Search

Your search keyword '"Mertens, Pascal"' showing total 225 results
Start Over Author "Mertens, Pascal"
225 results on '"Mertens, Pascal"'

7. Development of an immunochromatographic lateral flow assay to rapidly detect OXA-23-, OXA-40-, OXA-58- and NDM-mediated carbapenem resistance determinants in Acinetobacter baumannii

Author

Mertins, Sonja, primary, Higgins, Paul G., additional, Thunissen, Caroline, additional, Magein, Henri, additional, Gilleman, Quentin, additional, Mertens, Pascal, additional, Rodríguez, María González, additional, Maus, Liza Marie, additional, Seifert, Harald, additional, Krönke, Martin, additional, and Klimka, Alexander, additional

Published
2023
Full Text
View/download PDF

16. Emergent canine visceral leishmaniasis in Argentina: Comparative diagnostics and relevance to proliferation of human disease

Author

Fujisawa, Kyoko, primary, Silcott-Niles, Charlotte, additional, Simonson, Poppy, additional, Lamattina, Daniela, additional, Humeres, Cristian A., additional, Bhattacharyya, Tapan, additional, Mertens, Pascal, additional, Thunissen, Caroline, additional, O’Rourke, Victoria, additional, Pańczuk, Magdalena, additional, Whitworth, James A., additional, Salomón, Oscar Daniel, additional, and Miles, Michael A., additional

Published
2021
Full Text
View/download PDF

17. Additional file 3 of Assessing antibody decline after chemotherapy of early chronic Chagas disease patients

Author

Murphy, Niamh, Cardinal, M. Victoria, Bhattacharyya, Tapan, Enriquez, Gustavo F., Macchiaverna, Natalia P., Alvedro, Alejandra, Freilij, Héctor, Martinez de Salazar, Pablo, Molina, Israel, Mertens, Pascal, Gilleman, Quentin, Gürtler, Ricardo E., and Miles, Michael A.

Abstract

Additional file 3: Table S1. Multiple logistic regression analysis of antibody decline as a function of selected predictors.

Published
2021
Full Text
View/download PDF

18. Additional file 4 of Assessing antibody decline after chemotherapy of early chronic Chagas disease patients

Author

Murphy, Niamh, Cardinal, M. Victoria, Bhattacharyya, Tapan, Enriquez, Gustavo F., Macchiaverna, Natalia P., Alvedro, Alejandra, Freilij, Héctor, Martinez de Salazar, Pablo, Molina, Israel, Mertens, Pascal, Gilleman, Quentin, Gürtler, Ricardo E., and Miles, Michael A.

Abstract

Additional file 4: Table S2. Multiple logistic regression analysis of seronegativisation as a function of selected predictors.

Published
2021
Full Text
View/download PDF

20. Development and Potential Usefulness of the COVID-19 Ag Respi-Strip Diagnostic Assay in a Pandemic Context

Author

Mertens, Pascal, De Vos, Nathalie, Martiny, Delphine, Jassoy, Christian, Mirazimi, Ali, Cuypers, Lize, Van den Wijngaert, Sigi, Monteil, Vanessa, Melin, Pierrette, Stoffels, Karolien, Yin, Nicolas, Mileto, Davide, Delaunoy, Sabrina, Magein, Henri, Lagrou, Katrien, Bouzet, Justine, Serrano, Gabriela, Wautier, Magali, Leclipteux, Thierry, Van Ranst, Marc, Vandenberg, Olivier, working diagnostic group, LHUB-ULB SARS-CoV-2, Mertens, Pascal, De Vos, Nathalie, Martiny, Delphine, Jassoy, Christian, Mirazimi, Ali, Cuypers, Lize, Van den Wijngaert, Sigi, Monteil, Vanessa, Melin, Pierrette, Stoffels, Karolien, Yin, Nicolas, Mileto, Davide, Delaunoy, Sabrina, Magein, Henri, Lagrou, Katrien, Bouzet, Justine, Serrano, Gabriela, Wautier, Magali, Leclipteux, Thierry, Van Ranst, Marc, Vandenberg, Olivier, and working diagnostic group, LHUB-ULB SARS-CoV-2

