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Purpose: To develop a mathematical model that can predict the amount of refractive change caused by implantation of an intraocular lens (IOL) in a reversed position during cataract surgery. Methods: A theoretical mathematical formula based on the Gullstrand eye model was constructed to estimate the refractive change of the eye after implantation of a reversed IOL. The refractive change caused by implantation of the IOL in a reversed position was calculated based on the exchange of the anterior curvature with the posterior curvature of the IOL, and the lengthening of the distance between the IOL and the retina. In case of a three-piece IOL with angulation, the amount of refractive change was calculated based on its angle and the total refractive power of the eye, which is dependent on the focal length of the eye. Results: Calculated refractive change for one-piece IOLs was less than 0.10 diopter (D). For three-piece IOLs, the calculated refractive change makes the eye on average 0.77 D more myopic and can increase with the total refractive power of the patient's eye. The mathematical model was applied to seven previously published cases of reverse IOL implantation. Conclusions: This calculation demonstrates that with an upside-down IOL, there is a small refractive change in the one-piece IOL, including a toric IOL without angulation, but there can be a large refractive change in the three-piece IOL with angulation, especially using a higher power IOL or with a shorter axial length. [ J Refract Surg . 2023;39(5):326–331.]

PurposeTo compare convolutional neural network (CNN) analysis of en face vessel density images to gradient boosting classifier (GBC) analysis of instrument-provided, feature-based optical coherence tomography angiography (OCTA) vessel density measurements and OCT retinal nerve fiber layer (RNFL) thickness measurements for classifying healthy and glaucomatous eyes.DesignComparison of diagnostic approaches.MethodsA total of 130 eyes of 80 healthy individuals and 275 eyes of 185 glaucoma patients with optic nerve head (ONH) OCTA and OCT imaging were included. Classification performance of a VGG16 CNN trained and tested on entire en face 4.5×4.5-mm radial peripapillary capillary OCTA ONH images was compared to the performance of separate GBC models trained and tested on standard OCTA and OCT measurements. Five-fold cross-validation was used to test predictions for CNNs and GBCs. Areas under the precision recall curves (AUPRC) were calculated to control for training/test set size imbalance and were compared.ResultsAdjusted AUPRCs for GBC models were 0.89 (95% CI=0.82, 0.92) for whole image vessel density GBC, 0.89 (0.83, 0.92) for whole image capillary density GBC, 0.91 (0.88, 0.93) for combined whole image vessel and whole image capillary density GBC, and 0.93 (0.91, 095) for RNFL thickness GBC. The adjusted AUPRC using CNN analysis of en face vessel density images was 0.97 (0.95, 0.99) resulting in significantly improved classification compared to GBC OCTA-based results and GBC OCT-based results (P ≤ 0.01 for all comparisons).ConclusionDeep learning en face image analysis improves on feature-based GBC models for classifying healthy and glaucoma eyes.

To compare the diagnostic accuracy and explainability of a Vision Transformer deep learning technique, Data-efficient image Transformer (DeiT), and ResNet-50, trained on fundus photographs from the Ocular Hypertension Treatment Study (OHTS) to detect primary open-angle glaucoma (POAG) and identify the salient areas of the photographs most important for each model's decision-making process.Evaluation of a diagnostic technology.Overall 66 715 photographs from 1636 OHTS participants and an additional 5 external datasets of 16 137 photographs of healthy and glaucoma eyes.Data-efficient image Transformer models were trained to detect 5 ground-truth OHTS POAG classifications: OHTS end point committee POAG determinations because of disc changes (model 1), visual field (VF) changes (model 2), or either disc or VF changes (model 3) and Reading Center determinations based on disc (model 4) and VFs (model 5). The best-performing DeiT models were compared with ResNet-50 models on OHTS and 5 external datasets.Diagnostic performance was compared using areas under the receiver operating characteristic curve (AUROC) and sensitivities at fixed specificities. The explainability of the DeiT and ResNet-50 models was compared by evaluating the attention maps derived directly from DeiT to 3 gradient-weighted class activation map strategies.Compared with our best-performing ResNet-50 models, the DeiT models demonstrated similar performance on the OHTS test sets for all 5 ground-truth POAG labels; AUROC ranged from 0.82 (model 5) to 0.91 (model 1). Data-efficient image Transformer AUROC was consistently higher than ResNet-50 on the 5 external datasets. For example, AUROC for the main OHTS end point (model 3) was between 0.08 and 0.20 higher in the DeiT than ResNet-50 models. The saliency maps from the DeiT highlight localized areas of the neuroretinal rim, suggesting important rim features for classification. The same maps in the ResNet-50 models show a more diffuse, generalized distribution around the optic disc.Vision Transformers have the potential to improve generalizability and explainability in deep learning models, detecting eye disease and possibly other medical conditions that rely on imaging for clinical diagnosis and management.

