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The engagement of the cerebellum during reading tasks is not unprecedented. However, it is still unclear which regions in the cerebellum are specifically involved in reading and how the cerebellum processes different languages. With functional magnetic resonance imaging, we compared the cerebellar neural activity in Chinese child learners of English between reading and non-reading tasks to identify functionally specialized areas for reading, and between Chinese characters and English words in a passive viewing paradigm to detect regions sensitive to different scripts. Two posterior subregions of right lobule VI, as well as right lobule VIIIA, demonstrated greater activation to viewing Chinese characters and English words compared to the non-reading tasks. However, we did not find any cerebellar regions that were differentially responsive to Chinese versus English print. Instead, we observed that functional connectivity between the two above-mentioned cerebellar regions (lobules VI and VIIIA) and the left inferior parietal lobule was significantly greater in English reading compared to Chinese reading. Overall, these results indicate that the posterior parts of right lobule VI and the right lobule VIIIA could be reading-specific regions, and deepen our understanding of how the cerebellum contributes to reading.

Comparisons across adults with different sensory histories (blind vs. sighted) have uncovered effects of experience on the development of human brain function. In people born blind visual cortices become responsive to non-visual tasks and show enhanced functional coupling with fronto-parietal executive systems at rest. Little is known about the developmental origins of experience-based plasticity in humans, since almost all research has been done with adults. Here we take a new approach by comparing resting state data across blind (n=30) and blindfolded sighted (n=50) adults to two large cohorts of sighted infants (dHCP, n=327, n=475). By comparing the infant “starting state” to adult outcomes, we dissociate the instructive role of vision from reorganization due to blindness. As previously reported, we find that in sighted adults, visual networks show stronger functional coupling with other sensory-motor networks (i.e., auditory, somatosensory) than with higher-cognitive prefrontal networks at rest. By contrast, visual cortices of adults born blind show the opposite pattern: stronger functional connectivity with higher-cognitive prefrontal networks. Remarkably, we find that the connectivity profile of secondary visual cortices in infants resembles that of blind more than sighted adults. Visual experience appears to ‘instruct’ coupling of visual cortex with other sensory-motor networks and de-couple from prefrontal systems. By contrast primary visual cortex (V1) shows a mixture of instructive effects of vision and reorganizing effects of blindness. Finally, lateralization of occipital connectivity appears to be driven by blindness-related reorganization, since infants resembles sighted adults. These results reveal instructive and reorganizing effect of experience on functional connectivity of human cortex.

Non-coding RNAs are a class of RNAs, including circRNA and miRNA, that cannot be translated into proteins, but play an important role in the regulation of the expression of protein-coding genes. More and more evidences showed that circRNA can regulate the expression of miRNA target genes by adsorbing miRNA and form the circRNA-miRNA-mRNA regulatory network. The inactivated Aeromonas salmonicida vaccine is a commercial vaccine for many teleost. Understanding the role of circRNA and miRNA in the early stage of vaccine injection will provide a new insight for the study of the early immune response process in teleost. In this study, the expression profiles of circRNA, miRNA and mRNA were analyzed by high-throughput sequencing at 6 h, 12 h, 24 h and 96 h after injection of inactivated Aeromonas salmonicida vaccine and normal turbot spleen. Compared with the control group, 111, 141 and 453 differentially expressed circRNAs, miRNAs and mRNAs were identified in the four vaccination groups, respectively. The targeting relationships of differentially expressed miRNA to circRNA and mRNA were predicted by using miRanda software, and the results showed that a variety of differentially expressed immune-related genes were targeted. A total of 53 differentially expressed circRNA-miRNA-mRNA regulatory networks were constructed according to circRNA-miRNA pairs and miRNA-mRNA pairs. Among them, cell adhesion molecule 3 and immunoglobulin superfamily member 21 were regulated by the same miRNA (novel_880) and circRNA (novel_circ_0000311/novel_circ_0005326). These suggest that these circRNA-miRNA-mRNA regulatory networks may be a multi-molecule regulatory network, and its regulatory mechanism needs to be further studied.