Abstract

Introduction: COVID-19 Ag Respi-Strip, an immunochromatographic (ICT) assay for the rapid detection of SARS-CoV-2 antigen on nasopharyngeal specimen, has been developed to identify positive COVID-19 patients allowing prompt clinical and quarantine decisions. In this original research article, we describe the conception, the analytical and clinical performances as well as the risk management of implementing the COVID-19 Ag Respi-Strip in a diagnostic decision algorithm. Materials and Methods: Development of the COVID-19 Ag Respi-Strip resulted in a ready-to-use ICT assay based on a membrane technology with colloidal gold nanoparticles using monoclonal antibodies directed against the SARS-CoV and SARS-CoV-2 highly conserved nucleoprotein antigen. Four hundred observations were recorded for the analytical performance study and thirty tests were analyzed for the crossreactivity study. The clinical performance study was performed in a retrospective multicentric evaluation on aliquots of 328 nasopharyngeal samples. COVID-19 Ag Respi-Strip results were compared with qRT-PCR as golden standard for COVID-19 diagnostics. Results: In the analytical performance study, the reproducibility showed a between-observer disagreement of 1.7%, a robustness of 98%, an overall satisfying user friendliness and no cross-reactivity with other virus-infected nasopharyngeal samples. In the clinical performance study performed in three different clinical laboratories during the ascendant phase of the epidemiological curve, we found an overall sensitivity and specificity of 57.6 and 99.5%, respectively with an accuracy of 82.6%. The cut-off of the ICT was found at CT < 22. User-friendliness analysis and risk management assessment through Ishikawa diagram demonstrate that COVID-19 Ag Respi-Strip may be implemented in clinical laboratories according to biosafety recommendations. Conclusion: The COVID-19 Ag Respi-Strip represents a promising rapid SARS-CoV-2 antigen assay for the first-line diagnos

Published
2020

23. Diagnostic accuracy of direct agglutination test, rK39 ELISA and six rapid diagnostic tests among visceral leishmaniasis patients with and without HIV coinfection in Ethiopia

Author

Kassa, Mekibib, primary, Abdellati, Saïd, additional, Cnops, Lieselotte, additional, Bremer Hinckel, Bruno C., additional, Yeshanew, Arega, additional, Hailemichael, Wasihun, additional, Vogt, Florian, additional, Adriaensen, Wim, additional, Mertens, Pascal, additional, Diro, Ermias, additional, van Griensven, Johan, additional, and Van den Bossche, Dorien, additional

Published
2020
Full Text
View/download PDF

24. Correction: A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

Author

McClean, Mairi C. W., primary, Bhattacharyya, Tapan, additional, Mertens, Pascal, additional, Murphy, Niamh, additional, Gilleman, Quentin, additional, Gustin, Yves, additional, Zeippen, Nicolas, additional, Xavier, Samanta C. C., additional, Jansen, Ana M., additional, and Miles, Michael A., additional

Published
2020
Full Text
View/download PDF

25. A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

Author

McClean, Mairi C. W., primary, Bhattacharyya, Tapan, additional, Mertens, Pascal, additional, Murphy, Niamh, additional, Gilleman, Quentin, additional, Gustin, Yves, additional, Zeippen, Nicolas, additional, Xavier, Samanta C. C., additional, Jansen, Ana M., additional, and Miles, Michael A., additional

Published
2020
Full Text
View/download PDF

27. Field study of the improved rapid sand fly exposure test in areas endemic for canine leishmaniasis

Author

Willen, Laura, Lestinova, Tereza, Kalousková, Barbora, Sumova, Petra, Spitzova, Tatiana, Velez, Rita, Domenech, Ester, Vaněk, Ondřej, Gállego, Montserrat, Mertens, Pascal, Volf, Petr, and Universitat de Barcelona

