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Abstract Background Greater yam is a key staple crop grown in tropical and subtropical regions, while its asexual propagation mode had led to non-flowering mutations. How transposable elements contribute to its genetic variations is rarely analyzed. We used transcriptome and whole genome sequencing data to identify active transposable elements (TEs) and genetic variation caused by these active TEs. Our aim was to shed light on which TEs would lead to its intraspecies variation. Results Annotation of de novo assembly transcripts indicated that 0.8 − 0.9% of transcripts were TE related, with LTR retrotransposons (LTR-RTs) accounted for 65% TE transcripts. A large portion of these transcripts were non-autonomous TEs, which had incomplete functional domains. The majority of mapped transcripts were distributed in genic deficient regions, with 9% of TEs overlapping with genic regions. Moreover, over 90% TE transcripts exhibited low expression levels and insufficient reads coverage to support full-length structure assembly. Subfamily analysis of Copia and Gypsy, the two LTR-RTs revealed that a small number of subfamilies contained a significantly larger number of members, which play a key role in generating TE transcript. Based on resequencing data, 15,002 L-RT insertion loci were detected for active LTR-RT members. The insertion loci of LTR-RTs were highly divergent among greater yam accessions. Conclusions This study showed the ongoing transcription and transpositions of TEs in greater yam, despite low transcription levels and incomplete proteins insufficient for autonomous transposition. While our research did not directly link these TEs to specific yam traits such as tuber yield and propagation mode, it lays a crucial foundation for further research on how these TE insertion polymorphisms (TIPs) might be related to variations in greater yam traits and its tuber propagation mode. Future research may explore the potential roles of TEs in trait variations, such as tuber yield and stress resistance, in greater yam.

Abstract Thermohaline staircases and mesoscale eddies play crucial roles in the transport of heat, salt, and nutrients in the ocean, yet their complex relationship remains unclear due to the limitations in observational resolution and lateral range. Here, utilizing high spatial resolution seismic images with a total length of 2518 km, we show the widespread occurrence of thermohaline staircases in the eastern Caribbean Sea and demonstrate their three-dimensional distribution. These staircases occupy about 70% of the total length of the seismic lines, with the remaining 30% occupied by six subsurface mesoscale eddies. These staircases are disrupted and interrupted due to the enhanced turbulent diffusivity driven by the vertical shear of these eddies. The numerous high-resolution seismic observations presented herein enhance our comprehension of the interplay between staircases and multiscale oceanic dynamical processes.

Abstract In pursuing cheap and effective oxygen reduction catalysts, the Fe/N/C system emerges as a promising candidate. Nevertheless, the structural transformations of starting materials into Fe- and N-doped carbon catalysts remains poorly characterized under pyrolytic conditions. Here, we explore the evolution of Fe species and track the formation of Fe–N4 site development by employing diverse in-situ diagnostic techniques. In-situ heating microscopy reveals the initial formation of FeOx nanoparticles and subsequent internal migration within the carbon matrix, which stops once FeOx is fully reduced. The migration and decomposition of nanoparticles then leads to carbon layer reconstruction. Experimental and theoretical analysis reveals size-dependent behavior of FeOx where nanoparticles below 7 nm readily release Fe atoms to form Fe–N4 while nanoparticles with sizes >10 nm tend to coalesce and impede Fe–N4 site formation. The work visualizes the pyrolysis process of Fe/N/C materials, providing theoretical guidance for the rational design of catalysts.

