1. Investigation of Folate-Functionalized Magnetic-Gold Nanoparticles Based Targeted Drug Delivery for Liver: In Vitro, In Vivo and Docking Studies.
- Author
-
Alnasraui AHF, Joe IH, and Al-Musawi S
- Subjects
- Animals, Humans, Cell Line, Tumor, Mice, Drug Delivery Systems, Magnetite Nanoparticles chemistry, Magnetite Nanoparticles therapeutic use, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Liver Neoplasms drug therapy, Liver Neoplasms metabolism, Liver Neoplasms pathology, Liver drug effects, Liver metabolism, Metal Nanoparticles chemistry, Metal Nanoparticles therapeutic use, Mice, Nude, Folic Acid chemistry, Folic Acid pharmacology, Gold chemistry, Gold pharmacology, Curcumin pharmacology, Curcumin chemistry, Molecular Docking Simulation
- Abstract
Magnetic nanoparticles used for targeted drug administration present a promising approach in cancer treatment owing to its notable advantages, such as targeted and enhanced encapsulation ability and improved bio protection compared with conventional drug delivery methods. Au shell-iron core nanoparticles (Fe
3 O4 @Au) were manufactured by a chemical process, coated with dextran to encapsulate curcumin, and functionalized for precision drug delivery using folic acid to combat liver cancer. Dynamic light scattering, scanning electron microscopy, transmission electron microscopy, vibrational spectroscopy, and magnetometry were applied to assess the synthesis of the Fe3 O4 @Au-DEX-CU-FA compound. The mean size, zeta potential, and polydispersity of Fe3 O4 @Au-DEX-CU-FA were 63.3 ± 2.33 nm, -68.3 ± 1.78 mV, and 0.041 ± 0.008, respectively. Molecular docking models were created to examine the relationship between Fe3 O4 @Au-CU and BCL-XL, BAK, and to identify potential binding sites. The loading efficiency and release profile tests examined the medication delivery system's ability. MTT assay was subsequently utilized to determine the optimal dosage and therapeutic efficacy of Fe3 O4 @Au-DEX-CU-FA on cancer SNU-449 and healthy THLE-2 cell lines. Flow cytometry demonstrated that Fe3 O4 @Au-DEX-CU-FA effectively induced cancer cell death. Fe3 O4 @Au-DEX-FA showed a regulated release profile of free curcumin at 37 °C and pH values of 7.4 and 5.4. Real-time PCR revealed increased BAK expression and decreased BCL-XL expression. Nude tumor-bearing mice were used for in vivo experiments. Fe3 O4 @Au-DEX-CU-FA treatment dramatically reduced the swelling size compared with free CU and control treatments. It also resulted in a longer lifespan, expanded splenocyte proliferation, increased IFN-γ levels, and decreased IL-4 levels. The regular cells showed no cytotoxic effect compared with the cancer type, confirming that Fe3 O4 @Au-DEX-CU-FA maintained its potent anticancer actions. The data suggests that Fe3 O4 @Au-DEX-CU-FA possesses a promising potential as a therapeutic agent for combating tumors.- Published
- 2024
- Full Text
- View/download PDF