Purpose: To compare the diagnostic accuracy and explainability of a new Vision Transformer deep learning technique, Data-efficient image Transformer (DeiT), and Resnet-50, trained on fundus photographs from the Ocular Hypertension Treatment Study (OHTS) to detect primary open-angle glaucoma (POAG) and to identify the salient areas of the photographs most important for each model's decision-making process. Study Design: Evaluation of a diagnostic technology Subjects, Participants, and/or Controls: 66,715 photographs from 1,636 OHTS participants and an additional five external datasets of 16137 photographs of healthy and glaucoma eyes. Methods, Intervention, or Testing: DeiT models were trained to detect five ground truth OHTS POAG classifications: OHTS Endpoint Committee POAG determinations due to disc changes (Model 1), visual field changes (Model 2), or either disc or visual field changes (Model 3) and reading center determinations based on disc (Model 4) and visual fields (Model 5). The best-performing DeiT models were compared to ResNet-50 on OHTS and five external datasets. Main Outcome Measures: Diagnostic performance was compared using areas under the receiver operating characteristic curve (AUROC) and sensitivities at fixed specificities. The explainability of the DeiT and ResNet-50 models was compared by evaluating the attention maps derived directly from DeiT to 3 gradient-weighted class activation map generation strategies. Results: Compared to our best-performing ResNet-50 models, the DeiT models demonstrated similar performance on the OHTS test sets for all five-ground truth POAG labels; AUROC ranged from 0.82 (Model 5) to 0.91 (Model 1). However, the AUROC of DeiT was consistently higher than ResNet-50 on the five external datasets. For example, AUROC for the main OHTS endpoint (Model 3) was between 0.08 and 0.20 higher in the DeiT compared to ResNet-50 models. The saliency maps from the DeiT highlight localized areas of the neuroretinal rim, suggesting the use of important clinical features for classification, while the same maps in the ResNet-50 models show a more diffuse, generalized distribution around the optic disc, Conclusions: Vision transformer has the potential to improve the generalizability and explainability of deep learning models for the detection of eye disease and possibly other medical conditions that rely on imaging modalities for clinical diagnosis and management.

Purpose To compare gradient-boosting classifier (GBC) analysis of optical coherence tomography angiography (OCTA)-measured vessel density (VD) and OCT-measured tissue thickness to standard OCTA VD and OCT thickness parameters for classifying healthy eyes and eyes with early to moderate glaucoma. Design Comparison of diagnostic tools. Methods A total of 180 healthy eyes and 193 glaucomatous eyes with OCTA and OCT imaging of the macula and optic nerve head (ONH) were studied. Four GBCs were evaluated that combined 1) all macula VD and thickness measurements (Macula GBC), 2) all ONH VD and thickness measurements (ONH GBC), 3) all VD measurements from the macula and ONH (vessel density GBC), and 4) all thickness measurements from the macula and ONH (thickness GBC). ROC curve (AUROC) analyses compared the diagnostic accuracy of GBCs to that of standard instrument-provided parameters. A fifth GBC that combined all parameters (full GBC) also was investigated. Results GBCs had better diagnostic accuracy than standard OCTA and OCT parameters with AUROCs ranging from 0.90 to 0.93 and 0.64 to 0.91, respectively. The full GBC (AUROC = 0.93) performed significantly better than the ONH GBC (AUROC = 0.91; P = .036) and the vessel density GBC (AUROC = 0.90; P = .010). All other GBCs performed similarly. The mean relative influence of each parameter included in the full GBC identified a combination of macular thickness and ONH VD measurements as the greatest contributors. Conclusions GBCs that combine OCTA and OCT macula and ONH measurements can improve diagnostic accuracy for glaucoma detection compared to most but not all instrument provided parameters.

