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Knowledge of anatomical variants of the vasculo-nervous elements of the internal acustic meatus helps to understand the pathophysiology of some patterns of hearing disturbances. The purpose of this study was to clarify the spacial relationship of the labyrinthine artery and the vascular loop of the anterior inferior cerebellar artery, based on the data issued from anatomical dissections under operating microscope on the colored gelatin-injected specimens. All the specimens dissected presented a vascular loop formed by the anterior inferior cerebellar artery or the labyrinthine artery. The origin, number and trajects of the labyrinthine artery presents a wide variety, even in the case of the same individual (left-right). [ABSTRACT FROM AUTHOR]

During antigen-driven responses, B cells can differentiate at extra-follicular (EF) sites or initiate germinal centers (GCs) in processes that involve interactions with T cells. Here, we examined the roles of interleukin (IL)-2 secreted by T helper (Th) cells during cognate interactions with activated B cells. IL-2 boosted the expansion of EF plasma cells and the secretion of low-mutated immunoglobulin G (IgG). Conversely, genetically disrupting IL-2 expression by CD4 + T cells, or IL-2 receptor (CD25) expression by B cells, promoted B cell entry into the GC and high-affinity antibody secretion. Mechanistically, IL-2 induced early mTOR activity, expression of the transcriptional regulator IRF4, and metabolic changes in B cells required to form Blimp-1-expressing plasma cells. Thus, T cell help via IL-2 regulates an mTOR-AKT-Blimp-1 axis in activated B cells, providing insight into the mechanisms that determine EF versus GC fates and positioning IL-2 as an early switch controlling plasma cell versus GC B cell commitment., Competing Interests: Declaration of interests S.C. has consulted for GSK, JP Morgan, Citi, Morgan Stanley, Avalia NZ, Nutcracker Therapeutics, University of California, California State Universities, United Airlines, Adagio, Sanofi, and Roche. W.R.S. is inventor on patent applications related to immunogens in this manuscript filed by Scripps and IAVI. W.R.S. is an employee of Moderna, Inc. S.B. is a current employee of VIR Biotechnology and may possess shares of VIR Biotechnology., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: When conventional trans-venous CS lead placement fails, trans-septal endocardial left ventricle lead placement is an alternative technique used to capture the left ventricle endocardially; however, its use is limited due to a lack of evidence, practice uptake, and clinical trials., Methods: In this single-center cohort study, we evaluated the efficiency of the procedure, post-procedural complication rate, rate of thromboembolic events, overall survival rate, and changes in the echocardiographic parameters, brain natriuretic peptide (BNP) level, and New York Heart Association (NYHA) class, both before and after TSLV lead implantation., Results: The TSLV lead implant is safe and improves EF, LVEDV, LVESV, and LVIDd. It significantly reduces the NTproBNP levels and the NYHA class; however, the rate of stroke incidence remains high, at 9%., Conclusions: We demonstrated a high success rate of trans-septal left ventricular endocardial lead implantation, LV reverse remodeling was noted, and patients had a favorable clinical response; however, there was an increased risk of systemic embolization after the trans-septal LV lead implant.

Introduction: The efficacy of de novo cardiac resynchronisation therapy (CRT) in patients with heart failure (HF), left ventricular systolic dysfunction (LVSD), and a broad QRS morphology is well established. However, the optimal stage for upgrading patients with existing pacemakers (PPMs) or implantable cardioverter-defibrillators (ICDs) and HF with high-burden right ventricular (RV) pacing remains uncertain. Thus, this multicentre retrospective analysis compared patients with pre-existing PPMs or ICDs who underwent CRT upgrades to investigate the appropriate stage for CRT implantation in these patients and to assess the validity of treating both PPM and ICD recipients under the same recommendation level in the current guidelines. Materials and Methods: A total of 151 participants underwent analysis in this study, comprising 93 upgrades to cardiac resynchronisation therapy with pacemaker (CRT-P) and 58 upgrades to cardiac resynchronisation therapy with defibrillator (CRT-D) across three centres in the UK. The aim of the study was to investigate the safety and efficacy of upgrading to CRT from an existing conventional pacemaker or an ICD in the context of high-burden RV pacing. The analysis was conducted separately for each group, assessing changes in echocardiographic parameters, functional New York Heart Association (NYHA) class, and procedure-related complications. Results: The PPM group had a higher percentage RVP burden compared to the ICD group. Post-upgrade, NYHA functional class and EF and LV volumes improved in both groups; however, the response to an upgrade from a pacemaker was greater compared to an upgrade from an ICD. Post-procedural complication risks were similar across the two subgroups but significantly higher compared to de novo implantation. Conclusions: Within the CRT-P subgroup, participants exhibited better responses than their CRT-D counterparts, evident both in echocardiographic improvements and clinical outcomes. Furthermore, patients with non-ischemic cardiomyopathy (NICM) were better responders than those with ischaemic cardiomyopathy. These findings suggest that international guidelines should consider approaching each subgroup separately in the future.

