distribution were represented as M (range). Count data were described as absolute numbers or percentages, and comparison between groups was analyzed using the chi-square test. Comparison of ordinal data was analyzed using the rank sum test.The Kaplan-Meier method was used to draw survival curves, and life table method was used to calculate survival rates. Log-rank test was used for survival analysis. Univariate analysis was performed using the linear regression. Variables with P0.05). Patients with complications in the two groups were improved after symptomatic and supportive treatment. There was no perioperative death in the postoperative 30 days of the two groups. (3) Follow-up: 183 patients received follow-up. Patients of the CSPO group and ISR group were followed up for (41±27)months and (37±19)months, respectively, showing no significant difference between the two groups (t=-1.104, P>0.05). There were 2 cases with local recurrence and 9 cases with distal metastasis of the CSPO group, respectively, versus 3 cases and 4 cases of the ISR group, showing no significant difference between the two groups (χ2=1.277, 0.170, P>0.05). The 3-year disease-free survival rate and 3-year total survival rate were 84.0% and 99.0% for the CSPO group, versus 88.6% and 92.8% for the ISR group, showing no significant difference between the two groups (χ2=0.218, 0.002, P>0.05). The stomal closure rate was 92.16%(94/102) and 96.97%(64/66) for 102 patients of CSPO group and 66 patients of ISR group up to postoperative 12 months,respectively, showing no significant difference between the two groups (χ2=1.658, P>0.05). Of the 8 cases without stomal closure in the CSPO group, 2 cases refused due to advanced age, 4 cases subjectively refused, and 2 cases were irreducible due to scar caused by radiotherapy. Two cases in the ISR group had no stomal closure including 1 case of postoperative liver metastasis and 1 case of subjective refusal. There were 92 and 61 patients followed up to 12 months after stomal closure, of which 75 cases and 38 cases completed questionnaires of satisfaction with the anal function. The satisfaction score with the anal function was 6.8±2.8 and 5.4±3.0 for CSPO group and ISR group, respectively, showing a significant difference between the two groups (t=-2.542, P5, showing no significant difference between the two groups (χ2=3.165, P>0.05). (4) Influencing factors for prognosis of patients with low rectal cancer: results of COX stepwise regression analysis showed that gender and pT staging were independent influencing factors for disease-free survival rate of patients with low rectal cancer (hazard ratio=2.883, 1.963, 95% confidence interval as 1.090 to 7.622, 1.129 to 3.413, P
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- 2021
12. Pull-through and conformal resection for very low rectal cancer: a more satisfactory technique for anal function after sphincter preserving operation
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Lou, Zheng, primary, Gong, Haifeng, additional, He, Jian, additional, Zhu, Xiaoming, additional, Meng, Ronggui, additional, and Zhang, Wei, additional
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- 2016
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13. Colonoscopy is the first choice for early postoperative rectal anastomotic bleeding
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Lou, Zheng, primary, Zhang, Wei, additional, Yu, Enda, additional, Meng, Ronggui, additional, and Fu, Chuangang, additional
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- 2014
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14. Clinicopathological features and prognosis of synchronous and metachronous colorectal cancer: a retrospective cohort study.