Purpose To assess the accuracy of deep learning models to predict glaucoma development from fundus photographs several years before disease onset. Design Algorithm development for predicting glaucoma using data from a prospective longitudinal study. Participants A total of 66 721 fundus photographs from 3272 eyes of 1636 subjects who participated in the Ocular Hypertension Treatment Study (OHTS) were included. Main Outcome Measures Accuracy and area under the curve (AUC). Methods Fundus photographs and visual fields were carefully examined by 2 independent readers from the optic disc and visual field reading centers of the OHTS. When an abnormality was detected by the readers, the subject was recalled for retesting to confirm the abnormality and for further confirmation by an end point committee. By using 66 721 fundus photographs, deep learning models were trained and validated using 85% of the fundus photographs and further retested (validated) on the remaining (held-out) 15% of the fundus photographs. Results The AUC of the deep learning model in predicting glaucoma development 4 to 7 years before disease onset was 0.77 (95% confidence interval [CI], 0.75–0.79). The accuracy of the model in predicting glaucoma development approximately 1 to 3 years before disease onset was 0.88 (95% CI, 0.86–0.91). The accuracy of the model in detecting glaucoma after onset was 0.95 (95% CI, 0.94–0.96). Conclusions Deep learning models can predict glaucoma development before disease onset with reasonable accuracy. Eyes with visual field abnormality but not glaucomatous optic neuropathy had a higher tendency to be missed by deep learning algorithms.

Chromosomal instability (CIN) and epigenetic alterations have been implicated in tumor progression and metastasis; yet how these two hallmarks of cancer are related remains poorly understood. By integrating genetic, epigenetic, and functional analyses at the single cell level, we show that progression of uveal melanoma (UM), the most common intraocular primary cancer in adults, is driven by loss of Polycomb Repressive Complex 1 (PRC1) in a subpopulation of tumor cells. This leads to transcriptional de-repression of PRC1-target genes and mitotic chromosome segregation errors. Ensuing CIN leads to the formation of rupture-prone micronuclei, exposing genomic double-stranded DNA (dsDNA) to the cytosol. This provokes tumor cell-intrinsic inflammatory signaling, mediated by aberrant activation of the cGAS-STING pathway. PRC1 inhibition promotes nuclear enlargement, induces a transcriptional response that is associated with significantly worse patient survival and clinical outcomes, and enhances migration that is rescued upon pharmacologic inhibition of CIN or STING. Thus, deregulation of PRC1 can promote tumor progression by inducing CIN and represents an opportunity for early therapeutic intervention., The molecular underpinnings driving uveal melanoma (UM) progression are unknown. Here the authors show that loss of Polycomb Repressive Complex 1 triggers chromosomal instability, which promotes inflammatory signaling and migration in UM.

To investigate the diagnostic accuracy of deep learning (DL) algorithms to detect primary open-angle glaucoma (POAG) trained on fundus photographs from the Ocular Hypertension Treatment Study (OHTS). 66,715 photographs from 3,272 eyes were used to train and test a ResNet-50 model to detect the OHTS Endpoint Committee POAG determination based on optic disc (n=287 eyes, 3,502 photographs) and/or visual field (n=198 eyes, 2,300 visual fields) changes. OHTS training, validation and testing sets were randomly determined using an 85-5-10 percentage split by subject. Three independent test sets were used to estimate the generalizability of the model: UCSD Diagnostic Innovations in Glaucoma Study (DIGS, USA), ACRIMA (Spain) and Large-scale Attention-based Glaucoma (LAG, China). The DL model achieved an AUROC (95% CI) of 0.88 (0.82, 0.92) for the overall OHTS POAG endpoint. For the OHTS endpoints based on optic disc changes or visual field changes, AUROCs were 0.91 (0.88, 0.94) and 0.86 (0.76, 0.93), respectively. False-positive rates (at 90% specificity) were higher in photographs of eyes that later developed POAG by disc or visual field (19.1%) compared to eyes that did not develop POAG (7.3%) during their OHTS follow-up. The diagnostic accuracy of the DL model developed on the OHTS optic disc endpoint applied to 3 independent datasets was lower with AUROC ranging from 0.74 to 0.79. High diagnostic accuracy of the current model suggests that DL can be used to automate the determination of POAG for clinical trials and management. In addition, the higher false-positive rate in early photographs of eyes that later developed POAG suggests that DL models detected POAG in some eyes earlier than the OHTS Endpoint Committee.