Abstract Objective Branchio-oto-renal syndrome (BOR, OMIM#113,650) is a rare autosomal dominant disorder that presents with a variety of symptoms, including hearing loss (sensorineural, conductive, or mixed), structural abnormalities affecting the outer, middle, and inner ear, branchial fistulas or cysts, as well as renal abnormalities.This study aims to identify the pathogenic variants by performing genetic testing on a family with Branchio-oto-renal /Branchio-otic (BO, OMIM#602,588) syndrome using whole-exome sequencing, and to explore possible pathogenic mechanisms. Methods The family spans 4 generations and consists of 9 individuals, including 4 affected by the BOR/BO syndrome. Phenotypic information, including ear malformation and branchial cleft, was collected from family members. Audiological, temporal bone imaging, and renal ultrasound examinations were also performed. Whole-exome sequencing was conducted to identify candidate pathogenic variants and explore the underlying molecular etiology of BOR/BO syndrome by minigene experiments. Results Intra-familial variability was observed in the clinical phenotypes of BOR/BO syndrome in this family. The severity and nature of hearing loss varied in family members, with mixed or sensorineural hearing loss. The proband, in particular, had profound sensorineural hearing loss on the left and moderate conductive hearing loss on the right. Additionally, the proband exhibited developmental delay, and her mother experienced renal failure during pregnancy and terminated the pregnancy prematurely. Genetic testing revealed a novel heterozygous variant NM_000503.6: c.639 + 3 A > C in the EYA1 gene in affected family members. In vitro minigene experiments demonstrated its effect on splicing. According to the American College of Medical Genetics (ACMG) guidelines, this variant was classified as likely pathogenic. Conclusion This study highlights the phenotypic heterogeneity within the same family, reports the occurrence of renal failure and adverse pregnancy outcomes in a female patient at reproductive age with BOR syndrome, and enriches the mutational spectrum of pathogenic variants in the EYA1 gene.

Seismic oceanography has been widely used in the study of internal solitary waves (ISWs) in recent years, and has achieved remarkable results. In this paper, we analyzed the multi-channel seismic reflection data in the Canterbury Basin offshore New Zealand from January 9 to January 29, 2000, collected by R/V Maurice Ewing. We observed 4 groups of ISWs (labeled ISW1s to ISW4s) on 4 seismic survey lines. We studied their waveforms and propagation speeds in detail. There are two theoretical structures used to describe the vertical waveform of ISWs: the first-order nonlinear vertical structure and the linear vertical structure. We found that ISW1s fit the nonlinear structure well, ISW3s and ISW4s fit the linear structure, and ISW2 does not fit either one. As the water depth increases, the waveforms of all ISWs gradually widen. Two satellite SAR images reveal that all ISWs generally travel shoreward across the isobaths. However, the propagation direction of ISW1s is about 354°-360° (clockwise from due north), different from the propagation directions of other ISWs (about 22°-26°), which explains why ISW1s have the largest characteristic half-height width. The estimated propagation speeds are close to the theoretical speeds, confirming our speed correction method. In the end, we also discuss the interaction of ISWs and eddies.

Abstract Efficient oxygen evolution reaction electrocatalysts are essential for sustainable clean energy conversion. However, catalytic materials followed the conventional adsorbate evolution mechanism (AEM) with the inherent scaling relationship between key oxygen intermediates *OOH and *OH, or the lattice-oxygen-mediated mechanism (LOM) with the possible lattice oxygen migration and structural reconstruction, which are not favorable to the balance between high activity and stability. Herein, we propose an unconventional Co-Fe dual-site segmentally synergistic mechanism (DSSM) for single-domain ferromagnetic catalyst CoFeS x nanoclusters on carbon nanotubes (CNT) (CFS-ACs/CNT), which can effectively break the scaling relationship without sacrificing stability. Co3+ (L.S, t 2g 6 e g 0) supplies the strongest OH* adsorption energy, while Fe3+ (M.S, t 2g 4 e g 1) exposes strong O* adsorption. These dual-sites synergistically produce of Co-O-O-Fe intermediates, thereby accelerating the release of triplet-state oxygen ( ↑ O = O ↑ ). As predicted, the prepared CFS-ACs/CNT catalyst exhibits less overpotential than that of commercial IrO2, as well as approximately 633 h of stability without significant potential loss.

