Search

Your search keyword '"Meregalli, Chiara"' showing total 43 results
Start Over Author "Meregalli, Chiara"
43 results on '"Meregalli, Chiara"'

1. Tessuto adiposo epicardico: dagli aspetti biomolecolari alla pratica clinica

Author

Malavazos, Alexis Elias, Dubini, Carola, Meregalli, Chiara, Boniardi, Federico, Secchi, Francesco, Piepoli, Massimo, Salvetti, Guido, Menicanti, Lorenzo, Chessa, Massimo, Spagnolo, Pietro, Scravaglieri, Valentina, Trevisan, Manuel Bruno, Fierabracci, Paola, Dozio, Elena, Buscemi, Paolo, Castelvecchio, Serenella, Ruocco, Chiara, Randazzo, Cristiana, Morricone, Lelio, Santini, Ferruccio, Cinti, Saverio, Buscemi, Silvio, Guazzi, Marco, Romanelli, Massimiliano Marco Corsi, Carruba, Michele Olivo, and Nisoli, Enzo

Published
2024
Full Text
View/download PDF

2. The glucose and lipid metabolism reprogramming is gradedependent in clear cell renal cell carcinoma primary cultures and is targetable to modulate cell viability and proliferation

Author

Bianchi, Cristina, Meregalli, Chiara, Bombelli, Silvia, Di Stefano, Vitalba, Salerno, Francesco, Torsello, Barbara, De Marco, Sofia, Bovo, Giorgio, Cifola, Ingrid, Mangano, Eleonora, Battaglia, Cristina, Strada, Guido, Lucarelli, Giuseppe, Weiss, Robert H, and Perego, Roberto A

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Rare Diseases, Nutrition, Kidney Disease, Cancer, Affordable and Clean Energy, renal cell carcinoma, primary cell cultures, glucose and lipid metabolism reprogramming, Fuhrman grade, Oncology and Carcinogenesis, Oncology and carcinogenesis
Abstract

Clear cell renal cell carcinoma (ccRCC) has a poor prognosis despite novel biological targeted therapies. Tumor aggressiveness and poor survival may correlate with tumor grade at diagnosis and with complex metabolic alterations, also involving glucose and lipid metabolism. However, currently no grade-specific metabolic therapy addresses these alterations. Here we used primary cell cultures from ccRCC of low- and high-grade to investigate the effect on energy state and reduced pyridine nucleotide level, and on viability and proliferation, of specific inhibition of glycolysis with 2-deoxy-D-glucose (2DG), or fatty acid oxidation with Etomoxir. Our primary cultures retained the tissue grade-dependent modulation of lipid and glycogen storage and aerobic glycolysis (Warburg effect). 2DG affected lactate production, energy state and reduced pyridine nucleotide level in high-grade ccRCC cultures, but the energy state only in low-grade. Rather, Etomoxir affected energy state in high-grade and reduced pyridine nucleotide level in low-grade cultures. Energy state and reduced pyridine nucleotide level were evaluated by ATP and reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) dye quantification, respectively. 2DG treatment impaired cell proliferation and viability of low-grade ccRCC and normal cortex cultures, whereas Etomoxir showed a cytostatic and cytotoxic effect only in high-grade ccRCC cultures. Our data indicate that in ccRCC the Warburg effect is a grade-dependent feature, and fatty acid oxidation can be activated for different grade-dependent metabolic needs. A possible grade-dependent metabolic therapeutic approach in ccRCC is also highlighted.

Published
2017

3. The glucose and lipid metabolism reprogramming is grade-dependent in clear cell renal cell carcinoma primary cultures and is targetable to modulate cell viability and proliferation.

Author

Bianchi, Cristina, Meregalli, Chiara, Bombelli, Silvia, Di Stefano, Vitalba, Salerno, Francesco, Torsello, Barbara, De Marco, Sofia, Bovo, Giorgio, Cifola, Ingrid, Mangano, Eleonora, Battaglia, Cristina, Strada, Guido, Lucarelli, Giuseppe, Weiss, Robert H, and Perego, Roberto A

Subjects
Fuhrman grade, glucose and lipid metabolism reprogramming, primary cell cultures, renal cell carcinoma, Oncology and Carcinogenesis
Abstract

Clear cell renal cell carcinoma (ccRCC) has a poor prognosis despite novel biological targeted therapies. Tumor aggressiveness and poor survival may correlate with tumor grade at diagnosis and with complex metabolic alterations, also involving glucose and lipid metabolism. However, currently no grade-specific metabolic therapy addresses these alterations. Here we used primary cell cultures from ccRCC of low- and high-grade to investigate the effect on energy state and reduced pyridine nucleotide level, and on viability and proliferation, of specific inhibition of glycolysis with 2-deoxy-D-glucose (2DG), or fatty acid oxidation with Etomoxir. Our primary cultures retained the tissue grade-dependent modulation of lipid and glycogen storage and aerobic glycolysis (Warburg effect). 2DG affected lactate production, energy state and reduced pyridine nucleotide level in high-grade ccRCC cultures, but the energy state only in low-grade. Rather, Etomoxir affected energy state in high-grade and reduced pyridine nucleotide level in low-grade cultures. Energy state and reduced pyridine nucleotide level were evaluated by ATP and reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) dye quantification, respectively. 2DG treatment impaired cell proliferation and viability of low-grade ccRCC and normal cortex cultures, whereas Etomoxir showed a cytostatic and cytotoxic effect only in high-grade ccRCC cultures. Our data indicate that in ccRCC the Warburg effect is a grade-dependent feature, and fatty acid oxidation can be activated for different grade-dependent metabolic needs. A possible grade-dependent metabolic therapeutic approach in ccRCC is also highlighted.