Purpose: Prostate cancer (PC) is the second leading cause of cancer and the fifth cause of cancer-related death. This manuscript aims to determine the incidence, mortality, and Disability Adjusted Life Years (DALYs) trends of PC in the last 30 years in Latin America and Mexico., Methods: We performed a cross-sectional analysis of a publicly available data set. Data regarding the burden of prostate cancer in 20 Latin-American countries, and the 32 states of Mexico, were retrieved from the Global Burden of Disease Study 2019. Collected information included incidence and mortality rates (per 100,000), as well as the DALYs as absolute numbers and rates (per 100,000) and the annual rates of change in rates from 1990 to 2019., Results: In Latin America in males aged 55 years or older, the mean incidence rate was 344 cases per 100,000. The number of deaths attributable to prostate cancer observed was 67,110 and the mean mortality rate was 210 per 100,000. The overall burden of disease was 1,120,709 DALYs and the contribution of years of life lost (YLL) was 91.7% ([Formula: see text] = 1,027,946). Mexico presented an incidence rate (279.6) and mortality (99.1) rate (per /100 thousand). In Mexico, 13 states had a DALYs' rate above the national mean (883 per 100,000) and the highest burden (1360 DALYs/100,000) were documented in the state of Guerrero (Southwestern Mexico)., Conclusion: Only two Latin-American countries (Brazil and Colombia) and eight states of Mexico showed a decreased trend about the rate of change of DALYs in the last 30 years., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

Introduction: Introduction: CD36 is a membrane protein that functions as a lingual receptor for lipids. The soluble CD36 fraction (sCD36) may correlate oral fatty acid fat taste sensitivity to body mass index (BMI) and adiposity. Objectives: to determine if the oral fatty acid taste sensitivity in healthy young individuals of both sexes is related to serum sCD36 levels, adiposity and BMI. Methods: a cross-sectional study was conducted in 72 healthy young individuals (18-25 years). Serum sCD36 was quantified for all subjects. Oral fatty acid taste sensitivity was determined using an ascending series of the three-alternate forced choice methodology. Additionally, BMI was calculated using anthropometry, and adiposity was determined by bioelectric impedance analysis. Results: there was a positive correlation between BMI and the oral fatty acid taste sensitivity threshold (r = 0.277, p < 0.05) and a negative correlation between BMI and serum sCD36 levels (r = -0.035, p < 0.01). Adiposity negatively correlated with the sCD36 levels only in women (r = -0.359, p < 0.05). The threshold for oral sensitivity to fatty acids in overweight individuals was 1.0 (IQR 1.16) mM vs 0.2 (IQR 0.29) mM in healthy weight individuals (p < 0.05), while sCD36 levels were 26.1 pg/ml (IQR 32.9) and 77.97 pg/ml (IQR 560.66) in overweight and normal weight individuals, respectively (p < 0.05). Conclusions: BMI positively correlates with the oral sensitivity threshold of fatty acids and negatively correlates with serum sCD36 levels. The threshold of oral sensitivity to fatty acids was significantly higher in overweight subjects, while sCD36 levels were significantly higher in the group of normal weight individuals.

Background: Dengue and chikungunya infections are transmitted by the Aedes aegypti vector; they can affect the pregnant woman and her gestation product., Objective: To identify if dengue and chikungunya infections during pregnancy are factors associated with the presence of obstetric complications, in Colima, Mexico., Methods: We included in this study 45 patients with obstetric complications and 90 without complications, selected by a two-stage sampling. We collected the sociodemographic, anthropometric, gynecological, and obstetric complications’ data. Descriptive statistics, non-parametric significance tests and crude and adjusted risk estimates (odds ratio [OR] and 95% confidence intervals [95% CI]) were calculated., Results: Out of 135 patients included, 15 had a diagnosis of dengue and 20 of chikungunya. There were obstetric complications in 60% of infected patients with chikungunya, 46.7% with dengue and 26% of non-infected (p = 0.007). Chikungunya infection (adjusted OR = 1.6) and antecedents of abortion and cesarean section (adjusted OR = 2.2) were independent risk factors associated with the presence of complications. Dengue had an adjusted OR = 1.3 without a statistically significant difference., Conclusions: Chikungunya infection and antecedents of abortion and cesarean section are risk factors associated with obstetric complications. Their timely detection is a priority as a preventive strategy.