Abiotic stresses pose a major increasing problem for the cultivation of maize. Autophagy plays a vital role in recycling and re-utilizing nutrients and adapting to stress. However, the role of autophagy in the response to abiotic stress in maize has not yet been investigated. Here, ZmATG3, which is essential for ATG8-PE conjugation, was isolated from the maize inbred line B73. The ATG3 sequence was conserved, including the C-terminal domains with HPC and FLKF motifs and the catalytic domain in different species. The promoter of the ZmATG3 gene contained a number of elements involved in responses to environmental stresses or hormones. Heterologous expression of ZmATG3 in yeast promoted the growth of strain under salt, mannitol, and low-nitrogen stress. The expression of ZmATG3 could be altered by various types of abiotic stress (200 mM NaCl, 200 mM mannitol, low N) and exogenous hormones (500 µM ABA). GUS staining analysis of ZmATG3-GUS transgenic Arabidopsis revealed that GUS gene activity increased after abiotic treatment. ZmATG3-overexpressing Arabidopsis plants had higher osmotic and salinity stress tolerance than wild-type plants. Overexpression of ZmATG3 up-regulated the expression of other AtATGs (AtATG3, AtATG5, and AtATG8b) under NaCl, mannitol and LN stress. These findings demonstrate that overexpression of ZmATG3 can improve tolerance to multiple abiotic stresses.

The earthquake and its secondary geological disasters have some long-term effects on vegetation recovery, and the spatiotemporal differentiation of vegetation coverage in earthquake-stricken areas are often not valued by researchers. Beichuan County in Sichuan Province is the worst-hit area during Wenchuan earthquake, tracking the vegetation coverage in this disaster area can provide feedback for post-earthquake ecological restoration. Based on remote sensing (RS) and Geographic information system (GIS), this paper relies on dimidiate pixel model (DPM) and vegetation coverage transfer matrix to explore the vegetation coverage of the earthquake worst-hit area. The results show that the overall level of vegetation coverage in Beichuan county is high, and earthquakes are dominant in the areas with high vegetation coverage. Besides, earthquake can do most severe damages to the areas with high vegetation coverage, followed by medium damage impacting to the areas with medium vegetation coverage, but the damage of other areas are relatively small. From 2007 to 2020, the average vegetation coverage in Beichuan County decreased from 0.835 in 2007 to 0.755 in 2008, then gradually recovered to 0.826 in 2020, experiencing a sharp decline and then a steady increase. The recovery of vegetation coverage is improved over time, but the spatiotemporal differences of the recovery process are obvious. Elevation has the largest driving force on vegetation coverage (q = 0.569, p less than 0.001), followed by temperature and rainfall; the interaction between factors has a significant increasement in the spatial differentiation of vegetation coverage, indicating that vegetation coverage is a combination result of multiple factors. The research findings can help construct the scientific management of the eco-environment in the disaster area, it can also help builda medium to long-term ecological restoration plan according to the variation in vegetation coverage.

Abstract Background In camels, nasopharyngeal myiasis is caused by the larvae of Cephalopina titillator, which parasitize the tissues of nasal and paranasal sinuses, pharynx, and larynx. C. titillator infestation adversely affects the health of camels and decreases milk and meat production and even death. However, the C. titillator infestation in Bactrian camels has not been widely studied. Methods The present study was conducted to determine the prevalence and risk factors of C. titillator in Bactrian camels of northwestern Xinjiang. Suspected larvae recovered from infested camels were evaluated for C. titillator by microscopy and polymerase chain reaction. Nucleotide sequences of the partial mitochondrial cytochrome c oxidase subunit I (COX1) and cytochrome b (CYTB) genes from the C. titillator of camels were aligned from the NCBI database. Furthermore, the gross and histopathological alterations associated with C. titillator infestation were evaluated via pathological examination. Results Of 1263 camels examined 685 (54.2%) camels were infested with suspected C. titillator larvae. Different larval stages were topically detected in the nasal passages and pharynx of the camel heads. Microscopy analysis of the pharyngeal mucosa tissue revealed necrotic tissue debris and some inflammatory cells. Molecular detection of the larval COX1 and CYTB genes indicated that pathogen collected in Bactrian camels was C. titillator. The epidemiological study demonstrated that the prevalence rate of C.titillator infestation was significantly higher in camels of Bestierek Town Pasture (67.2%) and Karamagai Town Pasture (63.6%) compared to Kitagel Town Pasture (38.7%) and Qibal Town Pasture (35.8%) (P 0.05). The prevalence was higher in warm (64.2%) than that in cold (48.4%) seasons (P 10 (61.1%) years old compared to those of aged