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Fan H, Wen R, Zhou L, Gao X, Lou Z, Hao L, Meng R, Gong H, Yu G, and Zhang W
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- Humans, Retrospective Studies, China epidemiology, Prognosis, Neoplasms, Multiple Primary surgery, Neoplasms, Second Primary surgery, Colorectal Neoplasms pathology
- Abstract
Background: To investigate the clinicopathological features and prognosis of synchronous and metachronous multiple primary colorectal cancer., Materials and Methods: Patients who underwent operation for synchronous and metachronous colorectal cancer at the colorectal surgery department of Shanghai Changhai Hospital between January 2000 and December 2021 were included. Perioperative indicators were comprehensively compared and included in the survival analyses., Results: In total, 563 patients with synchronous ( n =372) and metachronous ( n =191) colorectal cancer were included. Patients with synchronous colorectal cancer were more likely to have a long onset time, positive carcinoembryonic antigen, advanced TNM stage, large tumor, perineural invasion, p53 high expression, and mismatch repair proficient. Compared with metachronous colorectal cancer, patients with synchronous colorectal cancer showed worse 5-year overall survival (68.6±3.0% vs 81.9±3.5%, P =0.018) and 5-year disease-free survival (61.2±3.1% vs 71.0±3.9%, P =0.022). In the subgroup analysis, segmental resection was an independent risk factor for the long-term outcomes of bilateral synchronous colorectal cancer., Conclusions: Clinicopathological and molecular features were different between synchronous and metachronous colorectal cancer. Patients with synchronous colorectal cancer showed a worse prognosis than those with metachronous colorectal cancer. Bilateral synchronous colorectal cancer requires extended resection to achieve improved long-term outcomes., (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)
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- 2023
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15. [A single center retrospective study on surgical efficacy of T3NxM0 middle-low rectal cancer without neoadjuvant therapy].
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Liu P, Lou Z, Mei Z, Gao X, Hao L, Liu L, Gong H, Meng R, Yu E, Wang H, Wang H, and Zhang W
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- Humans, Lymph Node Excision, Lymph Nodes pathology, Lymph Nodes surgery, Lymphatic Metastasis, Mesocolon surgery, Neoadjuvant Therapy, Neoplasm Staging, Prognosis, Rectal Neoplasms pathology, Retrospective Studies, Proctectomy methods, Rectal Neoplasms surgery
- Abstract
Objective: To investigate the surgical efficacy and prognostic factors of T3NxM0 middle-low rectal cancer without neoadjuvant therapy., Methods: Clinical data of patients with middle-low rectal cancer undergoing TME surgery with T3NxM0 confirmed by postoperative pathology at Colorectal Surgery Department of Changhai Hospital from January 2008 to December 2010 were analyzed retrospectively., Inclusion Criteria: (1)no preoperative neoadjuvant chemoradiotherapy (nCRT); (2) complete preoperative evaluation, including medical history, preoperative colonoscopy or digital examination, blood tumor marker examination, and imaging examination; (3) distance between tumor lower margin and anal verge was ≤ 10 cm; (4) negative circumferential resection margin (CRM-). Finally, a total of 331 patients were included in this study. According to the number of metastatic lymph node confirmed by postoperative pathology, the patients were divided into N0 group without regional lymph node metastasis (190 cases) and N+ group with regional lymph node metastasis (141 cases). The perioperative conditions, local recurrence, distant metastasis and prognostic factors were analyzed., Results: Compared to N0 group in the perioperative data, N+ group had higher ratio of tumor deposit [29.8%(42/141) vs. 0, χ²=64.821, P<0.001] and vascular invasion [7.1%(10/141) vs. 0.5%(1/190),χ²=10.860, P<0.001]. There were no significant differences in tumor diameter, number of lymph nodes detected, positive nerve invasion, degree of tumor differentiation, morbidity of postoperative complication and postoperative adjuvant chemotherapy rate between the two groups (all P>0.05). The median follow-up period was 73.4 months. The merged 5-year local recurrence rate was 2.7%(9/331), 5-year distant metastasis rate was 23.3% (77/331), 5-year disease-free survival (DFS) rate was 73.4%, and 5-year overall survival (OS) rate was 77.2%. Multivariate analysis showed that lymph node metastasis (HR=3.120, 95%CI: 1.918 to 5.075, P<0.001), nerve invasion (HR=0.345, 95%CI: 0.156 to 0.760, P=0.008) and vascular invasion (HR=0.428, 95%CI: 0.189 to 0.972, P=0.043) were independent risk factors for DFS in patients with T3NxM0 rectal cancer after operation. Preoperative carcinoembryonic antigen level (HR=1.858, 95%CI:1.121 to 3.079, P=0.016), lymph node metastasis (HR=3.320, 95%CI: 1.985 to 5.553, P<0.001) and nerve invasion (HR=0.339, 95%CI: 0.156 to 0.738, P=0.006) were independent risk factors for OS in patients with T3NxM0 rectal cancer after operation., Conclusions: Optimal local control rate of middle-low rectal cancer patients with T3NxM0 and CRM- can be achieved by standard TME surgery alone. For patients with preoperative elevated blood carcinoembryonic antigen level, regional lymph node metastasis, or neurovascular invasion confirmed by pathology after surgery, adjuvant chemoradiotherapy should be actively applied after surgery to improve prognosis.
