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This contribution provides the first karyotype description of Hemidactylus mercatorius and discusses the interspecific chromosome diversification in the genus. Chromosomal analysis was performed on samples from different Malagasy populations using standard karyotyping, Ag-NOR staining, and banding methods (sequential C-banding + Giemsa, + Chromomycin A3, +4′,6-diamidino-2-phenylindole). Irrespective of sex or sampling locality, H. mercatorius shows a karyotype of 2n = 42 with metacentric (1, 18–21), submetacentric (4), subtelocentric (5, 11), and acrocentric pairs (all the remaining pairs). There was no heteromorphic chromosome pair and no clear distinction between macro- and microchromosomes. NORs were localised close to the centromeres of a medium acrocentric pair (14). Heterochromatic blocks were identified on the telomeric and centromeric regions of most chromosome pairs. A comparison with the karyotype of H. mabouia highlights that the different morphology of several chromosome pairs clearly distinguishes the two species, contrasting the previously proposed synonymy. The differences between the karyotypes of H. mercatorius and H. mabouia concern the number of biarmed and acrocentric elements, suggesting the occurrence of several chromosome inversions. Considering all the available karyotype data on Hemidactylus and its sister genus Cyrtodactylus, it is possible to advance an evolutionary hypothesis on their chromosomal evolution, starting from a common ancestor with 2n = 48 and all acrocentric elements. From this ancestral condition, the karyotype diversification in the two genera has been prevalently characterised by a progressive accumulation of fusions and inversions which have reduced the total chromosome count and increased the number of biarmed chromosomes. [ABSTRACT FROM AUTHOR]

Speciation is the fundamental process that underlies the diversification of species. Different steps of speciation, such as reproductive isolation or ecological niche separation, can evolve in different orders and over different time scales. The two subspecies of the green whip snake, Hierophis viridiflavus viridiflavus and Hierophis viridiflavus carbonarius , sometimes considered as separate species, are ideal to study speciation because they diverged in isolated glacial refugia. So far, little is known about whether these two subspecies are reproductively isolated from each other. Furthermore, it is unknown whether the common melanism of the subspecies H. v. carbonarius might be genetically encoded. We quantified gene flow along two transects across the contact zone of the two subspecies using double-digest restriction site-associated DNA. Our analyses implicated the existence of two well-defined clades that experience substantial gene flow along a contact zone that is ~300 km wide. We detected a clear association between the genetic assignment and the quantitative extent of melanism within the contact zone, which is likely to be genetically polygenic. Given the level of gene flow, it seems that strong postzygotic reproductive barriers have not evolved. We conclude that the two subspecies represent evolutionarily significant units for conservation and suggest treating them taxonomically as subspecies. [ABSTRACT FROM AUTHOR]

Simple Summary: Several life-history traits of Mediterranean sperm whales are little explored. We present new original data on the relationships between age and body length and age at maturity of individuals stranded along the Italian coast. We found that Mediterranean male sperm whales attain sexual maturity at 10 years and for the same body length class are older than Atlantic ones. Our finding of a Mediterranean pregnant female of only 6.5 m of body length and an assessed age of 24–26 years is particularly noteworthy, as the females of this species reach sexual maturity at about 9 meters total length and 9 years of age in other marine areas. Two different hypotheses can be advanced to explain this difference in size, based on either ecological or genetic factors. The first is based on a lesser energy intake of Mediterranean individuals compared to Atlantic ones, mostly due to a smaller prey size and less trophic conditions. The second hypothesis concerns a low genetic diversity and mating among closely related individuals. Considering its low genetic diversity, the Mediterranean sperm whale population should be the target of focused conservation efforts as it might be relatively less responsive to environmental changes. We investigated the relationship between age and body length, and age at sexual maturity of Physeter macrocephalus individuals stranded along the Italian coast. Our molecular analysis shows that all our samples belong to the C.001.002 haplotype, shared between Atlantic and Mediterranean populations. We show that males attain sexual maturity at 10 years, similar to those from other marine areas. However, considering the same body length class, Mediterranean males are older than Atlantic ones. Our finding of a Mediterranean pregnant female of only 6.5 m in length and an assessed age of 24–26 years is particularly noteworthy, considering that females reach sexual maturity at about 9 years and 9 m of total length in other regions. Comparing our results with the literature data, we highlight the positive correlation between lifespan, adult body length and weight of males from the Mediterranean and Atlantic Ocean. Regardless of whether the relatively small size of Mediterranean specimens is a consequence of an inbreeding depression or an adaptation to less favorable trophic conditions, we recommend to closely monitor this population from a conservation perspective. In fact, its low genetic diversity likely corresponds to a relatively limited ability to respond to environmental changes compared with other populations. [ABSTRACT FROM AUTHOR]

We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic identification of the samples that were used for cytogenetic analyses. A comparative cytogenetic analysis, with Giemsa's staining, Ag-NOR staining and sequential C-banding + Giemsa + CMA + DAPI was performed on eight species: Gephyromantis sp. Ca19, G. striatus (Vences, Glaw, Andreone, Jesu et Schimmenti, 2002), Mantidactylus (Chonomantis) sp. Ca11, M. (Brygoomantis) alutus (Peracca, 1893), M. (Hylobatrachus) cowanii (Boulenger, 1882), Spinomantis prope aglavei "North" (Methuen et Hewitt, 1913), S. phantasticus (Glaw et Vences, 1997) and S. sp. Ca3. Gephyromantis striatus, M. (Brygoomantis) alutus and Spinomantis prope aglavei "North" have a karyotype of 2n = 24 chromosomes while the other species show 2n = 26 chromosomes. Among the analysed species we detected differences in the number and position of telocentric elements, location of NOR loci (alternatively on the 6th, 7th or 10th pair) and in the distribution of heterochromatin, which shows species-specific patterns. Merging our data with those previously available, we propose a karyotype of 2n = 26 with all biarmed elements and loci of NORs on the 6th chromosome pair as the ancestral state in the whole family Mantellidae. From this putative ancestral condition, a reduction of chromosome number through similar tandem fusions (from 2n = 26 to 2n = 24) occurred independently in Mantidactylus Boulenger, 1895 (subgenus Brygoomantis Dubois, 1992), Spinomantis Dubois, 1992 and Gephyromantis Methuen, 1920. Similarly, a relocation of NORs, from the putative primitive configuration on the 6th chromosome, occurred independently in Gephyromantis, Blommersia Dubois, 1992, Guibemantis Dubois, 1992, Mantella Boulenger, 1882 and Spinomantis. Chromosome inversions of primitive biarmed elements likely generated a variable number of telocentric elements in Mantella nigricans Guibé, 1978 and a different number of taxa of Gephyromantis (subgenera Duboimantis Glaw et Vences, 2006 and Laurentomantis Dubois, 1980) and Mantidactylus (subgenera Brygoomantis, Chonomantis Glaw et Vences, 1994, Hylobatrachus Laurent, 1943 and Ochthomantis Glaw et Vences, 1994). [ABSTRACT FROM AUTHOR]