Subjects
Physiology, Protein Expression, RC955-962, Patologia veterinària, Disease Vectors, Zoonoses, Arctic medicine. Tropical medicine, Medicine and Health Sciences, Dog Diseases, Enzyme-Linked Immunoassays, Leishmaniasis, Mammals, Protozoans, Leishmania, Immunoassay, Mice, Inbred BALB C, Eukaryota, Body Fluids, Infectious Diseases, Blood, Vertebrates, Female, Anatomy, Public aspects of medicine, RA1-1270, Research Article, Neglected Tropical Diseases, Arthropoda, Leishmania Infantum, Research and Analysis Methods, Veterinary pathology, Dogs, Leishmaniosi, parasitic diseases, Parasitic Diseases, Gene Expression and Vector Techniques, Animals, Immunoassays, Molecular Biology Techniques, Molecular Biology, Molecular Biology Assays and Analysis Techniques, Protozoan Infections, Organisms, Biology and Life Sciences, Tropical Diseases, Invertebrates, Parasitic Protozoans, Insect Vectors, Sand Flies, Species Interactions, Phlebotomus, Amniotes, Immunologic Techniques
Abstract

Background Canine leishmaniasis (CanL) is a severe chronic disease caused by Leishmania infantum and transmitted by sand flies of which the main vector in the Western part of the Mediterranean basin is Phlebotomus perniciosus. Previously, an immunochromatographic test (ICT) was proposed to allow rapid evaluation of dog exposure to P. perniciosus. In the present study, we optimized the prototype and evaluated the detection accuracy of the ICT in field conditions. Possible cross-reactions with other hematophagous arthropods were also assessed. Methodology/Principal findings The ICT was optimized by expressing the rSP03B protein in a HEK293 cell line, which delivered an increased specificity (94.92%). The ICT showed an excellent reproducibility and inter-person reliability, and was optimized for use with whole canine blood which rendered an excellent degree of agreement with the use of serum. Field detectability of the ICT was assessed by screening 186 dogs from different CanL endemic areas with both the SGH-ELISA and the ICT, and 154 longitudinally sampled dogs only with the ICT. The ICT results corresponded to the SGH-ELISA for most areas, depending on the statistical measure used. Furthermore, the ICT was able to show a clear seasonal fluctuation in the proportion of bitten dogs. Finally, we excluded cross-reactions between non-vector species and confirmed favorable cross-reactions with other L. infantum vectors belonging to the subgenus Larroussius. Conclusions/Significance We have successfully optimized the ICT, now also suitable to be used with whole canine blood. The test is able to reflect the seasonal fluctuation in dog exposure and showed a good detectability in a field population of naturally exposed dogs, particularly in areas with a high seroprevalence of bitten dogs. Furthermore, our study showed the existence of favorable cross-reactions with other sand fly vectors thereby expanding its use in the field., Author summary Canine leishmaniasis (CanL) is a widespread severe chronic disease in dogs, caused by the protozoan parasite Leishmania infantum. Leishmania parasites are transmitted by the bite of phlebotomine sand flies, of which the most important one in southwestern Europe is Phlebotomus perniciosus. A sand fly bite is accompanied by the inoculation of saliva, which elicits a specific antibody response in the host. Past studies have used these antibodies as markers of exposure to sand flies. Recently, a rapid sand fly exposure test was prepared using one salivary protein of P. perniciosus, the SP03B protein. This rapid test possessed a high sensitivity and specificity when screening experimentally exposed versus non-bitten dogs. However, since naturally exposed dogs possess a higher variability of antibodies against sand fly saliva, we have optimized the previously proposed rapid test and evaluated its detectability on field samples. In this study we present the optimized rSP03B sero-strip that possesses a higher specificity and an ability to detect seropositive dogs from a naturally exposed dog population in a similar fashion as standard serological methods. Furthermore, we have optimized it with whole canine blood which renders it suitable for use in field conditions.