Author(s): Bowd, Christopher; Belghith, Akram; Christopher, Mark; Goldbaum, Michael H; Fazio, Massimo A; Girkin, Christopher A; Liebmann, Jeffrey M; de Moraes, Carlos Gustavo; Weinreb, Robert N; Zangwill, Linda M | Abstract: PurposeTo compare change over time in eye-specific optical coherence tomography (OCT) retinal nerve fiber layer (RNFL)-based region-of-interest (ROI) maps developed using unsupervised deep-learning auto-encoders (DL-AE) to circumpapillary RNFL (cpRNFL) thickness for the detection of glaucomatous progression.MethodsForty-four progressing glaucoma eyes (by stereophotograph assessment), 189 nonprogressing glaucoma eyes (by stereophotograph assessment), and 109 healthy eyes were followed for ≥3 years with ≥4 visits using OCT. The San Diego Automated Layer Segmentation Algorithm was used to automatically segment the RNFL layer from raw three-dimensional OCT images. For each longitudinal series, DL-AEs were used to generate individualized eye-based ROI maps by identifying RNFL regions of likely progression and no change. Sensitivities and specificities for detecting change over time and rates of change over time were compared for the DL-AE ROI and global cpRNFL thickness measurements derived from a 2.22-mm to 3.45-mm annulus centered on the optic disc.ResultsThe sensitivity for detecting change in progressing eyes was greater for DL-AE ROIs than for global cpRNFL annulus thicknesses (0.90 and 0.63, respectively). The specificity for detecting not likely progression in nonprogressing eyes was similar (0.92 and 0.93, respectively). The mean rates of change in DL-AE ROI were significantly faster than for cpRNFL annulus thickness in progressing eyes (-1.28 µm/y vs. -0.83 µm/y) and nonprogressing eyes (-1.03 µm/y vs. -0.78 µm/y).ConclusionsEye-specific ROIs identified using DL-AE analysis of OCT images show promise for improving assessment of glaucomatous progression.Translational relevanceThe detection and monitoring of structural glaucomatous progression can be improved by considering eye-specific regions of likely progression identified using deep learning.

A 68-year-old woman with a recent history of blurring in the left eye had undergone mastectomy for breast cancer 20 years ago. A series of bone metastases started 5 years after her diagnosis. Examination of the optic nerve head of the left eye revealed an isolated peripapillary mass. Indocyanine green angiography displayed vessels within the mass, and fluorescein angiography demonstrated hyperfluorescence of the mass from vascular leakage plus lobular spots of blocked fluorescence. B-scan ultrasound revealed a hyperechoic-elevated nodular mass on the optic disc. Spectral-domain optical coherence tomography displayed a mass of spherules. Magnetic resonance imaging of the brain demonstrated metastatic tumors. She was diagnosed with an optic disk metastasis from her breast carcinoma.

G protein mutations are common in uveal melanomas, and the vast majority target amino acid residue Q209 in either GNAQ or GNA11. The GNAQ R183Q mutation is found in a small fraction of uveal melanomas. We report a patient with an unusual presentation of uveal melanoma arising at an early age in the setting of congenital skin and ocular surface melanosis. A 34-year-old Hispanic female with congenital bilateral nevus of Ota and ocular surface melanosis presented with progressive loss of visual acuity and was found to have a juxtapapillary uveal melanoma. She was treated with brachytherapy, but the tumor relapsed. She underwent enucleation that revealed mixed spindle and epithelioid uveal melanoma cells with no extraocular or lymphovascular spread. Next-generation sequencing performed on DNA isolated from the enucleation specimen identified a GNAQ R183Q mutation and a PMS1 truncation mutation. Cytogenetic profiling revealed no monosomy 3. These findings raise the possibility that uveal melanomas bearing G protein R183 mutations may have distinct clinicopathologic profiles compared to those with Q209 mutations. Furthermore, this is the first reported case of a mutation in the mismatch repair gene PMS1 associated with uveal melanoma.