Inorganic solid-state electrolytes (ISSEs) have been extensively researched as the critical component in all-solid-state lithium-metal batteries (ASSLMBs). Many ISSEs exhibit high ionic conductivities up to 10

Rechargeable lithium-based batteries built with high-energy anode materials (e.g., silicon-based and silicon-derivative materials) are considered a feasible solution to satisfy the stringent requirements imposed by emerging markets, including electric vehicles and grid storage, due to their higher energy density compared to contemporary lithium-ion batteries. The robustness of the solid electrolyte interphase (SEI) layer on high-energy anodes is critical to achieve long-term and stable cycling performances of the batteries. Herein, we propose a new type of designer cathode additive (DCA), i.e., an ultrathin coating layer of elemental sulfur on the cathode, for the in situ formation of a thin and robust SEI layer on various types of high-energy anodes. The DCA elemental sulfur undergoes simultaneous oxidation and reduction paths, forming lithium alkyl sulfate (R-OSO

Galectins, a family of evolutionary conserved β-galactoside-binding proteins, have been characterized in a wide range of species. Many reports have indicated vital roles of galectins in innate immunity, especially in the mucosal tissues against infection. However, the systematic identification of galectin gene family is still lacking in teleost. Here, we characterized the galectin gene family and investigated their expression profiles post bacterial challenge in turbot (Scophthalmus maximus L.). In this study, a total of 13 galectin genes were characterized in turbot, phylogenetic analyses revealed their strong relationships to half smooth tongue sole and puffer fish, and syntenic analyses confirmed the orthology suggested by the phylogenetic analysis. In addition, the copy number of galectin genes is similar across a broad spectrum of species from fish to amphibians, birds, and mammals, ranging from 8 to 16 genes. Furthermore, the galectin genes were widely expressed in all the examined turbot tissues, and most of the galectin genes were strongly expressed in mucosal tissues (skin, gill and intestine). Moreover, majority of the galectin genes were significantly regulated after Vibrio anguillarum infection in the intestine, gill and skin, suggesting that galectins were involved in the mucosal immune response to V. anguillarum infection in turbot. In addition, subcellular localization analysis showed lgals3a was distributed in the cytoplasm and nucleus. However, the knowledge of galectins are still limited in teleost species, further studies should be carried out to better characterize its detailed roles in teleost mucosal immunity.

In this paper, we investigate the known operator inequalities for the p-Schatten norm and obtain some refinements of these inequalities when parameters taking values in different regions. Let A1,...,An, B1,..., Bn ? Bp(H) such that ?ni,j=1Ai*Bj=0. Then p ? 2, p ? ? and ? ? 2, 21/p-?/4n3/p-?/4-1/2(?ni=1 ||Ai||4/?p + ?ni=1 ||Bi||4/?p )?/4 ? n2/p-1/2||?ni=1 |Ai|2 + ?ni=1 |Bi|2||1/2 p/2 ? n2/p-2/?(?ni,j=1 ||Ai ? Bj||?p)1/?. For 0 < p ? 2, p ? ? > 0 and 0 < ? ? 2, the inequalities are reversed. Moreover, we get some applications of our results.

Cathepsin L (CTSL) is one of the crucial enzymes in cathepsin family, which has been widely known for its involvement in the innate immunity. However, it still remains poorly understood how CTSL modulates the immune system of teleosts. In this study, we captured three cathepsin L genes (SmCTSL, SmCTSL.1 and SmCTSL1) from turbot (Scophthalmus maximus). The coding sequences of SmCTSL, SmCTSL.1 and SmCTSL1 are 1,026 bp, 1,005 bp and 1,017 bp in length and encode 341, 334 and 338 amino acids, respectively. In details, transcripts of CTSL genes share same domains as other CTSL genes, one signal peptide, one propeptide and one papain family cysteine protease domain. Protein interaction network analysis indicated that turbot CTSL genes may play important roles in apoptotic signaling and involve in innate immune response. Evidence from subcellular localization demonstrated that the three Cathepsin L proteins were ubiquitous in nucleus and cytoplasm. The cathepsin L genes were widely expressed in all the tested tissues with the highest expression level of SmCTSL in spleen, and SmCTSL.1 and SmCTSL1 in intestine. Following Vibrio anguillarum, Edwardsiella tarda and Streptococcus iniae challenge, these cathepsin L genes were significantly regulated in mucosal tissues in all the challenges, especially significant down-regulation occurred rapidly in intestine in all the three challenges. In addition, the three cathepsin L genes showed strong binding ability to all the examined microbial ligands (LPS, PGN and LTA). Further studies should be used to analyze the specific function of these three cathepsin L genes. By then, we can use their function to maintain the integrity of the mucosal barrier, thereby promoting the disease resistance line and family selection in turbot.