Abstract The carbon sequestration function of the ecosystem is one of the most important functions of ecosystem service, and it of great significance to study the spatio-temporal differentiation of carbon storage for promoting regional sustainable development. Ecosystems on the Western Sichuan Plateau are highly variable, but its spatio-temporal differentiation and driving factors are not yet clear. In this study, on the basis of land use monitoring data, meteorological and demographic data interpreted from Landsat remote sensing image, and through GIS analysis tools, the carbon storage module of InVEST (Integrated Valuation of Ecosystem Services and Trade-offs) model was used to estimate carbon storage and geodetector was used to detect the driving factors of carbon storage spatial differentiation. The results show that: (1) The carbon storage increased to 1.2455 × 1010 t from 1.2438 × 1010 t in the past 20 years, the ecosystem developed in a healthy way overall. (2) Carbon storage show High-High and Low-Low aggregation characteristics, but the area decreased by 1481.81 km2 and 311.11 km2 respectively, and the spatial cluster effect gradually weakened. (3) HAI is the leading factor causing the spatio-temporal differentiation of regional carbon storage, followed by temperature and NDVI; the interaction between factors significantly enhances the spatial differentiation of carbon storage, indicating that the change of carbon storage is the result of the joint action of natural and socioeconomic factors. The results of the study provide some theoretical basis for the development of differentiated ecological regulation models and strategies, and help to promote high-quality regional development.

Intensity-modulation direct-detection (IM/DD) probabilistically shaped (PS) 8-level pulse-amplitude modulation (PAM) signal is promising for high-speed intra-data-center networks. However, the challenges of a PS-PAM-8 transmission system are complicated distribution matcher (DM) and nonlinear equalizer. In this work, we propose a simple PS-PAM-8 transmission system, where the probabilistic amplitude shaping (PAS) encoder consists of an energy-level-assigned (ELA)-based DM and a low-density parity-check (LDPC) encoder. In the ELA-based DM, a simple lookup table is designed to realize an approximate bilateral Maxwell-Boltzmann distribution by assigning energy levels to various symbols. Consequently, the DM encoded bits are used as the amplitude bits, and DM label bits and LDPC check bits are designated as sign bits to implement PAS encoding. In addition, a simplified Volterra equalizer (SVE) is introduced to eliminate the inter-symbol interference and improve the receiver sensitivity of PS-PAM-8 signal, where the SVE only includes the 2-order kernel to reduce its complexity. We experimentally demonstrated the proposed PS-PAM-8 scheme and verified its outperformance over uniform PAM-8 signal in the back-to-back and 2-km standard single mode fiber (SSMF) transmission. Compared to the conventional linear equalizer, the SVE contributes approximate 1-dB improvement of the receiver sensitivity in the PS-PAM-8 2-km SSMF transmission, for the error-free performance of post forward error correction bit error ratio.

Objective: This study evaluated the associations of solid fuels with incidence of falls and fall-related injuries.Methods: Data were taken from wave 1∼4 of the China Health and Retirement Longitudinal Study, including 15,651 participants aged 45 years and older. Modified Poisson regression was used to examine the associations of solid fuels with falls and fall-related injuries.Results: Modified Poisson regression analysis showed that solid fuels users for cooking had an increasing incidence of falls and fall-related injuries, with RR of 1.211 (95% CI: 1.124, 1.305) and 1.248 (95% CI: 1.107, 1.408); for heating had an incidence, with RR of 1.178 (95% CI: 1.062, 1.306) and 1.134 (95% CI: 0.963, 1.335); combined for cooking and heating, with RR of 1.247 (95% CI: 1.105, 1.408) and 1.185 (95% CI: 0.982, 1.431).Conclusion: Our study suggests that solid fuel use is associated with a higher incidence of falls and fall-related injuries among adults aged 45 years and older in China. It is necessary to restrict solid fuel use to reduce household air pollution and make stronger environmental protection policies to improve household environment.