We develop an Artificial Intelligence (AI) framework for glaucoma precognition from baseline confocal scanning laser ophthalmoscopy imaging data, using a convolutional neural network (CNN) model. The proposed framework extracts ‘deep features’ from convolutional layers of the CNN model, which are used as input to the ensemble learning classifier in order to identify patients that will likely convert to glaucoma after few years. The prediction model achieved area under the receiver operating characteristic curve (AUC) of 0.83 using the data from baseline visit. The model predicted the onset of glaucoma more accurately than known glaucoma risk factors, Glaucoma Probability Score (GPS) and Moorfields Regression Analysis (MRA) parameters of the Heidelberg Retinal Tomograph (HRT) software. The proposed AI construct provides a highly specific and sensitive model that can predict the onset of glaucoma from baseline HRT parameters and has the potential to provide clinicians valuable information regarding the onset of glaucoma.

BackgroundCardiovascular disease is the leading cause of mortality and disability worldwide. A noninvasive test that can detect underlying cardiovascular disease has the potential to identify patients at risk prior to the occurrence of adverse cardiovascular events. We sought to determine whether an easily observed imaging finding indicative of retinal ischemia, which we term 'retinal ischemic perivascular lesions' (RIPLs), could serve as a biomarker for cardiovascular disease.MethodsWe reviewed optical coherence tomography (OCT) scans of individuals, with no underlying retinal pathology, obtained at UC San Diego Health from July 2014 to July 2019. We identified 84 patients with documented cardiovascular disease and 76 healthy controls. OCT scans were assessed for evidence of RIPLs. In addition, the 10-year atherosclerotic cardiovascular disease (ASCVD) risk calculator was used to risk-stratify the subjects into four different categories.FindingsPatients with documented cardiovascular disease had higher number of RIPLs compared to healthy controls (2.8vs 0.8, p37). The number of RIPLs in individuals with intermediate and high 10-year ASCVD risk scores was higher than in those with low ASCVD risk scores (1.7vs 0.64, p=0.02 and 2.9vs 0.64, p 0.002, respectively).InterpretationThe presence of RIPLs, which are anatomical markers of prior retinal ischemic infarcts, is suggestive of coexisting cardiovascular disease. RIPLs detection, obtained from routine retinal scans, may thus provide an additional biomarker to identify patients at risk of developing adverse cardiovascular events.FundingNone.

IMPORTANCE: Automated deep learning (DL) analyses of fundus photographs potentially can reduce the cost and improve the efficiency of reading center assessment of end points in clinical trials. OBJECTIVE: To investigate the diagnostic accuracy of DL algorithms trained on fundus photographs from the Ocular Hypertension Treatment Study (OHTS) to detect primary open-angle glaucoma (POAG). DESIGN, SETTING, AND PARTICIPANTS: In this diagnostic study, 1636 OHTS participants from 22 sites with a mean (range) follow-up of 10.7 (0-14.3) years. A total of 66 715 photographs from 3272 eyes were used to train and test a ResNet-50 model to detect the OHTS Endpoint Committee POAG determination based on optic disc (287 eyes, 3502 photographs) and/or visual field (198 eyes, 2300 visual fields) changes. Three independent test sets were used to evaluate the generalizability of the model. MAIN OUTCOMES AND MEASURES: Areas under the receiver operating characteristic curve (AUROC) and sensitivities at fixed specificities were calculated to compare model performance. Evaluation of false-positive rates was used to determine whether the DL model detected POAG before the OHTS Endpoint Committee POAG determination. RESULTS: A total of 1147 participants were included in the training set (661 [57.6%] female; mean age, 57.2 years; 95% CI, 56.6-57.8), 167 in the validation set (97 [58.1%] female; mean age, 57.1 years; 95% CI, 55.6-58.7), and 322 in the test set (173 [53.7%] female; mean age, 57.2 years; 95% CI, 56.1-58.2). The DL model achieved an AUROC of 0.88 (95% CI, 0.82-0.92) for the OHTS Endpoint Committee determination of optic disc or VF changes. For the OHTS end points based on optic disc changes or visual field changes, AUROCs were 0.91 (95% CI, 0.88-0.94) and 0.86 (95% CI, 0.76-0.93), respectively. False-positive rates (at 90% specificity) were higher in photographs of eyes that later developed POAG by disc or visual field (27.5% [56 of 204]) compared with eyes that did not develop POAG (11.4% [50 of 440]) during follow-up. The diagnostic accuracy of the DL model developed on the optic disc end point applied to 3 independent data sets was lower, with AUROCs ranging from 0.74 (95% CI, 0.70-0.77) to 0.79 (95% CI, 0.78-0.81). CONCLUSIONS AND RELEVANCE: The model’s high diagnostic accuracy using OHTS photographs suggests that DL has the potential to standardize and automate POAG determination for clinical trials and management. In addition, the higher false-positive rate in early photographs of eyes that later developed POAG suggests that DL models detected POAG in some eyes earlier than the OHTS Endpoint Committee, reflecting the OHTS design that emphasized a high specificity for POAG determination by requiring a clinically significant change from baseline.