Many studies demonstrated that alphabetic language speaking children with developmental dyslexia had a deficit in visual-spatial attention, especially in rapid orienting of the attentional spotlight. Chinese, as a logographic language, is characterized as highly visual-spatial complexity. To date, few studies explored the visual-spatial attention of Chinese children with developmental dyslexia. The present study examined the visual-spatial attention of Chinese children with developmental dyslexia using the visual search task. The results showed that Chinese children with developmental dyslexia had poor performances in conjunction search, indicating that they had a deficit in the rapid orienting of visual-spatial attention. Meanwhile, only the conjunction search was a significant predictor of Chinese characters reading when other variables were controlled. These results indicated that Chinese dyslexic children had a deficit in visual-spatial attention, and visual-spatial attention played a special role in Chinese reading development.

The neural basis of reading is highly consistent across many languages and scripts. Are there alternative neural routes to reading? How does the sensory modality of symbols (tactile vs. visual) influence their neural representations? We examined these questions by comparing reading of visual print (sighted group, n=19) and tactile Braille (congenitally blind group, n=19). Blind and sighted readers were presented with written (words, consonant strings, non-letter shapes) and spoken stimuli (words, backward speech) that varied in word-likeness. Consistent with prior work, the ventral occipitotemporal cortex (vOTC) was active during Braille and visual reading. A posterior/anterior vOTC word-form gradient was observed only in sighted readers with more anterior regions preferring larger orthographic units (words). No such gradient was observed in blind readers. Consistent with connectivity predictions, in blind compared to sighted readers, posterior parietal cortices were recruited to a greater degree and contained word-preferring patches. Lateralization of Braille in blind readers was predicted by laterality of spoken language and reading hand. The effect of spoken language increased along a cortical hierarchy, whereas effect of reading-hand waned. These results suggested that the neural basis of reading is influenced by symbol modality and spoken language and support connectivity-based views of cortical function.

Increasing evidence suggests that non-coding RNAs (ncRNA) play an important role in a variety of biological life processes by regulating gene expression at the transcriptional and post-transcriptional levels. Turbot (Scophthalmus maximus) has been threatened by various pathogens. In this study, the expression of circular RNAs (circRNAs), microRNAs (miRNAs), and mRNA in the immune organs spleen of turbot infected with Aeromonas salmonicida was analyzed by high-throughput sequencing, and a circRNA-miRNA-mRNA network was constructed, so as to explore the function of non-coding RNA in the immune system of teleost. Illumina sequencing was performed on the uninfected group and infected group. A total of 119 differential expressed circRNAs (DE-circRNAs), 140 DE-miRNAs, and 510 DE-mRNAs were identified in the four infected groups compared with the uninfected group. Most DE-mRNAs and the target genes of DE-ncRNAs were involved in immune-related pathways. The quantitative real-time PCR (qRT-PCR) results verified the reliability and accuracy of the high-throughput sequencing data. Ninety-six differentially expressed circRNA-miRNA-mRNA regulatory networks were finally constructed. Among them, 15 circRNA-miRNA-mRNA were presented in the form of “up (circRNA)-down (miRNA)-up (mRNA)” or “down-up-down”. Immune-related genes gap junction CX32.2, cell adhesion molecule 3, and CC chemokine were also found in these networks. These results indicate that ncRNA may regulate the expression of immune-related genes through the circRNA-miRNA-mRNA regulatory network and thus participate in the immune response of turbot spleen after pathogen infection.

The neural basis of learning print-to-sound mapping when Chinese is the second language is still unclear. The present study aimed to examine the neural basis of the phonological consistency effect in non-native speakers who studied Chinese as a second language (L2 learners) for at least 1 year. The fMRI results indicated that the consistency effect occurred in the left supplementary motor area and left precentral gyrus in L2 learners, but not in native Chinese speakers, suggesting that L2 learners may utilize phonological-related regions and the motor system to facilitate inconsistent character processing. These results indicated that L2 learners used assimilation for the phonological processing of Chinese characters.

Galectins are a family of galactoside-binding proteins with an affinity for β-galactosides, involved in mediating fundamental processes including development, inflammation, cell migration and apoptosis. Galectin-4 is a member of tendem-repeat galectins, plays vital roles in intestinal epithelial barrier. Here, one galectin-4 gene was captured in turbot (SmLgals4) contains a 1197 bp open reading frame (ORF). In comparison to other species, SmLgals4 showed the highest similarity and identity both to large yellow croaker. The genomic structure analysis showed that SmLgals4 had conserved exons in the CRD domains compared to other vertebrate species. The syntenic analysis revealed that galectin-4 had the same neighboring genes across all the selected species, which suggested the synteny encompassing galectin-4 region during vertebrate evolution. Subsequently, SmLgals4 was widely expressed in all the examined tissues, with the highest expression level in intestine and the lowest expression level in skin. In addition, SmLgals4 was significantly down-regulated in intestine following both Gram-negative bacteria Vibrio anguillarum, and Gram-positive bacteria Streptococcus iniae immersion challenge. Finally, the rSmLgals4 showed strong binding ability to all the examined microbial ligands. Taken together, our results suggested SmLgals4 plays vital roles in fish intestinal immune responses against infection, but the detailed roles of galectin-4 in teleost are still lacking, further studies are needed to be carried out to characterize whether galectin-4 plays similar roles in teleost intestinal immunity.