Platycodon grandiflorus, a perennial flowering plant widely distributed in China and South Korea, is an excellent resource for both food and medicine. The main active compounds of P. grandiflorus are triterpenoid saponins. WRKY transcription factors (TFs) are among the largest gene families in plants and play an important role in regulating plant terpenoid accumulation, physiological metabolism, and stress response. Numerous studies have been reported on other medicinal plants; however, little is known about WRKY genes in P. grandiflorus. In this study, 27 PgWRKYs were identified in the P. grandiflorus transcriptome. Phylogenetic analysis showed that PgWRKY genes were clustered into three main groups and five subgroups. Transcriptome analysis showed that the PgWRKY gene expression patterns in different tissues differed between those in Tongcheng City (Southern Anhui) and Taihe County (Northern Anhui). Gene expression analysis based on RNA sequencing and qRT-PCR analysis showed that most PgWRKY genes were expressed after induction with methyl jasmonate (MeJA). Co-expressing PgWRKY genes with triterpenoid biosynthesis pathway genes revealed four PgWRKY genes that may have functions in triterpenoid biosynthesis. Additionally, functional annotation and protein-protein interaction analysis of PgWRKY proteins were performed to predict their roles in potential regulatory networks. Thus, we systematically analyzed the structure, evolution, and expression patterns of PgWRKY genes to provide an important theoretical basis for further exploring the molecular basis and regulatory mechanism of WRKY TFs in triterpenoid biosynthesis.

Based on emotions as social information (EASI) theory, the current study proposed how and when patient gratitude expression could promote nurses’ innovative performance. Using a time-lagged data of 649 nurses from three class A tertiary hospitals in China, the results showed that patient gratitude expression was positively related to nurses’ innovative performance, and nurses’ work meaningfulness mediated such effect. Furthermore, supervisory support moderated the relationship of work meaningfulness with nurses’ innovative performance, as well as the indirect relationship between patient gratitude expression and innovative performance through work meaningfulness, such that the indirect relationship was stronger when supervisory support is higher. Our research helps to expand our understanding of how patient gratitude expression as an organizational external factor influences nurses’ innovation in healthcare, and meanwhile, provides management insights for hospital managers to focus on patient gratitude expression and enhance nurse innovation.

Biofilm-associated infections exert more severe and harmful attacks on human health since they can accelerate the generation and development of the antibiotic resistance of the embedded bacteria. Anti-biofilm materials and techniques that can eliminate biofilms effectively are in urgent demand. Therefore, we designed a type I photosensitizer (TTTDM) with an aggregation-induced emission (AIE) property and used F-127 to encapsulate the TTTDM into nanoparticles (F-127 AIE NPs). The NPs exhibit highly efficient ROS generation by enhancing intramolecular D–A interaction and confining molecular non-radiative transitions. Furthermore, the NPs can sufficiently penetrate the biofilm matrix and then detect and eliminate mature bacterial biofilms upon white light irradiation. This strategy holds great promise for the rapid detection and eradication of bacterial biofilms.

Q-switched lasers have occupied important roles in industrial applications such as laser marking, engraving, welding, and cutting due to their advantages in high pulse energy. Here, SnS2-based Q-switched lasers are implemented. Considering that SnS2 inherits the thickness sensitive optical characteristics of TMD, three kinds of SnS2 with different thickness are characterized in terms of nonlinearity and used to realize the Q-switched pulses under consistent implementation conditions for comparison tests. According to the results, the influence of thickness variation on the nonlinear performance of saturable absorber, such as modulation depth and absorption intensity, and the influence on the corresponding laser are analyzed. In addition, compared with other traditional saturable absorbers, the advantage of SnS2 in realizing ultrashort pulses is also noticed. Our work explores the thickness-dependent nonlinear optical properties of SnS2, and the rules found is of great reference value for the establishment of target lasers.

The outbreak and spread of novel coronavirus disease 2019 (COVID-19) with pandemic features, which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have greatly threatened global public health. Given the perniciousness of COVID-19 pandemic, acquiring a deeper understanding of this viral illness is critical for the development of new vaccines and therapeutic options. In this review, we introduce the systematic evolution of coronaviruses and the structural characteristics of SARS-CoV-2. We also summarize the current diagnostic tools and therapeutic strategies for COVID-19.