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- 2019
16. [Study on the relationship between tumor regression grade and lymph node regression grade].
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Zhao Q, Fu C, Yu E, Zhang W, Meng R, Wang H, Hao L, and Wang H
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- Adult, Aged, Female, Humans, Male, Middle Aged, Neoadjuvant Therapy, Neoplasm Grading, Rectal Neoplasms drug therapy, Rectal Neoplasms radiotherapy, Rectal Neoplasms surgery, Retrospective Studies, Chemoradiotherapy, Adjuvant, Lymph Nodes pathology, Rectal Neoplasms pathology
- Abstract
Objective: To investigate the relationship between tumor regression grade (TRG) and lymph node regression grade (LRG) after neoadjuvant chemoradiotherapy (CRT) for rectal cancer and its clinical implication., Methods: Clinicopathological data of 176 rectal cancer patients undergoing radical excision after neoadjuvant CRT from January 2005 to December 2013 in our department were retrospectively analyzed., Inclusion Criteria: (1) Radiology indicated locally advanced low rectal cancer and patients had strong desire to preserve the sphincter before neoadjuvant CRT; (2) there was no definite metastatic lesion before neoadjuvant CRT; (3) patients received whole course of neoadjuvant CRT (regular radiotherapy plus synchronous fluorouracil-like drugs chemotherapy); (4) patients underwent radical operation after neoadjuvant CRT. Patients with short-course CRT and emergency surgery were excluded. TRG and LRG of postoperative specimens (including tumor and lymph nodes) were carried out based on the percentage of the fibrosis and the cancer residue. No cancer residue was defined as TRG1 and LRG1; rare cancer cell residue as TRG2 and LRG2; fibrosis growth over residual cancer as TRG3 and LRG3; residual cancer growth over fibrosis as TRG4 and LRG4; absence of regressive changes as TRG5 and LRG5; and normal lymph nodes as LRG0. Spearman correlation test was used to assess the correlation between TRG and LRG., Results: Of 176 patients, 111 were men and 65 were women. The mean age was (53.9±13.0) years. The number of patients with stage I(, II(, and III( before operation was 10, 49 and 62 while other 55 patients were unknown. Transabdominal low anterior resection (LAR) was performed in 118 cases and abdominal-perineal resection(APR) in 47 cases following the principle of total mesorectal excision (TME). Postoperative pathology of specimens revealed that the number of patients from TRG1 to TRG5 was 19 (10.8%), 25 (14.2%), 66 (37.5%), 47 (26.7%), 19 (10.8%), and from LRG0 to LRG5 was 35 (19.9%), 68 (38.6%), 10 (5.7%), 14 (8.0%), 15(8.5%), 34 (19.3%), respectively. TRG was correlated to LRG (P=0.005) while the Spearman correlation coefficient was only 0.24. The analysis of subgroup without LRG1 also showed that TRG was correlated to LRG(P=0.0005) and the Spearman correlation coefficient was 0.40., Conclusions: TRG can not represent LRG. Therefore, both TRG and LRG should be assessed when evaluating the response of rectal cancer to neoadjuvant CRT.
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- 2017
17. [Preliminary investigation of intramural lateral spread distance in pull-through conformal resection of low rectal cancer].