The prawn, Litopenaeus vannamei (L. vannamei), is the most widely farmed species in the world but the incidence of enteritis in L. vannamei has increased in recent years. However, the pathogenesis of enteritis remains unclear. In this study, high-throughput sequencing was used to analyze the hepatopancreatic and intestinal transcriptome of healthy and enteritis-affected individuals from the same pond. In total, 1209 and 1608 differently-expressed genes (DEGs) were detected in the hepatopancreatic and intestinal transcriptomes, respectively. Significantly changed genes were enriched in the intestinal immune network for IgA Production, Lysosomes, Sphingolipid Metabolism and the Peroxisome Signaling Pathway. Expression of the integrin α4β7 gene was significantly increased in the intestine of L. vannamei with enteritis, while expression of 38 DEGs associated with the lysosome was significantly down-regulated. Furthermore, the expression of sphingolipid metabolism-related enzymes and superoxide dismutase (SOD) genes was also significantly decreased, indicating that abnormal autoimmune function, weak intestinal resistance to external pathogenic microbial invasion, and self-healing ability were important factors associated with enteritis in L. vannamei. In addition, the expression of trypsin and pancreatic lipase was decreased in the hepatopancreas of L. vannamei with enteritis. This study provided new insights into the possible molecular pathogenesis of enteritis in L. vannamei.

The engagement of the cerebellum in reading is not unprecedented. However, it is still unclear which regions in the cerebellum are specifically involved in reading and how the cerebellum processes different languages. With functional magnetic resonance imaging, we compared the cerebellar neural activity of Chinese child learners of English between reading and non-reading tasks to identify functionally specialized areas for reading, and between Chinese characters and English words in a passive viewing paradigm to detect regions sensitive to different scripts. We first focused on the right lobule VI, during which we observed two subregions in the posterior parts of right lobule VI showing greater activation in the reading tasks compared to the non-reading tasks. We then extended the target areas to the whole cerebellum, during which we observed another region, the right lobule VIIIA, demonstrating the same contrast pattern across tasks as the posterior parts of right lobule VI. However, we did not find any cerebellar regions showing significant language effects in terms of cerebellar activation. Instead, we observed that functional connectivity between the posterior part of right lobule VI and the left inferior parietal lobule was significantly greater in English reading compared to Chinese reading. Overall, these results indicate that the posterior part of right lobule VI and the right lobule VIIIA could be reading-specific regions. Even though activation in these two regions may not be able to differentiate scripts in a passive viewing condition, the right lobule VI contributed differently across languages by connecting with different cerebral regions. These findings deepen our understanding of how the cerebellum contributes to reading.

Cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, play multiple roles at physiological and pathological states. Cathepsin S is a lysosomal cysteine endopeptidase of the papain family, and exerts critical roles in the regulation of MHC class II immune responses. In the present study, we captured two Cathepsin S genes in turbot (SmCTSS1 and SmCTSS2.1), characterized their expression patterns following V. anguillarum and S. iniae infections, and explored their binding ability and agglutination capability. Firstly, the SmCTSS1 contained a 990 bp ORF encoding 329 amino acids, while SmCTSS2.1 contained a 1,014 bp ORF encoding 337 amino acids. The phylogenetic analysis revealed that both genes showed the closest relationship to their counterparts of Japanese flounder (Paralichthys olivaceus). In addition, both genes were ubiquitously expressed in all examined healthy tissues, with the highest expression level observed in spleen and intestine, respectively, while the lowest expression level both observed in liver. Both SmCTSS1 and SmCTSS2.1 were significantly differentially expressed, and exhibited general down-regulations at most time points in skin and intestine after two bacterial infections. Finally, both rSmCTSS1 and rSmCTSS2.1 showed significant binding ability to three examined microbial ligands (LPS, PGN and LTA), and strong agglutination effect to different bacteria (E. tarda, S. agalactiae, S. aureus and V. anguillarum). Collectively, this study provided valuable data for understanding the roles of CTSS in the host defense against bacterial infections in turbot, and indicated the potential vital roles of CTSS in innate immune responses of teleost species.