Abstract Due to the special working wavelength and superb beam quality, the mid‐infrared fiber laser has a wide application prospect in the fields of biomedicine, military defense, laser manufacturing, atmospheric detection, space communication, etc. In recent years, with the emergence of two‐dimensional (2D) saturable absorption materials, the development of mid‐infrared lasers ushered in new opportunities. In particular, as a potential 2D material, transition metal dichalcogenides (TMDs) with various types has attracted extensive attention from researchers because of its unique structural characteristics and ideal photoelectric performance. In this paper, the preparation, main properties and saturable absorption of TMDs are introduced in detail, and latest research progress of mid‐infrared lasers based on saturable absorbers at home and abroad is described.

Abstract High dosage of expensive Pt to catalyze the sluggish oxygen reduction reaction (ORR) on the cathode severely impedes the commercialization of proton exchange membrane fuel cells. Therefore, it is urgent to cut down the Pt catalyst by efficiently improving the ORR activity while maintaining high durability. Herein, magic concave Pt–Zn nanocubes with high‐index faceted Pt skin (Pt78Zn22) are proposed for high‐efficiency catalysis toward proton exchange membrane fuel cells. These unique structural features endow the Pt‐skin Pt78Zn22/KB with a mass activity of 1.18 mA μgPt–1 and a specific activity of 3.64 mA cm–2 for the ORR at 0.9 V (vs RHE). Meanwhile, the H2–O2 fuel cell assembled by this catalyst delivers an ultrahigh peak power density of ≈1449 mW cm–2. Both experiments and theoretical calculations show that the electronic structure of the surface is adjusted, thereby shortening the length of the Pt–Pt bond and reducing the adsorption energy of OH*/O* on the Pt surface. This work demonstrates the synergistic effect of the oxidation‐resistant metal Zn and the construction of Pt‐rich surface engineering. Also, it guides the future development of catalysts for their practical applications in energy conversion technologies and beyond.

Being closely related to ecosystem services, land use changes can affect ecosystem service functions by acting on ecosystem patterns and its processes, thus having impact on regional sustainable development. Taking the Western Sichuan Plateau as the study area based on land use data from 1990 to 2020, the CA-Markov model was employed to predict the land use pattern under natural change scenarios, and the improved InVEST model and equivalent method were used to evaluate the ecosystem services in the study area from two different perspectives, as to analyze the evolution trend. The results show that: 1) From 1990 to 2020, the carbon storage in the Western Sichuan Plateau showed a fluctuating increase, with an average annual increase of 3.17 × 107 t, of which the total contribution rate of woodland and grassland to the total carbon storage exceeded 95%. In recent 30 years, the carbon storage of the Western Sichuan Plateau has little spatial variation, with the increase and decrease regions accounting for less than 5% with overall sporadically dots-like distribution. 2) Ecosystem Service Value (ESV) increased by 1.36 × 1010 yuan in the past 30 years, which was similar to carbon storage and showed a wave-like increase; the contribution rate of woodland to the total ESV of the study area was more than 68%, occupying the dominant position. ESV was generally stable in the past 30 years, and the area with significant changes accounted for less than 9%, mainly located in the northeast and southwest of the study area. 3) In the next 10 years, under the current ecological protection measures, the carbon storage and ESV of the Western Sichuan Plateau will increase by 5.3980 × 107 t and 2.4087 × 109 yuan compared with that of 2020, indicating a positive development of ecosystem service function. This study results provides a scientific basis for the adjustment of land use structure and the management decision of ecological environment protection in the Western Sichuan Plateau.

Context Er Miao San (EMS) is a traditional Chinese medicine composed of Atractylodis Rhizoma and Phellodendri Cortex in a 1:1 weight ratio. EMS has been used to treat rheumatism in China for many years. Objective To evaluate the anti-arthritic activity of EMS extract on adjuvant-induced arthritis (AA) in Sprague-Dawley rats and to clarify its mechanisms of action. Materials and methods EMS (0.75, 1.5 and 3 g/kg, once daily) was orally administered from day 18 after immunization to day 31. The effects of EMS on AA rats were evaluated by histopathological examination, paw swelling and polyarthritis index. The proliferation of fibroblast-like synoviocyte (FLS) and T cells was detected by CCK-8. The percentages of Th17 cells and Treg cells in splenocytes were determined by flow cytometry. Levels of cytokines in serum were detected by ELISA. Results EMS treatment significantly decreased the paw volume (from 1.20 to 0.81), polyarthritis index (from 9.56 to 4.46) and alleviated ankle joint histopathology in AA rats. EMS inhibited the proliferation of FLS and T cells. Furthermore, EMS treatment decreased Th17 cells (from 4.62 to 2.08%) and increased Treg cells (from 2.77 to 4.75%) in splenocytes. The levels of IL-17A, TNF-α and IL-6 were remarkably decreased in the serum of EMS-treated rats, whereas the levels of IL-10 and TGF-β1 were significantly increased. Conclusions EMS exhibits anti-arthritic activity in the AA model by regulating the balance of cytokines and the ratio of Th17 and Treg cells. These insights may provide an experimental basis for the clinical treatment of RA.