Published
2019

28. MOESM1 of Lineage-specific rapid diagnostic tests can resolve Trypanosoma cruzi TcII/V/VI ecological and epidemiological associations in the Argentine Chaco

Author

Murphy, Niamh, Macchiaverna, Natalia, M. Victoria Cardinal, Bhattacharyya, Tapan, Mertens, Pascal, Zeippen, Nicolas, Gustin, Yves, Gilleman, Quentin, GĂźrtler, Ricardo, and Miles, Michael

Subjects
parasitic diseases
Abstract

Additional file 1: Table S1. Trypanosoma cruzi lineage-specific peptides (TSSApep), with polymorphisms underlined. Table S2. Available corresponding T. cruzi genotyping information from humans and animals tested by TSSA lineage-specific serology.

Published
2019
Full Text
View/download PDF

29. Prevalence of Giardia intestinalis infection in schistosomiasis-endemic areas in South-Central Mali

Author

Fofana, Hassan K.M., Schwarzkopf, Maren, Doumbia, Mama N., Saye, Rénion, Nimmesgern, Anna, Landouré, Aly, Traoré, Mamadou S., Mertens, Pascal, Utzinger, Jürg, Sacko, Moussa, and Becker, Sören L.

Subjects
polymerase chain reaction, lcsh:R, diarrhea, lcsh:Medicine, Schistosoma mansoni, Giardia intestinalis, stool microscopy, Mali, rapid diagnostic test, Article, BD Max Enteric Parasite Panel, parasitic diseases
Abstract

Intestinal parasite infections are frequent causes of diarrhea and malnutrition among children in the tropics. Transmission of helminths and intestinal protozoa is intimately connected with conditions of poverty, including inadequate sanitation and hygiene. Concurrent infections with several intestinal pathogens may lead to excess morbidity. Yet, there is a paucity of epidemiological data from Mali. In this study, stool samples from 56 individuals, aged 2&ndash, 63 years, from Bamako and Niono, south-central Mali were examined for intestinal parasites using stool microscopy. Additionally, stool samples were subjected to a rapid diagnostic test (RDT) and polymerase chain reaction (PCR) for the detection of Cryptosporidium spp. and Giardia intestinalis. The predominant pathogens were Schistosoma mansoni and G. intestinalis with prevalences of 41% and 38%, respectively. Hymenolepis nana was detected in 4% of the participants, while no eggs of soil-transmitted helminths were found. Concurrent infections with G. intestinalis and S. mansoni were diagnosed in 16% of the participants. For the detection of G. intestinalis, PCR was more sensitive (100%) than RDT (62%) and microscopy (48%). As helminth-protozoa coinfections might have important implications for morbidity control programs, future studies should employ diagnostic tools beyond stool microscopy to accurately assess the co-endemicity of giardiasis and schistosomiasis.

Published
2019
Full Text
View/download PDF

33. Generation and selection of antibodies for a novel immunochromatographic lateral flow test to rapidly identify OXA-23-like-mediated carbapenem resistance in Acinetobacter baumannii

Author

Mertins, Sonja, Higgins, Paul G., Rodriguez, Maria Gonzalez, Borlon, Celine, Galleman, Quentin, Mertens, Pascal, Seifert, Harald, Kroenke, Martin, Klimka, Alexander, Mertins, Sonja, Higgins, Paul G., Rodriguez, Maria Gonzalez, Borlon, Celine, Galleman, Quentin, Mertens, Pascal, Seifert, Harald, Kroenke, Martin, and Klimka, Alexander