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Zhu X, Lou Z, Bai C, Gong H, Ma J, Meng R, Hao L, and Zhang W
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- Humans, Margins of Excision, Multivariate Analysis, Neoplasm Staging adverse effects, Risk Factors, Digestive System Surgical Procedures methods, Neoplasm Invasiveness pathology, Rectal Neoplasms pathology, Rectal Neoplasms surgery, Rectum surgery
- Abstract
Objective: To investigate the intramural lateral spread distance in low rectal cancer in order to provide basis for safety lateral resection margin of pull-through conformal resection (PTCR)., Methods: The patients with low rectal cancer who received low anterior resection or abdominal-perineal resection in Changhai Hospital from December 2015 to March 2016 were enrolled and Surgical specimens were collected. After the specimens were fixed in 10% formaldehyde for 24 hours, a piece of tissue that was 1.5 cm in length and 0.5 cm in width from the edge of tumor was cut. The tissue was obtained in the direction of 3, 5, 7 and 9 o'clock clockwise. The distance of intramural lateral spread was measured in the specimens and the risk factors were analyzed., Results: A total of 83 specimens were collected and the overall proportion of intramural lateral spread was 71.1%(59/83). The rate of lateral spread from 3 to 9 o'clock was 34.9%(29/83), 26.5%(22/83), 32.5%(27/83) and 37.3%(31/83) respectively, and the difference was not statistically significant(χ
2 =2.444 9, P=0.485 3). The median distance of lateral spread in each direction was all 0 mm and the quartile range was 1 mm, 0.5 mm, 0.55 mm and 1 mm respectively. The 5th percentile (P5) of each direction was all 0 mm and the 95th percentile(P95) of each direction was 2.5 mm, 1.6 mm, 2.6 mm, 2.5 mm, respectively and the difference was not statistically significant either(χ2 =5.331 0, P=0.148 9). The rate of lateral spread of T1, T2, T3 and T4 was 0/4, 58.3%(14/24), 83.0%(44/53) and 1/2 respectively, and there was significant difference(P=0.005 0). The multivariate analysis indicated that T stage (P=0.002 2, OR=3.741, 95% CI: 1.606-8.716) was the risk factor of intramural lateral spread., Conclusions: The intramural lateral spread does exist in low rectal cancer and T stage is the risk factor of lateral spread. The lateral resection margin should be 5 mm from the tumor edge at least when PTCR is performed.- Published
- 2016
18. [Assessment of the risk factors relating to lymph node metastasis in rectal cancer after neoadjuvant chemoradiotherapy and the clinical significance].
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Zhao Q, Shi X, Fu C, Yu E, Zhang W, Meng R, Wang H, Hao L, and Wang H
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- Age Factors, Carcinoembryonic Antigen blood, Chemoradiotherapy, Female, Humans, Male, Neoplasm Grading, Neoplasm Invasiveness, Rectal Neoplasms therapy, Remission Induction, Retrospective Studies, Risk Factors, Treatment Outcome, Lymphatic Metastasis diagnosis, Neoadjuvant Therapy, Rectal Neoplasms complications, Rectal Neoplasms epidemiology
- Abstract
Objective: To identify the risk factors associated with lymph node metastasis in rectal cancer after neoadjuvant chemoradiotherapy (CRT)., Methods: From January 2005 to December 2013, the clinical data of 178 patients with advanced rectal cancer undergoing radical excision after neoadjuvant CRT in our department were reviewed retrospectively. A total of 11 clinicopathologic factors relating to lymph node metastasis were studied using univariate and multivariate Logistic regression analyses., Results: There were 74(41.6%) cases with lymph node metastasis, while 104 cases without lymph node metastasis. Univariate analysis showed that age(P=0.000 2), post-CRT CEA level(P=0.011 2), ypT stage(P=0.000 0), pathologic type(P=0.004 0), and tumor regression grade(TRG)(P=0.033 8) were significantly associated with lymph node metastasis. Multivariate analysis showed that age(OR=2.385, 95% CI:1.372 ~ 4.147, P=0.002 1), post-CRT CEA level(OR=2.310, 95% CI:1.005 ~ 5.307, P=0.048 6) and ypT stage(OR=2.592, 95% CI:1.236 ~ 5.432, P=0.011 7) were independent risk factors. However, 15.8% of the patients who achieved TRG1 had lymph node metastasis and TRG failed to independently correlate with lymph node metastasis in rectal cancer after neoadjuvant CRT., Conclusions: There was a higher ratio of lymph node metastasis in rectal cancer patients who were young, CEA≥5 μg/L or deep invasion after neoadjuvant CRT. Therefore, neoadjuvant CRT should be carefully considered in these patients.