The effect of interfacial oxygen migration on the thermal stability of the perpendicular magnetic anisotropy (PMA) in bottom and top structures was investigated in detail. By controlling the diffusion of thermally activated oxygen atoms, PMA with high thermal stability was achieved in the top structure. Compared with the bottom structure, the range of annealing temperature for which PMA was observed was extended to higher temperature (60 °C higher) via the strong Fe–O bonding in the top structure. Through detailed x-ray photoelectron spectroscopy analyses, the chemical states of different elements in CoFeB/MgO layers and the oxygen atom diffusion were investigated to understand the mechanisms behind the obtained high thermal stability of the PMA. It was found that the absence of thermally activated oxygen atom migration in the top structure is the main reason for the high thermal stability of the PMA. This study provides a promising way to obtain PMA with high thermal stability in CoFeB–MgO-based spintronic devices, which is significant to improve the compatibility of magnetic memories with the semiconductor integrated technology.

The GJB2 gene is the most common gene responsible for hearing loss (HL) worldwide, and missense variants are the most abundant type. GJB2 pathogenic missense variants cause nonsyndromic HL (autosomal recessive and dominant) and syndromic HL combined with skin diseases. However, the mechanism by which these different missense variants cause the different phenotypes is unknown. Over 2/3 of the GJB2 missense variants have yet to be functionally studied and are currently classified as variants of uncertain significance (VUS). Based on these functionally determined missense variants, we reviewed the clinical phenotypes and investigated the molecular mechanisms that affected hemichannel and gap junction functions, including connexin biosynthesis, trafficking, oligomerization into connexons, permeability, and interactions between other coexpressed connexins. We predict that all possible GJB2 missense variants will be described in the future by deep mutational scanning technology and optimizing computational models. Therefore, the mechanisms by which different missense variants cause different phenotypes will be fully elucidated.

Abstract Understanding the relationship between the electronic state of active sites and N2 reduction reaction (NRR) performance is essential to explore efficient electrocatalysts. Herein, atomically dispersed Fe and Mo sites are designed and achieved in the form of well‐defined FeN4 and MoN4 coordination in polyphthalocyanine (PPc) organic framework to investigate the influence of the spin state of FeN4 on NRR behavior. The neighboring MoN4 can regulate the spin state of Fe center in FeN4 from high‐spin (dxy2dyz1dxz1dz21dx2−y21) to medium‐spin (dxy2dyz2dxz1dz21), where the empty d orbitals and separate d electron favor the overlap of Fe 3d with the N 2p orbitals, more effectively activating N≡N triple bond. Theoretical modeling suggests that the NRR preferably takes place on FeN4 instead of MoN4, and the transition of Fe spin state significantly lowers the energy barrier of the potential determining step, which is conducive to the first hydrogenation of N2. As a result, FeMoPPc with medium‐spin FeN4 exhibits 2.0 and 9.0 times higher Faradaic efficiency and 2.0 and 17.2 times higher NH3 yields for NRR than FePPc with high‐spin FeN4 and MoPPc with MoN4, respectively. These new insights may open up opportunities for exploiting efficient NRR electrocatalysts by atomically regulating the spin state of metal centers.