Published
2017

4. BNT162b2 Booster Dose Elicits a Robust Antibody Response in Subjects with Abdominal Obesity and Previous SARS-CoV-2 Infection

Author

Malavazos, Alexis Elias, primary, Dubini, Carola, additional, Milani, Valentina, additional, Boveri, Sara, additional, Meregalli, Chiara, additional, Bertolini, Caterina, additional, Buscemi, Carola, additional, Cardani, Rosanna, additional, Renna, Laura Valentina, additional, Trevisan, Manuel Bruno, additional, Scravaglieri, Valentina, additional, Cuppone, Maria Teresa, additional, Menicanti, Lorenzo, additional, Costa, Elena, additional, Ambrogi, Federico, additional, Ruocco, Chiara, additional, Carruba, Michele, additional, Iacobellis, Gianluca, additional, Nisoli, Enzo, additional, and Corsi Romanelli, Massimiliano Marco, additional

Published
2023
Full Text
View/download PDF

6. Semaglutide therapy decreases epicardial fat inflammation and improves psoriasis severity in patients affected by abdominal obesity and type-2 diabetes

Author

Malavazos, Alexis Elias, primary, Meregalli, Chiara, additional, Sorrentino, Fabio, additional, Vignati, Andrea, additional, Dubini, Carola, additional, Scravaglieri, Valentina, additional, Basilico, Sara, additional, Boniardi, Federico, additional, Spagnolo, Pietro, additional, Malagoli, Piergiorgio, additional, Romanelli, Paolo, additional, Secchi, Francesco, additional, and Iacobellis, Gianluca, additional

Published
2023
Full Text
View/download PDF

7. BNT162b2 Booster Dose Elicits a Robust Antibody Response in Subjects with Abdominal Obesity and Previous SARS-CoV-2 Infection.

Author

Malavazos, Alexis Elias, Dubini, Carola, Milani, Valentina, Boveri, Sara, Meregalli, Chiara, Bertolini, Caterina, Buscemi, Carola, Cardani, Rosanna, Renna, Laura Valentina, Trevisan, Manuel Bruno, Scravaglieri, Valentina, Cuppone, Maria Teresa, Menicanti, Lorenzo, Costa, Elena, Ambrogi, Federico, Ruocco, Chiara, Carruba, Michele, Iacobellis, Gianluca, Nisoli, Enzo, and Corsi Romanelli, Massimiliano Marco

Subjects
BOOSTER vaccines, ANTIBODY formation, COVID-19 vaccines, ABDOMINAL adipose tissue, SARS-CoV-2
Abstract

Little is known about the long-term durability of the induced immune response in subjects with obesity, particularly in those with an abdominal distribution of adipose tissue. We evaluated SARS-CoV-2-specific antibody responses after BNT162b2 vaccine booster dose, comparing individuals with and without abdominal obesity (AO), discerning between individuals previously infected or not. IgG-TrimericS were measured in 511 subjects at baseline, on the 21st day after vaccine dose 1, and at 1, 3, 6, and 9 months from dose 2, and at 1 and 3 months following the booster dose. To detect SARS-CoV-2 infection, nucleocapsid antibodies were measured at baseline and at the end of the study. Multivariable linear regression evaluated the three-month difference in the absolute variation in IgG-TrimericS levels from booster dose, showing AO and SARS-CoV-2 infection status interactions (p = 0.016). Regardless of possible confounding factors and IgG-TrimericS levels at the booster dose, AO is associated with a higher absolute change in IgG-TrimericS in prior infected individuals (p = 0.0125). In the same regression model, no interaction is highlighted using BMI (p = 0.418). The robust response in the development of antibodies after booster dose, observed in people with AO and previous infection, may support the recommendations to administer a booster dose in this population group. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

8. High glucose induces an activated state of partial epithelial-mesenchymal transition in human primary tubular cell cultures

Author

Torsello, B, De Marco, S, Bombelli, S, Cifola, I, Morabito, I, Invernizzi, L, Meregalli, C, Zucchini, N, Strada, G, Perego, R, Bianchi, C, Torsello, Barbara, De Marco, Sofia, Bombelli, Silvia, Cifola, Ingrid, Morabito, Ivana, Invernizzi, Lara, Meregalli, Chiara, Zucchini, Nicola, Strada, Guido, Perego, Roberto A, Bianchi, Cristina, Torsello, B, De Marco, S, Bombelli, S, Cifola, I, Morabito, I, Invernizzi, L, Meregalli, C, Zucchini, N, Strada, G, Perego, R, Bianchi, C, Torsello, Barbara, De Marco, Sofia, Bombelli, Silvia, Cifola, Ingrid, Morabito, Ivana, Invernizzi, Lara, Meregalli, Chiara, Zucchini, Nicola, Strada, Guido, Perego, Roberto A, and Bianchi, Cristina

Abstract

Tubulointerstitial fibrosis is observed in diabetic nephropathy. It is still debated whether tubular cells, undergoing epithelial-mesenchymal transition (EMT) in high glucose (HG) conditions, may contribute to interstitial fibrosis development. In this study, we investigated the phenotypic and molecular EMT-like changes and the alteration of inflammatory and fibrogenic secretome induced by HG in human primary tubular cell cultures. Taking advantage of this in vitro cell model composed of proximal and distal tubular cells, we showed that HG-treated tubular cells acquired a fibroblast-like morphology with increased cytoplasmic stress fibers, maintaining the expression of the epithelial markers specific of proximal and distal tubular cells. HG increased Snail1, miRNA210 and Vimentin mesenchymal markers, decreased N-cadherin expression and migration ability of primary tubular cells, while E-cadherin expression and focal adhesion distribution were not affected. Furthermore, HG treatment of tubular cells altered the inflammatory cytokine secretion creating a secretome able to enhance the proliferation and migration of fibroblasts. Our findings show that HG promotes an activated state of partial EMT in human tubular primary cells and induces a pro-inflammatory and pro-fibrogenic microenvironment, supporting the active role of tubular cells in diabetic nephropathy onset.

