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6 results on '"Meza, Denisse"'

3. UBE4B Protein Couples Ubiquitination and Sorting Machineries to Enable Epidermal Growth Factor Receptor (EGFR) Degradation*

Author

Sirisaengtaksin, Natalie, Gireud, Monica, Yan, Qing, Kubota, Yoshihisa, Meza, Denisse, Waymire, Jack C, Zage, Peter E, and Bean, Andrew J

Subjects
Biochemistry and Cell Biology, Biological Sciences, Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance, Cell Membrane, Endopeptidases, Endosomal Sorting Complexes Required for Transport, Endosomes, ErbB Receptors, HeLa Cells, Humans, Membrane Proteins, Phosphoproteins, Protein Interaction Domains and Motifs, Protein Structure, Tertiary, Protein Transport, Proteolysis, Signal Transduction, Tumor Suppressor Proteins, Ubiquitin Thiolesterase, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitination, Deubiquitination, E3 Ubiquitin Ligase, Endocytosis, Protein Sorting, ESCRT, USP8, Hela Cells, Chemical Sciences, Medical and Health Sciences, Biochemistry & Molecular Biology, Biological sciences, Biomedical and clinical sciences, Chemical sciences
Abstract

The signaling of plasma membrane proteins is tuned by internalization and sorting in the endocytic pathway prior to recycling or degradation in lysosomes. Ubiquitin modification allows recognition and association of cargo with endosomally associated protein complexes, enabling sorting of proteins to be degraded from those to be recycled. The mechanism that provides coordination between the cellular machineries that mediate ubiquitination and endosomal sorting is unknown. We report that the ubiquitin ligase UBE4B is recruited to endosomes in response to epidermal growth factor receptor (EGFR) activation by binding to Hrs, a key component of endosomal sorting complex required for transport (ESCRT) 0. We identify the EGFR as a substrate for UBE4B, establish UBE4B as a regulator of EGFR degradation, and describe a mechanism by which UBE4B regulates endosomal sorting, affecting cellular levels of the EGFR and its downstream signaling. We propose a model in which the coordinated action of UBE4B, ESCRT-0, and the deubiquitinating enzyme USP8 enable the endosomal sorting and lysosomal degradation of the EGFR.

Published
2014

5. Detection of Tritrichomonas foetusby RT-rtPCR in pooled bovine preputial washings

Author

Schroeder, Megan E., Meza, Denisse, Shah, Rohan, Leyva-Baca, Ivan, Conrad, Rick, and Ferro, Pamela J.

Abstract

Trichomonosis is a venereal disease of cattle caused by the protozoan Tritrichomonas foetus. T. foetusinfection in cattle herds can be economically costly for cattle producers; therefore, testing is important for detection of the agent. Given that bulls are considered to be subclinical carriers of T. foetus, it is important to detect T. foetusinfection prior to movement and/or breeding season. We have described previously the development of an updated set of PCR primers and probes that offer increased sensitivity of T. foetusdetection in preputial washings collected in PBS by utilizing reverse-transcription real-time PCR (RT-rtPCR) that targets the 5.8S ribosomal RNA of the T. foetusorganism. Here, we report improvements in the updated RT-rtPCR reagents as well as the evaluation of testing of pooled preputial washings. We found that up to 5 preputial washings can be pooled, similar to routine testing practices (InPouch culture), without reducing the sensitivity of detection of T. foetus.

Published
2023
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6. Green silver nanoparticles display protection against Clavibacter michiganensissubsp. michiganensisin tomato plants (Solanum lycopersicumL.)

Author

Mercado-Meza, Denisse Yatzely, Guevara-González, Ramón Gerardo, Esquivel, Karen, Carbajal-Valenzuela, Ireri, and Avila-Quezada, Graciela Dolores

Abstract

•Clavibacter michiganensisis a bacteriun causing canker disease in tomato•Green nanoparticles based on moringa extracts are promising tools in phytopathology•AgNPs produced with moringa extracts reduced in 86.29 % of Clavibacterdisease•Systemic aquired resistance is suggested as important mechanism induced by Mo-AgNPs

Published
2023
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