In this paper, we investigate some operator inequalities for the p-Schatten norm and obtain some other versions of these inequalities when parameters taking values in different regions. Let A1,…,An, B1,…,Bn∈BpH such that ∑i,j=1nAi∗Bj=0. Then, for p≥2, p≥λ>0, and 0<μ≤2, n21/p−1/λ∑i,j=1nAi±Bjpλ1/λ≤n1/2∑i=1nAi2+∑i=1nBi2p/21/2≤21/2−μ/4n1−μ/4∑i=1nAip4/μ+∑i=1nBip4/μμ/4. For 0

Scavenger receptors (SRs) are a group of membrane-bound receptors that could bind to a variety of ligands including endogenous proteins and pathogens. SRs have been recognized to play vital roles in innate immune response against pathogen infection in both vertebrates and invertebrates. In this regard, one SmSCARA5 gene was captured in turbot (Scophthalmus maximus). The full-length SmSCARA5 transcript contains an open reading frame (ORF) of 1494 bp. SmSCARA55 showed both the highest identity and similarity to half-smooth tongue sole (Cynoglossus semilaevis), and a high degree of conservation of genomic structure to the teleost species. In addition, the phylogenetic tree analysis showed SmSCARA5 had the closest relationship to half-smooth tongue sole, the syntenic analysis revealed a relatively conserved synteny pattern of SmSCARA5 to other species. Moreover, SmSCARA5 was ubiquitously expressed in all the examined tissues, with the highest expression level in brain and the lowest expression level in blood. And it was significantly down-regulated in intestine following Gram-negative bacteria Vibrio anguillarum, and Gram-positive bacteria Streptococcus iniae challenge. Finally, the recombinant SmSCARA5 showed the highest affinity to lipopolysaccharide (LPS), followed by peptidoglycan (PGN) and lipoteichoic acid (LTA), as well as the strong inhibition effect on the growth of V. anguillarum. Taken together, our results suggested SmSCARA5 plays vital roles in innate immune response in teleost, further studies should be carried out to better understand its regulatory mechanism for innate inflammation response in teleost.

Mucosal immune system is one of the most vital components in the innate immunity and constitutes the first line of host defense against bacterial infections, especially for the teleost, which live in the pathogen-rich aquatic environment. Cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, play multiple roles at physiological and pathological states. In this regard, we sought here to identify Cathepsin A in turbot (SmCTSA), characterize its mucosal expression patterns following Vibrio anguillarum and Streptococcus iniae infections in mucosal tissues, and explore its binding ability with three microbial ligands for the first time. The SmCTSA was 2631 bp long containing a 1422 bp open reading frame (ORF) that encoded 473 amino acids. Phylogenetic analysis revealed that SmCTSA showed the closest relationship to half-smooth tongue sole (Cynoglossus semilaevis). In addition, SmCTSA was ubiquitously expressed in all examined healthy tissues, with high expression levels in head kidney (HK) and intestine, while the lowest expression level in blood. Moreover, SmCTSA was significantly differentially expressed at least two timepoints in each mucosal tissue, suggesting its potential important roles in innate immune responses of turbot. Finally, in vitro assays showed that recombinant SmCTSA bound Lipopolysaccharide (LPS) with high affinity, and lipoteichoic acid (LTA) and peptidoglycan (PGN) with relatively low affinity. This study provides valuable data for understanding the roles of ctsa in the host defense against bacterial infections.

CD36 is a scavenger receptor, a type of membrane-bound receptors that characterized by recognizing a variety of ligands including endogenous proteins and pathogens. Here, we characterized CD36 gene in turbot, and its expression patterns in mucosal barriers following different bacterial infection, as well as microbial ligand binding ability and bacteriostatic activities. In current study, one SmCD36 gene was captured with a 1407 bp open reading frame (ORF). In multiple species comparison, SmCD36 showed the highest similarity and identity to Cynoglossus semilaevis. In the phylogenetic analysis, SmCD36 showed the closest relationship to C. semilaevis, followed by Takifugu rubripes. The genomic structure analysis showed that CD36 had 12 exons with almost the same length in vertebrate species, indicating the conservation of CD36 during evolution. The syntenic analysis revealed that CD36 located between GNAI1 and SEMA3C genes across all the selected species, which suggested the synteny encompassing CD36 region during vertebrate evolution. Subsequently, SmCD36 was expressed in all the examined turbot tissues, with the highest expression level in intestine. In addition, SmCD36 was significantly up-regulated in intestine following both Gram-negative bacteria Vibrio anguillarum, and Gram-positive bacteria Streptococcus iniae immersion challenge. Finally, the rSmCD36 showed strong binding ability to all the examined microbial ligands and significant inhibition effect on Staphylococcus aureusrequires. Taken together, our results suggested SmCD36 involved in fish innate immune responses to bacterial infection. However, the knowledge of CD36 are still limited in teleost species, further studies should be carried out to better characterize its detailed roles in teleost mucosal immunity.