Published
2023

10. High glucose induces an activated state of partial epithelial-mesenchymal transition in human primary tubular cell cultures

Author

Torsello, Barbara, primary, De Marco, Sofia, additional, Bombelli, Silvia, additional, Cifola, Ingrid, additional, Morabito, Ivana, additional, Invernizzi, Lara, additional, Meregalli, Chiara, additional, Zucchini, Nicola, additional, Strada, Guido, additional, Perego, Roberto A., additional, and Bianchi, Cristina, additional

Published
2023
Full Text
View/download PDF

11. Human Epicardial Adipose Tissue Expresses Glucose-dependent Insulinotropic Polypeptide, Glucagon and Glucagon-Like Peptide 1 Receptors as Potential Targets of Pleiotropic Therapies

Author

Malavazos, Alexis Elias, primary, Iacobellis, Gianluca, additional, Dozio, Elena, additional, Basilico, Sara, additional, Di Vincenzo, Angelica, additional, Dubini, Carola, additional, Menicanti, Lorenzo, additional, Vianello, Elena, additional, Meregalli, Chiara, additional, Ruocco, Chiara, additional, Ragni, Maurizio, additional, Secchi, Francesco, additional, Spagnolo, Pietro, additional, Castelvecchio, Serenella, additional, Morricone, Lelio, additional, Buscemi, Silvio, additional, Giordano, Antonio, additional, Goldberger, Jeffrey J, additional, Carruba, Michele, additional, Cinti, Saverio, additional, Corsi Romanelli, Massimiliano Marco, additional, and Nisoli, Enzo, additional

Published
2023
Full Text
View/download PDF

12. Retraction: Arg tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high-glucose conditions

Author

Torsello, Barbara, primary, Bianchi, Cristina, additional, Meregalli, Chiara, additional, Stefano, Vitalba Di, additional, Invernizzi, Lara, additional, Marco, Sofia De, additional, Bovo, Giorgio, additional, Brivio, Rinaldo, additional, Strada, Guido, additional, Bombelli, Silvia, additional, and Perego, Roberto A., additional

Published
2020
Full Text
View/download PDF

13. PKHhigh/CD133+/CD24− Renal Stem-Like Cells Isolated from Human Nephrospheres Exhibit In Vitro Multipotency

Author

Bombelli, Silvia, primary, Meregalli, Chiara, additional, Grasselli, Chiara, additional, Bolognesi, Maddalena M., additional, Bruno, Antonino, additional, Eriani, Stefano, additional, Torsello, Barbara, additional, De Marco, Sofia, additional, Bernasconi, Davide P., additional, Zucchini, Nicola, additional, Mazzola, Paolo, additional, Bianchi, Cristina, additional, Grasso, Marco, additional, Albini, Adriana, additional, Cattoretti, Giorgio, additional, and Perego, Roberto A., additional

Published
2020
Full Text
View/download PDF

14. STUDY OF MULTIPOTENT RENAL PKHHIGH STEM-LIKE CELLS, ISOLATED FROM HUMAN NEPHROSPHERES: REGENERATIVE ABILITIES AND TRANSCRIPTOMIC PROFILE

Author

MEREGALLI, CHIARA, Meregalli, C, and PEREGO, ROBERTO

Subjects
Differentiation, MED/04 - PATOLOGIA GENERALE, StemCell, Kidney, Signature, Decellularization
Abstract