Purpose To develop deep learning (DL) systems estimating visual function from macula-centered spectral-domain (SD) OCT images. Design Evaluation of a diagnostic technology. Participants A total of 2408 10-2 visual field (VF) SD OCT pairs and 2999 24-2 VF SD OCT pairs collected from 645 healthy and glaucoma subjects (1222 eyes). Methods Deep learning models were trained on thickness maps from Spectralis macula SD OCT to estimate 10-2 and 24-2 VF mean deviation (MD) and pattern standard deviation (PSD). Individual and combined DL models were trained using thickness data from 6 layers (retinal nerve fiber layer [RNFL], ganglion cell layer [GCL], inner plexiform layer [IPL], ganglion cell-IPL [GCIPL], ganglion cell complex [GCC] and retina). Linear regression of mean layer thicknesses were used for comparison. Main Outcome Measures Deep learning models were evaluated using R2 and mean absolute error (MAE) compared with 10-2 and 24-2 VF measurements. Results Combined DL models estimating 10-2 achieved R2 of 0.82 (95% confidence interval [CI], 0.68–0.89) for MD and 0.69 (95% CI, 0.55–0.81) for PSD and MAEs of 1.9 dB (95% CI, 1.6–2.4 dB) for MD and 1.5 dB (95% CI, 1.2–1.9 dB) for PSD. This was significantly better than mean thickness estimates for 10-2 MD (0.61 [95% CI, 0.47–0.71] and 3.0 dB [95% CI, 2.5–3.5 dB]) and 10-2 PSD (0.46 [95% CI, 0.31–0.60] and 2.3 dB [95% CI, 1.8–2.7 dB]). Combined DL models estimating 24-2 achieved R2 of 0.79 (95% CI, 0.72–0.84) for MD and 0.68 (95% CI, 0.53–0.79) for PSD and MAEs of 2.1 dB (95% CI, 1.8–2.5 dB) for MD and 1.5 dB (95% CI, 1.3–1.9 dB) for PSD. This was significantly better than mean thickness estimates for 24-2 MD (0.41 [95% CI, 0.26–0.57] and 3.4 dB [95% CI, 2.7–4.5 dB]) and 24-2 PSD (0.38 [95% CI, 0.20–0.57] and 2.4 dB [95% CI, 2.0–2.8 dB]). The GCIPL (R2 = 0.79) and GCC (R2 = 0.75) had the highest performance estimating 10-2 and 24-2 MD, respectively. Conclusions Deep learning models improved estimates of functional loss from SD OCT imaging. Accurate estimates can help clinicians to individualize VF testing to patients.