Cathepsins are the best-known group of proteases in lysosomes, playing a significant role in immune responses. Cathepsin K (CTSK) is abundantly and selectively expressed in osteoclasts, dendritic cells and monocyte-derived macrophages, where it is involved in ECM degradation and bone remodeling. A growing body of evidences have indicated the vital roles of cathepsin K in innate immune responses. Here, one CTSK gene was captured in turbot (SmCTSK) with a 993 bp open reading frame (ORF). The genomic structure analysis showed that SmCTSK had 7 exons similar to other vertebrate species. The syntenic analysis revealed that CTSK had the same neighboring genes across all the selected species, which suggested the synteny encompassing CTSK region was conserved during vertebrate evolution. Subsequently, SmCTSK was widely expressed in all the examined tissues, with the highest expression level in spleen and the lowest expression level in liver. In addition, SmCTSK was significantly down-regulated in intestine following Gram-negative bacteria Vibrio anguillarum immersion challenge, but up-regulated in three tissues (gill, skin and intestine) following Gram-positive bacteria Streptococcus iniae immersion challenge. Finally, the rSmCTSK showed strong binding ability to all the examined microbial ligands. Taken together, our results suggested SmCTSK played vital roles in fish innate immune responses against infection. However, the knowledge of SmCTSK is still limited in teleost species, further studies should be carried out to better characterize its comprehensive roles in teleost mucosal immunity.

Previous neuroimaging studies have found atypical cerebellar activation in individuals with dyslexia in either motor-related tasks or language tasks. However, studies investigating atypical cerebellar activation in individuals with dyslexia have mostly used tasks tapping phonological processing. A question that is yet unanswered is whether the cerebellum in individuals with dyslexia functions properly during orthographic processing of words, as growing evidence shows that the cerebellum is also involved in visual and spatial processing. Here, we investigated cerebellar activation and cerebro-cerebellar functional connectivity during word processing in dyslexic readers and typically developing readers using tasks that tap orthographic and phonological codes. In children with dyslexia, we observed an abnormally higher engagement of the bilateral cerebellum for the orthographic task, which was negatively correlated with literacy measures. The greater the reading impairment was for young dyslexic readers, the stronger the cerebellar activation was. This suggests a compensatory role of the cerebellum in reading for children with dyslexia. In addition, a tendency for higher cerebellar activation in dyslexic readers was found in the phonological task. Moreover, the functional connectivity was stronger for dyslexic readers relative to typically developing readers between the lobule VI of the right cerebellum and the left fusiform gyrus during the orthographic task and between the lobule VI of the left cerebellum and the left supramarginal gyrus during the phonological task. This pattern of results suggests that the cerebellum compensates for reading impairment through the connections with specific brain regions responsible for the ongoing reading task. These findings enhance our understanding of the cerebellum's involvement in reading and reading impairment.

Previous studies have found that the orthographic representation of alphabetic languages is hierarchically organized from the posterior to the anterior parts of the left ventral occipitotemporal area (vOT). More word-like stimuli, such as real words and pseudo-words, induced higher activation in the middle and the anterior parts of the left vOT compared to false fonts or consonant letter strings, while different types of orthographic stimuli induced equal activation in the posterior part of the left vOT. In addition, such hierarchically tuning effect was not found in the right vOT. However, it remains unclear whether such a hierarchical organization can be generalized to the processing of logographic languages, such as Chinese. In the present study, we examined Chinese orthographic processing in the vOT with a passive view task using functional magnetic resonance imaging (fMRI). Similar to alphabetic languages, the posterior part of the vOT responds equivalently to different types of Chinese stimuli, including real characters, pseudo-characters, false characters, and stroke combination, indicating its role in general visual processing. Unlike alphabetic languages, however, we found that false characters induced stronger responses compared to real characters and pseudo-characters in the middle part of the left vOT as well as the middle part of the right vOT. These findings demonstrate that the features of orthographic organization in bilateral vOTs may differ between Chinese and alphabetic languages, suggesting that the functional organization of the vOTs is modulated by different orthographic conventions.