Abstract

Introduction. The spread of carbapenem-resistant Acinetobacter baumannii has led to a worldwide healthcare problem. Carbapenem resistance in A. baumannii is mainly mediated by the acquisition of the carbapenem-hydrolyzing oxacillinase OXA-23. The phenotypic detection of carbapenem-producing A. baumannii is challenging and time-consuming. Hence, there is an unmet medical need for reliable and rapid diagnostic tools to detect OXA-23-producing Acinetobacter isolates to enable successful patient management. Aim. Development of an immunochromatographic lateral flow test (ICT) for the rapid and reliable detection of OXA-23-producing carbapenem-resistant Acinetobacter isolates. Methodology. For the development of an antibody-based ICT, we generated anti-OXA-23 monoclonal antibodies (MoAbs) and screened them sequentially for their ability to bind native OXA-23. Selected OXA-23-specific MoAbs were tested in different combinations for their capacity to capture and detect OXA-23(His6) by sandwich enzyme-linked immunosorbent assay (ELISA) and ICT. A well-characterized collection of carbapenem-resistant Acinetobacter isolates with defined carbapenem resistance mechanisms were used to evaluate the specificity of the final OXA-23 ICT prototype. Results. The antibody pairs best suited for the sandwich ELISA format did not match the best pairs in the ICT format selected during the development process of the final prototype OXA-23 ICT. This prototype was able to differentiate between OXA-23 subfamily-mediated carbapenem resistance and carbapenem-resistant Acinetobacter isolates overexpressing other OXAs with 100 % specificity and a turnaround time of 20 min from culture plate to result. Conclusion. With this rapid detection assay one can save 12-48 h of diagnostic time, which could help avoid inappropriate use of carbapenems and enable earlier intervention to control the transmission of OXA-23-producing carbapenem-resistant Acinetobacter isolates to other patients and healthcare work

Published
2019

35. Generation and selection of antibodies for a novel immunochromatographic lateral flow test to rapidly identify OXA-23-like-mediated carbapenem resistance in Acinetobacter baumannii

Author

Mertins, Sonja, primary, Higgins, Paul G., additional, Rodríguez, María González, additional, Borlon, Céline, additional, Gilleman, Quentin, additional, Mertens, Pascal, additional, Seifert, Harald, additional, Krönke, Martin, additional, and Klimka, Alexander, additional

Published
2019
Full Text
View/download PDF

36. Refining wet lab experiments with in silico searches: A rational quest for diagnostic peptides in visceral leishmaniasis

Author

Bremer Hinckel, Bruno Cesar, primary, Marlais, Tegwen, additional, Airs, Stephanie, additional, Bhattacharyya, Tapan, additional, Imamura, Hideo, additional, Dujardin, Jean-Claude, additional, El-Safi, Sayda, additional, Singh, Om Prakash, additional, Sundar, Shyam, additional, Falconar, Andrew Keith, additional, Andersson, Bjorn, additional, Litvinov, Sergey, additional, Miles, Michael A., additional, and Mertens, Pascal, additional

Published
2019
Full Text
View/download PDF

37. Molecular and culture-based diagnosis of Clostridium difficile isolates from Côte d'Ivoire after prolonged storage at disrupted cold chain conditions

Author

Becker, Sören L., Chatigre, Justin K., Coulibaly, Jean T., Mertens, Pascal, Bonfoh, Bassirou, Herrmann, Mathias, Kuijper, Ed J., N'Goran, Eliézer K., Utzinger, Jürg, and von Müller, Lutz

Abstract

Background Although Clostridium difficile is a major cause of diarrhoea, its epidemiology in tropical settings is poorly understood. Strain characterisation requires work-up in specialised laboratories, often after prolonged storage without properly maintained cold chain. Methods We screened 298 human faecal samples from Côte d'Ivoire using a rapid test for C. difficile glutamate dehydrogenase (GDH). GDH-positive samples were aerobically stored at disrupted cold chain conditions (mean duration: 11 days) before transfer to a reference laboratory for anaerobic culture, susceptibility testing, PCR assays and ribotyping. Results Sixteen samples (5.4%) had a positive GDH screening test. C. difficile infection was confirmed in six specimens by culture and PCR, while no nucleic acids of C. difficile were detected in the culture-negative samples. Further analysis of stool samples harbouring toxigenic C. difficile strains confirmed that both GDH and toxins remained detectable for at least 28 days, regardless of storage conditions (aerobic storage at 4°C or 20°C). Conclusions Storage conditions only minimally affect recovery of C. difficile and its toxins in stool culture. A rapid GDH screening test and subsequent transfer of GDH-positive stool samples to reference laboratories for in-depth characterisation may improve our understanding of the epidemiology of C. difficile in the tropics