Nopal is used in Mexico as both food and traditional medicine for metabolic diseases. Our aim was to analyze the effect of the chronic administration of mucilage fiber extracted from nopal ( Opuntia ficus indica ; 500 mg/kg body weight per day) on male Wistar rats on a high-fructose diet (HFD). After which three groups were administered one of the following for 30 days: whole-fresh nopal mixed in water, mucilage, and control. Metabolic and hemodynamic parameters (triglycerides, cholesterol, fasting glucose, oral glucose tolerance test, blood pressure, and abdominal circumference) were determined. Rats administered nopal and mucilage had lower levels of triglycerides and diastolic arterial pressure than control, but only nopal had significant differences. Furthermore, systolic and diastolic pressure were higher in the control group. Thus, whole nopal and mucilage improve metabolic parameters in rats fed a HFD.

Research on the toxicity of nanoparticles has developed over recent years due to their increasing prevalence in common everyday materials. Various nanoparticles have been reported to promote and induce mucus secretion, which could potentially lead to airway damages and respiratory complications. Lanthanum strontium manganite (LSM) is a nanoparticle widely used in solar oxidized fuel cells (SOFCs) due to its high electrical conductivity, high electrochemical activity for O 2 reduction reaction, high thermal stability and compatibility of SOFC electrolytes, and most importantly, its microstructural stability and long-term performance. Very few studies have been conducted on LMS's toxicity, thus its effect on airway cells was investigated in this study. After treating trachea cells with increasing concentrations of LSM ranging up to 500 μg/ml, we found that it has a moderate effect on cell viability, ROS production, cytochrome C, and caspase 3 expression. Despite its minimal impact on stated apoptosis-inducing characteristics, LSM illustrated an inhibiting effect on mucus secretion. We obtained a decreasing trend in mucus secretion with an increased concentration of the LSM treatment. Overall, LSM's advancement in SOFCs necessitated a toxicity study, and although it does not show a significant toxicity to trachea cells, LSM reduces mucus secretion, and can potentially interfere with airway clearance.

Nanotoxicity studies associated with various nanoparticles (NPs) have attracted intense research interest due to the broader applications of nanoparticles in our daily lives. The exposure of nanoparticles can lead to hypersecretion and accumulation of airway mucus which are closely associated with many respiratory diseases. Titanium dioxide (TiO 2 ), one of the PM10 components, is a major NP that is widely utilized in many commercial products. Our previous study established the connection between induced airway mucus secretion and TiO 2 NPs. However, the countermeasure to reduce the harmful effects of TiO 2 NPs, especially airway mucus secretion, remains unexplored. One of the potential candidates to reduce airway mucus secretion is cerium oxide (CeO 2 ) NPs. It has been reported that CeO 2 NPs can protect cells by diminishing ROS and inflammatory responses. Herein, our study shows that CeO 2 NPs are able to reduce cytosolic Ca 2+ changes and mitochondrial damage caused by TiO 2 NPs. Our results provide the evidence that hypersecretion of mucus and apoptosis progression induced by TiO 2 NPs can be attenuated by CeO 2 NPs. This study highlights the potential capacity of CeO 2 NPs as a supplementary material for TiO 2 NPs applications in the future., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Previous studies have indicated that vanilloid receptor (VR1) mRNA is expressed in muscle fibers. In this study, we evaluated the functional effects of VR1 activation. We measured caffeine-induced contractions in bundles of the extensor digitorum longus muscle of Rana pipiens. Isometric tension measurements showed that two VR1 agonists, capsaicin (CAP) and N-arachidonoyl-dopamine (NADA), reduced muscle peak tension to 57 ± 4 % and 71 ± 3% of control, respectively. The effect of CAP was partially blocked by a VR1 blocker, capsazepine (CPZ), but the effect of NADA was not changed by CPZ. Because NADA is able to act on cannabinoid receptors, which are also present in muscle fibers, we tested the cannabinoid antagonist AM281. We found that AM281 antagonized both CAP and NADA effects. AM281 alone reduced peak tension to 80 ± 6 % of control. With both antagonists, the CAP effect was completely blocked, and the NADA effect was partially blocked. These results provide pharmacological evidence of the functional presence of the VR1 receptor in fast skeletal muscle fibers of the frog and suggest that capsaicin and NADA reduce tension by activating both cannabinoid and vanilloid receptors.