Cationic polymers are a class of promising carriers for nucleic acid delivery. However, the balance between biosafety and transfection efficiency is still a major obstacle for nucleic acid delivery. Accurate structural design can effectively evaluate the relationship between structural factors and nucleic acid delivery. Previously designed series of vectors optimized based on artificial amino acid Stp have been shown to effectively improve gene transfection. Here, a series of sequenced-defined oligomers containing hydrophobic oleic acid with different topologies, different charge densities and with or without crosslinking cystine domains were designed and synthesized for a better understanding of the structure-activity relationship. The oligomers were further mixed with either pDNA or siRNA to form polyplexes. The particle size, zeta potential, formation properties, in vitro stability and reductive responsiveness of the polyplexes were investigated. In addition, studies regarding the transfection efficiency, cellular uptake, cellular trafficking, cytotoxicity and hemolysis were performed. The resulting oligomers display general good complexation ability with pDNA and siRNA with enhanced stability and redox sensitivity. Some of the oligomers showed adequate or surpassed transfection efficiency compared with PEI. The polyplexes formed with oligomer #1 have good cellular uptake and endo-lysosome escape properties. In addition, all the oligomers and the polyplexes showed low cytotoxicity and hemolysis, and demonstrated high biocompatibility. In summary, the results evidenced that tuning the charge density combined with hydrophobic oleic acid and thiol crosslinking cystine improves the overall performance in delivering pDNA and siRNA. [ABSTRACT FROM AUTHOR]

Phytophthora blight caused by Phytophthora capsici is a devastating disease for melon plant. However, the underlying resistance mechanisms are still poorly understood. In this study, the transcriptome differences between the resistant ZQK9 and susceptible E31 at 0, 3, and 5 days post-inoculation (dpi) were identified by RNA-seq. A total of 1,195 and 6,595 differentially expressed genes (DEGs) were identified in ZQK9 and E31, respectively. P. capsici infection triggered massive transcript changes in the inoculated tissues. Genes related to plant defense responses were activated, which was reflected by a lot of up-regulated DEGs involved in pathogenesis-related (PR) genes, hormones biosynthesis and signal transduction, secondary metabolites biosynthesis and cell wall modification in resistant ZQK9. The dataset generated in this study may provide a basis for identifying candidate resistant genes in melon against P. capsici and lay a foundation for further research on the molecular mechanisms.

From July to September 2009, a set of multi-channel seismic data was collected in the northern shelf area of the South China Sea. After the data was processed, we observed a series of shoaling events on one of the survey lines, including high-frequency internal waves, high-mode nonlinear internal waves, and shear instability. Using theoretical results from previous numerical simulations and field observations, coupled with local temperature and salinity data, we analyzed their depth distribution, waveform characteristics, and formation mechanisms, and discussed the influence of seafloor topography and stratification on the shoaling of solitary internal waves. We estimated the mixing parameters of seawater using a parameterization scheme based on hydrographic data and seismic data, respectively. And we found that the diapycnal mixing caused by these shoaling events in the shelf area were about 3.5 times greater than those on the slope. Consequently, the fission of internal solitary waves and the induced shear instability serve as significant mechanisms for the energy dissipation of internal solitary waves at the slope and shelf of the South China Sea. Additionally, the high-frequency internal waves generated during shoaling might also have a crucial role in this process. [ABSTRACT FROM AUTHOR]

Abstract Enteromorpha prolifera (E. prolifera), one of the main algae genera for green tide, significantly influences both the coastal ecological environment and seawater quality. How to effectively utilize this waste as reproducible raw resource with credible application mechanism are urgent environmental issues to be solved. Herein, E. prolifera was converted to attractive fluorescent carbon nanodots (CNDs) by one-pot green hydrothermal process. The purity and quantum yields for the as-prepared CNDs can be enhanced upon the post-treatment of ethanol sedimentation. The CNDs can be well dispersed in aqueous medium with uniform spherical morphology, narrow size distribution and average size of 2.75 ± 0.12 nm. The ease synthesis and relatively high quantum yields of the CNDs make E. prolifera inexpensive benefit to the human and nature, such as applications in efficient cell imaging and fiber staining. Furthermore, it was discovered that the fluorescence intensity of the CNDs can be selectively quenched upon Fe3+ addition, which can be used for specific sensitive assay and removal of Fe3+ in aqueous medium. More importantly, it was reasonably proposed that the quenching was resulted from the synergistic effects of CNDs aggregation and Fe3+-CNDs charge-transfer transitions due to the coordination interactions between Fe3+ and the oxygenous groups on the CNDs.