Published
2017

39. Visceral Leishmaniasis IgG1 Rapid Monitoring of Cure vs. Relapse, and Potential for Diagnosis of Post Kala-Azar Dermal Leishmaniasis

Author

Marlais, Tegwen, primary, Bhattacharyya, Tapan, additional, Singh, Om Prakash, additional, Mertens, Pascal, additional, Gilleman, Quentin, additional, Thunissen, Caroline, additional, Hinckel, Bruno C. Bremer, additional, Pearson, Callum, additional, Gardner, Bathsheba L., additional, Airs, Stephanie, additional, de la Roche, Marianne, additional, Hayes, Kiera, additional, Hafezi, Hannah, additional, Falconar, Andrew K., additional, Eisa, Osama, additional, Saad, Alfarazdeg, additional, Khanal, Basudha, additional, Bhattarai, Narayan Raj, additional, Rijal, Suman, additional, Boelaert, Marleen, additional, El-Safi, Sayda, additional, Sundar, Shyam, additional, and Miles, Michael A., additional

Published
2018
Full Text
View/download PDF

43. Detection of Immunoglobulin G1 Against rK39 Improves Monitoring of Treatment Outcomes in Visceral Leishmaniasis.

Author

Mollett, Guy, Hinckel, Bruno C Bremer, Bhattacharyya, Tapan, Marlais, Tegwen, Singh, Om Prakash, Mertens, Pascal, Falconar, Andrew K, El-Safi, Sayda, Sundar, Shyam, and Miles, Michael A

Subjects
LEISHMANIASIS diagnosis, DISEASE relapse, ANTIGENS, COMMUNICABLE diseases, DIAGNOSTIC reagents & test kits, ENZYME-linked immunosorbent assay, IMMUNOGLOBULINS, LEISHMANIASIS, RECOMBINANT proteins, POINT-of-care testing, TREATMENT effectiveness, EVALUATION
Abstract

Background Visceral leishmaniasis (VL), caused by the Leishmania donovani complex, is a fatal, neglected tropical disease that is targeted for elimination in India, Nepal, and Bangladesh. Improved diagnostic tests are required for early case detection and for monitoring the outcomes of treatments. Previous investigations using Leishmania lysate antigen demonstrated that the immunoglobulin (Ig) G1 response is a potential indicator of a patient's clinical status after chemotherapy. Methods IgG1 or IgG enzyme-linked immunosorbent assays (ELISAs) with rK39 or lysate antigens and novel IgG1 rK39 rapid diagnostic tests (RDTs) were assessed with Indian VL serum samples from the following clinical groups: paired pre- and postchemotherapy (deemed cured); relapsed; other infectious diseases; and endemic, healthy controls. Results With paired pre- and post-treatment samples (n = 37 pairs), ELISAs with rK39- and IgG1-specific conjugates gave a far more discriminative decrease in post-treatment antibody responses when compared to IgG (P <.0001). Novel IgG1 rK39 RDTs provided strong evidence for decreased IgG1 responses in patients who had successful treatment (P <.0001). Furthermore, both IgG1 rK39 RDTs (n = 38) and ELISAs showed a highly significant difference in test outcomes between cured patients and those who relapsed (n = 23; P <.0001). RDTs were more sensitive than corresponding ELISAs. Conclusions We present strong evidence for the use of IgG1 in monitoring treatment outcomes in VL, and the first use of an IgG1-based RDT using the rK39 antigen for the discrimination of post-treatment cure versus relapse in VL. Such an RDT may have a significant role in monitoring patients and in targeted control and elimination of this devastating disease. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

44. Rapid identification of OXA-48 and OXA-163 subfamily in carbapenem resistant gram-negative bacilli with a novel immunochromatographic lateral flow assay.