In contrast to fast-twitch skeletal muscle fibers of the chicken, slow-twitch fibers are fatigue-resistant. In fast fibers, the fatigue process has been related to K(ATP) channels. In the present study, we investigated the action of glibenclamide (an anti-diabetic sulphonylurea that acts on K(ATP) channels) on fatigued slow skeletal muscle, studying twitch and tetanus tension after inducing the muscle to fatigue by continuous electrical stimulation. Our results showed that glibenclamide (150 μM) increased post-fatigue twitch tension by about 25% with respect to the fatigued condition (P < 0.05). In addition, glibenclamide (150 μM) increased post-fatigue tetanic tension (83.61 ± 15.7% in peak tension, and 85.0 ± 19.0% in tension-time integral, P = 0.02, and 0.04, respectively; n = 3). Moreover, after exposing the muscle to a condition that inhibits mitochondrial ATP formation in order to activate K(ATP) channels with cyanide (10 mM), tension also diminished, but in the presence of glibenclamide the effect produced by cyanide was abolished. To determine a possible increase in intracellular calcium concentration, the effects of glibenclamide on caffeine-evoked contractures were explored. After muscle pre-incubation with glibenclamide (150 μM), tension of caffeine-evoked contractures increased (6.5 ± 1.5% in maximal tension, and 5.9 ± 3.8% in tension-time integral, P < 0.05). These results suggest a possible role of K(ATP) channels in the fatigue process, since glibenclamide increases twitch and tetanus tension in fatigued slow muscle of the chicken and during metabolic inhibition, possibly by increasing intracellular calcium.

The effect of cannabinoids on caffeine contractures was investigated in slow and fast skeletal muscle fibers using isometric tension recording. In slow muscle fibers, WIN 55,212-2 (10 and 5 microM) caused a decrease in tension. These doses reduced maximum tension to 67.43 +/- 8.07% (P = 0.02, n = 5) and 79.4 +/- 14.11% (P = 0.007, n = 5) compared to control, respectively. Tension-time integral was reduced to 58.37 +/- 7.17% and 75.10 +/- 3.60% (P = 0.002, n = 5), respectively. Using the CB(1) cannabinoid receptor agonist ACPA (1 microM) reduced the maximum tension of caffeine contractures by 68.70 +/- 11.63% (P = 0.01, n = 5); tension-time integral was reduced by 66.82 +/- 6.89% (P = 0.02, n = 5) compared to controls. When the CB(1) receptor antagonist AM281 was coapplied with ACPA, it reversed the effect of ACPA on caffeine-evoked tension. In slow and fast muscle fibers incubated with the pertussis toxin, ACPA had no effect on tension evoked by caffeine. In fast muscle fibers, ACPA (1 microM) also decreased tension; the maximum tension was reduced by 56.48 +/- 3.4% (P = 0.001, n = 4), and tension-time integral was reduced by 57.81 +/- 2.6% (P = 0.006, n = 4). This ACPA effect was not statistically significant with respect to the reduction in tension in slow muscle fibers. Moreover, we detected the presence of mRNA for the cannabinoid CB(1) receptor on fast and slow skeletal muscle fibers, which was significantly higher in fast compared to slow muscle fiber expression. In conclusion, our results suggest that in the slow and fast muscle fibers of the frog cannabinoids diminish caffeine-evoked tension through a receptor-mediated mechanism.

We report the case of two patients suffered from cholestatic jaundice occurred 3-4 weeks after starting ticlopidine therapy. In both cases the diagnosis was made by ruling out any other known cause of acute hepatitis or cholestasis. One patient underwent liver biopsy, which showed a typical intralobular cholestatic pattern and a slight lymphocytic infiltration of the portal tracts. The other patient, a 29 year-old woman, was taking ticlopidine as the sole drug, further to an ischemic stroke occurred while she was taking oral contraceptives; she presented a diffuse itchy dermatitis, fever and slight eosinophilia besides cholestasis. In both patients ticlopidine was discontinued and liver tests returned to normal values within 4-8 weeks; no rechallenge was attempted and ticlopidine was replaced with another antiplatelet drug. To the best of our knowledge 19 cases of ticlopidine-related cholestatic disease have been described so far in the literature. Its pathogenesis is still unknown, although some clinical findings and experimental results from patients with acute enteropathy or agranulocytosis induced by ticlopidine suggest that the drug may act through a toxic mechanism, perhaps mediated by prostaglandins.