Il meccanismo alla base della riparazione di un danno a cellule renali è ancora oggi oggetto di dibattito e potrebbe coinvolgere sia cellule terminalmente differenziate che l'esistenza di cellule staminali multipotenti quiescenti. Sfruttando il saggio di formazione di sfere e la tecnica del FACS sorting, il nostro gruppo ha isolato una popolazione di cellule marcate col clorante PKH26 che avessero caratteristiche di cellule staminali renali adulte (PKHhigh). In precedenza abbiamo valutato la capacità di queste cellule di differenziare in vitro in lineage epiteliale, podocitico ed endoteliale. Abbiamo anche dimostrato che la popolazione PKHhigh è eterogenea nella sua composizione e all'interno delle nefrosfere le cellule con capacità simil-staminali sono PKHhigh/CD133+/CD24- (RSC). Abbiamo recentemente pubblicato che le nostre cellule delle nefrosfere, che comprendono cellule PKHhigh e la loro progenie PKHlow/neg, sono in grado di ripopolare scaffold renali umani decellularizzati. Con questa ricerca, ora abbiamo come scopo: i) di dimostrare le capacità rigenerative delle cellule staminali PKHhigh, anche in assenza della loro progenie PKHlow/neg. ii) di trovare la signature molecolare delle RSC che, tra PKHhigh, sono le cellule con capacità staminali più ampie. Per raggiungere questi obiettivi, abbiamo messo in coltura cellule PKHhigh su scaffold acellulari per 30 giorni e le cellule delle strutture ripopolate sono state caratterizzate mediante immunofluorescenza sequenziale, utilizzando specifici marcatori di differenziamento. Alcune strutture hanno indicato un differenziamento terminale in lineage epiteliale tubulare prossimale o distale o in quello endoteliale. Solo poche strutture mostravano invece la coespressione di alcuni o di tutti i marcatori testati, suggerendo un fenotipo ancora immaturo. Per la comprensione della signature molecolare delle RSC, è stata eseguita l'analisi trascrittomica delle RSC stesse, della loro progenie PKHlow/neg e di colture primarie terminalmente differenziate e sono stati evidenziati geni differenzialmente espressi (DEG). L’analisi bioinfomatica mediante Gene Set Enrichment Analysis ha suggerito uno stato immaturo delle nostre RSC, ma comunque diverso da cellule staminali embrionali. Infine, incrociando le liste di DEG sono stati ottenuti potenziali marcatori e alcuni di essi sono stati selezionati e validati. In conclusione, abbiamo evidenziato le capacità differenziative in senso epiteliale sia prossimale che distale ed endoteliale delle cellule PKHhigh. Inoltre, abbiamo dimostrato che le cellule PKHhigh, completamente prive di qualsiasi marcatore endoteliale, sono in grado di dare origine a strutture simil-endoteliali. La coespressione occasionale di marcatori epiteliali ed endoteliali in strutture ripopolate potrebbe indicare uno stato precoce di transizione verso un differenziamento terminale. Il possibile ruolo della progenie PKHlow / neg nel velocizzare il differenziamento terminale delle cellule PKHhigh dovrà essere chiarito. Infine, la signature delle RSC ottenuta potrà aprire la possibilità di isolamento diretto di cellule staminali renali adulte da tessuto renale normale. The mechanism underlying the recovery of renal cell injury is still a matter of debate that concerns the involvement of fully differentiated cells, or the existence of quiescent scattered multipotent stem cells. By sphere forming assay and sorting, our group isolated a population of PKH26 most fluorescent cells with characteristics of adult renal stem-like cells (PKHhigh cells). We previously assessed the ability of PKHhigh cells to differentiate in vitro along epithelial, podocytic and endothelial lineages. We also demonstrated that PKHhigh population is heterogeneous in composition and within nephrospheres the cells with stem capacities are PKHhigh/CD133+/CD24- (RSC). We recently published that our nephrosphere cells, comprising PKHhigh cells and their PKHlow/neg progeny, are able to repopulate human decellularized renal scaffolds. With this research, we now aim: I) to prove the regenerative capabilities of PKHhigh stem-like cells, even in absence of their PKHlow/neg progeny. II) to find the molecular signature of RSC that among PKHhigh cells are those with the wider stem capacities. To reach these aims, we cultured isolated PKHhigh cells on acellular scaffolds for 30 days and the cells of the repopulated structures were characterized by sequential immunofluorescence using specific markers of differentiation. Some structures indicated a specific lineage differentiation into proximal and distal tubular epithelium and endothelium. Only few structures coexpressed some or all markers tested, indicating still immature phenotypes. For the disclosure of RSC molecular signature, transcriptomic analysis of RSC, of their PKHlow/neg progeny and of terminally differentiated primary cell cultures (PCC) was performed and differentially expressed genes (DEG) were evidenced. Bioinformatic Gene Set Enrichment Analysis suggested a renal immature status of our RSC, but different from embryonic stem cells. Crossing DEG lists potential markers were obtained and some of them were selected and validated. In conclusion, we highlighted the proximal and distal tubular epithelial and endothelial differentiative and regenerative abilities of PKHhigh cells. Moreover, we showed that PKHhigh cells, completely lacking any endothelial marker, were able to give rise to endothelial-like structures. The occasional coexpression of epithelial and endothelial markers in repopulated structures may indicate a transitional early status toward cell differentiation. The eventual role of the PKHlow/neg progeny of PKHhigh cells in speeding up the complete PKHhigh differentiation will be clarified. Finally, the obtained RSC signature would open the possibility for the direct isolation of adult renal stem-like cells from normal kidney tissue.

Published
2018

15. Correction: Arg tyrosine kinase modulates TGF-β1 production in human renal tubular cells under high-glucose conditions (doi:10.1242/jcs.183640)

Author

Torsello, Barbara, primary, Bianchi, Cristina, additional, Meregalli, Chiara, additional, Di Stefano, Vitalba, additional, Invernizzi, Lara, additional, De Marco, Sofia, additional, Bovo, Giorgio, additional, Brivio, Rinaldo, additional, Strada, Guido, additional, Bombelli, Silvia, additional, and Perego, Roberto A., additional

Published
2019
Full Text
View/download PDF

16. STUDY OF MULTIPOTENT RENAL PKHHIGH STEM-LIKE CELLS, ISOLATED FROM HUMAN NEPHROSPHERES: REGENERATIVE ABILITIES AND TRANSCRIPTOMIC PROFILE

Author

BOMBELLI, SILVIA, Meregalli, C, PEREGO, ROBERTO, MEREGALLI, CHIARA, BOMBELLI, SILVIA, Meregalli, C, PEREGO, ROBERTO, and MEREGALLI, CHIARA