Galectins, a family of evolutionary conserved β-galactoside-binding proteins, have been characterized in a wide range of species. Galectin-3 is the only member in the chimera type, which is a monomeric lectin with one CRD domain. A growing body of evidence have indicated vital roles of galectin-3 in innate immune responses against infection. Here, one galectin-3 gene was captured in turbot (SmLgals3) with a 1203 bp open reading frame (ORF). In comparison to other species, SmLgals3 showed the highest similarity and identity to large yellow croaker and medaka, respectively. The genomic structure analysis showed that SmLgals3 had 5 exons similar to other vertebrate species. The syntenic analysis revealed that galectin-3 had the same neighboring genes across all the selected species, which suggested the synteny encompassing galectin-3 region during vertebrate evolution. Subsequently, SmLgals3 was widely expressed in all the examined tissues, with the highest expression level in brain and the lowest expression level in skin. In addition, SmLgals3 was significantly down-regulated in intestine following both Gram-negative bacteria Vibrio anguillarum, and Gram-positive bacteria Streptococcus iniae immersion challenge. Finally, the rSmLgals3 showed strong binding ability to all the examined microbial ligands. Taken together, our results suggested SmLgals3 played vital roles in fish innate immune responses against infection. However, the knowledge of SmLgals3 are still limited in teleost species, further studies should be carried out to better characterize its detailed roles in teleost mucosal immunity.

Several neuroimaging studies have explored the neural basis of literacy difficulties in the second language (L2). However, it remains unclear whether the associated neural alterations are related to literacy abilities in the first language (L1). Using magnetic resonance imaging, we explore this issue with two experiments in Mandarin-speaking children learning English as second language. In the first experiment, we investigated children with literacy difficulties in L2 and L1 (poor in both, PB) and children with literacy difficulties only in L2 (poor in English, PE). We compared the brain structure in these two groups to a control literacy (CL) group. The results showed that the CL group had significantly less gray matter volume in the left supramarginal gyrus compared to the PB group and moderately less gray matter volume compared to the PE group. In addition, the PB group had significant greater gray matter volume in the left medial fusiform gyrus compared to the PE group and had marginally greater gray matter volume compared to the CL group. In the second experiment, we explored the relationship between the two atypical regions and literacy abilities in the two languages in an independent sample consisting of children with typical literacy. Correlation analyses revealed that the left supramarginal gyrus was significantly associated with literacy performance only in the second language, English, whereas the left medial fusiform gyrus did not correlate with the performances in either L1 or L2. Taken together, these findings suggest that literacy difficulties in an alphabetic L2 are associated with a structural abnormality in the left supramarginal gyrus, a region implicated in phonological processing, which is independent of literacy abilities in the native language.

Climate changes show asymmetric warming, and warming is typically greater during night than day. Field experiments were conducted using two winter wheat cultivars, Yangmai 13 (YM13) and Yannong 19 (YN19), to investigate the effects of night warming during winter (Winter warming treatment, WT) and spring (Spring warming treatment, ST) seasons on physiological characters and grain yield of winter wheat ( Triticum aestivum L.) in the Yangtze River Basin of China in 2012–2014. The treatments of WT and ST were designed from tillering to jointing and from jointing to booting with mean night temperature increased as 1.56–1.67 °C and 1.78–1.92 °C, respectively. The WT treatment significantly enhanced grain yield by increasing grain number and 1000-grains weight for both cultivars, while the ST treatment had less impact than WT treatment. Both warming treatments shortened pre-anthesis while elongated post-anthesis growth duration, which resulted in higher post-anthesis growth degree days (GDD). The WT treatment significantly enhanced pre-anthesis crop growth rate (CGR), biomass and leaf area index (LAI) at anthesis, but the ST treatment showed slight increase as compared with the controls. Furthermore, the WT treatment increased net photosynthetic rate (Pn), maximum photochemical efficiency ( F v / F m ), actual photosynthetic efficiency ( Ф PSII ) and the contents of total soluble protein and ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in flag leaves within 21 days after anthesis. These results indicate that winter night warming treatment promotes pre-anthesis plant growth, leaf development and improves post-anthesis photosynthetic capacity eventually resulting in increased grain yield while spring night warming shows less impact on grain yield.