Deep archetypal analysis (DAA) has recently been proposed as an unsupervised approach for discovering latent structures in data. However, while a few approaches have used classical archetypal analysis (AA), DAA has not been incorporated in medical image analysis as yet. The purpose of this study is to develop a precognition framework to identify preclinical signs of glaucomatous vision loss using convex representations derived from DAA. We first develop an AA structure and a novel DAA framework to recognize hidden patterns of visual functional loss, and then project visual field data over the identified patterns to obtain a representation for glaucoma precognition several years prior to disease onset. We then develop a glaucoma classification framework using class-balanced bagging with neural networks to address the class imbalance problem. In contrast to other classification approaches, DAA, applied to a unique prospective longitudinal dataset with approximately eight years of visual field tests from normal eyes that developed glaucoma, has allowed visualization of the early signs of glaucoma and development of a construct for glaucoma precognition. Our findings suggest that our proposed glaucoma precognition approach could significantly advance state-of-the-art glaucoma prediction.

Author(s): Christopher, Mark; Nakahara, Kenichi; Bowd, Christopher; Proudfoot, James A; Belghith, Akram; Goldbaum, Michael H; Rezapour, Jasmin; Weinreb, Robert N; Fazio, Massimo A; Girkin, Christopher A; Liebmann, Jeffrey M; De Moraes, Gustavo; Murata, Hiroshi; Tokumo, Kana; Shibata, Naoto; Fujino, Yuri; Matsuura, Masato; Kiuchi, Yoshiaki; Tanito, Masaki; Asaoka, Ryo; Zangwill, Linda M | Abstract: PurposeTo compare performance of independently developed deep learning algorithms for detecting glaucoma from fundus photographs and to evaluate strategies for incorporating new data into models.MethodsTwo fundus photograph datasets from the Diagnostic Innovations in Glaucoma Study/African Descent and Glaucoma Evaluation Study and Matsue Red Cross Hospital were used to independently develop deep learning algorithms for detection of glaucoma at the University of California, San Diego, and the University of Tokyo. We compared three versions of the University of California, San Diego, and University of Tokyo models: original (no retraining), sequential (retraining only on new data), and combined (training on combined data). Independent datasets were used to test the algorithms.ResultsThe original University of California, San Diego and University of Tokyo models performed similarly (area under the receiver operating characteristic curve = 0.96 and 0.97, respectively) for detection of glaucoma in the Matsue Red Cross Hospital dataset, but not the Diagnostic Innovations in Glaucoma Study/African Descent and Glaucoma Evaluation Study data (0.79 and 0.92; P l .001), respectively. Model performance was higher when classifying moderate-to-severe compared with mild disease (area under the receiver operating characteristic curve = 0.98 and 0.91; P l .001), respectively. Models trained with the combined strategy generally had better performance across all datasets than the original strategy.ConclusionsDeep learning glaucoma detection can achieve high accuracy across diverse datasets with appropriate training strategies. Because model performance was influenced by the severity of disease, labeling, training strategies, and population characteristics, reporting accuracy stratified by relevant covariates is important for cross study comparisons.Translational relevanceHigh sensitivity and specificity of deep learning algorithms for moderate-to-severe glaucoma across diverse populations suggest a role for artificial intelligence in the detection of glaucoma in primary care.

Purpose Uveal melanomas are associated with characteristic genetic changes. Germline mutations in mismatch repair (MMR) genes and microsatellite instability have been implicated in the development of numerous malignant neoplasms such as colon and ovarian cancers. The frequency of MMR defects in uveal melanomas has yet to be determined. Methods Here, we analyzed the frequency of MMR gene mutations in uveal melanoma specimens from the University of California, San Diego (UCSD), The Cancer Genome Atlas (TGCA), and the Catalogue of Somatic Mutations in Cancer (COSMIC). Results We identified only two mutations in a MMR gene: one premature stop codon in the PMS gene within the UCSD cohort (0.5% frequency) and one in-frame deletion in MSH3 within the COSMIC database (0.8% frequency). We report copy number variation of MLH1 in monosomy 3 and show decreased mRNA expression of MLH1 in uveal melanoma specimens with monosomy 3. Expression levels of MLH1 were not found to correlate with the observed number of total mutations. Conclusion Overall, we show that mutations in MMR genes in uveal melanoma specimens are exceedingly rare, and although one copy of MLH1 is lost in monosomy 3, it does not seem to have pathologic consequences in uveal melanoma pathogenesis.