Abstract

Il meccanismo alla base della riparazione di un danno a cellule renali è ancora oggi oggetto di dibattito e potrebbe coinvolgere sia cellule terminalmente differenziate che l'esistenza di cellule staminali multipotenti quiescenti. Sfruttando il saggio di formazione di sfere e la tecnica del FACS sorting, il nostro gruppo ha isolato una popolazione di cellule marcate col clorante PKH26 che avessero caratteristiche di cellule staminali renali adulte (PKHhigh). In precedenza abbiamo valutato la capacità di queste cellule di differenziare in vitro in lineage epiteliale, podocitico ed endoteliale. Abbiamo anche dimostrato che la popolazione PKHhigh è eterogenea nella sua composizione e all'interno delle nefrosfere le cellule con capacità simil-staminali sono PKHhigh/CD133+/CD24- (RSC). Abbiamo recentemente pubblicato che le nostre cellule delle nefrosfere, che comprendono cellule PKHhigh e la loro progenie PKHlow/neg, sono in grado di ripopolare scaffold renali umani decellularizzati. Con questa ricerca, ora abbiamo come scopo: i) di dimostrare le capacità rigenerative delle cellule staminali PKHhigh, anche in assenza della loro progenie PKHlow/neg. ii) di trovare la signature molecolare delle RSC che, tra PKHhigh, sono le cellule con capacità staminali più ampie. Per raggiungere questi obiettivi, abbiamo messo in coltura cellule PKHhigh su scaffold acellulari per 30 giorni e le cellule delle strutture ripopolate sono state caratterizzate mediante immunofluorescenza sequenziale, utilizzando specifici marcatori di differenziamento. Alcune strutture hanno indicato un differenziamento terminale in lineage epiteliale tubulare prossimale o distale o in quello endoteliale. Solo poche strutture mostravano invece la coespressione di alcuni o di tutti i marcatori testati, suggerendo un fenotipo ancora immaturo. Per la comprensione della signature molecolare delle RSC, è stata eseguita l'analisi trascrittomica delle RSC stesse, della loro progenie PKHlow/neg e di colture p, The mechanism underlying the recovery of renal cell injury is still a matter of debate that concerns the involvement of fully differentiated cells, or the existence of quiescent scattered multipotent stem cells. By sphere forming assay and sorting, our group isolated a population of PKH26 most fluorescent cells with characteristics of adult renal stem-like cells (PKHhigh cells). We previously assessed the ability of PKHhigh cells to differentiate in vitro along epithelial, podocytic and endothelial lineages. We also demonstrated that PKHhigh population is heterogeneous in composition and within nephrospheres the cells with stem capacities are PKHhigh/CD133+/CD24- (RSC). We recently published that our nephrosphere cells, comprising PKHhigh cells and their PKHlow/neg progeny, are able to repopulate human decellularized renal scaffolds. With this research, we now aim: I) to prove the regenerative capabilities of PKHhigh stem-like cells, even in absence of their PKHlow/neg progeny. II) to find the molecular signature of RSC that among PKHhigh cells are those with the wider stem capacities. To reach these aims, we cultured isolated PKHhigh cells on acellular scaffolds for 30 days and the cells of the repopulated structures were characterized by sequential immunofluorescence using specific markers of differentiation. Some structures indicated a specific lineage differentiation into proximal and distal tubular epithelium and endothelium. Only few structures coexpressed some or all markers tested, indicating still immature phenotypes. For the disclosure of RSC molecular signature, transcriptomic analysis of RSC, of their PKHlow/neg progeny and of terminally differentiated primary cell cultures (PCC) was performed and differentially expressed genes (DEG) were evidenced. Bioinformatic Gene Set Enrichment Analysis suggested a renal immature status of our RSC, but different from embryonic stem cells. Crossing DEG lists potential markers were obtained and some of them were selec

Published
2018

20. Grade-dependent lipid storage in ccRCC cells: molecular and functional study performed in primary cell cultures

Author

BIANCHI, CRISTINA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, SALERNO, FABIO ROSARIO, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Cifola, I, Mangano, E, Battaglia, C, Bovo, G, Viganò, P, Bianchi, C, Meregalli, C, Bombelli, S, Torsello, B, DE MARCO, S, Salerno, F, Cifola, I, Mangano, E, Battaglia, C, Bovo, G, Viganò, P, Strada, G, and Perego, R

Subjects
ccRCC , lipid storage, primary cell cultures
Published
2017

22. Nephrosphere-Derived Cells Are Induced to Multilineage Differentiation when Cultured on Human Decellularized Kidney Scaffolds

Author

Bombelli, Silvia, primary, Meregalli, Chiara, additional, Scalia, Carla, additional, Bovo, Giorgio, additional, Torsello, Barbara, additional, De Marco, Sofia, additional, Cadamuro, Massimiliano, additional, Viganò, Paolo, additional, Strada, Guido, additional, Cattoretti, Giorgio, additional, Bianchi, Cristina, additional, and Perego, Roberto A., additional

Published
2018
Full Text
View/download PDF

23. Evaluation of the capability of PKHhigh human adult renal stem-like cells to repopulate human decellularized renal scaffolds

Author

Meregalli, C, Bombelli, S, Bovo, G, Torsello, B, DE MARCO, S, Viganò, P, Cattoretti, G, Bianchi, C, Perego, R, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, CATTORETTI, GIORGIO, BIANCHI, CRISTINA, PEREGO, ROBERTO, Meregalli, C, Bombelli, S, Bovo, G, Torsello, B, DE MARCO, S, Viganò, P, Cattoretti, G, Bianchi, C, Perego, R, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, CATTORETTI, GIORGIO, BIANCHI, CRISTINA, and PEREGO, ROBERTO