Auditory phonological processing skills are critical for successful reading development in English not only in native (L1) speakers but also in second language (L2) learners. However, the neural deficits of auditory phonological processing remain unknown in English-as-the-second-language (ESL) learners with reading difficulties. Here we investigated neural responses during spoken word rhyme judgments in typical and impaired ESL readers in China. The impaired readers showed comparable activation in the left superior temporal gyrus (LSTG), but reduced activation in the left inferior frontal gyrus (LIFG) and left fusiform and reduced connectivity between the LSTG and left fusiform when compared to typical readers. These findings suggest that impaired ESL readers have relative intact representations but impaired manipulation of phonology and reduced or absent automatic access to orthographic representations. This is consistent with previous findings in native English speakers and suggests a common neural mechanism underlying English impairment across the L1 and L2 learners.

Abundant anthocyanins accumulate in many flowers, leaves, fruits, as well as stems. In this study, an F2 segregating population derived from a cross between Chinese kale with a purple stem and kale with a green stem was used to decipher the genetic basis for the stem color. Bulked segregant analysis in combination with RNA sequencing (BSR-seq) was used to genetically map the causal gene for the purple stem (Ps), and the allele frequency difference between the two pools showed a single peak on chromosome C09. Ps was fine-mapped to the 0.32 cM interval with 406 kb sequences. A gene-encoding dihydroflavonol 4-reductase (DFR), an enzyme in the anthocyanin biosynthesis pathway, was differentially expressed between the two pools, and was identified as the candidate gene for the purple stem in Chinese kale. Sequence analysis revealed that 1 bp was inserted in the coding sequence of exon 2 of the BoDRF gene in green kale, causing frameshift and loss of function. The expression level of the BoDFR gene was significantly lower than the Chinese kale cultivar with a purple stem. The genetic results of the Ps gene will be useful for future development of purple vegetables.

The immune system protects organism from external pathogens, this progress starts with the pathogen recognition by pattern recognition receptors (PRRs). As a group of PRRs, the class B scavenger receptors showed important roles in phagocytosis. Among three class B scavenger receptors, lysosomal integral membrane protein type 2 (LIMP-2) was reported to present in the limiting membranes of lysosomes and late endosomes, but its immune roles in teleost species are still limited in handful species. Here, we characterized LIMP-2 gene in turbot, and its expression patterns in mucosal barriers following different bacterial infection, as well as ligand binding activities to different microbial ligands and agglutination assay with different bacteria. In our results, one SmLIMP2 gene was identified with a 1,593 bp open reading frame (ORF). The multiple species comparison and phylogenetic analysis showed the closest relationship to Paralichthys olivaceus, the genomic structure analysis and syntenic analysis revealed the conservation of LIMP-2 during evolution. In tissue distribution analysis, SmLIMP-2 was expressed in all the examined turbot tissues, with the highest expression level in brain, and the lowest expression level in liver. In addition, SmLIMP-2 was significantly up-regulated in all the mucosal tissues (skin, gill and intestine) following Gram-negative bacteria Vibrio anguillarum infection, and was only up-regulated in gill following Gram-positive bacteria Streptococcus iniae challenge. Finally, the rSmLIMP-2 showed strong binding ability to all the examined microbial ligands, and strong agglutination with Escherichia coli, Staphylococcus aureus and V. anguillarum. Taken together, our results suggested SmLIMP-2 played important roles in fish immune response to bacterial infection. However, further functional studies should be carried out to better characterize its detailed roles in teleost immunity.

Hydroponic experiments were conducted to investigate the effects of low nitrogen (N) nutrition on photosynthesis and its relationships with N status in wheat (Triticum aestivum L.). Two wheat cultivars, Zaoyangmai and Yangmai158, differing in low N nutrition tolerances, were used. The results show that under low N nutrition the area of the first top leaf was significantly reduced, while there was no significant difference in the top second and third leaf areas compared with the control for either cultivar. The net photosynthetic rate and chlorophyll content were significantly reduced in the top three leaves of Zaoyangmai, while no significant difference in these factors was observed in the top first and second leaves of Yangmai158 compared with control under N-limited conditions. The effective quantum yield of photosystem II (PSII) photochemistry and the maximal quantum yield of PSII photochemistry were only slightly altered in both cultivars, indicating that PSII was not damaged by low N nutrition. In addition, the non-photochemical quenching coefficient increased significantly in the top three leaves of Zaoyangmai, and only in the top third leaf of Yangmai158 under N-limited conditions. Furthermore, the ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and soluble protein contents decreased significantly in the top second and third leaves of Zaoyangmai, while no significant difference was observed in the top first and second leaves of Yangmai158 between low N nutrition and control. We concluded that in Yangmai158, N status changed less, and it maintained almost normal photosynthesis in young leaves, thus Yangmai158 could be more tolerance to low N nutrition.