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- 2016
19. [Analysis of risk factors associate with lymph node metastasis of T1 and T2 rectal cancer].
- Author
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He J, Lou Z, Zhang W, Meng R, Fu C, Yu E, Wang H, Hao L, and Wang H
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- Humans, Lymph Nodes, Neoplasm Invasiveness, Prognosis, Rectal Neoplasms pathology, Retrospective Studies, Risk Factors, Lymphatic Metastasis, Neoplasm Staging, Rectal Neoplasms surgery
- Abstract
Objective: To investigate the risk factors associated with lymph node metastasis of T1 and T2 rectal cancer., Methods: Clinicopathological data of 576 patients with stage T1 to T2 rectal cancer without serosal invasion confirmed by pathology undergoing curative resection in Changhai Hospital from January 1999 to December 2013 were analyzed retrospectively. The relationship of clinicopathological factors of overall patients and stage T1 patients with lymph node metastasis was analyzed by univariate or multivariate analysis., Results: The lymph node metastasis rate of stage T2 rectal cancer was significantly higher than that of stage T1[22.9% (108/463) vs. 9.7%(11/113), P=0.002], and the difference of stage T2a and T2b was not significant[22.0%(38/173) vs. 23.4% (68/290), P=0.733]. Multivariate analysis showed that poor differentiation(HR=1.54, 95% CI:1.12 to 2.13), abnormal carbohydrate antigen (CA) 199 level (HR=2.05, 95% CI:1.16 to 3.62), ulcerative mass (HR=1.58, 95% CI:1.05 to 2.39) and invasion of muscle (of inner ring muscle HR=3.55, 95% CI:1.79 to 7.02; of outer longitudinal muscle, HR=2.35, 95% CI:1.21 to 4.60) were independent risk factors of lymph node metastasis in patients with stage T1-T2 rectal cancer(all P<0.05). Meanwhile poor differentiation(HR=4.43, 95% CI:1.51 to 13.03), abnormal carcinoembryonic antigen(CEA) level (HR=4.66, 95% CI:1.18 to 20.11) and ulcerative mass (HR=6.23, 95% CI:1.51 to 25.66) were risk factors of lymph node metastasis in patients with stage T1 rectal cancer., Conclusion: Poor differentiation, preoperative high CA199, ulcerated tumor, invasion of inner ring muscle or outer longitudinal muscle are risk factors of lymph node metastasis in patients with stage T1-T2 rectal cancer, while the invasion depth of muscularis propria is not risk factor. Besides, poor differentiation, abnormal CEA level, ulcerated tumor are risk factors of lymph node metastasis in stage T1 rectal cancer patients, which can be used as reference for local excision in patients with stage T1 rectal cancer.
- Published
- 2015
20. [RhoA gene expression in colorectal carcinoma].