Author

UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique, Pasteran, Fernando, Denorme, Laurence, Ote, Isabelle, Gomez, Sonia, De Belder, Denise, Glupczynski, Gerald, Bogaerts, Pierre, Ghiglione, Barbara, Power, Pablo, Mertens, Pascal, Corso, Alejandra, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Laboratoire de biologie clinique, Pasteran, Fernando, Denorme, Laurence, Ote, Isabelle, Gomez, Sonia, De Belder, Denise, Glupczynski, Gerald, Bogaerts, Pierre, Ghiglione, Barbara, Power, Pablo, Mertens, Pascal, and Corso, Alejandra

Abstract

We assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allows rapid (less than 4 minutes) and reliable direct confirmation of OXA-163/48-like enzymes (100% sensitivity and specificity) from culture colonies recovered from both, solid media and spiked blood culture bottles.

Published
2016

45. Experiences and Lessons from a Multicountry NIDIAG Study on Persistent Digestive Disorders in the Tropics

Author

Becker, Sören L., primary, Yap, Peiling, additional, Horié, Ninon S., additional, Alirol, Emilie, additional, Barbé, Barbara, additional, Bhatta, Nisha K., additional, Bhattarai, Narayan R., additional, Bottieau, Emmanuel, additional, Chatigre, Justin K., additional, Coulibaly, Jean T., additional, Fofana, Hassan K. M., additional, Jacobs, Jan, additional, Karki, Prahlad, additional, Khanal, Basudha, additional, Knopp, Stefanie, additional, Koirala, Kanika, additional, Mahendradhata, Yodi, additional, Mertens, Pascal, additional, Meyanti, Fransiska, additional, Murhandarwati, Elsa H., additional, N’Goran, Eliézer K., additional, Peeling, Rosanna W., additional, Pradhan, Bickram, additional, Ravinetto, Raffaella, additional, Rijal, Suman, additional, Sacko, Moussa, additional, Saye, Rénion, additional, Schneeberger, Pierre H. H., additional, Schurmans, Céline, additional, Silué, Kigbafori D., additional, Steinmann, Peter, additional, van Loen, Harry, additional, Verdonck, Kristien, additional, van Lieshout, Lisette, additional, von Müller, Lutz, additional, Yao, Joel A., additional, Boelaert, Marleen, additional, Chappuis, François, additional, Polman, Katja, additional, and Utzinger, Jürg, additional

Published
2016
Full Text
View/download PDF

46. Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay

Author

Pasteran, Fernando, primary, Denorme, Laurence, additional, Ote, Isabelle, additional, Gomez, Sonia, additional, De Belder, Denise, additional, Glupczynski, Youri, additional, Bogaerts, Pierre, additional, Ghiglione, Barbara, additional, Power, Pablo, additional, Mertens, Pascal, additional, and Corso, Alejandra, additional

Published
2016
Full Text
View/download PDF

48. Severity of Chagasic Cardiomyopathy Is Associated With Response to a Novel Rapid Diagnostic Test for Trypanosoma cruzi TcII/V/VI.

Author

Bhattacharyya, Tapan, Messenger, Louisa A, Bern, Caryn, Mertens, Pascal, Gilleman, Quentin, Zeippen, Nicolas, Hinckel, Bruno C Bremer, Murphy, Niamh, Gilman, Robert H, and Miles, Michael A

Subjects
CARDIOMYOPATHIES, ANTIGENS, TRYPANOSOMIASIS, CHI-squared test, CHRONIC diseases, ELECTROCARDIOGRAPHY, ENZYME-linked immunosorbent assay, CARDIAC patients, IMMUNOGLOBULINS, PROTOZOA, SEVERITY of illness index, DISEASE exacerbation, DISEASE progression, VENTRICULAR ejection fraction, PROGNOSIS, DIAGNOSIS
Abstract