Published
2017

24. Grade-dependent lipid storage in ccRCC cells: molecular and functional study performed in primary cell cultures

Author

Bianchi, C, Meregalli, C, Bombelli, S, Torsello, B, DE MARCO, S, Salerno, F, Cifola, I, Mangano, E, Battaglia, C, Bovo, G, Viganò, P, Strada, G, Perego, R, BIANCHI, CRISTINA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, SALERNO, FABIO ROSARIO, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Bianchi, C, Meregalli, C, Bombelli, S, Torsello, B, DE MARCO, S, Salerno, F, Cifola, I, Mangano, E, Battaglia, C, Bovo, G, Viganò, P, Strada, G, Perego, R, BIANCHI, CRISTINA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, SALERNO, FABIO ROSARIO, STRADA, GUIDO RAFFAELE, and PEREGO, ROBERTO

Published
2017

25. Role of Lysyl oxidase, TGFb1 and Arg tyrosine kinase in invasive and metastatic properties of ccRCC cells

Author

Bianchi, C, DE MARCO, S, Torsello, B, DI STEFANO, V, Meregalli, C, Bombelli, S, Bovo, G, Viganò, P, Strada, G, Perego, R, BIANCHI, CRISTINA, DE MARCO, SOFIA, TORSELLO, BARBARA ROSA, DI STEFANO, VITALBA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Bianchi, C, DE MARCO, S, Torsello, B, DI STEFANO, V, Meregalli, C, Bombelli, S, Bovo, G, Viganò, P, Strada, G, Perego, R, BIANCHI, CRISTINA, DE MARCO, SOFIA, TORSELLO, BARBARA ROSA, DI STEFANO, VITALBA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, and PEREGO, ROBERTO

Published
2017

26. Repopulation of human decellularized renal scaffolds by renal stem-like cells and progenitors within nephrospheres

Author

Meregalli, C, Bombelli, S, Bovo, G, Torsello, B, DE MARCO, S, Viganò, P, Cattoretti, G, Bianchi, C, Perego, R, Meregalli Chiara, Bombelli Silvia, Bovo Giorgio, Torsello Barbara, De marco Sofia, Viganò Paolo, Cattoretti Giorgio, Bianchi Cristina, Perego roberto, Meregalli, C, Bombelli, S, Bovo, G, Torsello, B, DE MARCO, S, Viganò, P, Cattoretti, G, Bianchi, C, Perego, R, Meregalli Chiara, Bombelli Silvia, Bovo Giorgio, Torsello Barbara, De marco Sofia, Viganò Paolo, Cattoretti Giorgio, Bianchi Cristina, and Perego roberto

Published
2017

27. Endogenous lysysl oxidase in tumor progression and collagen stiffness: study performed in human clear cell Renal Cell Carcinoma primary cell cultures

Author

TORSELLO, BARBARA ROSA, BIANCHI, CRISTINA, CASSINA, VALERIA, DE MARCO, SOFIA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Bovo, G, Viganò, P, Torsello, B, Bianchi, C, Bovo, G, Cassina, V, DE MARCO, S, Meregalli, C, Bombelli, S, Viganò, P, Strada, G, and Perego, R

Subjects
ccRCC, lox, primary cell culture
Published
2016

28. Endogenous lysyl-oxidase in clear cell renal cell carcinoma promotes tumor progression and collagen stiffness

Author

TORSELLO, BARBARA ROSA, BIANCHI, CRISTINA, CASSINA, VALERIA, CORTI, ROBERTA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Di Stefano, V, Battaglia, C, Cifola, I, Mangano, E, Bovo, G, De Marco, S, Vigano’, P, Torsello, B, Di Stefano, V, Bianchi, C, Battaglia, C, Cifola, I, Mangano, E, Bovo, G, Cassina, V, De Marco, S, Corti, R, Meregalli, C, Bombelli, S, Vigano’, P, Strada, G, and Perego, R

Subjects
ccRCC, lox
Published
2016

29. Transcriptomic profiling of multipotent renal nephrosphere PKHhigh cells for the identification of a renal stem signature

Author

MEREGALLI, CHIARA, BOMBELLI, SILVIA, RANZANI, VALERIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, CATTORETTI, GIORGIO, STRADA, GUIDO RAFFAELE, BIANCHI, CRISTINA, PEREGO, ROBERTO, Rossetti, G, Bovo, G, Pagani, M, Meregalli, C, Bombelli, S, Rossetti, G, Ranzani, V, Torsello, B, DE MARCO, S, Bovo, G, Pagani, M, Cattoretti, G, Strada, G, Bianchi, C, and Perego, R

Subjects
multipotent renal cells, Transcriptomic profiling, renal stem signature, nephrosphere, MED/04 - PATOLOGIA GENERALE
Published
2016

30. Primary cell cultures obtained from clear cell renal cell carcinoma specimens retain the transcriptomic profile and metabolic phenotype of corresponding tissues

Author

BIANCHI, CRISTINA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, MARCO S, D, Cifola, I, Mangano, E, Battaglia, C, Bovo, G, Vigano’, P, Bianchi, C, Meregalli, C, Bombelli, S, Torsello, B, MARCO S, D, Cifola, I, Mangano, E, Battaglia, C, Bovo, G, Vigano’, P, Strada, G, and Perego, R

Subjects
ccRCC, trascriptomic profile, metabolism, primary cell culture
Published
2016