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Wang H, Chen Y, Cao D, Zhang Y, Meng R, and Lu J
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- Adult, Aged, Aged, 80 and over, Colorectal Neoplasms pathology, Female, Humans, Male, Middle Aged, Neoplasm Metastasis genetics, RNA, Messenger biosynthesis, rhoA GTP-Binding Protein biosynthesis, Colorectal Neoplasms genetics, Gene Expression, rhoA GTP-Binding Protein genetics
- Abstract
Objective: To investigate the expression of RhoA mRNA in colorectal neoplasms., Methods: Total RNA was extracted from 42 surgically resected specimens of colorectal caricinoma and normal mucosa close to the lesions. Afier the optimal reverse transcription polymerase chain reaction (RT-PCR) condition was found, the mRNA expression levels of the RhoA gene in the lesions and the normal mucosa were examined semi-quantitatively and the relation between these expression levels and clinical pathological markers was compared., Results: The expression of RhoA gene mRNA in lesion of colorectal carcinoma was significantly higher than that in contiguous normal mucosa (1.8 +/- 0.6 vs 0.9 +/- 0.4, P < 0.01). The ratio of RhoA expression in neoplasm to that in contiguous mucosa was 2.02 +/- 0.82. Based on this ratio the 42 patients were divided into two groups: overexpression group (19 cases) and high-expression group (23 cases). In the overexpression group the lesions of 14 cases were in Dukes C and Dukes D stages, and lymph node metastasis was found in 13 cases. In the high-expression group, only 9 out of the 23 cases were in advanced stage and 8 cases showed lymph-node metastasis. Chi-squared test analysis demonstrated a significant difference in both aspects between these two groups (P < 0.05). The difference in number of metastatic lymph nodes between the two groups was statistically significant (P < 0.01). Mutation was not found in the RhoA gene sequence examined., Conclusion: RhoA gene may play an important role in colorectal carcinogenesis and correlate with invasion and metastasis of colorectal carcinoma.
- Published
- 2002
21. [Gene expression significance of beta-catenin, p53 and PCNA in PJS polyposis].
- Author
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Zhang W, Meng R, Fu C, and Yu D
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- Cytoskeletal Proteins genetics, Gene Expression, Hamartoma genetics, Humans, Peutz-Jeghers Syndrome genetics, Proliferating Cell Nuclear Antigen genetics, Tumor Suppressor Protein p53 genetics, beta Catenin, Cytoskeletal Proteins biosynthesis, Hamartoma metabolism, Peutz-Jeghers Syndrome metabolism, Proliferating Cell Nuclear Antigen biosynthesis, Trans-Activators, Tumor Suppressor Protein p53 biosynthesis
- Abstract
Objective: To study the significance of beta -catenin, p53 and PCNA in PJS harmatoma., Method: Paraffin sections of 29 hamartomas of 18 patient with Peutz-Jeghers syndrome, 19 patients with colorectal mucosa and 10 persons with normal mucosa were examined using LSAB. Positive cells were detected under light microscope., Results: All 10 persons with normal mucosa showed p53 negative and beta - catenin positive. The beta - catenin protein was predominantly localized to the plasma membrane. PCNA index (PI) in normal mucosa was 10.56 +/- 7.51. The PI of hamartoma and cancer was 44.57 +/- 21.15 and 32.96 +/- 18.88. The PI of the two groups was significantly higher than that of the normal group. In hamartoma, the positive cells were mainly located in the low 1/3 part of the mucous glands. The positive rate of p53 was 24.1% (7/29) in hamartomatouspolyps and 57.9% (11/19) in colorectal carcinoma (chi(2) = 5.581, P < 0.05). Abnormal expression rate of beta- catenin in colorectal carcinoma was 73.7% (14/19) and 41.3% (12/29) in hamartomatous polyps. Some epithelial cells showed nuclear localization of beta- catenin and concentration of cytoplasm. (chi(2) = 4.825, P < 0.05)., Conclusions: The proliferation activity of hamartomatous polyps increased significantly by multifactory interaction. p53 and beta- catenin play a role in the early stage of hamartoma- adenoma-carcinoma sequence but have a different mechanism in inducing colorectal cancer.
- Published
- 2002
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