Background Trypanosoma cruzi causes Chagas disease in the Americas. The outcome of infection ranges from lifelong asymptomatic status to severe disease. Relationship between T. cruzi lineage (TcI-TcVI) infection history and prognosis is not understood. We previously described peptide-based lineage-specific enzyme-linked immunosorbent assay (ELISA) with trypomastigote small surface antigen (TSSA). Methods A novel rapid diagnostic test (RDT; Chagas Sero K-SeT) that incorporates a peptide that corresponds to the TSSA II/V/VI common epitope was developed and validated by comparison with ELISA. Patients from Bolivia and Peru, including individuals with varying cardiac pathology, and matched mothers and neonates, were then tested using Chagas Sero K-SeT. Results Chagas Sero K-SeT and ELISA results, with a Bolivian subset of cardiac patients, mothers, and neonates, were in accord. In adult chronic infections (n = 121), comparison of severity class A (no evidence of Chagas cardiomyopathy) with class B (electrocardiogram suggestive of Chagas cardiomyopathy) and class C/D (decreased left ventricular ejection fraction; moderate/severe Chagas cardiomyopathy) revealed a statistically significant increase in Chagas Sero K-SeT reactivity with increasing severity (χ2 for trend, 7.39; P =.007). In Peru, Chagas Sero K-SeT detected the sporadic TcII/V/VI infections. Conclusions We developed a low cost RDT that can replace ELISA for identification of TSSA II/V/VI immunoglobulin G. Most importantly, we show that response to this RDT is associated with severity of Chagas cardiomyopathy and thus may have prognostic value. Repeated challenge with T. cruzi infection may both exacerbate disease progression and boost the immune response to the TSSApep-II/V/VI epitope. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

49. Erratum to: Diagnosis of neglected tropical diseases among patients with persistent digestive disorders (diarrhoea and/or abdominal pain ≥14 days): a multi-country, prospective, non-experimental case–control study

Author

Polman, Katja, primary, Becker, Sören L., additional, Alirol, Emilie, additional, Bhatta, Nisha K., additional, Bhattarai, Narayan R., additional, Bottieau, Emmanuel, additional, Bratschi, Martin W., additional, Burza, Sakib, additional, Coulibaly, Jean T., additional, Doumbia, Mama N., additional, Horié, Ninon S., additional, Jacobs, Jan, additional, Khanal, Basudha, additional, Landouré, Aly, additional, Mahendradhata, Yodi, additional, Meheus, Filip, additional, Mertens, Pascal, additional, Meyanti, Fransiska, additional, Murhandarwati, Elsa H., additional, N’Goran, Eliézer K., additional, Peeling, Rosanna W., additional, Ravinetto, Raffaella, additional, Rijal, Suman, additional, Sacko, Moussa, additional, Saye, Rénion, additional, Schneeberger, Pierre H. H., additional, Schurmans, Céline, additional, Silué, Kigbafori D., additional, Thobari, Jarir A., additional, Traoré, Mamadou S., additional, van Lieshout, Lisette, additional, van Loen, Harry, additional, Verdonck, Kristien, additional, von Müller, Lutz, additional, Yansouni, Cédric P., additional, Yao, Joel A., additional, Yao, Patrick K., additional, Yap, Peiling, additional, Boelaert, Marleen, additional, Chappuis, François, additional, and Utzinger, Jürg, additional

Published
2015
Full Text
View/download PDF

50. Molecular and culture-based diagnosis ofClostridium difficileisolates from Côte d'Ivoire after prolonged storage at disrupted cold chain conditions

Author

Becker, Sören L., primary, Chatigre, Justin K., additional, Coulibaly, Jean T., additional, Mertens, Pascal, additional, Bonfoh, Bassirou, additional, Herrmann, Mathias, additional, Kuijper, Ed J., additional, N'Goran, Eliézer K., additional, Utzinger, Jürg, additional, and von Müller, Lutz, additional

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results