31. Transcriptomic profile of PKHhigh multipotent renal cells for the identification of a renal stem cell signature

Author

MEREGALLI, CHIARA, BOMBELLI, SILVIA, RANZANI, VALERIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, PAGANI, MARCO, CATTORETTI, GIORGIO, STRADA, GUIDO RAFFAELE, BIANCHI, CRISTINA, PEREGO, ROBERTO, Rossetti, G, Bovo, G, Meregalli, C, Bombelli, S, Rossetti, G, Ranzani, V, Torsello, B, DE MARCO, S, Bovo, G, Pagani, M, Cattoretti, G, Strada, G, Bianchi, C, and Perego, R

Subjects
renal adult stem cell, trascriptomic profile
Published
2016

32. Annexin A4 involvement in lipid storage of clear cell Renal Cell Carcinoma

Author

BIANCHI, CRISTINA, BOMBELLI, SILVIA, MEREGALLI, CHIARA, TORSELLO, BARBARA ROSA, PEREGO, ROBERTO, Bovo, G, Viganò, P, Strada, G, Bianchi, C, Bombelli, S, Meregalli, C, Torsello, B, Bovo, G, Viganò, P, Strada, G, and Perego, R

Subjects
Annexin A4, lipid storage, ccRCC
Published
2015

34. Endogenous lysyl-oxidase in clear cell renal cell carcinoma promotes tumor progression and collagen stiffness

Author

Torsello, B, Di Stefano, V, Bianchi, C, Battaglia, C, Cifola, I, Mangano, E, Bovo, G, Cassina, V, De Marco, S, Corti, R, Meregalli, C, Bombelli, S, Vigano’, P, Strada, G, Perego, R, TORSELLO, BARBARA ROSA, BIANCHI, CRISTINA, CASSINA, VALERIA, CORTI, ROBERTA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Torsello, B, Di Stefano, V, Bianchi, C, Battaglia, C, Cifola, I, Mangano, E, Bovo, G, Cassina, V, De Marco, S, Corti, R, Meregalli, C, Bombelli, S, Vigano’, P, Strada, G, Perego, R, TORSELLO, BARBARA ROSA, BIANCHI, CRISTINA, CASSINA, VALERIA, CORTI, ROBERTA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, and PEREGO, ROBERTO

Published
2016

35. TGF-b1 production is modulated by Arg tyrosine kinase in high glucose treated human renal tubular cells

Author

Bianchi, C, Torsello, B, Meregalli, C, DE MARCO, S, Bovo, G, Brivio, R, Strada, G, Bombelli, S, Perego, R, BIANCHI, CRISTINA, TORSELLO, BARBARA ROSA, MEREGALLI, CHIARA, DE MARCO, SOFIA, BRIVIO, RINALDO, STRADA, GUIDO RAFFAELE, BOMBELLI, SILVIA, PEREGO, ROBERTO, Bianchi, C, Torsello, B, Meregalli, C, DE MARCO, S, Bovo, G, Brivio, R, Strada, G, Bombelli, S, Perego, R, BIANCHI, CRISTINA, TORSELLO, BARBARA ROSA, MEREGALLI, CHIARA, DE MARCO, SOFIA, BRIVIO, RINALDO, STRADA, GUIDO RAFFAELE, BOMBELLI, SILVIA, and PEREGO, ROBERTO

Published
2016

36. Endogenous lysysl oxidase in tumor progression and collagen stiffness: study performed in human clear cell Renal Cell Carcinoma primary cell cultures

Author

Torsello, B, Bianchi, C, Bovo, G, Cassina, V, DE MARCO, S, Meregalli, C, Bombelli, S, Viganò, P, Strada, G, Perego, R, TORSELLO, BARBARA ROSA, BIANCHI, CRISTINA, CASSINA, VALERIA, DE MARCO, SOFIA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, PEREGO, ROBERTO, Torsello, B, Bianchi, C, Bovo, G, Cassina, V, DE MARCO, S, Meregalli, C, Bombelli, S, Viganò, P, Strada, G, Perego, R, TORSELLO, BARBARA ROSA, BIANCHI, CRISTINA, CASSINA, VALERIA, DE MARCO, SOFIA, MEREGALLI, CHIARA, BOMBELLI, SILVIA, STRADA, GUIDO RAFFAELE, and PEREGO, ROBERTO

Published
2016

37. Transcriptomic profiling of multipotent renal nephrosphere PKHhigh cells for the identification of a renal stem signature

Author

Meregalli, C, Bombelli, S, Rossetti, G, Ranzani, V, Torsello, B, DE MARCO, S, Bovo, G, Pagani, M, Cattoretti, G, Strada, G, Bianchi, C, Perego, R, MEREGALLI, CHIARA, BOMBELLI, SILVIA, RANZANI, VALERIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, CATTORETTI, GIORGIO, STRADA, GUIDO RAFFAELE, BIANCHI, CRISTINA, PEREGO, ROBERTO, Meregalli, C, Bombelli, S, Rossetti, G, Ranzani, V, Torsello, B, DE MARCO, S, Bovo, G, Pagani, M, Cattoretti, G, Strada, G, Bianchi, C, Perego, R, MEREGALLI, CHIARA, BOMBELLI, SILVIA, RANZANI, VALERIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, CATTORETTI, GIORGIO, STRADA, GUIDO RAFFAELE, BIANCHI, CRISTINA, and PEREGO, ROBERTO

Published
2016

38. Transcriptomic profile of PKHhigh multipotent renal cells for the identification of a renal stem cell signature

Author

Meregalli, C, Bombelli, S, Rossetti, G, Ranzani, V, Torsello, B, DE MARCO, S, Bovo, G, Pagani, M, Cattoretti, G, Strada, G, Bianchi, C, Perego, R, MEREGALLI, CHIARA, BOMBELLI, SILVIA, RANZANI, VALERIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, PAGANI, MARCO, CATTORETTI, GIORGIO, STRADA, GUIDO RAFFAELE, BIANCHI, CRISTINA, PEREGO, ROBERTO, Meregalli, C, Bombelli, S, Rossetti, G, Ranzani, V, Torsello, B, DE MARCO, S, Bovo, G, Pagani, M, Cattoretti, G, Strada, G, Bianchi, C, Perego, R, MEREGALLI, CHIARA, BOMBELLI, SILVIA, RANZANI, VALERIA, TORSELLO, BARBARA ROSA, DE MARCO, SOFIA, PAGANI, MARCO, CATTORETTI, GIORGIO, STRADA, GUIDO RAFFAELE, BIANCHI, CRISTINA, and PEREGO, ROBERTO

Published
2016

40. Nephrosphere-derived cells cultured on decellularized kidney scaffolds can differentiate into multiple lineages

Author

Bombelli, S, Scalia, C, Meregalli, C, Torsello, B, Bovo, G, Cattoretti, G, Viganò, P, Strada, G, Bianchi, C, Perego, R, BOMBELLI, SILVIA, MEREGALLI, CHIARA, TORSELLO, BARBARA ROSA, CATTORETTI, GIORGIO, BIANCHI, CRISTINA, PEREGO, ROBERTO, Bombelli, S, Scalia, C, Meregalli, C, Torsello, B, Bovo, G, Cattoretti, G, Viganò, P, Strada, G, Bianchi, C, Perego, R, BOMBELLI, SILVIA, MEREGALLI, CHIARA, TORSELLO, BARBARA ROSA, CATTORETTI, GIORGIO, BIANCHI, CRISTINA, and PEREGO, ROBERTO

Published
2015

41. Modulazione del metabolismo lipidico e glicidico in colture cellulari primarie e cancer stem cells in ccRCC: un nuovo approccio per l’identificazione di target terapeutici

Author

Meregalli, C, Bombelli, S, Torsello, B, Bovo, G, Viganò, P, Perego, R, Bianchi, C, Strada, G, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, PEREGO, ROBERTO, BIANCHI, CRISTINA, Strada, G., Meregalli, C, Bombelli, S, Torsello, B, Bovo, G, Viganò, P, Perego, R, Bianchi, C, Strada, G, MEREGALLI, CHIARA, BOMBELLI, SILVIA, TORSELLO, BARBARA ROSA, PEREGO, ROBERTO, BIANCHI, CRISTINA, and Strada, G.

Published
2015

42. Annexin A4 involvement in lipid storage of clear cell Renal Cell Carcinoma

Author

Bianchi, C, Bombelli, S, Meregalli, C, Torsello, B, Bovo, G, Viganò, P, Strada, G, Perego, R, BIANCHI, CRISTINA, BOMBELLI, SILVIA, MEREGALLI, CHIARA, TORSELLO, BARBARA ROSA, PEREGO, ROBERTO, Bianchi, C, Bombelli, S, Meregalli, C, Torsello, B, Bovo, G, Viganò, P, Strada, G, Perego, R, BIANCHI, CRISTINA, BOMBELLI, SILVIA, MEREGALLI, CHIARA, TORSELLO, BARBARA ROSA, and PEREGO, ROBERTO

Published
2015

43. PKH high /CD133+/CD24- Renal Stem-Like Cells Isolated from Human Nephrospheres Exhibit In Vitro Multipotency.

Author

Bombelli S, Meregalli C, Grasselli C, Bolognesi MM, Bruno A, Eriani S, Torsello B, Marco S, Bernasconi DP, Zucchini N, Mazzola P, Bianchi C, Grasso M, Albini A, Cattoretti G, and Perego RA

Subjects
Adult, Aged, Aged, 80 and over, Biocompatible Materials metabolism, Cell Differentiation physiology, Cells, Cultured, Collagen metabolism, Drug Combinations, Female, Humans, Laminin metabolism, Male, Middle Aged, Proteoglycans metabolism, AC133 Antigen metabolism, CD24 Antigen metabolism, Fluorescent Dyes metabolism, Kidney cytology, Multipotent Stem Cells metabolism, Organic Chemicals metabolism
Abstract

The mechanism upon which human kidneys undergo regeneration is debated, though different lineage-tracing mouse models have tried to explain the cellular types and the mechanisms involved. Different sources of human renal progenitors have been proposed, but it is difficult to argue whether these populations have the same capacities that have been described in mice. Using the nephrosphere (NS) model, we isolated the quiescent population of adult human renal stem-like PKH high /CD133+/CD24- cells (RSC). The aim of this study was to deepen the RSC in vitro multipotency capacity. RSC, not expressing endothelial markers, generated secondary nephrospheres containing CD31+/vWf+ cells and cytokeratin positive cells, indicating the coexistence of endothelial and epithelial commitment. RSC cultured on decellularized human renal scaffolds generated endothelial structures together with the proximal and distal tubular structures. CD31+ endothelial committed progenitors sorted from nephrospheres generated spheroids with endothelial-like sprouts in Matrigel. We also demonstrated the double commitment toward endothelial and epithelial lineages of single RSC. The ability of the plastic RSC population to recapitulate the development of tubular epithelial and endothelial renal lineages makes these cells a good tool for the creation of organoids with translational relevance for studying the parenchymal and endothelial cell interactions and developing new therapeutic strategies.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results